Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000604
(1015 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001029471.1| phosphoglycerate kinase 1 [Bos taurus]. 476 e-134
Alignment gi|NP_001116507.1| phosphoglycerate kinase 2 [Bos taurus]. 442 e-129
>ref|NP_001029471.1| phosphoglycerate kinase 1 [Bos taurus].
Length = 417
Score = 476 bits (1224), Expect = e-134
Identities = 243/272 (89%), Positives = 257/272 (94%), Gaps = 2/272 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAA+PSIK+CLD+GAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKYCLDSGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL+PVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLQPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+P KIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAEPTKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLI+NMLDKVNEMIIGGGM
Sbjct: 181 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLISNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFSFDERDPDR--QDLMSK 933
AFTFLK + + S + + + +DLMSK
Sbjct: 241 AFTFLKVLNNMEIGTSLFDEEGSKIVKDLMSK 272
>ref|NP_001116507.1| phosphoglycerate kinase 2 [Bos taurus].
Length = 417
Score = 442 bits (1137), Expect(2) = e-129
Identities = 220/246 (89%), Positives = 236/246 (95%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLS KLTLDK+DVKGKRVVMRVDFNVPMK NQITNNQRIKA++PSIK+CLDNGAKSVVL
Sbjct: 1 MSLSKKLTLDKVDVKGKRVVMRVDFNVPMKKNQITNNQRIKASLPSIKYCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSLEPVA ELKSLLGKDV+FLKDCVGPEVEKA A PA G+VILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLEPVAAELKSLLGKDVVFLKDCVGPEVEKASASPATGTVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKG+D SG+K+KA+P KIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGQDPSGNKLKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KA GFLMKKEL+YF+KALE+PERPFLAILGGAKVADKIQLI NMLDKVNEMIIGGGM
Sbjct: 181 LPQKASGFLMKKELDYFSKALENPERPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGM 240
Query: 844 AFTFLK 861
A+TFLK
Sbjct: 241 AYTFLK 246
Score = 39.3 bits (90), Expect(2) = e-129
Identities = 24/53 (45%), Positives = 35/53 (66%), Gaps = 2/53 (3%)
Frame = +3
Query: 861 VLNTWRL-ALLF**EGSRSSRSDV-QAEKNGVKNTLPGDFVTAEQFDENAQIG 1013
VLN + A LF EG++ + + +A KN V T P DFVTA++F+ENA++G
Sbjct: 247 VLNNMEIGASLFDEEGAKIVKEIMTKAGKNSVNITFPVDFVTADKFEENAKVG 299
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 36,932,384
Number of extensions: 936946
Number of successful extensions: 2858
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 2856
Number of HSP's successfully gapped: 3
Length of query: 338
Length of database: 17,681,374
Length adjustment: 103
Effective length of query: 235
Effective length of database: 14,273,310
Effective search space: 3354227850
Effective search space used: 3354227850
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000604
(1015 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_860634.1| PREDICTED: similar to phosphoglycerate kinase 1... 465 e-138
Alignment gi|XP_860604.1| PREDICTED: similar to phosphoglycerate kinase 1... 465 e-136
Alignment gi|XP_860505.1| PREDICTED: similar to phosphoglycerate kinase 1... 449 e-133
Alignment gi|XP_860404.1| PREDICTED: similar to phosphoglycerate kinase 1... 468 e-132
Alignment gi|XP_849992.1| PREDICTED: similar to phosphoglycerate kinase 1... 468 e-132
Alignment gi|XP_532167.1| PREDICTED: similar to Phosphoglycerate kinase, ... 445 e-125
Alignment gi|XP_860472.1| PREDICTED: similar to phosphoglycerate kinase 1... 406 e-113
Alignment gi|XP_860570.1| PREDICTED: similar to phosphoglycerate kinase 1... 373 e-103
Alignment gi|XP_854345.1| PREDICTED: similar to phosphoglycerate kinase 1... 136 4e-32
Alignment gi|XP_860438.1| PREDICTED: similar to phosphoglycerate kinase 1... 115 5e-26
>ref|XP_860634.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 10 [Canis
familiaris].
