BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-013008
(1188 letters)
Database: RefSeqSP
1040 sequences; 434,620 total letters
Searching...done
***** No hits found ******
Database: RefSeqSP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 434,620
Number of sequences in database: 1040
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,178,428
Number of Sequences: 1040
Number of extensions: 32410
Number of successful extensions: 106
Number of sequences better than 1.0e-05: 0
Number of HSP's better than 0.0 without gapping: 90
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 434,620
effective HSP length: 79
effective length of database: 352,460
effective search space used: 111377360
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqBP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-013008
(1188 letters)
Database: RefSeqBP
33,508 sequences; 16,112,626 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_584353.2| PREDICTED: similar to ubiquitin-conjugating enz... 456 e-128
>ref|XP_584353.2| PREDICTED: similar to ubiquitin-conjugating enzyme variant Kua [Bos
taurus]
Length = 271
Score = 456 bits (1172), Expect = e-128
Identities = 216/271 (79%), Positives = 224/271 (82%), Gaps = 40/271 (14%)
Frame = +1
Query: 106 MAGAEDGPGQQLELDEDEAAYCRRWGAQHAGARELAALYSP------------------- 228
MAGAEDGPGQQ EL++DEAA CRRWGAQHAGARELAALYSP
Sbjct: 1 MAGAEDGPGQQPELEDDEAASCRRWGAQHAGARELAALYSPGKRFQEWCCVVLCFSLIAH 60
Query: 229 ---------------------VAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE 345
VAGAL+ADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE
Sbjct: 61 NMAHLLLLARWEHTPLVMLGMVAGALLADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE 120
Query: 346 HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPEALEQLYPWECFIFCLVIFV 525
HHIDPTAITRHDFIETNGDNCL+TLLPLLNMAYKFRTQSPE LEQLYPWECF+FCL+IF
Sbjct: 121 HHIDPTAITRHDFIETNGDNCLLTLLPLLNMAYKFRTQSPEVLEQLYPWECFVFCLIIFG 180
Query: 526 TFTNQIHKWSHTYFGLPSWVILLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEK 705
TFTNQIHKWSHTYFGLP WV+ LQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLE+
Sbjct: 181 TFTNQIHKWSHTYFGLPCWVVFLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLER 240
Query: 706 MGFWRRLEDIIQGLTGEKPRADDMKWAQKIK 798
MGFWRRLEDIIQ LTGEKPRADDMKWAQKIK
Sbjct: 241 MGFWRRLEDIIQALTGEKPRADDMKWAQKIK 271
Database: RefSeqBP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 16,112,626
Number of sequences in database: 33,508
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,875,095
Number of Sequences: 33508
Number of extensions: 1304485
Number of successful extensions: 5084
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 4200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5075
length of database: 16,112,626
effective HSP length: 104
effective length of database: 12,627,794
effective search space used: 3674688054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqHP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-013008
(1188 letters)
Database: RefSeqHP
39,411 sequences; 17,774,539 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_954580.1| ubiquitin-conjugating enzyme E2 variant 1 [Homo... 453 e-127
Alignment gi|NP_954673.1| ubiquitin-conjugating enzyme E2 Kua-UEV isoform... 372 e-103
Alignment gi|NP_003340.1| ubiquitin-conjugating enzyme E2 Kua-UEV isoform... 179 5e-45
>ref|NP_954580.1| ubiquitin-conjugating enzyme E2 variant 1 [Homo sapiens]
Length = 270
Score = 453 bits (1165), Expect = e-127
Identities = 219/271 (80%), Positives = 224/271 (82%), Gaps = 40/271 (14%)
Frame = +1
Query: 106 MAGAEDGPGQQLELDEDEAAYCRRWGAQHAGARELAALYSP------------------- 228
MAGAED PGQQLELDEDEA+ CR WGAQHAGARELAALYSP
Sbjct: 1 MAGAEDWPGQQLELDEDEASCCR-WGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAH 59
Query: 229 ---------------------VAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE 345
VAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE
Sbjct: 60 NLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE 119
