BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-012449
(1006 letters)
Database: RefSeqSP
1040 sequences; 434,620 total letters
Searching...done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_999143.1| beta 2-microglobulin [Sus scrofa] 120 5e-29
>ref|NP_999143.1| beta 2-microglobulin [Sus scrofa]
Length = 118
Score = 120 bits (301), Expect = 5e-29
Identities = 57/92 (61%), Positives = 67/92 (72%)
Frame = +2
Query: 95 SRPPKVQVYSTHPSDNGKPNYMKCYVTGFHPPQIEIDLMKNRQNDELRSFSLCLSATSGH 274
+RPPKVQVYS HP++NGKPNY+ CYV+GFHPPQIEIDL+KN + L S
Sbjct: 22 ARPPKVQVYSRHPAENGKPNYLNCYVSGFHPPQIEIDLLKNGEKMNAEQSDLSFS-KDWS 80
Query: 275 FYLLVHTDFTPKSVHQ*RCPVKHVTLHKPNIL 370
FYLLVHT+FTP +V Q C VKHVTL KP I+
Sbjct: 81 FYLLVHTEFTPNAVDQYSCRVKHVTLDKPKIV 112
Database: RefSeqSP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 434,620
Number of sequences in database: 1040
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 936,025
Number of Sequences: 1040
Number of extensions: 22654
Number of successful extensions: 70
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 66
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69
length of database: 434,620
effective HSP length: 77
effective length of database: 354,540
effective search space used: 91116780
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqBP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-012449
(1006 letters)
Database: RefSeqBP
33,508 sequences; 16,112,626 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_776318.1| beta-2-microglobulin [Bos taurus] 109 4e-24
>ref|NP_776318.1| beta-2-microglobulin [Bos taurus]
Length = 118
Score = 109 bits (272), Expect = 4e-24
Identities = 52/91 (57%), Positives = 62/91 (68%)
Frame = +2
Query: 98 RPPKVQVYSTHPSDNGKPNYMKCYVTGFHPPQIEIDLMKNRQNDELRSFSLCLSATSGHF 277
RPPK+QVYS HP ++GKPNY+ CYV GFHPPQIEIDL+KN + + L S F
Sbjct: 23 RPPKIQVYSRHPPEDGKPNYLNCYVYGFHPPQIEIDLLKNGEKIKSEQSDLSFS-KDWSF 81
Query: 278 YLLVHTDFTPKSVHQ*RCPVKHVTLHKPNIL 370
YLL H +FTP S Q C VKHVTL +P I+
Sbjct: 82 YLLSHAEFTPNSKDQYSCRVKHVTLEQPRIV 112
Database: RefSeqBP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 16,112,626
Number of sequences in database: 33,508
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,900,686
Number of Sequences: 33508
Number of extensions: 824268
Number of successful extensions: 2310
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 2145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2307
length of database: 16,112,626
effective HSP length: 102
effective length of database: 12,694,810
effective search space used: 2945195920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqHP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-012449
(1006 letters)
Database: RefSeqHP
39,411 sequences; 17,774,539 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_004039.1| beta-2-microglobulin precursor [Homo sapiens] 96 5e-20
>ref|NP_004039.1| beta-2-microglobulin precursor [Homo sapiens]
Length = 119
Score = 95.9 bits (237), Expect = 5e-20
Identities = 44/91 (48%), Positives = 59/91 (64%)
Frame = +2
Query: 98 RPPKVQVYSTHPSDNGKPNYMKCYVTGFHPPQIEIDLMKNRQNDELRSFSLCLSATSGHF 277
R PK+QVYS HP++NGK N++ CYV+GFHP IE+DL+KN + E S + F
Sbjct: 23 RTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSF 82
Query: 278 YLLVHTDFTPKSVHQ*RCPVKHVTLHKPNIL 370
YLL +T+FTP + C V HVTL +P I+
Sbjct: 83 YLLYYTEFTPTEKDEYACRVNHVTLSQPKIV 113
Database: RefSeqHP
Posted date: Aug 2, 2006 12:57 AM
Number of letters in database: 17,774,539
Number of sequences in database: 39,411
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,085,818
Number of Sequences: 39411
Number of extensions: 941534
Number of successful extensions: 2654
