BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006193
(1065 letters)
Database: RefSeqSP
1040 sequences; 434,620 total letters
Searching...done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_999191.1| cAMP-dependent protein kinase type I regulatory... 530 e-152
Alignment gi|NP_999423.1| cAMP-dependent protein kinase regulatory subuni... 79 1e-16
>ref|NP_999191.1| cAMP-dependent protein kinase type I regulatory subunit [Sus scrofa]
Length = 380
Score = 530 bits (1365), Expect(2) = e-152
Identities = 272/288 (94%), Positives = 272/288 (94%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXXXXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEE 345
MASGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEE
Sbjct: 1 MASGSTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEE 60
Query: 346 AKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKD 525
AKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKD
Sbjct: 61 AKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKD 120
Query: 526 YKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGE 705
YKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGE
Sbjct: 121 YKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGE 180
Query: 706 MDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLR 885
MDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWG DRDSYRRILMGSTLR
Sbjct: 181 MDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGNDRDSYRRILMGSTLR 240
Query: 886 KRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
KRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 241 KRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 288
Score = 70.5 bits (171), Expect = 7e-14
Identities = 54/174 (31%), Positives = 84/174 (48%), Gaps = 12/174 (6%)
Frame = +1
Query: 430 VVKGRRRRGAISAEVYTE---EDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNER 600
++ G R + A+ + D SY R ++ + + + K + LD ER
Sbjct: 204 LIYGTPRAATVKAKTNVKLWGNDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWER 263
Query: 601 SDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVY---VNNEWATSVGEGGS---F 762
+ DA+ PV F G+ ++ QG+ GD F++I +G V NE VG G F
Sbjct: 264 LTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYF 323
Query: 763 GELALIYGTPRAATVKAKTNVKLWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
GE+AL+ PRAATV A+ +K +DR + R+L S + KR + Y F+S
Sbjct: 324 GEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 377
Score = 23.9 bits (50), Expect(2) = e-152
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 287 PGDEFFIILEGSAAV 301
>ref|NP_999423.1| cAMP-dependent protein kinase regulatory subunit [Sus scrofa]
Length = 155
Score = 79.3 bits (194), Expect = 1e-16
Identities = 56/144 (38%), Positives = 81/144 (56%), Gaps = 7/144 (4%)
Frame = +1
Query: 373 ASARADSREDE-ISPPPPNPVVKGRRRRGAISAEVYT--EEDAASYVRKVIPKDYKTMAA 543
A A ++S +DE + P P+ RR ++ AE Y EE+ + R + PK +
Sbjct: 16 AIADSESEDDEDLDVPIPSRF----DRRVSVCAETYNPDEEEEDTDPRVIHPKTDQQRCR 71
Query: 544 LAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVN 723
L +A + +LF +LD + S + DAMF + E VI Q D+GDNFYVI++G D+ V
Sbjct: 72 LQEACKDILLFKNLDQEQLSQVLDAMFERTVKVDEHVIDQRDDGDNFYVIERGTYDILVT 131
Query: 724 NEWAT-SVGE---GGSFGELALIY 783
+ T SVG+ GSFGELAL+Y
Sbjct: 132 KDNQTRSVGQYDNHGSFGELALMY 155
Database: RefSeqSP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 434,620
Number of sequences in database: 1040
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 998,284
Number of Sequences: 1040
Number of extensions: 24948
Number of successful extensions: 91
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 79
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 434,620
effective HSP length: 78
effective length of database: 353,500
effective search space used: 97566000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqBP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006193
(1065 letters)
Database: RefSeqBP
33,508 sequences; 16,112,626 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_871872.1| PREDICTED: similar to cAMP-dependent protein ki... 459 e-129
Alignment gi|XP_581646.2| PREDICTED: similar to protein kinase, cAMP-depe... 369 e-102
Alignment gi|XP_874825.1| PREDICTED: similar to cAMP-dependent protein ki... 165 6e-41
Alignment gi|NP_777074.1| cAMP-dependent protein kinase, regulatory subun... 154 1e-37
Alignment gi|NP_776861.1| protein kinase, cGMP-dependent, type I [Bos tau... 120 2e-27
Alignment gi|XP_612707.2| PREDICTED: similar to protein kinase, cGMP-depe... 104 1e-22
Alignment gi|XP_596590.2| PREDICTED: similar to protein kinase, cAMP-depe... 82 9e-16
Alignment gi|XP_887653.1| PREDICTED: similar to RAP guanine-nucleotide-ex... 54 2e-07
Alignment gi|XP_887647.1| PREDICTED: similar to RAP guanine-nucleotide-ex... 54 2e-07
Alignment gi|XP_615454.2| PREDICTED: similar to RAP guanine-nucleotide-ex... 54 2e-07
>ref|XP_871872.1| PREDICTED: similar to cAMP-dependent protein kinase type I-alpha
regulatory subunit (Tissue-specific extinguisher-1)
(TSE1) [Bos taurus]
Length = 363
Score = 459 bits (1180), Expect(2) = e-129
Identities = 242/288 (84%), Positives = 247/288 (85%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXXXXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEE 345
MASG+TA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFE+LEKEE
Sbjct: 1 MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEE 60
Query: 346 AKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKD 525
AKQIQNLQKA +RADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRK
Sbjct: 61 AKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKA---- 116
Query: 526 YKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGE 705
+ + AL N SDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGE
Sbjct: 117 -ENLEALRLT------------NRLSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGE 163
Query: 706 MDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLR 885
MDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLR
Sbjct: 164 MDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLR 223
Query: 886 KRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
KRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 224 KRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 271
Score = 69.7 bits (169), Expect = 4e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 209 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 268
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 269 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 328
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 329 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 360
Score = 23.9 bits (50), Expect(2) = e-129
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 270 PGDEFFIILEGSAAV 284
>ref|XP_581646.2| PREDICTED: similar to protein kinase, cAMP-dependent, regulatory,
type I, beta [Bos taurus]
Length = 377
Score = 369 bits (947), Expect = e-102
Identities = 194/269 (72%), Positives = 213/269 (79%)
Frame = +1
Query: 223 YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARADSRED 402
YVQ+ IQ +LKD IVQLC A+PERPM FLRE+FERLEK ++ Q S R
Sbjct: 21 YVQRRGIQQVLKDCIVQLCLAKPERPMRFLREHFERLEKASVREPVRRQTCSQVDCVR-- 78
Query: 403 EISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSH 582
+ P + RR A V + D A Y++ VIPKDYK M ALAKAI KNVLFSH
Sbjct: 79 -LCATPQTAAHQAPPRRDCGVAPV-CQPDVAHYLKSVIPKDYKNMTALAKAISKNVLFSH 136
Query: 583 LDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSF 762
LDDNERSDIFDAMFPV+ IAGETVIQQGDEGDNFYVIDQGE+DVYVN EW TS+ EGGSF
Sbjct: 137 LDDNERSDIFDAMFPVTHIAGETVIQQGDEGDNFYVIDQGEVDVYVNGEWVTSISEGGSF 196
Query: 763 GELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLD 942
GELALIYGTPRAATVKAKT++KLWGIDRDSYRRILMGSTLRKR+MYEEFLSKVSIL SL+
Sbjct: 197 GELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRRMYEEFLSKVSILASLE 256
Query: 943 KWERLTVADALEPVQFEDGQKIVVQETRG 1029
KWERLTVADALEPVQFEDG+KIVVQ G
Sbjct: 257 KWERLTVADALEPVQFEDGEKIVVQGEPG 285
Score = 70.1 bits (170), Expect = 3e-12
Identities = 50/152 (32%), Positives = 78/152 (51%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + + L+ ER + DA+ PV F GE ++ QG
