Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008727
(1773 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001076946.1| C-1-tetrahydrofolate synthase, cytoplasmic [... 857 0.0
Alignment gi|XP_002690382.1| PREDICTED: Monofunctional C1-tetrahydrofolat... 384 e-106
Alignment gi|NP_001069486.1| monofunctional C1-tetrahydrofolate synthase,... 382 e-106
Alignment gi|NP_001069223.1| bifunctional methylenetetrahydrofolate dehyd... 194 3e-49
Alignment gi|NP_001193024.1| probable bifunctional methylenetetrahydrofol... 181 2e-45
Alignment gi|XP_002688398.1| PREDICTED: methylenetetrahydrofolate dehydro... 181 2e-45
>ref|NP_001076946.1| C-1-tetrahydrofolate synthase, cytoplasmic [Bos taurus].
Length = 935
Score = 857 bits (2214), Expect = 0.0
Identities = 426/493 (86%), Positives = 454/493 (92%)
Frame = +3
Query: 252 MAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAA 431
MAPAEILNGKVVSAQI+E+LK++V QMKE+VPGF PGLAILQVGNRDDSNLYIN+KLKAA
Sbjct: 1 MAPAEILNGKVVSAQIKERLKNQVIQMKEEVPGFIPGLAILQVGNRDDSNLYINMKLKAA 60
Query: 432 KEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAP 611
+EIGIKA HIKLPRTATESEVLKCITSLNEDLTVHGFI+QLPLDSENPIN E L+NAIAP
Sbjct: 61 EEIGIKATHIKLPRTATESEVLKCITSLNEDLTVHGFIVQLPLDSENPINTETLVNAIAP 120
Query: 612 EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAP 791
EKDVDGL SI+AGKLARGDL++CFIPCTPKGCLELIK+TGVQIAGR AVV+GRSKIVGAP
Sbjct: 121 EKDVDGLNSISAGKLARGDLSNCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAP 180
Query: 792 MHDLLLWNHATVTTCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINC 971
MHDLLLWNHATVTTCHSKTANL EE++KGDI+VVAAG+PEMVKGEWIKPGA+VIDCGIN
Sbjct: 181 MHDLLLWNHATVTTCHSKTANLKEEISKGDILVVAAGQPEMVKGEWIKPGAIVIDCGINY 240
Query: 972 VPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRP 1151
V DDTKP+GK+IVGDVAY+EAKERA FITPVPGGVGPMTVAMLMQSTVESA+ FLE F+P
Sbjct: 241 VTDDTKPNGKKIVGDVAYTEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLENFKP 300
Query: 1152 GKWIIQYNKLNLKTPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSL 1331
GKWIIQYNKLNLKTPVPSDIDISRS KPKPIGNLARE+GL +EEVELYGETKAKVLLS+L
Sbjct: 301 GKWIIQYNKLNLKTPVPSDIDISRSYKPKPIGNLAREVGLRTEEVELYGETKAKVLLSAL 360
Query: 1332 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI 1511
ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALG HLHQNVFACVRQPSQGPTFGI
Sbjct: 361 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGTHLHQNVFACVRQPSQGPTFGI 420
Query: 1512 KXXXXXXXYSQVIPMEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLV 1691
K YSQVIPMEEFNLHLTGDIH RMFHE+TQTDKALF RLV
Sbjct: 421 KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHEETQTDKALFNRLV 480
Query: 1692 PSVNGVRKFSDIQ 1730
PSVNGVRKFSDIQ
Sbjct: 481 PSVNGVRKFSDIQ 493
>ref|XP_002690382.1| PREDICTED: Monofunctional C1-tetrahydrofolate synthase,
mitochondrial-like [Bos taurus].
Length = 990
Score = 384 bits (985), Expect = e-106
Identities = 213/479 (44%), Positives = 291/479 (60%)
Frame = +3
Query: 294 QIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPR 473
++ + K ++ ++E+ P F P LAI+Q G D NL V A+E G+ HI LP
Sbjct: 75 EVIQNSKEVLSLLQEKTPTFKPVLAIIQAG---DDNLMQEVNQNLAEEAGLNITHICLPA 131
Query: 474 TATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGK 653
+ E E++ I +NED VHG LQ+ S + ++NA+ PEKDVDGLT +N GK
Sbjct: 132 ESGEDEIIDEILKINEDSRVHGLALQIAETSFS----NKILNALKPEKDVDGLTDVNLGK 187
Query: 654 LARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTT 833
L RGD ++CFI + +EL++++GV + G++ +VIG + A + L + +
Sbjct: 188 LVRGDAHECFISPVARAVIELLEKSGVSLDGKKILVIGAHGSLEATLQCLFQRKGSMTMS 247
Query: 834 CHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVG 1013
KT L ++ + DIVV+ + KPE + WI+PG V +C + + + G
Sbjct: 248 SQWKTPQLQGKLQEADIVVLGSPKPEEIPLSWIQPGTTVFNCSHDFLSGKAACISSGVHG 307
Query: 1014 DVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLKT 1193
I+P+ V + A+ +Q+ V S +L + + +W + KL +
Sbjct: 308 -------------ISPIAEDVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLS 354
Query: 1194 PVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVVV 1373
PVPSDI+ISR+ PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+V
Sbjct: 355 PVPSDIEISRAQTPKAVEILAKEIGLLADEIEIYGKSKAKVRLSLLERLKDQADGKYVLV 414
Query: 1374 TGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIP 1553
GITPTPLGEGKST TIGLVQAL AHL+ N FAC+RQPSQGPTFG+K Y+QVIP
Sbjct: 415 AGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIP 474
Query: 1554 MEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
MEEFNLHLTGDIH R+ HE TQTDKAL+ RLVPSVNGVR+FS IQ
Sbjct: 475 MEEFNLHLTGDIHAITAANNLLAAAIDARILHENTQTDKALYNRLVPSVNGVREFSKIQ 533
>ref|NP_001069486.1| monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
[Bos taurus].
Length = 975
Score = 382 bits (982), Expect = e-106
Identities = 213/479 (44%), Positives = 290/479 (60%)
Frame = +3
Query: 294 QIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPR 473
++ + K ++ ++E+ P F P LAI+Q G D NL V A+E G+ HI LP
Sbjct: 75 EVIQNSKEVLSLLQEKTPTFKPVLAIIQAG---DDNLMQEVNQNLAEEAGLNITHICLPA 131
Query: 474 TATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGK 653
+ E E++ I +NED VHG LQ+ S + ++NA+ PEKDVDGLT +N GK
Sbjct: 132 ESGEDEIIDEILKINEDSRVHGLALQIAETSFS----NKILNALKPEKDVDGLTDVNLGK 187
Query: 654 LARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTT 833
L RGD + CFI + +EL++++GV + G++ +VIG + A + L + +
Sbjct: 188 LVRGDAHGCFISPVARAVIELLEKSGVSLDGKKILVIGAHGSLEATLQCLFQRKGSMTMS 247
Query: 834 CHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVG 1013
KT L ++ + DIVV+ + KPE + WI+PG V +C + + + G
Sbjct: 248 SQWKTPQLQGKLQEADIVVLGSPKPEEIPLSWIQPGTTVFNCSHDFLSGKAACISSGVHG 307
Query: 1014 DVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLKT 1193
I+P+ V + A+ +Q+ V S +L + + +W + KL +
Sbjct: 308 -------------ISPIAEDVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLS 354
Query: 1194 PVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVVV 1373
PVPSDI+ISR+ PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+V
Sbjct: 355 PVPSDIEISRAQTPKAVEILAKEIGLLADEIEIYGKSKAKVRLSLLERLKDQADGKYVLV 414
Query: 1374 TGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIP 1553
GITPTPLGEGKST TIGLVQAL AHL+ N FAC+RQPSQGPTFG+K Y+QVIP
Sbjct: 415 AGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIP 474
Query: 1554 MEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
MEEFNLHLTGDIH R+ HE TQTDKAL+ RLVPSVNGVR+FS IQ
Sbjct: 475 MEEFNLHLTGDIHAITAANNLLAAAIDARILHENTQTDKALYNRLVPSVNGVREFSKIQ 533
>ref|NP_001069223.1| bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase,
mitochondrial [Bos taurus].
