BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006582
(1015 letters)
Database: RefSeqSP
1040 sequences; 434,620 total letters
Searching...done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_999238.1| 20-beta-hydroxysteroid dehydrogenase [Sus scrofa] 49 1e-07
Alignment gi|NP_999184.1| carbonyl reductase/NADP-retinol dehydrogenase [... 49 3e-07
>ref|NP_999238.1| 20-beta-hydroxysteroid dehydrogenase [Sus scrofa]
Length = 289
Score = 49.3 bits (116), Expect = 1e-07
Identities = 30/90 (33%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
Frame = +1
Query: 214 RVAIVTGGTEGIGFATAKHLAR-LGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
RVA+VTG +GIGFA + L R V++ + ++ + AV++++ E L+ + F D
Sbjct: 6 RVALVTGANKGIGFAIVRDLCRQFAGDVVLTARDVARGQAAVKQLQAEGLSPR--FHQLD 63
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGV 480
+ ++SIR + + L VLVNNA +
Sbjct: 64 IIDLQSIRALCDFLRKEYGGLDVLVNNAAI 93
>ref|NP_999184.1| carbonyl reductase/NADP-retinol dehydrogenase [Sus scrofa]
Length = 260
Score = 48.5 bits (114), Expect = 3e-07
Identities = 54/224 (24%), Positives = 88/224 (39%), Gaps = 3/224 (1%)
Frame = +1
Query: 202 PQPGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFL 381
P +VA+VT T+GIG A A+ LA+ G HV+++ V ++ E L+ V
Sbjct: 11 PLENKVALVTASTDGIGLAIARRLAQDGAHVVVSSRKQENVDRTVATLQGEGLS--VTGT 68
Query: 382 YCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQR---ETEDGFEEHFGVNYXXXX 552
C + + + V + +LV+NA V TE+ +++ VN
Sbjct: 69 VCHVGKAEDRERLVAMAVNLHGGVDILVSNAAVNPFFGNIIDATEEVWDKILHVNVKATV 128
Query: 553 XXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALV 732
+ G G S +V+ A H L G Y SK AL+
Sbjct: 129 LMTKAVVPEMEKRG--GGSVLIVSSVGAYHPFPNL--------------GPYNVSKTALL 172
Query: 733 LFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKK 864
+ L V L + + N PG++ T+ + V W + K+
Sbjct: 173 GLTKNLAVELAPRN--IRVNCLAPGLIKTN-FSQVLWMDKARKE 213
Database: RefSeqSP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 434,620
Number of sequences in database: 1040
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 694,297
Number of Sequences: 1040
Number of extensions: 14729
Number of successful extensions: 64
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 59
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 63
length of database: 434,620
effective HSP length: 77
effective length of database: 354,540
effective search space used: 92180400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqBP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006582
(1015 letters)
Database: RefSeqBP
33,508 sequences; 16,112,626 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_591168.2| PREDICTED: similar to dehydrogenase/reductase (... 319 2e-88
Alignment gi|XP_582319.2| PREDICTED: similar to retinol dehydrogenase 14 ... 167 9e-42
Alignment gi|NP_899207.1| double substrate-specificity short chain dehydr... 154 1e-37
Alignment gi|XP_592820.2| PREDICTED: similar to retinol dehydrogenase 11 ... 127 1e-29
Alignment gi|XP_584642.2| PREDICTED: similar to retinol dehydrogenase 11 ... 124 9e-29
Alignment gi|XP_870721.1| PREDICTED: similar to Retinol dehydrogenase 11 ... 117 1e-26
Alignment gi|XP_582373.2| PREDICTED: similar to Retinol dehydrogenase 11 ... 115 4e-26
Alignment gi|XP_584629.2| PREDICTED: similar to Retinol dehydrogenase 13,... 104 1e-22
Alignment gi|XP_583859.2| PREDICTED: similar to short chain dehydrogenase... 94 1e-19
Alignment gi|XP_876208.1| PREDICTED: similar to WW domain-containing oxid... 80 2e-15
>ref|XP_591168.2| PREDICTED: similar to dehydrogenase/reductase (SDR family) X-linked
[Bos taurus]
Length = 251
Score = 319 bits (817), Expect(2) = 2e-88
Identities = 155/203 (76%), Positives = 173/203 (85%)
Frame = +1
Query: 370 VEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXX 549
+EFLYCDLASM+SIR+FVQ FKMKK+PLHVLVNNAGVMMVPQR TEDGFEEHFGVNY
Sbjct: 5 MEFLYCDLASMRSIREFVQTFKMKKLPLHVLVNNAGVMMVPQRTTEDGFEEHFGVNYLGH 64
Query: 550 XXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLAL 729
Q SG+PG SARVVTVSSATHYVGEL+LDDLQSS YS H AYA+SKLAL
Sbjct: 65 FLLTNLLLDTLQESGAPGHSARVVTVSSATHYVGELNLDDLQSSTYYSAHAAYAQSKLAL 124
Query: 730 VLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAW 909
VLF++ LQ LL A+G PVTA+VADPGVV+TDLYR+VFWGTRL+KKL GWW F+TPDEGAW
Sbjct: 125 VLFTYHLQALLTAQGMPVTASVADPGVVDTDLYRYVFWGTRLVKKLLGWWVFKTPDEGAW 184
Query: 910 TSVYAAVSPALEGVGGRYLYTRR 978
TSVYAAV+PALEG+GGRYLY +
Sbjct: 185 TSVYAAVTPALEGLGGRYLYNEK 207
Score = 25.8 bits (55), Expect(2) = 2e-88
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3
Query: 969 HEKETKSLAITYDPK 1013
+EKET+SL TYDP+
Sbjct: 205 NEKETRSLEATYDPE 219
>ref|XP_582319.2| PREDICTED: similar to retinol dehydrogenase 14 (all-trans and
9-cis) [Bos taurus]
Length = 420
Score = 167 bits (424), Expect = 9e-42
Identities = 103/266 (38%), Positives = 143/266 (53%), Gaps = 15/266 (5%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLN--------- 363
G+ ++TG G+G ATA L RLG VI+ + +A EA ++R E
Sbjct: 127 GKTVLITGANSGLGRATAAELLRLGARVIMGCRDRERAEEAAGQLRREVCPAGGPDSGPN 186
Query: 364 ----DKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFG 531
++ DLAS+ S+R F Q+ ++ L VL+NNAGV P +TEDGFE FG
Sbjct: 187 SGGAGELVVKELDLASLSSVRSFCQEMLQEEPRLDVLINNAGVFQCPYMKTEDGFEMQFG 246
Query: 532 VNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYA 711
VN+ ++S +R+V VSS + G+++ +DL S Y+ Y+
Sbjct: 247 VNHLGHFLLTNLLLGLLKSSA----PSRIVVVSSKLYKYGDINFEDLNSEQSYNKSFCYS 302
Query: 712 RSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWG--TRLLKKLFGWWFF 885
RSKLA +LF+ L LE G+ VT NV PG+V T+L RH+ R L L W FF
Sbjct: 303 RSKLANILFTRELARRLE--GTSVTVNVLHPGIVRTNLGRHIHIPLLVRPLFNLVSWAFF 360
Query: 886 QTPDEGAWTSVYAAVSPALEGVGGRY 963
+TP+EGA T+VY A SP +EGV GRY
Sbjct: 361 KTPEEGAQTAVYLASSPEVEGVSGRY 386
>ref|NP_899207.1| double substrate-specificity short chain dehydrogenase/reductase 2
[Bos taurus]
Length = 316
Score = 154 bits (388), Expect = 1e-37
Identities = 99/251 (39%), Positives = 136/251 (54%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G+V ++TG GIG TA+ LAR G V IA + K A +I+ +T N +V D
Sbjct: 39 GKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLD 98
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
L+ KSIR F + F ++ LH+L+NNAGVM+ P +T DGFE H VN+
Sbjct: 99 LSDTKSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLL 158
Query: 571 XXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHRL 750
