BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-057194
(1386 letters)
Database: RefSeqSP
1040 sequences; 434,620 total letters
Searching...done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_999238.1| 20-beta-hydroxysteroid dehydrogenase [Sus scrofa] 250 5e-68
>ref|NP_999238.1| 20-beta-hydroxysteroid dehydrogenase [Sus scrofa]
Length = 289
Score = 250 bits (639), Expect = 5e-68
Identities = 127/181 (70%), Positives = 149/181 (82%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKGIGFA++ DLC+QFAGD++LT +DVA G + QLQA+GL+P F LDII+
Sbjct: 7 VALVTGANKGIGFAIVRDLCRQFAGDVVLTARDVARGQAAVKQLQAEGLSPRFHQLDIID 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SIRA+ DFLR EYGGLDVLVNNAAIA+QLDNPTP HI A LTMKTNF+GT NVCT LL
Sbjct: 67 LQSIRALCDFLRKEYGGLDVLVNNAAIAFQLDNPTPFHIQAELTMKTNFMGTRNVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PLIKPQG VV VSS E VRAL++C PELQQ F ET+TE+ELVGL+ F++D+K RK
Sbjct: 127 PLIKPQGRVVNVSSTEGVRALNECSPELQQKFKSETITEEELVGLMNKFVEDTKNGVHRK 186
Query: 667 D 669
+
Sbjct: 187 E 187
Score = 51.2 bits (121), Expect = 6e-08
Identities = 32/90 (35%), Positives = 46/90 (51%)
Frame = +3
Query: 615 GALEKFFARLKNKVHKKR*WIDSTLLMTKIGVPVIVKDLAQEPQISNNMEKLST*IPYCP 794
G + KF KN VH+K W DST +TKIGV V+ + A++ + +K+ CP
Sbjct: 170 GLMNKFVEDTKNGVHRKEGWSDSTYGVTKIGVSVLSRIYARKLREQRAGDKILL-NACCP 228
Query: 795 WWIDNQHWGNPMPPVSQNNKRKTPLFLVLL 884
W+ G P P S +TP++L LL
Sbjct: 229 GWV-RTDMGGPKAPKSPEVGAETPVYLALL 257
Database: RefSeqSP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 434,620
Number of sequences in database: 1040
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,325,659
Number of Sequences: 1040
Number of extensions: 35944
Number of successful extensions: 101
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 97
length of database: 434,620
effective HSP length: 80
effective length of database: 351,420
effective search space used: 133891020
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqBP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-057194
(1386 letters)
Database: RefSeqBP
33,508 sequences; 16,112,626 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001030258.1| 20-beta-hydroxysteroid dehydrogenase-like [B... 216 3e-56
Alignment gi|NP_001029685.1| carbonyl reductase 1 [Bos taurus] 214 1e-55
Alignment gi|XP_869745.1| PREDICTED: similar to carbonyl reductase 3 isof... 205 6e-53
Alignment gi|XP_874747.1| PREDICTED: similar to Carbonyl reductase [NADPH... 205 6e-53
Alignment gi|XP_868956.1| PREDICTED: similar to Carbonyl reductase [NADPH... 100 4e-21
Alignment gi|XP_878838.1| PREDICTED: similar to carbonyl reductase 3 isof... 83 6e-16
Alignment gi|XP_594165.2| PREDICTED: similar to carbonyl reductase 3 isof... 83 6e-16
>ref|NP_001030258.1| 20-beta-hydroxysteroid dehydrogenase-like [Bos taurus]
Length = 286
Score = 216 bits (551), Expect = 3e-56
Identities = 109/181 (60%), Positives = 137/181 (75%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKG+GFA++ DLC++F GD++LT +D A G + QLQA+GL+P F LDI +
