Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000319
(1023 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001192115.1| hypothetical protein LOC524810 [Bos taurus]. 304 2e-84
Alignment gi|XP_002696847.1| PREDICTED: IgM [Bos taurus]. 100 4e-21
Alignment gi|XP_002696848.1| PREDICTED: IgM [Bos taurus]. 99 5e-21
Alignment gi|XP_002684044.1| PREDICTED: Ig heavy chain-like, partial [Bos... 96 7e-20
Alignment gi|XP_002683686.1| PREDICTED: hypothetical protein [Bos taurus]. 80 3e-15
Alignment gi|NP_001077269.1| immunoglobulin lambda-like polypeptide 1 [Bo... 69 5e-12
Alignment gi|XP_002691399.1| PREDICTED: IGK protein-like [Bos taurus]. 52 1e-06
Alignment gi|XP_002700829.1| PREDICTED: IGK protein-like [Bos taurus]. 52 1e-06
Alignment gi|XP_002694682.1| PREDICTED: hCG96018-like [Bos taurus]. 50 4e-06
Alignment gi|XP_002701782.1| PREDICTED: immunoglobulin lambda-like polype... 49 7e-06
>ref|NP_001192115.1| hypothetical protein LOC524810 [Bos taurus].
Length = 487
Score = 304 bits (778), Expect(2) = 2e-84
Identities = 163/308 (52%), Positives = 201/308 (65%), Gaps = 16/308 (5%)
Frame = +2
Query: 86 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGL 259
W +LF L +GV + +L ESG LV+P +L L+C SGF+ SS + WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTASGFSLSSYGINWVRQAPGKAL 64
Query: 260 EWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGGS---- 427
E LA I T Y ++K R I++D S++ L ++S+ TEDTA YYC + +
Sbjct: 65 ECLACITGGGN-TAYNPALKSRLNITKDKSKSQVSLSLSSVTTEDTATYYCAKYSTDDCA 123
Query: 428 ---------LWGPGXXXXXXXXXXTSPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTV 580
WG G TSP IFPL+LG+++PAG VVI CLV+ FFPS PL+V
Sbjct: 124 CSSERNYIDAWGQGLLVTVSSESETSPSIFPLSLGNNDPAGQVVIGCLVQGFFPSAPLSV 183
Query: 581 TWSPSREGVIVRNFPPAQAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPC 760
TW+ + + V VRNFP AG LYTMSSQLTLP CP Q + CQVQHLSK+S++V VPC
Sbjct: 184 TWNQNGDSVSVRNFPAVLAGSLYTMSSQLTLPASLCPKGQSVTCQVQHLSKASKTVAVPC 243
Query: 761 KVLPSDP-CPQCCKPSLSLQPPALADLLLGSNASLTCTLSGLKKSEGVSFTWQPSGGKDA 937
+ S C C+PSLS+QPPAL DLLLGSNASLTCTLSGLK +EG SFTW P+GGK A
Sbjct: 244 IIQDSSSCCVPNCEPSLSVQPPALEDLLLGSNASLTCTLSGLKSAEGASFTWNPTGGKTA 303
Query: 938 VQASPTRD 961
VQ SP RD
Sbjct: 304 VQGSPKRD 311
Score = 28.1 bits (61), Expect(2) = 2e-84
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +1
Query: 985 SILPGCADPW 1014
S+LPGCADPW
Sbjct: 320 SVLPGCADPW 329
>ref|XP_002696847.1| PREDICTED: IgM [Bos taurus].
Length = 193
Score = 99.8 bits (247), Expect = 4e-21
Identities = 53/113 (46%), Positives = 72/113 (63%), Gaps = 2/113 (1%)
Frame = +2
Query: 86 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGL 259
W +LF L +GV + +L ESG LV+P +L L+C SGF+ S +V WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTVSGFSLSDNSVGWVRQAPGKAL 64
Query: 260 EWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
EWL I T Y ++K R +I++DNS++ L ++S+ TEDTA YYC R
Sbjct: 65 EWLGVI-YSGGSTGYNPALKSRLSITKDNSKSQVSLSLSSVTTEDTATYYCAR 116
>ref|XP_002696848.1| PREDICTED: IgM [Bos taurus].
Length = 259
Score = 99.4 bits (246), Expect = 5e-21
Identities = 53/117 (45%), Positives = 73/117 (62%), Gaps = 2/117 (1%)
Frame = +2
Query: 86 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGL 259
W +LF L +GV + +L ESG LV+P +L L+C SGF+ SS V WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTVSGFSLSSNGVGWVRQAPGKAL 64
Query: 260 EWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGGSL 430
EW+ I TYY ++K R +I++DNS++ L ++S+ EDTA YYC + L
Sbjct: 65 EWVGGI-DNDGDTYYNPALKSRLSITKDNSKSQVSLSVSSVTPEDTATYYCAKDTEL 120
>ref|XP_002684044.1| PREDICTED: Ig heavy chain-like, partial [Bos taurus].
Length = 121
Score = 95.5 bits (236), Expect = 7e-20
Identities = 52/111 (46%), Positives = 71/111 (63%), Gaps = 2/111 (1%)
Frame = +2
Query: 86 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGL 259
W +LF L +GV + +L ESG LV+P +L L+C SGF+ SS AV WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTISGFSLSSYAVGWVRQAPGKAL 64
Query: 260 EWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 412
EW+ I T ++K R +I++DNS++ L ++S+ TEDTA YYC
Sbjct: 65 EWVGGI-SSGGSTCLNPALKSRLSITKDNSKSQVSLSVSSVTTEDTATYYC 114
>ref|XP_002683686.1| PREDICTED: hypothetical protein [Bos taurus].
Length = 226
Score = 80.1 bits (196), Expect = 3e-15
Identities = 48/119 (40%), Positives = 70/119 (58%), Gaps = 4/119 (3%)
Frame = +2
Query: 86 WVVLFALLQGVQG---EEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVR-WVRQAPGK 253
W +L L+ G QG + +L ESG L++ +L L+C SG++ +S R W+RQAPGK
Sbjct: 4 WGLLLCLVTGPQGVLSQVQLQESGPELIKSSQTLSLTCAGSGYSITSDYGRNWIRQAPGK 63
Query: 254 GLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGGSL 430
GLE + + TYY+ S+K +I RD +N LQ++S+ TEDTA Y R +L
Sbjct: 64 GLEQMGCMYYNGD-TYYSPSIKSHTSICRDTPKNQFSLQLSSVPTEDTAMCYLARNTAL 121
>ref|NP_001077269.1| immunoglobulin lambda-like polypeptide 1 [Bos taurus].