+ S ARVV +SS H++G++ DLQ Y+ AY SKLA VLF+ L
Sbjct: 159 LGRLKESA----PARVVNLSSVAHHLGKIRFHDLQGDKYYNLGFAYCHSKLANVLFTREL 214
Query: 751 QVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAAV 930
L KG+ VT PG+V + L RH F LL +LF F +T EGA TS++ A+
Sbjct: 215 AKRL--KGTGVTTYAVHPGIVRSKLVRHSFL-LCLLWRLFS-PFLKTTWEGAQTSLHCAL 270
Query: 931 SPALEGVGGRY 963
+ LE + G+Y
Sbjct: 271 AEGLEPLSGKY 281
>ref|XP_592820.2| PREDICTED: similar to retinol dehydrogenase 11 (predicted) [Bos
taurus]
Length = 377
Score = 127 bits (319), Expect = 1e-29
Identities = 92/254 (36%), Positives = 126/254 (49%), Gaps = 3/254 (1%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
GR A+VTG GIG TA LAR G V++A + + A +R+E+ N++V F+ D
Sbjct: 36 GRTAVVTGANSGIGKMTALELARRGARVVLACRSRERGEAAAFDLRQESGNNEVIFMALD 95
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
LAS+ S+R F F + L +L++NAG+ + T + F VN+
Sbjct: 96 LASLASVRAFATAFLSSEPRLDILIHNAGISSCGR--TREPFNLLLRVNHIGPFLLTHLL 153
Query: 571 XXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSS--GGYSPHGAYARSKLALVLFSH 744
+ S +RVV VSSA H G LD L G AYA SKLA VLF+
Sbjct: 154 LPRLKTSA----PSRVVVVSSAAHRRGRLDFTRLDHPVVGWQQELRAYANSKLANVLFAR 209
Query: 745 RLQVLLEAKGSPVTANVADPGVVNTDLY-RHVFWGTRLLKKLFGWWFFQTPDEGAWTSVY 921
L LE G+ VT A PG VN++L+ RHV R L + W + P GA T +Y
Sbjct: 210 ELATQLE--GTGVTCYAAHPGPVNSELFLRHVPGWLRPLLRPLAWLVLRAPRGGAQTPLY 267
Query: 922 AAVSPALEGVGGRY 963
A+ +E + GRY
Sbjct: 268 CALQEGIEPLSGRY 281
>ref|XP_584642.2| PREDICTED: similar to retinol dehydrogenase 11 (predicted) [Bos
taurus]
Length = 330
Score = 124 bits (312), Expect = 9e-29
Identities = 85/252 (33%), Positives = 131/252 (51%), Gaps = 1/252 (0%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G+ A+VTG GIG A ++ LA G VI+A + + ++A+ +I+ + ++++ D
Sbjct: 49 GKTAVVTGANSGIGKAVSQELAHRGARVILACRSRERGQQALAEIQATSKSNRLLLGEVD 108
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
L+SM SIR F Q+ + +H+LVNNA V P T +G + F NY
Sbjct: 109 LSSMASIRSFAQRLLQECPEIHLLVNNAAVCGFPTTLTPEGLDLTFATNYTGPFLLTNLL 168
Query: 571 XXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSG-GYSPHGAYARSKLALVLFSHR 747
Q +G SARVV VSS G +D D L +G + + Y SKL L F+ +
Sbjct: 169 QGALQRAG----SARVVNVSSFRQSHGYIDEDHLIGAGRPLTFNQNYDCSKLLLASFTGK 224
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAA 927
L L+ G+ VT N DPGVV T + +H W R L L +FF+ +GA +Y +
Sbjct: 225 LAQRLQ--GTGVTVNSVDPGVVYTKIMKHFSWSYRFLFWLLS-FFFKDSKQGAVPVLYLS 281
Query: 928 VSPALEGVGGRY 963
++ L+G+ G++
Sbjct: 282 LAKELDGISGKH 293
>ref|XP_870721.1| PREDICTED: similar to Retinol dehydrogenase 11 (Retinal reductase
1) (RalR1) (Prostate short-chain dehydrogenase/reductase
1) (Androgen-regulated short-chain
dehydrogenase/reductase 1) (HCV core-binding protein
HCBP12) isoform 2 [Bos taurus]
Length = 314
Score = 117 bits (293), Expect = 1e-26
Identities = 85/256 (33%), Positives = 123/256 (48%), Gaps = 4/256 (1%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+VA+VTG GIG TAK LAR G V +A + R+I+ T N +V
Sbjct: 14 PGKVAVVTGANAGIGKETAKELARRGARVYLACRDVQNGELVAREIQMMTGNQQVLVRKL 73
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DLA KSIR F ++F +H++ ++ ++ + G + Y
Sbjct: 74 DLADTKSIRAFAKRFLEDHKYIHLINSSPHFLICGFPGSSAGKDSACNTGYLGHFLLTHL 133
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S +RVV VSS H +G + +LQ Y AY SKLA +LF+
Sbjct: 134 LLEKLEESA----PSRVVNVSSLAHLLGRIHFHNLQGEKFYQSGLAYCHSKLANILFTQE 189
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWW----FFQTPDEGAWTS 915
L L KGS VT PG VN++L RH + L++ + WW F +TP +GA TS
Sbjct: 190 LARRL--KGSGVTVYSVHPGTVNSELVRH----SALMRWI--WWIFSFFIKTPQQGAQTS 241
Query: 916 VYAAVSPALEGVGGRY 963
+Y A++ LE + G +
Sbjct: 242 LYCALTEGLEVLSGNH 257
>ref|XP_582373.2| PREDICTED: similar to Retinol dehydrogenase 11 (Retinal reductase
1) (RalR1) (Prostate short-chain dehydrogenase/reductase
1) (Androgen-regulated short-chain
dehydrogenase/reductase 1) (HCV core-binding protein
HCBP12) isoform 1 [Bos taurus]
Length = 308
Score = 115 bits (289), Expect = 4e-26
Identities = 88/256 (34%), Positives = 122/256 (47%), Gaps = 4/256 (1%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+VA+VTG GIG TAK LAR G V +A + R+I+ T N +V
Sbjct: 14 PGKVAVVTGANAGIGKETAKELARRGARVYLACRDVQNGELVAREIQMMTGNQQVLVRKL 73
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DLA KSIR F ++F +H L+N+ G + G + Y
Sbjct: 74 DLADTKSIRAFAKRFLEDHKYIH-LINSVGF-----PGSSAGKDSACNTGYLGHFLLTHL 127
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S +RVV VSS H +G + +LQ Y AY SKLA +LF+
Sbjct: 128 LLEKLEESA----PSRVVNVSSLAHLLGRIHFHNLQGEKFYQSGLAYCHSKLANILFTQE 183
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWW----FFQTPDEGAWTS 915
L L KGS VT PG VN++L RH + L++ + WW F +TP +GA TS
Sbjct: 184 LARRL--KGSGVTVYSVHPGTVNSELVRH----SALMRWI--WWIFSFFIKTPQQGAQTS 235
Query: 916 VYAAVSPALEGVGGRY 963
+Y A++ LE + G +
Sbjct: 236 LYCALTEGLEVLSGNH 251
>ref|XP_584629.2| PREDICTED: similar to Retinol dehydrogenase 13, partial [Bos
taurus]
Length = 148
Score = 104 bits (259), Expect = 1e-22
Identities = 59/130 (45%), Positives = 77/130 (59%)
Frame = +1
Query: 151 AQLLRRCRGGFAEPVFPPQPGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKARE 330
A LL+ G A P PG+ IVTG GIG TA LA+ G ++I+A + K
Sbjct: 18 AVLLKDFVAGGACPSKATIPGKTVIVTGANTGIGKQTALELAKRGGNIILACRDMEKCEA 77
Query: 331 AVRKIREETLNDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETED 510
A ++IR ETLN +V + DLAS+KSIR+F K ++ +H+L+NNA VM P TED
Sbjct: 78 AAKEIRGETLNHRVNARHLDLASLKSIREFAAKVTEEEEHVHILINNAAVMRCPHWTTED 137
Query: 511 GFEEHFGVNY 540
GFE GVNY
Sbjct: 138 GFEMQLGVNY 147
>ref|XP_583859.2| PREDICTED: similar to short chain dehydrogenase/reductase [Bos
taurus]
Length = 317
Score = 94.4 bits (233), Expect = 1e-19
Identities = 82/268 (30%), Positives = 125/268 (46%), Gaps = 8/268 (2%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PGR +VTGG GIG ATA +A+ G V + + S+A A +I E+ N +
Sbjct: 39 PGRAFMVTGGNSGIGKATAMEIAKRGGTVHLVCRDHSRAEGAKAEIIRESGNQNIFLHIV 98
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DL+ KS+ +FV+ FK + L+VL+NNAG M+ + TEDG E++F N
Sbjct: 99 DLSLPKSVWKFVENFKQEH-TLNVLINNAGCMVNKRELTEDGLEKNFATNTLGVYVLTTA 157
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSS-GGYSPHGAYARSKLALVLFSH 744
+ P RV+TVSS V +L+ DD QS + YA++K V+ +
Sbjct: 158 LIPVLEKEHDP----RVITVSSGGMLVQKLNTDDPQSERTAFDGTMVYAQNKRQQVVLTE 213
Query: 745 RLQVLLEAKGSP-VTANVADPGVVNTDLYR------HVFWGTRLLKKLFGWWFFQTPDEG 903
R A+ P + + PG V+T R H G RL ++ +G
