Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002782
(1711 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_777069.1| isocitrate dehydrogenase [NAD] subunit alpha, m... 724 0.0
Alignment gi|NP_001161346.1| isocitrate dehydrogenase [NAD] subunit beta,... 289 6e-78
Alignment gi|NP_001139344.1| isocitrate dehydrogenase [NAD] subunit beta,... 281 1e-75
Alignment gi|NP_001069781.1| isocitrate dehydrogenase [NAD] subunit gamma... 276 5e-74
Alignment gi|XP_002683942.1| PREDICTED: isocitrate dehydrogenase-like, pa... 96 7e-20
>ref|NP_777069.1| isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor
[Bos taurus].
Length = 366
Score = 724 bits (1870), Expect = 0.0
Identities = 359/363 (98%), Positives = 360/363 (99%)
Frame = +2
Query: 50 PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 229
PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW
Sbjct: 4 PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 63
Query: 230 EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 409
EERNV AIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN
Sbjct: 64 EERNVAAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 123
Query: 410 VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA 589
VRPCVSIEGYKTPY DVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITE ASKRIAEFA
Sbjct: 124 VRPCVSIEGYKTPYHDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEAASKRIAEFA 183
Query: 590 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP 769
FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP
Sbjct: 184 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP 243
Query: 770 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 949
SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN
Sbjct: 244 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 303
Query: 950 PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGN+KCSDFTEEICRRVK
Sbjct: 304 PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNSKCSDFTEEICRRVK 363
Query: 1130 DLD 1138
DLD
Sbjct: 364 DLD 366
>ref|NP_001161346.1| isocitrate dehydrogenase [NAD] subunit beta, mitochondrial isoform 1
precursor [Bos taurus].
Length = 383
Score = 289 bits (739), Expect = 6e-78
Identities = 156/357 (43%), Positives = 223/357 (62%), Gaps = 3/357 (0%)
Frame = +2
Query: 53 AWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWE 232
AW S + + ++ Q G VT++PGDG+GPE+ AV ++F AA P++++
Sbjct: 22 AWRSLCTSTVAQASSRTQGEDVRVEGAFPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQ 81
Query: 233 ERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIA--AGHPSMNLLLRKTFDLYA 406
E +++ +Q + + + SM +NK+ + G + TP+ S ++ LR+ DL+A
Sbjct: 82 EHHLSEVQNMASEEKLE-QVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFA 140
Query: 407 NVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEF 586
NV S+ GYKT + ++++V IRE TEGEYS +EH GV++ +K++T S+RIA+F
Sbjct: 141 NVVHVKSLPGYKTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKF 200
Query: 587 AFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQD 766
AF+YA R VTAVHKANIM++ DGLFLQ C EVAE IKF +M +D C+ +VQ+
Sbjct: 201 AFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFEKMIIDNCCMQLVQN 260
Query: 767 PSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFES-VHGTAPDIAGKDM 943
P QFDVLVMPNLYG+I+ +L AGL+GG GV P + A A+FE+ G+++
Sbjct: 261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAE-YAVFETGARHPFAQAVGRNI 319
Query: 944 ANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
ANPTA+LLSA MLRH+ L H+ I A IK GK T D+GG A C DFTE +
Sbjct: 320 ANPTAMLLSASNMLRHLNLEHHSNMIAEAVKKVIKVGKVRTSDMGGYATCQDFTEAV 376
>ref|NP_001139344.1| isocitrate dehydrogenase [NAD] subunit beta, mitochondrial isoform 2
precursor [Bos taurus].
Length = 385
Score = 281 bits (720), Expect = 1e-75
Identities = 152/357 (42%), Positives = 223/357 (62%), Gaps = 3/357 (0%)
Frame = +2
Query: 53 AWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWE 232
AW S + + ++ Q G VT++PGDG+GPE+ AV ++F AA P++++
Sbjct: 22 AWRSLCTSTVAQASSRTQGEDVRVEGAFPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQ 81
Query: 233 ERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIA--AGHPSMNLLLRKTFDLYA 406
E +++ +Q + + + SM +NK+ + G + TP+ S ++ LR+ DL+A
Sbjct: 82 EHHLSEVQNMASEEKLE-QVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFA 140
Query: 407 NVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEF 586
NV S+ GYKT + ++++V IRE TEGEYS +EH GV++ +K++T S+RIA+F
Sbjct: 141 NVVHVKSLPGYKTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKF 200
Query: 587 AFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQD 766
AF+YA R VTAVHKANIM++ DGLFLQ C EVAE IKF +M +D C+ +VQ+
Sbjct: 201 AFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFEKMIIDNCCMQLVQN 260
Query: 767 PSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFES-VHGTAPDIAGKDM 943
P QFDVLVMPNLYG+I+ +L AGL+GG GV P + A A+FE+ G+++
Sbjct: 261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAE-YAVFETGARHPFAQAVGRNI 319
Query: 944 ANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
ANPTA+LLSA MLRH+ L H+ I A IK GK T+D+GG + +DF + +
Sbjct: 320 ANPTAMLLSASNMLRHLNLEHHSNMIAEAVKKVIKVGKVRTRDMGGYSTTTDFIKSV 376
>ref|NP_001069781.1| isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial precursor
[Bos taurus].
Length = 392
Score = 276 bits (705), Expect = 5e-74
Identities = 150/338 (44%), Positives = 214/338 (63%), Gaps = 3/338 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 51 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSTADEEDI----RNAIMAI 106
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + D++I+ +R
Sbjct: 107 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVR 166
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ + R VTAVHKANIM++
Sbjct: 167 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFQLAQESGRKKVTAVHKANIMKL 226
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ CREVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 227 GDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 286
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 287 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 345
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
I A A++ + T D+GG S+ ++I R ++
Sbjct: 346 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHIR 383
>ref|XP_002683942.1| PREDICTED: isocitrate dehydrogenase-like, partial [Bos taurus].
