Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000299
(1039 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001192115.1| hypothetical protein LOC524810 [Bos taurus]. 253 1e-67
Alignment gi|XP_002684044.1| PREDICTED: Ig heavy chain-like, partial [Bos... 104 1e-22
Alignment gi|XP_002696847.1| PREDICTED: IgM [Bos taurus]. 103 2e-22
Alignment gi|XP_002696848.1| PREDICTED: IgM [Bos taurus]. 103 3e-22
Alignment gi|XP_002683686.1| PREDICTED: hypothetical protein [Bos taurus]. 89 8e-18
Alignment gi|NP_001077269.1| immunoglobulin lambda-like polypeptide 1 [Bo... 77 3e-14
Alignment gi|XP_002694682.1| PREDICTED: hCG96018-like [Bos taurus]. 60 4e-09
Alignment gi|XP_002691399.1| PREDICTED: IGK protein-like [Bos taurus]. 56 6e-08
Alignment gi|XP_002700829.1| PREDICTED: IGK protein-like [Bos taurus]. 56 6e-08
Alignment gi|XP_002701782.1| PREDICTED: immunoglobulin lambda-like polype... 52 7e-07
>ref|NP_001192115.1| hypothetical protein LOC524810 [Bos taurus].
Length = 487
Score = 253 bits (647), Expect = 1e-67
Identities = 142/306 (46%), Positives = 184/306 (60%), Gaps = 16/306 (5%)
Frame = +1
Query: 85 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGL 258
W +LF L +GV + +L ESG LV+P +L L+C SGF+ SSY + WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTASGFSLSSYGINWVRQAPGKAL 64
Query: 259 EWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGG----- 423
E L I+ G+ T Y ++K R I++D S++ L ++S+ TEDTA YYC
Sbjct: 65 ECLACITGGGN-TAYNPALKSRLNITKDKSKSQVSLSLSSVTTEDTATYYCAKYSTDDCA 123
Query: 424 --------HLWGPGXXXXXXXXXXTSPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTV 579
WG G TSP IFPL+LG+++PAG VVI CLV+ FFPS PL+V
Sbjct: 124 CSSERNYIDAWGQGLLVTVSSESETSPSIFPLSLGNNDPAGQVVIGCLVQGFFPSAPLSV 183
Query: 580 TWSPSREGVIVRNFPPAQAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPC 759
TW+ + + V VRNFP AG LYTMSSQLTLP CP Q + CQVQHLSK+S++V VPC
Sbjct: 184 TWNQNGDSVSVRNFPAVLAGSLYTMSSQLTLPASLCPKGQSVTCQVQHLSKASKTVAVPC 243
Query: 760 KVLPSDP-CPQCCKPSLSLQPPALADLLLGSMPASTAHSVA*KNPRVSASPGSPQVGKMP 936
+ S C C+PSLS+QPPAL DLLLGS + T K+ ++ +P GK
Sbjct: 244 IIQDSSSCCVPNCEPSLSVQPPALEDLLLGSNASLTCTLSGLKSAEGASFTWNPTGGKTA 303
Query: 937 SQRPPR 954
Q P+
Sbjct: 304 VQGSPK 309
Score = 112 bits (281), Expect = 4e-25
Identities = 49/65 (75%), Positives = 54/65 (83%)
Frame = +3
Query: 843 GLHASLNCTLSGLKKSEGVSFTWQPSGGKDAVPASPTRDSCGCYSVSSILPGCADPWNKE 1022
G +ASL CTLSGLK +EG SFTW P+GGK AV SP RDSCGCYSVSS+LPGCADPWN
Sbjct: 273 GSNASLTCTLSGLKSAEGASFTWNPTGGKTAVQGSPKRDSCGCYSVSSVLPGCADPWNSG 332
Query: 1023 KTFSC 1037
+TFSC
Sbjct: 333 QTFSC 337
>ref|XP_002684044.1| PREDICTED: Ig heavy chain-like, partial [Bos taurus].
Length = 121
Score = 104 bits (259), Expect = 1e-22
Identities = 55/111 (49%), Positives = 76/111 (68%), Gaps = 2/111 (1%)
Frame = +1
Query: 85 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGL 258
W +LF L +GV + +L ESG LV+P +L L+C SGF+ SSY++ WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTISGFSLSSYAVGWVRQAPGKAL 64
Query: 259 EWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
EW+ ISS G ST ++K R +I++DNS++ L ++S+ TEDTA YYC
Sbjct: 65 EWVGGISSGG-STCLNPALKSRLSITKDNSKSQVSLSVSSVTTEDTATYYC 114
>ref|XP_002696847.1| PREDICTED: IgM [Bos taurus].
Length = 193
Score = 103 bits (257), Expect = 2e-22
Identities = 55/111 (49%), Positives = 74/111 (66%), Gaps = 2/111 (1%)
Frame = +1
Query: 85 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGL 258
W +LF L +GV + +L ESG LV+P +L L+C SGF+ S S+ WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTVSGFSLSDNSVGWVRQAPGKAL 64
Query: 259 EWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
EWL I S G ST Y ++K R +I++DNS++ L ++S+ TEDTA YYC
Sbjct: 65 EWLGVIYSGG-STGYNPALKSRLSITKDNSKSQVSLSLSSVTTEDTATYYC 114
>ref|XP_002696848.1| PREDICTED: IgM [Bos taurus].
Length = 259
Score = 103 bits (256), Expect = 3e-22
Identities = 53/111 (47%), Positives = 74/111 (66%), Gaps = 2/111 (1%)
Frame = +1
Query: 85 WVVLFALL--QGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGL 258
W +LF L +GV + +L ESG LV+P +L L+C SGF+ SS + WVRQAPGK L
Sbjct: 5 WTLLFVLSAPRGVLSQVQLRESGPSLVKPSQTLSLTCTVSGFSLSSNGVGWVRQAPGKAL 64
Query: 259 EWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
EW+ I + GD TYY ++K R +I++DNS++ L ++S+ EDTA YYC
Sbjct: 65 EWVGGIDNDGD-TYYNPALKSRLSITKDNSKSQVSLSVSSVTPEDTATYYC 114
>ref|XP_002683686.1| PREDICTED: hypothetical protein [Bos taurus].
Length = 226
Score = 88.6 bits (218), Expect = 8e-18
Identities = 49/112 (43%), Positives = 71/112 (63%), Gaps = 4/112 (3%)
Frame = +1
Query: 85 WVVLFALLQGVQG---EEKLVESGGGLVQPGGSLRLSCVGSGFTFSS-YSMRWVRQAPGK 252
W +L L+ G QG + +L ESG L++ +L L+C GSG++ +S Y W+RQAPGK
Sbjct: 4 WGLLLCLVTGPQGVLSQVQLQESGPELIKSSQTLSLTCAGSGYSITSDYGRNWIRQAPGK 63
Query: 253 GLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYY 408
GLE + + +GD TYY+ S+K +I RD +N LQ++S+ TEDTA Y
Sbjct: 64 GLEQMGCMYYNGD-TYYSPSIKSHTSICRDTPKNQFSLQLSSVPTEDTAMCY 114
>ref|NP_001077269.1| immunoglobulin lambda-like polypeptide 1 [Bos taurus].
Length = 235
Score = 77.0 bits (188), Expect = 3e-14
Identities = 61/222 (27%), Positives = 92/222 (41%), Gaps = 10/222 (4%)
Frame = +1
Query: 88 VVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWL 267
+ L AL G + L + G + ++C GS Y + W +Q PG GL+ +
Sbjct: 8 LTLVALCTGSWAQAVLTQPSSVSGSLGQRVSITCSGSSTNIGIYGVNWYQQVPGSGLKTI 67
Query: 268 TYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGGH-----LW 432
Y + Y V RF+ S+ S NTA L +NSL+ ED A Y+C G + ++
Sbjct: 68 IY-----EDKYRPSGVPDRFSGSK--SGNTATLTINSLQAEDEADYFCAAGDYSVNTAVF 120
Query: 433 GPGXXXXXXXXXXTSPKIFPLTLGSSEPAG-YVVIACLVRDFFPSEPLTVTWSPSREGVI 609
G G + P + + E G + CL+ DF+P +TV W + I
Sbjct: 121 GGGTTLTVLGQPKSPPSVTLFPPSTEELNGNKATLVCLISDFYPGS-VTVVWK-ADGSTI 178
Query: 610 VRNFPPA----QAGGLYTMSSQLTLPVEQCPADQILKCQVQH 723
RN Q+ Y SS L+L + C+V H
Sbjct: 179 TRNVETTRASKQSNSKYAASSYLSLTSSDWKSKGSYSCEVTH 220
>ref|XP_002694682.1| PREDICTED: hCG96018-like [Bos taurus].