Sbjct: 223 DRDSYRRILMGSTLRKRRMYEEFLSKVSILASLEKWERLTVADALEPVQFEDGEKIVVQG 282
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD+F++I +G V +E VG G FGE+AL+ PRAATV A+ +K
Sbjct: 283 EPGDDFFIITEGTASVLQRRSPSEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLK 342
Query: 829 LWGIDRDSYRRIL-MGSTLRKRKM--YEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 343 CVKLDRPRFERVLGPCSEILKRNIQRYNSFIS 374
>ref|XP_874825.1| PREDICTED: similar to cAMP-dependent protein kinase type I-alpha
regulatory subunit [Bos taurus]
Length = 130
Score = 165 bits (417), Expect = 6e-41
Identities = 83/116 (71%), Positives = 100/116 (86%)
Frame = +1
Query: 433 VKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIF 612
++G ++GAI AEVYT++DA SYVRKV P+ YKTM ALAKA+EKNVL +HLDD+ERS+ F
Sbjct: 1 MRGWGQQGAIYAEVYTKQDATSYVRKVGPEGYKTMTALAKAVEKNVLSAHLDDSERSNHF 60
Query: 613 DAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALI 780
DA+FPVSFIAGE VIQQG E +NF+VIDQGEM+V VN+EWATSVGEG S GE+A I
Sbjct: 61 DAVFPVSFIAGEIVIQQGRERNNFFVIDQGEMEVCVNSEWATSVGEGRSVGEIASI 116
>ref|NP_777074.1| cAMP-dependent protein kinase, regulatory subunit beta 2 [Bos taurus]
Length = 418
Score = 154 bits (389), Expect = 1e-37
Identities = 87/205 (42%), Positives = 121/205 (59%), Gaps = 8/205 (3%)
Frame = +1
Query: 427 PVVKGRRRRGAISAEVYT----EEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDN 594
PV+ RR ++ AE Y E+DA S R + PK L +A + +LF +LD
Sbjct: 104 PVINRFTRRASVCAEAYNPDEEEDDAES--RIIHPKTDDQRNRLQEACKDILLFKNLDPE 161
Query: 595 ERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVN----NEWATSVGEGGSF 762
+ S + DAMF GE VI QGD+GDNFYVID+G D+YV + GSF
Sbjct: 162 QMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSF 221
Query: 763 GELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLD 942
GELAL+Y TPRAAT+ A + LWG+DR ++RRI++ + +KRKMYE F+ + L+SL+
Sbjct: 222 GELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLE 281
Query: 943 KWERLTVADALEPVQFEDGQKIVVQ 1017
ERL V D + + DG++I+ Q
Sbjct: 282 VSERLKVVDVIGTKVYNDGEQIIAQ 306
Score = 65.5 bits (158), Expect = 7e-11
Identities = 44/156 (28%), Positives = 72/156 (46%), Gaps = 14/156 (8%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L+ +ER + D + + GE +I QG
Sbjct: 248 DRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQG 307
Query: 667 DEGDNFYVIDQGEMDVYVNNEWATSVGEGGS-----------FGELALIYGTPRAATVKA 813
D D+F++++ GE+ + + + + V E G+ FGELAL+ PRAA+ A
Sbjct: 308 DSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHA 367
Query: 814 KTNVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFL 912
VK +D ++ R+L M R YEE L
Sbjct: 368 IGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQL 403
>ref|NP_776861.1| protein kinase, cGMP-dependent, type I [Bos taurus]
Length = 671
Score = 120 bits (301), Expect = 2e-27
Identities = 83/267 (31%), Positives = 134/267 (50%), Gaps = 3/267 (1%)
Frame = +1
Query: 253 LKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARADSREDEISPPPPNPV 432
L++ ++ + ER ++E +RL ++E ++IQ L++ + S + P P +
Sbjct: 4 LEEDFAKILMLKEER----IKELEKRLSEKE-EEIQELKRKLHKCQS----VLPVPSTHI 54
Query: 433 VKGRRRRGAISAEVYTEE---DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERS 603
R ISAE T D RK K ++ + +AI N +L+ ++
Sbjct: 55 GPRTTRAQGISAEPQTYRSFHDLRQAFRK-FTKSERSKDLIKEAILDNDFMKNLELSQIQ 113
Query: 604 DIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIY 783
+I D M+PV + +I++GD G YV++ G+++V ++G G FGELA++Y
Sbjct: 114 EIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILY 173
Query: 784 GTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTV 963
R ATVK NVKLW IDR ++ I+M + L K Y EFL V +SL + +
Sbjct: 174 NCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKL 233
Query: 964 ADALEPVQFEDGQKIVVQETRGRVLHI 1044
AD LE +E+G+ I+ Q RG I
Sbjct: 234 ADVLEETHYENGEYIIRQGARGDTFFI 260
Score = 55.1 bits (131), Expect = 9e-08
Identities = 37/121 (30%), Positives = 60/121 (49%), Gaps = 6/121 (4%)
Frame = +1
Query: 574 FSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNE------WA 735
F L + S + D + + GE +I+QG GD F++I +G+++V + +
Sbjct: 222 FQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPNEDPVFL 281
Query: 736 TSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLS 915
++G+G FGE AL R A V A V IDRDS++ ++ G K YE+ +
Sbjct: 282 RTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEA 341
Query: 916 K 918
K
Sbjct: 342 K 342
>ref|XP_612707.2| PREDICTED: similar to protein kinase, cGMP-dependent, type II [Bos
taurus]
Length = 792
Score = 104 bits (259), Expect = 1e-22
Identities = 60/208 (28%), Positives = 108/208 (51%), Gaps = 9/208 (4%)
Frame = +1
Query: 448 RRGA---ISAEVYT------EEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNER 600
RRGA +SAE T + S+ + + KD + A+ KN LD +
Sbjct: 148 RRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQI 207
Query: 601 SDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALI 780
D+ + M+ ++ G +I+QG+ G++ +V+ +G ++V + +S+ +FGELA++
Sbjct: 208 KDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVSQGEKLLSSIPMWTTFGELAIL 267
Query: 781 YGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLT 960
Y R A+VKA TNVK W +DR+ ++ I+ + + + Y FL VS+L++L + +
Sbjct: 268 YNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTK 327
Query: 961 VADALEPVQFEDGQKIVVQETRGRVLHI 1044
+ D LE ++ G I+ + G I
Sbjct: 328 IIDCLEVEYYDKGDYIIREGEEGSTFFI 355
>ref|XP_596590.2| PREDICTED: similar to protein kinase, cAMP-dependent, regulatory,
type 2, alpha [Bos taurus]
Length = 272
Score = 81.6 bits (200), Expect = 9e-16
Identities = 54/152 (35%), Positives = 76/152 (50%), Gaps = 4/152 (2%)
Frame = +1
Query: 601 SDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWAT-SVGE---GGSFGE 768
S + DAMF + E VI QGD+GDNFYVI++G D+ V + T SVG+ GSFGE
Sbjct: 51 SQVLDAMFERTVKVDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNHGSFGE 110
Query: 769 LALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKW 948
LAL+Y TPRAAT+ A + LWG+ VS
Sbjct: 111 LALMYNTPRAATIVATSEGSLWGL--------------------------VS-------- 136
Query: 949 ERLTVADALEPVQFEDGQKIVVQETRGRVLHI 1044
ER+ + D + ++DG++I+ Q + +I
Sbjct: 137 ERMKIVDVIGEKVYKDGERIITQGEKADSFYI 168
Score = 59.3 bits (142), Expect = 5e-09
Identities = 41/127 (32%), Positives = 64/127 (50%), Gaps = 17/127 (13%)
Frame = +1
Query: 592 NERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGS---- 759
+ER I D + + GE +I QG++ D+FY+I+ GE+ + + ++ T V + G
Sbjct: 136 SERMKIVDVIGEKVYKDGERIITQGEKADSFYIIESGEVSILIKSK--TKVNKDGENQEV 193
Query: 760 ----------FGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRIL---MGSTLRKRKMY 900
FGELAL+ PRAA+ A +VK +D ++ R+L M R Y
Sbjct: 194 EIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHY 253
Query: 901 EEFLSKV 921
EE L K+
Sbjct: 254 EEQLVKM 260
>ref|XP_887653.1| PREDICTED: similar to RAP guanine-nucleotide-exchange factor 3
isoform 5 [Bos taurus]
Length = 883
Score = 54.3 bits (129), Expect = 2e-07
Identities = 35/122 (28%), Positives = 66/122 (54%), Gaps = 3/122 (2%)
Frame = +1
Query: 577 SHLDDNERSDIFDAM-FPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEW-ATSVGE 750
+HL ++ + ++ + F AG + QGD+G ++Y+I +G ++V + + T++ E
Sbjct: 247 AHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHE 306
Query: 751 GGSFGELALIYGTPRAAT-VKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSI 927
G FG+LAL+ PRAAT + + N +D+ + RI+ + ++ EE V +
Sbjct: 307 GDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRL-EEHGKVVLV 365
Query: 928 LE 933
LE
Sbjct: 366 LE 367
>ref|XP_887647.1| PREDICTED: similar to RAP guanine-nucleotide-exchange factor 3
isoform 4 [Bos taurus]
Length = 924
Score = 54.3 bits (129), Expect = 2e-07
Identities = 35/122 (28%), Positives = 66/122 (54%), Gaps = 3/122 (2%)
Frame = +1
Query: 577 SHLDDNERSDIFDAM-FPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEW-ATSVGE 750
+HL ++ + ++ + F AG + QGD+G ++Y+I +G ++V + + T++ E
Sbjct: 247 AHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHE 306
Query: 751 GGSFGELALIYGTPRAAT-VKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSI 927
G FG+LAL+ PRAAT + + N +D+ + RI+ + ++ EE V +
Sbjct: 307 GDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRL-EEHGKVVLV 365
Query: 928 LE 933
LE
Sbjct: 366 LE 367
>ref|XP_615454.2| PREDICTED: similar to RAP guanine-nucleotide-exchange factor 3