Length = 235
Score = 69.3 bits (168), Expect = 5e-12
Identities = 60/222 (27%), Positives = 89/222 (40%), Gaps = 10/222 (4%)
Frame = +2
Query: 89 VVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWL 268
+ L AL G + L + G + ++C S V W +Q PG GL+ +
Sbjct: 8 LTLVALCTGSWAQAVLTQPSSVSGSLGQRVSITCSGSSTNIGIYGVNWYQQVPGSGLKTI 67
Query: 269 AYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGG-----SLW 433
Y Y V RF+ S+ S NTA L +NSL+ ED A Y+C G +++
Sbjct: 68 IY-----EDKYRPSGVPDRFSGSK--SGNTATLTINSLQAEDEADYFCAAGDYSVNTAVF 120
Query: 434 GPGXXXXXXXXXXTSPKIFPLTLGSSEPAG-YVVIACLVRDFFPSEPLTVTWSPSREGVI 610
G G + P + + E G + CL+ DF+P +TV W + I
Sbjct: 121 GGGTTLTVLGQPKSPPSVTLFPPSTEELNGNKATLVCLISDFYPGS-VTVVWK-ADGSTI 178
Query: 611 VRNFPPA----QAGGLYTMSSQLTLPVEQCPADQILKCQVQH 724
RN Q+ Y SS L+L + C+V H
Sbjct: 179 TRNVETTRASKQSNSKYAASSYLSLTSSDWKSKGSYSCEVTH 220
>ref|XP_002691399.1| PREDICTED: IGK protein-like [Bos taurus].
Length = 235
Score = 51.6 bits (122), Expect = 1e-06
Identities = 55/228 (24%), Positives = 89/228 (39%), Gaps = 9/228 (3%)
Frame = +2
Query: 92 VLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLA 271
+L L G +G+ ++ +S L G R+S + W +Q PGK + L
Sbjct: 10 LLLLWLPGKEGDIQVTQSPSSLSASLGD-RVSITCQASQSIDTKLAWYQQKPGKAPKLLI 68
Query: 272 YIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGG---SLWGPG 442
Y + +F+ S + T L ++SL+ +D A YYC + +G G
Sbjct: 69 YAIPRSPSWF-----PSQFSGSGFGADFT--LTISSLKADDIATYYCLSRNCCVNTFGQG 121
Query: 443 XXXXXXXXXXTSPKIFPLTLGSSE-PAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRN 619
P +F + G V + CLV DF+P + + V W N
Sbjct: 122 TKVEIKRSD-AEPSVFLFKPSDEQLKTGTVSVVCLVNDFYPKD-INVKWKVDGVTQSSSN 179
Query: 620 FPPA-----QAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKSSQSV 748
F + Y++SS LTLP + + C+V H S ++ V
Sbjct: 180 FQNSFTDQDSKKSTYSLSSILTLPSSEYQSHDAYTCEVSHKSLTTTLV 227
>ref|XP_002700829.1| PREDICTED: IGK protein-like [Bos taurus].
Length = 235
Score = 51.6 bits (122), Expect = 1e-06
Identities = 55/228 (24%), Positives = 89/228 (39%), Gaps = 9/228 (3%)
Frame = +2
Query: 92 VLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLA 271
+L L G +G+ ++ +S L G R+S + W +Q PGK + L
Sbjct: 10 LLLLWLPGKEGDIQVTQSPSSLSASLGD-RVSITCQASQSIDTKLAWYQQKPGKAPKLLI 68
Query: 272 YIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGG---SLWGPG 442
Y + +F+ S + T L ++SL+ +D A YYC + +G G
Sbjct: 69 YAIPRSPSWF-----PSQFSGSGFGADFT--LTISSLKADDIATYYCLSRNCCVNTFGQG 121
Query: 443 XXXXXXXXXXTSPKIFPLTLGSSE-PAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRN 619
P +F + G V + CLV DF+P + + V W N
Sbjct: 122 TKVEIKRSD-AEPSVFLFKPSDEQLKTGTVSVVCLVNDFYPKD-INVKWKVDGVTQSSSN 179
Query: 620 FPPA-----QAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKSSQSV 748
F + Y++SS LTLP + + C+V H S ++ V
Sbjct: 180 FQNSFTDQDSKKSTYSLSSILTLPSSEYQSHDAYTCEVSHKSLTTTLV 227
>ref|XP_002694682.1| PREDICTED: hCG96018-like [Bos taurus].
Length = 150
Score = 49.7 bits (117), Expect = 4e-06
Identities = 29/83 (34%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = +2
Query: 167 GGSLRLSC-VDSGFTFSSRAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRD 343
G S RLSC + SG+ S+ ++ W +Q G L +L + V RF+ S+D
Sbjct: 34 GASARLSCTLSSGYNVSNYSIYWYQQKAGNPLRYLLRFKSDSDK-HQGSGVPSRFSGSKD 92
Query: 344 NSQNTAYLQMNSLRTEDTARYYC 412
S N L ++ L+ ED A YYC
Sbjct: 93 ASTNAGLLLISGLQPEDEADYYC 115
>ref|XP_002701782.1| PREDICTED: immunoglobulin lambda-like polypeptide 1-like [Bos
taurus].
Length = 236
Score = 48.9 bits (115), Expect = 7e-06
Identities = 56/224 (25%), Positives = 81/224 (36%), Gaps = 14/224 (6%)
Frame = +2
Query: 95 LFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAY 274
L L G +L + V G + ++C G + W +Q PG+ + Y
Sbjct: 10 LLTLCTGHLASSQLTQPPAVSVSLGQTASITC--QGDDLELLSAHWYQQKPGQAPVLVIY 67
Query: 275 IXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGGSLWGP----- 439
A + RF+ S+ S TA L + + ED A YYC + + GP
Sbjct: 68 ADDNL-----ASGIPDRFSGSK--SDTTATLTIRGAQAEDEADYYC-QSADISGPVSLCD 119
Query: 440 ---GXXXXXXXXXXTSPKIFPLTLGSSEP--AGYVVIACLVRDFFPSEPLTVTWSPSREG 604
G SP L S E A + CL+ DF+P +TV W +
Sbjct: 120 LFGGGTRVTVLGQPKSPPSVTLFPPSKEELSANKATLVCLISDFYPGS-VTVAWK-ADGS 177
Query: 605 VIVRNFPPA----QAGGLYTMSSQLTLPVEQCPADQILKCQVQH 724
I RN Q+ Y SS L+L + C+V H
Sbjct: 178 TITRNVKTTRASKQSNSKYAASSYLSLTDSDWKSKGSYSCEVTH 221
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 48,368,049
Number of extensions: 1599460
Number of successful extensions: 8610
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 8462
Number of HSP's successfully gapped: 11
Length of query: 341
Length of database: 17,681,374
Length adjustment: 103
Effective length of query: 238
Effective length of database: 14,273,310
Effective search space: 3397047780
Effective search space used: 3397047780
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000319
(1023 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_849253.1| PREDICTED: similar to Ig heavy chain V-III regi... 180 2e-45
Alignment gi|XP_848432.1| PREDICTED: similar to Ig heavy chain V-III regi... 177 1e-44
Alignment gi|XP_848935.1| PREDICTED: similar to Ig heavy chain V-III regi... 176 2e-44
Alignment gi|XP_848877.1| PREDICTED: similar to Ig heavy chain V-III regi... 175 7e-44
