Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002225
(1208 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001039861.1| 3,2-trans-enoyl-CoA isomerase, mitochondrial... 450 e-126
Alignment gi|NP_776760.1| trifunctional enzyme subunit alpha, mitochondri... 79 8e-15
Alignment gi|NP_001180085.1| enoyl-CoA hydratase domain-containing protei... 70 5e-12
Alignment gi|XP_002692097.1| PREDICTED: enoyl Coenzyme A hydratase domain... 70 5e-12
Alignment gi|NP_001033625.1| enoyl-CoA hydratase domain-containing protei... 62 8e-10
Alignment gi|NP_001020377.2| enoyl-CoA hydratase, mitochondrial precursor... 62 1e-09
Alignment gi|NP_001030492.1| enoyl-CoA hydratase domain-containing protei... 62 1e-09
Alignment gi|NP_001069248.2| peroxisomal bifunctional enzyme [Bos taurus]. 60 5e-09
Alignment gi|XP_002695024.1| PREDICTED: enoyl Coenzyme A hydratase 1, per... 52 1e-06
Alignment gi|XP_002701918.1| PREDICTED: enoyl Coenzyme A hydratase 1, per... 52 1e-06
>ref|NP_001039861.1| 3,2-trans-enoyl-CoA isomerase, mitochondrial [Bos taurus].
Length = 303
Score = 450 bits (1158), Expect = e-126
Identities = 224/278 (80%), Positives = 253/278 (91%), Gaps = 1/278 (0%)
Frame = +3
Query: 117 GVALGRTE-AAAGIRNGARRFGSQHVLVEPAAAAGVAVMKLRNPPVNSLSLELLTELVIS 293
G LGRTE AA G +GARRFGSQ VLVEP AA+GVAVMKLRNPPVN+LSLELLTELVIS
Sbjct: 23 GTTLGRTEPAAGGGGDGARRFGSQRVLVEPDAASGVAVMKLRNPPVNALSLELLTELVIS 82
Query: 294 LEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKAVQEMWLRFYLSNLV 473
LEKLENDK FRGVILTSDCP VF +GLDL E+CGRNPA AE+WKA+QE+WLR YLS+LV
Sbjct: 83 LEKLENDKTFRGVILTSDCPRVFCAGLDLTEICGRNPAHCAEYWKAMQELWLRTYLSSLV 142
Query: 474 LVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRA 653
LVAAINGACPAGGC+++L+CD RVLADNPKY +GLNETLLGI+APFW KD+ +NTIGHRA
Sbjct: 143 LVAAINGACPAGGCIIALSCDCRVLADNPKYRIGLNETLLGIIAPFWLKDTYVNTIGHRA 202
Query: 654 TEQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQA 833
+EQALQLGSL PPAEALQVG+VDQVVPEDQ+ STALS MA+WLAVPDHARQLTK+M+R+A
Sbjct: 203 SEQALQLGSLFPPAEALQVGLVDQVVPEDQVQSTALSEMARWLAVPDHARQLTKNMMRKA 262
Query: 834 TADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLKQ 947
TADRLL+QRDAD QNFVSF+ +D+IQKSLQ YLEKL+Q
Sbjct: 263 TADRLLRQRDADTQNFVSFVCRDSIQKSLQAYLEKLRQ 300
>ref|NP_776760.1| trifunctional enzyme subunit alpha, mitochondrial [Bos taurus].
Length = 773
Score = 79.0 bits (193), Expect = 8e-15
Identities = 52/174 (29%), Positives = 90/174 (51%), Gaps = 3/174 (1%)
Frame = +3
Query: 219 VAVMKLRNP--PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
VAV+++ +P VN+LS EL +E + + ++ + R +L S P F +G DL L
Sbjct: 59 VAVIRINSPNSKVNTLSQELHSEFMEVMNEVWSSSQIRSAVLISTKPGCFIAGADLNMLN 118
Query: 393 GRNPAR-YAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
++ + + Q+M+ + S +VAAING+C GG L+++C YR+ + K +
Sbjct: 119 SCTTSQEVTQISQEAQKMFEKLEKSTKPVVAAINGSCLGGGLELAISCQYRIATKDKKTV 178
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV 731
+G E LLGI+ + +G A + G + A ++G+VDQ+V
Sbjct: 179 LGSPEVLLGILPGAGATQRLPKMVGRPAAFDMMLTGRGIRADRAKKMGLVDQLV 232
>ref|NP_001180085.1| enoyl-CoA hydratase domain-containing protein 3, mitochondrial [Bos
taurus].
Length = 300
Score = 69.7 bits (169), Expect = 5e-12
Identities = 58/235 (24%), Positives = 107/235 (45%), Gaps = 4/235 (1%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCG-RNPARYAEFWK 428
N+LSL +L L + + + +I++++ P VFSSG DL+EL + P +AE ++
Sbjct: 68 NALSLAMLKSLQSDILHEAESQDLKVIIISAEGP-VFSSGHDLKELTDEQGPDYHAEVFQ 126
Query: 429 AVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLM-GLNETLLGIVA 605
E+ + + ++A +NG A GC L +CD V +D + M G+N +
Sbjct: 127 TCSEVMMLIQNHPVPIIAMVNGLATAAGCQLVASCDIAVASDKSSFAMPGVN------IG 180
Query: 606 PFWFKDSIM--NTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKW 779
F ++ + + + L G + EAL G++ +VVPE++L + + K
Sbjct: 181 VFCSTPAVALGRAVPRKVALEMLFTGEPISAQEALLHGLLSRVVPEERLEEETMRIARKV 240
Query: 780 LAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLK 944
++ L K+ + A L Q V + Q+ ++ +L+K K
Sbjct: 241 ASLSRSVLSLGKAAFYRQLAQDLRTAYHLTSQTMVDNLGLPDGQEGVKAFLQKRK 295
>ref|XP_002692097.1| PREDICTED: enoyl Coenzyme A hydratase domain containing 3-like [Bos
taurus].
Length = 300
Score = 69.7 bits (169), Expect = 5e-12
Identities = 58/235 (24%), Positives = 107/235 (45%), Gaps = 4/235 (1%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCG-RNPARYAEFWK 428
N+LSL +L L + + + +I++++ P VFSSG DL+EL + P +AE ++
Sbjct: 68 NALSLAMLKSLQSDILHEAESQDLKVIIISAEGP-VFSSGHDLKELTDEQGPDYHAEVFQ 126
Query: 429 AVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLM-GLNETLLGIVA 605
E+ + + ++A +NG A GC L +CD V +D + M G+N +
Sbjct: 127 TCSEVMMLIQNHPVPIIAMVNGLATAAGCQLVASCDIAVASDKSSFAMPGVN------IG 180
Query: 606 PFWFKDSIM--NTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKW 779
F ++ + + + L G + EAL G++ +VVPE++L + + K
Sbjct: 181 VFCSTPAVALGRAVPRKVALEMLFTGEPISAQEALLHGLLSRVVPEERLEEETMRIARKV 240
Query: 780 LAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLK 944
++ L K+ + A L Q V + Q+ ++ +L+K K
Sbjct: 241 ASLSRSVLSLGKAAFYRQLAQDLRTAYHLTSQTMVDNLGLPDGQEGVKAFLQKRK 295
>ref|NP_001033625.1| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
precursor [Bos taurus].
Length = 296
Score = 62.4 bits (150), Expect = 8e-10
Identities = 46/177 (25%), Positives = 85/177 (48%), Gaps = 1/177 (0%)
Frame = +3
Query: 216 GVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+A + + P N+L +++L+ +L +L D+ R +I S VF +G DL+E
Sbjct: 45 GIAEILMNRPSARNALGNVFVSQLLEALAQLREDRQVRVLIFRSGVKGVFCAGADLKERE 104
Query: 393 GRNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLM 572
+ A F + ++ + +AA++G GG L+L CD RV A + +M
Sbjct: 105 QMSEAEVGLFVQRLRGLMTEIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSA--VM 162
Query: 573 GLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ 743
GL ET G++ + +G ++ + G L A+A +G+V+ V +++
Sbjct: 163 GLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLSGAQAQALGLVNHAVAQNE 219
>ref|NP_001020377.2| enoyl-CoA hydratase, mitochondrial precursor [Bos taurus].
Length = 290
Score = 62.0 bits (149), Expect = 1e-09
Identities = 51/245 (20%), Positives = 106/245 (43%), Gaps = 1/245 (0%)
Frame = +3
Query: 213 AGVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLREL 389
+ V +++L P +N+L L+ EL +L+ E D ++LT VF++G D++E+
Sbjct: 45 SNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTGG-EKVFAAGADIKEM 103
Query: 390 CGRNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
++ + W + ++AA+NG GGC L++ CD ++ K
Sbjct: 104 --QSLTFQNCYSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCD--IIYAGEKAQ 159
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLL 749
G E L+G + + +G + + G + +A Q G+V ++ P + ++
Sbjct: 160 FGQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETVV 219
Query: 750 STALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQTY 929
A+ K + + K + A L + + + F S + + ++ + +
Sbjct: 220 EEAIQCAEKIASNSKIVTAMAKESVNAAFEMTLAEGVKLEKKLFYSTFATEDRKEGMAAF 279
Query: 930 LEKLK 944
+EK K
Sbjct: 280 VEKRK 284
>ref|NP_001030492.1| enoyl-CoA hydratase domain-containing protein 1 [Bos taurus].