isoform 1 [Bos taurus]
Length = 923
Score = 54.3 bits (129), Expect = 2e-07
Identities = 35/122 (28%), Positives = 66/122 (54%), Gaps = 3/122 (2%)
Frame = +1
Query: 577 SHLDDNERSDIFDAM-FPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEW-ATSVGE 750
+HL ++ + ++ + F AG + QGD+G ++Y+I +G ++V + + T++ E
Sbjct: 247 AHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHE 306
Query: 751 GGSFGELALIYGTPRAAT-VKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSI 927
G FG+LAL+ PRAAT + + N +D+ + RI+ + ++ EE V +
Sbjct: 307 GDDFGQLALVNDAPRAATIILGEDNCHFLRVDKQDFNRIIKDVEAKTMRL-EEHGKVVLV 365
Query: 928 LE 933
LE
Sbjct: 366 LE 367
Database: RefSeqBP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 16,112,626
Number of sequences in database: 33,508
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,346,532
Number of Sequences: 33508
Number of extensions: 952166
Number of successful extensions: 4238
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 3481
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4201
length of database: 16,112,626
effective HSP length: 102
effective length of database: 12,694,810
effective search space used: 3199092120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqCP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006193
(1065 letters)
Database: RefSeqCP
33,732 sequences; 19,266,565 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_862649.1| PREDICTED: similar to cAMP-dependent protein ki... 528 e-150
Alignment gi|XP_537577.2| PREDICTED: similar to cAMP-dependent protein ki... 528 e-150
Alignment gi|XP_862625.1| PREDICTED: similar to cAMP-dependent protein ki... 501 e-142
Alignment gi|XP_862606.1| PREDICTED: similar to protein kinase, cAMP depe... 498 e-141
Alignment gi|XP_537920.2| PREDICTED: similar to protein kinase, cAMP-depe... 447 e-126
Alignment gi|XP_856004.1| PREDICTED: similar to protein kinase, cAMP-depe... 435 e-122
Alignment gi|XP_857982.1| PREDICTED: similar to cAMP-dependent protein ki... 162 7e-40
Alignment gi|XP_858066.1| PREDICTED: similar to cAMP-dependent protein ki... 157 2e-38
Alignment gi|XP_857939.1| PREDICTED: similar to cAMP-dependent protein ki... 157 2e-38
Alignment gi|XP_857899.1| PREDICTED: similar to cAMP-dependent protein ki... 157 2e-38
>ref|XP_862649.1| PREDICTED: similar to cAMP-dependent protein kinase type I-alpha
regulatory subunit (Tissue-specific extinguisher-1)
(TSE1) isoform 4 [Canis familiaris]
Length = 381
Score = 528 bits (1359), Expect(2) = e-150
Identities = 272/289 (94%), Positives = 273/289 (94%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
MASGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE
Sbjct: 1 MASGSTATSEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 60
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
EAKQIQNLQKAS+RADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 61 EAKQIQNLQKASSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 120
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 121 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 180
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 181 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 240
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 241 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 289
Score = 69.7 bits (169), Expect = 5e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVY---VNNEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 347 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 378
Score = 23.9 bits (50), Expect(2) = e-150
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 288 PGDEFFIILEGSAAV 302
>ref|XP_537577.2| PREDICTED: similar to cAMP-dependent protein kinase type I-alpha
regulatory subunit (Tissue-specific extinguisher-1)
(TSE1) isoform 1 [Canis familiaris]
Length = 381
Score = 528 bits (1359), Expect(2) = e-150
Identities = 272/289 (94%), Positives = 273/289 (94%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
MASGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE
Sbjct: 1 MASGSTATSEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 60
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
EAKQIQNLQKAS+RADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 61 EAKQIQNLQKASSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 120
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 121 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 180
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 181 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 240
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 241 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 289
Score = 69.7 bits (169), Expect = 5e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVY---VNNEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 347 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 378
Score = 23.9 bits (50), Expect(2) = e-150
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 288 PGDEFFIILEGSAAV 302
>ref|XP_862625.1| PREDICTED: similar to cAMP-dependent protein kinase type I-alpha
regulatory subunit isoform 3 [Canis familiaris]
Length = 377
Score = 501 bits (1289), Expect(2) = e-142
Identities = 261/289 (90%), Positives = 265/289 (91%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
MASGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEK
Sbjct: 1 MASGSTATSEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKV 60
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
+ +Q N ++RADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 61 KIRQGGN----NSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 116
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 117 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 176
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 177 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 236
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 237 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 285
Score = 69.7 bits (169), Expect = 5e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 223 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 282
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 283 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 342
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 343 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 374
Score = 23.9 bits (50), Expect(2) = e-142
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 284 PGDEFFIILEGSAAV 298
>ref|XP_862606.1| PREDICTED: similar to protein kinase, cAMP dependent regulatory, type
I, alpha isoform 2 [Canis familiaris]
Length = 373
Score = 498 bits (1282), Expect(2) = e-141
Identities = 260/289 (89%), Positives = 263/289 (91%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
MASGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEK
Sbjct: 1 MASGSTATSEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEK- 59
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
++ S+RADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 60 -------VKITSSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 112
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 113 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 172
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 173 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 232
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 233 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 281
Score = 69.7 bits (169), Expect = 5e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 219 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 278
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 279 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 338
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 339 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 370
Score = 23.9 bits (50), Expect(2) = e-141
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 280 PGDEFFIILEGSAAV 294
>ref|XP_537920.2| PREDICTED: similar to protein kinase, cAMP-dependent, regulatory,
type I, beta isoform 1 [Canis familiaris]
Length = 377