Alignment gi|XP_848501.1| PREDICTED: similar to Ig heavy chain V-III regi... 174 1e-43
Alignment gi|XP_848616.1| PREDICTED: similar to Ig heavy chain V-III regi... 174 1e-43
Alignment gi|XP_848796.1| PREDICTED: similar to Ig heavy chain V-III regi... 172 5e-43
Alignment gi|XP_848599.1| PREDICTED: similar to Ig heavy chain V-III regi... 172 5e-43
Alignment gi|XP_848486.1| PREDICTED: similar to Ig heavy chain V-III regi... 170 2e-42
Alignment gi|XP_848979.1| PREDICTED: similar to Ig heavy chain V-III regi... 170 2e-42
>ref|XP_849253.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 142
Score = 180 bits (456), Expect = 2e-45
Identities = 92/136 (67%), Positives = 109/136 (80%)
Frame = +2
Query: 35 SVIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFS 214
S IR E+R ME + WV L A+L+GVQGE +LVESGG LV+PGGSLRLSCV SGFTFS
Sbjct: 3 SGIRVENRRLNMESFVGWVFLVAILKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFS 62
Query: 215 SRAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTED 394
S A+ WVRQAPGKGL+W+AYI TYYAD+VKGRFTISRDN++NT YLQMNSLR+ED
Sbjct: 63 SYAMSWVRQAPGKGLQWVAYINSGGSSTYYADAVKGRFTISRDNARNTLYLQMNSLRSED 122
Query: 395 TARYYCFRGGSLWGPG 442
TA YYC + +++ PG
Sbjct: 123 TAVYYCPK-DTVFSPG 137
>ref|XP_848432.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 267
Score = 177 bits (450), Expect = 1e-44
Identities = 87/124 (70%), Positives = 99/124 (79%)
Frame = +2
Query: 50 EHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVR 229
EHR ME L+WV L +L+GVQGE +LVESGG LV+PGGSLRLSCV SGFTFSS +
Sbjct: 104 EHRQRTMESALSWVFLVTILKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFSSYHMS 163
Query: 230 WVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYY 409
WVRQAPGKGL+W+AYI T YAD+VKGRFTISRDN++NT YLQMNSLR EDTA YY
Sbjct: 164 WVRQAPGKGLQWVAYINSGGSSTSYADAVKGRFTISRDNAKNTLYLQMNSLRAEDTAVYY 223
Query: 410 CFRG 421
C +G
Sbjct: 224 CAKG 227
>ref|XP_848935.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 315
Score = 176 bits (447), Expect = 2e-44
Identities = 87/126 (69%), Positives = 98/126 (77%)
Frame = +2
Query: 35 SVIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFS 214
SVIR +HR ME L WV L A+LQGVQGE +LVESGG L +PGGSLRLSCV SG TFS
Sbjct: 156 SVIRTQHRQITMESVLGWVFLVAILQGVQGEVQLVESGGDLAKPGGSLRLSCVASGLTFS 215
Query: 215 SRAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTED 394
S ++ WVRQAPGKGL+W+ I TYY D+VKGRFTISRDN++NT YLQMNSLR ED
Sbjct: 216 SYSMSWVRQAPGKGLQWVTAISYDGSSTYYTDAVKGRFTISRDNARNTVYLQMNSLRAED 275
Query: 395 TARYYC 412
TA YYC
Sbjct: 276 TAVYYC 281
>ref|XP_848877.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 250
Score = 175 bits (443), Expect = 7e-44
Identities = 86/125 (68%), Positives = 99/125 (79%)
Frame = +2
Query: 38 VIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSS 217
+IR + R ME L+WV L A+LQGVQGE +LVESGG LV+PGGSLRLSCV SGFTFS+
Sbjct: 108 LIRTQQRQTTMESVLSWVFLVAILQGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFSN 167
Query: 218 RAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 397
+ WVRQAPGKGL+W+A I TYY D+VKGRFTISRDN++NT YLQMNSLR EDT
Sbjct: 168 YDMSWVRQAPGKGLQWVAAISYDGSSTYYTDAVKGRFTISRDNARNTVYLQMNSLRAEDT 227
Query: 398 ARYYC 412
A YYC
Sbjct: 228 AVYYC 232
Score = 125 bits (315), Expect(2) = 3e-29
Identities = 61/89 (68%), Positives = 68/89 (76%)
Frame = +2
Query: 146 GGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGR 325
G LV+PGGSLRLSCV SGFTFS A+ WV QAPGKGL+W+A I TYY D+VKGR
Sbjct: 10 GEDLVKPGGSLRLSCVTSGFTFSRYAMSWVGQAPGKGLQWVAAISSSGSSTYYVDAVKGR 69
Query: 326 FTISRDNSQNTAYLQMNSLRTEDTARYYC 412
FTIS DN++N YLQMNSLR ED A YYC
Sbjct: 70 FTISIDNAKNMVYLQMNSLRAEDIAVYYC 98
Score = 21.6 bits (44), Expect(2) = 3e-29
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = +3
Query: 123 VRRSWWSLEEAWCSLGG 173
V+ SWWSL E GG
Sbjct: 2 VKCSWWSLGEDLVKPGG 18
>ref|XP_848501.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 162
Score = 174 bits (441), Expect = 1e-43
Identities = 87/126 (69%), Positives = 100/126 (79%)
Frame = +2
Query: 41 IRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSR 220
IR E+R M+ L WV L A+L+GVQGE +LVESGG LV+PGGSLRLSCV SGFTFSS
Sbjct: 4 IRTEYRQLTMKLVLGWVFLVAILKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFSSY 63
Query: 221 AVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA 400
+ WVRQAPGKGL+W+A+I T YAD+VKGRFTISRDN++NT YLQMNSLR EDTA
Sbjct: 64 NMGWVRQAPGKGLQWVAWIYDSGSSTSYADAVKGRFTISRDNAKNTLYLQMNSLRAEDTA 123
Query: 401 RYYCFR 418
YYC R
Sbjct: 124 VYYCAR 129
>ref|XP_848616.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 186
Score = 174 bits (441), Expect = 1e-43
Identities = 91/139 (65%), Positives = 103/139 (74%), Gaps = 4/139 (2%)
Frame = +2
Query: 38 VIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSS 217
VIR +HR ME L+WV L A+LQGVQGE +LVESGG LV+P GSLRLSCV SGFTFSS
Sbjct: 22 VIRTQHRQITMESVLSWVFLVAILQGVQGEVQLVESGGDLVKPAGSLRLSCVASGFTFSS 81
Query: 218 RAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 397
++ WVRQAP KGL+ +A I TYY D+VKGRFTISRDN++NT YLQMNSLR EDT
Sbjct: 82 YSMSWVRQAPEKGLQLVAGINSGGSSTYYTDAVKGRFTISRDNAKNTVYLQMNSLRAEDT 141
Query: 398 ARYYCFRGG----SLWGPG 442
A YYC G S GPG
Sbjct: 142 AMYYCAEAGSGQVSCQGPG 160