Length = 350
Score = 194 bits (492), Expect = 3e-49
Identities = 123/312 (39%), Positives = 180/312 (57%), Gaps = 13/312 (4%)
Frame = +3
Query: 240 LTKVMAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVK 419
L+ V A +++G+ ++ QI+++++ +V + P L+++ VG S Y+ K
Sbjct: 30 LSAVRNEAVVISGRKLAEQIKQEVRQEVEEWVAS-GNKRPHLSVVLVGENPASQSYVLNK 88
Query: 420 LKAAKEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALIN 599
+AA +GI + I P + +E E+L I LN D V G ++QLPL I+ + N
Sbjct: 89 TRAAASVGINSETILKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEH--IDERKVCN 146
Query: 600 AIAPEKDVDGLTSINAGKLARGDLNDC-FIPCTPKGCLELIKQTGVQIAGRQAVVIGRSK 776
A++P+KDVDG IN G++ L+ C +P TP G E+IK+TG+ G+ VV GRSK
Sbjct: 147 AVSPDKDVDGFHVINVGRMC---LDQCSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSK 203
Query: 777 IVGAPMHDLLLWN--------HATVTTCHSKTANLNEEVNK----GDIVVVAAGKPEMVK 920
VG P+ LL + ATVT H T EE+ K DIV+ AAG P ++
Sbjct: 204 NVGMPIAMLLHTDGAHERPGGDATVTISHRYTPK--EELKKHTALADIVISAAGIPNLIT 261
Query: 921 GEWIKPGAVVIDCGINCVPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAML 1100
+ IK GA VID GIN + D K +VGDV + K++AG+ITPVPGGVGPMTVAML
Sbjct: 262 ADMIKEGAAVIDVGINRIQDPITAKPK-LVGDVDFEGVKKKAGYITPVPGGVGPMTVAML 320
Query: 1101 MQSTVESAEHFL 1136
M++T+ +A+ L
Sbjct: 321 MKNTIIAAKKVL 332
>ref|NP_001193024.1| probable bifunctional methylenetetrahydrofolate
dehydrogenase/cyclohydrolase 2 [Bos taurus].
Length = 347
Score = 181 bits (458), Expect = 2e-45
Identities = 115/303 (37%), Positives = 176/303 (58%), Gaps = 11/303 (3%)
Frame = +3
Query: 261 AEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEI 440
A I++G ++ QI+++++ V + P L+I+ VG+ S+ Y+ K+KAA +
Sbjct: 51 AIIISGTEMAKQIQKEIQHGVDSWVS-LGNRRPHLSIILVGDNPASHTYVRRKIKAAAAV 109
Query: 441 GIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKD 620
GI + I P+ ++ E+L LN D V G ++QLPL ++ + N IAPEKD
Sbjct: 110 GICSEIILKPKDVSQEELLDITDQLNADPRVSGILVQLPLPDH--MDERTVCNGIAPEKD 167
Query: 621 VDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHD 800
VDG IN G+L + IP T E+I++TG++ G+ VV GRSK VG P+
Sbjct: 168 VDGFHIINIGRLCLDQHS--LIPATASAVWEIIRRTGIETFGKNVVVAGRSKNVGMPIAM 225
Query: 801 LLLWN--------HATVTTCHSKTANLNEEVNK--GDIVVVAAGKPEMVKGEWIKPGAVV 950
LL + ATVT H T +++ D+++VAAG P+++ + +K GA V
Sbjct: 226 LLHTDGEHERPGGDATVTIAHRYTPKEQLKIHTLLADVIIVAAGIPKLITSDMVKEGAAV 285
Query: 951 IDCGINCVPDDTKPSGK-RIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAE 1127
ID GIN V D +GK ++VGDV + K++A FITPVPGGVGPMTVAML+++T+ +A+
Sbjct: 286 IDVGINYVYDPI--TGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 343
Query: 1128 HFL 1136
+
Sbjct: 344 KII 346
>ref|XP_002688398.1| PREDICTED: methylenetetrahydrofolate dehydrogenase (NADP+ dependent)
2-like [Bos taurus].
Length = 347
Score = 181 bits (458), Expect = 2e-45
Identities = 115/303 (37%), Positives = 176/303 (58%), Gaps = 11/303 (3%)
Frame = +3
Query: 261 AEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEI 440
A I++G ++ QI+++++ V + P L+I+ VG+ S+ Y+ K+KAA +
Sbjct: 51 AIIISGTEMAKQIQKEIQHGVDSWVS-LGNRRPHLSIILVGDNPASHTYVRRKIKAAAAV 109
Query: 441 GIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKD 620
GI + I P+ ++ E+L LN D V G ++QLPL ++ + N IAPEKD
Sbjct: 110 GICSEIILKPKDVSQEELLDITDQLNADPRVSGILVQLPLPDH--MDERTVCNGIAPEKD 167
Query: 621 VDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHD 800
VDG IN G+L + IP T E+I++TG++ G+ VV GRSK VG P+
Sbjct: 168 VDGFHIINIGRLCLDQHS--LIPATASAVWEIIRRTGIETFGKNVVVAGRSKNVGMPIAM 225
Query: 801 LLLWN--------HATVTTCHSKTANLNEEVNK--GDIVVVAAGKPEMVKGEWIKPGAVV 950
LL + ATVT H T +++ D+++VAAG P+++ + +K GA V
Sbjct: 226 LLHTDGEHERPGGDATVTIAHRYTPKEQLKIHTLLADVIIVAAGIPKLITSDMVKEGAAV 285
Query: 951 IDCGINCVPDDTKPSGK-RIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAE 1127
ID GIN V D +GK ++VGDV + K++A FITPVPGGVGPMTVAML+++T+ +A+
Sbjct: 286 IDVGINYVYDPI--TGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 343
Query: 1128 HFL 1136
+
Sbjct: 344 KII 346
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 72,495,517
Number of extensions: 2080870
Number of successful extensions: 6834
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 6775
Number of HSP's successfully gapped: 6
Length of query: 591
Length of database: 17,681,374
Length adjustment: 108
Effective length of query: 483
Effective length of database: 14,107,870
Effective search space: 6814101210
Effective search space used: 6814101210
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008727
(1773 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_537476.2| PREDICTED: similar to C-1-tetrahydrofolate synt... 863 0.0
Alignment gi|XP_533450.2| PREDICTED: similar to methylenetetrahydrofolate... 378 e-105
Alignment gi|XP_540223.2| PREDICTED: similar to Bifunctional methylenetet... 186 5e-47
Alignment gi|XP_539316.2| PREDICTED: similar to methylenetetrahydrofolate... 183 4e-46
Alignment gi|XP_854263.1| PREDICTED: similar to methylenetetrahydrofolate... 156 5e-38
Alignment gi|XP_850609.1| PREDICTED: similar to Bifunctional methylenetet... 65 2e-10
>ref|XP_537476.2| PREDICTED: similar to C-1-tetrahydrofolate synthase, cytoplasmic
(C1-THF synthase) [Canis familiaris].
Length = 935
Score = 863 bits (2230), Expect = 0.0
Identities = 428/493 (86%), Positives = 457/493 (92%)
Frame = +3
Query: 252 MAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAA 431
MAPAEILNGKVVSAQIRE+LK++V QMKEQVPGF PGLAILQVG+RDDSNLYINVKLKAA
Sbjct: 1 MAPAEILNGKVVSAQIRERLKNEVTQMKEQVPGFIPGLAILQVGDRDDSNLYINVKLKAA 60
Query: 432 KEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAP 611
+EIGI+A HIKLP+TATESEVLK +TSLNEDLTVHGFI+QLPLDSENPIN EA+INAI P
Sbjct: 61 EEIGIRATHIKLPKTATESEVLKYVTSLNEDLTVHGFIVQLPLDSENPINTEAVINAIVP 120
Query: 612 EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAP 791
EKDVDGLTSI+AGKLARGDL DCFIPCTPKGCLELIK+TGVQIAGR AVV+GRSKIVGAP
Sbjct: 121 EKDVDGLTSISAGKLARGDLKDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAP 180
Query: 792 MHDLLLWNHATVTTCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINC 971
MHDLLLWNHATVTTCHSKTANL+EEVNKGDI+VVA G+PEMVKGEWIKPGA+VIDCGIN
Sbjct: 181 MHDLLLWNHATVTTCHSKTANLSEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINY 240
Query: 972 VPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRP 1151
+PDDTKP+G+++VGDVAY+EAKERAGFITPVPGGVGPMTVAMLMQSTVESA+ FLEKF+P
Sbjct: 241 IPDDTKPNGRKVVGDVAYNEAKERAGFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKP 300
Query: 1152 GKWIIQYNKLNLKTPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSL 1331
GKW+IQYN LNLKTPVPSDIDISRSCKPKPIG+LAREIGLLSEEVELYG+TKAKVLLS+L
Sbjct: 301 GKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGSLAREIGLLSEEVELYGDTKAKVLLSAL 360
Query: 1332 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI 1511
ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI
Sbjct: 361 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI 420
Query: 1512 KXXXXXXXYSQVIPMEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLV 1691
K YSQVIPMEEFNLHLTGDIH RMFHE TQTDKALF RLV
Sbjct: 421 KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHELTQTDKALFNRLV 480
Query: 1692 PSVNGVRKFSDIQ 1730
PSVNGVRKFSDIQ
Sbjct: 481 PSVNGVRKFSDIQ 493
>ref|XP_533450.2| PREDICTED: similar to methylenetetrahydrofolate dehydrogenase (NADP+
dependent) 1-like [Canis familiaris].