Length = 306
Score = 61.6 bits (148), Expect = 1e-09
Identities = 59/228 (25%), Positives = 106/228 (46%), Gaps = 8/228 (3%)
Frame = +3
Query: 213 AGVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLREL 389
+G+ ++ L NP +N+ S ++ +L+ + +LEN +G+I+ + FSSG DL +
Sbjct: 61 SGIGILTLNNPSKMNAFSGVMMLQLLEKVIELENWTEGKGLIIRG-AKNTFSSGSDLNAV 119
Query: 390 CGRN-PARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKY 566
P +Q RF L+ VA + G GG ++ CD+R++ +
Sbjct: 120 KALGTPEDGMAVCMFMQNTLTRFMRLPLISVALVQGRALGGGAEVTTACDFRLMTTQSEI 179
Query: 567 LMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVP---E 737
E +GI+ + ++ IG R + L L +AL +GMVD ++P E
Sbjct: 180 RFVHKE--MGIIPSWGGATRLVEIIGGRQALKVLSGALKLDSEKALNIGMVDDILPSSDE 237
Query: 738 DQLLSTALSVMAKWL-AVPDHARQLTKSM--IRQATADRLLKQRDADI 872
+ L A + +++ P+ R L KS+ ++ + L QR+ DI
Sbjct: 238 TECLKEAQEWLQQFIKGPPEVIRALKKSVSSCKELCLEEAL-QRERDI 284
>ref|NP_001069248.2| peroxisomal bifunctional enzyme [Bos taurus].
Length = 723
Score = 59.7 bits (143), Expect = 5e-09
Identities = 45/176 (25%), Positives = 84/176 (47%)
Frame = +3
Query: 219 VAVMKLRNPPVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGR 398
+A+++LRNPPVN++S + + SL+K D + +++ +F +G D+RE
Sbjct: 11 LALIRLRNPPVNAISTTVARGIKESLQKAITDDTVKAIVICG-ADGIFCAGADIREF--- 66
Query: 399 NPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGL 578
+ F + ++ + +VAAI GG L+L C YR+ + + +G
Sbjct: 67 --KVHKTFDIQLGDIVDEIQRNKKPVVAAIQKLALGGGLELALGCHYRIA--HAEAQVGF 122
Query: 579 NETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQL 746
E LGI+ + +G A + G + EAL++G++D++V D +
Sbjct: 123 PEVTLGILPGARGTQLLPRLVGVPAALDLIISGRHISANEALKLGIIDKIVNSDPI 178
>ref|XP_002695024.1| PREDICTED: enoyl Coenzyme A hydratase 1, peroxisomal isoform 1 [Bos
taurus].
Length = 326
Score = 51.6 bits (122), Expect = 1e-06
Identities = 62/259 (23%), Positives = 114/259 (44%), Gaps = 14/259 (5%)
Frame = +3
Query: 204 AAAAGVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDL 380
AA V ++L P N+++ E+V+ K+ D R V++ S +FSSG+DL
Sbjct: 60 AAQKHVLHVQLNRPEKRNAMNKAFWREMVVCFNKIAEDSDCR-VVVISGAGKMFSSGIDL 118
Query: 381 RELC-------GRNPARYAEFWKAV----QEMWLRFYLSNLVLVAAINGACPAGGCLLSL 527
++ G + AR + ++ QE + ++AAI+G C GG L
Sbjct: 119 MDMASDLLQPAGDDSARISWHLHSLLTRYQETFSVIEKCPKPVIAAIHGGCIGGGVDLIT 178
Query: 528 TCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPA-EAL 704
CD R + + E +G+ A + IG+++ L + A EAL
Sbjct: 179 ACDIRYSTRDS--FFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAYTARKMMADEAL 236
Query: 705 QVGMVDQVVPE-DQLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNF 881
+ G+V ++ P+ + +L A ++ A+ + A Q TK + + +++ +
Sbjct: 237 ESGLVSRLFPDKESMLDAAFTLAAEISSKSPVAVQSTKINLIYSRDHSVMESLNYMKSWN 296
Query: 882 VSFISKDAIQKSLQTYLEK 938
+S + I KS+Q +EK
Sbjct: 297 MSMLQTKDIMKSVQAAIEK 315
>ref|XP_002701918.1| PREDICTED: enoyl Coenzyme A hydratase 1, peroxisomal isoform 1 [Bos
taurus].
Length = 326
Score = 51.6 bits (122), Expect = 1e-06
Identities = 62/259 (23%), Positives = 114/259 (44%), Gaps = 14/259 (5%)
Frame = +3
Query: 204 AAAAGVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDL 380
AA V ++L P N+++ E+V+ K+ D R V++ S +FSSG+DL
Sbjct: 60 AAQKHVLHVQLNRPEKRNAMNKAFWREMVVCFNKIAEDSDCR-VVVISGAGKMFSSGIDL 118
Query: 381 RELC-------GRNPARYAEFWKAV----QEMWLRFYLSNLVLVAAINGACPAGGCLLSL 527
++ G + AR + ++ QE + ++AAI+G C GG L
Sbjct: 119 MDMASDLLQPAGDDSARISWHLHSLLTRYQETFSVIEKCPKPVIAAIHGGCIGGGVDLIT 178
Query: 528 TCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPA-EAL 704
CD R + + E +G+ A + IG+++ L + A EAL
Sbjct: 179 ACDIRYSTRDS--FFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAYTARKMMADEAL 236
Query: 705 QVGMVDQVVPE-DQLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNF 881
+ G+V ++ P+ + +L A ++ A+ + A Q TK + + +++ +
Sbjct: 237 ESGLVSRLFPDKESMLDAAFTLAAEISSKSPVAVQSTKINLIYSRDHSVMESLNYMKSWN 296
Query: 882 VSFISKDAIQKSLQTYLEK 938
+S + I KS+Q +EK
Sbjct: 297 MSMLQTKDIMKSVQAAIEK 315
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 35,518,242
Number of extensions: 846950
Number of successful extensions: 3337
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 3319
Number of HSP's successfully gapped: 10
Length of query: 402
Length of database: 17,681,374
Length adjustment: 104
Effective length of query: 298
Effective length of database: 14,240,222
Effective search space: 4243586156
Effective search space used: 4243586156
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002225
(1208 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_547179.2| PREDICTED: similar to 3,2-trans-enoyl-CoA isome... 428 e-120
Alignment gi|XP_533549.2| PREDICTED: similar to Methylglutaconyl-CoA hydr... 75 1e-13
Alignment gi|XP_859489.1| PREDICTED: similar to hydroxyacyl dehydrogenase... 75 2e-13
Alignment gi|XP_532894.1| PREDICTED: similar to hydroxyacyl dehydrogenase... 75 2e-13
Alignment gi|XP_544259.2| PREDICTED: similar to enoyl Coenzyme A hydratas... 72 1e-12
Alignment gi|XP_537945.2| PREDICTED: similar to Enoyl-CoA hydratase, mito... 64 3e-10
Alignment gi|XP_545234.1| PREDICTED: similar to enoyl-Coenzyme A, hydrata... 62 8e-10
Alignment gi|XP_851564.1| PREDICTED: similar to enoyl Coenzyme A hydratas... 54 2e-07
Alignment gi|XP_853370.1| PREDICTED: similar to enoyl Coenzyme A hydratas... 50 3e-06
Alignment gi|XP_864011.1| PREDICTED: similar to enoyl Coenzyme A hydratas... 49 7e-06
>ref|XP_547179.2| PREDICTED: similar to 3,2-trans-enoyl-CoA isomerase, mitochondrial
precursor (Dodecenoyl-CoA delta-isomerase)
(D3,D2-enoyl-CoA isomerase) [Canis familiaris].
Length = 342
Score = 428 bits (1100), Expect = e-120
Identities = 212/275 (77%), Positives = 244/275 (88%)
Frame = +3
Query: 123 ALGRTEAAAGIRNGARRFGSQHVLVEPAAAAGVAVMKLRNPPVNSLSLELLTELVISLEK 302
A GRT AA G GAR FGS VLVEP AAG+AVMKLR PPVNSLSLELLTELVISLEK
Sbjct: 65 APGRTPAAGGGGPGARCFGSPRVLVEPDPAAGMAVMKLRKPPVNSLSLELLTELVISLEK 124
Query: 303 LENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKAVQEMWLRFYLSNLVLVA 482
LENDK F+GVI+TSD P +FS+GLDL E+CGR+PA YAE+WKAVQE+WLR YLS+LVL+
Sbjct: 125 LENDKSFQGVIITSDSPGIFSAGLDLMEMCGRDPAHYAEYWKAVQELWLRLYLSHLVLIC 184
Query: 483 AINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRATEQ 662
A+NGA PAGGCLL+L+CDYR+LADNPKY +GLNET LGIVAPFWF D+++NTIGHRA E+
Sbjct: 185 AVNGASPAGGCLLALSCDYRILADNPKYTIGLNETQLGIVAPFWFADTLVNTIGHRAAER 244
Query: 663 ALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQATAD 842
ALQLG L PPAEALQVG+VDQVVPEDQ+ STA SVMA+WLAVP HARQLTK+++R+ATAD
Sbjct: 245 ALQLGLLFPPAEALQVGIVDQVVPEDQVQSTARSVMAQWLAVPGHARQLTKNLMRKATAD 304
Query: 843 RLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLKQ 947
RLLKQRDADIQNFVSFIS+D+IQKSL Y+EKL++
Sbjct: 305 RLLKQRDADIQNFVSFISRDSIQKSLHVYIEKLQK 339
>ref|XP_533549.2| PREDICTED: similar to Methylglutaconyl-CoA hydratase, mitochondrial
precursor (AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding protein/enoyl-CoA hydratase) [Canis
familiaris].