>ref|XP_848796.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 244
Score = 172 bits (436), Expect = 5e-43
Identities = 85/123 (69%), Positives = 97/123 (78%)
Frame = +2
Query: 44 RKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRA 223
+ EH+ ME L WV L A+L+GVQ E +LVESGG LV+P GSLRLSCV SGFTFSS
Sbjct: 70 KTEHKQLTMESVLCWVFLVAILKGVQSEVQLVESGGDLVKPVGSLRLSCVASGFTFSSYD 129
Query: 224 VRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTAR 403
+ WVRQAPGKGL+W+AYI TYYAD+VKGRFTISRDN++NT YLQMNSLR EDTA
Sbjct: 130 MNWVRQAPGKGLQWVAYISSGGSSTYYADAVKGRFTISRDNAKNTLYLQMNSLRAEDTAM 189
Query: 404 YYC 412
YYC
Sbjct: 190 YYC 192
>ref|XP_848599.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 201
Score = 172 bits (436), Expect = 5e-43
Identities = 88/133 (66%), Positives = 100/133 (75%)
Frame = +2
Query: 41 IRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSR 220
IR+E R ME L+WV L +L+GVQ E +LVESGG LV+P GSLRLSCV SGFTFSS
Sbjct: 40 IRRELRKLTMESVLSWVFLVTILKGVQCEVQLVESGGDLVKPEGSLRLSCVVSGFTFSSY 99
Query: 221 AVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA 400
+ WVRQAPGKGL+W+AYI T Y D+VKGRFTISRDN++NT YLQMNSLRTEDTA
Sbjct: 100 DMSWVRQAPGKGLQWVAYISSDGRSTSYTDAVKGRFTISRDNAKNTLYLQMNSLRTEDTA 159
Query: 401 RYYCFRGGSLWGP 439
YYC G W P
Sbjct: 160 VYYCAAGSLGWFP 172
>ref|XP_848486.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 239
Score = 170 bits (431), Expect = 2e-42
Identities = 86/126 (68%), Positives = 99/126 (78%)
Frame = +2
Query: 35 SVIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFS 214
S I EHR M L+WV L A+++GVQGE +LVESGG LV+PGGSLRLSCV SGFTFS
Sbjct: 57 SGIWTEHRQLTMTSVLSWVFLAAIVKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFS 116
Query: 215 SRAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTED 394
+ + WVRQAPGKGLEW+A I TYYA++VKGRFTISRDN++N AYLQMNSLR ED
Sbjct: 117 NYEMYWVRQAPGKGLEWVARIYESGSTTYYAEAVKGRFTISRDNAKNMAYLQMNSLRAED 176
Query: 395 TARYYC 412
TA YYC
Sbjct: 177 TAVYYC 182
>ref|XP_848979.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 168
Score = 170 bits (430), Expect = 2e-42
Identities = 85/125 (68%), Positives = 98/125 (78%)
Frame = +2
Query: 38 VIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSS 217
+I+ E R ME L+W+ L A+L+GVQGE +LVESGG LV+PGGSLRLSCV SGFTFSS
Sbjct: 7 MIKTEDRHLTMETVLSWIFLVAILKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFSS 66
Query: 218 RAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 397
+ WVRQAPGKGL+WL I T YAD+VKGRFTISRDN++NT YLQMNSLR EDT
Sbjct: 67 NDMDWVRQAPGKGLQWLTRISNDGRSTGYADAVKGRFTISRDNAKNTLYLQMNSLRAEDT 126
Query: 398 ARYYC 412
A YYC
Sbjct: 127 AVYYC 131
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 50,414,611
Number of extensions: 1634683
Number of successful extensions: 8534
Number of sequences better than 1.0e-05: 116
Number of HSP's gapped: 8408
Number of HSP's successfully gapped: 127
Length of query: 341
Length of database: 18,874,504
Length adjustment: 103
Effective length of query: 238
Effective length of database: 15,440,896
Effective search space: 3674933248
Effective search space used: 3674933248
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000319
(1023 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_003403902.1| PREDICTED: ig heavy chain V-III region VH26-... 170 2e-42
Alignment gi|XP_003403879.1| PREDICTED: ig heavy chain V-III region VH26-... 169 3e-42
Alignment gi|XP_003403714.1| PREDICTED: hypothetical protein LOC100653084... 169 3e-42
Alignment gi|XP_003403513.1| PREDICTED: ig heavy chain V-III region VH26-... 169 5e-42
Alignment gi|XP_003403901.1| PREDICTED: ig heavy chain V-III region VH26-... 168 6e-42
Alignment gi|XP_001718089.4| PREDICTED: hypothetical protein LOC100132941... 167 2e-41
Alignment gi|XP_002346315.2| PREDICTED: ig heavy chain V-III region VH26-... 167 2e-41
Alignment gi|XP_003120167.2| PREDICTED: hypothetical protein LOC100291917... 160 2e-39
Alignment gi|XP_003119639.1| PREDICTED: hypothetical protein LOC100508797... 157 1e-38
Alignment gi|XP_001718156.4| PREDICTED: ig heavy chain V-III region VH26-... 155 7e-38
>ref|XP_003403902.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 231
Score = 170 bits (430), Expect = 2e-42
Identities = 85/117 (72%), Positives = 97/117 (82%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV+L A+L+ VQ E +LVESGGGLVQPGGSLRLSC SGFTFSS A+ WVRQAP
Sbjct: 1 MEFWLSWVLLVAILKDVQCEVQLVESGGGLVQPGGSLRLSCSASGFTFSSYAMHWVRQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
GKGLE+++ I TYYADSVKGRFTISRDNS+NT YLQM+SLR EDTA YYC +
Sbjct: 61 GKGLEYVSAISSNGGSTYYADSVKGRFTISRDNSKNTLYLQMSSLRAEDTAVYYCVK 117
>ref|XP_003403879.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 173
Score = 169 bits (429), Expect = 3e-42
Identities = 85/117 (72%), Positives = 93/117 (79%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV L ALL+GVQ + +LVESGGG+VQPG SLRLSC SGFTFSS + WVRQAP
Sbjct: 1 MEFGLSWVFLVALLRGVQCQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
GKGLEW+A I YYADSVKGRFTISRDNS+NT YLQMNSLR DTA YYC R
Sbjct: 61 GKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAGDTAVYYCAR 117
>ref|XP_003403714.1| PREDICTED: hypothetical protein LOC100653084 [Homo sapiens].