Length = 423
Score = 465 bits (1196), Expect(2) = e-138
Identities = 236/246 (95%), Positives = 242/246 (98%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLGPVAVELKSLLGKDVLFLKDCVGQEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+ AKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGV+
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAESAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVS 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KAGGFLMKKELNYFAKALE PERPFLAILGGAKVADKIQLI+NMLDKVNEMIIGGGM
Sbjct: 181 LPQKAGGFLMKKELNYFAKALECPERPFLAILGGAKVADKIQLISNMLDKVNEMIIGGGM 240
Query: 844 AFTFLK 861
AFTFLK
Sbjct: 241 AFTFLK 246
Score = 45.1 bits (105), Expect(2) = e-138
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = +3
Query: 930 QAEKNGVKNTLPGDFVTAEQFDENAQIG 1013
+AEKNGVK TLP DFVTAE+FDE+A+ G
Sbjct: 278 KAEKNGVKITLPVDFVTAEKFDEHAKTG 305
>ref|XP_860604.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 9 [Canis
familiaris].
Length = 407
Score = 465 bits (1196), Expect(2) = e-136
Identities = 236/246 (95%), Positives = 242/246 (98%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLGPVAVELKSLLGKDVLFLKDCVGQEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+ AKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGV+
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAESAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVS 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KAGGFLMKKELNYFAKALE PERPFLAILGGAKVADKIQLI+NMLDKVNEMIIGGGM
Sbjct: 181 LPQKAGGFLMKKELNYFAKALECPERPFLAILGGAKVADKIQLISNMLDKVNEMIIGGGM 240
Query: 844 AFTFLK 861
AFTFLK
Sbjct: 241 AFTFLK 246
Score = 40.0 bits (92), Expect(2) = e-136
Identities = 19/27 (70%), Positives = 23/27 (85%)
Frame = +3
Query: 933 AEKNGVKNTLPGDFVTAEQFDENAQIG 1013
A+ NGVK TLP DFVTAE+FDE+A+ G
Sbjct: 263 AKINGVKITLPVDFVTAEKFDEHAKTG 289
>ref|XP_860505.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 6 [Canis
familiaris].
Length = 412
Score = 449 bits (1154), Expect(2) = e-133
Identities = 240/281 (85%), Positives = 248/281 (88%), Gaps = 8/281 (2%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLGPVAVELKSLLGKDVLFLKDCVGQEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSK-------VKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAH 642
NLRFHVEEEGKGKDASG+K VKA+ AKIEAFRASLSKLGDVYVNDAFGTAHRAH
Sbjct: 121 NLRFHVEEEGKGKDASGNKTNEASQNVKAESAKIEAFRASLSKLGDVYVNDAFGTAHRAH 180
Query: 643 SSMVGVNLPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNE 822
SSMVGV+LP+KAGGFLMKKELNYFAKALE PERPFLAILGGAKVADKIQLI+NMLDKVNE
Sbjct: 181 SSMVGVSLPQKAGGFLMKKELNYFAKALECPERPFLAILGGAKVADKIQLISNMLDKVNE 240
Query: 823 MIIGGGMAFTFLKCSTHGDWHFSFDERDPD-RQDLMSKLRR 942
MIIGGGM T L FDE +DLMSK +
Sbjct: 241 MIIGGGMIGTSL-----------FDEEGAKIVKDLMSKAEK 270
Score = 45.1 bits (105), Expect(2) = e-133
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = +3
Query: 930 QAEKNGVKNTLPGDFVTAEQFDENAQIG 1013
+AEKNGVK TLP DFVTAE+FDE+A+ G
Sbjct: 267 KAEKNGVKITLPVDFVTAEKFDEHAKTG 294
>ref|XP_860404.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 3 [Canis
familiaris].