Length = 150
Score = 59.7 bits (143), Expect = 4e-09
Identities = 33/83 (39%), Positives = 45/83 (54%), Gaps = 1/83 (1%)
Frame = +1
Query: 166 GGSLRLSC-VGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRD 342
G S RLSC + SG+ S+YS+ W +Q G L +L S D + V RF+ S+D
Sbjct: 34 GASARLSCTLSSGYNVSNYSIYWYQQKAGNPLRYLLRFKSDSDK-HQGSGVPSRFSGSKD 92
Query: 343 NSQNTAYLQMNSLRTEDTARYYC 411
S N L ++ L+ ED A YYC
Sbjct: 93 ASTNAGLLLISGLQPEDEADYYC 115
>ref|XP_002691399.1| PREDICTED: IGK protein-like [Bos taurus].
Length = 235
Score = 55.8 bits (133), Expect = 6e-08
Identities = 57/228 (25%), Positives = 91/228 (39%), Gaps = 9/228 (3%)
Frame = +1
Query: 91 VLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLT 270
+L L G +G+ ++ +S L G R+S + W +Q PGK + L
Sbjct: 10 LLLLWLPGKEGDIQVTQSPSSLSASLGD-RVSITCQASQSIDTKLAWYQQKPGKAPKLLI 68
Query: 271 YISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGG---HLWGPG 441
Y S + +F+ S + T L ++SL+ +D A YYCL + +G G
Sbjct: 69 YAIPRSPSWF-----PSQFSGSGFGADFT--LTISSLKADDIATYYCLSRNCCVNTFGQG 121
Query: 442 XXXXXXXXXXTSPKIFPLTLGSSE-PAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRN 618
P +F + G V + CLV DF+P + + V W N
Sbjct: 122 TKVEIKRSD-AEPSVFLFKPSDEQLKTGTVSVVCLVNDFYPKD-INVKWKVDGVTQSSSN 179
Query: 619 FPPA-----QAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKSSQSV 747
F + Y++SS LTLP + + C+V H S ++ V
Sbjct: 180 FQNSFTDQDSKKSTYSLSSILTLPSSEYQSHDAYTCEVSHKSLTTTLV 227
>ref|XP_002700829.1| PREDICTED: IGK protein-like [Bos taurus].
Length = 235
Score = 55.8 bits (133), Expect = 6e-08
Identities = 57/228 (25%), Positives = 91/228 (39%), Gaps = 9/228 (3%)
Frame = +1
Query: 91 VLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLT 270
+L L G +G+ ++ +S L G R+S + W +Q PGK + L
Sbjct: 10 LLLLWLPGKEGDIQVTQSPSSLSASLGD-RVSITCQASQSIDTKLAWYQQKPGKAPKLLI 68
Query: 271 YISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGG---HLWGPG 441
Y S + +F+ S + T L ++SL+ +D A YYCL + +G G
Sbjct: 69 YAIPRSPSWF-----PSQFSGSGFGADFT--LTISSLKADDIATYYCLSRNCCVNTFGQG 121
Query: 442 XXXXXXXXXXTSPKIFPLTLGSSE-PAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRN 618
P +F + G V + CLV DF+P + + V W N
Sbjct: 122 TKVEIKRSD-AEPSVFLFKPSDEQLKTGTVSVVCLVNDFYPKD-INVKWKVDGVTQSSSN 179
Query: 619 FPPA-----QAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKSSQSV 747
F + Y++SS LTLP + + C+V H S ++ V
Sbjct: 180 FQNSFTDQDSKKSTYSLSSILTLPSSEYQSHDAYTCEVSHKSLTTTLV 227
>ref|XP_002701782.1| PREDICTED: immunoglobulin lambda-like polypeptide 1-like [Bos
taurus].
Length = 236
Score = 52.4 bits (124), Expect = 7e-07
Identities = 54/223 (24%), Positives = 80/223 (35%), Gaps = 13/223 (5%)
Frame = +1
Query: 94 LFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTY 273
L L G +L + V G + ++C G S W +Q PG+ + Y
Sbjct: 10 LLTLCTGHLASSQLTQPPAVSVSLGQTASITCQGDDLEL--LSAHWYQQKPGQAPVLVIY 67
Query: 274 ISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGG--------HL 429
+ A + RF+ S+ S TA L + + ED A YYC L
Sbjct: 68 ADDN-----LASGIPDRFSGSK--SDTTATLTIRGAQAEDEADYYCQSADISGPVSLCDL 120
Query: 430 WGPGXXXXXXXXXXTSPKIFPLTLGSSE-PAGYVVIACLVRDFFPSEPLTVTWSPSREGV 606
+G G + P + E A + CL+ DF+P +TV W +
Sbjct: 121 FGGGTRVTVLGQPKSPPSVTLFPPSKEELSANKATLVCLISDFYPGS-VTVAWK-ADGST 178
Query: 607 IVRNFPPA----QAGGLYTMSSQLTLPVEQCPADQILKCQVQH 723
I RN Q+ Y SS L+L + C+V H
Sbjct: 179 ITRNVKTTRASKQSNSKYAASSYLSLTDSDWKSKGSYSCEVTH 221
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 49,714,434
Number of extensions: 1668114
Number of successful extensions: 8468
Number of sequences better than 1.0e-05: 13
Number of HSP's gapped: 8341
Number of HSP's successfully gapped: 14
Length of query: 346
Length of database: 17,681,374
Length adjustment: 103
Effective length of query: 243
Effective length of database: 14,273,310
Effective search space: 3468414330
Effective search space used: 3468414330
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000299
(1039 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_848935.1| PREDICTED: similar to Ig heavy chain V-III regi... 192 4e-49
Alignment gi|XP_849253.1| PREDICTED: similar to Ig heavy chain V-III regi... 191 1e-48
Alignment gi|XP_848432.1| PREDICTED: similar to Ig heavy chain V-III regi... 189 4e-48
Alignment gi|XP_848877.1| PREDICTED: similar to Ig heavy chain V-III regi... 187 2e-47
Alignment gi|XP_848796.1| PREDICTED: similar to Ig heavy chain V-III regi... 186 2e-47
Alignment gi|XP_848501.1| PREDICTED: similar to Ig heavy chain V-III regi... 185 5e-47
Alignment gi|XP_848616.1| PREDICTED: similar to Ig heavy chain V-III regi... 185 5e-47
Alignment gi|XP_848599.1| PREDICTED: similar to Ig heavy chain V-III regi... 185 5e-47
Alignment gi|XP_849354.1| PREDICTED: similar to Ig heavy chain V-III regi... 182 6e-46
Alignment gi|XP_848321.1| PREDICTED: similar to Ig heavy chain V-III regi... 182 6e-46
>ref|XP_848935.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 315
Score = 192 bits (488), Expect = 4e-49
Identities = 94/126 (74%), Positives = 103/126 (81%)
Frame = +1
Query: 34 SVIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFS 213
SVIR +HR ME L WV L A+LQGVQGE +LVESGG L +PGGSLRLSCV SG TFS
Sbjct: 156 SVIRTQHRQITMESVLGWVFLVAILQGVQGEVQLVESGGDLAKPGGSLRLSCVASGLTFS 215
Query: 214 SYSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTED 393
SYSM WVRQAPGKGL+W+T IS G STYY D+VKGRFTISRDN++NT YLQMNSLR ED
Sbjct: 216 SYSMSWVRQAPGKGLQWVTAISYDGSSTYYTDAVKGRFTISRDNARNTVYLQMNSLRAED 275
Query: 394 TARYYC 411
TA YYC
Sbjct: 276 TAVYYC 281
>ref|XP_849253.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 142
Score = 191 bits (484), Expect = 1e-48
Identities = 93/126 (73%), Positives = 107/126 (84%)
Frame = +1
Query: 34 SVIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFS 213
S IR E+R ME + WV L A+L+GVQGE +LVESGG LV+PGGSLRLSCV SGFTFS
Sbjct: 3 SGIRVENRRLNMESFVGWVFLVAILKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFS 62
Query: 214 SYSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTED 393
SY+M WVRQAPGKGL+W+ YI+S G STYYAD+VKGRFTISRDN++NT YLQMNSLR+ED
Sbjct: 63 SYAMSWVRQAPGKGLQWVAYINSGGSSTYYADAVKGRFTISRDNARNTLYLQMNSLRSED 122
Query: 394 TARYYC 411
TA YYC
Sbjct: 123 TAVYYC 128