Length = 279
Score = 169 bits (429), Expect = 3e-42
Identities = 83/115 (72%), Positives = 93/115 (80%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV L A+L+GVQ E +LVESGG +VQPGGSLRLSC SGFTF A+ WVRQAP
Sbjct: 1 MEFGLSWVFLVAILKGVQCEVQLVESGGVVVQPGGSLRLSCAASGFTFDDYAMHWVRQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 412
GKGLEW++ I TYYADSVKGRFTISRDNS+N+ YLQMNSLR EDTA YYC
Sbjct: 61 GKGLEWVSLISWDGGSTYYADSVKGRFTISRDNSKNSLYLQMNSLRAEDTALYYC 115
Score = 85.9 bits (211), Expect = 5e-17
Identities = 48/92 (52%), Positives = 58/92 (63%), Gaps = 2/92 (2%)
Frame = +2
Query: 164 PGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAYIXXXXXX--TYYADSVKGRFTIS 337
PG S + SGFTFS + W+RQA GKGLEW+ I T A SVKGRFTIS
Sbjct: 123 PGESWQGEGYSSGFTFSDFWMHWIRQASGKGLEWVGRIRTKRNSYTTECAASVKGRFTIS 182
Query: 338 RDNSQNTAYLQMNSLRTEDTARYYCFRGGSLW 433
RD+S+NT Y+QMN+L+T+ TA YY G W
Sbjct: 183 RDDSKNTLYMQMNTLKTKYTAIYYSPGQGLEW 214
Score = 56.6 bits (135), Expect = 4e-08
Identities = 28/66 (42%), Positives = 38/66 (57%)
Frame = +2
Query: 242 APGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRG 421
+PG+GLEW+ +I Y GRF S D S + AYLQ++SL+ EDTA Y R
Sbjct: 207 SPGQGLEWMGWIITYTGNPMYTHGFTGRFVFSMDTSVSMAYLQISSLKAEDTAVYDSMR- 265
Query: 422 GSLWGP 439
++W P
Sbjct: 266 DTVWKP 271
>ref|XP_003403513.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 147
Score = 169 bits (427), Expect = 5e-42
Identities = 85/117 (72%), Positives = 96/117 (82%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV L A+L+GVQ E +LV+SGGGLVQPGGSLRLSC SGFTFSS A+ WVRQAP
Sbjct: 1 MEFVLSWVFLVAILKGVQCEVQLVQSGGGLVQPGGSLRLSCAGSGFTFSSYAMHWVRQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
GKGLEW++ I TYYADSVKGRFTISRDN++N+ YLQMNSLR ED A YYC R
Sbjct: 61 GKGLEWVSAI-GTGGGTYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDMAVYYCAR 116
>ref|XP_003403901.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 186
Score = 168 bits (426), Expect = 6e-42
Identities = 81/117 (69%), Positives = 95/117 (81%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV L A+++GVQ + +LVESGGGLV+PGGSLRLSC SGFTFS + W+RQAP
Sbjct: 1 MEFGLSWVFLVAIIKGVQCQVQLVESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIRQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
GKGLEW++YI T YADSVKGRFTISRDN++N+ YLQMNSLR EDTA YYC R
Sbjct: 61 GKGLEWVSYISSSSSYTNYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAR 117
>ref|XP_001718089.4| PREDICTED: hypothetical protein LOC100132941 [Homo sapiens].
Length = 297
Score = 167 bits (422), Expect = 2e-41
Identities = 84/117 (71%), Positives = 95/117 (81%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV L A+L+GVQ E +LV+SGGGLV PGGSLRLSC SGFTFSS A+ WVRQAP
Sbjct: 1 MEFVLSWVFLVAILKGVQCEVQLVQSGGGLVHPGGSLRLSCAGSGFTFSSYAMHWVRQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
GKGLEW++ I TYYADSVKGRFTISRDN++N+ YLQMNSLR ED A YYC R
Sbjct: 61 GKGLEWVSAI-GTGGGTYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDMAVYYCAR 116
>ref|XP_002346315.2| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 147
Score = 167 bits (422), Expect = 2e-41
Identities = 84/117 (71%), Positives = 95/117 (81%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV L A+L+GVQ E +LV+SGGGLV PGGSLRLSC SGFTFSS A+ WVRQAP
Sbjct: 1 MEFVLSWVFLVAILKGVQCEVQLVQSGGGLVHPGGSLRLSCAGSGFTFSSYAMHWVRQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
GKGLEW++ I TYYADSVKGRFTISRDN++N+ YLQMNSLR ED A YYC R
Sbjct: 61 GKGLEWVSAI-GTGGGTYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDMAVYYCAR 116
>ref|XP_003120167.2| PREDICTED: hypothetical protein LOC100291917 [Homo sapiens].
Length = 295
Score = 160 bits (404), Expect = 2e-39
Identities = 81/126 (64%), Positives = 95/126 (75%)
Frame = +2
Query: 41 IRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSR 220
+ K + E L+W+ L A+L+GVQ E +LVESGGGLV+PGGSLRLSC SGFTFS
Sbjct: 13 LHKSEKRTTKESWLSWLFLVAILKGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFSDY 72
Query: 221 AVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA 400
+ WVRQAPGKGLEW++ I YYADSVKGRFTISRDN++N+ YLQMNSLR EDTA
Sbjct: 73 YMNWVRQAPGKGLEWVSSI-SSSSTIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTA 131
Query: 401 RYYCFR 418
YYC R
Sbjct: 132 VYYCAR 137
Score = 86.3 bits (212), Expect = 4e-17
Identities = 49/111 (44%), Positives = 64/111 (57%), Gaps = 2/111 (1%)
Frame = +2
Query: 116 VQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVR--WVRQAPGKGLEWLAYIXXXX 289
V + L ESG LV+P +L L+C SGF+ S+ +R W+RQ PGK LEWLA I
Sbjct: 164 VLSQVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGMRVSWIRQPPGKALEWLARIDWDD 223
Query: 290 XXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGGSLWGPG 442
+Y+ S+K R TIS+D S+N L M ++ DTA YYC R PG
Sbjct: 224 DK-FYSTSLKTRLTISKDTSKNQVVLTMTNMDPVDTATYYCARIPQRHSPG 273
>ref|XP_003119639.1| PREDICTED: hypothetical protein LOC100508797 [Homo sapiens].
Length = 309
Score = 157 bits (397), Expect = 1e-38
Identities = 86/140 (61%), Positives = 99/140 (70%), Gaps = 4/140 (2%)
Frame = +2
Query: 11 RSPAPGS----RSVIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSL 178
RSP+ G SVIR EH EF L+WV L A+L+ VQ E++LVESGGGLVQPGGSL
Sbjct: 108 RSPSLGIPKCLHSVIRTEHTGLTREFVLSWVFLVAILKCVQCEDQLVESGGGLVQPGGSL 167
Query: 179 RLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNT 358
R SC SGF FSS + WVR+APGKG EW++ I TYYADSV GRFTISRDN++ +
Sbjct: 168 RPSCAASGFAFSSYVLHWVRRAPGKGPEWVSAI-GTGGDTYYADSVMGRFTISRDNAKKS 226
Query: 359 AYLQMNSLRTEDTARYYCFR 418
YLQMNSL ED A YYC R
Sbjct: 227 LYLQMNSLIAEDMAVYYCAR 246
>ref|XP_001718156.4| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 148
Score = 155 bits (391), Expect = 7e-38
Identities = 78/117 (66%), Positives = 87/117 (74%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEF L+WV L A+LQGV E +LVESG GL QPGG L+LS SGFT S + W+ QAP
Sbjct: 1 MEFELSWVFLVAILQGVHCEVQLVESGRGLAQPGGYLKLSGAASGFTVGSWYMSWIHQAP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
GKGLEW++YI T YADSVKGRFTIS DNS+NT YLQMNSLR EDTA YYC R
Sbjct: 61 GKGLEWVSYISSSGCSTNYADSVKGRFTISTDNSKNTLYLQMNSLRVEDTAVYYCAR 117
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 49,331,736
Number of extensions: 1603974
Number of successful extensions: 8504
Number of sequences better than 1.0e-05: 18
Number of HSP's gapped: 8402
Number of HSP's successfully gapped: 21
Length of query: 341
Length of database: 18,297,164
Length adjustment: 103
Effective length of query: 238
Effective length of database: 14,901,872
Effective search space: 3546645536
Effective search space used: 3546645536
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000319
(1023 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_001472141.2| PREDICTED: hypothetical protein LOC16061 [Mu... 173 7e-44
Alignment gi|XP_003085513.1| PREDICTED: ig alpha chain C region [Mus musc... 166 6e-42
Alignment gi|XP_001474075.2| PREDICTED: ig alpha chain C region isoform 1... 166 6e-42
Alignment gi|XP_001478032.2| PREDICTED: hypothetical protein LOC100047361... 108 5e-24
Alignment gi|XP_003086829.1| PREDICTED: similar to immunoglobulin mu-chai... 103 3e-22
Alignment gi|XP_923669.1| PREDICTED: putative V-set and immunoglobulin do... 102 6e-22
Alignment gi|XP_003086828.1| PREDICTED: hypothetical protein LOC100505002... 98 1e-20
Alignment gi|XP_001474061.2| PREDICTED: hypothetical protein LOC100045292... 91 1e-18
>ref|XP_001472141.2| PREDICTED: hypothetical protein LOC16061 [Mus musculus].