Sbjct: 7 VALVTGANKGLGFAIVRDLCRRFPGDVVLTARDEARGRAAVQQLQAEGLSPRFHQLDITD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SI A+ DFLR EYGGLDVLVNNAAIA+QL +PTP I A +TMKTNF GT ++CT LL
Sbjct: 67 LQSIHALRDFLRKEYGGLDVLVNNAAIAFQLSDPTPTPIKAEMTMKTNFFGTRDICTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PL+KPQG VV +SS +AL C PELQQ ET+TE+ELVGL+ F++D+K RK
Sbjct: 127 PLMKPQGRVVNMSSGWGFKALESCSPELQQKLRSETITEEELVGLMNKFVEDTKNGVHRK 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|NP_001029685.1| carbonyl reductase 1 [Bos taurus]
Length = 277
Score = 214 bits (545), Expect = 1e-55
Identities = 111/181 (61%), Positives = 137/181 (75%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKGIGF ++ DLC++F+GD++LT +D A G + QLQA+GL+P F LDI +
Sbjct: 7 VALVTGANKGIGFVIVRDLCRRFSGDVVLTARDEARGRAAVQQLQAEGLSPLFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
SIRA+ DFLR EYGGLDVLVNNA IA++ + TP HI A +TMKTNF GT +VCT LL
Sbjct: 67 RQSIRALRDFLRKEYGGLDVLVNNAGIAFKTADTTPFHIQAEVTMKTNFFGTRDVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PLIKPQG VV VSSF SV +L C ELQQ F ET+TE+ELVGL+ F++D+K RK
Sbjct: 127 PLIKPQGRVVNVSSFVSVNSLKKCSRELQQKFRSETITEEELVGLMNKFVEDTKNGVHRK 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|XP_869745.1| PREDICTED: similar to carbonyl reductase 3 isoform 2 [Bos taurus]
Length = 277
Score = 205 bits (522), Expect = 6e-53
Identities = 103/181 (56%), Positives = 135/181 (74%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKGIGFA+ DLC++F GD++LT +D A G + QLQA+GL+P F LDI +
Sbjct: 7 VALVTGANKGIGFAIARDLCREFPGDVVLTARDKARGRAAVQQLQAEGLSPRFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SIRA+ DFLR EYGGL+VLVNNA IA++ D+PTP I A +T+KTNF T NVCT LL
Sbjct: 67 LQSIRALRDFLRKEYGGLNVLVNNAGIAFKTDDPTPFDIQAEMTLKTNFFATRNVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
P++KP G VV VSS + +AL +C +LQ+ F ETLTE++LV L+K F++D+K +
Sbjct: 127 PIVKPHGRVVNVSSSQGSQALENCSEDLQEKFRCETLTEEDLVDLMKKFVEDTKNEVHER 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|XP_874747.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) (Prostaglandin-E(2) 9-reductase)
(Prostaglandin 9-ketoreductase) (15-hydroxyprostaglandin
dehydrogenase [NADP+]) [Bos taurus]
Length = 277
Score = 205 bits (522), Expect = 6e-53
Identities = 110/181 (60%), Positives = 132/181 (72%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKG+GFA++ L F GD++LT D A G + QLQAQGL+P F LDI +
Sbjct: 7 VALVTGANKGLGFAIVRALAGGFQGDVVLTAPDEAQGRAAVQQLQAQGLSPLFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
SIRA+ DFLR EYGGLDVLVNNA IA++ + TP HI A +TMKTNF GT +VCT LL
Sbjct: 67 RQSIRALRDFLRKEYGGLDVLVNNAGIAFKTADTTPFHIQAEVTMKTNFFGTRDVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PLIKPQG VV VSS SV +L C PELQQ F ET+TE+ELVGL+ F++D+K RK
Sbjct: 127 PLIKPQGRVVNVSSSVSVSSLKKCSPELQQKFQSETITEEELVGLMNKFVEDTKNGVHRK 186
Query: 667 D 669
+
Sbjct: 187 E 187
Score = 48.9 bits (115), Expect = 9e-06
Identities = 30/90 (33%), Positives = 44/90 (48%)
Frame = +3
Query: 615 GALEKFFARLKNKVHKKR*WIDSTLLMTKIGVPVIVKDLAQEPQISNNMEKLST*IPYCP 794
G + KF KN VH+K W D+ +TKIGV V+ + A++ +K+ CP
Sbjct: 170 GLMNKFVEDTKNGVHRKEGWPDTAYGVTKIGVTVLSRIHARKMSEQRGGDKILL-NACCP 228