Length = 306
Score = 96.3 bits (238), Expect = 7e-20
Identities = 68/215 (31%), Positives = 106/215 (49%), Gaps = 37/215 (17%)
Frame = +2
Query: 140 VTLIPGDGIGPEISAAVMKIFDAA-------KAPIQWEE----RNVTAIQGPGGKWMIPP 286
V I GDG G +I+ ++K+ DAA K I W E T + GP W+ P
Sbjct: 31 VPFIEGDGTGADITPVMIKVVDAAVQKAYGGKRKIYWMEVYAGEKATKVYGPD-VWL-PE 88
Query: 287 EAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTD--- 457
E + + + +KGPL TP+ G S+N+ LR+ DLY +RP +G +P +
Sbjct: 89 ETLDVVKDYVVSIKGPLTTPVGGGIRSLNVALRQQLDLYVCLRPVRYFKGVPSPVREPEK 148
Query: 458 VNIVTIRENTEGEYSGIEHVIVD--------------GVVQ---------SIKLITEGAS 568
++V REN+E Y+GIE++ GV + IK ++ +
Sbjct: 149 TDMVIFRENSEDIYAGIEYMAESEQAKELIQFLQTKLGVKKIRFPATSSIGIKPVSREGT 208
Query: 569 KRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLF 673
+R+ A +YA +N R++VT VHK NIM+ ++G F
Sbjct: 209 ERLVRKALQYAIDNDRASVTLVHKGNIMKFTEGGF 243
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 63,234,810
Number of extensions: 1677882
Number of successful extensions: 5384
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 5369
Number of HSP's successfully gapped: 5
Length of query: 570
Length of database: 17,681,374
Length adjustment: 108
Effective length of query: 462
Effective length of database: 14,107,870
Effective search space: 6517835940
Effective search space used: 6517835940
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002782
(1711 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_536213.2| PREDICTED: similar to isocitrate dehydrogenase ... 720 0.0
Alignment gi|XP_861257.1| PREDICTED: similar to isocitrate dehydrogenase ... 631 0.0
Alignment gi|XP_861337.1| PREDICTED: similar to isocitrate dehydrogenase ... 508 e-144
Alignment gi|XP_861581.1| PREDICTED: similar to isocitrate dehydrogenase ... 287 2e-77
Alignment gi|XP_861552.1| PREDICTED: similar to isocitrate dehydrogenase ... 287 2e-77
Alignment gi|XP_534367.2| PREDICTED: similar to isocitrate dehydrogenase ... 280 4e-75
Alignment gi|XP_867343.1| PREDICTED: similar to Isocitrate dehydrogenase ... 273 3e-73
Alignment gi|XP_538201.2| PREDICTED: similar to Isocitrate dehydrogenase ... 273 3e-73
Alignment gi|XP_867331.1| PREDICTED: similar to Isocitrate dehydrogenase ... 265 1e-70
Alignment gi|XP_542534.2| PREDICTED: similar to Isocitrate dehydrogenase ... 262 7e-70
>ref|XP_536213.2| PREDICTED: similar to isocitrate dehydrogenase 3 (NAD+) alpha isoform
1 [Canis familiaris].
Length = 366
Score = 720 bits (1858), Expect = 0.0
Identities = 355/363 (97%), Positives = 360/363 (99%)
Frame = +2
Query: 50 PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 229
PAWISKVSRLLGAFHNQKQVTR FAGGV+TVTLIPGDGIGPEISAAVMKIFDAAKAPIQW
Sbjct: 4 PAWISKVSRLLGAFHNQKQVTRAFAGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 63
Query: 230 EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 409
EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN
Sbjct: 64 EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 123
Query: 410 VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA 589
VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITE AS+RIAEFA
Sbjct: 124 VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEFA 183
Query: 590 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP 769
FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP
Sbjct: 184 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP 243
Query: 770 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 949
SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN
Sbjct: 244 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 303
Query: 950 PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
PTALLLSAVMMLRHMGLFDHAA++E ACFATIKDGKSLTKDLGGNAKCSDFTEEICRRV+
Sbjct: 304 PTALLLSAVMMLRHMGLFDHAARVEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVR 363
Query: 1130 DLD 1138
DLD
Sbjct: 364 DLD 366
>ref|XP_861257.1| PREDICTED: similar to isocitrate dehydrogenase 3 (NAD+) alpha isoform
2 [Canis familiaris].
Length = 316
Score = 631 bits (1627), Expect = 0.0
Identities = 310/316 (98%), Positives = 314/316 (99%)
Frame = +2
Query: 191 MKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSM 370
MKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSM
Sbjct: 1 MKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSM 60
Query: 371 NLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKL 550
NLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKL
Sbjct: 61 NLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKL 120
Query: 551 ITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEM 730
ITE AS+RIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEM
Sbjct: 121 ITEEASRRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEM 180
Query: 731 YLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVH 910
YLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVH
Sbjct: 181 YLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVH 240
Query: 911 GTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAK 1090
GTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA++E ACFATIKDGKSLTKDLGGNAK
Sbjct: 241 GTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARVEAACFATIKDGKSLTKDLGGNAK 300
Query: 1091 CSDFTEEICRRVKDLD 1138
CSDFTEEICRRV+DLD
Sbjct: 301 CSDFTEEICRRVRDLD 316
>ref|XP_861337.1| PREDICTED: similar to isocitrate dehydrogenase 3 (NAD+) alpha isoform
4 [Canis familiaris].
Length = 257
Score = 508 bits (1309), Expect = e-144
Identities = 251/257 (97%), Positives = 255/257 (99%)
Frame = +2
Query: 368 MNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIK 547
MNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIK
Sbjct: 1 MNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIK 60
Query: 548 LITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNE 727
LITE AS+RIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNE
Sbjct: 61 LITEEASRRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNE 120
Query: 728 MYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESV 907
MYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESV
Sbjct: 121 MYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESV 180
Query: 908 HGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNA 1087
HGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA++E ACFATIKDGKSLTKDLGGNA
Sbjct: 181 HGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARVEAACFATIKDGKSLTKDLGGNA 240
Query: 1088 KCSDFTEEICRRVKDLD 1138
KCSDFTEEICRRV+DLD
Sbjct: 241 KCSDFTEEICRRVRDLD 257
>ref|XP_861581.1| PREDICTED: similar to isocitrate dehydrogenase 3, beta subunit
isoform b precursor isoform 6 [Canis familiaris].
Length = 392
Score = 287 bits (734), Expect = 2e-77
Identities = 152/332 (45%), Positives = 214/332 (64%), Gaps = 3/332 (0%)
Frame = +2
Query: 128 GVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMD 307
G VT++PGDG+GPE+ AV ++F AA P++++E +++ +Q + + + SM
Sbjct: 56 GAFPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQEHHLSEVQNMASEEKLE-QVLSSMK 114
Query: 308 KNKMGLKGPLKTPIA--AGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRE 481
+NK+ + G + TP+ S ++ LR+ DL+ANV S+ GYKT + ++++V IRE
Sbjct: 115 ENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIRE 174
Query: 482 NTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 661
TEGEYS +EH GV++ +K++T S+RIA+FAF+YA R VTAVHKANIM++
Sbjct: 175 QTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLG 234
Query: 662 DGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 841
DGLFLQ C EVAE IKF M +D C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+
Sbjct: 235 DGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLV 294
Query: 842 GGLGVTPSGNIGANGVAIFES-VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAK 1018
GG GV P + A A+FE+ G+++ANPTA+LLSA MLRH+ L H+
Sbjct: 295 GGAGVVPGESYSAE-YAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSNM 353
Query: 1019 IETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
I A IK GK T D+GG A C DFTE +
Sbjct: 354 IADAVKKVIKVGKVRTSDMGGYATCQDFTEAV 385
>ref|XP_861552.1| PREDICTED: similar to isocitrate dehydrogenase 3, beta subunit
isoform b precursor isoform 5 [Canis familiaris].