Length = 349
Score = 173 bits (438), Expect(2) = 7e-44
Identities = 88/159 (55%), Positives = 110/159 (69%), Gaps = 3/159 (1%)
Frame = +2
Query: 476 SPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRNFPPAQA-GGLYT 652
+P I+PLTL + + V+I CL+ D+FPS + VTW S + + NFPPA A GG YT
Sbjct: 10 NPTIYPLTLPRALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGGYT 69
Query: 653 MSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPCK--VLPSDPCPQCCKPSLSLQPPA 826
MSSQLTLP +CP + +KC VQH S + Q +NV C P+ P P C+PSLSLQ PA
Sbjct: 70 MSSQLTLPAVECPEGESVKCSVQHDSNAVQELNVKCSGPPPPTTPPPPSCQPSLSLQRPA 129
Query: 827 LADLLLGSNASLTCTLSGLKKSEGVSFTWQPSGGKDAVQ 943
L DLLLGS+AS+TCTL+GL+ EG FTW+PS GKDAVQ
Sbjct: 130 LEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQ 168
Score = 23.1 bits (48), Expect(2) = 7e-44
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +1
Query: 985 SILPGCADPW 1014
S+LPGCA+ W
Sbjct: 183 SVLPGCAERW 192
>ref|XP_003085513.1| PREDICTED: ig alpha chain C region [Mus musculus].
Length = 413
Score = 166 bits (421), Expect(2) = 6e-42
Identities = 87/160 (54%), Positives = 109/160 (68%), Gaps = 4/160 (2%)
Frame = +2
Query: 476 SPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRNFPPAQA-GGLYT 652
+P I+PLTL + + V+I CL+ D+FPS + VTW S + + NFPPA A GG YT
Sbjct: 74 NPTIYPLTLPPALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGRYT 133
Query: 653 MSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPCKVLPSDPCP---QCCKPSLSLQPP 823
MSSQLTLP +CP + +KC VQH S Q ++V C P+ P P C+PSLSLQ P
Sbjct: 134 MSSQLTLPAVECPEGESVKCSVQHDSNPVQELDVNCSG-PTPPPPITIPSCQPSLSLQRP 192
Query: 824 ALADLLLGSNASLTCTLSGLKKSEGVSFTWQPSGGKDAVQ 943
AL DLLLGS+AS+TCTL+GL+ EG FTW+PS GKDAVQ
Sbjct: 193 ALEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQ 232
Score = 23.1 bits (48), Expect(2) = 6e-42
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +1
Query: 985 SILPGCADPW 1014
S+LPGCA+ W
Sbjct: 247 SVLPGCAERW 256
Score = 99.8 bits (247), Expect = 3e-21
Identities = 48/68 (70%), Positives = 52/68 (76%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
M+ LNWV L LL G+Q E KLVESGGGLVQPGGSLRLSC SGFTFS + WVRQ P
Sbjct: 1 MKLWLNWVFLLTLLHGIQCEVKLVESGGGLVQPGGSLRLSCATSGFTFSDFYMEWVRQPP 60
Query: 248 GKGLEWLA 271
GK LEW+A
Sbjct: 61 GKRLEWIA 68
>ref|XP_001474075.2| PREDICTED: ig alpha chain C region isoform 1 [Mus musculus].
Length = 354
Score = 166 bits (421), Expect(2) = 6e-42
Identities = 87/160 (54%), Positives = 109/160 (68%), Gaps = 4/160 (2%)
Frame = +2
Query: 476 SPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRNFPPAQA-GGLYT 652
+P I+PLTL + + V+I CL+ D+FPS + VTW S + + NFPPA A GG YT
Sbjct: 15 NPTIYPLTLPPALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGRYT 74
Query: 653 MSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPCKVLPSDPCP---QCCKPSLSLQPP 823
MSSQLTLP +CP + +KC VQH S Q ++V C P+ P P C+PSLSLQ P
Sbjct: 75 MSSQLTLPAVECPEGESVKCSVQHDSNPVQELDVNCSG-PTPPPPITIPSCQPSLSLQRP 133
Query: 824 ALADLLLGSNASLTCTLSGLKKSEGVSFTWQPSGGKDAVQ 943
AL DLLLGS+AS+TCTL+GL+ EG FTW+PS GKDAVQ
Sbjct: 134 ALEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQ 173
Score = 23.1 bits (48), Expect(2) = 6e-42
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +1
Query: 985 SILPGCADPW 1014
S+LPGCA+ W
Sbjct: 188 SVLPGCAERW 197
>ref|XP_001478032.2| PREDICTED: hypothetical protein LOC100047361 [Mus musculus].
Length = 411
Score = 108 bits (271), Expect = 5e-24
Identities = 64/160 (40%), Positives = 88/160 (55%)
Frame = +2
Query: 104 LLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAYIXX 283
LL G+ + +L ESG GLV P SL ++C SGF+ + V WVRQ PGKGLEWL I
Sbjct: 253 LLPGILSQVQLKESGPGLVAPSQSLSITCTVSGFSLTGYGVNWVRQPPGKGLEWLGMI-W 311
Query: 284 XXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGGSLWGPGXXXXXXX 463
T Y ++K R +IS+DNS++ +L+MNSL+T+DTARYYC R
Sbjct: 312 GDGSTDYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTARYYCAR--------DTVREVQ 363
Query: 464 XXXTSPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVT 583
K+ G +P V+++C PS+ L+VT
Sbjct: 364 CAQCEVKLVKSKGGLVQPGRSVILSCA-----PSDSLSVT 398
Score = 77.0 bits (188), Expect = 2e-14
Identities = 40/94 (42%), Positives = 58/94 (61%)
Frame = +2
Query: 116 VQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAYIXXXXXX 295
V + ++ ESG LVQP +L L+C SGF+ SS V W R+ P KGLEWL I
Sbjct: 17 VLSQVQMKESGPDLVQPSQTLSLTCTVSGFSLSSYGVHWFRKPPRKGLEWLGGI-WSGGS 75
Query: 296 TYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 397
YY ++ R ++SRD S++ + +M+SL++EDT
Sbjct: 76 IYYNPALSSRLSVSRDISKSQVFFKMSSLQSEDT 109
>ref|XP_003086829.1| PREDICTED: similar to immunoglobulin mu-chain-like [Mus musculus].