Length = 340
Score = 75.5 bits (184), Expect = 1e-13
Identities = 63/216 (29%), Positives = 100/216 (46%), Gaps = 9/216 (4%)
Frame = +3
Query: 123 ALGRTEAAAGIRNGARRFGSQHVLVEPAAAA--------GVAVMKL-RNPPVNSLSLELL 275
A G T AA G + RR S V E G+ V+ + R N+ S L+
Sbjct: 51 ARGWTPAAGG-GSAPRRGYSSEVKTEDELRVRYLEEENRGIVVLGINRAYAKNTFSKSLV 109
Query: 276 TELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKAVQEMWLRF 455
L +++ L++DK R +I+ S+ P +F +G DL+E NP+ F ++ +
Sbjct: 110 KMLSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMNPSEVGPFVSKIRAVIDEI 169
Query: 456 YLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMN 635
+ +AAI+G GG L+L CD RV A + K MGL ET L I+ +
Sbjct: 170 ANLPVPTIAAIDGLALGGGLELALACDIRVAASSAK--MGLVETKLAIIPGGGGTQRLPR 227
Query: 636 TIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ 743
IG ++ + +L EA VG++ V+ ++Q
Sbjct: 228 AIGMSLAKELIFSARVLDGQEAKAVGLISHVLEQNQ 263
>ref|XP_859489.1| PREDICTED: similar to hydroxyacyl dehydrogenase, subunit A isoform
3 [Canis familiaris].
Length = 761
Score = 74.7 bits (182), Expect = 2e-13
Identities = 51/174 (29%), Positives = 86/174 (49%), Gaps = 3/174 (1%)
Frame = +3
Query: 219 VAVMKLRNP--PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
VAV++L +P VN+L+ EL +E + + ++ R +L S P F +G D+ L
Sbjct: 49 VAVVRLNSPNSKVNTLNKELQSEFMEVMNEIWASDQIRSAVLISTKPGCFIAGADINMLA 108
Query: 393 G-RNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
+ + Q M+ + S +VAAI+GAC GG L+++C YR+ + K +
Sbjct: 109 SCKTHEEVTRISQEGQRMFEKLEKSTKPIVAAISGACLGGGLELAISCQYRIATKDRKTV 168
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV 731
+G E LLGI+ + +G A + G + A ++G+VDQ+V
Sbjct: 169 LGAPEVLLGILPGAGGTQRLPKMVGLPAAFDMMLTGRNIRADRAKRMGLVDQLV 222
>ref|XP_532894.1| PREDICTED: similar to hydroxyacyl dehydrogenase, subunit A isoform
2 [Canis familiaris].
Length = 762
Score = 74.7 bits (182), Expect = 2e-13
Identities = 51/174 (29%), Positives = 86/174 (49%), Gaps = 3/174 (1%)
Frame = +3
Query: 219 VAVMKLRNP--PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
VAV++L +P VN+L+ EL +E + + ++ R +L S P F +G D+ L
Sbjct: 49 VAVVRLNSPNSKVNTLNKELQSEFMEVMNEIWASDQIRSAVLISTKPGCFIAGADINMLA 108
Query: 393 G-RNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
+ + Q M+ + S +VAAI+GAC GG L+++C YR+ + K +
Sbjct: 109 SCKTHEEVTRISQEGQRMFEKLEKSTKPIVAAISGACLGGGLELAISCQYRIATKDRKTV 168
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV 731
+G E LLGI+ + +G A + G + A ++G+VDQ+V
Sbjct: 169 LGAPEVLLGILPGAGGTQRLPKMVGLPAAFDMMLTGRNIRADRAKRMGLVDQLV 222
>ref|XP_544259.2| PREDICTED: similar to enoyl Coenzyme A hydratase domain containing
3 [Canis familiaris].
Length = 261
Score = 72.0 bits (175), Expect = 1e-12
Identities = 60/237 (25%), Positives = 107/237 (45%), Gaps = 6/237 (2%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCG-RNPARYAEFWK 428
N+LSL +L L L K R +I++++ P VFSSG DL+EL R+P +AE ++
Sbjct: 29 NALSLAMLKSLQTDLLHESESKDLRVIIISAEGP-VFSSGHDLKELTDERSPDYHAEVFQ 87
Query: 429 AVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLAD-----NPKYLMGLNETLL 593
E+ + + ++A +NG A GC L +CD V +D P +GL +
Sbjct: 88 TCSEVMMLIQNHPVPIIAMVNGLATAAGCQLVASCDIAVASDKSSFATPGVNIGLFCSTP 147
Query: 594 GIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMA 773
G+ ++ + + + L G + EAL G++ +VVP +QL + +
Sbjct: 148 GV--------ALGRAVPRKVALEMLFTGEPISAQEALLHGLLSKVVPAEQLEEETMRIAR 199
Query: 774 KWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLK 944
K ++ L K+ + L Q V ++ Q+ ++ +++K K
Sbjct: 200 KIASLSRRVVSLGKAAFYRQLPQDLRTAYHLTSQTMVDNVALQDGQEGIKAFIQKRK 256
>ref|XP_537945.2| PREDICTED: similar to Enoyl-CoA hydratase, mitochondrial precursor
(Short chain enoyl-CoA hydratase) (SCEH) (Enoyl-CoA
hydratase 1) [Canis familiaris].
Length = 575
Score = 63.9 bits (154), Expect = 3e-10
Identities = 53/261 (20%), Positives = 112/261 (42%), Gaps = 7/261 (2%)
Frame = +3
Query: 183 QHVLVEPAAA-AGVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPS 356
Q+++ E + V +++L P +N+L L+ EL +LE E D ++LT
Sbjct: 319 QYIITEKKGKNSNVGLIQLNRPKALNALCNGLMMELNQALEAFEKDPAVGAIVLTGG-EK 377
Query: 357 VFSSGLDLRELCGRN-----PARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLL 521
F++G D++E+ + +++ W + ++ ++AA+NG GGC L
Sbjct: 378 AFAAGADIKEMQNQTFQDCYSSKFLSHWDQLAQV-------KKPVIAAVNGYALGGGCEL 430
Query: 522 SLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEA 701
++ CD ++ K E LLG + + +G + + G + +A
Sbjct: 431 AMMCD--IIYAGEKAQFAQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDA 488
Query: 702 LQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNF 881
Q G+V ++ P + L+ A+ K + + K + A L + + + F
Sbjct: 489 KQAGLVSKIFPVETLVEEAIRCAEKIASNSKIVTAMAKESVNAAFEMTLTEGNRLEKKLF 548
Query: 882 VSFISKDAIQKSLQTYLEKLK 944
+ + + ++ + ++EK K
Sbjct: 549 YATFATEDRKEGMTAFVEKRK 569
>ref|XP_545234.1| PREDICTED: similar to enoyl-Coenzyme A, hydratase/3-hydroxyacyl
Coenzyme A dehydrogenase [Canis familiaris].
Length = 723
Score = 62.4 bits (150), Expect = 8e-10
Identities = 48/174 (27%), Positives = 84/174 (48%)
Frame = +3
Query: 219 VAVMKLRNPPVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGR 398
+A+++LRNPPVN++S +L + L+K D+ + ++L FS+G D+
Sbjct: 11 LALIRLRNPPVNAISTAVLRGIKDGLQKATTDRTVKAIVLCG-ADGKFSAGADIHSF--- 66
Query: 399 NPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGL 578
R ++F + + + +VAAI G GG L+L C YR+ + + +G
Sbjct: 67 GEPRKSDF--VLGHIVDEIQRTEKPVVAAIQGLALGGGLELALGCHYRIA--HAEAQIGF 122
Query: 579 NETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPED 740
E LGI+ + IG A + G + EAL++G++D++V D
Sbjct: 123 PEVTLGILPGARGTQLLPRLIGVPAALDLITSGRHVLADEALKLGILDEIVNSD 176
>ref|XP_851564.1| PREDICTED: similar to enoyl Coenzyme A hydratase domain containing
1 isoform 1 [Canis familiaris].
Length = 307
Score = 54.3 bits (129), Expect = 2e-07
Identities = 55/223 (24%), Positives = 102/223 (45%), Gaps = 5/223 (2%)
Frame = +3
Query: 213 AGVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLREL 389
+G+ ++ L NP +N+ S ++ +L+ + +LEN +G+I+ + FSSG DL +
Sbjct: 62 SGIGILTLNNPSKMNAFSGVMMLQLLEKVIELENWTEGKGLIVRG-AKNTFSSGSDLNAV 120
Query: 390 CG-RNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKY 566
P +Q R L+ VA I G GG ++ CD+R++ + +
Sbjct: 121 KALATPEDGMALCMFMQNTLTRLMRLPLISVALIQGRALGGGAEVTTACDFRLMTADGEI 180
Query: 567 LMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV---PE 737
E +GIV + ++ +G R + L L A AL +GMV++V+ E
Sbjct: 181 RFVHRE--MGIVPSWGGGTRLVELLGARQALKVLSGALPLDAARALSLGMVEEVLRASDE 238
Query: 738 DQLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDA 866
L A + + ++ P Q+ +++ R ++ R L +A
Sbjct: 239 ASCLQEARAWLGQFTQGPP---QVIRALKRSVSSSRELCLEEA 278
>ref|XP_853370.1| PREDICTED: similar to enoyl Coenzyme A hydratase domain containing
2 [Canis familiaris].