Sbjct: 214 RW-----ARAHPAIHFSCMHPGWVDTPGVRLSMPGFHARLGARL----------RSEAQG 258
Query: 904 AWTSVYAAVSPALEGVGGRYLYTRRRPS 987
A T ++ A++PA + R+P+
Sbjct: 259 ADTVLWLALAPAATAQPSGCFFQDRKPA 286
>ref|XP_876208.1| PREDICTED: similar to WW domain-containing oxidoreductase isoform 1
[Bos taurus]
Length = 745
Score = 80.5 bits (197), Expect = 2e-15
Identities = 73/225 (32%), Positives = 100/225 (44%), Gaps = 11/225 (4%)
Frame = +1
Query: 367 KVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXX 546
KVE + DLA ++S++ F Q FK K + LHVLV NA V +P T+DG E F VN+
Sbjct: 7 KVEAMTLDLALLRSVQHFAQAFKAKNVSLHVLVCNAAVFGLPWTLTKDGLETTFQVNHLG 66
Query: 547 XXXXXXXXXXXXQASGSPGRSARVVTVSSATHYV-------GELDLDDLQ-SSGGYSPHG 702
S ARVV VSS +H G+LD L S Y
Sbjct: 67 HFYLVQLLQDVLCRSA----PARVVVVSSESHRFTDINDSSGKLDFSRLSPSKNDYWAML 122
Query: 703 AYARSKLALVLFSHRLQVLLEAKGSPVTANVADPG-VVNTDLYRHVFWGTRLLKKLFGWW 879
AY RSKL +LFS+ L L +G VT+N PG ++ + L+R GWW
Sbjct: 123 AYNRSKLCNILFSNELHRRLSPRG--VTSNAVHPGNMMYSALHR-------------GWW 167
Query: 880 FFQTPDEGAWTSVYAAVSPALEGVGGRY--LYTRRRPSHWRSRTT 1008
+T ++ P + + + LY + S W +TT
Sbjct: 168 --------VYTLLFTLARPFTKSMLEAHFILYFTKGKSFWVLQTT 204
Database: RefSeqBP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 16,112,626
Number of sequences in database: 33,508
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,671,017
Number of Sequences: 33508
Number of extensions: 589721
Number of successful extensions: 2656
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 2399
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2617
length of database: 16,112,626
effective HSP length: 102
effective length of database: 12,694,810
effective search space used: 2983280350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqCP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006582
(1015 letters)
Database: RefSeqCP
33,732 sequences; 19,266,565 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_852222.1| PREDICTED: similar to dehydrogenase/reductase (... 421 e-119
Alignment gi|XP_854127.1| PREDICTED: similar to Retinol dehydrogenase 13 ... 167 2e-41
Alignment gi|XP_540096.2| PREDICTED: similar to retinol dehydrogenase 14 ... 163 2e-40
Alignment gi|XP_547866.2| PREDICTED: similar to retinol dehydrogenase 12 ... 162 5e-40
Alignment gi|XP_854354.1| PREDICTED: similar to Retinol dehydrogenase 11 ... 159 3e-39
Alignment gi|XP_548293.2| PREDICTED: similar to retinol dehydrogenase 11 ... 122 6e-28
Alignment gi|XP_857582.1| PREDICTED: similar to retinol dehydrogenase 14 ... 120 2e-27
Alignment gi|XP_852623.1| PREDICTED: similar to WW domain-containing oxid... 120 3e-27
Alignment gi|XP_533000.2| PREDICTED: similar to Retinol dehydrogenase 12 ... 116 3e-26
Alignment gi|XP_852675.1| PREDICTED: similar to Carbonyl reductase [NADPH... 68 2e-11
>ref|XP_852222.1| PREDICTED: similar to dehydrogenase/reductase (SDR family) X-linked
[Canis familiaris]
Length = 387
Score = 421 bits (1082), Expect(2) = e-119
Identities = 205/272 (75%), Positives = 229/272 (84%)
Frame = +1
Query: 163 RRCRGGFAEPVFPPQPGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRK 342
R R P PQP RVAIVTGGT+GIG++TAKHLARLGMHVI+AGNNDS A + VRK
Sbjct: 79 RFSRVHMGNPFLAPQPERVAIVTGGTDGIGYSTAKHLARLGMHVILAGNNDSSAPDVVRK 138
Query: 343 IREETLNDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEE 522
I+EETLNDKVEFLYCDLAS++SIRQFVQKFK KKIPLHVLVNNAGVMMVP+R TEDGFEE
Sbjct: 139 IQEETLNDKVEFLYCDLASLRSIRQFVQKFKKKKIPLHVLVNNAGVMMVPERTTEDGFEE 198
Query: 523 HFGVNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHG 702
HFG+NY + SG+PGR ARVVTVSSATHY+GELD+DDLQ S YSPH
Sbjct: 199 HFGLNYLGHFLLTNLLLDTLKESGAPGRCARVVTVSSATHYIGELDMDDLQGSRCYSPHA 258
Query: 703 AYARSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWF 882
AYA+SKLALVLF++ LQ LL A+GSPVTANV DPGVVNT+LYRHVFWGTRL+KKLFGWWF
Sbjct: 259 AYAQSKLALVLFTYHLQRLLAAQGSPVTANVVDPGVVNTNLYRHVFWGTRLIKKLFGWWF 318
Query: 883 FQTPDEGAWTSVYAAVSPALEGVGGRYLYTRR 978
F+TPDEGAWTSVYAAV+P LEG+GGRYLY +
Sbjct: 319 FKTPDEGAWTSVYAAVTPDLEGLGGRYLYNEK 350
Score = 27.3 bits (59), Expect(2) = e-119
Identities = 11/13 (84%), Positives = 13/13 (100%)
Frame = +3
Query: 969 HEKETKSLAITYD 1007
+EKETKSLA+TYD
Sbjct: 348 NEKETKSLAVTYD 360
>ref|XP_854127.1| PREDICTED: similar to Retinol dehydrogenase 13 [Canis familiaris]
Length = 334
Score = 167 bits (423), Expect = 2e-41
Identities = 109/277 (39%), Positives = 146/277 (52%), Gaps = 6/277 (2%)
Frame = +1
Query: 151 AQLLRRCRGGFAEPVFPPQPGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKARE 330
A LLR G A P PG+ IVTG GIG TA LAR G ++I+A + K
Sbjct: 18 AVLLRDYIAGGACPSKATIPGKTVIVTGANTGIGKQTALELARRGGNIILACRDMEKCEA 77
Query: 331 AVRKIREETLNDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETED 510
A ++IR ETLN +V + DLAS+KSIR+F K ++ +H+LVNNA VM P T+D
Sbjct: 78 AAKEIRGETLNHRVSAWHLDLASLKSIREFAAKIIEEEEQVHILVNNAAVMRCPHWTTKD 137
Query: 511 GFEEHFGVNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQ-SSGG 687
GFE FGVN+ +AS +R++ +SS H G +D DDL
Sbjct: 138 GFEMQFGVNHLGHFLLTNLLLDKLKASA----PSRIINLSSLAHVAGHIDFDDLNWEKRK 193
Query: 688 YSPHGAYARSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHV-----FWGTR 852
Y AY +SKLA++LF+ L L+ G+ VT N PGV T+L RH + +
Sbjct: 194 YDTKAAYCQSKLAVILFTKELSRRLQ--GTGVTVNALHPGVARTELGRHTGMHSSAFSSF 251
Query: 853 LLKKLFGWWFFQTPDEGAWTSVYAAVSPALEGVGGRY 963
L +F W ++P A S Y AV+ LEGV G+Y
Sbjct: 252 TLGPIF-WLLVKSPQLAAQPSTYLAVAEELEGVSGKY 287
>ref|XP_540096.2| PREDICTED: similar to retinol dehydrogenase 14 (all-trans and
9-cis) isoform 1 [Canis familiaris]
Length = 336
Score = 163 bits (413), Expect = 2e-40
Identities = 102/266 (38%), Positives = 143/266 (53%), Gaps = 15/266 (5%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLN--------- 363
G+ ++TG G+G ATA L RLG VI+ + ++A EA ++R E
Sbjct: 43 GKTVLITGANSGLGRATAAALLRLGARVIMGCRDRARAEEAAGQLRRELRQAGGREPGSD 102
Query: 364 ----DKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFG 531
++ DLAS++S+R F Q+ ++ L VL+NNAG+ P +TEDGFE FG
Sbjct: 103 VGAAGELVVRELDLASLRSVRAFCQEVLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFG 162
Query: 532 VNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYA 711
VN+ + S +R+V VSS + G+++ +DL S Y+ Y+
Sbjct: 163 VNHLGHFLLTNLLLGLLKNSA----PSRIVVVSSKLYKYGDINFEDLNSEQSYNKSFCYS 218
Query: 712 RSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWG--TRLLKKLFGWWFF 885
RSKLA +LF+ L LE G+ VT NV PG+V T+L RH+ R L L W FF
