Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012977
(1200 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_851024.1| PREDICTED: similar to 3-beta-hydroxysteroid-del... 450 e-126
Alignment gi|XP_853729.1| PREDICTED: similar to 3-beta-hydroxysteroid-del... 430 e-120
Alignment gi|XP_533979.2| PREDICTED: similar to cysteine and histidine-ri... 297 2e-80
Alignment gi|XP_862935.1| PREDICTED: similar to 3-beta-hydroxysteroid-del... 204 2e-52
Alignment gi|XP_534113.2| PREDICTED: similar to emopamil binding related ... 89 8e-18
>ref|XP_851024.1| PREDICTED: similar to
3-beta-hydroxysteroid-delta(8),delta(7)-isomerase
(Cholestenol delta-isomerase) (Delta8-delta7 sterol
isomerase) (D8-D7 sterol isomerase) (Emopamil-binding
protein) isoform 1 [Canis familiaris].
Length = 228
Score = 450 bits (1158), Expect = e-126
Identities = 203/227 (89%), Positives = 220/227 (96%)
Frame = +1
Query: 397 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 576
MTTNASP+HPYWPRHLRLDNFVPNDCPTWH+LAGLFS SGVLVVTTWLLSGRAAV+PL T
Sbjct: 1 MTTNASPLHPYWPRHLRLDNFVPNDCPTWHLLAGLFSVSGVLVVTTWLLSGRAAVIPLGT 60
Query: 577 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 756
WRRLSLCWFAVCGF+H+VIEGWF LYHE+LLGDQAFLSQLWKEYAKGDSRYIL+DNFTIC
Sbjct: 61 WRRLSLCWFAVCGFIHMVIEGWFSLYHEDLLGDQAFLSQLWKEYAKGDSRYILSDNFTIC 120
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 936
METITACLWGPLSLWVVIAFLRQQP RF+LQLVVSVGQIYGDVLYFLTEHRDGFQHGE+G
Sbjct: 121 METITACLWGPLSLWVVIAFLRQQPLRFILQLVVSVGQIYGDVLYFLTEHRDGFQHGEVG 180
Query: 937 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRARS 1077
HP+YFWFYFVFMN LWLVLPGILVLDS+KQL+HAQ +LD+K ++A+S
Sbjct: 181 HPLYFWFYFVFMNGLWLVLPGILVLDSVKQLAHAQSVLDAKGTKAKS 227
>ref|XP_853729.1| PREDICTED: similar to
3-beta-hydroxysteroid-delta(8),delta(7)-isomerase
(Cholestenol delta-isomerase) (Delta8-delta7 sterol
isomerase) (D8-D7 sterol isomerase) (Emopamil-binding
protein) [Canis familiaris].
Length = 228
Score = 430 bits (1105), Expect = e-120
Identities = 196/227 (86%), Positives = 215/227 (94%)
Frame = +1
Query: 397 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 576
MTTNASP+HPYWPR LRLDNFVPNDCPTWH+LAGLFS SGVLVVTTWLLSGRAAV+ L T
Sbjct: 1 MTTNASPLHPYWPRQLRLDNFVPNDCPTWHLLAGLFSVSGVLVVTTWLLSGRAAVILLGT 60
Query: 577 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 756
W+RLSLCWFAVCGF+H+VIEGWF LYH++LLGDQAFLSQLWKEYAKGDSRYIL+DNFTIC
Sbjct: 61 WQRLSLCWFAVCGFIHMVIEGWFSLYHKDLLGDQAFLSQLWKEYAKGDSRYILSDNFTIC 120
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 936
ETITACLW PLSLWVVIAFL QQP RFVLQL+VSVGQIYGDVLYFLTEHRDGFQHGELG
Sbjct: 121 TETITACLWRPLSLWVVIAFLLQQPLRFVLQLMVSVGQIYGDVLYFLTEHRDGFQHGELG 180
Query: 937 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRARS 1077
HP+YFWFY VFMN+LWLVLPGILVLDS+KQL+HAQ +LD+K ++A+S
Sbjct: 181 HPLYFWFYCVFMNSLWLVLPGILVLDSVKQLAHAQSVLDAKGTKAKS 227
>ref|XP_533979.2| PREDICTED: similar to cysteine and histidine-rich domain
(CHORD)-containing, zinc-binding protein 1 [Canis
familiaris].