Length = 147
Score = 50.4 bits (119), Expect = 3e-06
Identities = 35/117 (29%), Positives = 59/117 (50%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKA 431
N+L L+ +L+ +L +L D+ R ++ S VF +G DL+E + A F +
Sbjct: 8 NALGNVLVNQLLEALAQLREDQQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGIFVQR 67
Query: 432 VQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIV 602
++ + +AA++G GG L+L CD RV A + +MGL ET G++
Sbjct: 68 LRGLMNEIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSA--VMGLIETTRGLL 122
>ref|XP_864011.1| PREDICTED: similar to enoyl Coenzyme A hydratase domain containing
1 isoform 2 [Canis familiaris].
Length = 292
Score = 49.3 bits (116), Expect = 7e-06
Identities = 53/222 (23%), Positives = 102/222 (45%), Gaps = 4/222 (1%)
Frame = +3
Query: 213 AGVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLREL 389
+G+ ++ L NP +N+ S ++ +L+ + +LEN +G+I+ + FSSG DL
Sbjct: 62 SGIGILTLNNPSKMNAFSGVMMLQLLEKVIELENWTEGKGLIVRG-AKNTFSSGSDLN-- 118
Query: 390 CGRNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
A + ++ + L+ VA I G GG ++ CD+R++ + +
Sbjct: 119 -----AVKISIFSSLNRL-------PLISVALIQGRALGGGAEVTTACDFRLMTADGEIR 166
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV---PED 740
E +GIV + ++ +G R + L L A AL +GMV++V+ E
Sbjct: 167 FVHRE--MGIVPSWGGGTRLVELLGARQALKVLSGALPLDAARALSLGMVEEVLRASDEA 224
Query: 741 QLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDA 866
L A + + ++ P Q+ +++ R ++ R L +A
Sbjct: 225 SCLQEARAWLGQFTQGPP---QVIRALKRSVSSSRELCLEEA 263
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 36,951,278
Number of extensions: 860229
Number of successful extensions: 3444
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 3426
Number of HSP's successfully gapped: 10
Length of query: 402
Length of database: 18,874,504
Length adjustment: 105
Effective length of query: 297
Effective length of database: 15,374,224
Effective search space: 4566144528
Effective search space used: 4566144528
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002225
(1208 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001910.2| enoyl-CoA delta isomerase 1, mitochondrial isof... 441 e-124
Alignment gi|NP_001171500.1| enoyl-CoA delta isomerase 1, mitochondrial i... 401 e-112
Alignment gi|NP_001689.1| methylglutaconyl-CoA hydratase, mitochondrial p... 74 3e-13
Alignment gi|NP_000173.2| trifunctional enzyme subunit alpha, mitochondri... 72 1e-12
Alignment gi|NP_001957.2| peroxisomal bifunctional enzyme isoform 1 [Homo... 62 8e-10
Alignment gi|NP_004083.3| enoyl-CoA hydratase, mitochondrial precursor [H... 62 1e-09
Alignment gi|NP_078969.2| enoyl-CoA hydratase domain-containing protein 3... 61 2e-09
Alignment gi|NP_001185890.1| enoyl-CoA hydratase domain-containing protei... 59 9e-09
Alignment gi|NP_001002030.1| enoyl-CoA hydratase domain-containing protei... 57 3e-08
Alignment gi|NP_001132982.1| enoyl-CoA hydratase domain-containing protei... 57 3e-08
>ref|NP_001910.2| enoyl-CoA delta isomerase 1, mitochondrial isoform 1 precursor
[Homo sapiens].
Length = 302
Score = 441 bits (1134), Expect = e-124
Identities = 216/277 (77%), Positives = 248/277 (89%)
Frame = +3
Query: 117 GVALGRTEAAAGIRNGARRFGSQHVLVEPAAAAGVAVMKLRNPPVNSLSLELLTELVISL 296
G ALGRTE AAG +GARRFGSQ VLVEP A AGVAVMK +NPPVNSLSLE LTELVISL
Sbjct: 23 GAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISL 82
Query: 297 EKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKAVQEMWLRFYLSNLVL 476
EKLENDK FRGVILTSD P VFS+GLDL E+CGR+PA YA +WKAVQE+WLR Y SNLVL
Sbjct: 83 EKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVL 142
Query: 477 VAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRAT 656
V+AINGACPAGGCL++LTCDYR+LADNP+Y +GLNET LGI+APFW KD++ NTIGHRA
Sbjct: 143 VSAINGACPAGGCLVALTCDYRILADNPRYCIGLNETQLGIIAPFWLKDTLENTIGHRAA 202
Query: 657 EQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQAT 836
E+ALQLG L PPAEALQVG+VDQVVPE+Q+ STALS +A+W+A+PDHARQLTK+M+R+AT
Sbjct: 203 ERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKAT 262
Query: 837 ADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLKQ 947
A RL+ QRDAD+QNFVSFISKD+IQKSLQ YLE+LK+
Sbjct: 263 ASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKE 299
>ref|NP_001171500.1| enoyl-CoA delta isomerase 1, mitochondrial isoform 2 precursor
[Homo sapiens].
Length = 285
Score = 401 bits (1030), Expect = e-112
Identities = 203/277 (73%), Positives = 233/277 (84%)
Frame = +3
Query: 117 GVALGRTEAAAGIRNGARRFGSQHVLVEPAAAAGVAVMKLRNPPVNSLSLELLTELVISL 296
G ALGRTE AAG +GARRFGSQ VLVEP A AGVAVMK +NPPVNSLSLE LTELVISL
Sbjct: 23 GAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISL 82
Query: 297 EKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKAVQEMWLRFYLSNLVL 476
EKLENDK FRGVILTSD P VFS+GLDL E+CGR+PA YA +WKAVQE+WLR Y SNLVL
Sbjct: 83 EKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVL 142
Query: 477 VAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRAT 656
V+AINGACPAGGCL++LTCDYR+LADNP+ KD++ NTIGHRA
Sbjct: 143 VSAINGACPAGGCLVALTCDYRILADNPR-----------------LKDTLENTIGHRAA 185
Query: 657 EQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQAT 836
E+ALQLG L PPAEALQVG+VDQVVPE+Q+ STALS +A+W+A+PDHARQLTK+M+R+AT
Sbjct: 186 ERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKAT 245
Query: 837 ADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLKQ 947
A RL+ QRDAD+QNFVSFISKD+IQKSLQ YLE+LK+
Sbjct: 246 ASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKE 282
>ref|NP_001689.1| methylglutaconyl-CoA hydratase, mitochondrial precursor [Homo
sapiens].
Length = 339
Score = 73.9 bits (180), Expect = 3e-13
Identities = 56/197 (28%), Positives = 94/197 (47%), Gaps = 4/197 (2%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKA 431
NSLS L+ L +++ L++DK R +I+ S+ P +F +G DL+E + + F
Sbjct: 101 NSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMSSSEVGPFVSK 160
Query: 432 VQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPF 611
++ + + +AAI+G GG L+L CD RV A + K MGL ET L I+
Sbjct: 161 IRAVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAK--MGLVETKLAIIPGG 218
Query: 612 WFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ----LLSTALSVMAKW 779
+ IG ++ + +L EA VG++ V+ ++Q AL + ++
Sbjct: 219 GGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREF 278
Query: 780 LAVPDHARQLTKSMIRQ 830
L A ++ K I Q
Sbjct: 279 LPQGPVAMRVAKLAINQ 295
>ref|NP_000173.2| trifunctional enzyme subunit alpha, mitochondrial precursor [Homo
sapiens].
Length = 763
Score = 72.0 bits (175), Expect = 1e-12
Identities = 58/222 (26%), Positives = 101/222 (45%), Gaps = 15/222 (6%)
Frame = +3
Query: 219 VAVMKLRNP--PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
VAV+++ +P VN+LS EL +E + ++ R +L S P F +G D+ L
Sbjct: 49 VAVVRINSPNSKVNTLSKELHSEFSEVMNEIWASDQIRSAVLISSKPGCFIAGADINMLA 108
Query: 393 G-RNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
+ + + Q + + S +VAAING+C GG ++++C YR+ + K +
Sbjct: 109 ACKTLQEVTQLSQEAQRIVEKLEKSTKPIVAAINGSCLGGGLEVAISCQYRIATKDRKTV 168
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV------ 731
+G E LLG + + +G A + G + A ++G+VDQ+V
Sbjct: 169 LGTPEVLLGALPGAGGTQRLPKMVGVPAALDMMLTGRSIRADRAKKMGLVDQLVEPLGPG 228
Query: 732 ---PED---QLLSTALSVMAKWLAVPDHARQLTKSMIRQATA 839
PE+ + L AK LA + + K ++ + TA
Sbjct: 229 LKPPEERTIEYLEEVAITFAKGLADKKISPKRDKGLVEKLTA 270
>ref|NP_001957.2| peroxisomal bifunctional enzyme isoform 1 [Homo sapiens].
Length = 723
Score = 62.4 bits (150), Expect = 8e-10
Identities = 50/174 (28%), Positives = 85/174 (48%)
Frame = +3
Query: 219 VAVMKLRNPPVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGR 398
+A+++LRNPPVN++S LL ++ L+K D + +++ FS+G D+R G
Sbjct: 11 LALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICG-AEGKFSAGADIR---GF 66
Query: 399 NPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGL 578
+ R F + + + +VAAI G GG L+L C YR+ + + +GL
Sbjct: 67 SAPR--TFGLTLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIA--HAEAQVGL 122
Query: 579 NETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPED 740
E LG++ + G A + G + EAL++G++D+VV D
Sbjct: 123 PEVTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVVNSD 176
>ref|NP_004083.3| enoyl-CoA hydratase, mitochondrial precursor [Homo sapiens].
