BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-000326
(1697 letters)
Database: RefSeqBP
33,508 sequences; 16,112,626 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_899207.1| double substrate-specificity short chain dehydr... 275 6e-74
Alignment gi|XP_582373.2| PREDICTED: similar to Retinol dehydrogenase 11 ... 207 2e-53
Alignment gi|XP_870721.1| PREDICTED: similar to Retinol dehydrogenase 11 ... 193 4e-49
Alignment gi|XP_584629.2| PREDICTED: similar to Retinol dehydrogenase 13,... 147 3e-35
Alignment gi|XP_582319.2| PREDICTED: similar to retinol dehydrogenase 14 ... 122 9e-28
Alignment gi|XP_592820.2| PREDICTED: similar to retinol dehydrogenase 11 ... 120 4e-27
Alignment gi|XP_584642.2| PREDICTED: similar to retinol dehydrogenase 11 ... 107 4e-23
Alignment gi|XP_583859.2| PREDICTED: similar to short chain dehydrogenase... 100 3e-21
Alignment gi|XP_584810.2| PREDICTED: similar to retinol dehydrogenase 13 ... 71 3e-12
Alignment gi|XP_591168.2| PREDICTED: similar to dehydrogenase/reductase (... 64 3e-10
>ref|NP_899207.1| double substrate-specificity short chain dehydrogenase/reductase 2
[Bos taurus]
Length = 316
Score = 275 bits (704), Expect = 6e-74
Identities = 142/156 (91%), Positives = 147/156 (94%)
Frame = +2
Query: 149 MLAVLGLLTSFLFFLYVTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELA 328
ML VLGLLTSFL FLYV APSIRKFFAGGVCRT++QL GKVVVITGANTGIGKETARELA
Sbjct: 1 MLVVLGLLTSFLSFLYVIAPSIRKFFAGGVCRTDVQLFGKVVVITGANTGIGKETARELA 60
Query: 329 RRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLH 508
RRGARVYIACRDVLKGESAASEI+ADTKNSQVLVRKLDLSDTKSIRAFAEGFL EEKQLH
Sbjct: 61 RRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDTKSIRAFAEGFLAEEKQLH 120
Query: 509 ILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTY 616
ILINNAGVMLCPYSKTADGFETHL VNHLG + T+
Sbjct: 121 ILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTH 156
Score = 192 bits (489), Expect = 5e-49
Identities = 97/123 (78%), Positives = 103/123 (83%), Gaps = 1/123 (0%)
Frame = +2
Query: 521 NAGVMLCPYSKTADG-FETHLGVNHLGTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFSR 697
N G C +SK A+ F L GTGVTTYAVHPGIV+S+LVRHSFLLCLLWRLFS
Sbjct: 195 NLGFAYC-HSKLANVLFTRELAKRLKGTGVTTYAVHPGIVRSKLVRHSFLLCLLWRLFSP 253
Query: 698 FLKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
FLKT EGAQTSLHCALAEGLEPLSGKYFSDCK+ WVSPRARNNKTAERLWNVSCELLGI
Sbjct: 254 FLKTTWEGAQTSLHCALAEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLWNVSCELLGI 313
Query: 878 QWE 886
+WE
Sbjct: 314 RWE 316
>ref|XP_582373.2| PREDICTED: similar to Retinol dehydrogenase 11 (Retinal reductase
1) (RalR1) (Prostate short-chain dehydrogenase/reductase
1) (Androgen-regulated short-chain
dehydrogenase/reductase 1) (HCV core-binding protein
HCBP12) isoform 1 [Bos taurus]
Length = 308
Score = 207 bits (527), Expect = 2e-53
Identities = 129/282 (45%), Positives = 156/282 (55%), Gaps = 65/282 (23%)
Frame = +2
Query: 227 AGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRAD 406
+ GVC + +QLPGKV V+TGAN GIGKETA+ELARRGARVY+ACRDV GE A EI+
Sbjct: 3 SSGVCTSTIQLPGKVAVVTGANAGIGKETAKELARRGARVYLACRDVQNGELVAREIQMM 62
Query: 407 TKNSQVLVRKLD--------------LSDTKSIR----------------AFAEGFLTEE 496
T N QVLVRKLD L D K I A G+L
Sbjct: 63 TGNQQVLVRKLDLADTKSIRAFAKRFLEDHKYIHLINSVGFPGSSAGKDSACNTGYLGHF 122
Query: 497 KQLHILINN----------------------------------AGVMLCPYSKTADG-FE 571
H+L+ +G+ C +SK A+ F
Sbjct: 123 LLTHLLLEKLEESAPSRVVNVSSLAHLLGRIHFHNLQGEKFYQSGLAYC-HSKLANILFT 181
Query: 572 THLGVNHLGTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFSRFLKTAREGAQTSLHCALA 751
L G+GVT Y+VHPG V SELVRHS L+ +W +FS F+KT ++GAQTSL+CAL
Sbjct: 182 QELARRLKGSGVTVYSVHPGTVNSELVRHSALMRWIWWIFSFFIKTPQQGAQTSLYCALT 241
Query: 752 EGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
EGLE LSG +FSDC AWVS +ARN A RLW+VSC+LLGI
Sbjct: 242 EGLEVLSGNHFSDCHVAWVSAQARNETVARRLWDVSCDLLGI 283
>ref|XP_870721.1| PREDICTED: similar to Retinol dehydrogenase 11 (Retinal reductase
1) (RalR1) (Prostate short-chain dehydrogenase/reductase
1) (Androgen-regulated short-chain
dehydrogenase/reductase 1) (HCV core-binding protein
HCBP12) isoform 2 [Bos taurus]
Length = 314
Score = 193 bits (490), Expect = 4e-49
Identities = 123/289 (42%), Positives = 154/289 (53%), Gaps = 72/289 (24%)
Frame = +2
Query: 227 AGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIR-- 400
+ GVC + +QLPGKV V+TGAN GIGKETA+ELARRGARVY+ACRDV GE A EI+
Sbjct: 3 SSGVCTSTIQLPGKVAVVTGANAGIGKETAKELARRGARVYLACRDVQNGELVAREIQMM 62
Query: 401 -------------ADTKNSQVLVRKLDLSDTKSIR----------------------AFA 475
ADTK+ + ++ L D K I A
Sbjct: 63 TGNQQVLVRKLDLADTKSIRAFAKRF-LEDHKYIHLINSSPHFLICGFPGSSAGKDSACN 121
Query: 476 EGFLTEEKQLHILINN----------------------------------AGVMLCPYSK 553
G+L H+L+ +G+ C +SK
Sbjct: 122 TGYLGHFLLTHLLLEKLEESAPSRVVNVSSLAHLLGRIHFHNLQGEKFYQSGLAYC-HSK 180
Query: 554 TADG-FETHLGVNHLGTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFSRFLKTAREGAQT 730
A+ F L G+GVT Y+VHPG V SELVRHS L+ +W +FS F+KT ++GAQT
Sbjct: 181 LANILFTQELARRLKGSGVTVYSVHPGTVNSELVRHSALMRWIWWIFSFFIKTPQQGAQT 240
Query: 731 SLHCALAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
SL+CAL EGLE LSG +FSDC AWVS +ARN A RLW+VSC+LLGI
Sbjct: 241 SLYCALTEGLEVLSGNHFSDCHVAWVSAQARNETVARRLWDVSCDLLGI 289
>ref|XP_584629.2| PREDICTED: similar to Retinol dehydrogenase 13, partial [Bos
taurus]
Length = 148
Score = 147 bits (370), Expect = 3e-35
Identities = 72/128 (56%), Positives = 91/128 (71%)
Frame = +2
Query: 212 IRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAAS 391
++ F AGG C + +PGK V++TGANTGIGK+TA ELA+RG + +ACRD+ K E+AA
Sbjct: 21 LKDFVAGGACPSKATIPGKTVIVTGANTGIGKQTALELAKRGGNIILACRDMEKCEAAAK 80
Query: 392 EIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFE 571
EIR +T N +V R LDL+ KSIR FA EE+ +HILINNA VM CP+ T DGFE
Sbjct: 81 EIRGETLNHRVNARHLDLASLKSIREFAAKVTEEEEHVHILINNAAVMRCPHWTTEDGFE 140
Query: 572 THLGVNHL 595
LGVN+L
Sbjct: 141 MQLGVNYL 148
>ref|XP_582319.2| PREDICTED: similar to retinol dehydrogenase 14 (all-trans and
9-cis) [Bos taurus]
Length = 420
Score = 122 bits (306), Expect = 9e-28
Identities = 78/190 (41%), Positives = 106/190 (55%), Gaps = 18/190 (9%)
Frame = +2