Length = 923
Score = 378 bits (971), Expect = e-105
Identities = 212/479 (44%), Positives = 297/479 (62%)
Frame = +3
Query: 294 QIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPR 473
++ + K ++ ++E+ P F P LAI+Q G D NL + A+E G+ HI LP
Sbjct: 22 EVIQNSKEVLSLLQEKNPTFKPVLAIIQAG---DDNLMQEINQNLAEEAGLNITHICLPP 78
Query: 474 TATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGK 653
++E E++ I +NED VHG LQ+ SE+ + + L NA+ PEKDVDG+T IN G+
Sbjct: 79 DSSEDEIIDEILKINEDTRVHGLALQI---SESSFSNKVL-NALKPEKDVDGVTDINLGR 134
Query: 654 LARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTT 833
L RGD ++C++ + +EL++++GV + G++ ++IG + A + L + T
Sbjct: 135 LVRGDAHECYVSPVARAVIELLEKSGVNLDGKKILIIGAHGSLEAALQCLFQRRGSMTMT 194
Query: 834 CHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVG 1013
KT +L ++ + DIVV+ + KPE + WI+PG V++C + + SGK G
Sbjct: 195 SQWKTPHLQSKLLESDIVVLGSPKPEEIPLSWIQPGTTVLNCSHDFL------SGKLGCG 248
Query: 1014 DVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLKT 1193
+ G I V + A+ +Q+ V S +L + + +W + KL +
Sbjct: 249 SLGV----HFNGLIAE--DDVSLLAAALRIQNMVSSGRRWLREQQHARWRLHCLKLQPLS 302
Query: 1194 PVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVVV 1373
PVPSD++ISR+ PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+V
Sbjct: 303 PVPSDLEISRAQTPKAVDVLAKEIGLLADEIEIYGKSKAKVRLSLLERLKDQADGKYVLV 362
Query: 1374 TGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIP 1553
GITPTPLGEGKST T+GLVQAL AHL+ N FAC+RQPSQGPTFG+K Y+QVIP
Sbjct: 363 AGITPTPLGEGKSTVTVGLVQALTAHLNINSFACLRQPSQGPTFGVKGGAAGGGYAQVIP 422
Query: 1554 MEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
MEEFNLHLTGDIH R+ HE TQTDKAL+ RLVPSVNGVR+FS IQ
Sbjct: 423 MEEFNLHLTGDIHAITAANNLLAAAIDTRILHENTQTDKALYNRLVPSVNGVREFSSIQ 481
>ref|XP_540223.2| PREDICTED: similar to Bifunctional methylenetetrahydrofolate
dehydrogenase/cyclohydrolase, mitochondrial precursor
[Canis familiaris].
Length = 398
Score = 186 bits (473), Expect = 5e-47
Identities = 117/302 (38%), Positives = 171/302 (56%), Gaps = 10/302 (3%)
Frame = +3
Query: 261 AEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEI 440
A I++G+ ++ QI+++++ +V + P L+++ VG S+ Y+ K +AA E+
Sbjct: 85 AVIISGRKLAQQIKQEVRQEVEEWVAS-GNKRPHLSVVLVGENPASHSYVLNKTRAAAEV 143
Query: 441 GIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKD 620
G+ +T E VL I LN D V G ++QLPL I+ + NA++P+KD
Sbjct: 144 GMCHSQTLTAKTQAEEAVLNLINKLNNDDNVDGLLVQLPLPEH--IDERKVCNAVSPDKD 201
Query: 621 VDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHD 800
VDG IN G++ + +P TP G E+IK+TG+ G+ VV GRSK VG P+
Sbjct: 202 VDGFHVINVGRMCLDQYS--MLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAM 259
Query: 801 LLLWN--------HATVTTCHSKTAN--LNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVV 950
LL + ATVT H T L + DIV+ AAG P ++ + IK GA V
Sbjct: 260 LLHTDGAHERPGGDATVTISHRYTPKEQLKKHTVLADIVISAAGIPNLITADMIKEGAAV 319
Query: 951 IDCGINCVPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEH 1130
ID GIN V D K +VGDV + +++AG+ITPVPGGVGPMTVAMLM++T+ +A+
Sbjct: 320 IDVGINRVQDPITAKPK-LVGDVDFEGVRKKAGYITPVPGGVGPMTVAMLMKNTIIAAKK 378
Query: 1131 FL 1136
L
Sbjct: 379 VL 380
>ref|XP_539316.2| PREDICTED: similar to methylenetetrahydrofolate dehydrogenase (NADP+
dependent) 2-like [Canis familiaris].
Length = 310
Score = 183 bits (465), Expect = 4e-46
Identities = 117/303 (38%), Positives = 174/303 (57%), Gaps = 11/303 (3%)
Frame = +3
Query: 261 AEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEI 440
A I++G ++ QI+++++ V + P L+I+ VG+ S+ Y+ K++AA +
Sbjct: 14 AIIISGTEMAKQIQKEIQRGVESWIS-LGNRRPHLSIILVGDNPASHTYVRKKIRAASAV 72
Query: 441 GIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKD 620
GI + I P+ ++ E+L LN D V G ++QLPL ++ + N IAPEKD
Sbjct: 73 GICSEIILKPKDVSQEELLDITDQLNMDPRVSGILVQLPLPDH--VDERTVCNGIAPEKD 130
Query: 621 VDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHD 800
VDG IN G+L + IP T E+IK+TG++ G+ VV GRSK VG P+
Sbjct: 131 VDGFHIINIGRLCLDQHS--LIPATASAVWEIIKRTGIETFGKNVVVAGRSKNVGMPIAM 188
Query: 801 LLLWN--------HATVTTCHSKTAN--LNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVV 950
LL + ATVT H T L DI++VAAG P+++ + +K GA V
Sbjct: 189 LLHTDGEHERPGGDATVTIAHRYTPKEQLKIHTQLADIIIVAAGIPKLITSDMVKEGAAV 248
Query: 951 IDCGINCVPDDTKPSGK-RIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAE 1127
ID GIN V D +GK ++VGDV + K++A FITPVPGGVGPMTVAML+++T+ +A+
Sbjct: 249 IDVGINYVHDPV--TGKTKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAAK 306
Query: 1128 HFL 1136
+
Sbjct: 307 KLI 309
>ref|XP_854263.1| PREDICTED: similar to methylenetetrahydrofolate dehydrogenase (NADP+
dependent), methenyltetrahydrofolate cyclohydrolase,
formyltetrahydrofolate synthase [Canis familiaris].
Length = 144
Score = 156 bits (395), Expect = 5e-38
Identities = 79/113 (69%), Positives = 84/113 (74%)
Frame = +3
Query: 1392 PLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIPMEEFNL 1571
PLGEGKST TIGLVQALG HLHQNVFACVRQPSQGPTFGIK YSQVIPME+F+L
Sbjct: 2 PLGEGKSTATIGLVQALGTHLHQNVFACVRQPSQGPTFGIKDGIARGGYSQVIPMEQFDL 61
Query: 1572 HLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
+LTGDIH +MFH+ QTDK LF LVPSVNGVRKFSDIQ
Sbjct: 62 YLTGDIHAIAAVNNLVAAAIDAQMFHKLMQTDKGLFNHLVPSVNGVRKFSDIQ 114
>ref|XP_850609.1| PREDICTED: similar to Bifunctional methylenetetrahydrofolate
dehydrogenase/cyclohydrolase, mitochondrial precursor
[Canis familiaris].
Length = 239
Score = 65.1 bits (157), Expect = 2e-10
Identities = 38/86 (44%), Positives = 55/86 (63%)
Frame = +3
Query: 879 DIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVGDVAYSEAKERAGFIT 1058
+IV+ A G ++ + IK GA +I+ GIN V D K +VGDV + ++ A +IT
Sbjct: 116 EIVISAVGILNLITADIIKEGAALINVGINRVQDPILSKAK-LVGDVDFEGIRKNANYIT 174
Query: 1059 PVPGGVGPMTVAMLMQSTVESAEHFL 1136
PV GVGPMTVAMLM++T+ +A+ L
Sbjct: 175 PV-WGVGPMTVAMLMKNTIIAAKKVL 199
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 75,433,557
Number of extensions: 2137241
Number of successful extensions: 6731
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 6698
Number of HSP's successfully gapped: 6
Length of query: 591
Length of database: 18,874,504
Length adjustment: 108
Effective length of query: 483
Effective length of database: 15,274,216
Effective search space: 7377446328
Effective search space used: 7377446328
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008727
(1773 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_001924364.2| PREDICTED: c-1-tetrahydrofolate synthase, cy... 929 0.0
Alignment gi|XP_003129156.2| PREDICTED: probable bifunctional methylenete... 108 7e-24
Alignment gi|XP_003361784.1| PREDICTED: bifunctional protein FolD-like, p... 50 3e-06
>ref|XP_001924364.2| PREDICTED: c-1-tetrahydrofolate synthase, cytoplasmic [Sus scrofa].
