Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012772
(1773 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_002694023.1| PREDICTED: AU RNA binding protein/enoyl-Coen... 261 2e-69
Alignment gi|NP_001098906.1| methylglutaconyl-CoA hydratase, mitochondria... 237 3e-62
Alignment gi|NP_001033625.1| enoyl-CoA hydratase domain-containing protei... 121 2e-27
Alignment gi|NP_001020377.2| enoyl-CoA hydratase, mitochondrial precursor... 50 6e-06
Alignment gi|NP_001069248.2| peroxisomal bifunctional enzyme [Bos taurus]. 50 8e-06
>ref|XP_002694023.1| PREDICTED: AU RNA binding protein/enoyl-Coenzyme A hydratase-like
[Bos taurus].
Length = 288
Score = 261 bits (666), Expect = 2e-69
Identities = 134/165 (81%), Positives = 145/165 (87%)
Frame = +2
Query: 50 AAAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGGAAW 229
AA+ PGALGTL+A RARLVAA C +G RLPGSWT P + WARGWTPAAG AAW
Sbjct: 4 AASAAPGALGTLQASRARLVAACCTRLGARCRLPGSWT-CPRVDSTWARGWTPAAGKAAW 62
Query: 230 RRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDKK 409
+RGYS+E KTEDELRV YLEEENRGIVVLGINRAYAKNSLSKNL+KMLS+AVDALKSDKK
Sbjct: 63 QRGYSAEVKTEDELRVWYLEEENRGIVVLGINRAYAKNSLSKNLIKMLSRAVDALKSDKK 122
Query: 410 VRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEI 544
VRTIIVR+EVPGIFCAGADLKER KM SSEVGPFVSK+RA+INEI
Sbjct: 123 VRTIIVRNEVPGIFCAGADLKERAKMSSSEVGPFVSKIRAMINEI 167
>ref|NP_001098906.1| methylglutaconyl-CoA hydratase, mitochondrial [Bos taurus].
Length = 161
Score = 237 bits (604), Expect = 3e-62
Identities = 119/143 (83%), Positives = 125/143 (87%)
Frame = +2
Query: 50 AAAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGGAAW 229
AA+ PGALGTL+A RARLVAA CA +GP WRLPGSWT V IWARGWTPAAGGAAW
Sbjct: 4 AASAAPGALGTLQASRARLVAACCARLGPRWRLPGSWTCPRVDSTIWARGWTPAAGGAAW 63
Query: 230 RRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDKK 409
+RGYS+E KTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNL+KMLSKAVDALKSDKK
Sbjct: 64 QRGYSAEVKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLIKMLSKAVDALKSDKK 123
Query: 410 VRTIIVRSEVPGIFCAGADLKER 478
VRTIIVRSEVPGIFCA K R
Sbjct: 124 VRTIIVRSEVPGIFCAETYCKRR 146
>ref|NP_001033625.1| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
precursor [Bos taurus].
Length = 296
Score = 121 bits (303), Expect = 2e-27
Identities = 72/169 (42%), Positives = 95/169 (56%), Gaps = 1/169 (0%)
Frame = +2
Query: 194 RGWTPAAGGAAWRRGYSSEAKTED-ELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKM 370
R W P RG SS E++V L N+GI + +NR A+N+L V
Sbjct: 13 RPWKPLE-----TRGCSSNPGAAGREIQVCALAGPNQGIAEILMNRPSARNALGNVFVSQ 67
Query: 371 LSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIAN 550
L +A+ L+ D++VR +I RS V G+FCAGADLKER +M +EVG FV +LR ++ EIA
Sbjct: 68 LLEALAQLREDRQVRVLIFRSGVKGVFCAGADLKEREQMSEAEVGLFVQRLRGLMTEIAA 127
Query: 551 LPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
P PTI CD+RVAASSA MGL+ET ++PG G
Sbjct: 128 FPAPTIAAMDGFALGGGLELALACDLRVAASSAVMGLIETTRGLLPGAG 176
>ref|NP_001020377.2| enoyl-CoA hydratase, mitochondrial precursor [Bos taurus].