Length = 642
Score = 297 bits (760), Expect = 2e-80
Identities = 135/155 (87%), Positives = 142/155 (91%)
Frame = +1
Query: 397 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 576
MTTNAS HPYWPRHLRLDNFVPNDCPTWH+LA LFS SGVLV TTWLLSG A V+PL T
Sbjct: 1 MTTNASSSHPYWPRHLRLDNFVPNDCPTWHLLASLFSVSGVLVATTWLLSGSAVVMPLGT 60
Query: 577 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 756
WRRLSLCWFAVCGF+H+VIEGWF LYHE+LLGDQAFLSQLWKEYAKGDSRYIL+DNFTIC
Sbjct: 61 WRRLSLCWFAVCGFIHMVIEGWFSLYHEDLLGDQAFLSQLWKEYAKGDSRYILSDNFTIC 120
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVS 861
ME ITACLWGPLS WVVIAFLRQQP F+LQLVVS
Sbjct: 121 MEAITACLWGPLSRWVVIAFLRQQPLSFLLQLVVS 155
>ref|XP_862935.1| PREDICTED: similar to
3-beta-hydroxysteroid-delta(8),delta(7)-isomerase
(Cholestenol delta-isomerase) (Delta8-delta7 sterol
isomerase) (D8-D7 sterol isomerase) (Emopamil-binding
protein) isoform 2 [Canis familiaris].
Length = 108
Score = 204 bits (518), Expect = 2e-52
Identities = 93/107 (86%), Positives = 103/107 (96%)
Frame = +1
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 936
METITACLWGPLSLWVVIAFLRQQP RF+LQLVVSVGQIYGDVLYFLTEHRDGFQHGE+G
Sbjct: 1 METITACLWGPLSLWVVIAFLRQQPLRFILQLVVSVGQIYGDVLYFLTEHRDGFQHGEVG 60
Query: 937 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRARS 1077
HP+YFWFYFVFMN LWLVLPGILVLDS+KQL+HAQ +LD+K ++A+S
Sbjct: 61 HPLYFWFYFVFMNGLWLVLPGILVLDSVKQLAHAQSVLDAKGTKAKS 107
>ref|XP_534113.2| PREDICTED: similar to emopamil binding related protein, delta8-delta7
sterol isomerase [Canis familiaris].
Length = 198
Score = 89.0 bits (219), Expect = 8e-18
Identities = 52/177 (29%), Positives = 93/177 (52%), Gaps = 6/177 (3%)
Frame = +1
Query: 517 VLVVTTWLLSGRAAVVPLSTWR----RLSLCWFAVCGFVHLVIEGWFCL--YHEELLGDQ 678
+L+ + LL+G A + L R R L W VHL +EG F + + +
Sbjct: 7 LLLCASLLLAGCALGLRLGRGRGAADRAVLAWLCYDALVHLALEGPFVYLSFAGSVADSE 66
Query: 679 AFLSQLWKEYAKGDSRYILNDNFTICMETITACLWGPLSLWVVIAFLRQQPHRFVLQLVV 858
++ LWKEY K D+R++ D + +E +T L G L+L +V A + ++ +R +Q+ +
Sbjct: 67 GLIASLWKEYGKADARWLYFDPTIVSLEVLTVVLDGSLALVLVYAIVTEKYYRHFIQVTL 126
Query: 859 SVGQIYGDVLYFLTEHRDGFQHGELGHPIYFWFYFVFMNALWLVLPGILVLDSIKQL 1029
V ++YG + F+ + G + +YF Y VF N++W+++PG+L+ S +L
Sbjct: 127 CVCELYGGWMTFVPDWLVGSPNLNTDDWLYFGVYLVFFNSVWVLIPGLLLCQSWVEL 183
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 46,104,459
Number of extensions: 1212446
Number of successful extensions: 4430
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 4424
Number of HSP's successfully gapped: 5
Length of query: 400
Length of database: 18,874,504
Length adjustment: 105
Effective length of query: 295
Effective length of database: 15,374,224
Effective search space: 4535396080
Effective search space used: 4535396080
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Animal-Genome cDNA 20110601C-012977
Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012977
(1200 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001029672.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isom... 430 e-120
Alignment gi|NP_001192668.1| emopamil-binding protein-like [Bos taurus]. 89 6e-18
Alignment gi|XP_002691837.1| PREDICTED: emopamil binding related protein,... 89 6e-18
>ref|NP_001029672.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Bos taurus].