Length = 935
Score = 929 bits (2400), Expect = 0.0
Identities = 470/493 (95%), Positives = 470/493 (95%)
Frame = +3
Query: 252 MAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAA 431
MAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAA
Sbjct: 1 MAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAA 60
Query: 432 KEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAP 611
KEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAP
Sbjct: 61 KEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAP 120
Query: 612 EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAP 791
EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAP
Sbjct: 121 EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAP 180
Query: 792 MHDLLLWNHATVTTCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINC 971
MHDLLLWNHATVTTCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINC
Sbjct: 181 MHDLLLWNHATVTTCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINC 240
Query: 972 VPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRP 1151
VPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRP
Sbjct: 241 VPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRP 300
Query: 1152 GKWIIQYNKLNLKTPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSL 1331
GKWIIQYNKLNLKTPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSL
Sbjct: 301 GKWIIQYNKLNLKTPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSL 360
Query: 1332 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI 1511
ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI
Sbjct: 361 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI 420
Query: 1512 KXXXXXXXYSQVIPMEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLV 1691
K YSQVIPMEEFNLHLTGDIH RMFHEQTQTDKALF RLV
Sbjct: 421 KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMFHEQTQTDKALFNRLV 480
Query: 1692 PSVNGVRKFSDIQ 1730
PSVNGVRKFSDIQ
Sbjct: 481 PSVNGVRKFSDIQ 493
>ref|XP_003129156.2| PREDICTED: probable bifunctional methylenetetrahydrofolate
dehydrogenase/cyclohydrolase 2-like [Sus scrofa].
Length = 343
Score = 108 bits (271), Expect = 7e-24
Identities = 71/192 (36%), Positives = 106/192 (55%)
Frame = +3
Query: 231 GSGLTKVMAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYI 410
GSG V A I++G ++ QI+++++ V + P L+I+ VG+ S+ Y+
Sbjct: 129 GSG---VRHDAVIISGTEMAKQIQKEIQQGVVSWIS-LGNRRPHLSIILVGDNPASHTYV 184
Query: 411 NVKLKAAKEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEA 590
K+KAA +GI + I P+ ++ E+L LN D V G ++QLPL ++ A
Sbjct: 185 TKKIKAATAVGIYSEIILKPKDVSQEELLDITDQLNMDPRVSGILVQLPLPDH--VDERA 242
Query: 591 LINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGR 770
+ NA+APEKDVDG IN G+L IP T E+IK+TG++ G+ VV GR
Sbjct: 243 VCNAVAPEKDVDGFHIINIGRLCLD--QHSLIPATASAVWEIIKRTGIETFGKNVVVAGR 300
Query: 771 SKIVGAPMHDLL 806
SK VG P+ LL
Sbjct: 301 SKNVGMPIAMLL 312
>ref|XP_003361784.1| PREDICTED: bifunctional protein FolD-like, partial [Sus scrofa].
Length = 169
Score = 50.4 bits (119), Expect = 3e-06
Identities = 27/67 (40%), Positives = 42/67 (62%)
Frame = +3
Query: 429 AKEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIA 608
A + G+ HI LP ++E E++ I +NED VHG LQ+ SE +++ +++A+
Sbjct: 44 AFQAGLNITHICLPADSSEDEIIDEILKINEDSRVHGLALQI---SETSFSSK-ILHALK 99
Query: 609 PEKDVDG 629
PEKDVDG
Sbjct: 100 PEKDVDG 106
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 46,691,359
Number of extensions: 1336229
Number of successful extensions: 4356
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 4305
Number of HSP's successfully gapped: 3
Length of query: 591
Length of database: 11,343,932
Length adjustment: 105
Effective length of query: 486
Effective length of database: 8,729,747
Effective search space: 4242657042
Effective search space used: 4242657042
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008727
(1773 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_620084.2| C-1-tetrahydrofolate synthase, cytoplasmic [Mus... 834 0.0
Alignment gi|NP_001164257.1| monofunctional C1-tetrahydrofolate synthase,... 373 e-103
Alignment gi|NP_001164256.1| monofunctional C1-tetrahydrofolate synthase,... 373 e-103
Alignment gi|NP_758512.3| monofunctional C1-tetrahydrofolate synthase, mi... 373 e-103
Alignment gi|NP_032664.1| bifunctional methylenetetrahydrofolate dehydrog... 195 8e-50
Alignment gi|NP_081064.1| methylenetetrahydrofolate dehydrogenase 2-like ... 183 4e-46
>ref|NP_620084.2| C-1-tetrahydrofolate synthase, cytoplasmic [Mus musculus].
Length = 935
Score = 834 bits (2155), Expect = 0.0
Identities = 415/493 (84%), Positives = 450/493 (91%)
Frame = +3
Query: 252 MAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAA 431
MAPA ILNGK+VSAQIR++LK++V +M+EQVPGFTPGLAILQVG+RDDSNLYINVKLKAA
Sbjct: 1 MAPAGILNGKLVSAQIRDRLKNQVTRMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAA 60
Query: 432 KEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAP 611
+EIGIKA HIKLPRT+TESEVLK + SLNED +VHGFI+QLPLDSEN IN EA+INAIAP
Sbjct: 61 EEIGIKATHIKLPRTSTESEVLKYVISLNEDASVHGFIVQLPLDSENSINTEAVINAIAP 120
Query: 612 EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAP 791
EKDVDGLTS++AGKLARGDLNDCFIPCTPKGCLELIK+ GVQIAGR AVV+GRSKIVGAP
Sbjct: 121 EKDVDGLTSVSAGKLARGDLNDCFIPCTPKGCLELIKEAGVQIAGRHAVVVGRSKIVGAP 180
Query: 792 MHDLLLWNHATVTTCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINC 971
MHDLLLWN+ATVTTCHSKTANL++EVNKGDI+VVA G+PEMVKGEWIKPGAVVIDCGIN
Sbjct: 181 MHDLLLWNNATVTTCHSKTANLDKEVNKGDILVVATGQPEMVKGEWIKPGAVVIDCGINY 240
Query: 972 VPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRP 1151
VPDDTKP+G+++VGDVAY EAKERA FITPVPGGVGPMTVAMLMQSTVESA+ FL+KF+P
Sbjct: 241 VPDDTKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAQRFLQKFKP 300
Query: 1152 GKWIIQYNKLNLKTPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSL 1331
GKW IQYNKLNLKTPVPSDI ISRSCKPK IGNLAREIGLL+EEVELYGETKAKVLLS+L
Sbjct: 301 GKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGETKAKVLLSAL 360
Query: 1332 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI 1511
+RLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHL QNVFACVRQPSQGPTFGI
Sbjct: 361 DRLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLRQNVFACVRQPSQGPTFGI 420
Query: 1512 KXXXXXXXYSQVIPMEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLV 1691
K YSQVIPMEEFNLHLTGDIH R+FHE TQTDKALF RLV
Sbjct: 421 KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLV 480
Query: 1692 PSVNGVRKFSDIQ 1730
PSVNG+RKFSDIQ
Sbjct: 481 PSVNGIRKFSDIQ 493
>ref|NP_001164257.1| monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
[Mus musculus].
Length = 977
Score = 373 bits (958), Expect = e-103
Identities = 217/532 (40%), Positives = 306/532 (57%), Gaps = 16/532 (3%)
Frame = +3
Query: 183 CRVRESRPSILSVSTLGSGLTKVMAPAEILNGKVVSAQ-------------IREKL---K 314
CR+RE S S+ G G + + + +G+ S+ +R+ + K
Sbjct: 23 CRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSK 82
Query: 315 SKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPRTATESEV 494
++ ++E+ P F P L ++Q G D NL ++ AKE G+ HI LP + E E+
Sbjct: 83 EVLSLLQEKNPAFKPVLVVIQAG---DDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEI 139
Query: 495 LKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGKLARGDLN 674
+ I +NED VHG LQ+ DS + ++NA+ PEKDVDG+T IN GKL RGD
Sbjct: 140 IDEILKINEDPRVHGLTLQISEDSLS----NKVLNALKPEKDVDGVTDINLGKLVRGDAP 195
Query: 675 DCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTTCHSKTAN 854
+CF+ K +EL++++G+ + G++ +V+G + A + L + +C T
Sbjct: 196 ECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQ 255
Query: 855 LNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVGDVAYSEA 1034
L +++ + DIVV+ + KPE + WI G +++C + + + D +E
Sbjct: 256 LPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEE 315
Query: 1035 KERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLKTPVPSDID 1214
V + A+ +Q+ V S +L + + +W + KL +PVPSDI+
Sbjct: 316 S------------VSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIE 363
Query: 1215 ISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVVVTGITPTP 1394
ISR PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+V GITPTP
Sbjct: 364 ISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTP 423
Query: 1395 LGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIPMEEFNLH 1574
LGEGKST TIGLVQAL AHL N FAC+RQPSQGPTFG+K Y+QVIPMEEFNLH
Sbjct: 424 LGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLH 483
Query: 1575 LTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
LTGDIH R+ HE TQTDKAL+ RLVP VNGVR+FS+IQ
Sbjct: 484 LTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQ 535
>ref|NP_001164256.1| monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
[Mus musculus].