Length = 290
Score = 50.1 bits (118), Expect = 6e-06
Identities = 42/158 (26%), Positives = 68/158 (43%), Gaps = 2/158 (1%)
Frame = +2
Query: 230 RRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDKK 409
+R ++S A E + + N G++ L NR A N+L L+ L++A+ A + D
Sbjct: 24 QRSFASSAAFEYIITAKKGRNSNVGLIQL--NRPKALNALCNGLIVELNQALQAFEEDPA 81
Query: 410 VRTIIVRSEVPGIFCAGADLKERVKM--HSSEVGPFVSKLRAVINEIANLPVPTIXXXXX 583
V I++ +F AGAD+KE + + G F+S +++ + P I
Sbjct: 82 VGAIVLTGGEK-VFAAGADIKEMQSLTFQNCYSGGFLSHW----DQLTRVKKPVIAAVNG 136
Query: 584 XXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
CDI A A+ G E + IPG G
Sbjct: 137 YALGGGCELAMMCDIIYAGEKAQFGQPEILIGTIPGAG 174
>ref|NP_001069248.2| peroxisomal bifunctional enzyme [Bos taurus].
Length = 723
Score = 49.7 bits (117), Expect = 8e-06
Identities = 31/117 (26%), Positives = 57/117 (48%)
Frame = +2
Query: 341 NSLSKNLVKMLSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSK 520
N++S + + + +++ +D V+ I++ GIFCAGAD++E K+H + F +
Sbjct: 22 NAISTTVARGIKESLQKAITDDTVKAIVICG-ADGIFCAGADIRE-FKVHKT----FDIQ 75
Query: 521 LRAVINEIANLPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPG 691
L +++EI P + C R+A + A++G E L I+PG
Sbjct: 76 LGDIVDEIQRNKKPVVAAIQKLALGGGLELALGCHYRIAHAEAQVGFPEVTLGILPG 132
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 46,140,438
Number of extensions: 1557197
Number of successful extensions: 3725
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 3713
Number of HSP's successfully gapped: 5
Length of query: 591
Length of database: 17,681,374
Length adjustment: 108
Effective length of query: 483
Effective length of database: 14,107,870
Effective search space: 6814101210
Effective search space used: 6814101210
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012772
(1773 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_533549.2| PREDICTED: similar to Methylglutaconyl-CoA hydr... 339 6e-93
Alignment gi|XP_853370.1| PREDICTED: similar to enoyl Coenzyme A hydratas... 114 4e-25
Alignment gi|XP_547179.2| PREDICTED: similar to 3,2-trans-enoyl-CoA isome... 52 2e-06
>ref|XP_533549.2| PREDICTED: similar to Methylglutaconyl-CoA hydratase, mitochondrial
precursor (AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding protein/enoyl-CoA hydratase) [Canis
familiaris].
Length = 340
Score = 339 bits (869), Expect = 6e-93
Identities = 179/218 (82%), Positives = 188/218 (86%), Gaps = 1/218 (0%)
Frame = +2
Query: 47 AAAAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGG-A 223
AAAA PGALGTL+AGRARLVAA CA +GPGWRLPGS TG V PA+WARGWTPAAGG +
Sbjct: 3 AAAAAAPGALGTLQAGRARLVAACCARLGPGWRLPGSVTGPRVAPAVWARGWTPAAGGGS 62
Query: 224 AWRRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSD 403
A RRGYSSE KTEDELRVRYLEEENRGIVVLGINRAYAKN+ SK+LVKMLSKAVDALKSD
Sbjct: 63 APRRGYSSEVKTEDELRVRYLEEENRGIVVLGINRAYAKNTFSKSLVKMLSKAVDALKSD 122
Query: 404 KKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIANLPVPTIXXXXX 583
KKVRTIIVRSEVPGIFCAGADLKERVKM+ SEVGPFVSK+RAVI+EIANLPVPTI
Sbjct: 123 KKVRTIIVRSEVPGIFCAGADLKERVKMNPSEVGPFVSKIRAVIDEIANLPVPTIAAIDG 182
Query: 584 XXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
CDIRVAASSAKMGLVETKLAIIPGGG
Sbjct: 183 LALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGG 220
>ref|XP_853370.1| PREDICTED: similar to enoyl Coenzyme A hydratase domain containing
2 [Canis familiaris].