Length = 290
Score = 61.6 bits (148), Expect = 1e-09
Identities = 52/243 (21%), Positives = 104/243 (42%), Gaps = 1/243 (0%)
Frame = +3
Query: 219 VAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCG 395
V +++L P +N+L L+ EL +L+ E D ++LT F++G D++E+
Sbjct: 47 VGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTGG-DKAFAAGADIKEM-- 103
Query: 396 RNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMG 575
+N + + + W ++AA+NG GGC L++ CD ++ K
Sbjct: 104 QNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCD--IIYAGEKAQFA 161
Query: 576 LNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLLST 755
E L+G + + +G + + G + +A Q G+V ++ P + L+
Sbjct: 162 QPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEE 221
Query: 756 ALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQTYLE 935
A+ K + + K + A L + + + F S + D ++ + ++E
Sbjct: 222 AIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVE 281
Query: 936 KLK 944
K K
Sbjct: 282 KRK 284
>ref|NP_078969.2| enoyl-CoA hydratase domain-containing protein 3, mitochondrial
precursor [Homo sapiens].
Length = 303
Score = 61.2 bits (147), Expect = 2e-09
Identities = 67/274 (24%), Positives = 115/274 (41%), Gaps = 3/274 (1%)
Frame = +3
Query: 132 RTEAAAGIRNGARRFGSQHVLVEPAAAAGVAVMKLRNPPV-NSLSLELLTELVISLEKLE 308
R A AG R R S L G+ + L NP N+LSL +L L +
Sbjct: 33 RDPAGAGRRESEPRPTSARQL------DGIRNIVLSNPKKRNTLSLAMLKSLQSDILHDA 86
Query: 309 NDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARY-AEFWKAVQEMWLRFYLSNLVLVAA 485
+ + +I++++ P VFSSG DL+EL Y AE ++ ++ + + ++A
Sbjct: 87 DSNDLKVIIISAEGP-VFSSGHDLKELTEEQGRDYHAEVFQTCSKVMMHIRNHPVPVIAM 145
Query: 486 INGACPAGGCLLSLTCDYRVLADNPKYLM-GLNETLLGIVAPFWFKDSIMNTIGHRATEQ 662
+NG A GC L +CD V +D + G+N L ++ + + +
Sbjct: 146 VNGLATAAGCQLVASCDIAVASDKSSFATPGVNVGLFCSTPGV----ALARAVPRKVALE 201
Query: 663 ALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQATAD 842
L G + EAL G++ +VVPE +L + + K ++ L K+ +
Sbjct: 202 MLFTGEPISAQEALLHGLLSKVVPEAELQEETMRIARKIASLSRPVVSLGKATFYKQLPQ 261
Query: 843 RLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLK 944
L Q V ++ Q+ + +L+K K
Sbjct: 262 DLGTAYYLTSQAMVDNLALRDGQEGITAFLQKRK 295
>ref|NP_001185890.1| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
isoform 1 [Homo sapiens].
Length = 292
Score = 58.9 bits (141), Expect = 9e-09
Identities = 45/177 (25%), Positives = 83/177 (46%), Gaps = 1/177 (0%)
Frame = +3
Query: 216 GVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ + + P N+L ++EL+ +L +L D+ R ++ S VF +G DL+E
Sbjct: 41 GITEILMNRPSARNALGNVFVSELLETLAQLREDRQVRVLLFRSGVKGVFCAGADLKERE 100
Query: 393 GRNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLM 572
+ A F + ++ + +AA++G GG L+L CD RV A + +M
Sbjct: 101 QMSEAEVGVFVQRLRGLMNDIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSA--VM 158
Query: 573 GLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ 743
GL ET G++ + +G ++ + G L EA +G+V+ V +++
Sbjct: 159 GLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLSGTEAHVLGLVNHAVAQNE 215
>ref|NP_001002030.1| enoyl-CoA hydratase domain-containing protein 1 isoform 1 [Homo
sapiens].
Length = 301
Score = 57.4 bits (137), Expect = 3e-08
Identities = 54/209 (25%), Positives = 92/209 (44%), Gaps = 7/209 (3%)
Frame = +3
Query: 216 GVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ ++ L NP +N+ S ++ +L+ + +LEN +G+I+ + FSSG DL +
Sbjct: 57 GIGILTLNNPSRMNAFSGVMMLQLLEKVIELENWTEGKGLIVRG-AKNTFSSGSDLNAVK 115
Query: 393 GRN-PARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
P +Q RF L+ VA + G GG + CD+R++ K
Sbjct: 116 SLGTPEDGMAVCMFMQNTLTRFMRLPLISVALVQGWALGGGAEFTTACDFRLMTPESKIR 175
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLL 749
E +GI+ + ++ IG R + L L AL +GMV++V+
Sbjct: 176 FVHKE--MGIIPSWGGTTRLVEIIGSRQALKVLSGALKLDSKNALNIGMVEEVLQSSD-E 232
Query: 750 STALSVMAKWL-----AVPDHARQLTKSM 821
+ +L +WL P+ R L KS+
Sbjct: 233 TKSLEEAQEWLKQFIQGPPEVIRALKKSV 261
>ref|NP_001132982.1| enoyl-CoA hydratase domain-containing protein 1 isoform 5 [Homo
sapiens].
Length = 307
Score = 57.4 bits (137), Expect = 3e-08
Identities = 54/209 (25%), Positives = 92/209 (44%), Gaps = 7/209 (3%)
Frame = +3
Query: 216 GVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ ++ L NP +N+ S ++ +L+ + +LEN +G+I+ + FSSG DL +
Sbjct: 63 GIGILTLNNPSRMNAFSGVMMLQLLEKVIELENWTEGKGLIVRG-AKNTFSSGSDLNAVK 121
Query: 393 GRN-PARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
P +Q RF L+ VA + G GG + CD+R++ K
Sbjct: 122 SLGTPEDGMAVCMFMQNTLTRFMRLPLISVALVQGWALGGGAEFTTACDFRLMTPESKIR 181
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLL 749
E +GI+ + ++ IG R + L L AL +GMV++V+
Sbjct: 182 FVHKE--MGIIPSWGGTTRLVEIIGSRQALKVLSGALKLDSKNALNIGMVEEVLQSSD-E 238
Query: 750 STALSVMAKWL-----AVPDHARQLTKSM 821
+ +L +WL P+ R L KS+
Sbjct: 239 TKSLEEAQEWLKQFIQGPPEVIRALKKSV 267
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 36,084,825
Number of extensions: 831472
Number of successful extensions: 3018
Number of sequences better than 1.0e-05: 11
Number of HSP's gapped: 3003
Number of HSP's successfully gapped: 11
Length of query: 402
Length of database: 18,297,164
Length adjustment: 105
Effective length of query: 297
Effective length of database: 14,835,944
Effective search space: 4406275368
Effective search space used: 4406275368
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002225
(1208 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_034153.2| enoyl-CoA delta isomerase 1, mitochondrial prec... 408 e-114
Alignment gi|NP_849209.1| trifunctional enzyme subunit alpha, mitochondri... 74 2e-13
Alignment gi|NP_057918.2| methylglutaconyl-CoA hydratase, mitochondrial p... 74 2e-13
Alignment gi|NP_444349.1| enoyl-CoA hydratase, mitochondrial precursor [M... 73 5e-13
Alignment gi|NP_077170.2| enoyl-CoA hydratase domain-containing protein 3... 66 6e-11
Alignment gi|NP_001103665.1| enoyl-CoA hydratase domain-containing protei... 62 9e-10
Alignment gi|NP_080131.4| enoyl-CoA hydratase domain-containing protein 1... 62 9e-10
Alignment gi|NP_076226.2| peroxisomal bifunctional enzyme [Mus musculus]. 62 1e-09
Alignment gi|NP_081004.2| enoyl-CoA hydratase domain-containing protein 2... 60 3e-09
Alignment gi|NP_058052.1| delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mi... 49 8e-06
>ref|NP_034153.2| enoyl-CoA delta isomerase 1, mitochondrial precursor [Mus
musculus].
Length = 289
Score = 408 bits (1048), Expect = e-114
Identities = 203/277 (73%), Positives = 234/277 (84%)
Frame = +3
Query: 117 GVALGRTEAAAGIRNGARRFGSQHVLVEPAAAAGVAVMKLRNPPVNSLSLELLTELVISL 296
G LGR EA +GARRF ++ VLVE AGVAVMKLRNPPVNSLSLE LTE ISL
Sbjct: 14 GSRLGRREAV----DGARRFANKRVLVETEGPAGVAVMKLRNPPVNSLSLECLTEFTISL 69
Query: 297 EKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKAVQEMWLRFYLSNLVL 476
EKLENDK RGVILTS+CP +FS+GLDL E+ GRNPA YAE+WK VQE+WLR Y SN++L
Sbjct: 70 EKLENDKSIRGVILTSECPGIFSAGLDLLEMYGRNPAHYAEYWKNVQELWLRLYTSNMIL 129
Query: 477 VAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRAT 656
V+AINGA PAGGCLL+L CDYRV+ADNPKY +GLNE+LLGIVAPFWFKD +NTIGHR
Sbjct: 130 VSAINGASPAGGCLLALCCDYRVMADNPKYTIGLNESLLGIVAPFWFKDMYVNTIGHREA 189
Query: 657 EQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQAT 836
E+ALQLG+L PAEAL+VG+VD+VVPEDQ+ S A SVM KWLA+PDH+RQLTK+M+R+AT
Sbjct: 190 ERALQLGTLFSPAEALKVGVVDEVVPEDQVHSKARSVMTKWLAIPDHSRQLTKNMMRKAT 249
Query: 837 ADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLKQ 947
AD L+KQR+ADIQNF SFISKD+IQKSL YLEKLKQ
Sbjct: 250 ADNLIKQREADIQNFTSFISKDSIQKSLHMYLEKLKQ 286
>ref|NP_849209.1| trifunctional enzyme subunit alpha, mitochondrial precursor [Mus
musculus].