Length = 383
Score = 287 bits (734), Expect = 2e-77
Identities = 152/332 (45%), Positives = 214/332 (64%), Gaps = 3/332 (0%)
Frame = +2
Query: 128 GVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMD 307
G VT++PGDG+GPE+ AV ++F AA P++++E +++ +Q + + + SM
Sbjct: 47 GAFPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQEHHLSEVQNMASEEKLE-QVLSSMK 105
Query: 308 KNKMGLKGPLKTPIA--AGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRE 481
+NK+ + G + TP+ S ++ LR+ DL+ANV S+ GYKT + ++++V IRE
Sbjct: 106 ENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIRE 165
Query: 482 NTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 661
TEGEYS +EH GV++ +K++T S+RIA+FAF+YA R VTAVHKANIM++
Sbjct: 166 QTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLG 225
Query: 662 DGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 841
DGLFLQ C EVAE IKF M +D C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+
Sbjct: 226 DGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLV 285
Query: 842 GGLGVTPSGNIGANGVAIFES-VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAK 1018
GG GV P + A A+FE+ G+++ANPTA+LLSA MLRH+ L H+
Sbjct: 286 GGAGVVPGESYSAE-YAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSNM 344
Query: 1019 IETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
I A IK GK T D+GG A C DFTE +
Sbjct: 345 IADAVKKVIKVGKVRTSDMGGYATCQDFTEAV 376
>ref|XP_534367.2| PREDICTED: similar to isocitrate dehydrogenase 3, beta subunit
isoform a precursor isoform 1 [Canis familiaris].
Length = 385
Score = 280 bits (715), Expect = 4e-75
Identities = 148/332 (44%), Positives = 214/332 (64%), Gaps = 3/332 (0%)
Frame = +2
Query: 128 GVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMD 307
G VT++PGDG+GPE+ AV ++F AA P++++E +++ +Q + + + SM
Sbjct: 47 GAFPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQEHHLSEVQNMASEEKLE-QVLSSMK 105
Query: 308 KNKMGLKGPLKTPIA--AGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRE 481
+NK+ + G + TP+ S ++ LR+ DL+ANV S+ GYKT + ++++V IRE
Sbjct: 106 ENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIRE 165
Query: 482 NTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 661
TEGEYS +EH GV++ +K++T S+RIA+FAF+YA R VTAVHKANIM++
Sbjct: 166 QTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLG 225
Query: 662 DGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 841
DGLFLQ C EVAE IKF M +D C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+
Sbjct: 226 DGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLV 285
Query: 842 GGLGVTPSGNIGANGVAIFES-VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAK 1018
GG GV P + A A+FE+ G+++ANPTA+LLSA MLRH+ L H+
Sbjct: 286 GGAGVVPGESYSAE-YAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSNM 344
Query: 1019 IETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
I A IK GK T+D+GG + +DF + +
Sbjct: 345 IADAVKKVIKVGKVRTRDMGGYSTTTDFIKSV 376
>ref|XP_867343.1| PREDICTED: similar to Isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial precursor (Isocitric dehydrogenase)
(NAD(+)-specific ICDH) isoform 7 [Canis familiaris].
Length = 352
Score = 273 bits (699), Expect = 3e-73
Identities = 149/338 (44%), Positives = 214/338 (63%), Gaps = 3/338 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 11 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAI 66
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + D++I+ +R
Sbjct: 67 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVR 126
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ + R VTAVHKANIM++
Sbjct: 127 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKL 186
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ C+EVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 187 GDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 246
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 247 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 305
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
I A A++ + T D+GG S+ ++I R ++
Sbjct: 306 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHIR 343
>ref|XP_538201.2| PREDICTED: similar to Isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial precursor (Isocitric dehydrogenase)
(NAD(+)-specific ICDH) isoform 1 [Canis familiaris].
Length = 392
Score = 273 bits (699), Expect = 3e-73
Identities = 149/338 (44%), Positives = 214/338 (63%), Gaps = 3/338 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 51 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAI 106
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + D++I+ +R
Sbjct: 107 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVR 166
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ + R VTAVHKANIM++
Sbjct: 167 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKL 226
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ C+EVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 227 GDGLFLQCCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 286
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 287 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 345
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
I A A++ + T D+GG S+ ++I R ++
Sbjct: 346 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHIR 383
>ref|XP_867331.1| PREDICTED: similar to Isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial precursor (Isocitric dehydrogenase)
(NAD(+)-specific ICDH) isoform 6 [Canis familiaris].
Length = 335
Score = 265 bits (677), Expect = 1e-70
Identities = 144/331 (43%), Positives = 209/331 (63%), Gaps = 3/331 (0%)
Frame = +2
Query: 146 LIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGL 325
+IPGDGIGPE+ V +F A P+ +EE +V++ A ++ +N++ L
Sbjct: 1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVAL 56
Query: 326 KGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEY 499
KG ++T + H S N +LR + DLYANV C S+ G T + D++I+ +RENTEGEY
Sbjct: 57 KGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDIDILIVRENTEGEY 116
Query: 500 SGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQ 679
S +EH V GVV+S+K+IT+ S RIAE+AF+ A+ + R VTAVHKANIM++ DGLFLQ
Sbjct: 117 SSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ 176
Query: 680 KCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVT 859
C+EVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL+GG G+
Sbjct: 177 CCKEVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 236
Query: 860 PSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACF 1036
N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A I A
Sbjct: 237 AGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVL 295
Query: 1037 ATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
A++ + T D+GG S+ ++I R ++
Sbjct: 296 ASMDNENMHTPDIGGQGTTSEAIQDIIRHIR 326
>ref|XP_542534.2| PREDICTED: similar to Isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial precursor (Isocitric dehydrogenase)
(NAD(+)-specific ICDH) [Canis familiaris].
Length = 389
Score = 262 bits (670), Expect = 7e-70
Identities = 141/334 (42%), Positives = 205/334 (61%), Gaps = 4/334 (1%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEER--NVTAIQGPGGKWMIPPEAKE 298
GG TVT+IPGDGIGPE+ V +F A P+ +EE N T+ + A
Sbjct: 54 GGRHTVTMIPGDGIGPELMLHVKTVFRHACVPVDFEEVVVNCTSCEED------IHNAIM 107
Query: 299 SMDKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVT 472
++ +N++ LKG L+T + H N + R T DLYANV S+ G +T + DV+I+
Sbjct: 108 AVRRNRVALKGNLETDHNLPPSHKCRNNMFRTTLDLYANVIHFKSLPGVETQHKDVDILV 167
Query: 473 IRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIM 652
+RENTEGEYS +EH V GV++S+K+IT+ S RIA++AF+ A+ R VT VHKANIM
Sbjct: 168 VRENTEGEYSNLEHESVTGVIESLKIITKAKSFRIAQYAFQLAQEMERKKVTVVHKANIM 227
Query: 653 RMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCA 832
++ DGLFLQ CREVA + + F M +D + +V P QFDV+VMPNLYG+I++ +C
Sbjct: 228 KLGDGLFLQCCREVASHYPQLTFEGMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNSICT 287
Query: 833 GLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHA 1012
GL+GG G+ P N G + +A ++A K++ANPTA+LL++ +ML ++ L +A
Sbjct: 288 GLVGGAGLVPGANYGHTHAVFETASRQSARNLANKNIANPTAMLLASCIMLDYLQLHSYA 347
Query: 1013 AKIETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
I A A++++ T D+GG + DF I
Sbjct: 348 TSIRAAVLASMENKNVHTPDIGGQSTTLDFIHNI 381
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 65,959,718
Number of extensions: 1727517
Number of successful extensions: 5790
Number of sequences better than 1.0e-05: 18
Number of HSP's gapped: 5771
Number of HSP's successfully gapped: 19
Length of query: 570
Length of database: 18,874,504
Length adjustment: 108
Effective length of query: 462
Effective length of database: 15,274,216
Effective search space: 7056687792
Effective search space used: 7056687792
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002782
(1711 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_005521.1| isocitrate dehydrogenase [NAD] subunit alpha, m... 719 0.0
Alignment gi|NP_777280.1| isocitrate dehydrogenase [NAD] subunit beta, mi... 285 9e-77
Alignment gi|NP_008830.2| isocitrate dehydrogenase [NAD] subunit beta, mi... 278 1e-74
Alignment gi|NP_004126.1| isocitrate dehydrogenase [NAD] subunit gamma, m... 274 2e-73
Alignment gi|NP_777358.1| isocitrate dehydrogenase [NAD] subunit gamma, m... 261 2e-69
Alignment gi|NP_777281.1| isocitrate dehydrogenase [NAD] subunit beta, mi... 189 9e-48
>ref|NP_005521.1| isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor
[Homo sapiens].