Score = 447 bits (1150), Expect = e-126
Identities = 221/269 (82%), Positives = 243/269 (90%)
Frame = +1
Query: 223 YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARADSRED 402
YVQKH +Q +LKD IV LC ++P+RPM FLRE+FE+LEKEE +QIQ+ QK+ DS ++
Sbjct: 21 YVQKHGVQQVLKDCIVHLCISKPDRPMTFLREHFEKLEKEENRQIQSQQKS----DSHDE 76
Query: 403 EISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSH 582
E+SP PPNPVVK RRRRG +SAEVYTEEDA SYVRKVIPKDYKTM ALAKAI KNVLF+H
Sbjct: 77 EVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAH 136
Query: 583 LDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSF 762
LDDNERSDIFDAMFPV+ IAGETVIQQGDEGDNFYVIDQGE+DVYVN EW TS+ EGGSF
Sbjct: 137 LDDNERSDIFDAMFPVTHIAGETVIQQGDEGDNFYVIDQGEVDVYVNGEWVTSISEGGSF 196
Query: 763 GELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLD 942
GELALIYGTPRAATVKAKT++KLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESL+
Sbjct: 197 GELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLE 256
Query: 943 KWERLTVADALEPVQFEDGQKIVVQETRG 1029
KWERLTVADALEPVQFEDG+KIVVQ G
Sbjct: 257 KWERLTVADALEPVQFEDGEKIVVQGEPG 285
Score = 71.2 bits (173), Expect = 2e-12
Identities = 51/152 (33%), Positives = 77/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + L+ ER + DA+ PV F GE ++ QG
Sbjct: 223 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQG 282
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD+F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 283 EPGDDFFIITEGTASVLQRRSPNEEYMEVGRLGPSDYFGEIALLLNRPRAATVVARGPLK 342
Query: 829 LWGIDRDSYRRIL-MGSTLRKRKM--YEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 343 CVKLDRPRFERVLGPCSEILKRNIQRYNSFIS 374
>ref|XP_856004.1| PREDICTED: similar to protein kinase, cAMP-dependent, regulatory,
type I, beta isoform 3 [Canis familiaris]
Length = 380
Score = 435 bits (1118), Expect = e-122
Identities = 218/274 (79%), Positives = 238/274 (86%), Gaps = 5/274 (1%)
Frame = +1
Query: 223 YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARA----- 387
YVQKH +Q +LKD IV LC ++P+RPM FLRE+FE+LEK NLQ R+
Sbjct: 21 YVQKHGVQQVLKDCIVHLCISKPDRPMTFLREHFEKLEK------MNLQTGDGRSAVPPS 74
Query: 388 DSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKN 567
DS ++E+SP PPNPVVK RRRRG +SAEVYTEEDA SYVRKVIPKDYKTM ALAKAI KN
Sbjct: 75 DSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKN 134
Query: 568 VLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVG 747
VLF+HLDDNERSDIFDAMFPV+ IAGETVIQQGDEGDNFYVIDQGE+DVYVN EW TS+
Sbjct: 135 VLFAHLDDNERSDIFDAMFPVTHIAGETVIQQGDEGDNFYVIDQGEVDVYVNGEWVTSIS 194
Query: 748 EGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSI 927
EGGSFGELALIYGTPRAATVKAKT++KLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSI
Sbjct: 195 EGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSI 254
Query: 928 LESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
LESL+KWERLTVADALEPVQFEDG+KIVVQ G
Sbjct: 255 LESLEKWERLTVADALEPVQFEDGEKIVVQGEPG 288
Score = 71.2 bits (173), Expect = 2e-12
Identities = 51/152 (33%), Positives = 77/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + L+ ER + DA+ PV F GE ++ QG
Sbjct: 226 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQG 285
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD+F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 286 EPGDDFFIITEGTASVLQRRSPNEEYMEVGRLGPSDYFGEIALLLNRPRAATVVARGPLK 345
Query: 829 LWGIDRDSYRRIL-MGSTLRKRKM--YEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 346 CVKLDRPRFERVLGPCSEILKRNIQRYNSFIS 377
>ref|XP_857982.1| PREDICTED: similar to cAMP-dependent protein kinase type II-beta
regulatory subunit isoform 9 [Canis familiaris]
Length = 398
Score = 162 bits (409), Expect = 7e-40
Identities = 98/279 (35%), Positives = 150/279 (53%), Gaps = 23/279 (8%)
Frame = +1
Query: 250 LLKDSIVQLCTARPERPMAFLREYFERLEKEEAK---------------QIQNLQKASAR 384
LL+ V++ +P + F ++F RL++E + ++ ++ A
Sbjct: 12 LLQGFTVEVLRHQPADLLDFALQHFTRLQQENERRGAAIISPPNRRPFQEVFGVKTAQEE 71
Query: 385 ADSREDEISPPPPNPVVKGRRRRGAISAEVYT----EEDAASYVRKVIPKDYKTMAALAK 552
+++ + P PV+ RR ++ AE Y E+DA S R + PK L +
Sbjct: 72 GEAKLNCSDRDPKTPVINRFTRRASVCAEAYNPDEEEDDAES--RIIHPKTDDQRNRLQE 129
Query: 553 AIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVN--- 723
A + +LF +LD + S + DAMF GE VI QGD+GDNFYVID+G D+YV
Sbjct: 130 ACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDG 189
Query: 724 -NEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMY 900
+ GSFGELAL+Y TPRAAT+ A + LWG+DR ++RRI++ + +KRKMY
Sbjct: 190 VGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMY 249
Query: 901 EEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ 1017
E F+ + L+SL+ ERL V D + + DG++I+ Q
Sbjct: 250 ESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQ 288
Score = 62.0 bits (149), Expect = 9e-10
Identities = 45/154 (29%), Positives = 70/154 (45%), Gaps = 12/154 (7%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L+ +ER + D + + GE +I QG
Sbjct: 230 DRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQG 289
Query: 667 DEGDNFYVIDQGEM------DVYVNNEWATSV---GEGGSFGELALIYGTPRAATVKAKT 819
D D+F++++ GE+ V V + A + G FGELAL+ PRAA+ A
Sbjct: 290 DSADSFFIVESGEVKITMKRKVKVEEDGAVEIARCSRGQYFGELALVTNKPRAASAHAIG 349
Query: 820 NVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFL 912
VK +D ++ R+L M R YEE L
Sbjct: 350 TVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQL 383
>ref|XP_858066.1| PREDICTED: similar to cAMP-dependent protein kinase, regulatory
subunit beta 2 isoform 10 [Canis familiaris]
Length = 412
Score = 157 bits (397), Expect = 2e-38
Identities = 93/228 (40%), Positives = 130/228 (57%), Gaps = 9/228 (3%)
Frame = +1
Query: 361 NLQKASARADSRE-DEISPPPPNPVVKGRRRRGAISAEVYT----EEDAASYVRKVIPKD 525
N + AR DS + +E PV+ RR ++ AE Y E+DA S R + PK
Sbjct: 75 NFAEEPARTDSEDAEEEEEEYVAPVINRFTRRASVCAEAYNPDEEEDDAES--RIIHPKT 132
Query: 526 YKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGE 705
L +A + +LF +LD + S + DAMF GE VI QGD+GDNFYVID+G
Sbjct: 133 DDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGT 192
Query: 706 MDVYVN----NEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMG 873
D+YV + GSFGELAL+Y TPRAAT+ A + LWG+DR ++RRI++
Sbjct: 193 FDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVK 252
Query: 874 STLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ 1017
+ +KRKMYE F+ + L+SL+ ERL V D + + DG++I+ Q
Sbjct: 253 NNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQ 300
Score = 65.1 bits (157), Expect = 1e-10
Identities = 44/156 (28%), Positives = 72/156 (46%), Gaps = 14/156 (8%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L+ +ER + D + + GE +I QG
Sbjct: 242 DRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQG 301
Query: 667 DEGDNFYVIDQGEMDVYVNNEWATSVGEGGS-----------FGELALIYGTPRAATVKA 813
D D+F++++ GE+ + + + + V E G+ FGELAL+ PRAA+ A
Sbjct: 302 DSADSFFIVESGEVKITMKRKGKSEVEEDGAVEIARCSRGQYFGELALVTNKPRAASAHA 361
Query: 814 KTNVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFL 912
VK +D ++ R+L M R YEE L
Sbjct: 362 IGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQL 397
>ref|XP_857939.1| PREDICTED: similar to cAMP-dependent protein kinase type II-beta
regulatory subunit isoform 8 [Canis familiaris]
Length = 402
Score = 157 bits (396), Expect = 2e-38
Identities = 101/293 (34%), Positives = 147/293 (50%), Gaps = 37/293 (12%)
Frame = +1
Query: 250 LLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARADSREDEISPPPP-- 423
LL+ V++ +P + F ++F RL++E + R + D +PP P
Sbjct: 12 LLQGFTVEVLRHQPADLLDFALQHFTRLQQENER----------RGPASPDPAAPPQPAA 61
Query: 424 ---------------------------NPVVKGRRRRGAISAEVYT----EEDAASYVRK 510
PV+ RR ++ AE Y E+DA S R
Sbjct: 62 RRPPPTPQPTERAQAGGEAEGEGDSLLTPVINRFTRRASVCAEAYNPDEEEDDAES--RI 119
Query: 511 VIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYV 690
+ PK L +A + +LF +LD + S + DAMF GE VI QGD+GDNFYV
Sbjct: 120 IHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYV 179
Query: 691 IDQGEMDVYVN----NEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYR 858
ID+G D+YV + GSFGELAL+Y TPRAAT+ A + LWG+DR ++R
Sbjct: 180 IDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFR 239
Query: 859 RILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ 1017