Length = 763
Score = 74.3 bits (181), Expect = 2e-13
Identities = 58/221 (26%), Positives = 105/221 (47%), Gaps = 15/221 (6%)
Frame = +3
Query: 219 VAVMKLRNP--PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
VAV+++ +P VN+L+ E+ +E + + ++ + R +L S P F +G D+ L
Sbjct: 49 VAVIRINSPNSKVNTLNKEVQSEFIEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLS 108
Query: 393 G-RNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
P + Q M+ + S +VAAI+G+C GG L++ C YR+ + K +
Sbjct: 109 SCTTPQEATRISQEGQRMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRKTV 168
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV------ 731
+G+ E LLGI+ + +G A + G + A ++G+VDQ+V
Sbjct: 169 LGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVEPLGPG 228
Query: 732 ---PEDQLLSTALSV---MAKWLAVPDHARQLTKSMIRQAT 836
PE++ + V AK LA + + +K ++ + T
Sbjct: 229 IKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLVEKLT 269
>ref|NP_057918.2| methylglutaconyl-CoA hydratase, mitochondrial precursor [Mus
musculus].
Length = 314
Score = 73.9 bits (180), Expect = 2e-13
Identities = 56/197 (28%), Positives = 94/197 (47%), Gaps = 4/197 (2%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKA 431
N+LS LL L +++ L++DK R +I+ S+ P +F +G DL+E + + F
Sbjct: 76 NALSKNLLKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAGADLKERAKMHSSEVGPFVSK 135
Query: 432 VQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPF 611
++ + + +AAI+G GG L+L CD RV A + K MGL ET L I+
Sbjct: 136 IRSVINDIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAK--MGLVETKLAIIPGG 193
Query: 612 WFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ----LLSTALSVMAKW 779
+ IG ++ + +L EA VG++ V+ ++Q AL + ++
Sbjct: 194 GGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLISHVLEQNQEGDAAYRKALDLAREF 253
Query: 780 LAVPDHARQLTKSMIRQ 830
L A ++ K I Q
Sbjct: 254 LPQGPVAMRVAKLAINQ 270
>ref|NP_444349.1| enoyl-CoA hydratase, mitochondrial precursor [Mus musculus].
Length = 290
Score = 72.8 bits (177), Expect = 5e-13
Identities = 61/270 (22%), Positives = 117/270 (43%), Gaps = 11/270 (4%)
Frame = +3
Query: 168 RRFGS----QHVLVEPAAA-AGVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRG 329
RRF S Q+++ E + V +++L P +N+L L+ EL +LE E D
Sbjct: 25 RRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGA 84
Query: 330 VILTSDCPSVFSSGLDLRELCGRN-----PARYAEFWKAVQEMWLRFYLSNLVLVAAING 494
++LT F++G D++E+ R +++ W + + ++AA+NG
Sbjct: 85 IVLTGG-DKAFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRV-------KKPVIAAVNG 136
Query: 495 ACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQL 674
GGC L++ CD ++ K G E LLG + + +G + +
Sbjct: 137 YALGGGCELAMMCD--IIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLT 194
Query: 675 GSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLK 854
G + +A Q G+V ++ P ++L+ A+ K + + K + A L +
Sbjct: 195 GDRISAQDAKQAGLVSKIFPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTE 254
Query: 855 QRDADIQNFVSFISKDAIQKSLQTYLEKLK 944
+ + F S + D ++ + ++EK K
Sbjct: 255 GNKLEKRLFYSTFATDDRREGMTAFVEKRK 284
>ref|NP_077170.2| enoyl-CoA hydratase domain-containing protein 3, mitochondrial
precursor [Mus musculus].
Length = 300
Score = 65.9 bits (159), Expect = 6e-11
Identities = 60/246 (24%), Positives = 111/246 (45%), Gaps = 3/246 (1%)
Frame = +3
Query: 216 GVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ + L NP N+LSL +L L + + + +I++++ P VFSSG DL+EL
Sbjct: 55 GIRNIVLSNPRRRNALSLAMLKSLRSDILHEAESEDLKVIIISAEGP-VFSSGHDLKELT 113
Query: 393 GRNPARY-AEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
Y AE ++ E+ + + ++A +NG A GC L +CD V +D +
Sbjct: 114 DAQGRDYHAEVFQTCSEVMMLIRNHPVPILAMVNGLATAAGCQLVASCDIAVASDKSSFA 173
Query: 570 M-GLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQL 746
G+N L ++ + + + L G + EAL+ G++ +VVPE+QL
Sbjct: 174 TPGVNVGLFCSTPAV----ALGRAVPRKVALEMLFTGEPISAQEALRHGLISKVVPEEQL 229
Query: 747 LSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQT 926
+ + + K ++ L K+ + L Q V ++ Q+ ++
Sbjct: 230 EAETMRIAKKISSLSRSVVALGKATFYKQLPQDLRTAYFLASQAMVDNLALQDGQEGIEA 289
Query: 927 YLEKLK 944
+++K K
Sbjct: 290 FIQKRK 295
>ref|NP_001103665.1| enoyl-CoA hydratase domain-containing protein 1 isoform b [Mus
musculus].
Length = 299
Score = 62.0 bits (149), Expect = 9e-10
Identities = 58/210 (27%), Positives = 96/210 (45%), Gaps = 8/210 (3%)
Frame = +3
Query: 216 GVAVMKLRNP-PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ ++ L NP +N+ S ++ +L+ + +LEN +G+I+ + F SG DL +
Sbjct: 55 GIGILTLNNPNKMNAFSGVMMLQLLERVIELENWTEGKGLIIHG-AKNTFCSGSDLNAVK 113
Query: 393 GRN-PARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
+ P +Q RF L+ VA + G GG L+ CD+R++ +
Sbjct: 114 ALSTPESGVALSMFMQNTLTRFMRLPLISVALVQGWAMGGGAELTTACDFRLMTEESVIR 173
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV-PEDQL 746
E +GIV + ++ IG R + L L EAL +G+ D+V+ P D+
Sbjct: 174 FVHKE--MGIVPSWGGTSRLVEIIGSRQALKVLSGTLKLDSKEALNIGLTDEVLQPSDE- 230
Query: 747 LSTALSVMAKWL-----AVPDHARQLTKSM 821
+TAL +WL P R L KS+
Sbjct: 231 -TTALEQAQEWLEKFVSGPPQVIRGLKKSV 259
>ref|NP_080131.4| enoyl-CoA hydratase domain-containing protein 1 isoform a [Mus
musculus].
Length = 322
Score = 62.0 bits (149), Expect = 9e-10
Identities = 58/210 (27%), Positives = 96/210 (45%), Gaps = 8/210 (3%)
Frame = +3
Query: 216 GVAVMKLRNP-PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ ++ L NP +N+ S ++ +L+ + +LEN +G+I+ + F SG DL +
Sbjct: 78 GIGILTLNNPNKMNAFSGVMMLQLLERVIELENWTEGKGLIIHG-AKNTFCSGSDLNAVK 136
Query: 393 GRN-PARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
+ P +Q RF L+ VA + G GG L+ CD+R++ +
Sbjct: 137 ALSTPESGVALSMFMQNTLTRFMRLPLISVALVQGWAMGGGAELTTACDFRLMTEESVIR 196
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV-PEDQL 746
E +GIV + ++ IG R + L L EAL +G+ D+V+ P D+
Sbjct: 197 FVHKE--MGIVPSWGGTSRLVEIIGSRQALKVLSGTLKLDSKEALNIGLTDEVLQPSDE- 253
Query: 747 LSTALSVMAKWL-----AVPDHARQLTKSM 821
+TAL +WL P R L KS+
Sbjct: 254 -TTALEQAQEWLEKFVSGPPQVIRGLKKSV 282
>ref|NP_076226.2| peroxisomal bifunctional enzyme [Mus musculus].
Length = 718
Score = 61.6 bits (148), Expect = 1e-09
Identities = 50/189 (26%), Positives = 85/189 (44%), Gaps = 3/189 (1%)
Frame = +3
Query: 219 VAVMKLRNPPVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGR 398
+A+++L NPPVN++S ++TE+ L+K D R +++ F +G D+
Sbjct: 11 LAMIRLCNPPVNAISPTVITEVRNGLQKASLDHTVRAIVICG-ANDNFCAGADIHGFKSP 69
Query: 399 NPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGL 578
+Q +VAAI G GG L+L C YR+ N K +G
Sbjct: 70 TGLTLGSLVDEIQRY-------QKPVVAAIQGVALGGGLELALGCHYRIA--NAKARVGF 120
Query: 579 NETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV---PEDQLL 749
E +LGI+ + +G + G + EAL++G++D VV P ++ +
Sbjct: 121 PEVMLGILPGARGTQLLPRVVGVPVALDLITSGRHISTDEALKLGILDVVVKSDPVEEAI 180
Query: 750 STALSVMAK 776
A +V+ K
Sbjct: 181 KFAQTVIGK 189
>ref|NP_081004.2| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
precursor [Mus musculus].