Length = 124
Score = 103 bits (256), Expect = 3e-22
Identities = 49/114 (42%), Positives = 74/114 (64%), Gaps = 2/114 (1%)
Frame = +2
Query: 83 NWVVLFAL--LQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKG 256
+W+ LF L GV E +L +SG LV+PG S+++SC SG+TF++ + WV+Q+ GK
Sbjct: 4 SWIFLFLLSGTAGVLSEVQLHQSGPELVKPGASVKMSCKASGYTFTNYYMHWVKQSHGKS 63
Query: 257 LEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
+EW+ YI T Y KG+ T++ D S +TAY+++ SL +ED+A YYC R
Sbjct: 64 IEWIGYIYPNNGDTSYNQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCAR 117
>ref|XP_923669.1| PREDICTED: putative V-set and immunoglobulin domain-containing
protein 6-like [Mus musculus].
Length = 208
Score = 102 bits (253), Expect = 6e-22
Identities = 53/111 (47%), Positives = 71/111 (63%)
Frame = +2
Query: 86 WVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEW 265
++ L A V + +L ESG GLV P SL ++C SGF+ +S V WVRQ PGKGLEW
Sbjct: 7 FLCLVAFPSCVLSQVQLKESGPGLVAPSQSLSITCTVSGFSLTSYGVHWVRQPPGKGLEW 66
Query: 266 LAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
L I T Y ++ R +IS+DNS++ +L+MNSL+T+DTA YYC R
Sbjct: 67 LGVI-WAGGSTNYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYYCAR 116
>ref|XP_003086828.1| PREDICTED: hypothetical protein LOC100505002 [Mus musculus].
Length = 302
Score = 97.8 bits (242), Expect = 1e-20
Identities = 47/114 (41%), Positives = 72/114 (63%), Gaps = 2/114 (1%)
Frame = +2
Query: 83 NWVVLFAL--LQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKG 256
+W+ LF L GV E +L +SG L++PG S+++SC SG+TF+ + WV+Q+ GK
Sbjct: 4 SWIFLFLLSGTAGVLSEVQLQQSGPELLKPGASVKISCKASGYTFTDYTMHWVKQSHGKS 63
Query: 257 LEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
LEW+ I T Y + K + T++ D S +TAY+++ SL +ED+A YYC R
Sbjct: 64 LEWIGGINPNYGGTSYNEKFKDKATLTVDKSSSTAYMELRSLTSEDSAVYYCAR 117
Score = 72.0 bits (175), Expect = 7e-13
Identities = 37/102 (36%), Positives = 61/102 (59%)
Frame = +2
Query: 113 GVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAYIXXXXX 292
GV E L +S +V+ G S+++SC SG++F+ + WV+Q+ GK L+ + YI
Sbjct: 179 GVHSEVHLQQSLPKVVKAGPSVKISCKASGYSFTGYYMHWVKQSHGKILQRVEYINPYNG 238
Query: 293 XTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR 418
T Y + K + T++ D S +TAY+ ++SL +ED+ Y C R
Sbjct: 239 GTGYIEKFKDKATLTADKSFSTAYMHLSSLTSEDSEVYSCAR 280
Score = 56.6 bits (135), Expect = 3e-08
Identities = 25/62 (40%), Positives = 39/62 (62%)
Frame = +2
Query: 92 VLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLA 271
V + V E +L +SG LV+PG S+++SC SG++F+ + WV+Q+ GK LEW+
Sbjct: 112 VYYCARHSVNSEVQLQQSGPELVKPGASVKISCKASGYSFTGYYMHWVKQSHGKSLEWIG 171
Query: 272 YI 277
I
Sbjct: 172 EI 173
>ref|XP_001474061.2| PREDICTED: hypothetical protein LOC100045292 [Mus musculus].
Length = 419
Score = 90.9 bits (224), Expect = 1e-18
Identities = 44/107 (41%), Positives = 66/107 (61%), Gaps = 3/107 (2%)
Frame = +2
Query: 104 LLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAYIXX 283
LL G + +L +SG LV+PG S+++SC SG+TF+S + WV+Q PG+GLEW+ I
Sbjct: 4 LLAGFNSQVQLQQSGAELVRPGASVKMSCKASGYTFTSYWMHWVKQRPGQGLEWIGAIDP 63
Query: 284 XXXXTYYADSVK---GRFTISRDNSQNTAYLQMNSLRTEDTARYYCF 415
T Y K + T++ D S + AY+Q +SL +ED+ YYC+
Sbjct: 64 SDSETIYNQKFKDSRDKATLTVDKSSSIAYMQFSSLTSEDSVVYYCY 110
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 41,426,992
Number of extensions: 1326793
Number of successful extensions: 6659
Number of sequences better than 1.0e-05: 8
Number of HSP's gapped: 6569
Number of HSP's successfully gapped: 15
Length of query: 341
Length of database: 15,617,559
Length adjustment: 102
Effective length of query: 239
Effective length of database: 12,553,887
Effective search space: 3000378993
Effective search space used: 3000378993
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000319
(1023 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_998993.1| IgG heavy chain [Sus scrofa]. 256 2e-68
Alignment gi|XP_003359207.1| PREDICTED: immunoglobulin lambda-like polype... 70 2e-12
Alignment gi|XP_003359212.1| PREDICTED: ig lambda chain V-III region SH-l... 51 9e-07
>ref|NP_998993.1| IgG heavy chain [Sus scrofa].
Length = 474
Score = 256 bits (653), Expect = 2e-68
Identities = 153/309 (49%), Positives = 183/309 (59%), Gaps = 23/309 (7%)
Frame = +2
Query: 68 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAP 247
MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSL+LSCV SGF FS V WVR P
Sbjct: 1 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLKLSCVASGFIFSKYIVNWVRLVP 60
Query: 248 GKGLEWLAYIXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFR--- 418
GKGLEWLA I T+YADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA YYC R
Sbjct: 61 GKGLEWLASI-WSTGSTHYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTALYYCAREVC 119
Query: 419 ------------GGSLWGPGXXXXXXXXXXTSPKIFPLTLGSSEPAG-YVVIACLVRDFF 559
G LWGPG T+P ++PL + +G V + CL +F
Sbjct: 120 FRYNTGCWDGYYGMDLWGPGVEVVVSSAPKTAPSVYPLAPCGRDTSGPNVALGCLASSYF 179
Query: 560 PSEPLTVTWSPSREGVIVRNFPPA-QAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKS 736
P EP+T+TW+ V FP Q GLY++SS +T+P + + C V H + +
Sbjct: 180 P-EPVTMTWNSGALTSGVHTFPSVLQPSGLYSLSSMVTVPASSL-SSKSYTCNVNHPATT 237
Query: 737 SQ-SVNVPCKVLPSDP-CPQC--CKPSLSLQPPALAD-LLLGSNASLTCTLSGLKKSEG- 898
++ V K P P CP C PS+ + PP D L++ +TC + + K
Sbjct: 238 TKVDKRVGTKTKPPCPICPGCEVAGPSVFIFPPKPKDTLMISQTPEVTCVVVDVSKEHAE 297
Query: 899 VSFTWQPSG 925
V F+W G
Sbjct: 298 VQFSWYVDG 306
>ref|XP_003359207.1| PREDICTED: immunoglobulin lambda-like polypeptide 5-like [Sus
scrofa].