Query: 83 RRSPSGVEVPFAEAAASKAVETMLAVLGLLTSFLFFLYVTAPSIRKFFAGGVCRTNMQ-- 256
R+S +G P A A AV T A+L L L+ + R+F V R +
Sbjct: 70 RKSRAGAGGPSAPVAM--AVGTAAALLAALGGILWL------AARRFVGSSVQRLHQGRD 121
Query: 257 ---LPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADT------ 409
+ GK V+ITGAN+G+G+ TA EL R GARV + CRD + E AA ++R +
Sbjct: 122 SGLMRGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRERAEEAAGQLRREVCPAGGP 181
Query: 410 ---KNS----QVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGF 568
NS +++V++LDL+ S+R+F + L EE +L +LINNAGV CPY KT DGF
Sbjct: 182 DSGPNSGGAGELVVKELDLASLSSVRSFCQEMLQEEPRLDVLINNAGVFQCPYMKTEDGF 241
Query: 569 ETHLGVNHLG 598
E GVNHLG
Sbjct: 242 EMQFGVNHLG 251
Score = 78.2 bits (191), Expect = 2e-14
Identities = 40/98 (40%), Positives = 61/98 (62%), Gaps = 4/98 (4%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFS----RFLKTAREGAQTSLHCALAEGLE 763
GT VT +HPGIV++ L RH + L+ LF+ F KT EGAQT+++ A + +E
Sbjct: 321 GTSVTVNVLHPGIVRTNLGRHIHIPLLVRPLFNLVSWAFFKTPEEGAQTAVYLASSPEVE 380
Query: 764 PLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
+SG+YF DCK + P+A + A +LW++S ++GI
Sbjct: 381 GVSGRYFGDCKEEELLPKAMDESVARKLWDISEVMVGI 418
>ref|XP_592820.2| PREDICTED: similar to retinol dehydrogenase 11 (predicted) [Bos
taurus]
Length = 377
Score = 120 bits (300), Expect = 4e-27
Identities = 72/171 (42%), Positives = 102/171 (59%), Gaps = 3/171 (1%)
Frame = +2
Query: 140 VETMLAVLGLLTSFLFFLY---VTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKE 310
+E +L +GLL +Y V AP CR L G+ V+TGAN+GIGK
Sbjct: 1 MEALLLGVGLLLGAYVLVYYNLVKAPP---------CRGLASLRGRTAVVTGANSGIGKM 51
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLT 490
TA ELARRGARV +ACR +GE+AA ++R ++ N++V+ LDL+ S+RAFA FL+
Sbjct: 52 TALELARRGARVVLACRSRERGEAAAFDLRQESGNNEVIFMALDLASLASVRAFATAFLS 111
Query: 491 EEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTYAVHPGIVQS 643
E +L ILI+NAG+ C +T + F L VNH+G + T+ + P + S
Sbjct: 112 SEPRLDILIHNAGISSC--GRTREPFNLLLRVNHIGPFLLTHLLLPRLKTS 160
Score = 91.7 bits (226), Expect = 2e-18
Identities = 53/107 (49%), Positives = 66/107 (61%), Gaps = 3/107 (2%)
Frame = +2
Query: 566 FETHLGVNHLGTGVTTYAVHPGIVQSEL-VRH--SFLLCLLWRLFSRFLKTAREGAQTSL 736
F L GTGVT YA HPG V SEL +RH +L LL L L+ R GAQT L
Sbjct: 207 FARELATQLEGTGVTCYAAHPGPVNSELFLRHVPGWLRPLLRPLAWLVLRAPRGGAQTPL 266
Query: 737 HCALAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
+CAL EG+EPLSG+YF++C V P AR+++ A RLW S +L G+
Sbjct: 267 YCALQEGIEPLSGRYFANCHVEEVPPAARDDRAAHRLWEASRKLAGL 313
>ref|XP_584642.2| PREDICTED: similar to retinol dehydrogenase 11 (predicted) [Bos
taurus]
Length = 330
Score = 107 bits (266), Expect = 4e-23
Identities = 54/132 (40%), Positives = 85/132 (64%)
Frame = +2
Query: 218 KFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEI 397
KF+ + + L GK V+TGAN+GIGK ++ELA RGARV +ACR +G+ A +EI
Sbjct: 34 KFYLWDLQHCSTDLTGKTAVVTGANSGIGKAVSQELAHRGARVILACRSRERGQQALAEI 93
Query: 398 RADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETH 577
+A +K++++L+ ++DLS SIR+FA+ L E ++H+L+NNA V P + T +G +
Sbjct: 94 QATSKSNRLLLGEVDLSSMASIRSFAQRLLQECPEIHLLVNNAAVCGFPTTLTPEGLDLT 153
Query: 578 LGVNHLGTGVTT 613
N+ G + T
Sbjct: 154 FATNYTGPFLLT 165
Score = 60.1 bits (144), Expect = 5e-09
Identities = 35/96 (36%), Positives = 57/96 (59%), Gaps = 2/96 (2%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRH-SFLLCLLWRLFSRFLKTAREGAQTSLHCALAEGLEPLS 772
GTGVT +V PG+V +++++H S+ L+ L S F K +++GA L+ +LA+ L+ +S
Sbjct: 231 GTGVTVNSVDPGVVYTKIMKHFSWSYRFLFWLLSFFFKDSKQGAVPVLYLSLAKELDGIS 290
Query: 773 GKYF-SDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
GK+F S C A++ A+ LWN S L +
Sbjct: 291 GKHFSSSCVITLPPEAAQDPHVAQSLWNTSVRLTNL 326
>ref|XP_583859.2| PREDICTED: similar to short chain dehydrogenase/reductase [Bos
taurus]
Length = 317
Score = 100 bits (250), Expect = 3e-21
Identities = 53/126 (42%), Positives = 77/126 (61%)
Frame = +2
Query: 251 MQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLV 430
+Q+PG+ ++TG N+GIGK TA E+A+RG V++ CRD + E A +EI ++ N + +
Sbjct: 36 VQVPGRAFMVTGGNSGIGKATAMEIAKRGGTVHLVCRDHSRAEGAKAEIIRESGNQNIFL 95
Query: 431 RKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVT 610
+DLS KS+ F E F +E L++LINNAG M+ T DG E + N LG V
Sbjct: 96 HIVDLSLPKSVWKFVENF-KQEHTLNVLINNAGCMVNKRELTEDGLEKNFATNTLGVYVL 154
Query: 611 TYAVHP 628
T A+ P
Sbjct: 155 TTALIP 160
>ref|XP_584810.2| PREDICTED: similar to retinol dehydrogenase 13 (all-trans and
9-cis) [Bos taurus]
Length = 223
Score = 70.9 bits (172), Expect = 3e-12
Identities = 40/101 (39%), Positives = 59/101 (58%), Gaps = 6/101 (5%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHS------FLLCLLWRLFSRFLKTAREGAQTSLHCALAEG 757
GTGVT A+HPG+ ++EL RH+ F L +F +K+ AQ S++ A+AE
Sbjct: 108 GTGVTVNALHPGVARTELGRHTGMHSSAFSSFTLGPIFWLLVKSPELAAQPSVYLAVAEE 167
Query: 758 LEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGIQ 880
LE +SGKYF K +P A + + A+RLW S L+G++
Sbjct: 168 LEGVSGKYFDVLKEKPPAPEAEDEEVAKRLWAESVRLVGLE 208
>ref|XP_591168.2| PREDICTED: similar to dehydrogenase/reductase (SDR family) X-linked
[Bos taurus]
Length = 251
Score = 64.3 bits (155), Expect = 3e-10
Identities = 32/74 (43%), Positives = 45/74 (60%)
Frame = +2
Query: 440 DLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTYA 619
DL+ +SIR F + F ++ LH+L+NNAGVM+ P T DGFE H GVN+LG + T
Sbjct: 11 DLASMRSIREFVQTFKMKKLPLHVLVNNAGVMMVPQRTTEDGFEEHFGVNYLGHFLLTNL 70
Query: 620 VHPGIVQSELVRHS 661
+ + +S HS
Sbjct: 71 LLDTLQESGAPGHS 84
Database: RefSeqBP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 16,112,626
Number of sequences in database: 33,508
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,227,444
Number of Sequences: 33508
Number of extensions: 1805966
Number of successful extensions: 7177
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 5556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7144
length of database: 16,112,626
effective HSP length: 107
effective length of database: 12,527,270
effective search space used: 5737489660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqSP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-000326
(1697 letters)
Database: RefSeqSP
1040 sequences; 434,620 total letters