RI++ + +KRKMYE F+ + L+SL+ ERL V D + + DG++I+ Q
Sbjct: 240 RIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQ 292
Score = 62.0 bits (149), Expect = 9e-10
Identities = 45/154 (29%), Positives = 70/154 (45%), Gaps = 12/154 (7%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L+ +ER + D + + GE +I QG
Sbjct: 234 DRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQG 293
Query: 667 DEGDNFYVIDQGEM------DVYVNNEWATSV---GEGGSFGELALIYGTPRAATVKAKT 819
D D+F++++ GE+ V V + A + G FGELAL+ PRAA+ A
Sbjct: 294 DSADSFFIVESGEVKITMKRKVKVEEDGAVEIARCSRGQYFGELALVTNKPRAASAHAIG 353
Query: 820 NVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFL 912
VK +D ++ R+L M R YEE L
Sbjct: 354 TVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQL 387
>ref|XP_857899.1| PREDICTED: similar to cAMP-dependent protein kinase type II-beta
regulatory subunit isoform 7 [Canis familiaris]
Length = 398
Score = 157 bits (396), Expect = 2e-38
Identities = 100/286 (34%), Positives = 152/286 (53%), Gaps = 30/286 (10%)
Frame = +1
Query: 250 LLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQ----------------------IQN 363
LL+ V++ +P + F ++F RL++E ++
Sbjct: 12 LLQGFTVEVLRHQPADLLDFALQHFTRLQQENERRGAPASAPPGPGKTFEFPNEKLICDP 71
Query: 364 LQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYT----EEDAASYVRKVIPKDYK 531
L+K+S + ++ ++ PP V+ RR ++ AE Y E+DA S R + PK
Sbjct: 72 LRKSS---NEKQQQLEPP----VINRFTRRASVCAEAYNPDEEEDDAES--RIIHPKTDD 122
Query: 532 TMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMD 711
L +A + +LF +LD + S + DAMF GE VI QGD+GDNFYVID+G D
Sbjct: 123 QRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFD 182
Query: 712 VYVN----NEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGST 879
+YV + GSFGELAL+Y TPRAAT+ A + LWG+DR ++RRI++ +
Sbjct: 183 IYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNN 242
Query: 880 LRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ 1017
+KRKMYE F+ + L+SL+ ERL V D + + DG++I+ Q
Sbjct: 243 AKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQ 288
Score = 62.0 bits (149), Expect = 9e-10
Identities = 45/154 (29%), Positives = 70/154 (45%), Gaps = 12/154 (7%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L+ +ER + D + + GE +I QG
Sbjct: 230 DRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQG 289
Query: 667 DEGDNFYVIDQGEM------DVYVNNEWATSV---GEGGSFGELALIYGTPRAATVKAKT 819
D D+F++++ GE+ V V + A + G FGELAL+ PRAA+ A
Sbjct: 290 DSADSFFIVESGEVKITMKRKVKVEEDGAVEIARCSRGQYFGELALVTNKPRAASAHAIG 349
Query: 820 NVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFL 912
VK +D ++ R+L M R YEE L
Sbjct: 350 TVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQL 383
Database: RefSeqCP
Posted date: Aug 1, 2006 9:25 PM
Number of letters in database: 19,266,565
Number of sequences in database: 33,732
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,914,548
Number of Sequences: 33732
Number of extensions: 1122416
Number of successful extensions: 5180
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 4284
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5097
length of database: 19,266,565
effective HSP length: 104
effective length of database: 15,758,437
effective search space used: 3939609250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqHP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006193
(1065 letters)
Database: RefSeqHP
39,411 sequences; 17,774,539 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_997637.1| cAMP-dependent protein kinase, regulatory subun... 517 e-147
Alignment gi|NP_002725.1| cAMP-dependent protein kinase, regulatory subun... 517 e-147
Alignment gi|NP_997636.1| cAMP-dependent protein kinase, regulatory subun... 517 e-147
Alignment gi|NP_002726.1| protein kinase, cAMP-dependent, regulatory, typ... 446 e-125
Alignment gi|NP_004148.1| cAMP-dependent protein kinase, regulatory subun... 158 8e-39
Alignment gi|NP_002727.2| cAMP-dependent protein kinase, regulatory subun... 156 3e-38
Alignment gi|NP_006249.1| protein kinase, cGMP-dependent, type I [Homo sa... 120 2e-27
Alignment gi|XP_933702.1| PREDICTED: similar to cAMP-dependent protein ki... 107 1e-23
Alignment gi|NP_006250.1| protein kinase, cGMP-dependent, type II [Homo s... 106 3e-23
Alignment gi|XP_933699.1| PREDICTED: similar to cAMP-dependent protein ki... 99 8e-21
>ref|NP_997637.1| cAMP-dependent protein kinase, regulatory subunit alpha 1 [Homo
sapiens]
Length = 381
Score = 517 bits (1332), Expect(2) = e-147
Identities = 267/289 (92%), Positives = 267/289 (92%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
M SGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE
Sbjct: 1 MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 60
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
EAKQIQNLQKA R DSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 61 EAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 120
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMF VSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 121 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQG 180
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
E DVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 181 ETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 240
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 241 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 289
Score = 69.7 bits (169), Expect = 4e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVY---VNNEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 347 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 378
Score = 23.9 bits (50), Expect(2) = e-147
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 288 PGDEFFIILEGSAAV 302
>ref|NP_002725.1| cAMP-dependent protein kinase, regulatory subunit alpha 1 [Homo
sapiens]
Length = 381
Score = 517 bits (1332), Expect(2) = e-147
Identities = 267/289 (92%), Positives = 267/289 (92%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
M SGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE
Sbjct: 1 MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 60
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
EAKQIQNLQKA R DSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 61 EAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 120
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMF VSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 121 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQG 180
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
E DVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 181 ETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 240
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 241 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 289
Score = 69.7 bits (169), Expect = 4e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVY---VNNEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 347 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 378
Score = 23.9 bits (50), Expect(2) = e-147
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 288 PGDEFFIILEGSAAV 302
>ref|NP_997636.1| cAMP-dependent protein kinase, regulatory subunit alpha 1 [Homo
sapiens]
Length = 381
Score = 517 bits (1332), Expect(2) = e-147
Identities = 267/289 (92%), Positives = 267/289 (92%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
M SGSTA YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE
Sbjct: 1 MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 60
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
EAKQIQNLQKA R DSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 61 EAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 120
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMF VSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 121 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQG 180
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
E DVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 181 ETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 240
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 241 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 289