Query: 346 HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPEALEQLYPWECFIFCLVIFV 525
HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRT SPEALEQLYPWECF+FCL+IF
Sbjct: 120 HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEALEQLYPWECFVFCLIIFG 179
Query: 526 TFTNQIHKWSHTYFGLPSWVILLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEK 705
TFTNQIHKWSHTYFGLP WV LLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEK
Sbjct: 180 TFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEK 239
Query: 706 MGFWRRLEDIIQGLTGEKPRADDMKWAQKIK 798
+GFWRRLED+IQGLTGEKPRADDMKWAQKIK
Sbjct: 240 IGFWRRLEDLIQGLTGEKPRADDMKWAQKIK 270
>ref|NP_954673.1| ubiquitin-conjugating enzyme E2 Kua-UEV isoform 1 [Homo sapiens]
Length = 370
Score = 372 bits (956), Expect = e-103
Identities = 188/256 (73%), Positives = 196/256 (76%), Gaps = 40/256 (15%)
Frame = +1
Query: 106 MAGAEDGPGQQLELDEDEAAYCRRWGAQHAGARELAALYSP------------------- 228
MAGAED PGQQLELDEDEA+ CR WGAQHAGARELAALYSP
Sbjct: 1 MAGAEDWPGQQLELDEDEASCCR-WGAQHAGARELAALYSPGKRLQEWCSVILCFSLIAH 59
Query: 229 ---------------------VAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE 345
VAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE
Sbjct: 60 NLVHLLLLARWEDTPLVILGVVAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE 119
Query: 346 HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPEALEQLYPWECFIFCLVIFV 525
HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRT SPEALEQLYPWECF+FCL+IF
Sbjct: 120 HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTHSPEALEQLYPWECFVFCLIIFG 179
Query: 526 TFTNQIHKWSHTYFGLPSWVILLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEK 705
TFTNQIHKWSHTYFGLP WV LLQDWHVILPRKHHRIHHVSPHETYFCITTG + P
Sbjct: 180 TFTNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTG-VKVPRN- 237
Query: 706 MGFWRRLEDIIQGLTG 753
+R LE++ +G G
Sbjct: 238 ---FRLLEELEEGQKG 250
>ref|NP_003340.1| ubiquitin-conjugating enzyme E2 Kua-UEV isoform 2 [Homo sapiens]
Length = 221
Score = 179 bits (453), Expect = 5e-45
Identities = 82/106 (77%), Positives = 89/106 (83%)
Frame = +1
Query: 436 MAYKFRTQSPEALEQLYPWECFIFCLVIFVTFTNQIHKWSHTYFGLPSWVILLQDWHVIL 615
MAYKFRT SPEALEQLYPWECF+FCL+IF TFTNQIHKWSHTYFGLP WV LLQDWHVIL
Sbjct: 1 MAYKFRTHSPEALEQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHVIL 60
Query: 616 PRKHHRIHHVSPHETYFCITTGWLNYPLEKMGFWRRLEDIIQGLTG 753
PRKHHRIHHVSPHETYFCITTG + P +R LE++ +G G
Sbjct: 61 PRKHHRIHHVSPHETYFCITTG-VKVPRN----FRLLEELEEGQKG 101
Database: RefSeqHP
Posted date: Aug 2, 2006 12:57 AM
Number of letters in database: 17,774,539
Number of sequences in database: 39,411
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,386,040
Number of Sequences: 39411
Number of extensions: 1556994
Number of successful extensions: 6056
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 4814
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6042
length of database: 17,774,539
effective HSP length: 104
effective length of database: 13,675,795
effective search space used: 3979656345
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqCP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-013008
(1188 letters)
Database: RefSeqCP
33,732 sequences; 19,266,565 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_854159.1| PREDICTED: similar to ubiquitin-conjugating enz... 261 1e-69
>ref|XP_854159.1| PREDICTED: similar to ubiquitin-conjugating enzyme E2 variant 1
[Canis familiaris]
Length = 121
Score = 261 bits (666), Expect = 1e-69
Identities = 113/121 (93%), Positives = 118/121 (97%)
Frame = +1
Query: 436 MAYKFRTQSPEALEQLYPWECFIFCLVIFVTFTNQIHKWSHTYFGLPSWVILLQDWHVIL 615
MAYKFRTQSPEALEQLYPWECF+FCL+IF TFTNQIHKWSHTYFGLP WV LLQDWH+IL
Sbjct: 1 MAYKFRTQSPEALEQLYPWECFVFCLIIFGTFTNQIHKWSHTYFGLPRWVTLLQDWHIIL 60
Query: 616 PRKHHRIHHVSPHETYFCITTGWLNYPLEKMGFWRRLEDIIQGLTGEKPRADDMKWAQKI 795
PRKHHRIHHVSPHETYFCITTGWLNYPLEK+GFWRRLED+IQGLTGEKPRADDMKWAQKI
Sbjct: 61 PRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQKI 120