Length = 230
Score = 430 bits (1106), Expect = e-120
Identities = 196/229 (85%), Positives = 214/229 (93%)
Frame = +1
Query: 397 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 576
MTTN SPMHPYWPRHLRLDNFVPND PTWHILAGLFS SGVLVV TWLLSGRAAVVPL T
Sbjct: 1 MTTNTSPMHPYWPRHLRLDNFVPNDYPTWHILAGLFSVSGVLVVATWLLSGRAAVVPLGT 60
Query: 577 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 756
WRRLSLCWFAVCGF+HLVIEGWF LYH +LLGDQA LSQLWKEYAKGDSRYILNDNF IC
Sbjct: 61 WRRLSLCWFAVCGFIHLVIEGWFSLYHADLLGDQAILSQLWKEYAKGDSRYILNDNFMIC 120
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 936
MET+TA LWGPLSLWVVIAFLR QP RFVLQLVVS+GQ+YGDVLYFLTE+RDGFQHGELG
Sbjct: 121 METVTAYLWGPLSLWVVIAFLRHQPLRFVLQLVVSMGQVYGDVLYFLTEYRDGFQHGELG 180
Query: 937 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRARSSK 1083
HP+YFWFYFVF+N+LWLV+PG+L+LDSIKQL+HAQ +LD+KA +A+S +
Sbjct: 181 HPLYFWFYFVFLNSLWLVVPGLLILDSIKQLAHAQSILDAKAPKAKSKQ 229
>ref|NP_001192668.1| emopamil-binding protein-like [Bos taurus].
Length = 206
Score = 89.4 bits (220), Expect = 6e-18
Identities = 53/189 (28%), Positives = 94/189 (49%), Gaps = 9/189 (4%)
Frame = +1
Query: 496 GLFSASGVLVVTTWLLSGRAAVVPLSTWR----RLSLCWFAVCGFVHLVIEGWFCLYHE- 660
G + +L+ + L G A + L R R L W VH +EG +
Sbjct: 8 GAAAGGSLLLCASLLAVGCALGLRLGRGRGAADRGLLAWLCFNALVHFALEGPYVYLSMV 67
Query: 661 -ELLGDQAFLSQLWKEYAKGDSRYILNDNFTICMETITACLWGPLSLWVVIAFLRQQPHR 837
+ A ++ LWKEY K D+R++ D + +E +T L G L+L +V A ++++ +R
Sbjct: 68 GNITHSDALIASLWKEYGKADARWLYFDPTIVSVEILTVVLGGSLALVLVYAIVKEKHYR 127
Query: 838 FVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELGHPIYFWFYFVFMNALWLVLPGILVLDS 1017
+Q+ + V ++YG + F + G + +YFW Y VF N +W+++PG+L+ S
Sbjct: 128 HFVQITLCVCELYGGWMTFCPDWLMGSPNLNTNSWLYFWVYLVFFNGVWVLIPGLLLWQS 187
Query: 1018 ---IKQLSH 1035
+K++ H
Sbjct: 188 WVELKKMHH 196
>ref|XP_002691837.1| PREDICTED: emopamil binding related protein, delta8-delta7 sterol
isomerase-like [Bos taurus].