>ref|XP_848432.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 267
Score = 189 bits (480), Expect = 4e-48
Identities = 91/124 (73%), Positives = 102/124 (82%)
Frame = +1
Query: 49 EHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMR 228
EHR ME L+WV L +L+GVQGE +LVESGG LV+PGGSLRLSCV SGFTFSSY M
Sbjct: 104 EHRQRTMESALSWVFLVTILKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFSSYHMS 163
Query: 229 WVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYY 408
WVRQAPGKGL+W+ YI+S G ST YAD+VKGRFTISRDN++NT YLQMNSLR EDTA YY
Sbjct: 164 WVRQAPGKGLQWVAYINSGGSSTSYADAVKGRFTISRDNAKNTLYLQMNSLRAEDTAVYY 223
Query: 409 CLGG 420
C G
Sbjct: 224 CAKG 227
>ref|XP_848877.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 250
Score = 187 bits (474), Expect = 2e-47
Identities = 90/125 (72%), Positives = 102/125 (81%)
Frame = +1
Query: 37 VIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSS 216
+IR + R ME L+WV L A+LQGVQGE +LVESGG LV+PGGSLRLSCV SGFTFS+
Sbjct: 108 LIRTQQRQTTMESVLSWVFLVAILQGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFSN 167
Query: 217 YSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 396
Y M WVRQAPGKGL+W+ IS G STYY D+VKGRFTISRDN++NT YLQMNSLR EDT
Sbjct: 168 YDMSWVRQAPGKGLQWVAAISYDGSSTYYTDAVKGRFTISRDNARNTVYLQMNSLRAEDT 227
Query: 397 ARYYC 411
A YYC
Sbjct: 228 AVYYC 232
Score = 138 bits (348), Expect(2) = 6e-33
Identities = 66/89 (74%), Positives = 73/89 (82%)
Frame = +1
Query: 145 GGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGR 324
G LV+PGGSLRLSCV SGFTFS Y+M WV QAPGKGL+W+ ISSSG STYY D+VKGR
Sbjct: 10 GEDLVKPGGSLRLSCVTSGFTFSRYAMSWVGQAPGKGLQWVAAISSSGSSTYYVDAVKGR 69
Query: 325 FTISRDNSQNTAYLQMNSLRTEDTARYYC 411
FTIS DN++N YLQMNSLR ED A YYC
Sbjct: 70 FTISIDNAKNMVYLQMNSLRAEDIAVYYC 98
Score = 21.6 bits (44), Expect(2) = 6e-33
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = +2
Query: 122 VRRSWWSLEEAWCSLGG 172
V+ SWWSL E GG
Sbjct: 2 VKCSWWSLGEDLVKPGG 18
>ref|XP_848796.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 244
Score = 186 bits (473), Expect = 2e-47
Identities = 90/123 (73%), Positives = 101/123 (82%)
Frame = +1
Query: 43 RKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYS 222
+ EH+ ME L WV L A+L+GVQ E +LVESGG LV+P GSLRLSCV SGFTFSSY
Sbjct: 70 KTEHKQLTMESVLCWVFLVAILKGVQSEVQLVESGGDLVKPVGSLRLSCVASGFTFSSYD 129
Query: 223 MRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTAR 402
M WVRQAPGKGL+W+ YISS G STYYAD+VKGRFTISRDN++NT YLQMNSLR EDTA
Sbjct: 130 MNWVRQAPGKGLQWVAYISSGGSSTYYADAVKGRFTISRDNAKNTLYLQMNSLRAEDTAM 189
Query: 403 YYC 411
YYC
Sbjct: 190 YYC 192
>ref|XP_848501.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 162
Score = 185 bits (470), Expect = 5e-47
Identities = 90/124 (72%), Positives = 103/124 (83%)
Frame = +1
Query: 40 IRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSY 219
IR E+R M+ L WV L A+L+GVQGE +LVESGG LV+PGGSLRLSCV SGFTFSSY
Sbjct: 4 IRTEYRQLTMKLVLGWVFLVAILKGVQGEVQLVESGGDLVKPGGSLRLSCVASGFTFSSY 63
Query: 220 SMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA 399
+M WVRQAPGKGL+W+ +I SG ST YAD+VKGRFTISRDN++NT YLQMNSLR EDTA
Sbjct: 64 NMGWVRQAPGKGLQWVAWIYDSGSSTSYADAVKGRFTISRDNAKNTLYLQMNSLRAEDTA 123
Query: 400 RYYC 411
YYC
Sbjct: 124 VYYC 127
>ref|XP_848616.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 186
Score = 185 bits (470), Expect = 5e-47
Identities = 92/129 (71%), Positives = 103/129 (79%)
Frame = +1
Query: 37 VIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSS 216
VIR +HR ME L+WV L A+LQGVQGE +LVESGG LV+P GSLRLSCV SGFTFSS
Sbjct: 22 VIRTQHRQITMESVLSWVFLVAILQGVQGEVQLVESGGDLVKPAGSLRLSCVASGFTFSS 81
Query: 217 YSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 396
YSM WVRQAP KGL+ + I+S G STYY D+VKGRFTISRDN++NT YLQMNSLR EDT
Sbjct: 82 YSMSWVRQAPEKGLQLVAGINSGGSSTYYTDAVKGRFTISRDNAKNTVYLQMNSLRAEDT 141
Query: 397 ARYYCLGGG 423
A YYC G
Sbjct: 142 AMYYCAEAG 150
>ref|XP_848599.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 201
Score = 185 bits (470), Expect = 5e-47
Identities = 93/133 (69%), Positives = 104/133 (78%)
Frame = +1
Query: 40 IRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSY 219
IR+E R ME L+WV L +L+GVQ E +LVESGG LV+P GSLRLSCV SGFTFSSY
Sbjct: 40 IRRELRKLTMESVLSWVFLVTILKGVQCEVQLVESGGDLVKPEGSLRLSCVVSGFTFSSY 99
Query: 220 SMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA 399
M WVRQAPGKGL+W+ YISS G ST Y D+VKGRFTISRDN++NT YLQMNSLRTEDTA
Sbjct: 100 DMSWVRQAPGKGLQWVAYISSDGRSTSYTDAVKGRFTISRDNAKNTLYLQMNSLRTEDTA 159
Query: 400 RYYCLGGGHLWGP 438
YYC G W P
Sbjct: 160 VYYCAAGSLGWFP 172
>ref|XP_849354.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 350
Score = 182 bits (461), Expect = 6e-46
Identities = 88/125 (70%), Positives = 103/125 (82%)
Frame = +1
Query: 37 VIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSS 216
+IR + + +ME L WV L A+L+GVQGE +LVE GG LV+PGGSLRLSCV SGFTFSS
Sbjct: 132 LIRTQRKQTSMESVLGWVFLVAILKGVQGELQLVELGGDLVKPGGSLRLSCVASGFTFSS 191
Query: 217 YSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 396
Y+M WV QAPGKGL+W+ ISSSG STY+ D+VKGRFTISRDN++NT YLQMNSLR EDT
Sbjct: 192 YAMSWVCQAPGKGLQWVAAISSSGSSTYHVDAVKGRFTISRDNAKNTVYLQMNSLRAEDT 251
Query: 397 ARYYC 411
A YYC
Sbjct: 252 AVYYC 256
Score = 161 bits (407), Expect = 1e-39
Identities = 79/115 (68%), Positives = 92/115 (80%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
ME L+ V L A+L+GVQGE LVESGG LV+P GSLR SCV SGFTFSSY + WVRQAP
Sbjct: 1 MESALHRVSLVAILKGVQGEVPLVESGGELVKPEGSLRFSCVASGFTFSSYWISWVRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
GKGL W++ I+ G +TY+AD+VKGRFTISRDN++NT YLQMNSLR E T YYC
Sbjct: 61 GKGLHWVSVINKDGSTTYHADAVKGRFTISRDNAKNTLYLQMNSLRAEGTTVYYC 115
Score = 110 bits (275), Expect = 2e-24
Identities = 53/72 (73%), Positives = 59/72 (81%)
Frame = +1
Query: 115 VQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGDS 294
VQGE +LVESGG LV+P G LRLSCV SGFTFSSY M WVRQAPGK L+W+ YI+S G S
Sbjct: 266 VQGEVQLVESGGDLVKPCGLLRLSCVASGFTFSSYIMSWVRQAPGKWLQWVAYINSGGSS 325
Query: 295 TYYADSVKGRFT 330
T Y D+VKGRFT
Sbjct: 326 TRYTDAVKGRFT 337
>ref|XP_848321.1| PREDICTED: similar to Ig heavy chain V-III region VH26 precursor
[Canis familiaris].