Query: 795 WWIDNQHWGNPMPPVSQNNKRKTPLFLVLL 884
W+ G P P S +TP++L LL
Sbjct: 229 GWV-RTDMGGPKAPKSPEEGAETPVYLALL 257
>ref|XP_868956.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) (Prostaglandin-E(2) 9-reductase)
(Prostaglandin 9-ketoreductase) (15-hydroxyprostaglandin
dehydrogenase [NADP+]) [Bos taurus]
Length = 327
Score = 100 bits (248), Expect = 4e-21
Identities = 74/183 (40%), Positives = 95/183 (51%), Gaps = 2/183 (1%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
+ALVTGANKGIG A++L L GD +G +H+
Sbjct: 1 MALVTGANKGIGLAVLL-LGGAGTGD--------------------RGC----IHIRAAP 35
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLD--NPTPDHILANLTMKTNFLGTPNVCT* 480
S R +E+ L + + + TP I A +TMKTNF GT VCT
Sbjct: 36 RGSARRPPPLSFHEWRPLGGSPAAGISTHWISAADTTPFPIQAEVTMKTNFFGTRVVCTE 95
Query: 481 LLPLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCT 660
LLPL+KPQG VV VSS SV +L C PELQQ F ET+TE+ELVGL+ F++D+K
Sbjct: 96 LLPLLKPQGRVVNVSSSVSVTSLKKCSPELQQKFQSETITEEELVGLMNKFVEDTKNGVH 155
Query: 661 RKD 669
RK+
Sbjct: 156 RKE 158
>ref|XP_878838.1| PREDICTED: similar to carbonyl reductase 3 isoform 3 [Bos taurus]
Length = 169
Score = 82.8 bits (203), Expect = 6e-16
Identities = 39/79 (49%), Positives = 56/79 (70%)
Frame = +1
Query: 433 LTMKTNFLGTPNVCT*LLPLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDEL 612
+T+KTNF T NVCT LLP++KP G VV VSS + +AL +C +LQ+ F ETLTE++L
Sbjct: 1 MTLKTNFFATRNVCTELLPIVKPHGRVVNVSSSQGSQALENCSEDLQEKFRCETLTEEDL 60
Query: 613 VGLLKNFLQDSKTRCTRKD 669
V L+K F++D+K ++
Sbjct: 61 VDLMKKFVEDTKNEVHERE 79
>ref|XP_594165.2| PREDICTED: similar to carbonyl reductase 3 isoform 1 [Bos taurus]
Length = 169
Score = 82.8 bits (203), Expect = 6e-16
Identities = 39/79 (49%), Positives = 56/79 (70%)
Frame = +1
Query: 433 LTMKTNFLGTPNVCT*LLPLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDEL 612
+T+KTNF T NVCT LLP++KP G VV VSS + +AL +C +LQ+ F ETLTE++L
Sbjct: 1 MTLKTNFFATRNVCTELLPIVKPHGRVVNVSSSQGSQALENCSEDLQEKFRCETLTEEDL 60
Query: 613 VGLLKNFLQDSKTRCTRKD 669
V L+K F++D+K ++
Sbjct: 61 VDLMKKFVEDTKNEVHERE 79
Database: RefSeqBP
Posted date: Aug 1, 2006 7:14 PM
Number of letters in database: 16,112,626
Number of sequences in database: 33,508
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,678,884
Number of Sequences: 33508
Number of extensions: 1342331
Number of successful extensions: 3477
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 3060
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3451
length of database: 16,112,626
effective HSP length: 105
effective length of database: 12,594,286
effective search space used: 4483565816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqCP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-057194
(1386 letters)
Database: RefSeqCP
33,732 sequences; 19,266,565 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_852675.1| PREDICTED: similar to Carbonyl reductase [NADPH... 220 2e-57
Alignment gi|XP_537903.1| PREDICTED: similar to Carbonyl reductase [NADPH... 203 3e-52