Length = 206
Score = 89.4 bits (220), Expect = 6e-18
Identities = 53/189 (28%), Positives = 94/189 (49%), Gaps = 9/189 (4%)
Frame = +1
Query: 496 GLFSASGVLVVTTWLLSGRAAVVPLSTWR----RLSLCWFAVCGFVHLVIEGWFCLYHE- 660
G + +L+ + L G A + L R R L W VH +EG +
Sbjct: 8 GAAAGGSLLLCASLLAVGCALGLRLGRGRGAADRGLLAWLCFNALVHFALEGPYVYLSMV 67
Query: 661 -ELLGDQAFLSQLWKEYAKGDSRYILNDNFTICMETITACLWGPLSLWVVIAFLRQQPHR 837
+ A ++ LWKEY K D+R++ D + +E +T L G L+L +V A ++++ +R
Sbjct: 68 GNITHSDALIASLWKEYGKADARWLYFDPTIVSVEILTVVLGGSLALVLVYAIVKEKHYR 127
Query: 838 FVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELGHPIYFWFYFVFMNALWLVLPGILVLDS 1017
+Q+ + V ++YG + F + G + +YFW Y VF N +W+++PG+L+ S
Sbjct: 128 HFVQITLCVCELYGGWMTFCPDWLMGSPNLNTNSWLYFWVYLVFFNGVWVLIPGLLLWQS 187
Query: 1018 ---IKQLSH 1035
+K++ H
Sbjct: 188 WVELKKMHH 196
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 44,141,459
Number of extensions: 1174654
Number of successful extensions: 4420
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 4408
Number of HSP's successfully gapped: 3
Length of query: 400
Length of database: 17,681,374
Length adjustment: 104
Effective length of query: 296
Effective length of database: 14,240,222
Effective search space: 4215105712
Effective search space used: 4215105712
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012977
(1200 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001161118.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isom... 483 e-136
Alignment gi|XP_003131007.1| PREDICTED: emopamil-binding protein-like iso... 95 9e-20
>ref|NP_001161118.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Sus scrofa].
Length = 230
Score = 483 bits (1242), Expect = e-136
Identities = 226/229 (98%), Positives = 228/229 (99%)
Frame = +1
Query: 397 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 576
MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST
Sbjct: 1 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 60
Query: 577 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 756
WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC
Sbjct: 61 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 120
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 936
METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG
Sbjct: 121 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 180
Query: 937 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRARSSK 1083
HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRA+S +
Sbjct: 181 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRAKSKQ 229
>ref|XP_003131007.1| PREDICTED: emopamil-binding protein-like isoform 1 [Sus scrofa].
Length = 206
Score = 94.7 bits (234), Expect = 9e-20
Identities = 58/195 (29%), Positives = 105/195 (53%), Gaps = 9/195 (4%)
Frame = +1
Query: 472 CPTWHILAGLFSASGVLVVTTWLLSGRAAV-VPLSTWR----RLSLCWFAVCGFVHLVIE 636
C W + + +A G L++T LL+ A+ + L R R +L W VH +E
Sbjct: 2 CAGWELGS---AAGGSLLLTAALLAVSCALGLRLGRGRGAADRGALAWLCFDALVHFALE 58
Query: 637 GWFCLYHEELLGD----QAFLSQLWKEYAKGDSRYILNDNFTICMETITACLWGPLSLWV 804
G F + L+G+ A ++ LWKEY K D+R++ +D + +E +T L G L+L +
Sbjct: 59 GPFV--YLSLVGNITDSDALIASLWKEYGKADARWLTSDPTIVALEILTVVLDGSLALVL 116
Query: 805 VIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELGHPIYFWFYFVFMNALW 984
+ A ++++ +R +Q+ + V ++YG + F + G + + +YFW Y VF N +W
Sbjct: 117 IYAIVKEKYYRHFVQITLCVCELYGGWMTFCPDWLLGSPNLSTNNWLYFWVYLVFFNGVW 176
Query: 985 LVLPGILVLDSIKQL 1029
+++PG+L+ S +L
Sbjct: 177 VLIPGLLLWQSWVEL 191
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 28,469,657
Number of extensions: 754964
Number of successful extensions: 2814
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 2813
Number of HSP's successfully gapped: 2
Length of query: 400
Length of database: 11,343,932
Length adjustment: 101
Effective length of query: 299
Effective length of database: 8,829,335
Effective search space: 2639971165
Effective search space used: 2639971165
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012977
(1200 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_031924.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomera... 379 e-105
Alignment gi|NP_080874.2| emopamil-binding protein-like [Mus musculus]. 95 1e-19
>ref|NP_031924.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Mus musculus].