Length = 977
Score = 373 bits (958), Expect = e-103
Identities = 217/532 (40%), Positives = 306/532 (57%), Gaps = 16/532 (3%)
Frame = +3
Query: 183 CRVRESRPSILSVSTLGSGLTKVMAPAEILNGKVVSAQ-------------IREKL---K 314
CR+RE S S+ G G + + + +G+ S+ +R+ + K
Sbjct: 23 CRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSK 82
Query: 315 SKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPRTATESEV 494
++ ++E+ P F P L ++Q G D NL ++ AKE G+ HI LP + E E+
Sbjct: 83 EVLSLLQEKNPAFKPVLVVIQAG---DDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEI 139
Query: 495 LKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGKLARGDLN 674
+ I +NED VHG LQ+ DS + ++NA+ PEKDVDG+T IN GKL RGD
Sbjct: 140 IDEILKINEDPRVHGLTLQISEDSLS----NKVLNALKPEKDVDGVTDINLGKLVRGDAP 195
Query: 675 DCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTTCHSKTAN 854
+CF+ K +EL++++G+ + G++ +V+G + A + L + +C T
Sbjct: 196 ECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQ 255
Query: 855 LNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVGDVAYSEA 1034
L +++ + DIVV+ + KPE + WI G +++C + + + D +E
Sbjct: 256 LPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEE 315
Query: 1035 KERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLKTPVPSDID 1214
V + A+ +Q+ V S +L + + +W + KL +PVPSDI+
Sbjct: 316 S------------VSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIE 363
Query: 1215 ISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVVVTGITPTP 1394
ISR PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+V GITPTP
Sbjct: 364 ISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTP 423
Query: 1395 LGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIPMEEFNLH 1574
LGEGKST TIGLVQAL AHL N FAC+RQPSQGPTFG+K Y+QVIPMEEFNLH
Sbjct: 424 LGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLH 483
Query: 1575 LTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
LTGDIH R+ HE TQTDKAL+ RLVP VNGVR+FS+IQ
Sbjct: 484 LTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQ 535
>ref|NP_758512.3| monofunctional C1-tetrahydrofolate synthase, mitochondrial precursor
[Mus musculus].
Length = 977
Score = 373 bits (958), Expect = e-103
Identities = 217/532 (40%), Positives = 306/532 (57%), Gaps = 16/532 (3%)
Frame = +3
Query: 183 CRVRESRPSILSVSTLGSGLTKVMAPAEILNGKVVSAQ-------------IREKL---K 314
CR+RE S S+ G G + + + +G+ S+ +R+ + K
Sbjct: 23 CRLRELCRSGSRSSSSGGGDPEGLRGRRLQDGQTFSSHGPGNPEAPGMDSIVRDVIHNSK 82
Query: 315 SKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPRTATESEV 494
++ ++E+ P F P L ++Q G D NL ++ AKE G+ HI LP + E E+
Sbjct: 83 EVLSLLQEKNPAFKPVLVVIQAG---DDNLMKDMNQNLAKEAGLDITHICLPPDSGEDEI 139
Query: 495 LKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGKLARGDLN 674
+ I +NED VHG LQ+ DS + ++NA+ PEKDVDG+T IN GKL RGD
Sbjct: 140 IDEILKINEDPRVHGLTLQISEDSLS----NKVLNALKPEKDVDGVTDINLGKLVRGDAP 195
Query: 675 DCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTTCHSKTAN 854
+CF+ K +EL++++G+ + G++ +V+G + A + L + +C T
Sbjct: 196 ECFVSPLAKAAVELVEKSGITLDGKKVLVVGAHGPLEAALQWLFQRKGSMTMSCPWATPQ 255
Query: 855 LNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVGDVAYSEA 1034
L +++ + DIVV+ + KPE + WI G +++C + + + D +E
Sbjct: 256 LPDKLREADIVVLGSPKPEEIPAVWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAEE 315
Query: 1035 KERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLKTPVPSDID 1214
V + A+ +Q+ V S +L + + +W + KL +PVPSDI+
Sbjct: 316 S------------VSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIE 363
Query: 1215 ISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVVVTGITPTP 1394
ISR PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+V GITPTP
Sbjct: 364 ISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTP 423
Query: 1395 LGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIPMEEFNLH 1574
LGEGKST TIGLVQAL AHL N FAC+RQPSQGPTFG+K Y+QVIPMEEFNLH
Sbjct: 424 LGEGKSTVTIGLVQALTAHLKVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLH 483
Query: 1575 LTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
LTGDIH R+ HE TQTDKAL+ RLVP VNGVR+FS+IQ
Sbjct: 484 LTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPLVNGVREFSEIQ 535
>ref|NP_032664.1| bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase,
mitochondrial [Mus musculus].
Length = 350
Score = 195 bits (496), Expect = 8e-50
Identities = 121/309 (39%), Positives = 178/309 (57%), Gaps = 10/309 (3%)
Frame = +3
Query: 240 LTKVMAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVK 419
L V A +++G+ ++ QI+++++ +V + P L+++ VG+ S+ Y+ K
Sbjct: 30 LAAVRNEAVVISGRKLAQQIKQEVQQEVEEWVAS-GNKRPHLSVILVGDNPASHSYVLNK 88
Query: 420 LKAAKEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALIN 599
+AA E+GI + I P + +E E+L I LN D V G ++QLPL I+ + N
Sbjct: 89 TRAAAEVGINSETIVKPASVSEEELLNSIRKLNNDENVDGLLVQLPLPEH--IDERKVCN 146
Query: 600 AIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKI 779
A++P+KDVDG IN G++ + +P TP G E+IK+TG+ G+ VV GRSK
Sbjct: 147 AVSPDKDVDGFHVINVGRMCLDQYS--MLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKN 204
Query: 780 VGAPMHDLLLWN--------HATVTTCHSKTAN--LNEEVNKGDIVVVAAGKPEMVKGEW 929
VG P+ LL + ATVT H T L + DIV+ AAG P ++ +
Sbjct: 205 VGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADM 264
Query: 930 IKPGAVVIDCGINCVPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQS 1109
IK GA VID GIN V D K +VGDV + K++AG+ITPVPGGVGPMTVAMLM++
Sbjct: 265 IKEGAAVIDVGINRVQDPVTAKPK-LVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKN 323
Query: 1110 TVESAEHFL 1136
T+ +A+ L
Sbjct: 324 TIIAAKKVL 332
>ref|NP_081064.1| methylenetetrahydrofolate dehydrogenase 2-like [Mus musculus].
Length = 338
Score = 183 bits (464), Expect = 4e-46
Identities = 118/320 (36%), Positives = 178/320 (55%), Gaps = 11/320 (3%)
Frame = +3
Query: 204 PSILSVSTLGSGLTKVMAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVG 383
P + + G G + A I++G ++ QI+++++ V + P L+I+ VG
Sbjct: 23 PGVAERAWRGFGSSGRRHEAVIISGTNMAKQIQKEIQQGVESWIA-LGNRRPHLSIILVG 81
Query: 384 NRDDSNLYINVKLKAAKEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLD 563
+ S+ Y+ K+KAA +GI + I P+ ++ E+L LN D V G ++QLPL
Sbjct: 82 DNPASHTYVRNKIKAASAVGICSELIIKPKNVSQEELLDITDQLNMDPRVSGILVQLPLP 141
Query: 564 SENPINAEALINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIA 743
++ + N IAPEKDVDG IN G+L + IP T E+IK+ G++
Sbjct: 142 DH--VDERTICNGIAPEKDVDGFHIINIGRLCLDQHS--LIPATASAVWEIIKRAGIETF 197
Query: 744 GRQAVVIGRSKIVGAPMHDLLLWN--------HATVTTCHSKTAN--LNEEVNKGDIVVV 893
G+ VV GRSK VG P+ LL + ATVT H T L DI++V
Sbjct: 198 GKNVVVAGRSKNVGMPIAMLLHTDGEHERPGGDATVTIAHRYTPREQLKAHTQLADIIIV 257
Query: 894 AAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGK-RIVGDVAYSEAKERAGFITPVPG 1070
AAG P ++ + ++ GA VID GIN + D +GK ++VGDV + K++A FITPVPG
Sbjct: 258 AAGIPRLITADMVREGAAVIDVGINYIQDPV--TGKTKLVGDVDFEAVKKKASFITPVPG 315
Query: 1071 GVGPMTVAMLMQSTVESAEH 1130
GVGPMTVAML+++T+ +A++
Sbjct: 316 GVGPMTVAMLLKNTLLAAKN 335
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 62,923,891
Number of extensions: 1767972
Number of successful extensions: 5913
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 5848
Number of HSP's successfully gapped: 6
Length of query: 591
Length of database: 15,617,559
Length adjustment: 107
Effective length of query: 484
Effective length of database: 12,403,707
Effective search space: 6003394188
Effective search space used: 6003394188
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008727
(1773 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_005947.3| C-1-tetrahydrofolate synthase, cytoplasmic [Hom... 839 0.0
Alignment gi|NP_056255.2| monofunctional C1-tetrahydrofolate synthase, mi... 380 e-105
Alignment gi|NP_001229697.1| monofunctional C1-tetrahydrofolate synthase,... 377 e-104
Alignment gi|NP_001229696.1| monofunctional C1-tetrahydrofolate synthase,... 377 e-104
Alignment gi|NP_006627.2| bifunctional methylenetetrahydrofolate dehydrog... 192 1e-48
Alignment gi|NP_001138450.1| probable bifunctional methylenetetrahydrofol... 188 2e-47
Alignment gi|NP_001229698.1| monofunctional C1-tetrahydrofolate synthase,... 120 4e-27
>ref|NP_005947.3| C-1-tetrahydrofolate synthase, cytoplasmic [Homo sapiens].