Alignment gi|XP_544873.2| PREDICTED: similar to carbonyl reductase 3 [Can... 201 2e-51
Alignment gi|XP_535589.2| PREDICTED: similar to Carbonyl reductase [NADPH... 184 1e-46
Alignment gi|XP_533000.2| PREDICTED: similar to Retinol dehydrogenase 12 ... 53 8e-07
>ref|XP_852675.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) (Prostaglandin-E(2) 9-reductase)
(Prostaglandin 9-ketoreductase) (15-hydroxyprostaglandin
dehydrogenase [NADP+]) [Canis familiaris]
Length = 277
Score = 220 bits (561), Expect = 2e-57
Identities = 113/181 (62%), Positives = 139/181 (76%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKGIGFA+ +LC+QF+GD++LT +D A G + QLQA+GL+P F LDI +
Sbjct: 7 VALVTGANKGIGFAIARELCRQFSGDVVLTARDEARGRAAVQQLQAEGLSPRFHLLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SIRA+ DFLR EYGGLDVLVNNA IA++ ++PTP HI A +TMKTNF GT +VCT LL
Sbjct: 67 LQSIRALRDFLRKEYGGLDVLVNNAGIAFKTNDPTPFHIQAEVTMKTNFFGTRDVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PL+KPQG VV VSS SVRAL C PELQQ F E +TE+ELVGL+ F++D+K R
Sbjct: 127 PLMKPQGRVVNVSSVVSVRALKSCSPELQQKFRSEAITEEELVGLMNKFVEDTKKGVHRN 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|XP_537903.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) (Prostaglandin-E(2) 9-reductase)
(Prostaglandin 9-ketoreductase) (15-hydroxyprostaglandin
dehydrogenase [NADP+]) [Canis familiaris]
Length = 296
Score = 203 bits (517), Expect = 3e-52
Identities = 105/181 (58%), Positives = 131/181 (72%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VA+VTGANKG+GFA+ DLC+ F GD++LT++D A G + L+A+GL+P F LDI N
Sbjct: 7 VAVVTGANKGLGFAITRDLCRNFPGDVILTSRDEARGRAAVQHLKAEGLSPRFHLLDIDN 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SIRA+ DFL EYGGLDVLVNNA I + D+PTP HI A +T+KTNF GT ++CT LL
Sbjct: 67 LQSIRALRDFLWEEYGGLDVLVNNAGIVFTPDDPTPLHIQAEVTLKTNFFGTRDICTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PL+KPQG VV VSS S AL C PELQQ F ET+TE+EL L+ FL+D K +K
Sbjct: 127 PLVKPQGRVVNVSSIMSFLALKQCSPELQQKFTSETITEEELGMLMNKFLEDVKNGVHKK 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|XP_544873.2| PREDICTED: similar to carbonyl reductase 3 [Canis familiaris]
Length = 484
Score = 201 bits (510), Expect = 2e-51
Identities = 101/181 (55%), Positives = 134/181 (74%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGAN+GIGFA+ +LC+QF+GD++LT +D A G + QLQA+GL+P F LDI +
Sbjct: 214 VALVTGANRGIGFAIARELCRQFSGDVVLTARDEARGRAAVQQLQAEGLSPRFHLLDIDD 273
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SIRA+ DFLR EYGGL+VLVNNA IA++ D+PTP I A +T+KTNF T NVC LL
Sbjct: 274 LQSIRALRDFLRKEYGGLNVLVNNAGIAFKPDDPTPFDIQAEITLKTNFFATRNVCNELL 333
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
P+IKP G VV +SS E +AL +C +LQ+ F ETLTE++LV L+K F++D+ +
Sbjct: 334 PIIKPHGRVVNISSLEGSKALENCSADLQKKFQCETLTEEDLVDLMKKFVEDTSNEVHER 393
Query: 667 D 669
+
Sbjct: 394 E 394
>ref|XP_535589.2| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) (Prostaglandin-E(2) 9-reductase)
(Prostaglandin 9-ketoreductase) (15-hydroxyprostaglandin
dehydrogenase [NADP+]) [Canis familiaris]
Length = 292
Score = 184 bits (468), Expect = 1e-46