Score = 69.7 bits (169), Expect = 4e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVY---VNNEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 347 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 378
Score = 23.9 bits (50), Expect(2) = e-147
Identities = 12/15 (80%), Positives = 12/15 (80%), Gaps = 1/15 (6%)
Frame = +2
Query: 1022 PGDEFF-IFRGSAAV 1063
PGDEFF I GSAAV
Sbjct: 288 PGDEFFIILEGSAAV 302
>ref|NP_002726.1| protein kinase, cAMP-dependent, regulatory, type I, beta [Homo
sapiens]
Length = 381
Score = 446 bits (1147), Expect = e-125
Identities = 219/274 (79%), Positives = 246/274 (89%)
Frame = +1
Query: 223 YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARADSRED 402
YVQ H IQ +LKD IV LC ++PERPM FLRE+FE+LEKEE +QI QK+++++DS ++
Sbjct: 21 YVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDE 80
Query: 403 EISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSH 582
E+SP PPNPVVK RRRRG +SAEVYTEEDA SYVRKVIPKDYKTM ALAKAI KNVLF+H
Sbjct: 81 EVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAH 140
Query: 583 LDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSF 762
LDDNERSDIFDAMFPV+ IAGETVIQQG+EGDNFYV+DQGE+DVYVN EW T++ EGGSF
Sbjct: 141 LDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSF 200
Query: 763 GELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLD 942
GELALIYGTPRAATVKAKT++KLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESL+
Sbjct: 201 GELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLE 260
Query: 943 KWERLTVADALEPVQFEDGQKIVVQETRGRVLHI 1044
KWERLTVADALEPVQFEDG+KIVVQ G +I
Sbjct: 261 KWERLTVADALEPVQFEDGEKIVVQGEPGDDFYI 294
Score = 73.2 bits (178), Expect = 4e-13
Identities = 52/152 (34%), Positives = 77/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + L+ ER + DA+ PV F GE ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD+FY+I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL-MGSTLRKRKM--YEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 347 CVKLDRPRFERVLGPCSEILKRNIQRYNSFIS 378
>ref|NP_004148.1| cAMP-dependent protein kinase, regulatory subunit alpha 2 [Homo
sapiens]
Length = 404
Score = 158 bits (399), Expect = 8e-39
Identities = 104/289 (35%), Positives = 157/289 (54%), Gaps = 24/289 (8%)
Frame = +1
Query: 250 LLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQI--------QNL----------QKA 375
LL+ V++ +P + F EYF RL + A Q+L + A
Sbjct: 13 LLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVA 72
Query: 376 SARADSREDEISPPPPNPVVKGRRRRGAISAEVYT--EEDAASYVRKVIPKDYKTMAALA 549
A+ DS +E PV RR ++ AE Y EE+ + R + PK + L
Sbjct: 73 DAKGDSESEE-DEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQ 131
Query: 550 KAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNE 729
+A + +LF +LD + S + DAMF A E VI QGD+GDNFYVI++G D+ V +
Sbjct: 132 EACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKD 191
Query: 730 WAT-SVGE---GGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKM 897
T SVG+ GSFGELAL+Y TPRAAT+ A + LWG+DR ++RRI++ + +KRKM
Sbjct: 192 NQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKM 251
Query: 898 YEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRGRVLHI 1044
+E F+ V +L+SL+ ER+ + D + ++DG++I+ Q + +I
Sbjct: 252 FESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYI 300
Score = 66.6 bits (161), Expect = 3e-11
Identities = 48/161 (29%), Positives = 77/161 (47%), Gaps = 16/161 (9%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L L+ +ER I D + + GE +I QG
Sbjct: 233 DRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQG 292
Query: 667 DEGDNFYVIDQGEMDVYVNNEWATSVGEGGS-------------FGELALIYGTPRAATV 807
++ D+FY+I+ GE+ + + + S +GG+ FGELAL+ PRAA+
Sbjct: 293 EKADSFYIIESGEVSILIRSR-TKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASA 351
Query: 808 KAKTNVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFLSKV 921
A +VK +D ++ R+L M R YEE L K+
Sbjct: 352 YAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKM 392
>ref|NP_002727.2| cAMP-dependent protein kinase, regulatory subunit beta 2 [Homo
sapiens]
Length = 418
Score = 156 bits (394), Expect = 3e-38
Identities = 91/222 (40%), Positives = 127/222 (57%), Gaps = 12/222 (5%)
Frame = +1
Query: 388 DSREDEISPPPPN----PVVKGRRRRGAISAEVYT----EEDAASYVRKVIPKDYKTMAA 543
D E+E +P PV+ RR ++ AE Y E+DA S R + PK
Sbjct: 87 DGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAES--RIIHPKTDDQRNR 144
Query: 544 LAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVN 723
L +A + +LF +LD + S + DAMF GE VI QGD+GDNFYVID+G D+YV
Sbjct: 145 LQEACKDILLFKNLDPEQMSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVK 204
Query: 724 ----NEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKR 891
+ GSFGELAL+Y TPRAAT+ A + LWG+DR ++RRI++ + +KR
Sbjct: 205 CDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKR 264
Query: 892 KMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ 1017
KMYE F+ + L+SL+ ERL V D + + DG++I+ Q
Sbjct: 265 KMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQ 306
Score = 65.5 bits (158), Expect = 7e-11
Identities = 44/156 (28%), Positives = 72/156 (46%), Gaps = 14/156 (8%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L+ +ER + D + + GE +I QG
Sbjct: 248 DRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQG 307
Query: 667 DEGDNFYVIDQGEMDVYVNNEWATSVGEGGS-----------FGELALIYGTPRAATVKA 813
D D+F++++ GE+ + + + + V E G+ FGELAL+ PRAA+ A
Sbjct: 308 DSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHA 367
Query: 814 KTNVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFL 912
VK +D ++ R+L M R YEE L
Sbjct: 368 IGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQL 403
>ref|NP_006249.1| protein kinase, cGMP-dependent, type I [Homo sapiens]
Length = 686
Score = 120 bits (302), Expect = 2e-27
Identities = 77/245 (31%), Positives = 123/245 (50%), Gaps = 2/245 (0%)
Frame = +1
Query: 316 EYFERLEKEEAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAE--VYTEED 489
E ++L+ E K ++ A+ +A + P R +R AISAE + +D
Sbjct: 37 ELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEP------RTKRQAISAEPTAFDIQD 90
Query: 490 AASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGD 669
+ PK ++ + +AI N +L+ ++ +I D M+PV + +I++GD
Sbjct: 91 LSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGD 150
Query: 670 EGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRD 849
G YV++ G+++V ++G G FGELA++Y R ATVK NVKLW IDR
Sbjct: 151 VGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQ 210
Query: 850 SYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
++ I+M + L K Y EFL V +SL + +AD LE +E+G+ I+ Q RG
Sbjct: 211 CFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARG 270
Query: 1030 RVLHI 1044
I
Sbjct: 271 DTFFI 275
Score = 54.3 bits (129), Expect = 2e-07
Identities = 37/121 (30%), Positives = 59/121 (48%), Gaps = 6/121 (4%)
Frame = +1
Query: 574 FSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNE------WA 735
F L + S + D + + GE +I+QG GD F++I +G ++V + +
Sbjct: 237 FQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFL 296
Query: 736 TSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLS 915
++G+G FGE AL R A V A V IDRDS++ ++ G K YE+ +
Sbjct: 297 RTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEA 356
Query: 916 K 918
K
Sbjct: 357 K 357
>ref|XP_933702.1| PREDICTED: similar to cAMP-dependent protein kinase type I-beta
regulatory subunit [Homo sapiens]
Length = 153
Score = 107 bits (268), Expect = 1e-23
Identities = 52/69 (75%), Positives = 60/69 (86%), Gaps = 2/69 (2%)
Frame = +1
Query: 712 VYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMG--STLR 885
VYVN EW T++ EGGSFGELALIYGTPRAATVKAKT++KLWGIDRDSYRRILMG S L+
Sbjct: 17 VYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGTASVLQ 76
Query: 886 KRKMYEEFL 912
+R EE++
Sbjct: 77 RRSPNEEYV 85
>ref|NP_006250.1| protein kinase, cGMP-dependent, type II [Homo sapiens]
Length = 762
Score = 106 bits (265), Expect = 3e-23
Identities = 73/275 (26%), Positives = 133/275 (48%), Gaps = 30/275 (10%)
Frame = +1
Query: 310 LREYFERLEKEE---AKQIQNLQKASARADSREDEI-----SPPPPNP------------ 429
L+E E+L K+ A+ + LQ + + +D + SP +P
Sbjct: 51 LKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTS 110
Query: 430 -VVKGRRRRGA---ISAEVYT------EEDAASYVRKVIPKDYKTMAALAKAIEKNVLFS 579