Length = 935
Score = 839 bits (2167), Expect = 0.0
Identities = 418/493 (84%), Positives = 448/493 (90%)
Frame = +3
Query: 252 MAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAA 431
MAPAEILNGK +SAQIR +LK++V Q+KEQVPGFTP LAILQVGNRDDSNLYINVKLKAA
Sbjct: 1 MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAA 60
Query: 432 KEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAP 611
+EIGIKA HIKLPRT TESEV+K ITSLNED TVHGF++QLPLDSEN IN E +INAIAP
Sbjct: 61 EEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAP 120
Query: 612 EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAP 791
EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIK+TGV IAGR AVV+GRSKIVGAP
Sbjct: 121 EKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAP 180
Query: 792 MHDLLLWNHATVTTCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINC 971
MHDLLLWN+ATVTTCHSKTA+L+EEVNKGDI+VVA G+PEMVKGEWIKPGA+VIDCGIN
Sbjct: 181 MHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINY 240
Query: 972 VPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRP 1151
VPDD KP+G+++VGDVAY EAKERA FITPVPGGVGPMTVAMLMQSTVESA+ FLEKF+P
Sbjct: 241 VPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKP 300
Query: 1152 GKWIIQYNKLNLKTPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSL 1331
GKW+IQYN LNLKTPVPSDIDISRSCKPKPIG LAREIGLLSEEVELYGETKAKVLLS+L
Sbjct: 301 GKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSAL 360
Query: 1332 ERLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGI 1511
ERLKH+PDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHL+QNVFACVRQPSQGPTFGI
Sbjct: 361 ERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGI 420
Query: 1512 KXXXXXXXYSQVIPMEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLV 1691
K YSQVIPMEEFNLHLTGDIH R+FHE TQTDKALF RLV
Sbjct: 421 KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLV 480
Query: 1692 PSVNGVRKFSDIQ 1730
PSVNGVR+FSDIQ
Sbjct: 481 PSVNGVRRFSDIQ 493
>ref|NP_056255.2| monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 2
precursor [Homo sapiens].
Length = 978
Score = 380 bits (977), Expect = e-105
Identities = 216/479 (45%), Positives = 298/479 (62%)
Frame = +3
Query: 294 QIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPR 473
++ + K ++ ++E+ P F P LAI+Q G D NL + A+E G+ HI LP
Sbjct: 77 EVIQNSKEVLSLLQEKNPAFKPVLAIIQAG---DDNLMQEINQNLAEEAGLNITHICLPP 133
Query: 474 TATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGK 653
++E+E++ I +NED VHG LQ+ SEN + + ++NA+ PEKDVDG+T IN GK
Sbjct: 134 DSSEAEIIDEILKINEDTRVHGLALQI---SEN-LFSNKVLNALKPEKDVDGVTDINLGK 189
Query: 654 LARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTT 833
L RGD ++CF+ K +EL++++GV + G++ +V+G + A + L + +
Sbjct: 190 LVRGDAHECFVSPVAKAVIELLEKSGVNLDGKKILVVGAHGSLEAALQCLFQRKGSMTMS 249
Query: 834 CHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIVG 1013
KT L ++++ DIVV+ + KPE + WI+PG V++C + + SGK G
Sbjct: 250 IQWKTRQLQSKLHEADIVVLGSPKPEEIPLTWIQPGTTVLNCSHDFL------SGKVGCG 303
Query: 1014 DVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLKT 1193
S G I V + A+ +Q+ V S +L + + +W + KL +
Sbjct: 304 ----SPRIHFGGLIEE--DDVILLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLS 357
Query: 1194 PVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVVV 1373
PVPSDI+ISR PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+V
Sbjct: 358 PVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVRLSVLERLKDQADGKYVLV 417
Query: 1374 TGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVIP 1553
GITPTPLGEGKST TIGLVQAL AHL+ N FAC+RQPSQGPTFG+K Y+QVIP
Sbjct: 418 AGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIP 477
Query: 1554 MEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
MEEFNLHLTGDIH R+ HE TQTDKAL+ RLVP VNGVR+FS+IQ
Sbjct: 478 MEEFNLHLTGDIHAITAANNLLAAAIDTRILHENTQTDKALYNRLVPLVNGVREFSEIQ 536
>ref|NP_001229697.1| monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 3
[Homo sapiens].
Length = 913
Score = 377 bits (967), Expect = e-104
Identities = 217/480 (45%), Positives = 296/480 (61%), Gaps = 1/480 (0%)
Frame = +3
Query: 294 QIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPR 473
++ + K ++ ++E+ P F P LAI+Q G D NL + A+E G+ HI LP
Sbjct: 11 EVIQNSKEVLSLLQEKNPAFKPVLAIIQAG---DDNLMQEINQNLAEEAGLNITHICLPP 67
Query: 474 TATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGK 653
++E+E++ I +NED VHG LQ+ SEN + + ++NA+ PEKDVDG+T IN GK
Sbjct: 68 DSSEAEIIDEILKINEDTRVHGLALQI---SEN-LFSNKVLNALKPEKDVDGVTDINLGK 123
Query: 654 LARGDLNDCFIPCTPKGCLELI-KQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVT 830
L RGD ++CF+ K +EL+ K GV + G++ +V+G + A + L +
Sbjct: 124 LVRGDAHECFVSPVAKAVIELLEKSVGVNLDGKKILVVGAHGSLEAALQCLFQRKGSMTM 183
Query: 831 TCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIV 1010
+ KT L ++++ DIVV+ + KPE + WI+PG V++C + + SGK
Sbjct: 184 SIQWKTRQLQSKLHEADIVVLGSPKPEEIPLTWIQPGTTVLNCSHDFL------SGKVGC 237
Query: 1011 GDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLK 1190
G S G I V + A+ +Q+ V S +L + + +W + KL
Sbjct: 238 G----SPRIHFGGLIEE--DDVILLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPL 291
Query: 1191 TPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVV 1370
+PVPSDI+ISR PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+
Sbjct: 292 SPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVRLSVLERLKDQADGKYVL 351
Query: 1371 VTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVI 1550
V GITPTPLGEGKST TIGLVQAL AHL+ N FAC+RQPSQGPTFG+K Y+QVI
Sbjct: 352 VAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVI 411
Query: 1551 PMEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
PMEEFNLHLTGDIH R+ HE TQTDKAL+ RLVP VNGVR+FS+IQ
Sbjct: 412 PMEEFNLHLTGDIHAITAANNLLAAAIDTRILHENTQTDKALYNRLVPLVNGVREFSEIQ 471
>ref|NP_001229696.1| monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform 1
precursor [Homo sapiens].