Sbjct: 219 RSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVRPLFNLVSWAFF 276
Query: 886 QTPDEGAWTSVYAAVSPALEGVGGRY 963
+TP EGA TSVY A SP +EGV G+Y
Sbjct: 277 KTPVEGAQTSVYLASSPEVEGVSGKY 302
>ref|XP_547866.2| PREDICTED: similar to retinol dehydrogenase 12 (all-trans and
9-cis) [Canis familiaris]
Length = 303
Score = 162 bits (410), Expect = 5e-40
Identities = 102/258 (39%), Positives = 142/258 (55%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+V ++TG GIG TA+ LAR G V IA + K A +IR +T N +V
Sbjct: 25 PGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKL 84
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DL+ KSIR F + F ++ LH+L+NNAGVMM P +T DGFE H GVN+
Sbjct: 85 DLSDTKSIRAFAEGFLAEEKQLHILINNAGVMMCPYSKTADGFETHLGVNHLGHFLLTHL 144
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S ARVV +SS H++G++ DLQ YS AY SKLA +LF+
Sbjct: 145 LLERLKES----TPARVVNLSSVAHHIGKIHFHDLQGEKRYSRGFAYCHSKLANMLFTRE 200
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAA 927
L L+ G+ VT PGVV+++L RH F LL ++F F ++ EGA TS++ A
Sbjct: 201 LAKRLQ--GTGVTTYAVHPGVVSSELVRHSFL-LCLLWRIFS-PFVKSAREGAQTSLHCA 256
Query: 928 VSPALEGVGGRYLYTRRR 981
++ LE + G+Y +R
Sbjct: 257 LAEGLEPLSGKYFSDCKR 274
>ref|XP_854354.1| PREDICTED: similar to Retinol dehydrogenase 11 (Retinal reductase
1) (RalR1) (Prostate short-chain dehydrogenase/reductase
1) (Androgen-regulated short-chain
dehydrogenase/reductase 1) (HCV core-binding protein
HCBP12) [Canis familiaris]
Length = 337
Score = 159 bits (403), Expect = 3e-39
Identities = 101/256 (39%), Positives = 138/256 (53%), Gaps = 4/256 (1%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+VA+VTG GIG TAK LA+ G V +A + K R+I+ T N +V
Sbjct: 59 PGKVAVVTGANTGIGKETAKELAQRGARVYLACRDVLKGELVAREIQTMTGNKQVLVRKL 118
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DLA KSIR F + F ++ LH+L+NNAGVMM P +T DGFE H GVN+
Sbjct: 119 DLADTKSIRAFAKGFLAEEKHLHILINNAGVMMCPYTKTVDGFEMHMGVNHLGHFLLTHL 178
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S +R+V VSS H++G + DLQ Y+ AY SKLA +LF+
Sbjct: 179 LLEKLKESA----PSRIVNVSSLAHHLGRIHFHDLQGEKFYNSGLAYCHSKLANILFTQE 234
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWW----FFQTPDEGAWTS 915
L L KGS +TA PG V ++L RH + +K + WW F +TP +GA TS
Sbjct: 235 LARRL--KGSGITAYSVHPGTVKSELVRH----SPFMKWM--WWLFSFFIKTPQQGAQTS 286
Query: 916 VYAAVSPALEGVGGRY 963
+Y A++ LE + G +
Sbjct: 287 LYCAITEGLEVLSGHH 302
>ref|XP_548293.2| PREDICTED: similar to retinol dehydrogenase 11 (predicted) [Canis
familiaris]
Length = 377
Score = 122 bits (306), Expect = 6e-28
Identities = 93/255 (36%), Positives = 126/255 (49%), Gaps = 4/255 (1%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
GR A+VTG GIG TA LAR G V++A + + A +R+E+ N++V F+ D
Sbjct: 36 GRTAVVTGANSGIGKMTALELARRGARVVLACRSRERGEAAAFDLRQESGNNEVIFMALD 95
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
LAS+ S+R F F + L +L++NAG+ + T F VN+
Sbjct: 96 LASLASVRAFATAFLSSEPRLDILIHNAGISSCGR--THKPFNLLLRVNHIGPFLLTHLL 153
Query: 571 XXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSS--GGYSPHGAYARSKLALVLFSH 744
+ +RVV VSSA H G LD L G AYA SKLA VLF+
Sbjct: 154 LPRLKTCA----PSRVVVVSSAAHRRGRLDFTRLDRPVVGWQQELRAYADSKLANVLFAR 209
Query: 745 RLQVLLEAKGSPVTANVADPGVVNTDLY-RHV-FWGTRLLKKLFGWWFFQTPDEGAWTSV 918
L LE G+ VT A PG VN++L+ RHV W LL+ L W + P GA T +
Sbjct: 210 ELATQLE--GTGVTCYAAHPGPVNSELFLRHVPGWLCPLLRPL-AWLMLRAPRGGAQTPL 266
Query: 919 YAAVSPALEGVGGRY 963
Y A+ +E + GRY
Sbjct: 267 YCALQEGIEPLSGRY 281
>ref|XP_857582.1| PREDICTED: similar to retinol dehydrogenase 14 (all-trans and
9-cis) isoform 4 [Canis familiaris]
Length = 307
Score = 120 bits (301), Expect = 2e-27
Identities = 88/266 (33%), Positives = 124/266 (46%), Gaps = 15/266 (5%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLN--------- 363
G+ ++TG G+G ATA L RLG VI+ + ++A EA ++R E
Sbjct: 43 GKTVLITGANSGLGRATAAALLRLGARVIMGCRDRARAEEAAGQLRRELRQAGGREPGSD 102
Query: 364 ----DKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFG 531
++ DLAS++S+R F Q+ FG
Sbjct: 103 VGAAGELVVRELDLASLRSVRAFCQEVLQ-----------------------------FG 133
Query: 532 VNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYA 711
VN+ + S +R+V VSS + G+++ +DL S Y+ Y+
Sbjct: 134 VNHLGHFLLTNLLLGLLKNSAP----SRIVVVSSKLYKYGDINFEDLNSEQSYNKSFCYS 189
Query: 712 RSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWG--TRLLKKLFGWWFF 885
RSKLA +LF+ L LE G+ VT NV PG+V T+L RH+ R L L W FF
Sbjct: 190 RSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVRPLFNLVSWAFF 247
Query: 886 QTPDEGAWTSVYAAVSPALEGVGGRY 963
+TP EGA TSVY A SP +EGV G+Y
Sbjct: 248 KTPVEGAQTSVYLASSPEVEGVSGKY 273
>ref|XP_852623.1| PREDICTED: similar to WW domain-containing oxidoreductase isoform 1
[Canis familiaris]
Length = 383
Score = 120 bits (300), Expect = 3e-27
Identities = 87/224 (38%), Positives = 110/224 (49%), Gaps = 8/224 (3%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G+V +VTG GIGF TAK A G HVI+A N ++A EAV +I E KVE + D
Sbjct: 124 GKVVVVTGANSGIGFETAKSFALHGAHVILACRNMTRANEAVSQILGEWHKAKVEAMTLD 183
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
LA ++S++ F Q FK K + LHVLV NA +P T+DG E F VN+
Sbjct: 184 LALLRSVQHFAQAFKAKNVSLHVLVCNAAAFALPWSLTKDGLETTFQVNHLGHFYLVQLL 243
Query: 571 XXXXQASGSPGRSARVVTVSSATHYV-------GELDLDDLQ-SSGGYSPHGAYARSKLA 726
S ARVV VSS +H G+LD L S Y AY RSKL
Sbjct: 244 QDVLCRSA----PARVVVVSSESHRFTDINDSSGKLDFSRLSPSKSDYWAMLAYNRSKLC 299
Query: 727 LVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLL 858
+LFS+ L L +G VT+N PG + +W LL
Sbjct: 300 NILFSNELHRRLSPRG--VTSNAVHPGNMMYSSIHRNWWVYTLL 341
>ref|XP_533000.2| PREDICTED: similar to Retinol dehydrogenase 12 [Canis familiaris]
Length = 596
Score = 116 bits (291), Expect = 3e-26
Identities = 86/252 (34%), Positives = 127/252 (50%), Gaps = 1/252 (0%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G+ A+VTG GIG A + LAR G VI+A N + ++A+ +I+ + + D
Sbjct: 315 GKTAVVTGANSGIGKAVCQELARRGARVILACRNWERGQKALAEIQVASKGTCLLLGQVD 374
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
L+SM SIR F + + +H+LVNNA + P+ T +G + F NY
Sbjct: 375 LSSMASIRSFARWLLQEYPEIHLLVNNAAISGFPKTLTPEGLDLTFATNYVGPFLLTNLL 434
Query: 571 XXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSG-GYSPHGAYARSKLALVLFSHR 747
Q +G SARVV VSS H G +D L +G + +Y SKL L F+
Sbjct: 435 QGALQRAG----SARVVNVSSFRHAHGYVDEKHLTGAGKPLNLIQSYDCSKLLLTSFTGE 490
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAA 927
L L+ G+ VT N DPGVV T++ + W R L LF +F + +GA +Y +