+V RRGA +SAE T + S+ + + KD + A+ KN
Sbjct: 111 GLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLK 170
Query: 580 HLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGS 759
LD + D+ + M+ ++ G +I+QG+ G++ +V+ +G ++V+ + +S+ +
Sbjct: 171 RLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVFQGEKLLSSIPMWTT 230
Query: 760 FGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESL 939
FGELA++Y R A+VKA TNVK W +DR+ ++ I+ + + + Y FL VS+L++L
Sbjct: 231 FGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNL 290
Query: 940 DKWERLTVADALEPVQFEDGQKIVVQETRGRVLHI 1044
+ + + D LE ++ G I+ + G I
Sbjct: 291 PEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFI 325
>ref|XP_933699.1| PREDICTED: similar to cAMP-dependent protein kinase type I-beta
regulatory subunit [Homo sapiens]
Length = 99
Score = 98.6 bits (244), Expect = 8e-21
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +1
Query: 859 RILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRGRVL 1038
R+ GSTLRKRKMYEEFLSKVSILESL+KWERLTVADALEPVQFEDG+KIVVQ G
Sbjct: 17 RVASGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDF 76
Query: 1039 HI 1044
+I
Sbjct: 77 YI 78
Database: RefSeqHP
Posted date: Aug 2, 2006 12:57 AM
Number of letters in database: 17,774,539
Number of sequences in database: 39,411
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,355,497
Number of Sequences: 39411
Number of extensions: 1110214
Number of successful extensions: 4850
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 3974
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4822
length of database: 17,774,539
effective HSP length: 103
effective length of database: 13,715,206
effective search space used: 3442516706
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqMP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006193
(1065 letters)
Database: RefSeqMP
45,328 sequences; 21,768,885 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_068680.1| protein kinase, cAMP dependent regulatory, type... 514 e-146
Alignment gi|NP_032949.2| protein kinase, cAMP dependent regulatory, type... 446 e-125
Alignment gi|NP_032950.1| protein kinase, cAMP dependent regulatory, type... 154 1e-37
Alignment gi|NP_035288.2| protein kinase, cAMP dependent regulatory, type... 154 2e-37
Alignment gi|NP_035290.1| protein kinase, cGMP-dependent, type I beta iso... 120 3e-27
Alignment gi|NP_001013855.1| protein kinase, cGMP-dependent, type I alpha... 119 4e-27
Alignment gi|NP_032952.2| protein kinase, cGMP-dependent, type II [Mus mu... 106 5e-23
Alignment gi|NP_659099.1| Rap1 guanine-nucleotide-exchange factor directl... 54 2e-07
Alignment gi|NP_062662.1| cAMP-regulated guanine nucleotide exchange fact... 53 6e-07
>ref|NP_068680.1| protein kinase, cAMP dependent regulatory, type I, alpha [Mus
musculus]
Length = 381
Score = 514 bits (1323), Expect = e-146
Identities = 265/289 (91%), Positives = 266/289 (92%), Gaps = 1/289 (0%)
Frame = +1
Query: 166 MASGSTAXXXXXXXXXX-XXYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKE 342
MASGS A YVQKHNIQALLKDSIVQLCT RPERPMAFLREYFERLEKE
Sbjct: 1 MASGSMATSEEERSLRECELYVQKHNIQALLKDSIVQLCTTRPERPMAFLREYFERLEKE 60
Query: 343 EAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 522
EA+QIQ LQK R DSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK
Sbjct: 61 EARQIQCLQKTGIRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPK 120
Query: 523 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 702
DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG
Sbjct: 121 DYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQG 180
Query: 703 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 882
EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL
Sbjct: 181 EMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTL 240
Query: 883 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQ G
Sbjct: 241 RKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPG 289
Score = 69.3 bits (168), Expect = 6e-12
Identities = 51/152 (33%), Positives = 76/152 (50%), Gaps = 9/152 (5%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + LD ER + DA+ PV F G+ ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVY---VNNEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD F++I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDEFFIILEGTAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL-MGSTLRKR--KMYEEFLS 915
+DR + R+L S + KR + Y F+S
Sbjct: 347 CVKLDRPRFERVLGPCSDILKRNIQQYNSFVS 378
>ref|NP_032949.2| protein kinase, cAMP dependent regulatory, type I beta [Mus musculus]
Length = 381
Score = 446 bits (1148), Expect = e-125
Identities = 220/274 (80%), Positives = 245/274 (89%)
Frame = +1
Query: 223 YVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARADSRED 402
YVQKH IQ +LK+ IV LC A+P+RP+ FLRE+FE+LEKEE +QI QK++++ DS ++
Sbjct: 21 YVQKHGIQQVLKECIVHLCVAKPDRPLRFLREHFEKLEKEENRQILARQKSNSQCDSHDE 80
Query: 403 EISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSH 582
EISP PPNPVVK RRRRG +SAEVYTEEDA SYVRKVIPKDYKTM ALAKAI KNVLFSH
Sbjct: 81 EISPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFSH 140
Query: 583 LDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSF 762
LDDNERSDIFDAMFPV+ I GETVIQQG+EGDNFYVIDQGE+DVYVN EW T++ EGGSF
Sbjct: 141 LDDNERSDIFDAMFPVTHIGGETVIQQGNEGDNFYVIDQGEVDVYVNGEWVTNISEGGSF 200
Query: 763 GELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLD 942
GELALIYGTPRAATVKAKT++KLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESL+
Sbjct: 201 GELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLE 260
Query: 943 KWERLTVADALEPVQFEDGQKIVVQETRGRVLHI 1044
KWERLTVADALEPVQFEDG+KIVVQ G +I
Sbjct: 261 KWERLTVADALEPVQFEDGEKIVVQGEPGDDFYI 294
Score = 72.0 bits (175), Expect = 1e-12
Identities = 46/133 (34%), Positives = 68/133 (51%), Gaps = 6/133 (4%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D SY R ++ + + + K + L+ ER + DA+ PV F GE ++ QG
Sbjct: 227 DRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQG 286
Query: 667 DEGDNFYVIDQGEMDVYVN---NEWATSVGEGGS---FGELALIYGTPRAATVKAKTNVK 828
+ GD+FY+I +G V NE VG G FGE+AL+ PRAATV A+ +K
Sbjct: 287 EPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLK 346
Query: 829 LWGIDRDSYRRIL 867
+DR + R+L
Sbjct: 347 CVKLDRPRFERVL 359
>ref|NP_032950.1| protein kinase, cAMP dependent regulatory, type II alpha [Mus
musculus]
Length = 402
Score = 154 bits (390), Expect = 1e-37
Identities = 99/290 (34%), Positives = 157/290 (54%), Gaps = 25/290 (8%)
Frame = +1
Query: 250 LLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNL---------QKASA------- 381
LL+ V++ +P + F EYF RL + ++ Q++SA
Sbjct: 13 LLQGYTVEVLRQQPPDLVDFAVEYFTRLREARRQESDTFIVSPTTFHTQESSAVPVIEED 72
Query: 382 ---RADSREDEISPPPPNPVVKGRRRRGAISAEVYT--EEDAASYVRKVIPKDYKTMAAL 546
+DS + ++ P P+ RR ++ AE + EE+ + R V PK + L
Sbjct: 73 GESDSDSEDADLEVPVPSKFT----RRVSVCAETFNPDEEEEDNDPRVVHPKTDEQRCRL 128
Query: 547 AKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNN 726
+A + +LF +LD + S + DAMF E VI QGD+GDNFYVI++G D+ V
Sbjct: 129 QEACKDILLFKNLDQEQLSQVLDAMFEKIVKTDEHVIDQGDDGDNFYVIERGTYDILVTK 188
Query: 727 EWAT-SVGE---GGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRK 894
+ T SVG+ GSFGELAL+Y TPRAAT+ A + LWG+DR ++RRI++ + +KRK
Sbjct: 189 DNQTRSVGQYDNRGSFGELALMYNTPRAATIIATSEGSLWGLDRVTFRRIIVKNNAKKRK 248
Query: 895 MYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRGRVLHI 1044
M+E F+ V + +SL+ ER+ + D + ++DG++I+ Q + +I
Sbjct: 249 MFESFIESVPLFKSLEMSERMKIVDVIGEKIYKDGERIIAQGEKADSFYI 298
Score = 69.7 bits (169), Expect = 5e-12
Identities = 47/160 (29%), Positives = 77/160 (48%), Gaps = 15/160 (9%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE LF L+ +ER I D + + GE +I QG
Sbjct: 231 DRVTFRRIIVKNNAKKRKMFESFIESVPLFKSLEMSERMKIVDVIGEKIYKDGERIIAQG 290
Query: 667 DEGDNFYVIDQGEMDVYVNNEWATSVGEGGS------------FGELALIYGTPRAATVK 810
++ D+FY+I+ GE+ + + ++ ++ G FGELAL+ PRAA+
Sbjct: 291 EKADSFYIIESGEVSILIRSKTKSNKNGGNQEVEIAHCHKGQYFGELALVTNKPRAASAY 350
Query: 811 AKTNVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFLSKV 921
A +VK +D ++ R+L M R YEE L K+
Sbjct: 351 AVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKM 390
>ref|NP_035288.2| protein kinase, cAMP dependent regulatory, type II beta [Mus