Length = 366
Score = 719 bits (1856), Expect = 0.0
Identities = 356/363 (98%), Positives = 359/363 (98%)
Frame = +2
Query: 50 PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 229
PAWISKVSRLLGAFHN KQVTRGF GGV+TVTLIPGDGIGPEISAAVMKIFDAAKAPIQW
Sbjct: 4 PAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 63
Query: 230 EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 409
EERNVTAIQGPGGKWMIP EAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN
Sbjct: 64 EERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 123
Query: 410 VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA 589
VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA
Sbjct: 124 VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA 183
Query: 590 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP 769
FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAE+CKDIKFNEMYLDTVCLNMVQDP
Sbjct: 184 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDP 243
Query: 770 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 949
SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN
Sbjct: 244 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 303
Query: 950 PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
PTALLLSAVMMLRHMGLFDHAA+IE ACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK
Sbjct: 304 PTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 363
Query: 1130 DLD 1138
DLD
Sbjct: 364 DLD 366
>ref|NP_777280.1| isocitrate dehydrogenase [NAD] subunit beta, mitochondrial isoform b
precursor [Homo sapiens].
Length = 383
Score = 285 bits (729), Expect = 9e-77
Identities = 150/328 (45%), Positives = 213/328 (64%), Gaps = 3/328 (0%)
Frame = +2
Query: 140 VTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKM 319
VT++PGDG+GPE+ AV ++F AA P++++E +++ +Q + + + SM +NK+
Sbjct: 51 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLE-QVLSSMKENKV 109
Query: 320 GLKGPLKTPIA--AGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEG 493
+ G + TP+ S ++ LR+ DL+ANV S+ GY T + ++++V IRE TEG
Sbjct: 110 AIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEG 169
Query: 494 EYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLF 673
EYS +EH GV++ +K++T S+RIA+FAF+YA R VTAVHKANIM++ DGLF
Sbjct: 170 EYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLF 229
Query: 674 LQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLG 853
LQ C EVAE IKF M +D C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+GG G
Sbjct: 230 LQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAG 289
Query: 854 VTPSGNIGANGVAIFES-VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETA 1030
V P + A A+FE+ G+++ANPTA+LLSA MLRH+ L H++ I A
Sbjct: 290 VVPGESYSAE-YAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADA 348
Query: 1031 CFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
IK GK T D+GG A C DFTE +
Sbjct: 349 VKKVIKVGKVRTSDMGGYATCHDFTEAV 376
>ref|NP_008830.2| isocitrate dehydrogenase [NAD] subunit beta, mitochondrial isoform a
precursor [Homo sapiens].
Length = 385
Score = 278 bits (711), Expect = 1e-74
Identities = 146/328 (44%), Positives = 213/328 (64%), Gaps = 3/328 (0%)
Frame = +2
Query: 140 VTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKM 319
VT++PGDG+GPE+ AV ++F AA P++++E +++ +Q + + + SM +NK+
Sbjct: 51 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLE-QVLSSMKENKV 109
Query: 320 GLKGPLKTPIA--AGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEG 493
+ G + TP+ S ++ LR+ DL+ANV S+ GY T + ++++V IRE TEG
Sbjct: 110 AIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEG 169
Query: 494 EYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLF 673
EYS +EH GV++ +K++T S+RIA+FAF+YA R VTAVHKANIM++ DGLF
Sbjct: 170 EYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLF 229
Query: 674 LQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLG 853
LQ C EVAE IKF M +D C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+GG G
Sbjct: 230 LQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAG 289
Query: 854 VTPSGNIGANGVAIFES-VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETA 1030
V P + A A+FE+ G+++ANPTA+LLSA MLRH+ L H++ I A
Sbjct: 290 VVPGESYSAE-YAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADA 348
Query: 1031 CFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
IK GK T+D+GG + +DF + +
Sbjct: 349 VKKVIKVGKVRTRDMGGYSTTTDFIKSV 376
>ref|NP_004126.1| isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform a
precursor [Homo sapiens].
Length = 393
Score = 274 bits (701), Expect = 2e-73
Identities = 149/338 (44%), Positives = 214/338 (63%), Gaps = 3/338 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 52 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAI 107
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + D++I+ +R
Sbjct: 108 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVR 167
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ + R VTAVHKANIM++
Sbjct: 168 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKL 227
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ CREVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 228 GDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 287
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 288 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 346
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
I A A++ + T D+GG S+ +++ R ++
Sbjct: 347 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIR 384
>ref|NP_777358.1| isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform b
precursor [Homo sapiens].
Length = 380
Score = 261 bits (666), Expect = 2e-69
Identities = 143/310 (46%), Positives = 200/310 (64%), Gaps = 3/310 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 52 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAI 107
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + D++I+ +R
Sbjct: 108 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVR 167
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ + R VTAVHKANIM++
Sbjct: 168 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKL 227
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ CREVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 228 GDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 287
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 288 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 346
Query: 1016 KIETACFATI 1045
I A A++
Sbjct: 347 SIRKAVLASM 356
>ref|NP_777281.1| isocitrate dehydrogenase [NAD] subunit beta, mitochondrial isoform c
[Homo sapiens].