Searching...done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_999238.1| 20-beta-hydroxysteroid dehydrogenase [Sus scrofa] 56 2e-09
>ref|NP_999238.1| 20-beta-hydroxysteroid dehydrogenase [Sus scrofa]
Length = 289
Score = 56.2 bits (134), Expect = 2e-09
Identities = 32/90 (35%), Positives = 54/90 (60%), Gaps = 1/90 (1%)
Frame = +2
Query: 266 KVVVITGANTGIGKETARELARRGAR-VYIACRDVLKGESAASEIRADTKNSQVLVRKLD 442
+V ++TGAN GIG R+L R+ A V + RDV +G++A +++A+ + + +LD
Sbjct: 6 RVALVTGANKGIGFAIVRDLCRQFAGDVVLTARDVARGQAAVKQLQAEGLSPRF--HQLD 63
Query: 443 LSDTKSIRAFAEGFLTEEKQLHILINNAGV 532
+ D +SIRA + E L +L+NNA +
Sbjct: 64 IIDLQSIRALCDFLRKEYGGLDVLVNNAAI 93
Database: RefSeqSP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 434,620
Number of sequences in database: 1040
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,682,536
Number of Sequences: 1040
Number of extensions: 46885
Number of successful extensions: 162
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 434,620
effective HSP length: 82
effective length of database: 349,340
effective search space used: 168731220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqHP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-000326
(1697 letters)
Database: RefSeqHP
39,411 sequences; 17,774,539 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_689656.1| retinol dehydrogenase 12 (all-trans and 9-cis) ... 315 1e-85
Alignment gi|NP_057110.2| androgen-regulated short-chain dehydrogenase/re... 224 1e-58
Alignment gi|NP_065956.1| retinol dehydrogenase 14 (all-trans and 9-cis) ... 124 2e-28
Alignment gi|NP_660160.1| dehydrogenase/reductase (SDR family) X-linked [... 100 3e-21
Alignment gi|NP_057457.1| WW domain-containing oxidoreductase isoform 1 [... 100 3e-21
Alignment gi|NP_653284.1| hypothetical protein LOC147015 [Homo sapiens] 94 5e-19
Alignment gi|XP_371503.3| PREDICTED: similar to short-chain dehydrogenase... 93 6e-19
Alignment gi|XP_947127.1| PREDICTED: similar to short-chain dehydrogenase... 93 6e-19
Alignment gi|NP_612421.1| retinol dehydrogenase 13 (all-trans and 9-cis) ... 85 2e-16
Alignment gi|NP_057113.1| dehydrogenase/reductase (SDR family) member 7 [... 64 4e-10
>ref|NP_689656.1| retinol dehydrogenase 12 (all-trans and 9-cis) [Homo sapiens]
Length = 316
Score = 315 bits (806), Expect = 1e-85
Identities = 185/322 (57%), Positives = 202/322 (62%), Gaps = 76/322 (23%)
Frame = +2
Query: 149 MLAVLGLLTSFLFFLYVTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELA 328
ML LGLLTSF FLY+ APSIRKFFAGGVCRTN+QLPGKVVVITGANTGIGKETARELA
Sbjct: 1 MLVTLGLLTSFFSFLYMVAPSIRKFFAGGVCRTNVQLPGKVVVITGANTGIGKETARELA 60
Query: 329 RRGARVYIACRDVLKGESAASEIR---------------ADTKNSQVLVRKLDLSDTKSI 463
RGARVYIACRDVLKGESAASEIR +DTK+ + L++ K +
Sbjct: 61 SRGARVYIACRDVLKGESAASEIRVDTKNSQVLVRKLDLSDTKSIRAFAEGF-LAEEKQL 119
Query: 464 RAF--------------AEGFLTEEKQLHILINNAGVMLCPY------------------ 547
A+GF T H+ +N+ G L Y
Sbjct: 120 HILINNAGVMMCPYSKTADGFET-----HLGVNHLGHFLLTYLLLEQLKVSAPARVVNVS 174
Query: 548 ----------------------------SKTADG-FETHLGVNHLGTGVTTYAVHPGIVQ 640
SK A+ F L GTGVTTYAVHPG+V+
Sbjct: 175 SVAHHIGKIPFHDLQSEKRYSRGFAYCHSKLANVLFTRELAKRLQGTGVTTYAVHPGVVR 234
Query: 641 SELVRHSFLLCLLWRLFSRFLKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRAWVSPRA 820
SELVRHS LLCLLWRLFS F+KTAREGAQTSLHCALAEGLEPLSGKYFSDCKR WVSPRA
Sbjct: 235 SELVRHSSLLCLLWRLFSPFVKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRTWVSPRA 294
Query: 821 RNNKTAERLWNVSCELLGIQWE 886
RNNKTAERLWNVSCELLGI+WE
Sbjct: 295 RNNKTAERLWNVSCELLGIRWE 316
>ref|NP_057110.2| androgen-regulated short-chain dehydrogenase/reductase 1 [Homo
sapiens]
Length = 318
Score = 224 bits (572), Expect = 1e-58
Identities = 114/159 (71%), Positives = 130/159 (81%)
Frame = +2
Query: 140 VETMLAVLGLLTSFLFFLYVTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETAR 319
VE M +L LL FL LY+ AP IRK + GVC + +QLPGKVVV+TGANTGIGKETA+
Sbjct: 2 VELMFPLLLLLLPFL--LYMAAPQIRKMLSSGVCTSTVQLPGKVVVVTGANTGIGKETAK 59
Query: 320 ELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEK 499
ELA+RGARVY+ACRDV KGE A EI+ T N QVLVRKLDLSDTKSIRAFA+GFL EEK
Sbjct: 60 ELAQRGARVYLACRDVEKGELVAKEIQTTTGNQQVLVRKLDLSDTKSIRAFAKGFLAEEK 119
Query: 500 QLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTY 616
LH+LINNAGVM+CPYSKTADGFE H+GVNHLG + T+
Sbjct: 120 HLHVLINNAGVMMCPYSKTADGFEMHIGVNHLGHFLLTH 158
Score = 139 bits (351), Expect = 6e-33
Identities = 73/120 (60%), Positives = 88/120 (73%), Gaps = 1/120 (0%)
Frame = +2
Query: 521 NAGVMLCPYSKTADG-FETHLGVNHLGTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFSR 697
NAG+ C +SK A+ F L G+GVTTY+VHPG VQSELVRHS + +W LFS
Sbjct: 197 NAGLAYC-HSKLANILFTQELARRLKGSGVTTYSVHPGTVQSELVRHSSFMRWMWWLFSF 255
Query: 698 FLKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
F+KT ++GAQTSLHCAL EGLE LSG +FSDC AWVS +ARN A RLW+VSC+LLG+
Sbjct: 256 FIKTPQQGAQTSLHCALTEGLEILSGNHFSDCHVAWVSVQARNETIARRLWDVSCDLLGL 315
>ref|NP_065956.1| retinol dehydrogenase 14 (all-trans and 9-cis) [Homo sapiens]
Length = 336
Score = 124 bits (311), Expect = 2e-28
Identities = 71/169 (42%), Positives = 98/169 (57%), Gaps = 15/169 (8%)
Frame = +2
Query: 137 AVETMLAVLGLLTSFLFFLY--VTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKE 310
AV T AVL L L+ P +++ GG + GK V+ITGAN+G+G+
Sbjct: 2 AVATAAAVLAALGGALWLAARRFVGPRVQRLRRGG---DPGLMHGKTVLITGANSGLGRA 58
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNS-------------QVLVRKLDLSD 451
TA EL R GARV + CRD + E AA ++R + + + +++VR+LDL+
Sbjct: 59 TAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPEPGVSGVGELIVRELDLAS 118
Query: 452 TKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLG 598
+S+RAF + L EE +L +LINNAG+ CPY KT DGFE GVNHLG
Sbjct: 119 LRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLG 167
Score = 77.8 bits (190), Expect = 3e-14
Identities = 40/98 (40%), Positives = 61/98 (62%), Gaps = 4/98 (4%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFS----RFLKTAREGAQTSLHCALAEGLE 763
GT VT +HPGIV++ L RH + L+ LF+ F KT EGAQTS++ A + +E
Sbjct: 237 GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPLFNLVSWAFFKTPVEGAQTSIYLASSPEVE 296
Query: 764 PLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