Length = 230
Score = 379 bits (973), Expect = e-105
Identities = 174/227 (76%), Positives = 198/227 (87%)
Frame = +1
Query: 397 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 576
MTTN P+HPYWPRHL+LDNFVPND PT HIL GLFS SG L+V TWLLS RA+VVPL
Sbjct: 1 MTTNTVPLHPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLIVITWLLSSRASVVPLGA 60
Query: 577 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 756
RRL+LCWFAVC F+HLVIEGWF LY+ LL DQAFLSQLWKEY+KGDSRYIL+D+F +C
Sbjct: 61 GRRLALCWFAVCTFIHLVIEGWFSLYNGILLEDQAFLSQLWKEYSKGDSRYILSDSFVVC 120
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 936
MET+TACLWGPLSLWVVIAFLRQQP RFVLQLVVS+GQIYGDVLYFLTE +G QHGE+G
Sbjct: 121 METVTACLWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIG 180
Query: 937 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRARS 1077
HP+YFWFYFVF+NA+WLV+P ILVLD+IK L+ AQ +LDSK + +S
Sbjct: 181 HPVYFWFYFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMKIKS 227
>ref|NP_080874.2| emopamil-binding protein-like [Mus musculus].
Length = 206
Score = 94.7 bits (234), Expect = 1e-19
Identities = 57/188 (30%), Positives = 94/188 (50%), Gaps = 6/188 (3%)
Frame = +1
Query: 484 HILAGLFSASGVLVVTTWLLSGRAAVVPLSTWR----RLSLCWFAVCGFVHLVIEGWFCL 651
H G + S +L+ + L G A + L R R L W VH V+EG F
Sbjct: 4 HWALGPEAGSSLLLCSALLAVGCALGLRLGRGRSAVERWVLAWLCYDSLVHFVLEGAFVY 63
Query: 652 YH--EELLGDQAFLSQLWKEYAKGDSRYILNDNFTICMETITACLWGPLSLWVVIAFLRQ 825
+ Q ++ LWKEY K D+R++ +D + +E +T L G L+L ++ A +++
Sbjct: 64 LSIVGNVADSQGLIASLWKEYGKADTRWLYSDPTVVSLEILTVVLDGLLALVLIYAIVKE 123
Query: 826 QPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELGHPIYFWFYFVFMNALWLVLPGIL 1005
+ +R +Q+V+ V ++YG + F E G +Y W Y VF N LW+++PG+L
Sbjct: 124 KYYRHFVQIVLCVCELYGCWMTFFPEWLVGSPSLNTSSWLYLWVYLVFFNGLWVLIPGLL 183
Query: 1006 VLDSIKQL 1029
+ S +L
Sbjct: 184 LWQSWVEL 191
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 38,359,180
Number of extensions: 1004625
Number of successful extensions: 3574
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 3564
Number of HSP's successfully gapped: 2
Length of query: 400
Length of database: 15,617,559
Length adjustment: 104
Effective length of query: 296
Effective length of database: 12,493,815
Effective search space: 3698169240
Effective search space used: 3698169240
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012977
(1200 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_006570.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomera... 440 e-123
Alignment gi|NP_115954.1| emopamil-binding protein-like [Homo sapiens]. 88 2e-17
>ref|NP_006570.1| 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Homo sapiens].
Length = 230
Score = 440 bits (1132), Expect = e-123
Identities = 200/229 (87%), Positives = 217/229 (94%)
Frame = +1
Query: 397 MTTNASPMHPYWPRHLRLDNFVPNDCPTWHILAGLFSASGVLVVTTWLLSGRAAVVPLST 576
MTTNA P+HPYWP+HLRLDNFVPND PTWHILAGLFS +GVLVVTTWLLSGRAAVVPL T
Sbjct: 1 MTTNAGPLHPYWPQHLRLDNFVPNDRPTWHILAGLFSVTGVLVVTTWLLSGRAAVVPLGT 60
Query: 577 WRRLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQAFLSQLWKEYAKGDSRYILNDNFTIC 756
WRRLSLCWFAVCGF+HLVIEGWF LY+E+LLGDQAFLSQLWKEYAKGDSRYIL DNFT+C
Sbjct: 61 WRRLSLCWFAVCGFIHLVIEGWFVLYYEDLLGDQAFLSQLWKEYAKGDSRYILGDNFTVC 120
Query: 757 METITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 936
METITACLWGPLSLWVVIAFLRQ P RF+LQLVVSVGQIYGDVLYFLTEHRDGFQHGELG
Sbjct: 121 METITACLWGPLSLWVVIAFLRQHPLRFILQLVVSVGQIYGDVLYFLTEHRDGFQHGELG 180
Query: 937 HPIYFWFYFVFMNALWLVLPGILVLDSIKQLSHAQRMLDSKASRARSSK 1083
HP+YFWFYFVFMNALWLVLPG+LVLD++K L+HAQ LD+KA++A+S K
Sbjct: 181 HPLYFWFYFVFMNALWLVLPGVLVLDAVKHLTHAQSTLDAKATKAKSKK 229
>ref|NP_115954.1| emopamil-binding protein-like [Homo sapiens].