Identities = 98/181 (54%), Positives = 124/181 (68%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VA+VTGANKG+GFA+M DLC+ F+GD++LT +D A G + QLQ++GL+P F LDI +
Sbjct: 7 VAVVTGANKGLGFAIMRDLCRNFSGDVVLTARDEARGRAAVQQLQSKGLSPRFHLLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SI + DFL EY GLDVLVNNA I + +PTP I A +T+KTNF GT NVC LL
Sbjct: 67 LQSICTLRDFLCKEYRGLDVLVNNAGINFDTGDPTPLPIQAEVTLKTNFFGTRNVCRELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PL+KPQG VV VSS L C PELQQ F E +TE+EL L+ F++D K +K
Sbjct: 127 PLMKPQGRVVNVSSVMGFVTLKQCSPELQQKFTSEAITEEELGMLMNKFVEDVKNGVHKK 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|XP_533000.2| PREDICTED: similar to Retinol dehydrogenase 12 [Canis familiaris]
Length = 596
Score = 52.8 bits (125), Expect = 8e-07
Identities = 46/146 (31%), Positives = 75/146 (51%), Gaps = 4/146 (2%)
Frame = +1
Query: 130 ALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFL--HLDII 303
A+VTGAN GIG A+ +L ++ A ++L ++ G ++Q L +D+
Sbjct: 318 AVVTGANSGIGKAVCQELARRGAR-VILACRNWERGQKALAEIQVASKGTCLLLGQVDLS 376
Query: 304 NLNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*L 483
++ SIR+ +L EY + +LVNNAAI+ TP+ + +LT TN++G + L
Sbjct: 377 SMASIRSFARWLLQEYPEIHLLVNNAAISGFPKTLTPEGL--DLTFATNYVGPFLLTNLL 434
Query: 484 LPLIKPQGT--VVKVSSFESVRALHD 555
++ G+ VV VSSF D
Sbjct: 435 QGALQRAGSARVVNVSSFRHAHGYVD 460
Database: RefSeqCP
Posted date: Aug 1, 2006 9:25 PM
Number of letters in database: 19,266,565
Number of sequences in database: 33,732
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,307,248
Number of Sequences: 33732
Number of extensions: 1580713
Number of successful extensions: 3986
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 3454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3954
length of database: 19,266,565
effective HSP length: 106
effective length of database: 15,690,973
effective search space used: 5570295415
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqHP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-057194
(1386 letters)
Database: RefSeqHP
39,411 sequences; 17,774,539 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001748.1| carbonyl reductase 1 [Homo sapiens] 221 9e-58
Alignment gi|NP_001227.1| carbonyl reductase 3 [Homo sapiens] 201 9e-52
>ref|NP_001748.1| carbonyl reductase 1 [Homo sapiens]
Length = 277
Score = 221 bits (564), Expect = 9e-58
Identities = 113/181 (62%), Positives = 139/181 (76%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTG NKGIG A++ DLC+ F+GD++LT +DV G + QLQA+GL+P F LDI +
Sbjct: 7 VALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SIRA+ DFLR EYGGLDVLVNNA IA+++ +PTP HI A +TMKTNF GT +VCT LL
Sbjct: 67 LQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PLIKPQG VV VSS SVRAL C PELQQ F ET+TE+ELVGL+ F++D+K +K
Sbjct: 127 PLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQK 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|NP_001227.1| carbonyl reductase 3 [Homo sapiens]
Length = 277
Score = 201 bits (512), Expect = 9e-52
Identities = 104/200 (52%), Positives = 140/200 (70%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGAN+GIG A+ +LC+QF+GD++LT +DVA G + QLQA+GL+P F LDI +