Length = 296
Score = 182 bits (461), Expect = 6e-46
Identities = 88/124 (70%), Positives = 102/124 (82%)
Frame = +1
Query: 40 IRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSY 219
IR E+R ME L+WV L A L+GVQGE +LVESGG +V+PGGSLRLSCV SGFTFSSY
Sbjct: 138 IRTEYRQLTMESVLSWVFLVATLKGVQGEVQLVESGGDMVKPGGSLRLSCVASGFTFSSY 197
Query: 220 SMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA 399
M W RQAPGKGL+W+++I+ G ST YAD+VKGRFTISRDN++NT YLQMNSLR EDTA
Sbjct: 198 YMYWARQAPGKGLQWVSHINKDGSSTSYADAVKGRFTISRDNAKNTLYLQMNSLRAEDTA 257
Query: 400 RYYC 411
YYC
Sbjct: 258 VYYC 261
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 51,740,647
Number of extensions: 1702840
Number of successful extensions: 8635
Number of sequences better than 1.0e-05: 123
Number of HSP's gapped: 8482
Number of HSP's successfully gapped: 134
Length of query: 346
Length of database: 18,874,504
Length adjustment: 104
Effective length of query: 242
Effective length of database: 15,407,560
Effective search space: 3728629520
Effective search space used: 3728629520
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000299
(1039 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_003403902.1| PREDICTED: ig heavy chain V-III region VH26-... 184 2e-46
Alignment gi|XP_003403714.1| PREDICTED: hypothetical protein LOC100653084... 181 1e-45
Alignment gi|XP_003403901.1| PREDICTED: ig heavy chain V-III region VH26-... 179 4e-45
Alignment gi|XP_003403513.1| PREDICTED: ig heavy chain V-III region VH26-... 179 5e-45
Alignment gi|XP_003403879.1| PREDICTED: ig heavy chain V-III region VH26-... 178 6e-45
Alignment gi|XP_001718089.4| PREDICTED: hypothetical protein LOC100132941... 177 2e-44
Alignment gi|XP_002346315.2| PREDICTED: ig heavy chain V-III region VH26-... 177 2e-44
Alignment gi|XP_003120167.2| PREDICTED: hypothetical protein LOC100291917... 171 8e-43
Alignment gi|XP_001718156.4| PREDICTED: ig heavy chain V-III region VH26-... 169 4e-42
Alignment gi|XP_003119639.1| PREDICTED: hypothetical protein LOC100508797... 167 2e-41
>ref|XP_003403902.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 231
Score = 184 bits (466), Expect = 2e-46
Identities = 90/116 (77%), Positives = 103/116 (88%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV+L A+L+ VQ E +LVESGGGLVQPGGSLRLSC SGFTFSSY+M WVRQAP
Sbjct: 1 MEFWLSWVLLVAILKDVQCEVQLVESGGGLVQPGGSLRLSCSASGFTFSSYAMHWVRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCL 414
GKGLE+++ ISS+G STYYADSVKGRFTISRDNS+NT YLQM+SLR EDTA YYC+
Sbjct: 61 GKGLEYVSAISSNGGSTYYADSVKGRFTISRDNSKNTLYLQMSSLRAEDTAVYYCV 116
>ref|XP_003403714.1| PREDICTED: hypothetical protein LOC100653084 [Homo sapiens].
Length = 279
Score = 181 bits (459), Expect = 1e-45
Identities = 88/120 (73%), Positives = 98/120 (81%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV L A+L+GVQ E +LVESGG +VQPGGSLRLSC SGFTF Y+M WVRQAP
Sbjct: 1 MEFGLSWVFLVAILKGVQCEVQLVESGGVVVQPGGSLRLSCAASGFTFDDYAMHWVRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGGH 426
GKGLEW++ IS G STYYADSVKGRFTISRDNS+N+ YLQMNSLR EDTA YYC H
Sbjct: 61 GKGLEWVSLISWDGGSTYYADSVKGRFTISRDNSKNSLYLQMNSLRAEDTALYYCAKDTH 120
Score = 95.5 bits (236), Expect = 7e-20
Identities = 51/92 (55%), Positives = 63/92 (68%), Gaps = 2/92 (2%)
Frame = +1
Query: 163 PGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGDS--TYYADSVKGRFTIS 336
PG S + SGFTFS + M W+RQA GKGLEW+ I + +S T A SVKGRFTIS
Sbjct: 123 PGESWQGEGYSSGFTFSDFWMHWIRQASGKGLEWVGRIRTKRNSYTTECAASVKGRFTIS 182
Query: 337 RDNSQNTAYLQMNSLRTEDTARYYCLGGGHLW 432
RD+S+NT Y+QMN+L+T+ TA YY G G W
Sbjct: 183 RDDSKNTLYMQMNTLKTKYTAIYYSPGQGLEW 214
Score = 53.9 bits (128), Expect = 2e-07
Identities = 25/55 (45%), Positives = 35/55 (63%)
Frame = +1
Query: 241 APGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARY 405
+PG+GLEW+ +I + + Y GRF S D S + AYLQ++SL+ EDTA Y
Sbjct: 207 SPGQGLEWMGWIITYTGNPMYTHGFTGRFVFSMDTSVSMAYLQISSLKAEDTAVY 261
>ref|XP_003403901.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 186
Score = 179 bits (454), Expect = 4e-45
Identities = 85/115 (73%), Positives = 98/115 (85%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV L A+++GVQ + +LVESGGGLV+PGGSLRLSC SGFTFS Y M W+RQAP
Sbjct: 1 MEFGLSWVFLVAIIKGVQCQVQLVESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
GKGLEW++YISSS T YADSVKGRFTISRDN++N+ YLQMNSLR EDTA YYC
Sbjct: 61 GKGLEWVSYISSSSSYTNYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYC 115
>ref|XP_003403513.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 147
Score = 179 bits (453), Expect = 5e-45
Identities = 87/115 (75%), Positives = 99/115 (86%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV L A+L+GVQ E +LV+SGGGLVQPGGSLRLSC GSGFTFSSY+M WVRQAP
Sbjct: 1 MEFVLSWVFLVAILKGVQCEVQLVQSGGGLVQPGGSLRLSCAGSGFTFSSYAMHWVRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
GKGLEW++ I + G TYYADSVKGRFTISRDN++N+ YLQMNSLR ED A YYC
Sbjct: 61 GKGLEWVSAIGTGG-GTYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDMAVYYC 114
>ref|XP_003403879.1| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 173
Score = 178 bits (452), Expect = 6e-45
Identities = 87/115 (75%), Positives = 95/115 (82%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV L ALL+GVQ + +LVESGGG+VQPG SLRLSC SGFTFSSY M WVRQAP
Sbjct: 1 MEFGLSWVFLVALLRGVQCQVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
GKGLEW+ IS G + YYADSVKGRFTISRDNS+NT YLQMNSLR DTA YYC
Sbjct: 61 GKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAGDTAVYYC 115
>ref|XP_001718089.4| PREDICTED: hypothetical protein LOC100132941 [Homo sapiens].