Length = 206
Score = 87.8 bits (216), Expect = 2e-17
Identities = 47/153 (30%), Positives = 84/153 (54%), Gaps = 4/153 (2%)
Frame = +1
Query: 583 RLSLCWFAVCGFVHLVIEGWFCLYHEELLGDQA----FLSQLWKEYAKGDSRYILNDNFT 750
R +L W VH +EG F + L+G+ A ++ LWKEY K D+R++ D
Sbjct: 41 RGALIWLCYDALVHFALEGPFV--YLSLVGNVANSDGLIASLWKEYGKADARWVYFDPTI 98
Query: 751 ICMETITACLWGPLSLWVVIAFLRQQPHRFVLQLVVSVGQIYGDVLYFLTEHRDGFQHGE 930
+ +E +T L G L+L+++ A ++++ +R LQ+ + V ++YG + FL E +
Sbjct: 99 VSVEILTVALDGSLALFLIYAIVKEKYYRHFLQITLCVCELYGCWMTFLPEWLTRSPNLN 158
Query: 931 LGHPIYFWFYFVFMNALWLVLPGILVLDSIKQL 1029
+ +Y W Y F N +W+++PG+L+ S +L
Sbjct: 159 TSNWLYCWLYLFFFNGVWVLIPGLLLWQSWLEL 191
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 45,205,926
Number of extensions: 1191161
Number of successful extensions: 4292
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 4285
Number of HSP's successfully gapped: 2
Length of query: 400
Length of database: 18,297,164
Length adjustment: 105
Effective length of query: 295
Effective length of database: 14,835,944
Effective search space: 4376603480
Effective search space used: 4376603480
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-012977
(1200 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_ChrX 724 0.0
>Sscrofa_ChrX
|| Length = 144288218
Score = 724 bits (365), Expect = 0.0
Identities = 371/373 (99%)
Strand = Plus / Minus
Query: 326 ggtttttcctctttctttgttctcctttctacatatccatacacagcccgcagctgtcag 385
||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 47626069 ggtttttcctctttctttgttctcctttctacatatccatacacagcccacagctgtcag 47626010
Query: 386 ccgacaaagacatgaccaccaacgccagccccatgcatccatactggcctcggcacctga 445
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47626009 ccgacaaagacatgaccaccaacgccagccccatgcatccatactggcctcggcacctga 47625950
Query: 446 ggctggacaactttgtgcctaatgactgccccacttggcatatcctggctggcctcttct 505
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47625949 ggctggacaactttgtgcctaatgactgccccacttggcatatcctggctggcctcttct 47625890
Query: 506 ccgcctctggagtcttagtcgtgactacctggctgttgtcaggtcgtgctgcggtcgtcc 565
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
Sbjct: 47625889 ccgcctctggagtcttagtcgtcactacctggctgttgtcaggtcgtgctgcggtcgtcc 47625830
Query: 566 cactgagcacttggcggagactgtccttgtgctggtttgcagtctgtgggttcgttcact 625
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47625829 cactgagcacttggcggagactgtccttgtgctggtttgcagtctgtgggttcgttcact 47625770
Query: 626 tggtgattgagggctggttctgtctctaccacgaggagcttcttggagaccaagccttct 685
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47625769 tggtgattgagggctggttctgtctctaccacgaggagcttcttggagaccaagccttct 47625710
Query: 686 tatctcaactctg 698
|||||||||||||
Sbjct: 47625709 tatctcaactctg 47625697
Score = 634 bits (320), Expect = e-179
Identities = 326/328 (99%)
Strand = Plus / Minus
Query: 1 tgaccgaccctcggcgcgcctgcgcgagaccctttctctccgccacccgcctgccaaccc 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47627241 tgaccgaccctcggcgcgcctgcgcgagaccctttctctccgccacccgcctgccaaccc 47627182
Query: 61 acgccgtgcctccccgccctcccacgcgcttgcgcaggatccccggctaccgcgctgttg 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47627181 acgccgtgcctccccgccctcccacgcgcttgcgcaggatccccggctaccgcgctgttg 47627122