Length = 233
Score = 189 bits (479), Expect = 9e-48
Identities = 98/197 (49%), Positives = 132/197 (67%), Gaps = 1/197 (0%)
Frame = +2
Query: 527 GVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENC 706
GV++ +K++T S+RIA+FAF+YA R VTAVHKANIM++ DGLFLQ C EVAE
Sbjct: 29 GVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELY 88
Query: 707 KDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANG 886
IKF M +D C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+GG GV P + A
Sbjct: 89 PKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAE- 147
Query: 887 VAIFES-VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSL 1063
A+FE+ G+++ANPTA+LLSA MLRH+ L H++ I A IK GK
Sbjct: 148 YAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVR 207
Query: 1064 TKDLGGNAKCSDFTEEI 1114
T+D+GG + +DF + +
Sbjct: 208 TRDMGGYSTTTDFIKSV 224
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 64,946,493
Number of extensions: 1706389
Number of successful extensions: 5543
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 5536
Number of HSP's successfully gapped: 6
Length of query: 570
Length of database: 18,297,164
Length adjustment: 108
Effective length of query: 462
Effective length of database: 14,737,052
Effective search space: 6808518024
Effective search space used: 6808518024
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002782
(1711 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_083849.1| isocitrate dehydrogenase [NAD] subunit alpha, m... 719 0.0
Alignment gi|NP_570954.1| isocitrate dehydrogenase 3, beta subunit [Mus m... 283 4e-76
Alignment gi|NP_032349.1| isocitrate dehydrogenase [NAD] subunit gamma 1,... 275 1e-73
Alignment gi|NP_766489.1| probable isocitrate dehydrogenase [NAD] gamma 2... 247 2e-65
>ref|NP_083849.1| isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor
[Mus musculus].
Length = 366
Score = 719 bits (1857), Expect = 0.0
Identities = 356/362 (98%), Positives = 359/362 (99%)
Frame = +2
Query: 53 AWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWE 232
AW+SKVSRLLGAFHN KQVTRGFAGGV+TVTLIPGDGIGPEISA+VMKIFDAAKAPIQWE
Sbjct: 5 AWVSKVSRLLGAFHNTKQVTRGFAGGVQTVTLIPGDGIGPEISASVMKIFDAAKAPIQWE 64
Query: 233 ERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANV 412
ERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANV
Sbjct: 65 ERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANV 124
Query: 413 RPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAF 592
RPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITE ASKRIAEFAF
Sbjct: 125 RPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASKRIAEFAF 184
Query: 593 EYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPS 772
EYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPS
Sbjct: 185 EYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPS 244
Query: 773 QFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANP 952
QFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANP
Sbjct: 245 QFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANP 304
Query: 953 TALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKD 1132
TALLLSAVMMLRHMGLFDHAAKIE ACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKD
Sbjct: 305 TALLLSAVMMLRHMGLFDHAAKIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKD 364
Query: 1133 LD 1138
LD
Sbjct: 365 LD 366
>ref|NP_570954.1| isocitrate dehydrogenase 3, beta subunit [Mus musculus].
Length = 384
Score = 283 bits (723), Expect = 4e-76
Identities = 149/332 (44%), Positives = 216/332 (65%), Gaps = 3/332 (0%)
Frame = +2
Query: 128 GVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMD 307
G VT++PGDG+GPE+ AV ++F AA P++++E +++ +Q + + + SM
Sbjct: 46 GAFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFKEHHLSEVQNMASEEKLE-QVLSSMK 104
Query: 308 KNKMGLKGPLKTPIA--AGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRE 481
+NK+ + G + TP+ S ++ LR+ DL+ANV S+ GYKT + ++++V IRE
Sbjct: 105 ENKVAIIGKIYTPMEYKGELASYDMQLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIRE 164
Query: 482 NTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMS 661
TEGEYS +EH GV++ +K++T S+RIA+FAF+YA RS VTAVHKANIM++
Sbjct: 165 QTEGEYSSLEHESAKGVIECLKIVTRTKSQRIAKFAFDYATKKGRSKVTAVHKANIMKLG 224
Query: 662 DGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLI 841
DGLFLQ C EVAE IKF M +D C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+
Sbjct: 225 DGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLV 284
Query: 842 GGLGVTPSGNIGANGVAIFES-VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAK 1018
GG GV P + A A+FE+ G+++ANPTA+LLSA MLRH+ L H++
Sbjct: 285 GGAGVVPGESYSAE-YAVFETGARHPFAQAVGRNIANPTAMLLSATNMLRHLNLEYHSSM 343
Query: 1019 IETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
I A IK GK T+D+GG + +DF + +
Sbjct: 344 IADAVKKVIKAGKVRTRDMGGYSTTTDFIKSV 375
>ref|NP_032349.1| isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial
precursor [Mus musculus].
Length = 393
Score = 275 bits (702), Expect = 1e-73
Identities = 150/338 (44%), Positives = 215/338 (63%), Gaps = 3/338 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 52 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAI 107
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + D++I+ +R
Sbjct: 108 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVR 167
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ + R VTAVHKANIM++
Sbjct: 168 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKL 227
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ CREVA + I F+ M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 228 GDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 287
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 288 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 346
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
I A A++ + T D+GG S ++I R ++
Sbjct: 347 SIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIR 384
>ref|NP_766489.1| probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrial [Mus
musculus].
Length = 396
Score = 247 bits (630), Expect = 2e-65
Identities = 136/339 (40%), Positives = 204/339 (60%), Gaps = 3/339 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TV +IPGDGIGPE+ V KIF + P+ +EE VT+ A ++
Sbjct: 49 GGRHTVAMIPGDGIGPELMVHVKKIFRSNCVPVDFEEVWVTSTSNEEEI----NNALMAI 104
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG + T + A + S N R DLYA+V + G T + D++I+ +R
Sbjct: 105 RRNRVALKGNIATNHNLPARYKSHNTKFRTILDLYASVVHFKTFPGVMTRHKDIDILVVR 164
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEY+ +EH V GVV+S+K++T+ S RIA++AF+ A+ R VT VHKANIM++
Sbjct: 165 ENTEGEYTNLEHESVKGVVESLKIVTKTKSVRIADYAFKLAQKMGRKKVTVVHKANIMKL 224
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ C++VA + I M +D + +V P QFDV+VMPNLYG+I++ +C GL
Sbjct: 225 GDGLFLQCCKDVAAHYPQITLESMIIDNTTMQLVSKPQQFDVMVMPNLYGNIINSICTGL 284
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ P N G + AIFE D+A +++ANP A+LL++ +ML ++ L +A
Sbjct: 285 VGGSGIVPGANYG-DSYAIFEMGSKEIGKDLAHRNIANPVAMLLTSCIMLDYLDLQPYAT 343
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKD 1132
I +A A++++ T D+GG + E I +K+
Sbjct: 344 HIRSAVMASLQNKAVCTPDIGGQGNTASTVEYILHHMKE 382
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 55,443,639
Number of extensions: 1454581
Number of successful extensions: 4678
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 4666
Number of HSP's successfully gapped: 4
Length of query: 570
Length of database: 15,617,559
Length adjustment: 107
Effective length of query: 463
Effective length of database: 12,403,707
Effective search space: 5742916341
Effective search space used: 5742916341
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002782
(1711 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_001927373.3| PREDICTED: isocitrate dehydrogenase [NAD] su... 733 0.0
Alignment gi|NP_001038040.1| isocitrate dehydrogenase [NAD] subunit beta,... 280 1e-75
Alignment gi|XP_003135546.1| PREDICTED: isocitrate dehydrogenase [NAD] su... 273 2e-73
Alignment gi|XP_003135545.1| PREDICTED: isocitrate dehydrogenase [NAD] su... 273 2e-73
Alignment gi|XP_003360543.1| PREDICTED: isocitrate dehydrogenase [NAD] su... 273 2e-73
Alignment gi|XP_003135520.1| PREDICTED: isocitrate dehydrogenase [NAD] su... 273 2e-73
Alignment gi|XP_003361645.1| PREDICTED: isocitrate dehydrogenase [NAD] su... 256 2e-68
>ref|XP_001927373.3| PREDICTED: isocitrate dehydrogenase [NAD] subunit alpha,
mitochondrial [Sus scrofa].