Sbjct: 491 LARRLQ--GTGVTVNSVDPGVVYTEIMKPYPWLYRFLFWLFS-FFCKDVKQGAIPVLYLS 547
Query: 928 VSPALEGVGGRY 963
++ L+GV G+Y
Sbjct: 548 LAKELDGVSGKY 559
>ref|XP_852675.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) (Prostaglandin-E(2) 9-reductase)
(Prostaglandin 9-ketoreductase) (15-hydroxyprostaglandin
dehydrogenase [NADP+]) [Canis familiaris]
Length = 277
Score = 67.8 bits (164), Expect = 2e-11
Identities = 76/290 (26%), Positives = 124/290 (42%), Gaps = 35/290 (12%)
Frame = +1
Query: 214 RVAIVTGGTEGIGFATAKHLAR-LGMHVIIAGNNDSKAREAVRKIREETLNDKVEFL-YC 387
RVA+VTG +GIGFA A+ L R V++ ++++ R AV++++ E L+ + L
Sbjct: 6 RVALVTGANKGIGFAIARELCRQFSGDVVLTARDEARGRAAVQQLQAEGLSPRFHLLDID 65
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DL S++++R F++K + L VLVNNAG+ +T D H
Sbjct: 66 DLQSIRALRDFLRK---EYGGLDVLVNNAGIAF----KTNDPTPFHIQAEVTMKTNFFGT 118
Query: 568 XXXXXQASGSPGRSARVVTVSSA------------------THYVGELDLDDLQS----- 678
+ RVV VSS + + E +L L +
Sbjct: 119 RDVCTELLPLMKPQGRVVNVSSVVSVRALKSCSPELQQKFRSEAITEEELVGLMNKFVED 178
Query: 679 -------SGGYSPHGAYARSKLALVLFS--HRLQVLLEAKGSPVTANVADPGVVNTDLYR 831
+ G+ P AY +K+ + + S H ++ + + + N PG V TD+
Sbjct: 179 TKKGVHRNEGW-PDNAYGVTKIGVTVLSRIHARKLSEQRRDDKILLNACCPGWVRTDMA- 236
Query: 832 HVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAAVSPA-LEGVGGRYLYTRR 978
G R K +P+EGA T VY A+ P+ EG G +L ++
Sbjct: 237 ----GPRAPK---------SPEEGAETPVYLALLPSDAEGPHGEFLMEKK 273
Database: RefSeqCP
Posted date: Aug 1, 2006 9:25 PM
Number of letters in database: 19,266,565
Number of sequences in database: 33,732
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,373,068
Number of Sequences: 33732
Number of extensions: 691329
Number of successful extensions: 3170
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 2809
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3122
length of database: 19,266,565
effective HSP length: 103
effective length of database: 15,792,169
effective search space used: 3695367546
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqHP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006582
(1015 letters)
Database: RefSeqHP
39,411 sequences; 17,774,539 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_660160.1| dehydrogenase/reductase (SDR family) X-linked [... 443 e-125
Alignment gi|NP_065956.1| retinol dehydrogenase 14 (all-trans and 9-cis) ... 167 1e-41
Alignment gi|NP_689656.1| retinol dehydrogenase 12 (all-trans and 9-cis) ... 163 2e-40
Alignment gi|NP_057110.2| androgen-regulated short-chain dehydrogenase/re... 151 1e-36
Alignment gi|NP_057457.1| WW domain-containing oxidoreductase isoform 1 [... 144 1e-34
Alignment gi|NP_612421.1| retinol dehydrogenase 13 (all-trans and 9-cis) ... 132 4e-31
Alignment gi|NP_653284.1| hypothetical protein LOC147015 [Homo sapiens] 107 1e-23
Alignment gi|XP_371503.3| PREDICTED: similar to short-chain dehydrogenase... 72 1e-12
Alignment gi|XP_947127.1| PREDICTED: similar to short-chain dehydrogenase... 72 1e-12
Alignment gi|NP_005785.1| dehydrogenase/reductase (SDR family) member 2 i... 62 1e-09
>ref|NP_660160.1| dehydrogenase/reductase (SDR family) X-linked [Homo sapiens]
Length = 330
Score = 443 bits (1140), Expect(2) = e-125
Identities = 217/278 (78%), Positives = 239/278 (85%)
Frame = +1
Query: 145 ILAQLLRRCRGGFAEPVFPPQPGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKA 324
ILAQLLRRCRGGF EPVFPP+P RVAIVTGGT+GIG++TAKHLARLGMHVIIAGNNDSKA
Sbjct: 21 ILAQLLRRCRGGFLEPVFPPRPDRVAIVTGGTDGIGYSTAKHLARLGMHVIIAGNNDSKA 80
Query: 325 REAVRKIREETLNDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRET 504
++ V KI+EETLNDKVEFLYCDLASM SIRQFVQKFKMKKIPLHVL+NNAGVMMVPQR+T
Sbjct: 81 KQVVSKIKEETLNDKVEFLYCDLASMTSIRQFVQKFKMKKIPLHVLINNAGVMMVPQRKT 140
Query: 505 EDGFEEHFGVNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSG 684
DGFEEHFG+NY + SGSPG SARVVTVSSATHYV EL++DDLQSS
Sbjct: 141 RDGFEEHFGLNYLGHFLLTNLLLDTLKESGSPGHSARVVTVSSATHYVAELNMDDLQSSA 200
Query: 685 GYSPHGAYARSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKK 864
YSPH AYA+SKLALVLF++ LQ LL A+GS VTANV DPGVVNTDLY+HVFW TRL KK
Sbjct: 201 CYSPHAAYAQSKLALVLFTYHLQRLLAAEGSHVTANVVDPGVVNTDLYKHVFWATRLAKK 260
Query: 865 LFGWWFFQTPDEGAWTSVYAAVSPALEGVGGRYLYTRR 978
L GW F+TPDEGAWTS+YAAV+P LEGVGGRYLY +
Sbjct: 261 LLGWLLFKTPDEGAWTSIYAAVTPELEGVGGRYLYNEK 298
Score = 24.6 bits (52), Expect(2) = e-125
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3
Query: 969 HEKETKSLAITYDPK 1013
+EKETKSL +TY+ K
Sbjct: 296 NEKETKSLHVTYNQK 310
>ref|NP_065956.1| retinol dehydrogenase 14 (all-trans and 9-cis) [Homo sapiens]
Length = 336
Score = 167 bits (423), Expect = 1e-41
Identities = 104/267 (38%), Positives = 146/267 (54%), Gaps = 16/267 (5%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLN--------- 363
G+ ++TG G+G ATA L RLG VI+ + ++A EA ++R E
Sbjct: 43 GKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPEPG 102
Query: 364 ----DKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFG 531
++ DLAS++S+R F Q+ ++ L VL+NNAG+ P +TEDGFE FG
Sbjct: 103 VSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFG 162
Query: 532 VNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYA 711
VN+ ++S +R+V VSS + G+++ DDL S Y+ Y+
Sbjct: 163 VNHLGHFLLTNLLLGLLKSSA----PSRIVVVSSKLYKYGDINFDDLNSEQSYNKSFCYS 218
Query: 712 RSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLF---GWWF 882
RSKLA +LF+ L LE G+ VT NV PG+V T+L RH+ L+K LF W F
Sbjct: 219 RSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHI-PLLVKPLFNLVSWAF 275
Query: 883 FQTPDEGAWTSVYAAVSPALEGVGGRY 963
F+TP EGA TS+Y A SP +EGV GRY
Sbjct: 276 FKTPVEGAQTSIYLASSPEVEGVSGRY 302
>ref|NP_689656.1| retinol dehydrogenase 12 (all-trans and 9-cis) [Homo sapiens]
Length = 316
Score = 163 bits (413), Expect = 2e-40
Identities = 108/269 (40%), Positives = 144/269 (53%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+V ++TG GIG TA+ LA G V IA + K A +IR +T N +V
Sbjct: 38 PGKVVVITGANTGIGKETARELASRGARVYIACRDVLKGESAASEIRVDTKNSQVLVRKL 97
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DL+ KSIR F + F ++ LH+L+NNAGVMM P +T DGFE H GVN+
Sbjct: 98 DLSDTKSIRAFAEGFLAEEKQLHILINNAGVMMCPYSKTADGFETHLGVNHLGHFLLTYL 157
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S ARVV VSS H++G++ DLQS YS AY SKLA VLF+
Sbjct: 158 LLEQLKVSA----PARVVNVSSVAHHIGKIPFHDLQSEKRYSRGFAYCHSKLANVLFTRE 213
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAA 927