Length = 979
Score = 377 bits (967), Expect = e-104
Identities = 217/480 (45%), Positives = 296/480 (61%), Gaps = 1/480 (0%)
Frame = +3
Query: 294 QIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPR 473
++ + K ++ ++E+ P F P LAI+Q G D NL + A+E G+ HI LP
Sbjct: 77 EVIQNSKEVLSLLQEKNPAFKPVLAIIQAG---DDNLMQEINQNLAEEAGLNITHICLPP 133
Query: 474 TATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGK 653
++E+E++ I +NED VHG LQ+ SEN + + ++NA+ PEKDVDG+T IN GK
Sbjct: 134 DSSEAEIIDEILKINEDTRVHGLALQI---SEN-LFSNKVLNALKPEKDVDGVTDINLGK 189
Query: 654 LARGDLNDCFIPCTPKGCLELI-KQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVT 830
L RGD ++CF+ K +EL+ K GV + G++ +V+G + A + L +
Sbjct: 190 LVRGDAHECFVSPVAKAVIELLEKSVGVNLDGKKILVVGAHGSLEAALQCLFQRKGSMTM 249
Query: 831 TCHSKTANLNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGKRIV 1010
+ KT L ++++ DIVV+ + KPE + WI+PG V++C + + SGK
Sbjct: 250 SIQWKTRQLQSKLHEADIVVLGSPKPEEIPLTWIQPGTTVLNCSHDFL------SGKVGC 303
Query: 1011 GDVAYSEAKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFLEKFRPGKWIIQYNKLNLK 1190
G S G I V + A+ +Q+ V S +L + + +W + KL
Sbjct: 304 G----SPRIHFGGLIEE--DDVILLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPL 357
Query: 1191 TPVPSDIDISRSCKPKPIGNLAREIGLLSEEVELYGETKAKVLLSSLERLKHQPDGKYVV 1370
+PVPSDI+ISR PK + LA+EIGLL++E+E+YG++KAKV LS LERLK Q DGKYV+
Sbjct: 358 SPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVRLSVLERLKDQADGKYVL 417
Query: 1371 VTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKXXXXXXXYSQVI 1550
V GITPTPLGEGKST TIGLVQAL AHL+ N FAC+RQPSQGPTFG+K Y+QVI
Sbjct: 418 VAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVI 477
Query: 1551 PMEEFNLHLTGDIHXXXXXXXXXXXXXXXRMFHEQTQTDKALFYRLVPSVNGVRKFSDIQ 1730
PMEEFNLHLTGDIH R+ HE TQTDKAL+ RLVP VNGVR+FS+IQ
Sbjct: 478 PMEEFNLHLTGDIHAITAANNLLAAAIDTRILHENTQTDKALYNRLVPLVNGVREFSEIQ 537
>ref|NP_006627.2| bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase,
mitochondrial precursor [Homo sapiens].
Length = 350
Score = 192 bits (487), Expect = 1e-48
Identities = 119/309 (38%), Positives = 176/309 (56%), Gaps = 10/309 (3%)
Frame = +3
Query: 240 LTKVMAPAEILNGKVVSAQIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVK 419
L V A +++G+ ++ QI+++++ +V + P L+++ VG S+ Y+ K
Sbjct: 30 LAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVAS-GNKRPHLSVILVGENPASHSYVLNK 88
Query: 420 LKAAKEIGIKAMHIKLPRTATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALIN 599
+AA +GI + I P + +E E+L I LN D V G ++QLPL I+ + N
Sbjct: 89 TRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEH--IDERRICN 146
Query: 600 AIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKI 779
A++P+KDVDG IN G++ + +P TP G E+IK+TG+ G+ VV GRSK
Sbjct: 147 AVSPDKDVDGFHVINVGRMCLDQYS--MLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKN 204
Query: 780 VGAPMHDLLLWN--------HATVTTCHSKTAN--LNEEVNKGDIVVVAAGKPEMVKGEW 929
VG P+ LL + ATVT H T L + DIV+ AAG P ++ +
Sbjct: 205 VGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADM 264
Query: 930 IKPGAVVIDCGINCVPDDTKPSGKRIVGDVAYSEAKERAGFITPVPGGVGPMTVAMLMQS 1109
IK GA VID GIN V D K +VGDV + +++AG+ITPVPGGVGPMTVAMLM++
Sbjct: 265 IKEGAAVIDVGINRVHDPVTAKPK-LVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKN 323
Query: 1110 TVESAEHFL 1136
T+ +A+ L
Sbjct: 324 TIIAAKKVL 332
>ref|NP_001138450.1| probable bifunctional methylenetetrahydrofolate
dehydrogenase/cyclohydrolase 2 [Homo sapiens].
Length = 347
Score = 188 bits (477), Expect = 2e-47
Identities = 125/335 (37%), Positives = 185/335 (55%), Gaps = 16/335 (4%)
Frame = +3
Query: 180 PCRVRESRPSILSVSTLGSGL-----TKVMAPAEILNGKVVSAQIREKLKSKVAQMKEQV 344
P R + PS+ + GS + V A I++G ++ I+++++ V +
Sbjct: 19 PALGRSTAPSVRAPGEPGSAFRGFRSSGVRHEAIIISGTEMAKHIQKEIQRGVESWVS-L 77
Query: 345 PGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPRTATESEVLKCITSLNED 524
P L+I+ VG+ S+ Y+ K++AA +GI + I P+ ++ E+L LN D
Sbjct: 78 GNRRPHLSIILVGDNPASHTYVRNKIRAASAVGICSELILKPKDVSQEELLDVTDQLNMD 137
Query: 525 LTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKG 704
V G ++QLPL ++ + N IAPEKDVDG IN G+L + IP T
Sbjct: 138 PRVSGILVQLPLPDH--VDERTICNGIAPEKDVDGFHIINIGRLCLDQHS--LIPATASA 193
Query: 705 CLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWN--------HATVTTCHSKTAN-- 854
E+IK+TG+Q G+ VV GRSK VG P+ LL + ATVT H T
Sbjct: 194 VWEIIKRTGIQTFGKNVVVAGRSKNVGMPIAMLLHTDGEHERPGGDATVTIAHRYTPKEQ 253
Query: 855 LNEEVNKGDIVVVAAGKPEMVKGEWIKPGAVVIDCGINCVPDDTKPSGK-RIVGDVAYSE 1031
L DI++VAAG P+++ + +K GA VID GIN V D +GK ++VGDV +
Sbjct: 254 LKIHTQLADIIIVAAGIPKLITSDMVKEGAAVIDVGINYVHDPV--TGKTKLVGDVDFEA 311
Query: 1032 AKERAGFITPVPGGVGPMTVAMLMQSTVESAEHFL 1136
K++AGFITPVPGGVGPMTVAML+++T+ +A+ +
Sbjct: 312 VKKKAGFITPVPGGVGPMTVAMLLKNTLLAAKKII 346
>ref|NP_001229698.1| monofunctional C1-tetrahydrofolate synthase, mitochondrial isoform
4 precursor [Homo sapiens].
Length = 275
Score = 120 bits (301), Expect = 4e-27
Identities = 67/197 (34%), Positives = 111/197 (56%)
Frame = +3
Query: 294 QIREKLKSKVAQMKEQVPGFTPGLAILQVGNRDDSNLYINVKLKAAKEIGIKAMHIKLPR 473
++ + K ++ ++E+ P F P LAI+Q G D NL + A+E G+ HI LP
Sbjct: 77 EVIQNSKEVLSLLQEKNPAFKPVLAIIQAG---DDNLMQEINQNLAEEAGLNITHICLPP 133
Query: 474 TATESEVLKCITSLNEDLTVHGFILQLPLDSENPINAEALINAIAPEKDVDGLTSINAGK 653
++E+E++ I +NED VHG LQ+ SEN + + ++NA+ PEKDVDG+T IN GK
Sbjct: 134 DSSEAEIIDEILKINEDTRVHGLALQI---SEN-LFSNKVLNALKPEKDVDGVTDINLGK 189
Query: 654 LARGDLNDCFIPCTPKGCLELIKQTGVQIAGRQAVVIGRSKIVGAPMHDLLLWNHATVTT 833
L RGD ++CF+ K +EL++++GV + G++ +V+G + A + L + +
Sbjct: 190 LVRGDAHECFVSPVAKAVIELLEKSGVNLDGKKILVVGAHGSLEAALQCLFQRKGSMTMS 249
Query: 834 CHSKTANLNEEVNKGDI 884
KT L + +
Sbjct: 250 IQWKTRQLQSKTESRSV 266
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 74,185,696
Number of extensions: 2107309
Number of successful extensions: 6676
Number of sequences better than 1.0e-05: 7
Number of HSP's gapped: 6578
Number of HSP's successfully gapped: 7
Length of query: 591
Length of database: 18,297,164
Length adjustment: 108
Effective length of query: 483
Effective length of database: 14,737,052
Effective search space: 7117996116
Effective search space used: 7117996116
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008727
(1773 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_Chr07 504 e-140
>Sscrofa_Chr07
|| Length = 134764511
Score = 504 bits (254), Expect = e-140
Identities = 284/296 (95%), Gaps = 3/296 (1%)
Strand = Plus / Plus
Query: 1 gtctacacagcgttccctcatcttcctgcggcttttcagaccccgtatgccgcagttctt 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94832631 gtctacacagcgttccctcatcttcctgcggcttttcagaccccgtatgccgcagttctt 94832690
Query: 61 cgctacccctggggcgtacgtccgtctgggccgcgcatgcgcatccacgtcctnnnnnnn 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94832691 cgctacccctggggcgtacgtccgtctgggccgcgcatgcgcatccacgtcctggggggg 94832750
Query: 121 n---agcaggcgcaatagtagggtcagcctgggaggtcgctaagcacgtgtgttgggctg 177
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94832751 ggcgagcaggcgcaatagtagggtcagcctgggaggtcgctaagcacgtgtgttgggctc 94832810
Query: 178 tcccgtgccgcgttagagagagtcgaccctcgattttatcggtgtccactttgggcagcg 237
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94832811 tcccgtgccgcgttagagagagtcgaccctcgattttatcggtgtccactttgggcagcg 94832870
Query: 238 gacttacaaaggtcatggcgccggcggaaatcctgaacgggaaggtggtctccgcg 293
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94832871 gacttacaaaggtcatggcgccggcggaaatcctgaacgggaaggtggtctccgcg 94832926