Length = 366
Score = 733 bits (1891), Expect = 0.0
Identities = 363/363 (100%), Positives = 363/363 (100%)
Frame = +2
Query: 50 PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 229
PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW
Sbjct: 4 PAWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQW 63
Query: 230 EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 409
EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN
Sbjct: 64 EERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYAN 123
Query: 410 VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA 589
VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA
Sbjct: 124 VRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFA 183
Query: 590 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP 769
FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP
Sbjct: 184 FEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDP 243
Query: 770 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 949
SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN
Sbjct: 244 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMAN 303
Query: 950 PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK
Sbjct: 304 PTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 363
Query: 1130 DLD 1138
DLD
Sbjct: 364 DLD 366
>ref|NP_001038040.1| isocitrate dehydrogenase [NAD] subunit beta, mitochondrial [Sus
scrofa].
Length = 385
Score = 280 bits (717), Expect = 1e-75
Identities = 152/357 (42%), Positives = 222/357 (62%), Gaps = 3/357 (0%)
Frame = +2
Query: 53 AWISKVSRLLGAFHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWE 232
AW S + ++ ++ Q G VT++PGDG+GPE+ AV ++F AA P++++
Sbjct: 22 AWRSLSTSVVAQAASRSQAEDVRVEGAFPVTMLPGDGVGPELMHAVKEVFKAASVPVEFQ 81
Query: 233 ERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIA--AGHPSMNLLLRKTFDLYA 406
E +++ +Q + + + SM +NK+ + G + TP+ S ++ LR+ DL+A
Sbjct: 82 EHHLSEVQNMASEEKLE-QVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFA 140
Query: 407 NVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEF 586
NV S+ GY T + ++++V IRE TEGEYS +EH GV++ +K++T S+RIA+F
Sbjct: 141 NVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRTKSQRIAKF 200
Query: 587 AFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQD 766
AF+YA RS VTAVHKANIM++ DGLFLQ C EVAE IKF M +D C+ +VQ+
Sbjct: 201 AFDYATKKGRSKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQN 260
Query: 767 PSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDM 943
P QFDVLVMPNLYG+I+ +L AGL+GG GV P + A A+FE G+++
Sbjct: 261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAE-YAVFEMGARHPFAQAVGRNI 319
Query: 944 ANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDFTEEI 1114
ANPTA+LLSA MLRH+ L H+ I A IK GK T+D+GG + +DF + +
Sbjct: 320 ANPTAMLLSASNMLRHLNLEYHSNMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSV 376
>ref|XP_003135546.1| PREDICTED: isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial-like isoform 2 [Sus scrofa].
Length = 388
Score = 273 bits (699), Expect = 2e-73
Identities = 157/369 (42%), Positives = 225/369 (60%), Gaps = 9/369 (2%)
Frame = +2
Query: 50 PAWISKVSRLLGAFHNQKQVTRGFA------GGVKTVTLIPGDGIGPEISAAVMKIFDAA 211
PA + + +LGA + R F+ GG TVT+IPGDGIGPE+ V +F A
Sbjct: 19 PALLCRPWEVLGAHEAPR---RSFSPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHA 75
Query: 212 KAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTP--IAAGHPSMNLLLR 385
P+ +EE +V++ A ++ +N++ LKG ++T + H S N +LR
Sbjct: 76 CVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILR 131
Query: 386 KTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGA 565
+ DLYANV C S+ G T + DV+I+ +RENTEGEYS +EH V GVV+S+K+IT+
Sbjct: 132 TSLDLYANVIHCKSLPGVVTRHRDVDILIVRENTEGEYSSLEHESVAGVVESLKIITKAK 191
Query: 566 SKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTV 745
S RIAE+AF+ A+ R VTAVHKANIM++ DGLFLQ C+EVA I F M +D
Sbjct: 192 SLRIAEYAFKLAQETGRKKVTAVHKANIMKLGDGLFLQCCKEVAAGYPHITFENMIVDNT 251
Query: 746 CLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFE-SVHGTAP 922
+ +V P QFDV+VMPNLYG+I++++CAGL+GG G+ N G + A+FE + T
Sbjct: 252 TMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFETATRNTGK 310
Query: 923 DIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSDF 1102
IA K++ANPTA LL++ MML H+ L +A I A A++ + T D+GG S+
Sbjct: 311 SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEA 370
Query: 1103 TEEICRRVK 1129
++I R ++
Sbjct: 371 IQDIIRHIR 379
>ref|XP_003135545.1| PREDICTED: isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial-like isoform 1 [Sus scrofa].
Length = 392
Score = 273 bits (699), Expect = 2e-73
Identities = 157/370 (42%), Positives = 223/370 (60%), Gaps = 10/370 (2%)
Frame = +2
Query: 50 PAWISKVSRLLGA-------FHNQKQVTRGFAGGVKTVTLIPGDGIGPEISAAVMKIFDA 208
PA + + +LGA F Q GG TVT+IPGDGIGPE+ V +F
Sbjct: 19 PALLCRPWEVLGAHEAPRRSFSQQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRH 78
Query: 209 AKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTP--IAAGHPSMNLLL 382
A P+ +EE +V++ A ++ +N++ LKG ++T + H S N +L
Sbjct: 79 ACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNIL 134
Query: 383 RKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEG 562
R + DLYANV C S+ G T + DV+I+ +RENTEGEYS +EH V GVV+S+K+IT+
Sbjct: 135 RTSLDLYANVIHCKSLPGVVTRHRDVDILIVRENTEGEYSSLEHESVAGVVESLKIITKA 194
Query: 563 ASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDT 742
S RIAE+AF+ A+ R VTAVHKANIM++ DGLFLQ C+EVA I F M +D
Sbjct: 195 KSLRIAEYAFKLAQETGRKKVTAVHKANIMKLGDGLFLQCCKEVAAGYPHITFENMIVDN 254
Query: 743 VCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFE-SVHGTA 919
+ +V P QFDV+VMPNLYG+I++++CAGL+GG G+ N G + A+FE + T
Sbjct: 255 TTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFETATRNTG 313
Query: 920 PDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCSD 1099
IA K++ANPTA LL++ MML H+ L +A I A A++ + T D+GG S+
Sbjct: 314 KSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSE 373
Query: 1100 FTEEICRRVK 1129
++I R ++
Sbjct: 374 AIQDIIRHIR 383
>ref|XP_003360543.1| PREDICTED: isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial-like [Sus scrofa].