Length = 297
Score = 177 bits (448), Expect = 2e-44
Identities = 86/115 (74%), Positives = 98/115 (85%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV L A+L+GVQ E +LV+SGGGLV PGGSLRLSC GSGFTFSSY+M WVRQAP
Sbjct: 1 MEFVLSWVFLVAILKGVQCEVQLVQSGGGLVHPGGSLRLSCAGSGFTFSSYAMHWVRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
GKGLEW++ I + G TYYADSVKGRFTISRDN++N+ YLQMNSLR ED A YYC
Sbjct: 61 GKGLEWVSAIGTGG-GTYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDMAVYYC 114
>ref|XP_002346315.2| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 147
Score = 177 bits (448), Expect = 2e-44
Identities = 86/115 (74%), Positives = 98/115 (85%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV L A+L+GVQ E +LV+SGGGLV PGGSLRLSC GSGFTFSSY+M WVRQAP
Sbjct: 1 MEFVLSWVFLVAILKGVQCEVQLVQSGGGLVHPGGSLRLSCAGSGFTFSSYAMHWVRQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
GKGLEW++ I + G TYYADSVKGRFTISRDN++N+ YLQMNSLR ED A YYC
Sbjct: 61 GKGLEWVSAIGTGG-GTYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDMAVYYC 114
>ref|XP_003120167.2| PREDICTED: hypothetical protein LOC100291917 [Homo sapiens].
Length = 295
Score = 171 bits (434), Expect = 8e-43
Identities = 85/124 (68%), Positives = 99/124 (79%)
Frame = +1
Query: 40 IRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSY 219
+ K + E L+W+ L A+L+GVQ E +LVESGGGLV+PGGSLRLSC SGFTFS Y
Sbjct: 13 LHKSEKRTTKESWLSWLFLVAILKGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFSDY 72
Query: 220 SMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA 399
M WVRQAPGKGLEW++ ISSS + YYADSVKGRFTISRDN++N+ YLQMNSLR EDTA
Sbjct: 73 YMNWVRQAPGKGLEWVSSISSS-STIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTA 131
Query: 400 RYYC 411
YYC
Sbjct: 132 VYYC 135
Score = 86.7 bits (213), Expect = 3e-17
Identities = 47/101 (46%), Positives = 61/101 (60%), Gaps = 2/101 (1%)
Frame = +1
Query: 115 VQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMR--WVRQAPGKGLEWLTYISSSG 288
V + L ESG LV+P +L L+C SGF+ S+ MR W+RQ PGK LEWL I
Sbjct: 164 VLSQVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGMRVSWIRQPPGKALEWLARIDWDD 223
Query: 289 DSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
D +Y+ S+K R TIS+D S+N L M ++ DTA YYC
Sbjct: 224 DK-FYSTSLKTRLTISKDTSKNQVVLTMTNMDPVDTATYYC 263
>ref|XP_001718156.4| PREDICTED: ig heavy chain V-III region VH26-like [Homo sapiens].
Length = 148
Score = 169 bits (428), Expect = 4e-42
Identities = 86/124 (69%), Positives = 96/124 (77%), Gaps = 4/124 (3%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEF L+WV L A+LQGV E +LVESG GL QPGG L+LS SGFT S+ M W+ QAP
Sbjct: 1 MEFELSWVFLVAILQGVHCEVQLVESGRGLAQPGGYLKLSGAASGFTVGSWYMSWIHQAP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC----L 414
GKGLEW++YISSSG ST YADSVKGRFTIS DNS+NT YLQMNSLR EDTA YYC +
Sbjct: 61 GKGLEWVSYISSSGCSTNYADSVKGRFTISTDNSKNTLYLQMNSLRVEDTAVYYCARDTV 120
Query: 415 GGGH 426
GGH
Sbjct: 121 RGGH 124
>ref|XP_003119639.1| PREDICTED: hypothetical protein LOC100508797 [Homo sapiens].
Length = 309
Score = 167 bits (422), Expect = 2e-41
Identities = 88/138 (63%), Positives = 102/138 (73%), Gaps = 4/138 (2%)
Frame = +1
Query: 10 RSPAPGS----RSVIRKEHRPPAMEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSL 177
RSP+ G SVIR EH EF L+WV L A+L+ VQ E++LVESGGGLVQPGGSL
Sbjct: 108 RSPSLGIPKCLHSVIRTEHTGLTREFVLSWVFLVAILKCVQCEDQLVESGGGLVQPGGSL 167
Query: 178 RLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNT 357
R SC SGF FSSY + WVR+APGKG EW++ I + GD TYYADSV GRFTISRDN++ +
Sbjct: 168 RPSCAASGFAFSSYVLHWVRRAPGKGPEWVSAIGTGGD-TYYADSVMGRFTISRDNAKKS 226
Query: 358 AYLQMNSLRTEDTARYYC 411
YLQMNSL ED A YYC
Sbjct: 227 LYLQMNSLIAEDMAVYYC 244
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 50,679,253
Number of extensions: 1674961
Number of successful extensions: 8580
Number of sequences better than 1.0e-05: 21
Number of HSP's gapped: 8490
Number of HSP's successfully gapped: 24
Length of query: 346
Length of database: 18,297,164
Length adjustment: 103
Effective length of query: 243
Effective length of database: 14,901,872
Effective search space: 3621154896
Effective search space used: 3621154896
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000299
(1039 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_001472141.2| PREDICTED: hypothetical protein LOC16061 [Mu... 134 2e-31
Alignment gi|XP_003085513.1| PREDICTED: ig alpha chain C region [Mus musc... 127 1e-29
Alignment gi|XP_001474075.2| PREDICTED: ig alpha chain C region isoform 1... 127 1e-29
Alignment gi|XP_001478032.2| PREDICTED: hypothetical protein LOC100047361... 113 2e-25
Alignment gi|XP_003086829.1| PREDICTED: similar to immunoglobulin mu-chai... 108 9e-24
Alignment gi|XP_923669.1| PREDICTED: putative V-set and immunoglobulin do... 107 2e-23
Alignment gi|XP_003086828.1| PREDICTED: hypothetical protein LOC100505002... 103 3e-22
Alignment gi|XP_001474061.2| PREDICTED: hypothetical protein LOC100045292... 98 1e-20
Alignment gi|NP_058679.1| immunoglobulin omega chain precursor [Mus muscu... 53 3e-07
Alignment gi|NP_058678.1| immunoglobulin iota chain precursor [Mus muscul... 53 4e-07
>ref|XP_001472141.2| PREDICTED: hypothetical protein LOC16061 [Mus musculus].