Sbjct: 7 VALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
L SIRA+ DFLR EYGGL+VLVNNAA+A++ D+P P I A +T+KTNF T N+C LL
Sbjct: 67 LQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
P++KP G VV +SS + +RA +C +LQ+ F ETLTE +LV L+K F++D+K +
Sbjct: 127 PIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHER 186
Query: 667 DDGLIQPS**QKSGSL*LSR 726
+ P K G LSR
Sbjct: 187 EGWPNSPYGVSKLGVTVLSR 206
Database: RefSeqHP
Posted date: Aug 2, 2006 12:57 AM
Number of letters in database: 17,774,539
Number of sequences in database: 39,411
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,714,154
Number of Sequences: 39411
Number of extensions: 1549141
Number of successful extensions: 4127
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 3616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4099
length of database: 17,774,539
effective HSP length: 105
effective length of database: 13,636,384
effective search space used: 4854552704
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
Search to RefSeqMP
BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20060611S-057194
(1386 letters)
Database: RefSeqMP
45,328 sequences; 21,768,885 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_487442.1| PREDICTED: similar to Carbonyl reductase [NADPH... 209 4e-54
Alignment gi|XP_989090.1| PREDICTED: similar to Carbonyl reductase [NADPH... 209 4e-54
Alignment gi|NP_031646.1| carbonyl reductase 1 [Mus musculus] 203 4e-52
Alignment gi|NP_766635.1| carbonyl reductase 3 [Mus musculus] 202 7e-52
Alignment gi|XP_359206.3| PREDICTED: similar to Carbonyl reductase [NADPH... 192 5e-49
>ref|XP_487442.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) [Mus musculus]
Length = 277
Score = 209 bits (533), Expect = 4e-54
Identities = 107/182 (58%), Positives = 135/182 (74%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTG NKGIGF ++ DLCQ+F+GD++LT +D A G + +LQA+GLNP F LDI +
Sbjct: 7 VALVTGGNKGIGFEIIRDLCQKFSGDVVLTARDEARGCAAVQKLQAEGLNPRFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
SI A+ DFL EY GLDVLVNNA IA+++D+PTP HI +TMKTNF GT +VC +L
Sbjct: 67 PQSIHALRDFLLKEYKGLDVLVNNAGIAFKVDDPTPFHIKGEVTMKTNFFGTRDVCKEML 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PLIKPQG VV VSS S+ AL DC PELQQ F ET+TE+ELVGL+ +++D+K K
Sbjct: 127 PLIKPQGRVVNVSSMVSLSALKDCSPELQQKFRSETITEEELVGLMNKYVEDAKKGVYVK 186
Query: 667 DD 672
+D
Sbjct: 187 ED 188
>ref|XP_989090.1| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) [Mus musculus]
Length = 277
Score = 209 bits (533), Expect = 4e-54
Identities = 107/182 (58%), Positives = 135/182 (74%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTG NKGIGF ++ DLCQ+F+GD++LT +D A G + +LQA+GLNP F LDI +
Sbjct: 7 VALVTGGNKGIGFEIIRDLCQKFSGDVVLTARDEARGCAAVQKLQAEGLNPRFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
SI A+ DFL EY GLDVLVNNA IA+++D+PTP HI +TMKTNF GT +VC +L
Sbjct: 67 PQSIHALRDFLLKEYKGLDVLVNNAGIAFKVDDPTPFHIKGEVTMKTNFFGTRDVCKEML 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