Length = 296
Score = 60.1 bits (144), Expect = 3e-09
Identities = 43/177 (24%), Positives = 85/177 (48%), Gaps = 1/177 (0%)
Frame = +3
Query: 216 GVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ + + P N+L ++EL+ +L +L D+ R ++ S VF +G DL+E
Sbjct: 45 GITEILMNRPNARNALGNVFVSELLEALAQLREDQQVRVLLFRSAVKGVFCAGADLKERE 104
Query: 393 GRNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLM 572
+ F + ++ + + +AA++G GG L+L CD R+ A + +M
Sbjct: 105 QMSDVEVGTFVQRLRGLMSEIAAFPVPTIAAMDGFALGGGLELALACDLRIAASSA--VM 162
Query: 573 GLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ 743
GL ET G++ + +G ++ + G L A+A ++G+V+ V +++
Sbjct: 163 GLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLNGAQARELGLVNHAVAQNE 219
>ref|NP_058052.1| delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
precursor [Mus musculus].
Length = 327
Score = 48.9 bits (115), Expect = 8e-06
Identities = 57/242 (23%), Positives = 105/242 (43%), Gaps = 13/242 (5%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC-------GRNPAR 410
N+++ ELV +K+ D R V++ S +F+SG+DL ++ G + AR
Sbjct: 78 NAMNRAFWRELVECFQKISKDSDCRAVVV-SGAGKMFTSGIDLMDMASELMQPSGDDAAR 136
Query: 411 YAEFWKAVQEMWLRFYL----SNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGL 578
A + + + + + + ++AAI+G C GG L CD R + +
Sbjct: 137 IAWYLRDLISKYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLVSACDIRYCTQDA--FFQI 194
Query: 579 NETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPA-EALQVGMVDQVVPE-DQLLS 752
E +G+ A + IG+++ L + A EAL G+V +V + D +L+
Sbjct: 195 KEVDMGLAADVGTLQRLPKVIGNQSLVNELTFSARKMMADEALDSGLVSRVFQDKDAMLN 254
Query: 753 TALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQTYL 932
A ++ A + A Q +K + + + + D +S + I KS+Q +
Sbjct: 255 AAFALAADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQDIIKSVQAAM 314
Query: 933 EK 938
EK
Sbjct: 315 EK 316
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 30,292,242
Number of extensions: 683689
Number of successful extensions: 2705
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 2694
Number of HSP's successfully gapped: 10
Length of query: 402
Length of database: 15,617,559
Length adjustment: 104
Effective length of query: 298
Effective length of database: 12,493,815
Effective search space: 3723156870
Effective search space used: 3723156870
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002225
(1208 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_999127.1| trifunctional enzyme subunit alpha, mitochondri... 75 6e-14
Alignment gi|XP_003130864.2| PREDICTED: hypothetical protein LOC100513945... 75 1e-13
Alignment gi|XP_001928827.3| PREDICTED: methylglutaconyl-CoA hydratase, m... 72 5e-13
Alignment gi|NP_001177104.1| enoyl-CoA hydratase, mitochondrial [Sus scro... 72 6e-13
Alignment gi|XP_003358782.1| PREDICTED: peroxisomal bifunctional enzyme [... 61 1e-09
Alignment gi|XP_003128050.2| PREDICTED: enoyl-CoA hydratase domain-contai... 60 3e-09
Alignment gi|XP_003362049.1| PREDICTED: enoyl-CoA hydratase, mitochondria... 58 1e-08
Alignment gi|NP_001038072.1| delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase,... 56 5e-08
>ref|NP_999127.1| trifunctional enzyme subunit alpha, mitochondrial [Sus scrofa].
Length = 763
Score = 75.5 bits (184), Expect = 6e-14
Identities = 58/222 (26%), Positives = 104/222 (46%), Gaps = 15/222 (6%)
Frame = +3
Query: 219 VAVMKLRNP--PVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
VAV+++ +P VN+L EL +E + + ++ + R +L S P F +G D+ L
Sbjct: 49 VAVIRINSPNSKVNTLGQELHSEFIEVMNEVWSSSQIRSAVLISSKPGCFIAGADINMLS 108
Query: 393 GRNPAR-YAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
++ + + Q + + S +VAAING C GG L+++C YR+ + K +
Sbjct: 109 ACTTSQEVTQISQEAQRTFEKLEKSTKPIVAAINGTCLGGGLELAISCQYRIATKDKKTV 168
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV------ 731
+G E LLGI+ + +G A + G + +A ++G+VDQ+V
Sbjct: 169 LGAPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRGIRADKAKKMGLVDQLVEPLGPG 228
Query: 732 ---PED---QLLSTALSVMAKWLAVPDHARQLTKSMIRQATA 839
PE+ + L AK LA + + K ++ + T+
Sbjct: 229 LKPPEERTIEYLEEVAVTFAKGLADKKISPKRDKGLVEKLTS 270
>ref|XP_003130864.2| PREDICTED: hypothetical protein LOC100513945 [Sus scrofa].
Length = 845
Score = 74.7 bits (182), Expect = 1e-13
Identities = 63/248 (25%), Positives = 113/248 (45%), Gaps = 7/248 (2%)
Frame = +3
Query: 216 GVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC 392
G+ + L NP N+LSL +L L L + + ++++++ P VFSSG DL+EL
Sbjct: 600 GIRSIVLNNPKRRNALSLAMLKSLQSDLLHDAESRDLKVIVISAEGP-VFSSGHDLKELT 658
Query: 393 G-RNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLAD----- 554
+ P +AE ++A E+ + + ++A +NG A GC L +CD V +D
Sbjct: 659 AEQGPDYHAEVFRACSEVMMLIQNHPVPIIAMVNGLATAAGCQLVASCDIAVASDKSSFA 718
Query: 555 NPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVP 734
P +GL + G+ ++ + + + L G + EAL G++ +VVP
Sbjct: 719 TPGVTIGLFCSTPGV--------AVGRALPRKVALEMLFTGEPMSAQEALLHGLLSRVVP 770
Query: 735 EDQLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFISKDAIQK 914
E++L + + K ++ L K+ + A L Q V + Q+
Sbjct: 771 EERLEEETMRIARKVASLSRPVLSLGKAAFYRQLAQDLRTAYHLASQTMVDNLGLPDAQE 830
Query: 915 SLQTYLEK 938
+Q +L+K
Sbjct: 831 GIQAFLQK 838
>ref|XP_001928827.3| PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial [Sus
scrofa].
Length = 338
Score = 72.4 bits (176), Expect = 5e-13
Identities = 49/164 (29%), Positives = 82/164 (50%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKA 431
NSLS L+ L +++ L++DK R +I+ S+ P +F +G DL+E + + F
Sbjct: 100 NSLSKNLVKMLSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSK 159
Query: 432 VQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPF 611
++ + + +AAI+G GG L+L CD RV A + K MGL ET L I+
Sbjct: 160 LRAVINEIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAK--MGLVETKLAIIPGG 217
Query: 612 WFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ 743
+ IG ++ + +L EA VG++ V+ ++Q
Sbjct: 218 GGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLISHVLEQNQ 261
>ref|NP_001177104.1| enoyl-CoA hydratase, mitochondrial [Sus scrofa].
Length = 290
Score = 72.0 bits (175), Expect = 6e-13
Identities = 57/256 (22%), Positives = 112/256 (43%), Gaps = 2/256 (0%)
Frame = +3
Query: 183 QHVLV-EPAAAAGVAVMKLRNPP-VNSLSLELLTELVISLEKLENDKVFRGVILTSDCPS 356
+H++V + ++ V +++L P +N+L L+ EL +L+ E D ++LT
Sbjct: 34 EHIVVAKKGKSSNVGLIQLNRPKALNALCKGLILELNQALQAFEEDPAVGAIVLTGG-EK 92
Query: 357 VFSSGLDLRELCGRNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCD 536
F++G D++E+ +N + W ++AA+NG GGC L++ CD
Sbjct: 93 AFAAGADIKEM--QNQTFQDCYSGGFLSHWDHLSRVRKPVIAAVNGYALGGGCELAMMCD 150
Query: 537 YRVLADNPKYLMGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGM 716
++ K G E LLG + + +G T + + G + EA Q G+
Sbjct: 151 --IIYAGEKAQFGQPEILLGTIPGTGGTQRLTRAVGKSLTMEMVLTGDRISAQEAKQAGL 208
Query: 717 VDQVVPEDQLLSTALSVMAKWLAVPDHARQLTKSMIRQATADRLLKQRDADIQNFVSFIS 896
V ++ P + L+ A+ K + + K + A L + + + F S +
Sbjct: 209 VSKIFPVETLVEEAIQCAEKIASYSKIVTAMAKESVNAAFETSLAEGLKLEKKLFYSTFA 268
Query: 897 KDAIQKSLQTYLEKLK 944
+ ++ + ++EK K
Sbjct: 269 TEDRKEGMTAFVEKRK 284
>ref|XP_003358782.1| PREDICTED: peroxisomal bifunctional enzyme [Sus scrofa].
Length = 723
Score = 61.2 bits (147), Expect = 1e-09
Identities = 55/219 (25%), Positives = 101/219 (46%), Gaps = 5/219 (2%)
Frame = +3
Query: 219 VAVMKLRNPPVNSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGR 398
+A+++LRNPPVN+LS+ +L + L+K D + +++ +FS+G D+
Sbjct: 11 LALLRLRNPPVNALSVSVLHGIKEGLQKALTDHTVKAIVICG-TDGIFSAGADIHGFSAH 69
Query: 399 NPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGL 578
+ + ++ + +VAAI G GG L+L C YR+ + + +G
Sbjct: 70 KTSDL-----TLGDIVDKIQRYEKPVVAAIQGLALGGGLELALGCHYRIA--HAEAQVGF 122
Query: 579 NETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVV---PEDQLL 749
E LGI+ + IG A + G + EA ++G++D+VV P ++ +
Sbjct: 123 PEVTLGILPGARGTQLLPRLIGVPAALDLITSGRHISANEARKLGILDEVVNSDPVEEAI 182
Query: 750 STALSVMAKWLAVPDHARQLTKSMIRQAT--ADRLLKQR 860
A V+ + L + +S+ T ++ LLK R
Sbjct: 183 RFAQRVLDQTLESRRICNKSVQSLSNMDTIFSEALLKMR 221
>ref|XP_003128050.2| PREDICTED: enoyl-CoA hydratase domain-containing protein 2,
mitochondrial-like isoform 1 [Sus scrofa].