musculus]
Length = 416
Score = 154 bits (389), Expect = 2e-37
Identities = 87/205 (42%), Positives = 121/205 (59%), Gaps = 8/205 (3%)
Frame = +1
Query: 427 PVVKGRRRRGAISAEVYT----EEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDN 594
PV+ RR ++ AE Y E+DA S R + PK L +A + +LF +LD
Sbjct: 102 PVINRFTRRASVCAEAYNPDEEEDDAES--RIIHPKTDDQRNRLQEACKDILLFKNLDPE 159
Query: 595 ERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVN----NEWATSVGEGGSF 762
+ S + DAMF GE VI QGD+GDNFYVID+G D+YV + GSF
Sbjct: 160 QMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSF 219
Query: 763 GELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLD 942
GELAL+Y TPRAAT+ A + LWG+DR ++RRI++ + +KRKMYE F+ + L+SL+
Sbjct: 220 GELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLE 279
Query: 943 KWERLTVADALEPVQFEDGQKIVVQ 1017
ERL V D + + DG++I+ Q
Sbjct: 280 VSERLKVVDVIGTKVYNDGEQIIAQ 304
Score = 64.3 bits (155), Expect = 2e-10
Identities = 44/156 (28%), Positives = 72/156 (46%), Gaps = 14/156 (8%)
Frame = +1
Query: 487 DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQG 666
D ++ R ++ + K IE L+ +ER + D + + GE +I QG
Sbjct: 246 DRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQG 305
Query: 667 DEGDNFYVIDQGEMDVYVNNEWATSVGEGGS-----------FGELALIYGTPRAATVKA 813
D D+F++++ GE+ + + + + V E G+ FGELAL+ PRAA+ A
Sbjct: 306 DLADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCFRGQYFGELALVTNKPRAASAHA 365
Query: 814 KTNVKLWGIDRDSYRRIL---MGSTLRKRKMYEEFL 912
VK +D ++ R+L M R YEE L
Sbjct: 366 IGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQL 401
>ref|NP_035290.1| protein kinase, cGMP-dependent, type I beta isoform [Mus musculus]
Length = 686
Score = 120 bits (300), Expect = 3e-27
Identities = 77/245 (31%), Positives = 122/245 (49%), Gaps = 2/245 (0%)
Frame = +1
Query: 316 EYFERLEKEEAKQIQNLQKASARADSREDEISPPPPNPVVKGRRRRGAISAE--VYTEED 489
E ++L+ E K ++ A+ +A + P R +R AISAE + +D
Sbjct: 37 ELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEP------RTKRQAISAEPTAFDIQD 90
Query: 490 AASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGD 669
+ PK ++ + +AI N +L+ ++ +I D M+PV + +I++GD
Sbjct: 91 LSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGD 150
Query: 670 EGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRD 849
G YV++ G+++V ++G G FGELA++Y R ATVK NVKLW IDR
Sbjct: 151 VGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQ 210
Query: 850 SYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQETRG 1029
++ I+M + L K Y EFL V +SL +AD LE +E+G+ I+ Q RG
Sbjct: 211 CFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARG 270
Query: 1030 RVLHI 1044
I
Sbjct: 271 DTFFI 275
Score = 57.0 bits (136), Expect = 3e-08
Identities = 38/121 (31%), Positives = 60/121 (49%), Gaps = 6/121 (4%)
Frame = +1
Query: 574 FSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNE------WA 735
F L D S + D + + GE +I+QG GD F++I +G+++V + +
Sbjct: 237 FQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGQVNVTREDSPSEDPVFL 296
Query: 736 TSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLS 915
++G+G FGE AL R A V A V IDRDS++ ++ G K YE+ +
Sbjct: 297 RTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEA 356
Query: 916 K 918
K
Sbjct: 357 K 357
>ref|NP_001013855.1| protein kinase, cGMP-dependent, type I alpha isoform [Mus musculus]
Length = 671
Score = 119 bits (299), Expect = 4e-27
Identities = 83/267 (31%), Positives = 133/267 (49%), Gaps = 3/267 (1%)
Frame = +1
Query: 253 LKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKASARADSREDEISPPPPNPV 432
L++ ++ + ER ++E +RL ++E ++IQ L++ + S + P P +
Sbjct: 4 LEEDFAKILMLKEER----IKELEKRLSEKE-EEIQELKRKLHKCQS----VLPVPSTHI 54
Query: 433 VKGRRRRGAISAEVYTEE---DAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERS 603
R ISAE T D RK K ++ + +AI N +L+ ++
Sbjct: 55 GPRTTRAQGISAEPQTYRSFHDLRQAFRK-FTKSERSKDLIKEAILDNDFMKNLELSQIQ 113
Query: 604 DIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIY 783
+I D M+PV + +I++GD G YV++ G+++V ++G G FGELA++Y
Sbjct: 114 EIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILY 173
Query: 784 GTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTV 963
R ATVK NVKLW IDR ++ I+M + L K Y EFL V +SL +
Sbjct: 174 NCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPDEILSKL 233
Query: 964 ADALEPVQFEDGQKIVVQETRGRVLHI 1044
AD LE +E+G+ I+ Q RG I
Sbjct: 234 ADVLEETHYENGEYIIRQGARGDTFFI 260
Score = 57.0 bits (136), Expect = 3e-08
Identities = 38/121 (31%), Positives = 60/121 (49%), Gaps = 6/121 (4%)
Frame = +1
Query: 574 FSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNE------WA 735
F L D S + D + + GE +I+QG GD F++I +G+++V + +
Sbjct: 222 FQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGQVNVTREDSPSEDPVFL 281
Query: 736 TSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLS 915
++G+G FGE AL R A V A V IDRDS++ ++ G K YE+ +
Sbjct: 282 RTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEA 341
Query: 916 K 918
K
Sbjct: 342 K 342
>ref|NP_032952.2| protein kinase, cGMP-dependent, type II [Mus musculus]
Length = 762
Score = 106 bits (264), Expect = 5e-23
Identities = 75/275 (27%), Positives = 133/275 (48%), Gaps = 30/275 (10%)
Frame = +1
Query: 310 LREYFERLEKEE---AKQIQNLQKASARADSREDEI-----SPPPPNP------------ 429
L+E E+L K+ A+ + LQ + + +D I SP +P
Sbjct: 51 LKELREQLAKQTVAIAELTEELQSKCIQLNKLQDVIHVQGGSPLQASPDKVPLDVHRKTS 110
Query: 430 -VVKGRRRRGA---ISAEVYT------EEDAASYVRKVIPKDYKTMAALAKAIEKNVLFS 579
+V RRGA +SAE T + S+ + + KD + A+ KN
Sbjct: 111 GLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLK 170
Query: 580 HLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGS 759
LD + D+ + M+ ++ G VI+QG+ G++ +V+ +G ++V+ + +S+ +
Sbjct: 171 RLDPQQIKDMVECMYGRNYQQGSYVIKQGEPGNHIFVLAEGRLEVFQGEKLLSSIPMWTT 230
Query: 760 FGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESL 939
FGELA++Y R A+VKA TNVK W +DR+ ++ I+ + + + Y FL VS+L++L
Sbjct: 231 FGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEEYRNFLRSVSLLKNL 290
Query: 940 DKWERLTVADALEPVQFEDGQKIVVQETRGRVLHI 1044
+ + + D LE ++ G I+ + G I
Sbjct: 291 PEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFI 325
>ref|NP_659099.1| Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
[Mus musculus]
Length = 876
Score = 54.3 bits (129), Expect = 2e-07
Identities = 35/122 (28%), Positives = 66/122 (54%), Gaps = 3/122 (2%)
Frame = +1
Query: 577 SHLDDNERSDIFDAM-FPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEW-ATSVGE 750
+HL ++ + ++ + F AG + QGD+G ++Y+I +G ++V + + T++ E
Sbjct: 205 AHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHE 264
Query: 751 GGSFGELALIYGTPRAAT-VKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSI 927
G FG+LAL+ PRAAT + + N +D+ + RI+ + ++ EE V +
Sbjct: 265 GDDFGQLALVNDAPRAATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRL-EEHGKVVLV 323
Query: 928 LE 933
LE
Sbjct: 324 LE 325
>ref|NP_062662.1| cAMP-regulated guanine nucleotide exchange factor II [Mus musculus]
Length = 993
Score = 52.8 bits (125), Expect = 6e-07
Identities = 40/143 (27%), Positives = 73/143 (51%), Gaps = 6/143 (4%)
Frame = +1
Query: 577 SHLDDNERSDIFDAMFPVSFIAGETVI-QQGDEGDNFYVIDQGEMDVYVNNEWAT-SVGE 750
SHL + ++ + S G TV+ QG+EG ++Y+I +G ++V + + ++ E
Sbjct: 340 SHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHE 399
Query: 751 GGSFGELALIYGTPRAAT-VKAKTNVKLWGIDRDSYRRILM---GSTLRKRKMYEEFLSK 918
G FG+LAL+ PRAA+ V + N +D++ + RIL +T+R ++ ++ L
Sbjct: 400 GDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVL-- 457
Query: 919 VSILESLDKWERLTVADALEPVQ 987
+LE + R +P Q
Sbjct: 458 --VLEKVPAGNRAANQGNSQPQQ 478
Database: RefSeqMP
Posted date: Aug 2, 2006 12:58 AM
Number of letters in database: 21,768,885
Number of sequences in database: 45,328
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,319,380
Number of Sequences: 45328
Number of extensions: 1281336
Number of successful extensions: 5540
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 4558
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5501
length of database: 21,768,885
effective HSP length: 105
effective length of database: 17,009,445
effective search space used: 4235351805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)