Length = 147
Score = 114 bits (284), Expect = 4e-25
Identities = 60/126 (47%), Positives = 79/126 (62%)
Frame = +2
Query: 320 INRAYAKNSLSKNLVKMLSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSE 499
+NR A N+L LV L +A+ L+ D++VR ++ RS V G+FCAGADLKER +M +E
Sbjct: 1 MNRPSACNALGNVLVNQLLEALAQLREDQQVRVLLFRSGVKGVFCAGADLKEREQMSEAE 60
Query: 500 VGPFVSKLRAVINEIANLPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLA 679
VG FV +LR ++NEIA P PTI CD+RVAASSA MGL+ET
Sbjct: 61 VGIFVQRLRGLMNEIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSAVMGLIETTRG 120
Query: 680 IIPGGG 697
++PG G
Sbjct: 121 LLPGAG 126
>ref|XP_547179.2| PREDICTED: similar to 3,2-trans-enoyl-CoA isomerase, mitochondrial
precursor (Dodecenoyl-CoA delta-isomerase)
(D3,D2-enoyl-CoA isomerase) [Canis familiaris].
Length = 342
Score = 51.6 bits (122), Expect = 2e-06
Identities = 61/213 (28%), Positives = 89/213 (41%), Gaps = 21/213 (9%)
Frame = +2
Query: 110 AAYCAGIGPGWRLPG--SWTGLPVGPAIWAR------------GWTPAAGGAAWRRGYSS 247
AA G PG G +W P GP + A G TPAAGG
Sbjct: 25 AAVLPGYPPGPEAAGGRAWQAAP-GPPVAADSRRGSRVLSAAPGRTPAAGGGG------P 77
Query: 248 EAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDKKVRTIIV 427
A+ RV + G+ V+ + + NSLS L+ L +++ L++DK + +I+
Sbjct: 78 GARCFGSPRVLVEPDPAAGMAVMKLRKPPV-NSLSLELLTELVISLEKLENDKSFQGVII 136
Query: 428 RSEVPGIFCAGADLKE---RVKMHSSEVGPFVSK--LRAVINEIANLPVPTIXXXXXXXX 592
S+ PGIF AG DL E R H +E V + LR ++ + I
Sbjct: 137 TSDSPGIFSAGLDLMEMCGRDPAHYAEYWKAVQELWLRLYLSHLV-----LICAVNGASP 191
Query: 593 XXXXXXXXXCDIRVAASSAK--MGLVETKLAII 685
CD R+ A + K +GL ET+L I+
Sbjct: 192 AGGCLLALSCDYRILADNPKYTIGLNETQLGIV 224
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 45,118,494
Number of extensions: 1085771
Number of successful extensions: 3847
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 3825
Number of HSP's successfully gapped: 3
Length of query: 591
Length of database: 18,874,504
Length adjustment: 108
Effective length of query: 483
Effective length of database: 15,274,216
Effective search space: 7377446328
Effective search space used: 7377446328
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012772
(1773 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_001928827.3| PREDICTED: methylglutaconyl-CoA hydratase, m... 393 e-109
Alignment gi|XP_003128050.2| PREDICTED: enoyl-CoA hydratase domain-contai... 117 2e-26
Alignment gi|XP_003130864.2| PREDICTED: hypothetical protein LOC100513945... 51 2e-06
>ref|XP_001928827.3| PREDICTED: methylglutaconyl-CoA hydratase, mitochondrial [Sus
scrofa].