+SG+YF DCK + P+A + A +LW++S ++G+
Sbjct: 297 GVSGRYFGDCKEEELLPKAMDESVARKLWDISEVMVGL 334
>ref|NP_660160.1| dehydrogenase/reductase (SDR family) X-linked [Homo sapiens]
Length = 330
Score = 100 bits (250), Expect = 3e-21
Identities = 55/134 (41%), Positives = 77/134 (57%)
Frame = +2
Query: 260 PGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKL 439
P +V ++TG GIG TA+ LAR G V IA + K + S+I+ +T N +V
Sbjct: 42 PDRVAIVTGGTDGIGYSTAKHLARLGMHVIIAGNNDSKAKQVVSKIKEETLNDKVEFLYC 101
Query: 440 DLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTYA 619
DL+ SIR F + F ++ LH+LINNAGVM+ P KT DGFE H G+N+LG + T
Sbjct: 102 DLASMTSIRQFVQKFKMKKIPLHVLINNAGVMMVPQRKTRDGFEEHFGLNYLGHFLLTNL 161
Query: 620 VHPGIVQSELVRHS 661
+ + +S HS
Sbjct: 162 LLDTLKESGSPGHS 175
Score = 57.8 bits (138), Expect = 3e-08
Identities = 35/100 (35%), Positives = 51/100 (51%), Gaps = 6/100 (6%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHSFLLCLL------WRLFSRFLKTAREGAQTSLHCALAEG 757
G+ VT V PG+V ++L +H F L W LF KT EGA TS++ A+
Sbjct: 230 GSHVTANVVDPGVVNTDLYKHVFWATRLAKKLLGWLLF----KTPDEGAWTSIYAAVTPE 285
Query: 758 LEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
LE + G+Y + K N K ++LW+ SCE+ G+
Sbjct: 286 LEGVGGRYLYNEKETKSLHVTYNQKLQQQLWSKSCEMTGV 325
>ref|NP_057457.1| WW domain-containing oxidoreductase isoform 1 [Homo sapiens]
Length = 414
Score = 100 bits (250), Expect = 3e-21
Identities = 55/137 (40%), Positives = 77/137 (56%), Gaps = 4/137 (2%)
Frame = +2
Query: 200 TAPSIRKFFAGGVCRTNM----QLPGKVVVITGANTGIGKETARELARRGARVYIACRDV 367
T P+ R+ + G + GKVVV+TGAN+GIG ETA+ A GA V +ACR++
Sbjct: 99 TKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNM 158
Query: 368 LKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPY 547
+ A S I + ++V LDL+ +S++ FAE F + LH+L+ NA P+
Sbjct: 159 ARASEAVSRILEEWHKAKVEAMTLDLALLRSVQHFAEAFKAKNVPLHVLVCNAATFALPW 218
Query: 548 SKTADGFETHLGVNHLG 598
S T DG ET VNHLG
Sbjct: 219 SLTKDGLETTFQVNHLG 235
Score = 69.3 bits (168), Expect = 9e-12
Identities = 37/91 (40%), Positives = 57/91 (62%), Gaps = 1/91 (1%)
Frame = +2
Query: 602 GVTTYAVHPG-IVQSELVRHSFLLCLLWRLFSRFLKTAREGAQTSLHCALAEGLEPLSGK 778
GVT+ AVHPG ++ S + R ++ LL+ L F K+ ++GA T+++CA LE L G
Sbjct: 315 GVTSNAVHPGNMMYSNIHRSWWVYTLLFTLARPFTKSMQQGAATTVYCAAVPELEGLGGM 374
Query: 779 YFSDCKRAWVSPRARNNKTAERLWNVSCELL 871
YF++C R SP A++ +TA LW +S L+
Sbjct: 375 YFNNCCRCMPSPEAQSEETARTLWALSERLI 405
>ref|NP_653284.1| hypothetical protein LOC147015 [Homo sapiens]
Length = 327
Score = 93.6 bits (231), Expect = 5e-19
Identities = 49/106 (46%), Positives = 71/106 (66%)
Frame = +2
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLT 490
TA ELARRGARV +ACR +GE+AA ++R ++ N++V+ LDL+ S+RAFA FL+
Sbjct: 2 TALELARRGARVVLACRSQERGEAAAFDLRQESGNNEVIFMALDLASLASVRAFATAFLS 61
Query: 491 EEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTYAVHP 628
E +L ILI+NAG+ C +T + F L VNH+G + T+ + P
Sbjct: 62 SEPRLDILIHNAGISSC--GRTREAFNLLLRVNHIGPFLLTHLLLP 105
Score = 90.1 bits (222), Expect = 5e-18
Identities = 55/123 (44%), Positives = 70/123 (56%), Gaps = 3/123 (2%)
Frame = +2
Query: 599 TGVTTYAVHPGIVQSEL-VRH--SFLLCLLWRLFSRFLKTAREGAQTSLHCALAEGLEPL 769
TGVT YA HPG V SEL +RH +L LL L L+ R GAQT L+CAL EG+EPL
Sbjct: 168 TGVTCYAAHPGPVNSELFLRHVPGWLRPLLRPLAWLVLRAPRGGAQTPLYCALQEGIEPL 227
Query: 770 SGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGIQWE*SCWKMRGCIKPGPGHNADRG 949
SG+YF++C V P AR+++ A RLW S L G+ GPG +A+
Sbjct: 228 SGRYFANCHVEEVPPAARDDRAAHRLWEASKRLAGL---------------GPGEDAEPD 272
Query: 950 DGP 958
+ P
Sbjct: 273 EDP 275
>ref|XP_371503.3| PREDICTED: similar to short-chain dehydrogenase/reductase 1 [Homo
sapiens]
Length = 891
Score = 93.2 bits (230), Expect = 6e-19
Identities = 47/120 (39%), Positives = 79/120 (65%), Gaps = 1/120 (0%)
Frame = +2
Query: 257 LPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRK 436
L GK+ ++TGAN+GIGK +++LAR GA+V + C+ G+ A +EI+A + ++++L+ +
Sbjct: 600 LTGKIAIVTGANSGIGKVVSQDLARCGAQVILTCQSRECGQQALAEIQAASNSNRLLLGE 659
Query: 437 LDLSDTKSIRAFAEGFLTEEKQLHILINNAGVM-LCPYSKTADGFETHLGVNHLGTGVTT 613
+DLS SIR+FA L E ++H+L+NNAGV + P + T G + N++G + T
Sbjct: 660 VDLSSMTSIRSFARRLLQENPEIHLLVNNAGVSGMIPKTLTPGGLDLTFVTNYVGPFLLT 719
>ref|XP_947127.1| PREDICTED: similar to short-chain dehydrogenase/reductase 1 [Homo
sapiens]
Length = 916
Score = 93.2 bits (230), Expect = 6e-19
Identities = 47/120 (39%), Positives = 79/120 (65%), Gaps = 1/120 (0%)
Frame = +2
Query: 257 LPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRK 436
L GK+ ++TGAN+GIGK +++LAR GA+V + C+ G+ A +EI+A + ++++L+ +
Sbjct: 625 LTGKIAIVTGANSGIGKVVSQDLARCGAQVILTCQSRECGQQALAEIQAASNSNRLLLGE 684
Query: 437 LDLSDTKSIRAFAEGFLTEEKQLHILINNAGVM-LCPYSKTADGFETHLGVNHLGTGVTT 613
+DLS SIR+FA L E ++H+L+NNAGV + P + T G + N++G + T
Sbjct: 685 VDLSSMTSIRSFARRLLQENPEIHLLVNNAGVSGMIPKTLTPGGLDLTFVTNYVGPFLLT 744
>ref|NP_612421.1| retinol dehydrogenase 13 (all-trans and 9-cis) [Homo sapiens]
Length = 260
Score = 84.7 bits (208), Expect = 2e-16
Identities = 43/76 (56%), Positives = 52/76 (68%)
Frame = +2
Query: 371 KGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYS 550
K E+AA +IR +T N V R LDL+ KSIR FA + EE+++ ILINNAGVM CP+
Sbjct: 3 KCEAAAKDIRGETLNHHVNARHLDLASLKSIREFAAKIIEEEERVDILINNAGVMRCPHW 62
Query: 551 KTADGFETHLGVNHLG 598
T DGFE GVNHLG
Sbjct: 63 TTEDGFEMQFGVNHLG 78
Score = 68.6 bits (166), Expect = 2e-11
Identities = 38/101 (37%), Positives = 57/101 (56%), Gaps = 6/101 (5%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHS------FLLCLLWRLFSRFLKTAREGAQTSLHCALAEG 757
G+GVT A+HPG+ ++EL RH+ F L +F +K+ AQ S + A+AE
Sbjct: 149 GSGVTVNALHPGVARTELGRHTGIHGSTFSSTTLGPIFWLLVKSPELAAQPSTYLAVAEE 208
Query: 758 LEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGIQ 880
L +SGKYF K+ +P A + + A RLW S L+G++
Sbjct: 209 LADVSGKYFDGLKQKAPAPEAEDEEVARRLWAESARLVGLE 249
>ref|NP_057113.1| dehydrogenase/reductase (SDR family) member 7 [Homo sapiens]
Length = 339
Score = 63.9 bits (154), Expect = 4e-10
Identities = 51/169 (30%), Positives = 82/169 (48%), Gaps = 5/169 (2%)
Frame = +2
Query: 149 MLAVLGLLTSFLFFLYVTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELA 328
+ A+L LL L FL + R +L VV +TGA++GIG+E A +L+