L L+ G+ VT PGVV ++L RH LL +LF F +T EGA TS++ A
Sbjct: 214 LAKRLQ--GTGVTTYAVHPGVVRSELVRHSSL-LCLLWRLFS-PFVKTAREGAQTSLHCA 269
Query: 928 VSPALEGVGGRYLYTRRRPSHWRSRTTRS 1014
++ LE + G+Y +R W S R+
Sbjct: 270 LAEGLEPLSGKYFSDCKRT--WVSPRARN 296
>ref|NP_057110.2| androgen-regulated short-chain dehydrogenase/reductase 1 [Homo
sapiens]
Length = 318
Score = 151 bits (381), Expect = 1e-36
Identities = 97/252 (38%), Positives = 136/252 (53%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+V +VTG GIG TAK LA+ G V +A + K ++I+ T N +V
Sbjct: 40 PGKVVVVTGANTGIGKETAKELAQRGARVYLACRDVEKGELVAKEIQTTTGNQQVLVRKL 99
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DL+ KSIR F + F ++ LHVL+NNAGVMM P +T DGFE H GVN+
Sbjct: 100 DLSDTKSIRAFAKGFLAEEKHLHVLINNAGVMMCPYSKTADGFEMHIGVNHLGHFLLTHL 159
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S +R+V VSS H++G + +LQ Y+ AY SKLA +LF+
Sbjct: 160 LLEKLKESA----PSRIVNVSSLAHHLGRIHFHNLQGEKFYNAGLAYCHSKLANILFTQE 215
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAA 927
L L KGS VT PG V ++L RH + R + LF +F +TP +GA TS++ A
Sbjct: 216 LARRL--KGSGVTTYSVHPGTVQSELVRHSSF-MRWMWWLFS-FFIKTPQQGAQTSLHCA 271
Query: 928 VSPALEGVGGRY 963
++ LE + G +
Sbjct: 272 LTEGLEILSGNH 283
>ref|NP_057457.1| WW domain-containing oxidoreductase isoform 1 [Homo sapiens]
Length = 414
Score = 144 bits (363), Expect = 1e-34
Identities = 101/259 (38%), Positives = 133/259 (51%), Gaps = 8/259 (3%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G+V +VTG GIGF TAK A G HVI+A N ++A EAV +I EE KVE + D
Sbjct: 124 GKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWHKAKVEAMTLD 183
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
LA ++S++ F + FK K +PLHVLV NA +P T+DG E F VN+
Sbjct: 184 LALLRSVQHFAEAFKAKNVPLHVLVCNAATFALPWSLTKDGLETTFQVNHLGHFYLVQLL 243
Query: 571 XXXXQASGSPGRSARVVTVSSATH-------YVGELDLDDLQ-SSGGYSPHGAYARSKLA 726
S ARV+ VSS +H +G+LD L + Y AY RSKL
Sbjct: 244 QDVLCRSA----PARVIVVSSESHRFTDINDSLGKLDFSRLSPTKNDYWAMLAYNRSKLC 299
Query: 727 LVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGA 906
+LFS+ L L +G VT+N PG + +W LL L F ++ +GA
Sbjct: 300 NILFSNELHRRLSPRG--VTSNAVHPGNMMYSNIHRSWWVYTLLFTL-ARPFTKSMQQGA 356
Query: 907 WTSVYAAVSPALEGVGGRY 963
T+VY A P LEG+GG Y
Sbjct: 357 ATTVYCAAVPELEGLGGMY 375
>ref|NP_612421.1| retinol dehydrogenase 13 (all-trans and 9-cis) [Homo sapiens]
Length = 260
Score = 132 bits (333), Expect = 4e-31
Identities = 85/220 (38%), Positives = 114/220 (51%), Gaps = 5/220 (2%)
Frame = +1
Query: 319 KAREAVRKIREETLNDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQR 498
K A + IR ETLN V + DLAS+KSIR+F K ++ + +L+NNAGVM P
Sbjct: 3 KCEAAAKDIRGETLNHHVNARHLDLASLKSIREFAAKIIEEEERVDILINNAGVMRCPHW 62
Query: 499 ETEDGFEEHFGVNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQ- 675
TEDGFE FGVN+ +AS +R++ +SS H G +D DDL
Sbjct: 63 TTEDGFEMQFGVNHLGHFLLTNLLLDKLKASA----PSRIINLSSLAHVAGHIDFDDLNW 118
Query: 676 SSGGYSPHGAYARSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHV-FWGTR 852
+ Y+ AY +SKLA+VLF+ L L+ GS VT N PGV T+L RH G+
Sbjct: 119 QTRKYNTKAAYCQSKLAIVLFTKELSRRLQ--GSGVTVNALHPGVARTELGRHTGIHGST 176
Query: 853 LLKKLFG---WWFFQTPDEGAWTSVYAAVSPALEGVGGRY 963
G W ++P+ A S Y AV+ L V G+Y
Sbjct: 177 FSSTTLGPIFWLLVKSPELAAQPSTYLAVAEELADVSGKY 216
>ref|NP_653284.1| hypothetical protein LOC147015 [Homo sapiens]
Length = 327
Score = 107 bits (268), Expect = 1e-23
Identities = 82/238 (34%), Positives = 116/238 (48%), Gaps = 3/238 (1%)
Frame = +1
Query: 259 TAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCDLASMKSIRQFVQKFKM 438
TA LAR G V++A + + A +R+E+ N++V F+ DLAS+ S+R F F
Sbjct: 2 TALELARRGARVVLACRSQERGEAAAFDLRQESGNNEVIFMALDLASLASVRAFATAFLS 61
Query: 439 KKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXXXXXXQASGSPGRSARV 618
+ L +L++NAG+ + T + F VN+ +A +RV
Sbjct: 62 SEPRLDILIHNAGISSCGR--TREAFNLLLRVNHIGPFLLTHLLLPCLKACA----PSRV 115
Query: 619 VTVSSATHYVGELDLDDLQSS--GGYSPHGAYARSKLALVLFSHRLQVLLEAKGSPVTAN 792
V V+SA H G LD L G AYA +KLA VLF+ L LEA G VT
Sbjct: 116 VVVASAAHCRGRLDFKRLDRPVVGWRQELRAYADTKLANVLFARELANQLEATG--VTCY 173
Query: 793 VADPGVVNTDLY-RHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAAVSPALEGVGGRY 963
A PG VN++L+ RHV R L + W + P GA T +Y A+ +E + GRY
Sbjct: 174 AAHPGPVNSELFLRHVPGWLRPLLRPLAWLVLRAPRGGAQTPLYCALQEGIEPLSGRY 231
>ref|XP_371503.3| PREDICTED: similar to short-chain dehydrogenase/reductase 1 [Homo
sapiens]
Length = 891
Score = 71.6 bits (174), Expect = 1e-12
Identities = 40/111 (36%), Positives = 65/111 (58%), Gaps = 1/111 (0%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G++AIVTG GIG ++ LAR G VI+ + ++A+ +I+ + ++++ D
Sbjct: 602 GKIAIVTGANSGIGKVVSQDLARCGAQVILTCQSRECGQQALAEIQAASNSNRLLLGEVD 661
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVM-MVPQRETEDGFEEHFGVNY 540
L+SM SIR F ++ + +H+LVNNAGV M+P+ T G + F NY
Sbjct: 662 LSSMTSIRSFARRLLQENPEIHLLVNNAGVSGMIPKTLTPGGLDLTFVTNY 712
>ref|XP_947127.1| PREDICTED: similar to short-chain dehydrogenase/reductase 1 [Homo
sapiens]
Length = 916
Score = 71.6 bits (174), Expect = 1e-12
Identities = 40/111 (36%), Positives = 65/111 (58%), Gaps = 1/111 (0%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G++AIVTG GIG ++ LAR G VI+ + ++A+ +I+ + ++++ D
Sbjct: 627 GKIAIVTGANSGIGKVVSQDLARCGAQVILTCQSRECGQQALAEIQAASNSNRLLLGEVD 686
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVM-MVPQRETEDGFEEHFGVNY 540
L+SM SIR F ++ + +H+LVNNAGV M+P+ T G + F NY
Sbjct: 687 LSSMTSIRSFARRLLQENPEIHLLVNNAGVSGMIPKTLTPGGLDLTFVTNY 737
>ref|NP_005785.1| dehydrogenase/reductase (SDR family) member 2 isoform 2 [Homo
sapiens]
Length = 280
Score = 61.6 bits (148), Expect = 1e-09
Identities = 64/219 (29%), Positives = 86/219 (39%)
Frame = +1
Query: 214 RVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCDL 393
RVA+VTG T GIGFA A+ LAR G HV+I+ A+ K++ E L+ V + C +
Sbjct: 37 RVAVVTGSTSGIGFAIARRLARDGAHVVISSRKQQNVDRAMAKLQGEGLS--VAGIVCHV 94
Query: 394 ASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXXX 573
+ Q V K + LV +AGV P + G E
Sbjct: 95 GKAEDREQLVAKALEHCGGVDFLVCSAGVN--PLVGSTLGTSEQIWDKILSVNVKSPALL 152
Query: 574 XXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHRLQ 753
R V+ VSS Y + L G Y SK AL+ + L
Sbjct: 153 LSQLLPYMENRRGAVILVSSIAAYNPVVAL------------GVYNVSKTALLGLTRTL- 199
Query: 754 VLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLF 870
LE + N PG++ TD + VF G L K F
Sbjct: 200 -ALELAPKDIRVNCVVPGIIKTD-FSKVFHGNESLWKNF 236
Database: RefSeqHP