PLIKPQG VV VSS S+ AL DC PELQQ F ET+TE+ELVGL+ +++D+K K
Sbjct: 127 PLIKPQGRVVNVSSMVSLSALKDCSPELQQKFRSETITEEELVGLMNKYVEDAKKGVYVK 186
Query: 667 DD 672
+D
Sbjct: 187 ED 188
>ref|NP_031646.1| carbonyl reductase 1 [Mus musculus]
Length = 277
Score = 203 bits (516), Expect = 4e-52
Identities = 104/174 (59%), Positives = 132/174 (75%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKGIGFA+ DLC++F+GD++L +D G + +LQA+GL+P F LDI N
Sbjct: 7 VALVTGANKGIGFAITRDLCRKFSGDVVLAARDEERGQTAVQKLQAEGLSPRFHQLDIDN 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
SIRA+ DFL EYGGLDVLVN A IA+++++ TP HI A +TM+TNF GT +VC LL
Sbjct: 67 PQSIRALRDFLLKEYGGLDVLVNKAGIAFKVNDDTPFHIQAEVTMETNFFGTRDVCKELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSK 648
PLIKPQG VV VSS S+RAL +C ELQQ F ET+TE+ELVGL+ F++D+K
Sbjct: 127 PLIKPQGRVVNVSSMVSLRALKNCRLELQQKFRSETITEEELVGLMNKFVEDTK 180
>ref|NP_766635.1| carbonyl reductase 3 [Mus musculus]
Length = 277
Score = 202 bits (514), Expect = 7e-52
Identities = 100/181 (55%), Positives = 136/181 (75%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
VALVTGANKGIGFA+ DLC++F+GD++LT +D A G + QLQA+GL+P F LDI +
Sbjct: 7 VALVTGANKGIGFAITRDLCRKFSGDVVLTARDEARGRAAVQQLQAEGLSPRFHQLDIDD 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
SIRA+ DFLR EYGGL+VLVNNA IA+++D+PTP I A +T+KTNF T NVCT LL
Sbjct: 67 PQSIRALRDFLRKEYGGLNVLVNNAGIAFRMDDPTPFDIQAEVTLKTNFFATRNVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSKTRCTRK 666
P++KP G VV +SS + ++AL +C +LQ+ F +TLTE +LV L+K F++D+K +
Sbjct: 127 PIMKPHGRVVNISSLQGLKALENCREDLQEKFRCDTLTEVDLVDLMKKFVEDTKNEVHER 186
Query: 667 D 669
+
Sbjct: 187 E 187
>ref|XP_359206.3| PREDICTED: similar to Carbonyl reductase [NADPH] 1 (NADPH-dependent
carbonyl reductase 1) [Mus musculus]
Length = 277
Score = 192 bits (489), Expect = 5e-49
Identities = 103/174 (59%), Positives = 126/174 (72%)
Frame = +1
Query: 127 VALVTGANKGIGFALMLDLCQQFAGDLMLTTKDVALGHST*NQLQAQGLNPHFLHLDIIN 306
+ALVTGANKGIGFA+ DLCQQF+GD++LT +D A G + +LQA+GL P F LDI +
Sbjct: 7 IALVTGANKGIGFAITRDLCQQFSGDVVLTARDEARGLAAVQKLQAEGLIPRFHQLDIND 66
Query: 307 LNSIRAMYDFLRNEYGGLDVLVNNAAIAYQLDNPTPDHILANLTMKTNFLGTPNVCT*LL 486
SI A+ +FL EYGGLDVLVNNA IAY+ + T HIL MKTNF GT VCT LL
Sbjct: 67 PQSIHALRNFLLKEYGGLDVLVNNAGIAYKGTDLTHFHILREAAMKTNFFGTQAVCTELL 126
Query: 487 PLIKPQGTVVKVSSFESVRALHDCHPELQQIF*KETLTEDELVGLLKNFLQDSK 648
PLIK QG VV +SS S+ AL +C ELQQ F ET+TE+ELVGL+ F++D+K
Sbjct: 127 PLIKTQGRVVNISSLISLEALKNCSLELQQKFRSETITEEELVGLMNKFVEDTK 180
Database: RefSeqMP
Posted date: Aug 2, 2006 12:58 AM
Number of letters in database: 21,768,885
Number of sequences in database: 45,328
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,413,955
Number of Sequences: 45328
Number of extensions: 1790468
Number of successful extensions: 4466
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 3973
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4430
length of database: 21,768,885
effective HSP length: 107
effective length of database: 16,918,789
effective search space used: 5989251306
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)