Sbjct: 12 LCALLLLLVQLLRFLRADGDLTLLWAEWQGRRPEWELTDMVVWVTGASSGIGEELAYQLS 71
Query: 329 RRGARVYIACRDVLKGESAASEI--RADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQ 502
+ G + ++ R V + E + K +LV LDL+DT S A + L E +
Sbjct: 72 KLGVSLVLSARRVHELERVKRRCLENGNLKEKDILVLPLDLTDTGSHEAATKAVLQEFGR 131
Query: 503 LHILINNAGV---MLCPYSKTADGFETHLGVNHLGTGVTTYAVHPGIVQ 640
+ IL+NN G+ LC + D + + +N+LGT T V P +++
Sbjct: 132 IDILVNNGGMSQRSLC-MDTSLDVYRKLIELNYLGTVSLTKCVLPHMIE 179
Database: RefSeqHP
Posted date: Aug 2, 2006 12:57 AM
Number of letters in database: 17,774,539
Number of sequences in database: 39,411
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,983,337
Number of Sequences: 39411
Number of extensions: 2128774
Number of successful extensions: 8506
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 6525
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8458
length of database: 17,774,539
effective HSP length: 107
effective length of database: 13,557,562
effective search space used: 6209363396
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqCP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-000326
(1697 letters)
Database: RefSeqCP
33,732 sequences; 19,266,565 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_547866.2| PREDICTED: similar to retinol dehydrogenase 12 ... 250 3e-66
Alignment gi|XP_854354.1| PREDICTED: similar to Retinol dehydrogenase 11 ... 202 8e-52
Alignment gi|XP_854127.1| PREDICTED: similar to Retinol dehydrogenase 13 ... 152 1e-36
Alignment gi|XP_857582.1| PREDICTED: similar to retinol dehydrogenase 14 ... 125 2e-28
Alignment gi|XP_540096.2| PREDICTED: similar to retinol dehydrogenase 14 ... 122 8e-28
Alignment gi|XP_548293.2| PREDICTED: similar to retinol dehydrogenase 11 ... 116 8e-26
Alignment gi|XP_533000.2| PREDICTED: similar to Retinol dehydrogenase 12 ... 103 7e-22
Alignment gi|XP_852623.1| PREDICTED: similar to WW domain-containing oxid... 103 7e-22
Alignment gi|XP_852222.1| PREDICTED: similar to dehydrogenase/reductase (... 92 2e-18
Alignment gi|XP_857503.1| PREDICTED: similar to retinol dehydrogenase 14 ... 72 1e-12
>ref|XP_547866.2| PREDICTED: similar to retinol dehydrogenase 12 (all-trans and
9-cis) [Canis familiaris]
Length = 303
Score = 250 bits (638), Expect = 3e-66
Identities = 127/147 (86%), Positives = 135/147 (91%)
Frame = +2
Query: 215 RKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASE 394
RKFFAGGVCR+N+QLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASE
Sbjct: 10 RKFFAGGVCRSNVQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASE 69
Query: 395 IRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFET 574
IRADTKNSQVLVRKLDLSDTKSIRAFAEGFL EEKQLHILINNAGVM+CPYSKTADGFET
Sbjct: 70 IRADTKNSQVLVRKLDLSDTKSIRAFAEGFLAEEKQLHILINNAGVMMCPYSKTADGFET 129
Query: 575 HLGVNHLGTGVTTYAVHPGIVQSELVR 655
HLGVNHLG + T+ + + +S R
Sbjct: 130 HLGVNHLGHFLLTHLLLERLKESTPAR 156
Score = 194 bits (492), Expect = 3e-49
Identities = 91/107 (85%), Positives = 97/107 (90%)
Frame = +2
Query: 566 FETHLGVNHLGTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFSRFLKTAREGAQTSLHCA 745
F L GTGVTTYAVHPG+V SELVRHSFLLCLLWR+FS F+K+AREGAQTSLHCA
Sbjct: 197 FTRELAKRLQGTGVTTYAVHPGVVSSELVRHSFLLCLLWRIFSPFVKSAREGAQTSLHCA 256
Query: 746 LAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGIQWE 886
LAEGLEPLSGKYFSDCKRAWVSPRAR+NKTAERLWNVSCELLGIQW+
Sbjct: 257 LAEGLEPLSGKYFSDCKRAWVSPRARDNKTAERLWNVSCELLGIQWD 303
>ref|XP_854354.1| PREDICTED: similar to Retinol dehydrogenase 11 (Retinal reductase
1) (RalR1) (Prostate short-chain dehydrogenase/reductase
1) (Androgen-regulated short-chain
dehydrogenase/reductase 1) (HCV core-binding protein
HCBP12) [Canis familiaris]
Length = 337
Score = 202 bits (514), Expect = 8e-52
Identities = 98/135 (72%), Positives = 114/135 (84%)
Frame = +2
Query: 212 IRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAAS 391
+ K + GVC + +QLPGKV V+TGANTGIGKETA+ELA+RGARVY+ACRDVLKGE A
Sbjct: 43 LMKILSRGVCTSTVQLPGKVAVVTGANTGIGKETAKELAQRGARVYLACRDVLKGELVAR 102
Query: 392 EIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFE 571
EI+ T N QVLVRKLDL+DTKSIRAFA+GFL EEK LHILINNAGVM+CPY+KT DGFE
Sbjct: 103 EIQTMTGNKQVLVRKLDLADTKSIRAFAKGFLAEEKHLHILINNAGVMMCPYTKTVDGFE 162
Query: 572 THLGVNHLGTGVTTY 616
H+GVNHLG + T+
Sbjct: 163 MHMGVNHLGHFLLTH 177
Score = 132 bits (332), Expect = 1e-30
Identities = 68/120 (56%), Positives = 87/120 (72%), Gaps = 1/120 (0%)
Frame = +2
Query: 521 NAGVMLCPYSKTADG-FETHLGVNHLGTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFSR 697
N+G+ C +SK A+ F L G+G+T Y+VHPG V+SELVRHS + +W LFS
Sbjct: 216 NSGLAYC-HSKLANILFTQELARRLKGSGITAYSVHPGTVKSELVRHSPFMKWMWWLFSF 274
Query: 698 FLKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
F+KT ++GAQTSL+CA+ EGLE LSG +FSDC AWVS +ARN A RLW+VSC+LLGI
Sbjct: 275 FIKTPQQGAQTSLYCAITEGLEVLSGHHFSDCSVAWVSAQARNETIARRLWDVSCDLLGI 334
>ref|XP_854127.1| PREDICTED: similar to Retinol dehydrogenase 13 [Canis familiaris]
Length = 334
Score = 152 bits (383), Expect = 1e-36
Identities = 73/129 (56%), Positives = 92/129 (71%)
Frame = +2
Query: 212 IRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAAS 391
+R + AGG C + +PGK V++TGANTGIGK+TA ELARRG + +ACRD+ K E+AA
Sbjct: 21 LRDYIAGGACPSKATIPGKTVIVTGANTGIGKQTALELARRGGNIILACRDMEKCEAAAK 80
Query: 392 EIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFE 571
EIR +T N +V LDL+ KSIR FA + EE+Q+HIL+NNA VM CP+ T DGFE
Sbjct: 81 EIRGETLNHRVSAWHLDLASLKSIREFAAKIIEEEEQVHILVNNAAVMRCPHWTTKDGFE 140
Query: 572 THLGVNHLG 598
GVNHLG
Sbjct: 141 MQFGVNHLG 149
Score = 72.4 bits (176), Expect = 1e-12
Identities = 40/101 (39%), Positives = 59/101 (58%), Gaps = 6/101 (5%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHS------FLLCLLWRLFSRFLKTAREGAQTSLHCALAEG 757
GTGVT A+HPG+ ++EL RH+ F L +F +K+ + AQ S + A+AE
Sbjct: 220 GTGVTVNALHPGVARTELGRHTGMHSSAFSSFTLGPIFWLLVKSPQLAAQPSTYLAVAEE 279
Query: 758 LEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGIQ 880
LE +SGKYF K +P A + + A+RLW S L+G++
Sbjct: 280 LEGVSGKYFDGLKEKAPAPEAEDEEVAQRLWAESARLVGLE 320
>ref|XP_857582.1| PREDICTED: similar to retinol dehydrogenase 14 (all-trans and
9-cis) isoform 4 [Canis familiaris]
Length = 307
Score = 125 bits (313), Expect = 2e-28