Length = 349
Score = 134 bits (336), Expect = 2e-31
Identities = 74/160 (46%), Positives = 95/160 (59%), Gaps = 3/160 (1%)
Frame = +1
Query: 475 SPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRNFPPAQA-GGLYT 651
+P I+PLTL + + V+I CL+ D+FPS + VTW S + + NFPPA A GG YT
Sbjct: 10 NPTIYPLTLPRALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGGYT 69
Query: 652 MSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPCK--VLPSDPCPQCCKPSLSLQPPA 825
MSSQLTLP +CP + +KC VQH S + Q +NV C P+ P P C+PSLSLQ PA
Sbjct: 70 MSSQLTLPAVECPEGESVKCSVQHDSNAVQELNVKCSGPPPPTTPPPPSCQPSLSLQRPA 129
Query: 826 LADLLLGSMPASTAHSVA*KNPRVSASPGSPQVGKMPSQR 945
L DLLLGS + T +NP + P GK Q+
Sbjct: 130 LEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQK 169
Score = 93.6 bits (231), Expect = 2e-19
Identities = 47/104 (45%), Positives = 59/104 (56%), Gaps = 9/104 (8%)
Frame = +3
Query: 753 ALQSFTFRSMSPVLQAQPVPAATSPC----RPAP-----GLHASLNCTLSGLKKSEGVSF 905
A+Q + P P P + P RPA G AS+ CTL+GL+ EG F
Sbjct: 97 AVQELNVKCSGPPPPTTPPPPSCQPSLSLQRPALEDLLLGSDASITCTLNGLRNPEGAVF 156
Query: 906 TWQPSGGKDAVPASPTRDSCGCYSVSSILPGCADPWNKEKTFSC 1037
TW+PS GKDAV ++SCGCYSVSS+LPGCA+ WN +F C
Sbjct: 157 TWEPSTGKDAVQKKAVQNSCGCYSVSSVLPGCAERWNSGASFKC 200
>ref|XP_003085513.1| PREDICTED: ig alpha chain C region [Mus musculus].
Length = 413
Score = 127 bits (320), Expect = 1e-29
Identities = 99/297 (33%), Positives = 130/297 (43%), Gaps = 4/297 (1%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
M+ LNWV L LL G+Q E KL V+ GG L P
Sbjct: 1 MKLWLNWVFLLTLLHGIQCEVKL-------VESGGGL--------------------VQP 33
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGGH 426
G L S S +Y + V+ + + ++ S R
Sbjct: 34 GGSLRLSCATSGFTFSDFYMEWVR------QPPGKRLEWIAAKSAR-------------- 73
Query: 427 LWGPGXXXXXXXXXXTSPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSPSREGV 606
+P I+PLTL + + V+I CL+ D+FPS + VTW S + +
Sbjct: 74 ----------------NPTIYPLTLPPALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDI 117
Query: 607 IVRNFPPAQA-GGLYTMSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPCKVLPSDPC 783
NFPPA A GG YTMSSQLTLP +CP + +KC VQH S Q ++V C P+ P
Sbjct: 118 TTVNFPPALASGGRYTMSSQLTLPAVECPEGESVKCSVQHDSNPVQELDVNCSG-PTPPP 176
Query: 784 P---QCCKPSLSLQPPALADLLLGSMPASTAHSVA*KNPRVSASPGSPQVGKMPSQR 945
P C+PSLSLQ PAL DLLLGS + T +NP + P GK Q+
Sbjct: 177 PITIPSCQPSLSLQRPALEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQK 233
Score = 90.9 bits (224), Expect = 1e-18
Identities = 42/90 (46%), Positives = 53/90 (58%), Gaps = 12/90 (13%)
Frame = +3
Query: 804 PVPAATSPCRPAPGLH------------ASLNCTLSGLKKSEGVSFTWQPSGGKDAVPAS 947
P P C+P+ L AS+ CTL+GL+ EG FTW+PS GKDAV
Sbjct: 175 PPPITIPSCQPSLSLQRPALEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQKK 234
Query: 948 PTRDSCGCYSVSSILPGCADPWNKEKTFSC 1037
++SCGCYSVSS+LPGCA+ WN +F C
Sbjct: 235 AVQNSCGCYSVSSVLPGCAERWNSGASFKC 264
>ref|XP_001474075.2| PREDICTED: ig alpha chain C region isoform 1 [Mus musculus].
Length = 354
Score = 127 bits (319), Expect = 1e-29
Identities = 73/161 (45%), Positives = 94/161 (58%), Gaps = 4/161 (2%)
Frame = +1
Query: 475 SPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSPSREGVIVRNFPPAQA-GGLYT 651
+P I+PLTL + + V+I CL+ D+FPS + VTW S + + NFPPA A GG YT
Sbjct: 15 NPTIYPLTLPPALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGRYT 74
Query: 652 MSSQLTLPVEQCPADQILKCQVQHLSKSSQSVNVPCKVLPSDPCP---QCCKPSLSLQPP 822
MSSQLTLP +CP + +KC VQH S Q ++V C P+ P P C+PSLSLQ P
Sbjct: 75 MSSQLTLPAVECPEGESVKCSVQHDSNPVQELDVNCSG-PTPPPPITIPSCQPSLSLQRP 133
Query: 823 ALADLLLGSMPASTAHSVA*KNPRVSASPGSPQVGKMPSQR 945
AL DLLLGS + T +NP + P GK Q+
Sbjct: 134 ALEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQK 174
Score = 90.9 bits (224), Expect = 1e-18
Identities = 42/90 (46%), Positives = 53/90 (58%), Gaps = 12/90 (13%)
Frame = +3
Query: 804 PVPAATSPCRPAPGLH------------ASLNCTLSGLKKSEGVSFTWQPSGGKDAVPAS 947
P P C+P+ L AS+ CTL+GL+ EG FTW+PS GKDAV
Sbjct: 116 PPPITIPSCQPSLSLQRPALEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQKK 175
Query: 948 PTRDSCGCYSVSSILPGCADPWNKEKTFSC 1037
++SCGCYSVSS+LPGCA+ WN +F C
Sbjct: 176 AVQNSCGCYSVSSVLPGCAERWNSGASFKC 205
>ref|XP_001478032.2| PREDICTED: hypothetical protein LOC100047361 [Mus musculus].
Length = 411
Score = 113 bits (283), Expect = 2e-25
Identities = 55/103 (53%), Positives = 73/103 (70%)
Frame = +1
Query: 103 LLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISS 282
LL G+ + +L ESG GLV P SL ++C SGF+ + Y + WVRQ PGKGLEWL I
Sbjct: 253 LLPGILSQVQLKESGPGLVAPSQSLSITCTVSGFSLTGYGVNWVRQPPGKGLEWLGMIWG 312
Query: 283 SGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
G ST Y ++K R +IS+DNS++ +L+MNSL+T+DTARYYC
Sbjct: 313 DG-STDYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTARYYC 354
Score = 84.7 bits (208), Expect = 1e-16
Identities = 43/94 (45%), Positives = 62/94 (65%)
Frame = +1
Query: 115 VQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGDS 294
V + ++ ESG LVQP +L L+C SGF+ SSY + W R+ P KGLEWL I S G S
Sbjct: 17 VLSQVQMKESGPDLVQPSQTLSLTCTVSGFSLSSYGVHWFRKPPRKGLEWLGGIWSGG-S 75
Query: 295 TYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDT 396
YY ++ R ++SRD S++ + +M+SL++EDT
Sbjct: 76 IYYNPALSSRLSVSRDISKSQVFFKMSSLQSEDT 109
>ref|XP_003086829.1| PREDICTED: similar to immunoglobulin mu-chain-like [Mus musculus].
Length = 124
Score = 108 bits (269), Expect = 9e-24
Identities = 50/112 (44%), Positives = 75/112 (66%), Gaps = 2/112 (1%)
Frame = +1
Query: 82 NWVVLFAL--LQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKG 255
+W+ LF L GV E +L +SG LV+PG S+++SC SG+TF++Y M WV+Q+ GK
Sbjct: 4 SWIFLFLLSGTAGVLSEVQLHQSGPELVKPGASVKMSCKASGYTFTNYYMHWVKQSHGKS 63
Query: 256 LEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
+EW+ YI + T Y KG+ T++ D S +TAY+++ SL +ED+A YYC
Sbjct: 64 IEWIGYIYPNNGDTSYNQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYC 115
>ref|XP_923669.1| PREDICTED: putative V-set and immunoglobulin domain-containing
protein 6-like [Mus musculus].
Length = 208
Score = 107 bits (267), Expect = 2e-23
Identities = 54/109 (49%), Positives = 74/109 (67%)
Frame = +1
Query: 85 WVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEW 264
++ L A V + +L ESG GLV P SL ++C SGF+ +SY + WVRQ PGKGLEW
Sbjct: 7 FLCLVAFPSCVLSQVQLKESGPGLVAPSQSLSITCTVSGFSLTSYGVHWVRQPPGKGLEW 66
Query: 265 LTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
L I + G ST Y ++ R +IS+DNS++ +L+MNSL+T+DTA YYC
Sbjct: 67 LGVIWAGG-STNYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYYC 114
>ref|XP_003086828.1| PREDICTED: hypothetical protein LOC100505002 [Mus musculus].