Length = 233
Score = 70.1 bits (170), Expect = 2e-12
Identities = 64/222 (28%), Positives = 89/222 (40%), Gaps = 12/222 (5%)
Frame = +2
Query: 95 LFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAY 274
L +L G +L + G V GG+ ++C G F S W +Q PG+ + Y
Sbjct: 10 LLSLCTGYVASSQLTQPPGVSVSLGGTASITC--QGGNFGSYYAHWYKQKPGQSPMLVIY 67
Query: 275 IXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC-----FRGGSLWGP 439
A + RF+ S+ S NTA L ++ + ED A YYC G L+G
Sbjct: 68 -----EYNKRASGIPDRFSGSK--SGNTATLTISGAQAEDEADYYCQSSDDSDAGPLFGR 120
Query: 440 GXXXXXXXXXXTSP--KIFPLTLGSSEPAG--YVVIACLVRDFFPSEPLTVTWSPSREGV 607
G +P +FP SSE G + CL+ DF+P +TVTW V
Sbjct: 121 GTRLSVLRQPKAAPTVNLFP---PSSEELGTNKATLVCLISDFYPG-AVTVTWKAGGTTV 176
Query: 608 ---IVRNFPPAQAGGLYTMSSQLTLPVEQCPADQILKCQVQH 724
+ P Q+ Y SS L L + CQV H
Sbjct: 177 TQGVETTKPSKQSNNKYAASSYLALSASDWKSSSGFTCQVTH 218
>ref|XP_003359212.1| PREDICTED: ig lambda chain V-III region SH-like [Sus scrofa].
Length = 179
Score = 51.2 bits (121), Expect = 9e-07
Identities = 49/175 (28%), Positives = 70/175 (40%), Gaps = 4/175 (2%)
Frame = +2
Query: 95 LFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVDSGFTFSSRAVRWVRQAPGKGLEWLAY 274
L +L G KL + G V GG+ ++C G F S W +Q PG+ + Y
Sbjct: 10 LLSLCTGYVASSKLTQPPGVSVSLGGTASIAC--QGDNFGSYYAHWYQQKPGQSPMLVIY 67
Query: 275 IXXXXXXTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCFRGGSLWGP-GXXX 451
A + RF+ S+ S NTA L ++ + ED A YYC + P
Sbjct: 68 -----EYNKRASGIPDRFSGSK--SGNTATLTISGAQAEDEADYYCQSSDDSYTPHSDTG 120
Query: 452 XXXXXXXTSPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSP---SREGV 607
T P++ L L E G CL+ S P+ + SP S +GV
Sbjct: 121 GGGRGTQTPPQLGHLLLQHQEACGQSKNRCLLGSTDLSSPIFPSPSPQAGSAQGV 175
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 31,307,473
Number of extensions: 1036159
Number of successful extensions: 5488
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 5423
Number of HSP's successfully gapped: 3
Length of query: 341
Length of database: 11,343,932
Length adjustment: 100
Effective length of query: 241
Effective length of database: 8,854,232
Effective search space: 2133869912
Effective search space used: 2133869912
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000319
(1023 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GL896422.1| Sus scrofa unplaced genomic scaffold chrU_scaffol... 595 e-167
gb|GL896114.1| Sus scrofa unplaced genomic scaffold chrU_scaffol... 280 1e-72
>gb|GL896422.1| Sus scrofa unplaced genomic scaffold chrU_scaffold764
Length = 7672
Score = 595 bits (300), Expect = e-167
Identities = 303/304 (99%)
Strand = Plus / Plus
Query: 461 tcagtgtctgaaaccagccccaaaatcttcccactgactctggggagcagcgagcctgcc 520
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1885 tcagtgtctgaaaccagccccaaaatcttcccactgactctggggagcagcgagcctgcc 1944
Query: 521 ggatatgtggtcatcgcctgcctggtccgggacttcttcccgtcagagcccctgacagta 580
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1945 ggatatgtggtcatcgcctgcctggtccgggacttcttcccgtcagagcccctgacagta 2004
Query: 581 acctggagccctagtcgcgagggcgttattgtcagaaacttcccccctgcgcaggccggg 640
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2005 acctggagccctagtcgcgagggcgttattgtcagaaacttcccccctgcgcaggccggg 2064
Query: 641 ggcctgtacaccatgagcagccagctgaccttgccagtcgagcagtgcccagccgatcag 700
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 2065 ggcctgtacaccatgagcagccaggtgaccttgccagtcgagcagtgcccagccgatcag 2124
Query: 701 atcctgaagtgccaagtgcagcacctctccaaatccagccagtccgtgaacgtgccctgc 760
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2125 atcctgaagtgccaagtgcagcacctctccaaatccagccagtccgtgaacgtgccctgc 2184
Query: 761 aaag 764
||||
Sbjct: 2185 aaag 2188
Score = 468 bits (236), Expect = e-129
Identities = 255/260 (98%), Gaps = 1/260 (0%)
Strand = Plus / Plus
Query: 763 agttttaccttcagatccatgtccccagtgctgcaagcccagcctgtccctgcagccacc 822
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2334 agttttaccttcagatccatgtccccagtgctgcaagcccagcctgtccctgcagccacc 2393
Query: 823 agcccttgcagacctgctcctgggctccaatgccagcctcacctgcacactcagtggcct 882
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2394 agcccttgcagacctgctcctgggctccaatgccagcctcacctgcacactcagtggcct 2453
Query: 883 gaaaaaatccgagggtgtcagcttcacctggcagccctcaggtgggaaggatgccgtcca 942
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2454 gaaaaaatccgagggtgtcagcttcacctggcagccctcaggtgggaaggatgccgtcca 2513
Query: 943 agcgtcccccacgcgtgattcctgtggctgctaaagcgggt-cagcatcctgccaggctg 1001
|||||||||||||||||||||||||||||||| |||| || ||||||||||||||||||
Sbjct: 2514 ggcgtcccccacgcgtgattcctgtggctgctacagcgtgtccagcatcctgccaggctg 2573
Query: 1002 tgctgatccttggaaaaaag 1021
||||||||||||||| ||||
Sbjct: 2574 tgctgatccttggaacaaag 2593
>gb|GL896114.1| Sus scrofa unplaced genomic scaffold chrU_scaffold487
Length = 3028
Score = 280 bits (141), Expect = 1e-72
Identities = 159/165 (96%)
Strand = Plus / Minus
Query: 248 gggaaggggctggagtggctggcatatattagtagtagtagtgaaagcacctactacgca 307
||||||||||||||||||||||||||||||| ||||||| ||| |||||||||||||||
Sbjct: 2633 gggaaggggctggagtggctggcatatattactagtagtggtggtagcacctactacgca 2574
Query: 308 gactctgtgaagggccgattcaccatctccagagacaactcccagaacacggcctatttg 367
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||| ||
Sbjct: 2573 gactctgtgaagggccgattcaccatctccagcgacaactcccagaacacggcctatctg 2514
Query: 368 caaatgaacagcctgagaaccgaagacacggcccgctattactgt 412
|||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2513 caaatgaacagcctgagaaccgaagacacggcccgctattactgt 2469
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 28,981,834
Number of extensions: 189
Number of successful extensions: 189
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 188
Number of HSP's successfully gapped: 3
Length of query: 1023
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 1002
Effective length of database: 2,808,413,156
Effective search space: 2814029982312
Effective search space used: 2814029982312
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 29 (58.0 bits)