Length = 338
Score = 393 bits (1009), Expect = e-109
Identities = 201/218 (92%), Positives = 201/218 (92%)
Frame = +2
Query: 44 MAAAAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGGA 223
MAAAAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGGA
Sbjct: 1 MAAAAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGGA 60
Query: 224 AWRRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSD 403
AWRRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSD
Sbjct: 61 AWRRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSD 120
Query: 404 KKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIANLPVPTIXXXXX 583
KKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIANLPVPTI
Sbjct: 121 KKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIANLPVPTIAAIDG 180
Query: 584 XXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
CDIRVAASSAKMGLVETKLAIIPGGG
Sbjct: 181 LALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGG 218
>ref|XP_003128050.2| PREDICTED: enoyl-CoA hydratase domain-containing protein 2,
mitochondrial-like isoform 1 [Sus scrofa].
Length = 275
Score = 117 bits (293), Expect = 2e-26
Identities = 62/132 (46%), Positives = 81/132 (61%)
Frame = +2
Query: 302 GIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERV 481
GI + +NR A+N+L V L +A+ L+ D+ VR +I RS V G+FCAGADLKER
Sbjct: 24 GITEILMNRPSARNALGNVFVSQLLEALAQLREDRHVRVLIFRSGVKGVFCAGADLKERE 83
Query: 482 KMHSSEVGPFVSKLRAVINEIANLPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGL 661
+M +EVG FV +LR ++NEIA P PTI CD+RVAASSA MGL
Sbjct: 84 QMSEAEVGVFVQRLRGLMNEIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSAVMGL 143
Query: 662 VETKLAIIPGGG 697
+ET ++PG G
Sbjct: 144 IETTRGLLPGAG 155
>ref|XP_003130864.2| PREDICTED: hypothetical protein LOC100513945 [Sus scrofa].
Length = 845
Score = 51.2 bits (121), Expect = 2e-06
Identities = 64/238 (26%), Positives = 96/238 (40%), Gaps = 11/238 (4%)
Frame = +2
Query: 35 TENMAAAAGVPGALGTLRAGRARLVAAYCAGI------GPGWRLPGSWTGLPVGPAIWAR 196
+E AA GV LG +R A L A +G+ GPGW G W L
Sbjct: 523 SERQTAALGVE--LGGVRPPGASLAAMAASGLRALGAKGPGWLRRGPWAPL--------- 571
Query: 197 GWTPAAGGAAWRRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLS 376
AAG + RG + + E R+ + + GI + +N +N+LS ++K L
Sbjct: 572 ----AAGFCS--RGSAGVGRPEPGPRLTSARQRD-GIRSIVLNNPKRRNALSLAMLKSLQ 624
Query: 377 KAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGP--FVSKLRA---VINE 541
+ + ++ I++ +E P +F +G DLKE ++E GP RA V+
Sbjct: 625 SDLLHDAESRDLKVIVISAEGP-VFSSGHDLKEL----TAEQGPDYHAEVFRACSEVMML 679
Query: 542 IANLPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGGMNCSCP 715
I N PVP I CDI VA+ + + G+ CS P
Sbjct: 680 IQNHPVPIIAMVNGLATAAGCQLVASCDIAVASDKSSFATPGVTI------GLFCSTP 731
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 34,251,863
Number of extensions: 1795485
Number of successful extensions: 2484
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 2477
Number of HSP's successfully gapped: 3
Length of query: 591
Length of database: 11,343,932
Length adjustment: 105
Effective length of query: 486
Effective length of database: 8,729,747
Effective search space: 4242657042
Effective search space used: 4242657042
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012772
(1773 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_057918.2| methylglutaconyl-CoA hydratase, mitochondrial p... 276 3e-74
Alignment gi|NP_081004.2| enoyl-CoA hydratase domain-containing protein 2... 123 4e-28
Alignment gi|NP_444349.1| enoyl-CoA hydratase, mitochondrial precursor [M... 49 1e-05
>ref|NP_057918.2| methylglutaconyl-CoA hydratase, mitochondrial precursor [Mus
musculus].