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 2410
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2652
length of database: 17,774,539
effective HSP length: 102
effective length of database: 13,754,617
effective search space used: 3191071144
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqCP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-012449
(1006 letters)
Database: RefSeqCP
33,732 sequences; 19,266,565 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_535458.1| PREDICTED: similar to beta-2-microglobulin prec... 109 5e-24
Alignment gi|XP_850148.1| PREDICTED: similar to beta-2-microglobulin prec... 109 5e-24
>ref|XP_535458.1| PREDICTED: similar to beta-2-microglobulin precursor isoform 2
[Canis familiaris]
Length = 125
Score = 109 bits (272), Expect = 5e-24
Identities = 50/90 (55%), Positives = 64/90 (71%)
Frame = +2
Query: 101 PPKVQVYSTHPSDNGKPNYMKCYVTGFHPPQIEIDLMKNRQNDELRSFSLCLSATSGHFY 280
PPK+QVYS HP++NGKPN++ CYV+GFHPP+IEIDL+KN + + L S FY
Sbjct: 31 PPKIQVYSRHPAENGKPNFLNCYVSGFHPPEIEIDLLKNGKEMKAEQTDLSFS-KDWTFY 89
Query: 281 LLVHTDFTPKSVHQ*RCPVKHVTLHKPNIL 370
LLVHT+FTP + C VKHVTL +P I+
Sbjct: 90 LLVHTEFTPNEQDEFSCRVKHVTLSEPQIV 119
>ref|XP_850148.1| PREDICTED: similar to beta-2-microglobulin precursor isoform 3
[Canis familiaris]
Length = 125
Score = 109 bits (272), Expect = 5e-24
Identities = 50/90 (55%), Positives = 64/90 (71%)
Frame = +2
Query: 101 PPKVQVYSTHPSDNGKPNYMKCYVTGFHPPQIEIDLMKNRQNDELRSFSLCLSATSGHFY 280
PPK+QVYS HP++NGKPN++ CYV+GFHPP+IEIDL+KN + + L S FY
Sbjct: 31 PPKIQVYSRHPAENGKPNFLNCYVSGFHPPEIEIDLLKNGKEMKAEQTDLSFS-KDWTFY 89
Query: 281 LLVHTDFTPKSVHQ*RCPVKHVTLHKPNIL 370
LLVHT+FTP + C VKHVTL +P I+
Sbjct: 90 LLVHTEFTPNEQDEFSCRVKHVTLSEPQIV 119
Database: RefSeqCP
Posted date: Aug 1, 2006 9:25 PM
Number of letters in database: 19,266,565
Number of sequences in database: 33,732
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,850,147
Number of Sequences: 33732
Number of extensions: 962240
Number of successful extensions: 2422
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 2220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2417
length of database: 19,266,565
effective HSP length: 103
effective length of database: 15,792,169
effective search space used: 3647991039
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqMP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-012449
(1006 letters)
Database: RefSeqMP
45,328 sequences; 21,768,885 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_033865.2| beta-2-microglobulin [Mus musculus] 88 2e-17
>ref|NP_033865.2| beta-2-microglobulin [Mus musculus]
Length = 119
Score = 87.8 bits (216), Expect = 2e-17
Identities = 39/88 (44%), Positives = 53/88 (60%)
Frame = +2
Query: 98 RPPKVQVYSTHPSDNGKPNYMKCYVTGFHPPQIEIDLMKNRQNDELRSFSLCLSATSGHF 277
+ P++QVYS HP +NGKPN + CYVT FHPP IEI ++KN + S + F
Sbjct: 23 KTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSF 82
Query: 278 YLLVHTDFTPKSVHQ*RCPVKHVTLHKP 361
Y+L HT+FTP C VKH ++ +P
Sbjct: 83 YILAHTEFTPTETDTYACRVKHASMAEP 110
Database: RefSeqMP
Posted date: Aug 2, 2006 12:58 AM
Number of letters in database: 21,768,885
Number of sequences in database: 45,328
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,902,719
Number of Sequences: 45328
Number of extensions: 1122083
Number of successful extensions: 3044
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 2800
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3043
length of database: 21,768,885
effective HSP length: 104
effective length of database: 17,054,773
effective search space used: 3922597790
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)