Length = 398
Score = 273 bits (698), Expect = 2e-73
Identities = 150/338 (44%), Positives = 213/338 (63%), Gaps = 3/338 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 57 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAI 112
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + DV+I+ +R
Sbjct: 113 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDVDILIVR 172
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ R VTAVHKANIM++
Sbjct: 173 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQETGRKKVTAVHKANIMKL 232
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ C+EVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 233 GDGLFLQCCKEVAAGYPHITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 292
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 293 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 351
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
I A A++ + T D+GG S+ ++I R ++
Sbjct: 352 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHIR 389
>ref|XP_003135520.1| PREDICTED: isocitrate dehydrogenase [NAD] subunit gamma,
mitochondrial [Sus scrofa].
Length = 450
Score = 273 bits (698), Expect = 2e-73
Identities = 150/338 (44%), Positives = 213/338 (63%), Gaps = 3/338 (0%)
Frame = +2
Query: 125 GGVKTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPPEAKESM 304
GG TVT+IPGDGIGPE+ V +F A P+ +EE +V++ A ++
Sbjct: 109 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAI 164
Query: 305 DKNKMGLKGPLKTP--IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIR 478
+N++ LKG ++T + H S N +LR + DLYANV C S+ G T + DV+I+ +R
Sbjct: 165 RRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHRDVDILIVR 224
Query: 479 ENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRM 658
ENTEGEYS +EH V GVV+S+K+IT+ S RIAE+AF+ A+ R VTAVHKANIM++
Sbjct: 225 ENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQETGRKKVTAVHKANIMKL 284
Query: 659 SDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGL 838
DGLFLQ C+EVA I F M +D + +V P QFDV+VMPNLYG+I++++CAGL
Sbjct: 285 GDGLFLQCCKEVAAGYPHITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 344
Query: 839 IGGLGVTPSGNIGANGVAIFE-SVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAA 1015
+GG G+ N G + A+FE + T IA K++ANPTA LL++ MML H+ L +A
Sbjct: 345 VGGPGLVAGANYG-HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 403
Query: 1016 KIETACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVK 1129
I A A++ + T D+GG S+ ++I R ++
Sbjct: 404 SIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHIR 441
>ref|XP_003361645.1| PREDICTED: isocitrate dehydrogenase [NAD] subunit beta,
mitochondrial-like [Sus scrofa].
Length = 354
Score = 256 bits (655), Expect = 2e-68
Identities = 139/306 (45%), Positives = 194/306 (63%), Gaps = 3/306 (0%)
Frame = +2
Query: 206 AAKAPIQWEERNVTAIQGPGGKWMIPPEAKESMDKNKMGLKGPLKTPIA--AGHPSMNLL 379
AA P++++E +++ +Q + + + SM +NK+ + G + TP+ S ++
Sbjct: 44 AASVPVEFQEHHLSEVQNMASEEKLE-QVLSSMKENKVAIIGKIHTPMEYKGELASYDMR 102
Query: 380 LRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITE 559
LR+ DL+ANV S+ GY T + ++++V IRE TEGEYS +EH GV++ +K++T
Sbjct: 103 LRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTR 162
Query: 560 GASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAENCKDIKFNEMYLD 739
S+RIA+FAF+YA RS VTAVHKANIM++ DGLFLQ C EVAE IKF M +D
Sbjct: 163 TKSQRIAKFAFDYATKKGRSKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIID 222
Query: 740 TVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFE-SVHGT 916
C+ +VQ+P QFDVLVMPNLYG+I+ +L AGL+GG GV P + A A+FE
Sbjct: 223 NCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAE-YAVFEMGARHP 281
Query: 917 APDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIETACFATIKDGKSLTKDLGGNAKCS 1096
G+++ANPTA+LLSA MLRH+ L H+ I A IK GK T D+GG A C
Sbjct: 282 FAQAVGRNIANPTAMLLSASNMLRHLNLEYHSNMIADAVKKVIKVGKVRTSDMGGYATCQ 341
Query: 1097 DFTEEI 1114
DFTE +
Sbjct: 342 DFTEAV 347
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 40,634,750
Number of extensions: 1071634
Number of successful extensions: 3609
Number of sequences better than 1.0e-05: 7
Number of HSP's gapped: 3603
Number of HSP's successfully gapped: 7
Length of query: 570
Length of database: 11,343,932
Length adjustment: 104
Effective length of query: 466
Effective length of database: 8,754,644
Effective search space: 4079664104
Effective search space used: 4079664104
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-002782
(1711 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_Chr07 1098 0.0
>Sscrofa_Chr07
|| Length = 134764511
Score = 1098 bits (554), Expect = 0.0
Identities = 633/658 (96%), Gaps = 3/658 (0%)
Strand = Plus / Minus
Query: 1056 agagcttaacaaaagatttgggaggcaacgcaaaatgctcagacttcacagaagaaatct 1115
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53313825 agagcttaacaaaagatttgggaggcaacgcaaaatgctcagacttcacagaagaaatct 53313766
Query: 1116 gtcgccgagtaaaagatttagattaagacggctccaaacagtatttgcatcagttgcttc 1175
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53313765 gtcgccgagtaaaagatttagattaagacggctccaaacagtatttgcatcagttgcttc 53313706
Query: 1176 taacggacgccatctcagcctgtattagaatacctcccattctatatgcatttgttgctt 1235
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53313705 taacggacgccatctcagcctgtattagaatacctcccattctatatgcatttgttgctt 53313646
Query: 1236 gtttcttgacagagtccatttctagacctggccttttcctaacaaaaatctgtgcaaagg 1295
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53313645 gtttcttgacagagtccatttctagacctggccttttcctaacaaaaatctgtgcaaagg 53313586
Query: 1296 gtgcaggcgatgtccctaggcctgctttcaaaggactttttccaagtgctcgttttattt 1355
||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct: 53313585 atgcaggcgatgtccctaggcctgctttcaaaggactttttccaagtgcttgttttattt 53313526
Query: 1356 attacatgtctatctggtatacnnnnnnngtacacttcaagtggactgtatcatttgcca 1415
|||||||||||||||||||| | |||||||||||||||||||||||||||||||
Sbjct: 53313525 attacatgtctatctggtatcctttttttgtacacttcaagtggactgtatcatttgcca 53313466
Query: 1416 ttgttaaccatcttacatttcagttaaaagtttttcttccactggtaataccatgataca 1475
|||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Sbjct: 53313465 ttgttaaccatcttacatttcagttaaaagtttttcttccactgtaaataccatgataca 53313406
Query: 1476 gaattgataagagaaaaatctaactttttaaagaaaatctctgatttccttgttttgtg- 1534