Length = 314
Score = 276 bits (707), Expect = 3e-74
Identities = 153/215 (71%), Positives = 161/215 (74%)
Frame = +2
Query: 53 AAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGGAAWR 232
AA PGALG LR RLVAA CA +GP A WARG P R
Sbjct: 2 AAAAPGALGALRTVGVRLVAACCARLGP---------------AAWARGTAP-------R 39
Query: 233 RGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDKKV 412
RGYSSE KTEDELRVR+LEEENRGIVVLGINRAY KN+LSKNL+KMLSKAVDALKSDKKV
Sbjct: 40 RGYSSEVKTEDELRVRHLEEENRGIVVLGINRAYGKNALSKNLLKMLSKAVDALKSDKKV 99
Query: 413 RTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIANLPVPTIXXXXXXXX 592
RTII+RSEVPGIFCAGADLKER KMHSSEVGPFVSK+R+VIN+IANLPVPTI
Sbjct: 100 RTIIIRSEVPGIFCAGADLKERAKMHSSEVGPFVSKIRSVINDIANLPVPTIAAIDGLAL 159
Query: 593 XXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
CDIRVAASSAKMGLVETKLAIIPGGG
Sbjct: 160 GGGLELALACDIRVAASSAKMGLVETKLAIIPGGG 194
>ref|NP_081004.2| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
precursor [Mus musculus].
Length = 296
Score = 123 bits (309), Expect = 4e-28
Identities = 70/163 (42%), Positives = 98/163 (60%), Gaps = 5/163 (3%)
Frame = +2
Query: 224 AWR----RGYSSEAKTED-ELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVD 388
+WR R +S A T E++V+ L N+GI + +NR A+N+L V L +A+
Sbjct: 14 SWRFSGARDCASHATTRTPEIQVQALTGPNQGITEILMNRPNARNALGNVFVSELLEALA 73
Query: 389 ALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIANLPVPTI 568
L+ D++VR ++ RS V G+FCAGADLKER +M EVG FV +LR +++EIA PVPTI
Sbjct: 74 QLREDQQVRVLLFRSAVKGVFCAGADLKEREQMSDVEVGTFVQRLRGLMSEIAAFPVPTI 133
Query: 569 XXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
CD+R+AASSA MGL+ET ++PG G
Sbjct: 134 AAMDGFALGGGLELALACDLRIAASSAVMGLIETTRGLLPGAG 176
>ref|NP_444349.1| enoyl-CoA hydratase, mitochondrial precursor [Mus musculus].
Length = 290
Score = 49.3 bits (116), Expect = 1e-05
Identities = 36/135 (26%), Positives = 58/135 (42%)
Frame = +2
Query: 293 ENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLK 472
+N + ++ +NR A N+L L++ L++A++ + D V I++ F AGAD+K
Sbjct: 43 KNSSVGLIQLNRPKALNALCNGLIEELNQALETFEQDPAVGAIVLTGGDKA-FAAGADIK 101
Query: 473 ERVKMHSSEVGPFVSKLRAVINEIANLPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAK 652
E + + SK + + I + P I CDI A A+
Sbjct: 102 EMQNRTFQDC--YSSKFLSHWDHITRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQ 159
Query: 653 MGLVETKLAIIPGGG 697
G E L IPG G
Sbjct: 160 FGQPEILLGTIPGAG 174
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 38,051,637
Number of extensions: 907090
Number of successful extensions: 2860
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 2852
Number of HSP's successfully gapped: 3
Length of query: 591
Length of database: 15,617,559
Length adjustment: 107
Effective length of query: 484
Effective length of database: 12,403,707
Effective search space: 6003394188
Effective search space used: 6003394188
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012772
(1773 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001689.1| methylglutaconyl-CoA hydratase, mitochondrial p... 320 3e-87
Alignment gi|NP_001185890.1| enoyl-CoA hydratase domain-containing protei... 122 1e-27
Alignment gi|NP_001185891.1| enoyl-CoA hydratase domain-containing protei... 92 2e-18
Alignment gi|NP_060751.2| enoyl-CoA hydratase domain-containing protein 2... 92 2e-18
>ref|NP_001689.1| methylglutaconyl-CoA hydratase, mitochondrial precursor [Homo
sapiens].