Length = 302
Score = 103 bits (256), Expect = 3e-22
Identities = 48/112 (42%), Positives = 75/112 (66%), Gaps = 2/112 (1%)
Frame = +1
Query: 82 NWVVLFAL--LQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKG 255
+W+ LF L GV E +L +SG L++PG S+++SC SG+TF+ Y+M WV+Q+ GK
Sbjct: 4 SWIFLFLLSGTAGVLSEVQLQQSGPELLKPGASVKISCKASGYTFTDYTMHWVKQSHGKS 63
Query: 256 LEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
LEW+ I+ + T Y + K + T++ D S +TAY+++ SL +ED+A YYC
Sbjct: 64 LEWIGGINPNYGGTSYNEKFKDKATLTVDKSSSTAYMELRSLTSEDSAVYYC 115
Score = 77.8 bits (190), Expect = 1e-14
Identities = 38/100 (38%), Positives = 62/100 (62%)
Frame = +1
Query: 112 GVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGD 291
GV E L +S +V+ G S+++SC SG++F+ Y M WV+Q+ GK L+ + YI+
Sbjct: 179 GVHSEVHLQQSLPKVVKAGPSVKISCKASGYSFTGYYMHWVKQSHGKILQRVEYINPYNG 238
Query: 292 STYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
T Y + K + T++ D S +TAY+ ++SL +ED+ Y C
Sbjct: 239 GTGYIEKFKDKATLTADKSFSTAYMHLSSLTSEDSEVYSC 278
Score = 62.0 bits (149), Expect = 7e-10
Identities = 27/63 (42%), Positives = 41/63 (65%)
Frame = +1
Query: 91 VLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLT 270
V + V E +L +SG LV+PG S+++SC SG++F+ Y M WV+Q+ GK LEW+
Sbjct: 112 VYYCARHSVNSEVQLQQSGPELVKPGASVKISCKASGYSFTGYYMHWVKQSHGKSLEWIG 171
Query: 271 YIS 279
I+
Sbjct: 172 EIN 174
>ref|XP_001474061.2| PREDICTED: hypothetical protein LOC100045292 [Mus musculus].
Length = 419
Score = 97.8 bits (242), Expect = 1e-20
Identities = 47/106 (44%), Positives = 67/106 (63%), Gaps = 3/106 (2%)
Frame = +1
Query: 103 LLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTYISS 282
LL G + +L +SG LV+PG S+++SC SG+TF+SY M WV+Q PG+GLEW+ I
Sbjct: 4 LLAGFNSQVQLQQSGAELVRPGASVKMSCKASGYTFTSYWMHWVKQRPGQGLEWIGAIDP 63
Query: 283 SGDSTYYADSVK---GRFTISRDNSQNTAYLQMNSLRTEDTARYYC 411
S T Y K + T++ D S + AY+Q +SL +ED+ YYC
Sbjct: 64 SDSETIYNQKFKDSRDKATLTVDKSSSIAYMQFSSLTSEDSVVYYC 109
>ref|NP_058679.1| immunoglobulin omega chain precursor [Mus musculus].
Length = 142
Score = 53.1 bits (126), Expect = 3e-07
Identities = 33/112 (29%), Positives = 54/112 (48%), Gaps = 1/112 (0%)
Frame = +1
Query: 88 VVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSC-VGSGFTFSSYSMRWVRQAPGKGLEW 264
++L A L G + + + G ++RLSC + + YS+ W +Q PG +
Sbjct: 8 LMLLAHLTGCGPQPMVHQPPSASSSLGATIRLSCTLSNDHNIGIYSIYWYQQRPGHPPRF 67
Query: 265 LTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGG 420
L S D + + RF+ S+D ++N YL ++ L+ ED A YYC G
Sbjct: 68 LLRYFSHSDK-HQGPDIPPRFSGSKDTARNLGYLSISELQPEDEAVYYCAVG 118
>ref|NP_058678.1| immunoglobulin iota chain precursor [Mus musculus].
Length = 142
Score = 52.8 bits (125), Expect = 4e-07
Identities = 29/86 (33%), Positives = 45/86 (52%), Gaps = 1/86 (1%)
Frame = +1
Query: 166 GGSLRLSC-VGSGFTFSSYSMRWVRQAPGKGLEWLTYISSSGDSTYYADSVKGRFTISRD 342
G ++RLSC + + YS+ W +Q PG +L S D + + RF+ S+D
Sbjct: 34 GATIRLSCTLSNDHNIGIYSIYWYQQRPGHPPRFLLRYFSHSDK-HQGPDIPPRFSGSKD 92
Query: 343 NSQNTAYLQMNSLRTEDTARYYCLGG 420
++N YL ++ L+ ED A YYC G
Sbjct: 93 TTRNLGYLSISELQPEDEAVYYCAVG 118
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 42,658,247
Number of extensions: 1390866
Number of successful extensions: 6604
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 6517
Number of HSP's successfully gapped: 17
Length of query: 346
Length of database: 15,617,559
Length adjustment: 102
Effective length of query: 244
Effective length of database: 12,553,887
Effective search space: 3063148428
Effective search space used: 3063148428
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000299
(1039 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_998993.1| IgG heavy chain [Sus scrofa]. 252 6e-68
Alignment gi|XP_003359207.1| PREDICTED: immunoglobulin lambda-like polype... 75 8e-14
Alignment gi|XP_003359212.1| PREDICTED: ig lambda chain V-III region SH-l... 56 4e-08
>ref|NP_998993.1| IgG heavy chain [Sus scrofa].
Length = 474
Score = 252 bits (644), Expect(2) = 6e-68
Identities = 147/280 (52%), Positives = 174/280 (62%), Gaps = 21/280 (7%)
Frame = +1
Query: 67 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAP 246
MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSL+LSCV SGF FS Y + WVR P
Sbjct: 1 MEFRLNWVVLFALLQGVQGEEKLVESGGGLVQPGGSLKLSCVASGFIFSKYIVNWVRLVP 60
Query: 247 GKGLEWLTYISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLG--- 417
GKGLEWL I S+G ST+YADSVKGRFTISRDNSQNTAYLQMNSLRTEDTA YYC
Sbjct: 61 GKGLEWLASIWSTG-STHYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTALYYCAREVC 119
Query: 418 ------------GGHLWGPGXXXXXXXXXXTSPKIFPLTLGSSEPAG-YVVIACLVRDFF 558
G LWGPG T+P ++PL + +G V + CL +F
Sbjct: 120 FRYNTGCWDGYYGMDLWGPGVEVVVSSAPKTAPSVYPLAPCGRDTSGPNVALGCLASSYF 179
Query: 559 PSEPLTVTWSPSREGVIVRNFPPA-QAGGLYTMSSQLTLPVEQCPADQILKCQVQHLSKS 735
P EP+T+TW+ V FP Q GLY++SS +T+P + + C V H + +
Sbjct: 180 P-EPVTMTWNSGALTSGVHTFPSVLQPSGLYSLSSMVTVPASSL-SSKSYTCNVNHPATT 237
Query: 736 SQ-SVNVPCKVLPSDP-CPQC--CKPSLSLQPPALADLLL 843
++ V K P P CP C PS+ + PP D L+
Sbjct: 238 TKVDKRVGTKTKPPCPICPGCEVAGPSVFIFPPKPKDTLM 277
Score = 23.9 bits (50), Expect(2) = 6e-68
Identities = 18/56 (32%), Positives = 23/56 (41%), Gaps = 4/56 (7%)
Frame = +3
Query: 882 KKSEGVSFTWQPSGGKDAVPASPTRDS----CGCYSVSSILPGCADPWNKEKTFSC 1037
K+ V F+W G + V + TR Y V S+LP W K K F C
Sbjct: 293 KEHAEVQFSWYVDGVE--VHTAETRPKEEQFNSTYRVVSVLPIQHQDWLKGKEFKC 346
>ref|XP_003359207.1| PREDICTED: immunoglobulin lambda-like polypeptide 5-like [Sus
scrofa].