Length = 275
Score = 59.7 bits (143), Expect = 3e-09
Identities = 45/178 (25%), Positives = 84/178 (47%), Gaps = 1/178 (0%)
Frame = +3
Query: 213 AGVAVMKLRNPPV-NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLREL 389
AG+ + + P N+L +++L+ +L +L D+ R +I S VF +G DL+E
Sbjct: 23 AGITEILMNRPSARNALGNVFVSQLLEALAQLREDRHVRVLIFRSGVKGVFCAGADLKER 82
Query: 390 CGRNPARYAEFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYL 569
+ A F + ++ + +AA++G GG L+L CD RV A + +
Sbjct: 83 EQMSEAEVGVFVQRLRGLMNEIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSA--V 140
Query: 570 MGLNETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQ 743
MGL ET G++ + +G ++ + G L +A +G+V+ V +++
Sbjct: 141 MGLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLSGMQAQALGLVNHAVAQNE 198
>ref|XP_003362049.1| PREDICTED: enoyl-CoA hydratase, mitochondrial-like [Sus scrofa].
Length = 341
Score = 57.8 bits (138), Expect = 1e-08
Identities = 48/225 (21%), Positives = 91/225 (40%)
Frame = +3
Query: 270 LLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELCGRNPARYAEFWKAVQEMWL 449
L+ EL +L+ E D ++LT F++G D++E+ +N + W
Sbjct: 116 LILELNQALQAFEEDPAVGAIVLTGG-EKAFAAGADIKEM--QNQTFQDCYSGGFLSHWD 172
Query: 450 RFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGLNETLLGIVAPFWFKDSI 629
++AA+NG GGC L++ CD ++ K G E L +
Sbjct: 173 HLSRVRKPVIAAVNGYALGGGCELAMMCD--IIYAGEKAQFGQPELLSETCPRTGGTQRL 230
Query: 630 MNTIGHRATEQALQLGSLLPPAEALQVGMVDQVVPEDQLLSTALSVMAKWLAVPDHARQL 809
+G T + + G + EA Q G+V ++ P + L+ A+ K + +
Sbjct: 231 TRAVGKSLTMEMVLTGDRISAQEAKQAGLVSKIFPVETLVEEAIQCAEKIASYSKIVTAM 290
Query: 810 TKSMIRQATADRLLKQRDADIQNFVSFISKDAIQKSLQTYLEKLK 944
K + A L + + + F S + + ++ + ++EK K
Sbjct: 291 AKESVNAAFETSLAEGLKLEKKLFYSTFATEDRKEGMTAFVEKRK 335
>ref|NP_001038072.1| delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Sus
scrofa].
Length = 322
Score = 55.8 bits (133), Expect = 5e-08
Identities = 63/251 (25%), Positives = 109/251 (43%), Gaps = 22/251 (8%)
Frame = +3
Query: 252 NSLSLELLTELVISLEKLENDKVFRGVILTSDCPSVFSSGLDLRELC-------GRNPAR 410
N+++ E+V+ K+ D R V++ S +F+SG+DL ++ G + AR
Sbjct: 73 NAMNRAFWREMVVCFNKIAQDPDCRAVVI-SGAGKMFTSGIDLVDMASDIFQPQGEDVAR 131
Query: 411 YA----EFWKAVQEMWLRFYLSNLVLVAAINGACPAGGCLLSLTCDYRVLADNPKYLMGL 578
+ QE + ++AAI+G C GG L CD R A + +
Sbjct: 132 ISWHLHNLISKYQETFSVIEKCPKPVIAAIHGGCIGGGVDLITACDIRYCAQDA--FFQV 189
Query: 579 NETLLGIVAPFWFKDSIMNTIGHRATEQALQLGSLLPPA-EALQVGMVDQVVPEDQLLST 755
E +G+ A + IG+++ L + A EAL G+V ++ P+ ++L
Sbjct: 190 KEVDIGLAADVGTLQRLPKIIGNQSLVNELAFTARKMMADEALNSGLVSRIFPDQEVLLE 249
Query: 756 ALSVMAKWLAVPDHARQLTKSMIR-QATADRLLKQRDADIQNFVSFI---------SKDA 905
A +A +A +KS + Q T LL RD + ++++ +KD
Sbjct: 250 AALALAAEIA--------SKSPVAVQGTKINLLYSRDHSVAEGLNYMKSWNMSMLQTKDV 301
Query: 906 IQKSLQTYLEK 938
I KS+Q +EK
Sbjct: 302 I-KSVQAAMEK 311
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 23,167,744
Number of extensions: 572818
Number of successful extensions: 2260
Number of sequences better than 1.0e-05: 8
Number of HSP's gapped: 2250
Number of HSP's successfully gapped: 8
Length of query: 402
Length of database: 11,343,932
Length adjustment: 101
Effective length of query: 301
Effective length of database: 8,829,335
Effective search space: 2657629835
Effective search space used: 2657629835
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002225
(1208 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_Chr03 357 9e-96
>Sscrofa_Chr03
|| Length = 144787322
Score = 357 bits (180), Expect = 9e-96
Identities = 180/180 (100%)
Strand = Plus / Minus
Query: 613 ggttcaaagacagcatcatgaacaccatcgggcatcgcgccactgagcaggccttacagc 672
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42424651 ggttcaaagacagcatcatgaacaccatcgggcatcgcgccactgagcaggccttacagc 42424592
Query: 673 tggggtcgctcttgccaccagcagaggccctccaggtgggcatggtggaccaggtggtgc 732
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42424591 tggggtcgctcttgccaccagcagaggccctccaggtgggcatggtggaccaggtggtgc 42424532
Query: 733 ctgaggaccagctcctgagcaccgcgctctcggtgatggccaagtggctggctgttccag 792
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42424531 ctgaggaccagctcctgagcaccgcgctctcggtgatggccaagtggctggctgttccag 42424472
Score = 349 bits (176), Expect = 2e-93
Identities = 225/241 (93%), Gaps = 6/241 (2%)
Strand = Plus / Minus
Query: 791 agatcacgcgcggcagctaaccaagagcatgatccgccaggccacggcagaccgcctgct 850
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42422932 agatcacgcgcggcagctaaccaagagcatgatccgccaggccacggcagaccgcctgct 42422873
Query: 851 gaagcagcgcgacgccgacatccagaacttcgtcagcttcatctccaaagacgccatcca 910
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42422872 gaagcagcgcgacgccgacatccagaacttcgtcagcttcatctccaaagacgccatcca 42422813
Query: 911 aaagtccctgcagacgtacttagaaaagctcaaacnnnnnnnnggctaagggcccggtgg 970
||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct: 42422812 aaagtccctgcagacgtacttagaaaagctcaaacaaaagaaaggctaagggcccggtgg 42422753
Query: 971 cccctgca-ccttttggggggccctctggtggtccccaagggatctaaaataaggtactt 1029
|||||||| |||| ||||||||||| |||| |||||||||||| |||||||||||||
Sbjct: 42422752 cccctgcacccttctggggggccctgtggt----cccaagggatct-aaataaggtactt 42422698
Query: 1030 t 1030
|
Sbjct: 42422697 t 42422697
Score = 295 bits (149), Expect = 3e-77
Identities = 149/149 (100%)
Strand = Plus / Minus
Query: 344 ggactgccccagtgtcttctccagcggcctggacctgagggagctgtgcgggaggaaccc 403
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42425577 ggactgccccagtgtcttctccagcggcctggacctgagggagctgtgcgggaggaaccc 42425518
Query: 404 ggcccgctacgccgagttctggaaggccgtgcaggagatgtggttgcggttctacctgtc 463
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42425517 ggcccgctacgccgagttctggaaggccgtgcaggagatgtggttgcggttctacctgtc 42425458
Query: 464 caacctggtgctggtcgccgccatcaacg 492
|||||||||||||||||||||||||||||
Sbjct: 42425457 caacctggtgctggtcgccgccatcaacg 42425429
Score = 246 bits (124), Expect = 2e-62
Identities = 124/124 (100%)
Strand = Plus / Minus
Query: 492 ggagcctgcccggctgggggctgcctcctctccctcacctgcgactaccgggtcctggct 551
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42424841 ggagcctgcccggctgggggctgcctcctctccctcacctgcgactaccgggtcctggct 42424782
Query: 552 gacaaccccaagtacctcatggggctgaatgagaccctgctgggcattgtcgcccccttc 611
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 42424781 gacaaccccaagtacctcatggggctgaatgagaccctgctgggcattgtcgcccccttc 42424722
Query: 612 tggt 615
||||
Sbjct: 42424721 tggt 42424718
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 23,685,507
Number of extensions: 153
Number of successful extensions: 153
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 153
Number of HSP's successfully gapped: 5
Length of query: 1208
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 1187
Effective length of database: 2,808,413,156
Effective search space: 3333586416172
Effective search space used: 3333586416172
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)