||||| |||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct: 53313405 gaattaataagagaaaaatctaactttttaaagaaaatctctgatttccttggtttgtga 53313346
Query: 1535 aaaatataacagaaataaagtaggatattgaggcaatagcacaagatgatcttcttataa 1594
||||||||||||||||||||||| |||||||||||||||||||||||||| |||||||||
Sbjct: 53313345 aaaatataacagaaataaagtagaatattgaggcaatagcacaagatgatattcttataa 53313286
Query: 1595 aactaancagggcacagaagaattttctggggaaag-ttccatcaaaacagtaaatacgg 1653
|||||| || |||||| |||| |||| ||||||| |||||||||||||||||||||||
Sbjct: 53313285 aactaaaca-ggcacaagagaaatttcctgggaaagtttccatcaaaacagtaaatacgg 53313227
Query: 1654 tatatttcttagtgacatggaaagtaaagactgatttgtccttttacgtgaattactg 1711
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53313226 tatatttcttagtgacatggaaagtaaagactgatttgtccttttacgtgaattactg 53313169
Score = 377 bits (190), Expect = e-101
Identities = 190/190 (100%)
Strand = Plus / Minus
Query: 328 aggccccttaaaaactccaatagctgctggtcacccatctatgaatttattgctgcgtaa 387
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53320525 aggccccttaaaaactccaatagctgctggtcacccatctatgaatttattgctgcgtaa 53320466
Query: 388 aacatttgacctttatgccaatgtccgaccgtgtgtctcaatcgaaggctataaaacccc 447
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53320465 aacatttgacctttatgccaatgtccgaccgtgtgtctcaatcgaaggctataaaacccc 53320406
Query: 448 ttacacagatgtaaatattgtcaccattcgcgagaacacggaaggagaatacagtggaat 507
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53320405 ttacacagatgtaaatattgtcaccattcgcgagaacacggaaggagaatacagtggaat 53320346
Query: 508 cgagcatgtg 517
||||||||||
Sbjct: 53320345 cgagcatgtg 53320336
Score = 305 bits (154), Expect = 4e-80
Identities = 154/154 (100%)
Strand = Plus / Minus
Query: 904 ggttcacgggactgcgcctgacatcgccggcaaggacatggccaaccccacagccctcct 963
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53316562 ggttcacgggactgcgcctgacatcgccggcaaggacatggccaaccccacagccctcct 53316503
Query: 964 gctcagtgctgtgatgatgctgcgccacatggggctcttcgaccacgctgcaaagattga 1023
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53316502 gctcagtgctgtgatgatgctgcgccacatggggctcttcgaccacgctgcaaagattga 53316443
Query: 1024 gaccgcgtgctttgctacgattaaggatgggaag 1057
||||||||||||||||||||||||||||||||||
Sbjct: 53316442 gaccgcgtgctttgctacgattaaggatgggaag 53316409
Score = 270 bits (136), Expect = 2e-69
Identities = 136/136 (100%)
Strand = Plus / Minus
Query: 517 gatcgtggatggagtcgtgcagagtatcaagctcatcaccgagggggcgagcaagcgcat 576
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53319862 gatcgtggatggagtcgtgcagagtatcaagctcatcaccgagggggcgagcaagcgcat 53319803
Query: 577 cgccgagttcgcctttgaatacgcacggaacaatcaccggagcaacgtcacggcggtgca 636
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53319802 cgccgagttcgcctttgaatacgcacggaacaatcaccggagcaacgtcacggcggtgca 53319743
Query: 637 caaagccaacatcatg 652
||||||||||||||||
Sbjct: 53319742 caaagccaacatcatg 53319727
Score = 230 bits (116), Expect = 2e-57
Identities = 116/116 (100%)
Strand = Plus / Minus
Query: 215 gcgcctattcagtgggaggaacggaatgtcactgccattcaaggaccgggaggaaagtgg 274
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53322507 gcgcctattcagtgggaggaacggaatgtcactgccattcaaggaccgggaggaaagtgg 53322448
Query: 275 atgatccctccggaggccaaggagtccatggataagaacaagatgggcttgaaagg 330
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53322447 atgatccctccggaggccaaggagtccatggataagaacaagatgggcttgaaagg 53322392
Score = 204 bits (103), Expect = 1e-49
Identities = 103/103 (100%)
Strand = Plus / Minus
Query: 652 gcgcatgtcagatgggctttttctgcaaaaatgcagggaagttgcagaaaactgtaaaga 711
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53318622 gcgcatgtcagatgggctttttctgcaaaaatgcagggaagttgcagaaaactgtaaaga 53318563
Query: 712 tattaaatttaatgagatgtaccttgatacagtatgtttgaat 754
|||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53318562 tattaaatttaatgagatgtaccttgatacagtatgtttgaat 53318520
Score = 176 bits (89), Expect = 3e-41
Identities = 89/89 (100%)
Strand = Plus / Minus
Query: 818 agtgacctgtgtgcgggattgatcggaggtcttggtgtgacaccaagcggcaacattgga 877
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53317343 agtgacctgtgtgcgggattgatcggaggtcttggtgtgacaccaagcggcaacattgga 53317284
Query: 878 gccaacggggttgcaatctttgagtcggt 906
|||||||||||||||||||||||||||||
Sbjct: 53317283 gccaacggggttgcaatctttgagtcggt 53317255
Score = 168 bits (85), Expect = 6e-39
Identities = 85/85 (100%)
Strand = Plus / Minus
Query: 131 gttaagacagtaactttaattccaggcgatggaattggcccagaaatttcagcggcagtt 190
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53324125 gttaagacagtaactttaattccaggcgatggaattggcccagaaatttcagcggcagtt 53324066
Query: 191 atgaagatttttgatgctgccaaag 215
|||||||||||||||||||||||||
Sbjct: 53324065 atgaagatttttgatgctgccaaag 53324041
Score = 135 bits (68), Expect = 9e-29
Identities = 68/68 (100%)
Strand = Plus / Minus
Query: 2 actattgccgccgccgtcgccgcggtcagagggagcgcgatggctgggccagcgtggatc 61
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53330692 actattgccgccgccgtcgccgcggtcagagggagcgcgatggctgggccagcgtggatc 53330633
Query: 62 tccaaggt 69
||||||||
Sbjct: 53330632 tccaaggt 53330625
Score = 133 bits (67), Expect = 4e-28
Identities = 67/67 (100%)
Strand = Plus / Minus
Query: 66 aggtctctcggctgctgggggccttccacaaccaaaaacaggtgaccagaggttttgctg 125
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53325744 aggtctctcggctgctgggggccttccacaaccaaaaacaggtgaccagaggttttgctg 53325685
Query: 126 gtggtgt 132
|||||||
Sbjct: 53325684 gtggtgt 53325678
Score = 129 bits (65), Expect = 5e-27
Identities = 65/65 (100%)
Strand = Plus / Minus
Query: 755 atggtccaagatccgtctcagtttgatgttcttgttatgccaaatttgtatggagacatt 814
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53318418 atggtccaagatccgtctcagtttgatgttcttgttatgccaaatttgtatggagacatt 53318359
Query: 815 cttag 819
|||||
Sbjct: 53318358 cttag 53318354
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 52,892,817
Number of extensions: 369
Number of successful extensions: 369
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 367
Number of HSP's successfully gapped: 12
Length of query: 1711
Length of database: 2,808,509,378
Length adjustment: 22
Effective length of query: 1689
Effective length of database: 2,808,408,574
Effective search space: 4743402081486
Effective search space used: 4743402081486
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)