Length = 233
Score = 74.7 bits (182), Expect = 8e-14
Identities = 65/222 (29%), Positives = 91/222 (40%), Gaps = 12/222 (5%)
Frame = +1
Query: 94 LFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTY 273
L +L G +L + G V GG+ ++C G F SY W +Q PG+ + Y
Sbjct: 10 LLSLCTGYVASSQLTQPPGVSVSLGGTASITCQGGNF--GSYYAHWYKQKPGQSPMLVIY 67
Query: 274 ISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLG-----GGHLWGP 438
+ A + RF+ S+ S NTA L ++ + ED A YYC G L+G
Sbjct: 68 -----EYNKRASGIPDRFSGSK--SGNTATLTISGAQAEDEADYYCQSSDDSDAGPLFGR 120
Query: 439 GXXXXXXXXXXTSP--KIFPLTLGSSEPAG--YVVIACLVRDFFPSEPLTVTWSPSREGV 606
G +P +FP SSE G + CL+ DF+P +TVTW V
Sbjct: 121 GTRLSVLRQPKAAPTVNLFP---PSSEELGTNKATLVCLISDFYPG-AVTVTWKAGGTTV 176
Query: 607 ---IVRNFPPAQAGGLYTMSSQLTLPVEQCPADQILKCQVQH 723
+ P Q+ Y SS L L + CQV H
Sbjct: 177 TQGVETTKPSKQSNNKYAASSYLALSASDWKSSSGFTCQVTH 218
>ref|XP_003359212.1| PREDICTED: ig lambda chain V-III region SH-like [Sus scrofa].
Length = 179
Score = 55.8 bits (133), Expect = 4e-08
Identities = 50/175 (28%), Positives = 72/175 (41%), Gaps = 4/175 (2%)
Frame = +1
Query: 94 LFALLQGVQGEEKLVESGGGLVQPGGSLRLSCVGSGFTFSSYSMRWVRQAPGKGLEWLTY 273
L +L G KL + G V GG+ ++C G F SY W +Q PG+ + Y
Sbjct: 10 LLSLCTGYVASSKLTQPPGVSVSLGGTASIACQGDNF--GSYYAHWYQQKPGQSPMLVIY 67
Query: 274 ISSSGDSTYYADSVKGRFTISRDNSQNTAYLQMNSLRTEDTARYYCLGGGHLWGP-GXXX 450
+ A + RF+ S+ S NTA L ++ + ED A YYC + P
Sbjct: 68 -----EYNKRASGIPDRFSGSK--SGNTATLTISGAQAEDEADYYCQSSDDSYTPHSDTG 120
Query: 451 XXXXXXXTSPKIFPLTLGSSEPAGYVVIACLVRDFFPSEPLTVTWSP---SREGV 606
T P++ L L E G CL+ S P+ + SP S +GV
Sbjct: 121 GGGRGTQTPPQLGHLLLQHQEACGQSKNRCLLGSTDLSSPIFPSPSPQAGSAQGV 175
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 32,145,387
Number of extensions: 1078314
Number of successful extensions: 5566
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 5508
Number of HSP's successfully gapped: 4
Length of query: 346
Length of database: 11,343,932
Length adjustment: 100
Effective length of query: 246
Effective length of database: 8,854,232
Effective search space: 2178141072
Effective search space used: 2178141072
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000299
(1039 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GL896422.1| Sus scrofa unplaced genomic scaffold chrU_scaffol... 595 e-167
gb|GL896114.1| Sus scrofa unplaced genomic scaffold chrU_scaffol... 280 1e-72
>gb|GL896422.1| Sus scrofa unplaced genomic scaffold chrU_scaffold764
Length = 7672
Score = 595 bits (300), Expect = e-167
Identities = 303/304 (99%)
Strand = Plus / Plus
Query: 460 tcagtgtctgaaaccagccccaaaatcttcccactgactctggggagcagcgagcctgcc 519
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1885 tcagtgtctgaaaccagccccaaaatcttcccactgactctggggagcagcgagcctgcc 1944
Query: 520 ggatatgtggtcatcgcctgcctggtccgggacttcttcccgtcagagcccctgacagta 579
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1945 ggatatgtggtcatcgcctgcctggtccgggacttcttcccgtcagagcccctgacagta 2004
Query: 580 acctggagccctagtcgcgagggcgttattgtcagaaacttcccccctgcgcaggccggg 639
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2005 acctggagccctagtcgcgagggcgttattgtcagaaacttcccccctgcgcaggccggg 2064
Query: 640 ggcctgtacaccatgagcagccagctgaccttgccagtcgagcagtgcccagccgatcag 699
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 2065 ggcctgtacaccatgagcagccaggtgaccttgccagtcgagcagtgcccagccgatcag 2124
Query: 700 atcctgaagtgccaagtgcagcacctctccaaatccagccagtccgtgaacgtgccctgc 759
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2125 atcctgaagtgccaagtgcagcacctctccaaatccagccagtccgtgaacgtgccctgc 2184
Query: 760 aaag 763
||||
Sbjct: 2185 aaag 2188
Score = 474 bits (239), Expect = e-131
Identities = 270/279 (96%), Gaps = 1/279 (0%)
Strand = Plus / Plus
Query: 762 agttttaccttcagatccatgtccccagtgctgcaagcccagcctgtccctgcagccacc 821
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2334 agttttaccttcagatccatgtccccagtgctgcaagcccagcctgtccctgcagccacc 2393
Query: 822 agcccttgcagacctgctcctgggctcca-tgccagcctcaactgcacactcagtggcct 880
||||||||||||||||||||||||||||| ||||||||||| ||||||||||||||||||
Sbjct: 2394 agcccttgcagacctgctcctgggctccaatgccagcctcacctgcacactcagtggcct 2453
Query: 881 gaaaaaatccgagggtgtcagcttcacctggcagccctcaggtgggaaagatgccgtccc 940
|||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 2454 gaaaaaatccgagggtgtcagcttcacctggcagccctcaggtgggaaggatgccgtcca 2513
Query: 941 agcgtcccccacgcgtgattcctgtggctgctacagcgtgtccagcatcctgccaggctg 1000
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 2514 ggcgtcccccacgcgtgattcctgtggctgctacagcgtgtccagcatcctgccaggctg 2573
Query: 1001 tgctgatccttggaacaaagaaaaaacattctcttgcac 1039
|||||||||||||||||||| | | |||||||| |||||
Sbjct: 2574 tgctgatccttggaacaaaggagagacattctcctgcac 2612
>gb|GL896114.1| Sus scrofa unplaced genomic scaffold chrU_scaffold487
Length = 3028
Score = 280 bits (141), Expect = 1e-72
Identities = 159/165 (96%)
Strand = Plus / Minus
Query: 247 gggaaggggctggagtggctgacatatattagtagtagtggtgatagcacctattacgca 306
||||||||||||||||||||| ||||||||| ||||||||||| ||||||||| ||||||
Sbjct: 2633 gggaaggggctggagtggctggcatatattactagtagtggtggtagcacctactacgca 2574
Query: 307 gactctgtgaagggccgattcaccatctccagagacaactcccagaacacggcctatctg 366
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct: 2573 gactctgtgaagggccgattcaccatctccagcgacaactcccagaacacggcctatctg 2514
Query: 367 caaatgaacagcttgagaaccgaagacacggcccgctattactgt 411
|||||||||||| ||||||||||||||||||||||||||||||||
Sbjct: 2513 caaatgaacagcctgagaaccgaagacacggcccgctattactgt 2469
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 29,548,983
Number of extensions: 208
Number of successful extensions: 208
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 207
Number of HSP's successfully gapped: 3
Length of query: 1039
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 1018
Effective length of database: 2,808,413,156
Effective search space: 2858964592808
Effective search space used: 2858964592808
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)