Query: 121 tccggacgacagaagatccaccttctcattgggcagctggagtagaggattctggttttg 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47627121 tccggacgacagaagatccaccttctcattgggcagctggagtagaggattctggttttg 47627062
Query: 181 attggtgatctctgtctttgaccctccttttcctttttagcgccccaagctgggatcctg 240
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47627061 attggtggtctctgtctttgaccctccttttcctttttagcgccccaagctgggatcctg 47627002
Query: 241 tcctccacgttcctcttccttgcaggttggctgcgggttgcactctttcaggagggatcg 300
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47627001 tcctccacgttcctctttcttgcaggttggctgcgggttgcactctttcaggagggatcg 47626942
Query: 301 ggcaatcccacacactgaacctgagggt 328
||||||||||||||||||||||||||||
Sbjct: 47626941 ggcaatcccacacactgaacctgagggt 47626914
Score = 543 bits (274), Expect = e-152
Identities = 324/338 (95%), Gaps = 2/338 (0%)
Strand = Plus / Minus
Query: 865 ggtcagatatacggggatgtgctctatttcctgacagagcaccgtgacggattccagcac 924
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47619949 ggtcagatatacggggatgtgctctatttcctgacagagcaccgtgacggattccagcac 47619890
Query: 925 ggggagctgggccaccccatctacttctggttttactttgtcttcatgaatgctctgtgg 984
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47619889 ggggagctgggccacccaatctacttctggttttactttgtcttcatgaatgctctgtgg 47619830
Query: 985 ctggtgctgcccggaatccttgtgcttgattcgataaagcaactcagtcacgcccagagg 1044
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47619829 ctggtgctgcccggaatccttgtgcttgattcgataaagcaactcagtcacgcccagagg 47619770
Query: 1045 atgctggactccaaagcctccagagccagaagc-agcaaaactaatgagtgatgaactgg 1103
|||||||||||||||||||||||||||| ||| ||||||||||||||||||||||||||
Sbjct: 47619769 atgctggactccaaagcctccagagccaagagcaagcaaaactaatgagtgatgaactgg 47619710
Query: 1104 gctttaacactggccgttgaagaactcgccccctgtgt-cagttcttgcttctacaaatt 1162
|||| ||||||||||||||| ||||||||| ||||||| |||| |||||| ||||| |||
Sbjct: 47619709 gcttgaacactggccgttgaggaactcgccacctgtgtccagtccttgctcctacagatt 47619650
Query: 1163 ggaaagaccaatccaattgatcggtcaaactcgggctg 1200
|||| |||||||| |||||||| |||||||||||||||
Sbjct: 47619649 ggaaggaccaatcgaattgatctgtcaaactcgggctg 47619612
Score = 264 bits (133), Expect = 1e-67
Identities = 133/133 (100%)
Strand = Plus / Minus
Query: 735 gaatgataacttcaccatctgcatggagaccatcaccgcctgcctctggggaccactcag 794
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47621335 gaatgataacttcaccatctgcatggagaccatcaccgcctgcctctggggaccactcag 47621276
Query: 795 cctatgggtggtgatcgcctttctccgccagcagccccaccgctttgtcctccagctggt 854
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47621275 cctatgggtggtgatcgcctttctccgccagcagccccaccgctttgtcctccagctggt 47621216
Query: 855 ggtctctgtgggt 867
|||||||||||||
Sbjct: 47621215 ggtctctgtgggt 47621203
Score = 75.8 bits (38), Expect = 5e-11
Identities = 38/38 (100%)
Strand = Plus / Minus
Query: 698 ggaaggaatatgccaagggagacagccgatacatcctg 735
||||||||||||||||||||||||||||||||||||||
Sbjct: 47621503 ggaaggaatatgccaagggagacagccgatacatcctg 47621466
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 33,178,629
Number of extensions: 200
Number of successful extensions: 200
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 198
Number of HSP's successfully gapped: 5
Length of query: 1200
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 1179
Effective length of database: 2,808,413,156
Effective search space: 3311119110924
Effective search space used: 3311119110924
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)