Length = 339
Score = 320 bits (820), Expect = 3e-87
Identities = 169/217 (77%), Positives = 178/217 (82%)
Frame = +2
Query: 47 AAAAGVPGALGTLRAGRARLVAAYCAGIGPGWRLPGSWTGLPVGPAIWARGWTPAAGGAA 226
AA A PGALG+L AG ARLVAA A + PG RLPGS G GPAIWA+GW PAAGG A
Sbjct: 3 AAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPA 62
Query: 227 WRRGYSSEAKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKMLSKAVDALKSDK 406
+RGYSSE KTEDELRVR+LEEENRGIVVLGINRAY KNSLSKNL+KMLSKAVDALKSDK
Sbjct: 63 PKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDK 122
Query: 407 KVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIANLPVPTIXXXXXX 586
KVRTII+RSEVPGIFCAGADLKER KM SSEVGPFVSK+RAVIN+IANLPVPTI
Sbjct: 123 KVRTIIIRSEVPGIFCAGADLKERAKMSSSEVGPFVSKIRAVINDIANLPVPTIAAIDGL 182
Query: 587 XXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
CDIRVAASSAKMGLVETKLAIIPGGG
Sbjct: 183 ALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGG 219
>ref|NP_001185890.1| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
isoform 1 [Homo sapiens].
Length = 292
Score = 122 bits (306), Expect = 1e-27
Identities = 70/169 (41%), Positives = 98/169 (57%), Gaps = 1/169 (0%)
Frame = +2
Query: 194 RGWTPAAGGAAWRRGYSSE-AKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKM 370
R W P RG +S+ A E++VR L ++GI + +NR A+N+L V
Sbjct: 9 RPWRPLRA-----RGCASDGAAGGSEIQVRALAGPDQGITEILMNRPSARNALGNVFVSE 63
Query: 371 LSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIAN 550
L + + L+ D++VR ++ RS V G+FCAGADLKER +M +EVG FV +LR ++N+IA
Sbjct: 64 LLETLAQLREDRQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGVFVQRLRGLMNDIAA 123
Query: 551 LPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
P PTI CD+RVAASSA MGL+ET ++PG G
Sbjct: 124 FPAPTIAAMDGFALGGGLELALACDLRVAASSAVMGLIETTRGLLPGAG 172
>ref|NP_001185891.1| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
isoform 3 [Homo sapiens].
Length = 244
Score = 91.7 bits (226), Expect = 2e-18
Identities = 60/169 (35%), Positives = 87/169 (51%), Gaps = 1/169 (0%)
Frame = +2
Query: 194 RGWTPAAGGAAWRRGYSSE-AKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKM 370
R W P RG +S+ A E++VR L ++GI + +NR A+N+L V
Sbjct: 9 RPWRPLRA-----RGCASDGAAGGSEIQVRALAGPDQGITEILMNRPSARNALGNVFVSE 63
Query: 371 LSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIAN 550
L + + L+ D++VR ++ RS V G+FCAGADLKER +M +EVG FV +LR ++N+I
Sbjct: 64 LLETLAQLREDRQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGVFVQRLRGLMNDI-- 121
Query: 551 LPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
ASSA MGL+ET ++PG G
Sbjct: 122 -----------------------------ASSAVMGLIETTRGLLPGAG 141
>ref|NP_060751.2| enoyl-CoA hydratase domain-containing protein 2, mitochondrial
isoform 2 [Homo sapiens].