Identities = 95/307 (30%), Positives = 147/307 (47%), Gaps = 60/307 (19%)
Frame = +2
Query: 137 AVETMLAVLGLLTSFLFFLY--VTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKE 310
AV + A+L L+ L+ P +++ GG + + GK V+ITGAN+G+G+
Sbjct: 2 AVAAVAALLAALSGVLWLAARRFVGPGVQQLHGGG---DSGLMHGKTVLITGANSGLGRA 58
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNS-------------QVLVRKLDLSD 451
TA L R GARV + CRD + E AA ++R + + + +++VR+LDL+
Sbjct: 59 TAAALLRLGARVIMGCRDRARAEEAAGQLRRELRQAGGREPGSDVGAAGELVVRELDLAS 118
Query: 452 TKSIRAFAEG-----------FLTEEKQLHILINNAGVMLCP------------------ 544
+S+RAF + FL L +L N+A +
Sbjct: 119 LRSVRAFCQEVLQFGVNHLGHFLLTNLLLGLLKNSAPSRIVVVSSKLYKYGDINFEDLNS 178
Query: 545 ---------YSKTADG---FETHLGVNHLGTGVTTYAVHPGIVQSELVRHSFLLCLLWRL 688
YS++ F L GT VT +HPGIV++ L RH + L+ L
Sbjct: 179 EQSYNKSFCYSRSKLANILFTRELARRLEGTNVTVNVLHPGIVRTNLGRHIHIPLLVRPL 238
Query: 689 FS----RFLKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNV 856
F+ F KT EGAQTS++ A + +E +SGKYF DCK + P+A + A +LW++
Sbjct: 239 FNLVSWAFFKTPVEGAQTSVYLASSPEVEGVSGKYFGDCKEEELLPKAMDESVARKLWDI 298
Query: 857 SCELLGI 877
S ++GI
Sbjct: 299 SEVMVGI 305
>ref|XP_540096.2| PREDICTED: similar to retinol dehydrogenase 14 (all-trans and
9-cis) isoform 1 [Canis familiaris]
Length = 336
Score = 122 bits (307), Expect = 8e-28
Identities = 68/169 (40%), Positives = 99/169 (58%), Gaps = 15/169 (8%)
Frame = +2
Query: 137 AVETMLAVLGLLTSFLFFLY--VTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKE 310
AV + A+L L+ L+ P +++ GG + + GK V+ITGAN+G+G+
Sbjct: 2 AVAAVAALLAALSGVLWLAARRFVGPGVQQLHGGG---DSGLMHGKTVLITGANSGLGRA 58
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNS-------------QVLVRKLDLSD 451
TA L R GARV + CRD + E AA ++R + + + +++VR+LDL+
Sbjct: 59 TAAALLRLGARVIMGCRDRARAEEAAGQLRRELRQAGGREPGSDVGAAGELVVRELDLAS 118
Query: 452 TKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLG 598
+S+RAF + L EE +L +LINNAG+ CPY KT DGFE GVNHLG
Sbjct: 119 LRSVRAFCQEVLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLG 167
Score = 79.3 bits (194), Expect = 1e-14
Identities = 42/98 (42%), Positives = 61/98 (62%), Gaps = 4/98 (4%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFS----RFLKTAREGAQTSLHCALAEGLE 763
GT VT +HPGIV++ L RH + L+ LF+ F KT EGAQTS++ A + +E
Sbjct: 237 GTNVTVNVLHPGIVRTNLGRHIHIPLLVRPLFNLVSWAFFKTPVEGAQTSVYLASSPEVE 296
Query: 764 PLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
+SGKYF DCK + P+A + A +LW++S ++GI
Sbjct: 297 GVSGKYFGDCKEEELLPKAMDESVARKLWDISEVMVGI 334
>ref|XP_548293.2| PREDICTED: similar to retinol dehydrogenase 11 (predicted) [Canis
familiaris]
Length = 377
Score = 116 bits (290), Expect = 8e-26
Identities = 71/166 (42%), Positives = 99/166 (59%), Gaps = 3/166 (1%)
Frame = +2
Query: 140 VETMLAVLGLLTSFLFFLY---VTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKE 310
+E +L GLL +Y V AP GG+ L G+ V+TGAN+GIGK
Sbjct: 1 MEALLLGAGLLLGAYVLIYYNLVKAPP-----CGGIA----SLRGRTAVVTGANSGIGKM 51
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLT 490
TA ELARRGARV +ACR +GE+AA ++R ++ N++V+ LDL+ S+RAFA FL+
Sbjct: 52 TALELARRGARVVLACRSRERGEAAAFDLRQESGNNEVIFMALDLASLASVRAFATAFLS 111
Query: 491 EEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTYAVHP 628
E +L ILI+NAG+ C +T F L VNH+G + T+ + P
Sbjct: 112 SEPRLDILIHNAGISSC--GRTHKPFNLLLRVNHIGPFLLTHLLLP 155
Score = 90.5 bits (223), Expect = 5e-18
Identities = 52/107 (48%), Positives = 65/107 (60%), Gaps = 3/107 (2%)
Frame = +2
Query: 566 FETHLGVNHLGTGVTTYAVHPGIVQSEL-VRH--SFLLCLLWRLFSRFLKTAREGAQTSL 736
F L GTGVT YA HPG V SEL +RH +L LL L L+ R GAQT L
Sbjct: 207 FARELATQLEGTGVTCYAAHPGPVNSELFLRHVPGWLCPLLRPLAWLMLRAPRGGAQTPL 266
Query: 737 HCALAEGLEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
+CAL EG+EPLSG+YF++C V P AR+++ A +LW S L G+
Sbjct: 267 YCALQEGIEPLSGRYFANCHVEEVPPAARDDRAAHQLWEASKRLAGL 313
>ref|XP_533000.2| PREDICTED: similar to Retinol dehydrogenase 12 [Canis familiaris]
Length = 596
Score = 103 bits (256), Expect = 7e-22
Identities = 53/125 (42%), Positives = 81/125 (64%)
Frame = +2
Query: 239 CRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNS 418
C T+ L GK V+TGAN+GIGK +ELARRGARV +ACR+ +G+ A +EI+ +K +
Sbjct: 309 CSTD--LTGKTAVVTGANSGIGKAVCQELARRGARVILACRNWERGQKALAEIQVASKGT 366
Query: 419 QVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLG 598
+L+ ++DLS SIR+FA L E ++H+L+NNA + P + T +G + N++G
Sbjct: 367 CLLLGQVDLSSMASIRSFARWLLQEYPEIHLLVNNAAISGFPKTLTPEGLDLTFATNYVG 426
Query: 599 TGVTT 613
+ T
Sbjct: 427 PFLLT 431
Score = 65.1 bits (157), Expect = 2e-10
Identities = 37/96 (38%), Positives = 60/96 (62%), Gaps = 2/96 (2%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVR-HSFLLCLLWRLFSRFLKTAREGAQTSLHCALAEGLEPLS 772
GTGVT +V PG+V +E+++ + +L L+ LFS F K ++GA L+ +LA+ L+ +S
Sbjct: 497 GTGVTVNSVDPGVVYTEIMKPYPWLYRFLFWLFSFFCKDVKQGAIPVLYLSLAKELDGVS 556
Query: 773 GKYF-SDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
GKYF S C + A++ + A+ LWN S +L +
Sbjct: 557 GKYFSSSCMITLPTEAAQDPQVAQSLWNASVQLTNL 592
>ref|XP_852623.1| PREDICTED: similar to WW domain-containing oxidoreductase isoform 1
[Canis familiaris]
Length = 383
Score = 103 bits (256), Expect = 7e-22
Identities = 55/137 (40%), Positives = 79/137 (57%), Gaps = 4/137 (2%)
Frame = +2
Query: 200 TAPSIRKFFAGGVCRTNM----QLPGKVVVITGANTGIGKETARELARRGARVYIACRDV 367
T P+ R+ + GG + GKVVV+TGAN+GIG ETA+ A GA V +ACR++
Sbjct: 99 TKPTSRQRYDGGTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNM 158
Query: 368 LKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPY 547
+ A S+I + ++V LDL+ +S++ FA+ F + LH+L+ NA P+
Sbjct: 159 TRANEAVSQILGEWHKAKVEAMTLDLALLRSVQHFAQAFKAKNVSLHVLVCNAAAFALPW 218
Query: 548 SKTADGFETHLGVNHLG 598
S T DG ET VNHLG
Sbjct: 219 SLTKDGLETTFQVNHLG 235
>ref|XP_852222.1| PREDICTED: similar to dehydrogenase/reductase (SDR family) X-linked
[Canis familiaris]
Length = 387
Score = 92.0 bits (227), Expect = 2e-18
Identities = 46/113 (40%), Positives = 66/113 (58%)
Frame = +2
Query: 260 PGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKL 439
P +V ++TG GIG TA+ LAR G V +A + +I+ +T N +V
Sbjct: 94 PERVAIVTGGTDGIGYSTAKHLARLGMHVILAGNNDSSAPDVVRKIQEETLNDKVEFLYC 153