Query: 796 K 798
K
Sbjct: 121 K 121
Database: RefSeqCP
Posted date: Aug 1, 2006 9:25 PM
Number of letters in database: 19,266,565
Number of sequences in database: 33,732
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,498,980
Number of Sequences: 33732
Number of extensions: 1528843
Number of successful extensions: 5868
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 4743
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5861
length of database: 19,266,565
effective HSP length: 105
effective length of database: 15,724,705
effective search space used: 4560164450
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqMP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-013008
(1188 letters)
Database: RefSeqMP
45,328 sequences; 21,768,885 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_663513.1| ubiquitin-conjugating enzyme variant Kua [Mus m... 441 e-124
Alignment gi|XP_980742.1| PREDICTED: similar to ubiquitin-conjugating enz... 191 4e-61
>ref|NP_663513.1| ubiquitin-conjugating enzyme variant Kua [Mus musculus]
Length = 271
Score = 441 bits (1134), Expect = e-124
Identities = 211/271 (77%), Positives = 219/271 (80%), Gaps = 40/271 (14%)
Frame = +1
Query: 106 MAGAEDGPGQQLELDEDEAAYCRRWGAQHAGARELAALYSP------------------- 228
MAGAED PG+Q ELDEDE A RRWGAQHAGARELAALYSP
Sbjct: 1 MAGAEDAPGRQPELDEDETAEGRRWGAQHAGARELAALYSPGKRFQEWCSVILCFSLIAH 60
Query: 229 ---------------------VAGALIADFLSGLVHWGADTWGSVELPIVGKAFIRPFRE 345
VAGAL+ADFLSGLVHWGADTWGSV+LPIVGKAFIRPFRE
Sbjct: 61 NLVHLLLLARWEHTPLVILGVVAGALVADFLSGLVHWGADTWGSVDLPIVGKAFIRPFRE 120
Query: 346 HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPEALEQLYPWECFIFCLVIFV 525
HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPE LEQLYPWECF+FCL IF
Sbjct: 121 HHIDPTAITRHDFIETNGDNCLVTLLPLLNMAYKFRTQSPETLEQLYPWECFVFCLTIFG 180
Query: 526 TFTNQIHKWSHTYFGLPSWVILLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEK 705
TFTNQIHKWSHTY GLP WV +LQDWHVILPRKHHRIHHV+PHETYFCITTGWLNYPLE
Sbjct: 181 TFTNQIHKWSHTYLGLPYWVTVLQDWHVILPRKHHRIHHVAPHETYFCITTGWLNYPLEV 240
Query: 706 MGFWRRLEDIIQGLTGEKPRADDMKWAQKIK 798
+GFWRRLED+IQGLTGEKPRADDMKWAQKIK
Sbjct: 241 IGFWRRLEDLIQGLTGEKPRADDMKWAQKIK 271
>ref|XP_980742.1| PREDICTED: similar to ubiquitin-conjugating enzyme variant Kua [Mus
musculus]
Length = 270
Score = 191 bits (485), Expect(2) = 4e-61
Identities = 113/202 (55%), Positives = 117/202 (57%), Gaps = 7/202 (3%)
Frame = +2
Query: 209 WPR-STH------QLQGRSLLTSCLAWCTGEPTPGAPWNCPLWGRLSSDPSESITSTQQP 367
WP STH LQG S LTSC AW G GA W CPL GR SS S S T T QP
Sbjct: 67 WPAGSTHPSXSWVSLQGLSSLTSCPAWYIGVLIRGALWICPLLGRPSSGRSGSTTLTPQP 126
Query: 368 LQGMTSSRPMGTTAW*RSCHC*TWPTSSAPRAQKPWSSCTPGNAXXXXXXXXXXXXXXXX 547
QG TSSRPM TTAW R CHC TWPTSS P AQ+PWSSCT GNA
Sbjct: 127 SQGTTSSRPMRTTAWSRCCHCXTWPTSSGPIAQRPWSSCTLGNASSSVXPSLAPSPTRST 186
Query: 548 XGRTHTLGCRAGSSSCRTGMSSCPVNIIASTMSHPTRPTSASPQAGSTTLWRRWASGDA* 727
G THT GC AGS SCRTG S N AST PT+ S S QAGSTT R ASG A
Sbjct: 187 SGHTHTXGCHAGSLSCRTGTXSYHGNTDASTTWRPTKHPSVSLQAGSTTAQRLXASGGAW 246
Query: 728 KTSSKA*QARSLGRTT*NGHRR 793
+ SSK + RS RTT NG RR
Sbjct: 247 RISSKVXRVRSRERTTXNGPRR 268
Score = 63.5 bits (153), Expect(2) = 4e-61
Identities = 31/41 (75%), Positives = 33/41 (80%)
Frame = +1
Query: 106 MAGAEDGPGQQLELDEDEAAYCRRWGAQHAGARELAALYSP 228
MAGAED G+Q ELDED+ A RRWG QH GARELAALYSP
Sbjct: 1 MAGAEDTLGRQPELDEDKKAEGRRWGTQHTGARELAALYSP 41
Database: RefSeqMP
Posted date: Aug 2, 2006 12:58 AM
Number of letters in database: 21,768,885
Number of sequences in database: 45,328
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,709,822
Number of Sequences: 45328
Number of extensions: 1711732
Number of successful extensions: 6650
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 5330
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6611
length of database: 21,768,885
effective HSP length: 106
effective length of database: 16,964,117
effective search space used: 4902629813
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)