Score = 351 bits (177), Expect = 8e-94
Identities = 177/177 (100%)
Strand = Plus / Plus
Query: 1202 aagtgacattgatatatcacggtcttgcaagccaaagcccattggtaacctggctcgaga 1261
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94866346 aagtgacattgatatatcacggtcttgcaagccaaagcccattggtaacctggctcgaga 94866405
Query: 1262 aattggtctgctctctgaagaggtagaattatatggtgaaaccaaggccaaggtcctgct 1321
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94866406 aattggtctgctctctgaagaggtagaattatatggtgaaaccaaggccaaggtcctgct 94866465
Query: 1322 gtcgtccctggaacgcctgaagcaccagcctgacgggaaatatgtggtggtgactgg 1378
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94866466 gtcgtccctggaacgcctgaagcaccagcctgacgggaaatatgtggtggtgactgg 94866522
Score = 285 bits (144), Expect = 4e-74
Identities = 144/144 (100%)
Strand = Plus / Plus
Query: 727 caggggtgcagattgccgggaggcaggctgtggtgatcgggcgcagtaaaatagtgggtg 786
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94859089 caggggtgcagattgccgggaggcaggctgtggtgatcgggcgcagtaaaatagtgggtg 94859148
Query: 787 ccccgatgcatgacttgcttctgtggaaccatgccacagtgaccacctgccactccaaga 846
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94859149 ccccgatgcatgacttgcttctgtggaaccatgccacagtgaccacctgccactccaaga 94859208
Query: 847 ctgccaatctaaacgaggaggtaa 870
||||||||||||||||||||||||
Sbjct: 94859209 ctgccaatctaaacgaggaggtaa 94859232
Score = 278 bits (140), Expect = 1e-71
Identities = 140/140 (100%)
Strand = Plus / Plus
Query: 1378 gcataactccaactcccctgggagaggggaaaagcacaaccacgattgggttggtgcagg 1437
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94867448 gcataactccaactcccctgggagaggggaaaagcacaaccacgattgggttggtgcagg 94867507
Query: 1438 cccttggcgcccatctccatcagaacgtcttcgcgtgtgtgcgacagccttcccagggcc 1497
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94867508 cccttggcgcccatctccatcagaacgtcttcgcgtgtgtgcgacagccttcccagggcc 94867567
Query: 1498 ccacctttggaataaaaggt 1517
||||||||||||||||||||
Sbjct: 94867568 ccacctttggaataaaaggt 94867587
Score = 276 bits (139), Expect = 4e-71
Identities = 139/139 (100%)
Strand = Plus / Plus
Query: 490 aggtgttaaagtgcattacatctttaaacgaagacctcactgtacatgggttcatactgc 549
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94855785 aggtgttaaagtgcattacatctttaaacgaagacctcactgtacatgggttcatactgc 94855844
Query: 550 agctgcctttagattcagagaatcctattaacgcagaggcactgatcaatgctattgcac 609
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94855845 agctgcctttagattcagagaatcctattaacgcagaggcactgatcaatgctattgcac 94855904
Query: 610 ccgaaaaggatgtggacgg 628
|||||||||||||||||||
Sbjct: 94855905 ccgaaaaggatgtggacgg 94855923
Score = 262 bits (132), Expect = 6e-67
Identities = 132/132 (100%)
Strand = Plus / Plus
Query: 975 ccagatgatacaaaaccaagtgggaaaagaattgtgggagacgtggcatacagcgaggcc 1034
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94865203 ccagatgatacaaaaccaagtgggaaaagaattgtgggagacgtggcatacagcgaggcc 94865262
Query: 1035 aaggagagggcgggcttcatcactcctgttcctggtggcgttgggcccatgaccgtggcg 1094
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94865263 aaggagagggcgggcttcatcactcctgttcctggtggcgttgggcccatgaccgtggcg 94865322
Query: 1095 atgctaatgcag 1106
||||||||||||
Sbjct: 94865323 atgctaatgcag 94865334
Score = 226 bits (114), Expect = 3e-56
Identities = 114/114 (100%)
Strand = Plus / Plus
Query: 865 aggtaaataaaggtgacatcgtggtggttgcagctggtaagcccgaaatggtgaaagggg 924
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94860674 aggtaaataaaggtgacatcgtggtggttgcagctggtaagcccgaaatggtgaaagggg 94860733
Query: 925 agtggatcaagccaggagcggtagtcattgactgtggcatcaattgtgtcccag 978
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94860734 agtggatcaagccaggagcggtagtcattgactgtggcatcaattgtgtcccag 94860787
Score = 220 bits (111), Expect = 2e-54
Identities = 111/111 (100%)
Strand = Plus / Plus
Query: 1561 agtttaatctccacctcactggtgacatccatgccatcaccgcagctaataaccttgttg 1620
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94870074 agtttaatctccacctcactggtgacatccatgccatcaccgcagctaataaccttgttg 94870133
Query: 1621 ctgcagccatcgatgcccggatgttccatgaacagacccagacagacaagg 1671
|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94870134 ctgcagccatcgatgcccggatgttccatgaacagacccagacagacaagg 94870184
Score = 198 bits (100), Expect = 7e-48
Identities = 100/100 (100%)
Strand = Plus / Plus
Query: 1105 agagcacagtggagagtgcagagcatttcctggagaaatttaggccaggaaagtggatca 1164
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94866071 agagcacagtggagagtgcagagcatttcctggagaaatttaggccaggaaagtggatca 94866130
Query: 1165 ttcaatataacaaacttaacctcaagacacctgtgccaag 1204
||||||||||||||||||||||||||||||||||||||||
Sbjct: 94866131 ttcaatataacaaacttaacctcaagacacctgtgccaag 94866170
Score = 196 bits (99), Expect = 3e-47
Identities = 102/103 (99%)
Strand = Plus / Plus
Query: 628 gattgactagcatcaatgctgggaaacttgctagaggtgacctaaatgactgcttcatcc 687
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct: 94856040 gattgactagcatcaatgctgggaaacttgctagaggtgacctaaatgactgtttcatcc 94856099
Query: 688 cttgtacacccaaaggatgcttggaactcatcaagcagacagg 730
|||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94856100 cttgtacacccaaaggatgcttggaactcatcaagcagacagg 94856142
Score = 172 bits (87), Expect = 4e-40
Identities = 87/87 (100%)
Strand = Plus / Plus
Query: 293 gcaaataagggagaaactgaagagcaaagtcgctcagatgaaggagcaggtacctggttt 352
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94846986 gcaaataagggagaaactgaagagcaaagtcgctcagatgaaggagcaggtacctggttt 94847045
Query: 353 caccccaggcctggccattttacaggt 379
|||||||||||||||||||||||||||
Sbjct: 94847046 caccccaggcctggccattttacaggt 94847072
Score = 131 bits (66), Expect = 1e-27
Identities = 75/78 (96%)
Strand = Plus / Plus
Query: 1668 aaggctctcttttatcgtttggtgccatcagtaaatggagtgagaaagttctctgatatc 1727
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94870322 aaggctctctttaatcgtttggtgccatcagtaaatggagtgagaaagttctctgatatc 94870381
Query: 1728 caactccgggagctgtgg 1745
||||||||| |||||||
Sbjct: 94870382 caactccggaggctgtgg 94870399
Score = 123 bits (62), Expect = 4e-25
Identities = 62/62 (100%)
Strand = Plus / Plus
Query: 376 aggttggcaacagagatgattctaatctttatataaatgtgaaactgaaggctgctaaag 435
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94853632 aggttggcaacagagatgattctaatctttatataaatgtgaaactgaaggctgctaaag 94853691
Query: 436 ag 437
||
Sbjct: 94853692 ag 94853693
Score = 117 bits (59), Expect = 2e-23
Identities = 59/59 (100%)
Strand = Plus / Plus
Query: 436 agattggaatcaaagccatgcacattaagttaccacgaacggccactgaatctgaggtg 494
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94854566 agattggaatcaaagccatgcacattaagttaccacgaacggccactgaatctgaggtg 94854624
Score = 97.6 bits (49), Expect = 2e-17
Identities = 49/49 (100%)
Strand = Plus / Plus
Query: 1514 aggtggcgctgcaggcggtggctactcacaggtcattcctatggaagag 1562
|||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94869402 aggtggcgctgcaggcggtggctactcacaggtcattcctatggaagag 94869450
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 48,045,381
Number of extensions: 227
Number of successful extensions: 227
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 227
Number of HSP's successfully gapped: 16
Length of query: 1773
Length of database: 2,808,509,378
Length adjustment: 22
Effective length of query: 1751
Effective length of database: 2,808,408,574
Effective search space: 4917523413074
Effective search space used: 4917523413074
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)