Query: 440 DLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLG 598
DL+ +SIR F + F ++ LH+L+NNAGVM+ P T DGFE H G+N+LG
Sbjct: 154 DLASLRSIRQFVQKFKKKKIPLHVLVNNAGVMMVPERTTEDGFEEHFGLNYLG 206
Score = 51.6 bits (122), Expect = 2e-06
Identities = 35/96 (36%), Positives = 48/96 (50%), Gaps = 2/96 (2%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHSFL-LCLLWRLFSR-FLKTAREGAQTSLHCALAEGLEPL 769
G+ VT V PG+V + L RH F L+ +LF F KT EGA TS++ A+ LE L
Sbjct: 282 GSPVTANVVDPGVVNTNLYRHVFWGTRLIKKLFGWWFFKTPDEGAWTSVYAAVTPDLEGL 341
Query: 770 SGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
G+Y + K + LW SC++ GI
Sbjct: 342 GGRYLYNEKETKSLAVTYDLDLQTELWARSCQMTGI 377
>ref|XP_857503.1| PREDICTED: similar to retinol dehydrogenase 14 (all-trans and
9-cis) isoform 3 [Canis familiaris]
Length = 153
Score = 72.4 bits (176), Expect = 1e-12
Identities = 45/136 (33%), Positives = 73/136 (53%), Gaps = 15/136 (11%)
Frame = +2
Query: 137 AVETMLAVLGLLTSFLFFLY--VTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKE 310
AV + A+L L+ L+ P +++ GG + + GK V+ITGAN+G+G+
Sbjct: 2 AVAAVAALLAALSGVLWLAARRFVGPGVQQLHGGG---DSGLMHGKTVLITGANSGLGRA 58
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNS-------------QVLVRKLDLSD 451
TA L R GARV + CRD + E AA ++R + + + +++VR+LDL+
Sbjct: 59 TAAALLRLGARVIMGCRDRARAEEAAGQLRRELRQAGGREPGSDVGAAGELVVRELDLAS 118
Query: 452 TKSIRAFAEGFLTEEK 499
+S+RAF + L K
Sbjct: 119 LRSVRAFCQEVLQGRK 134
Database: RefSeqCP
Posted date: Aug 1, 2006 9:25 PM
Number of letters in database: 19,266,565
Number of sequences in database: 33,732
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,117,398
Number of Sequences: 33732
Number of extensions: 2115375
Number of successful extensions: 8420
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 6396
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8362
length of database: 19,266,565
effective HSP length: 108
effective length of database: 15,623,509
effective search space used: 7139943613
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqMP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-000326
(1697 letters)
Database: RefSeqMP
45,328 sequences; 21,768,885 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_084293.1| retinol dehydrogenase 12 [Mus musculus] 293 3e-79
Alignment gi|NP_067532.2| short-chain dehydrogenase/reductase 1 [Mus musc... 219 9e-57
Alignment gi|NP_780581.1| retinol dehydrogenase 13 (all-trans and 9-cis) ... 142 1e-33
Alignment gi|NP_076186.1| alcohol dehydrogenase PAN2 [Mus musculus] 123 7e-28
Alignment gi|NP_899109.1| hypothetical protein LOC70451 [Mus musculus] 117 5e-26
Alignment gi|NP_001028498.1| dehydrogenase/reductase (SDR family) X chrom... 109 8e-24
Alignment gi|NP_062519.2| WW-domain oxidoreductase [Mus musculus] 100 4e-21
Alignment gi|NP_079798.1| retinal short-chain dehydrogenase/reductase 4 [... 58 3e-08
Alignment gi|XP_487442.1| PREDICTED: similar to Carbonyl reductase [NADPH... 58 3e-08
Alignment gi|XP_989090.1| PREDICTED: similar to Carbonyl reductase [NADPH... 58 3e-08
>ref|NP_084293.1| retinol dehydrogenase 12 [Mus musculus]
Length = 316
Score = 293 bits (751), Expect = 3e-79
Identities = 172/317 (54%), Positives = 193/317 (60%), Gaps = 71/317 (22%)
Frame = +2
Query: 149 MLAVLGLLTSFLFFLYVTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELA 328
ML +L LLTSFL LY+TAPSIRKFFAGGVC TN+Q+PGKVVVITGANTGIGKETARELA
Sbjct: 1 MLFILVLLTSFLSILYLTAPSIRKFFAGGVCTTNVQIPGKVVVITGANTGIGKETARELA 60
Query: 329 RRGARVYIACRDVLKGESAASEIRA---------------DTKNSQVLVRKLDLSDTKSI 463
RRGARVYIACRDVLKGESAASEIRA DTK+ + + L++ K +
Sbjct: 61 RRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAERF-LAEEKKL 119
Query: 464 RAFAEGF---------LTEEKQLHILINNAGVMLCPY----------------------- 547
T+ + H +N+ G L Y
Sbjct: 120 HILINNAGVMMCPYSKTTDGFETHFGVNHLGHFLLTYLLLERLKESAPARVVNLSSIAHL 179
Query: 548 -----------------------SKTADG-FETHLGVNHLGTGVTTYAVHPGIVQSELVR 655
SK A+ F L GTGVT YAVHPG+V SE+ R
Sbjct: 180 IGKIRFHDLQGQKRYCSAFAYGHSKLANLLFTRELAKRLQGTGVTAYAVHPGVVLSEITR 239
Query: 656 HSFLLCLLWRLFSRFLKTAREGAQTSLHCALAEGLEPLSGKYFSDCKRAWVSPRARNNKT 835
+S+LLCLLWRLFS F K+ +GAQTSLHCALAE LEPLSGKYFSDCKR WVS RARN KT
Sbjct: 240 NSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPLSGKYFSDCKRMWVSSRARNKKT 299
Query: 836 AERLWNVSCELLGIQWE 886
AERLWNVSCELLGIQWE
Sbjct: 300 AERLWNVSCELLGIQWE 316
>ref|NP_067532.2| short-chain dehydrogenase/reductase 1 [Mus musculus]
Length = 316
Score = 219 bits (557), Expect = 9e-57
Identities = 107/150 (71%), Positives = 125/150 (83%)
Frame = +2
Query: 167 LLTSFLFFLYVTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARV 346
LL S F LY+ P IRK + GVC +N+QLPGKV ++TGANTGIGKETA++LA+RGARV
Sbjct: 6 LLLSLPFILYLVTPKIRKMLSSGVCTSNVQLPGKVAIVTGANTGIGKETAKDLAQRGARV 65
Query: 347 YIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNA 526
Y+ACRDV KGE AA EI+A T NSQV VRKLDL+DTKSIRAFA+ FL EEK LH+LINNA
Sbjct: 66 YLACRDVDKGELAAREIQAVTGNSQVFVRKLDLADTKSIRAFAKDFLAEEKHLHLLINNA 125
Query: 527 GVMLCPYSKTADGFETHLGVNHLGTGVTTY 616
GVM+CPYSKTADGFE H+GVNHLG + T+
Sbjct: 126 GVMMCPYSKTADGFEMHIGVNHLGHFLLTH 155
>ref|NP_780581.1| retinol dehydrogenase 13 (all-trans and 9-cis) [Mus musculus]
Length = 334
Score = 142 bits (358), Expect = 1e-33
Identities = 68/129 (52%), Positives = 91/129 (70%)
Frame = +2
Query: 212 IRKFFAGGVCRTNMQLPGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAAS 391
++ + AGG C + +PGK V++TGANTGIGK+TA ELA+RG V +ACRD+ K E AA
Sbjct: 21 LKDYVAGGACPSKATIPGKTVIVTGANTGIGKQTALELAKRGGNVILACRDMEKCEVAAK 80
Query: 392 EIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFE 571
+IR +T N +V +LDL+ KSIR FA + EE+++ IL+NNA VM CP+ T DGFE
Sbjct: 81 DIRGETLNPRVRAERLDLASLKSIREFARKVIKEEERVDILVNNAAVMRCPHWTTEDGFE 140
Query: 572 THLGVNHLG 598
GVN+LG
Sbjct: 141 MQFGVNYLG 149
Score = 69.3 bits (168), Expect = 1e-11
Identities = 39/100 (39%), Positives = 56/100 (56%), Gaps = 6/100 (6%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHS------FLLCLLWRLFSRFLKTAREGAQTSLHCALAEG 757
G+GVT A+HPG+ ++EL RH+ F +L F K+ + AQ S + A+AE
Sbjct: 220 GSGVTVNALHPGVARTELGRHTGMHNSAFSGFMLGPFFWLLFKSPQLAAQPSTYLAVAEE 279
Query: 758 LEPLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
LE +SGKYF + SP A + + A RLW S L+G+