Length = 261
Score = 91.7 bits (226), Expect = 2e-18
Identities = 60/169 (35%), Positives = 87/169 (51%), Gaps = 1/169 (0%)
Frame = +2
Query: 194 RGWTPAAGGAAWRRGYSSE-AKTEDELRVRYLEEENRGIVVLGINRAYAKNSLSKNLVKM 370
R W P RG +S+ A E++VR L ++GI + +NR A+N+L V
Sbjct: 9 RPWRPLRA-----RGCASDGAAGGSEIQVRALAGPDQGITEILMNRPSARNALGNVFVSE 63
Query: 371 LSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMHSSEVGPFVSKLRAVINEIAN 550
L + + L+ D++VR ++ RS V G+FCAGADLKER +M +EVG FV +LR ++N+I
Sbjct: 64 LLETLAQLREDRQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGVFVQRLRGLMNDI-- 121
Query: 551 LPVPTIXXXXXXXXXXXXXXXXXCDIRVAASSAKMGLVETKLAIIPGGG 697
ASSA MGL+ET ++PG G
Sbjct: 122 -----------------------------ASSAVMGLIETTRGLLPGAG 141
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 44,332,430
Number of extensions: 1033530
Number of successful extensions: 3561
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 3542
Number of HSP's successfully gapped: 6
Length of query: 591
Length of database: 18,297,164
Length adjustment: 108
Effective length of query: 483
Effective length of database: 14,737,052
Effective search space: 7117996116
Effective search space used: 7117996116
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012772
(1773 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_Chr14 1070 0.0
>Sscrofa_Chr14
|| Length = 153851969
Score = 1070 bits (540), Expect = 0.0
Identities = 549/552 (99%)
Strand = Plus / Minus
Query: 637 agcttcctccgccaaaatgggcctggttgaaacaaagttggcaattattcctggtggagg 696
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3160153 agcttcctccgccaaaatgggcctggttgaaacaaagttggcaattattcctggtggagg 3160094
Query: 697 tatgaattgttcatgcccatcttgatttttgttcctcaagatagagcataaggcaaaaag 756
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3160093 tatgaattgttcatgcccatcttgatttttgttcctcaagatagagcataaggcaaaaag 3160034
Query: 757 gtgttagctgaaaaggcaacatttgaggtaaagagactaatgctgtattgtttgcttctg 816
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3160033 gtgttagctgaaaaggcaacatttgaggtaaagagactaatgctgtattgtttgcttctg 3159974
Query: 817 tagcaaagtccttattcaaaatgcattcttttagcttctgcacatgtgaagatcgcaggt 876
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3159973 tagcaaagtccttattcaaaatgcattcttttagcttctgcacatgtgaagatcgcaggt 3159914
Query: 877 attgcctgttgctctctttttgtgctatgtcctgggcttttctgtatggtttcttaacta 936
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3159913 attgcctgttgctctctttttgtgctatgtcctgggcttttctgtatggtttcttaacta 3159854
Query: 937 aattattgtaccagtcacattactcttcaagaattgcactgatatttttacacttttgtg 996
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct: 3159853 aattattgtaccagtcacattactcttcaagaattgcacttatatttttacacttttgtg 3159794
Query: 997 tggatgtgtgcaccctcagagagctttatgtacacacttagaccccagtggaggaagggc 1056
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 3159793 tggatgtgtgcaccctcagagagctttatgtacacacttagaccccagtcgaggaagggc 3159734
Query: 1057 agtgcttccctggcttcgcgccagcttacttgtgtttctgtgcagctggacaaaagttgg 1116
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3159733 agtgcttccctggcttcgcgccagcttacttgtgtttctgtgcagctggacaaaagttgg 3159674
Query: 1117 tgttagtgggtagtttgaatttggatacttctgtacaagtgatacttctgtcactatgag 1176
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3159673 tgttagtgggtagtctgaatttggatacttctgtacaagtgatacttctgtcactatgag 3159614
Query: 1177 aaagatagatga 1188
||||||||||||
Sbjct: 3159613 aaagatagatga 3159602
Score = 601 bits (303), Expect = e-169
Identities = 303/303 (100%)
Strand = Plus / Minus