Posted date: Aug 2, 2006 12:57 AM
Number of letters in database: 17,774,539
Number of sequences in database: 39,411
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,413,638
Number of Sequences: 39411
Number of extensions: 694281
Number of successful extensions: 3228
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 2819
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3190
length of database: 17,774,539
effective HSP length: 103
effective length of database: 13,715,206
effective search space used: 3209358204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqMP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611C-006582
(1015 letters)
Database: RefSeqMP
45,328 sequences; 21,768,885 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001028498.1| dehydrogenase/reductase (SDR family) X chrom... 319 3e-87
Alignment gi|NP_076186.1| alcohol dehydrogenase PAN2 [Mus musculus] 169 3e-42
Alignment gi|NP_780581.1| retinol dehydrogenase 13 (all-trans and 9-cis) ... 163 3e-40
Alignment gi|NP_084293.1| retinol dehydrogenase 12 [Mus musculus] 160 2e-39
Alignment gi|NP_067532.2| short-chain dehydrogenase/reductase 1 [Mus musc... 156 4e-38
Alignment gi|NP_062519.2| WW-domain oxidoreductase [Mus musculus] 146 3e-35
Alignment gi|NP_899109.1| hypothetical protein LOC70451 [Mus musculus] 101 1e-21
Alignment gi|NP_082066.1| dehydrogenase/reductase member 2 [Mus musculus] 59 6e-09
Alignment gi|NP_766635.1| carbonyl reductase 3 [Mus musculus] 57 2e-08
Alignment gi|XP_487442.1| PREDICTED: similar to Carbonyl reductase [NADPH... 57 3e-08
>ref|NP_001028498.1| dehydrogenase/reductase (SDR family) X chromosome [Mus musculus]
Length = 335
Score = 319 bits (818), Expect = 3e-87
Identities = 158/273 (57%), Positives = 198/273 (72%)
Frame = +1
Query: 148 LAQLLRRCRGGFAEPVFPPQPGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAR 327
LAQLLRR RG F PV PPQPGRVAIVTG T GIG +TA+ LARLGM V++AGN++ + +
Sbjct: 22 LAQLLRRLRGDFRPPVLPPQPGRVAIVTGATAGIGRSTARQLARLGMCVVVAGNDEHRGQ 81
Query: 328 EAVRKIREETLNDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETE 507
E V IR E +D+ FL DLAS+ S+R F + F+ +PLH+LVNNAGVM+ P+ ETE
Sbjct: 82 EVVSSIRAEMGSDRAHFLPLDLASLASVRGFARDFQALGLPLHLLVNNAGVMLEPRAETE 141
Query: 508 DGFEEHFGVNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGG 687
DGFE H GVN+ +ASG+ GR +RVVTV SATHYVG +D+ DL
Sbjct: 142 DGFERHLGVNFLGHFLLTLLLLPALRASGAEGRGSRVVTVGSATHYVGTVDMADLHGRHA 201
Query: 688 YSPHGAYARSKLALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKL 867
YSP+ AYA+SKLAL LF+ +LQ +L+A+G PVT+N+ADPGVV+T+LYRH W R K+
Sbjct: 202 YSPYAAYAQSKLALALFALQLQRILDARGDPVTSNMADPGVVDTELYRHAGWVLRTAKRF 261
Query: 868 FGWWFFQTPDEGAWTSVYAAVSPALEGVGGRYL 966
GW F++P+EGAWT VYAA +P LEGVGGRYL
Sbjct: 262 LGWLVFKSPEEGAWTLVYAAAAPELEGVGGRYL 294
>ref|NP_076186.1| alcohol dehydrogenase PAN2 [Mus musculus]
Length = 334
Score = 169 bits (429), Expect = 3e-42
Identities = 104/263 (39%), Positives = 146/263 (55%), Gaps = 12/263 (4%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETL---------- 360
G+ ++TG G+G ATA L RLG VI+ + ++A EA ++R+E
Sbjct: 44 GKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRQELCQAGGAGPDGT 103
Query: 361 NDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNY 540
+ ++ DLAS++S+R F Q+ ++ L VL+NNAGV P +TEDGFE FGVN+
Sbjct: 104 DGQLVVKELDLASLRSVRAFCQELLQEEPRLDVLINNAGVFHCPYTKTEDGFEMQFGVNH 163
Query: 541 XXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSK 720
++S +R+V VSS + GE++ +DL S Y+ Y+RSK
Sbjct: 164 LGHFLLTNLLLGLLKSSA----PSRIVVVSSKLYKYGEINFEDLNSEQSYNKSFCYSRSK 219
Query: 721 LALVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWG--TRLLKKLFGWWFFQTP 894
LA +LF+ L LE G+ VT NV PG+V T+L RH+ R L L W FF+TP
Sbjct: 220 LANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLARPLFNLVSWAFFKTP 277
Query: 895 DEGAWTSVYAAVSPALEGVGGRY 963
EGA TS+Y A SP +EGV GRY
Sbjct: 278 LEGAQTSIYLACSPDVEGVSGRY 300
>ref|NP_780581.1| retinol dehydrogenase 13 (all-trans and 9-cis) [Mus musculus]
Length = 334
Score = 163 bits (412), Expect = 3e-40
Identities = 114/280 (40%), Positives = 143/280 (51%), Gaps = 11/280 (3%)
Frame = +1
Query: 157 LLRRCRGGFAEPVFPPQPGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAV 336
LL+ G A P PG+ IVTG GIG TA LA+ G +VI+A + K A
Sbjct: 20 LLKDYVAGGACPSKATIPGKTVIVTGANTGIGKQTALELAKRGGNVILACRDMEKCEVAA 79
Query: 337 RKIREETLNDKVEFLYCDLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGF 516
+ IR ETLN +V DLAS+KSIR+F +K ++ + +LVNNA VM P TEDGF
Sbjct: 80 KDIRGETLNPRVRAERLDLASLKSIREFARKVIKEEERVDILVNNAAVMRCPHWTTEDGF 139
Query: 517 EEHFGVNYXXXXXXXXXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQ-SSGGYS 693
E FGVNY +AS +R++ +SS H G +D +DL Y
Sbjct: 140 EMQFGVNYLGHFLLTNLLLDKLKASA----PSRIINLSSLAHVAGHIDFEDLNWQMKKYD 195
Query: 694 PHGAYARSKLALVLF----SHRLQVLLEAKGSPVTANVADPGVVNTDLYRH------VFW 843
AY +SKLA+VLF SHRLQ GS VT N PGV T+L RH F
Sbjct: 196 TKAAYCQSKLAVVLFTKELSHRLQ------GSGVTVNALHPGVARTELGRHTGMHNSAFS 249
Query: 844 GTRLLKKLFGWWFFQTPDEGAWTSVYAAVSPALEGVGGRY 963
G L F W F++P A S Y AV+ LE V G+Y
Sbjct: 250 GFML--GPFFWLLFKSPQLAAQPSTYLAVAEELENVSGKY 287
>ref|NP_084293.1| retinol dehydrogenase 12 [Mus musculus]
Length = 316
Score = 160 bits (406), Expect = 2e-39
Identities = 104/269 (38%), Positives = 147/269 (54%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+V ++TG GIG TA+ LAR G V IA + K A +IR +T N +V
Sbjct: 38 PGKVVVITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKL 97
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DL+ KSIR F ++F ++ LH+L+NNAGVMM P +T DGFE HFGVN+
Sbjct: 98 DLSDTKSIRAFAERFLAEEKKLHILINNAGVMMCPYSKTTDGFETHFGVNHLGHFLLTYL 157
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S ARVV +SS H +G++ DLQ Y AY SKLA +LF+
Sbjct: 158 LLERLKESA----PARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLFTRE 213
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAA 927
L L+ G+ VTA PGVV +++ R+ + LL +LF FF++ +GA TS++ A
Sbjct: 214 LAKRLQ--GTGVTAYAVHPGVVLSEITRNSYL-LCLLWRLFS-PFFKSTSQGAQTSLHCA 269
Query: 928 VSPALEGVGGRYLYTRRRPSHWRSRTTRS 1014
++ LE + G+Y +R W S R+
Sbjct: 270 LAEDLEPLSGKYFSDCKR--MWVSSRARN 296
>ref|NP_067532.2| short-chain dehydrogenase/reductase 1 [Mus musculus]
Length = 316
Score = 156 bits (394), Expect = 4e-38
Identities = 103/252 (40%), Positives = 139/252 (55%)
Frame = +1
Query: 208 PGRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYC 387