Sbjct: 280 LENVSGKYFDGLREKAPSPEAEDEEVARRLWTESARLVGL 319
>ref|NP_076186.1| alcohol dehydrogenase PAN2 [Mus musculus]
Length = 334
Score = 123 bits (308), Expect = 7e-28
Identities = 62/122 (50%), Positives = 82/122 (67%), Gaps = 10/122 (8%)
Frame = +2
Query: 263 GKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRA----------DTK 412
GK V+ITGAN+G+G+ TA EL R GARV + CRD + E AA ++R D
Sbjct: 44 GKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRQELCQAGGAGPDGT 103
Query: 413 NSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNH 592
+ Q++V++LDL+ +S+RAF + L EE +L +LINNAGV CPY+KT DGFE GVNH
Sbjct: 104 DGQLVVKELDLASLRSVRAFCQELLQEEPRLDVLINNAGVFHCPYTKTEDGFEMQFGVNH 163
Query: 593 LG 598
LG
Sbjct: 164 LG 165
Score = 79.0 bits (193), Expect = 1e-14
Identities = 41/98 (41%), Positives = 60/98 (61%), Gaps = 4/98 (4%)
Frame = +2
Query: 596 GTGVTTYAVHPGIVQSELVRHSFLLCLLWRLFS----RFLKTAREGAQTSLHCALAEGLE 763
GT VT +HPGIV++ L RH + L LF+ F KT EGAQTS++ A + +E
Sbjct: 235 GTNVTVNVLHPGIVRTNLGRHIHIPLLARPLFNLVSWAFFKTPLEGAQTSIYLACSPDVE 294
Query: 764 PLSGKYFSDCKRAWVSPRARNNKTAERLWNVSCELLGI 877
+SG+YF DCK + P+A + A +LW++S ++GI
Sbjct: 295 GVSGRYFGDCKEEELLPKAMDESVARKLWDISEVMVGI 332
>ref|NP_899109.1| hypothetical protein LOC70451 [Mus musculus]
Length = 260
Score = 117 bits (292), Expect = 5e-26
Identities = 71/166 (42%), Positives = 102/166 (61%), Gaps = 3/166 (1%)
Frame = +2
Query: 140 VETMLAVLGLLTSFLFFLY---VTAPSIRKFFAGGVCRTNMQLPGKVVVITGANTGIGKE 310
+E +L GLL +Y V APS GG+ L G+ VV+TGAN+GIGK
Sbjct: 1 MEMLLLGAGLLLGAYVLVYYNLVKAPS-----CGGIG----SLRGRTVVVTGANSGIGKM 51
Query: 311 TARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLT 490
TA ELARRGARV +ACR +GE+AA ++R ++ N++V+ LDL+ S++AFA FL+
Sbjct: 52 TALELARRGARVVLACRSRERGEAAAFDLRQESGNNEVIFMALDLASLASVQAFATAFLS 111
Query: 491 EEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTYAVHP 628
E +L +LI+NAG+ C +T + F L VNH+G + T+ + P
Sbjct: 112 SEPRLDVLIHNAGISSC--GRTRETFNLLLRVNHVGPFLLTHLLLP 155
>ref|NP_001028498.1| dehydrogenase/reductase (SDR family) X chromosome [Mus musculus]
Length = 335
Score = 109 bits (273), Expect = 8e-24
Identities = 57/125 (45%), Positives = 77/125 (61%)
Frame = +2
Query: 260 PGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKL 439
PG+V ++TGA GIG+ TAR+LAR G V +A D +G+ S IRA+ + + L
Sbjct: 42 PGRVAIVTGATAGIGRSTARQLARLGMCVVVAGNDEHRGQEVVSSIRAEMGSDRAHFLPL 101
Query: 440 DLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPYSKTADGFETHLGVNHLGTGVTTYA 619
DL+ S+R FA F LH+L+NNAGVML P ++T DGFE HLGVN LG + T
Sbjct: 102 DLASLASVRGFARDFQALGLPLHLLVNNAGVMLEPRAETEDGFERHLGVNFLGHFLLTLL 161
Query: 620 VHPGI 634
+ P +
Sbjct: 162 LLPAL 166
>ref|NP_062519.2| WW-domain oxidoreductase [Mus musculus]
Length = 414
Score = 100 bits (250), Expect = 4e-21
Identities = 54/137 (39%), Positives = 77/137 (56%), Gaps = 4/137 (2%)
Frame = +2
Query: 200 TAPSIRKFFAGGVCRTNM----QLPGKVVVITGANTGIGKETARELARRGARVYIACRDV 367
T P+ R+ + G + GKVV++TGAN+GIG ETA+ A GA V +ACR++
Sbjct: 99 TKPTTRQRYDGSTTAMEILQGRDFTGKVVLVTGANSGIGFETAKSFALHGAHVILACRNL 158
Query: 368 LKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAEGFLTEEKQLHILINNAGVMLCPY 547
+ A S I + ++V LDL+ +S++ FAE F + LH+L+ NAG P+
Sbjct: 159 SRASEAVSRILEEWHKAKVEAMTLDLAVLRSVQHFAEAFKAKNVSLHVLVCNAGTFALPW 218
Query: 548 SKTADGFETHLGVNHLG 598
T DG ET VNHLG
Sbjct: 219 GLTKDGLETTFQVNHLG 235
Score = 71.2 bits (173), Expect = 3e-12
Identities = 38/91 (41%), Positives = 60/91 (65%), Gaps = 1/91 (1%)
Frame = +2
Query: 602 GVTTYAVHPG-IVQSELVRHSFLLCLLWRLFSRFLKTAREGAQTSLHCALAEGLEPLSGK 778
GVT+ AVHPG ++ S + R+S++ LL+ L F K+ ++GA T+++CA+A LE L G
Sbjct: 315 GVTSNAVHPGNMMYSAIHRNSWVYKLLFTLARPFTKSMQQGAATTVYCAVAPELEGLGGM 374
Query: 779 YFSDCKRAWVSPRARNNKTAERLWNVSCELL 871
YF++C R S A++ +TA LW +S L+
Sbjct: 375 YFNNCCRCLPSEEAQSEETARALWELSERLI 405
>ref|NP_079798.1| retinal short-chain dehydrogenase/reductase 4 [Mus musculus]
Length = 288
Score = 58.2 bits (139), Expect = 3e-08
Identities = 39/128 (30%), Positives = 65/128 (50%), Gaps = 4/128 (3%)
Frame = +2
Query: 269 VVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEI--RADTKNSQVLVRKLD 442
VV +TGA++GIG+E A +L++ G + ++ R + E + K +LV LD
Sbjct: 2 VVWVTGASSGIGEELAFQLSKLGVSLVLSARRAQELERVKRRCLENGNLKEKDILVLPLD 61
Query: 443 LSDTKSIRAFAEGFLTEEKQLHILINNAG--VMLCPYSKTADGFETHLGVNHLGTGVTTY 616
L+DT S A + L E ++ IL+NN G D F+ + +N++GT T
Sbjct: 62 LTDTSSHEAATKAVLQEFGKIDILVNNGGRSQRSLVLETNLDVFKELINLNYIGTVSLTK 121
Query: 617 AVHPGIVQ 640
V P +++
Sbjct: 122 CVLPHMIE 129
>ref|XP_487442.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) [Mus musculus]
Length = 277
Score = 57.8 bits (138), Expect = 3e-08
Identities = 32/90 (35%), Positives = 53/90 (58%), Gaps = 1/90 (1%)
Frame = +2
Query: 266 KVVVITGANTGIGKETARELARR-GARVYIACRDVLKGESAASEIRADTKNSQVLVRKLD 442
+V ++TG N GIG E R+L ++ V + RD +G +A +++A+ N + +LD
Sbjct: 6 RVALVTGGNKGIGFEIIRDLCQKFSGDVVLTARDEARGCAAVQKLQAEGLNPRF--HQLD 63
Query: 443 LSDTKSIRAFAEGFLTEEKQLHILINNAGV 532
+ D +SI A + L E K L +L+NNAG+
Sbjct: 64 IDDPQSIHALRDFLLKEYKGLDVLVNNAGI 93
>ref|XP_989090.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) [Mus musculus]
Length = 277
Score = 57.8 bits (138), Expect = 3e-08
Identities = 32/90 (35%), Positives = 53/90 (58%), Gaps = 1/90 (1%)
Frame = +2
Query: 266 KVVVITGANTGIGKETARELARR-GARVYIACRDVLKGESAASEIRADTKNSQVLVRKLD 442
+V ++TG N GIG E R+L ++ V + RD +G +A +++A+ N + +LD
Sbjct: 6 RVALVTGGNKGIGFEIIRDLCQKFSGDVVLTARDEARGCAAVQKLQAEGLNPRF--HQLD 63
Query: 443 LSDTKSIRAFAEGFLTEEKQLHILINNAGV 532
+ D +SI A + L E K L +L+NNAG+
Sbjct: 64 IDDPQSIHALRDFLLKEYKGLDVLVNNAGI 93
Database: RefSeqMP
Posted date: Aug 2, 2006 12:58 AM
Number of letters in database: 21,768,885
Number of sequences in database: 45,328
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,569,716
Number of Sequences: 45328
Number of extensions: 2391200
Number of successful extensions: 9162
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 7029
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 9056
length of database: 21,768,885
effective HSP length: 109
effective length of database: 16,828,133
effective search space used: 7673628648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)