Query: 1 tgcggcgtgcagcgaccgcaggcccacgccgtaaacagagaacatggcggcggcagcggg 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3222307 tgcggcgtgcagcgaccgcaggcccacgccgtaaacagagaacatggcggcggcagcggg 3222248
Query: 61 ggtacctggggccttaggtaccctgcgggcaggccgcgcccgcttggtggctgcttactg 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3222247 ggtacctggggccttaggtaccctgcgggcaggccgcgcccgcttggtggctgcttactg 3222188
Query: 121 tgcggggatcggccccgggtggaggctccctggctcctggaccggcctgccggtagggcc 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3222187 tgcggggatcggccccgggtggaggctccctggctcctggaccggcctgccggtagggcc 3222128
Query: 181 ggccatctgggctcggggctggacgcccgcggccgggggtgcggcctggcgaaggggcta 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3222127 ggccatctgggctcggggctggacgcccgcggccgggggtgcggcctggcgaaggggcta 3222068
Query: 241 cagctctgaggcgaagacggaggacgagctgcgggtgcgatatctggaggaggagaaccg 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3222067 cagctctgaggcgaagacggaggacgagctgcgggtgcgatatctggaggaggagaaccg 3222008
Query: 301 agg 303
|||
Sbjct: 3222007 agg 3222005
Score = 186 bits (94), Expect = 3e-44
Identities = 94/94 (100%)
Strand = Plus / Minus
Query: 545 gctaatcttccagtgccaaccattgcagcaatagatggactcgctttaggtggtggactt 604
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3161912 gctaatcttccagtgccaaccattgcagcaatagatggactcgctttaggtggtggactt 3161853
Query: 605 gaactggctttagcatgtgatataagagtagcag 638
||||||||||||||||||||||||||||||||||
Sbjct: 3161852 gaactggctttagcatgtgatataagagtagcag 3161819
Score = 180 bits (91), Expect = 2e-42
Identities = 91/91 (100%)
Strand = Plus / Minus
Query: 370 gctgtcaaaagctgtggatgctttaaaatctgataagaaagtacggaccataatagtcag 429
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3205662 gctgtcaaaagctgtggatgctttaaaatctgataagaaagtacggaccataatagtcag 3205603
Query: 430 aagtgaagtcccagggatattctgtgctggt 460
|||||||||||||||||||||||||||||||
Sbjct: 3205602 aagtgaagtcccagggatattctgtgctggt 3205572
Score = 174 bits (88), Expect = 1e-40
Identities = 88/88 (100%)
Strand = Plus / Minus
Query: 458 ggtgctgaccttaaggaaagagtcaaaatgcattccagtgaagttggtccttttgtctcc 517
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3194731 ggtgctgaccttaaggaaagagtcaaaatgcattccagtgaagttggtccttttgtctcc 3194672
Query: 518 aaattaagagcagtgattaatgaaatcg 545
||||||||||||||||||||||||||||
Sbjct: 3194671 aaattaagagcagtgattaatgaaatcg 3194644
Score = 139 bits (70), Expect = 6e-30
Identities = 70/70 (100%)
Strand = Plus / Minus
Query: 301 aggaattgtggtgctcggaataaacagagcttatgccaaaaattcactgagtaaaaatct 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3205832 aggaattgtggtgctcggaataaacagagcttatgccaaaaattcactgagtaaaaatct 3205773
Query: 361 tgtaaaaatg 370
||||||||||
Sbjct: 3205772 tgtaaaaatg 3205763
Score = 103 bits (52), Expect = 3e-19
Identities = 52/52 (100%)
Strand = Plus / Minus
Query: 1442 taggtgagctgtgtcttgtggaagactcagttaagaaagcccttttcttaat 1493
||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3159348 taggtgagctgtgtcttgtggaagactcagttaagaaagcccttttcttaat 3159297
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 38,025,392
Number of extensions: 416
Number of successful extensions: 416
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 416
Number of HSP's successfully gapped: 7
Length of query: 1773
Length of database: 2,808,509,378
Length adjustment: 22
Effective length of query: 1751
Effective length of database: 2,808,408,574
Effective search space: 4917523413074
Effective search space used: 4917523413074
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)