PG+VAIVTG GIG TAK LA+ G V +A + K A R+I+ T N +V
Sbjct: 37 PGKVAIVTGANTGIGKETAKDLAQRGARVYLACRDVDKGELAAREIQAVTGNSQVFVRKL 96
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
DLA KSIR F + F ++ LH+L+NNAGVMM P +T DGFE H GVN+
Sbjct: 97 DLADTKSIRAFAKDFLAEEKHLHLLINNAGVMMCPYSKTADGFEMHIGVNHLGHFLLTHL 156
Query: 568 XXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFSHR 747
+ S +R+V +SS H++G + +LQ YS AY SKLA +LF+
Sbjct: 157 LLEKLKESA----PSRIVNLSSLGHHLGRIHFHNLQGEKFYSAGLAYCHSKLANILFTKE 212
Query: 748 LQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGAWTSVYAA 927
L L KGS VT PG V+++L R+ R L +LF + F +TP EGA TS+Y A
Sbjct: 213 LAKRL--KGSGVTTYSVHPGTVHSELTRYSSI-MRWLWQLF-FVFIKTPQEGAQTSLYCA 268
Query: 928 VSPALEGVGGRY 963
++ LE + G +
Sbjct: 269 LTEGLESLSGSH 280
>ref|NP_062519.2| WW-domain oxidoreductase [Mus musculus]
Length = 414
Score = 146 bits (369), Expect = 3e-35
Identities = 106/259 (40%), Positives = 136/259 (52%), Gaps = 8/259 (3%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G+V +VTG GIGF TAK A G HVI+A N S+A EAV +I EE KVE + D
Sbjct: 124 GKVVLVTGANSGIGFETAKSFALHGAHVILACRNLSRASEAVSRILEEWHKAKVEAMTLD 183
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
LA ++S++ F + FK K + LHVLV NAG +P T+DG E F VN+
Sbjct: 184 LAVLRSVQHFAEAFKAKNVSLHVLVCNAGTFALPWGLTKDGLETTFQVNHLGHFYLVQLL 243
Query: 571 XXXXQASGSPGRSARVVTVSSATHYV-------GELDLDDLQ-SSGGYSPHGAYARSKLA 726
S SP ARV+ VSS +H G+LDL L Y AY RSKL
Sbjct: 244 QDVLCRS-SP---ARVIVVSSESHRFTDINDSSGKLDLSRLSPPRSDYWAMLAYNRSKLC 299
Query: 727 LVLFSHRLQVLLEAKGSPVTANVADPGVVNTDLYRHVFWGTRLLKKLFGWWFFQTPDEGA 906
+LFS+ L L +G VT+N PG + W +LL L F ++ +GA
Sbjct: 300 NILFSNELHRRLSPRG--VTSNAVHPGNMMYSAIHRNSWVYKLLFTL-ARPFTKSMQQGA 356
Query: 907 WTSVYAAVSPALEGVGGRY 963
T+VY AV+P LEG+GG Y
Sbjct: 357 ATTVYCAVAPELEGLGGMY 375
>ref|NP_899109.1| hypothetical protein LOC70451 [Mus musculus]
Length = 260
Score = 101 bits (252), Expect = 1e-21
Identities = 78/225 (34%), Positives = 110/225 (48%), Gaps = 3/225 (1%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
GR +VTG GIG TA LAR G V++A + + A +R+E+ N++V F+ D
Sbjct: 36 GRTVVVTGANSGIGKMTALELARRGARVVLACRSRERGEAAAFDLRQESGNNEVIFMALD 95
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
LAS+ S++ F F + L VL++NAG+ + T + F VN+
Sbjct: 96 LASLASVQAFATAFLSSEPRLDVLIHNAGISSCGR--TRETFNLLLRVNHVGPFLLTHLL 153
Query: 571 XXXXQASGSPGRSARVVTVSSATHYVGELDLD--DLQSSGGYSPHGAYARSKLALVLFSH 744
++ +RVV VSSA H G LD D G AYA SKLA VLF+
Sbjct: 154 LPRLRSCA----PSRVVIVSSAAHRRGRLDFTRLDCPVVGWQQELRAYADSKLANVLFAR 209
Query: 745 RLQVLLEAKGSPVTANVADPGVVNTDLY-RHVFWGTRLLKKLFGW 876
L LE G+ VT A PG VN++L+ RH+ R + + W
Sbjct: 210 ELATQLE--GTGVTCYAAHPGPVNSELFLRHLRGWLRPILRPLAW 252
>ref|NP_082066.1| dehydrogenase/reductase member 2 [Mus musculus]
Length = 282
Score = 59.3 bits (142), Expect = 6e-09
Identities = 60/207 (28%), Positives = 84/207 (40%), Gaps = 3/207 (1%)
Frame = +1
Query: 211 GRVAIVTGGTEGIGFATAKHLARLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
G+VA++TG T GIGFA A+ LA+ G HV+I+ EAV ++EE L+ V C
Sbjct: 37 GKVAVITGSTRGIGFAIARRLAQDGAHVVISSRKQENVDEAVTILKEEGLS--VTGTMCH 94
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMV---PQRETEDGFEEHFGVNYXXXXXXX 561
+ + + V + LV AGV + +E +++ VN
Sbjct: 95 VGKAEDRQHLVTTALKHSGGIDFLVCVAGVNPLVGSTLGASEQIWDKILDVNVKSPALLL 154
Query: 562 XXXXXXXQASGSPGRSARVVTVSSATHYVGELDLDDLQSSGGYSPHGAYARSKLALVLFS 741
+ R VV VSS YV L G Y SK AL+
Sbjct: 155 SKVLPYMENR----RGGSVVLVSSGVAYVPVPKL------------GVYNTSKTALLGLC 198
Query: 742 HRLQVLLEAKGSPVTANVADPGVVNTD 822
L V L KG + N PG++ TD
Sbjct: 199 KSLAVELAPKG--IRVNCLVPGIIKTD 223
>ref|NP_766635.1| carbonyl reductase 3 [Mus musculus]
Length = 277
Score = 57.4 bits (137), Expect = 2e-08
Identities = 73/274 (26%), Positives = 111/274 (40%), Gaps = 33/274 (12%)
Frame = +1
Query: 214 RVAIVTGGTEGIGFATAKHLAR-LGMHVIIAGNNDSKAREAVRKIREETLNDKVEFLYCD 390
RVA+VTG +GIGFA + L R V++ ++++ R AV++++ E L+ + F D
Sbjct: 6 RVALVTGANKGIGFAITRDLCRKFSGDVVLTARDEARGRAAVQQLQAEGLSPR--FHQLD 63
Query: 391 LASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXXX 570
+ +SIR + + L+VLVNNAG+ + + F+ V
Sbjct: 64 IDDPQSIRALRDFLRKEYGGLNVLVNNAGIAF--RMDDPTPFDIQAEVTLKTNFFATRNV 121
Query: 571 XXXXQASGSPGRSARVVTVSS------------------ATHYVGELDLDDLQSS----- 681
P RVV +SS + E+DL DL
Sbjct: 122 CTELLPIMKP--HGRVVNISSLQGLKALENCREDLQEKFRCDTLTEVDLVDLMKKFVEDT 179
Query: 682 -------GGYSPHGAYARSKLALVLFSHRL--QVLLEAKGSPVTANVADPGVVNTDLYRH 834
G+ P AY SKL + + + L Q+ + K + N PG V TD+ R
Sbjct: 180 KNEVHEREGW-PDSAYGVSKLGVTVLTRILARQLDEKRKADRILLNACCPGWVKTDMARD 238
Query: 835 VFWGTRLLKKLFGWWFFQTPDEGAWTSVYAAVSP 936
G+R T +EGA T VY A+ P
Sbjct: 239 --QGSR------------TVEEGAETPVYLALLP 258
>ref|XP_487442.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) [Mus musculus]
Length = 277
Score = 57.0 bits (136), Expect = 3e-08
Identities = 75/285 (26%), Positives = 117/285 (41%), Gaps = 34/285 (11%)
Frame = +1
Query: 214 RVAIVTGGTEGIGFATAKHLA-RLGMHVIIAGNNDSKAREAVRKIREETLNDKVEFL-YC 387
RVA+VTGG +GIGF + L + V++ ++++ AV+K++ E LN + L
Sbjct: 6 RVALVTGGNKGIGFEIIRDLCQKFSGDVVLTARDEARGCAAVQKLQAEGLNPRFHQLDID 65
Query: 388 DLASMKSIRQFVQKFKMKKIPLHVLVNNAGVMMVPQRETEDGFEEHFGVNYXXXXXXXXX 567
D S+ ++R F+ K + L VLVNNAG+ + +D H
Sbjct: 66 DPQSIHALRDFLLK---EYKGLDVLVNNAGIAF----KVDDPTPFHIKGEVTMKTNFFGT 118
Query: 568 XXXXXQASGSPGRSARVVTVSS------------------ATHYVGELDLDDLQS----- 678
+ RVV VSS + + E +L L +
Sbjct: 119 RDVCKEMLPLIKPQGRVVNVSSMVSLSALKDCSPELQQKFRSETITEEELVGLMNKYVED 178
Query: 679 --SGGYS----PHGAYARSKLALVLFSHRLQVLL--EAKGSPVTANVADPGVVNTDLYRH 834
G Y P+ AY SK+ + + S L L + +G + N PG V TD+
Sbjct: 179 AKKGVYVKEDWPNSAYEVSKIGVTVLSRILARKLNEQRRGDKILLNACCPGWVRTDM--- 235
Query: 835 VFWGTRLLKKLFGWWFFQTPDEGAWTSVY-AAVSPALEGVGGRYL 966
G + K +P+EGA T VY A + P EG G+++
Sbjct: 236 --TGPQATK---------SPEEGAETPVYLALLPPDAEGPHGQFV 269
Database: RefSeqMP
Posted date: Aug 2, 2006 12:58 AM
Number of letters in database: 21,768,885
Number of sequences in database: 45,328
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,458,901
Number of Sequences: 45328
Number of extensions: 777291
Number of successful extensions: 3469
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 3088
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3432
length of database: 21,768,885
effective HSP length: 104
effective length of database: 17,054,773
effective search space used: 3973762109
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)