Length = 382
Score = 468 bits (1203), Expect = e-132
Identities = 245/275 (89%), Positives = 255/275 (92%), Gaps = 2/275 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLGPVAVELKSLLGKDVLFLKDCVGQEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+ AKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGV+
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAESAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVS 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KAGGFLMKKELNYFAKALE PERPFLAILGGAKVADKIQLI+NMLDKVNEMIIGGGM
Sbjct: 181 LPQKAGGFLMKKELNYFAKALECPERPFLAILGGAKVADKIQLISNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFS-FDERDPD-RQDLMSKLRR 942
AFTFLK + + S FDE +DLMSK +
Sbjct: 241 AFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEK 275
>ref|XP_849992.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 2 [Canis
familiaris].
Length = 417
Score = 468 bits (1203), Expect = e-132
Identities = 245/275 (89%), Positives = 255/275 (92%), Gaps = 2/275 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLGPVAVELKSLLGKDVLFLKDCVGQEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+ AKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGV+
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAESAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVS 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KAGGFLMKKELNYFAKALE PERPFLAILGGAKVADKIQLI+NMLDKVNEMIIGGGM
Sbjct: 181 LPQKAGGFLMKKELNYFAKALECPERPFLAILGGAKVADKIQLISNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFS-FDERDPD-RQDLMSKLRR 942
AFTFLK + + S FDE +DLMSK +
Sbjct: 241 AFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEK 275
>ref|XP_532167.1| PREDICTED: similar to Phosphoglycerate kinase, testis specific
[Canis familiaris].
Length = 417
Score = 445 bits (1145), Expect = e-125
Identities = 228/274 (83%), Positives = 248/274 (90%), Gaps = 2/274 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLS KLTLDKLDVKGKRV+MRVDFNVPMK NQITNNQRIKA+IPSI +CLDNGA+SVVL
Sbjct: 1 MSLSKKLTLDKLDVKGKRVIMRVDFNVPMKKNQITNNQRIKASIPSITYCLDNGARSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSLEPVA ELKSLL KDVLFL DCVGPEVEKACA+PA GSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLEPVAAELKSLLSKDVLFLNDCVGPEVEKACANPATGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKG+D+SG+K+KA+P KIE FRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGQDSSGNKLKAEPDKIEGFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KA GFLMKKEL+YFA+ALE+PERPFLAILGGAKVADKIQLI NMLDKVNEMIIGGGM
Sbjct: 181 LPQKASGFLMKKELDYFARALENPERPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFS-FDERDPD-RQDLMSKLR 939
AFTFLK + D S FDE +D+M+K +
Sbjct: 241 AFTFLKVLNNMDIGASLFDEEGAKIVKDIMAKAK 274
>ref|XP_860472.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 5 [Canis
familiaris].
Length = 229
Score = 406 bits (1043), Expect = e-113
Identities = 205/214 (95%), Positives = 210/214 (98%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLGPVAVELKSLLGKDVLFLKDCVGQEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+ AKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGV+
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAESAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVS 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGG 765
LP+KAGGFLMKKELNYFAKALE PERPFLAILGG
Sbjct: 181 LPQKAGGFLMKKELNYFAKALECPERPFLAILGG 214
>ref|XP_860570.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 8 [Canis
familiaris].
Length = 377
Score = 373 bits (958), Expect = e-103
Identities = 207/275 (75%), Positives = 215/275 (78%), Gaps = 2/275 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLGPVAVELKSLLGKDVLFLKDCVGQEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+ AKIEAFRASLSKLGDVYVNDAFGTAHRAH
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAESAKIEAFRASLSKLGDVYVNDAFGTAHRAHR------ 174
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
AKVADKIQLI+NMLDKVNEMIIGGGM
Sbjct: 175 ----------------------------------AKVADKIQLISNMLDKVNEMIIGGGM 200
Query: 844 AFTFLKCSTHGDWHFS-FDERDPD-RQDLMSKLRR 942
AFTFLK + + S FDE +DLMSK +
Sbjct: 201 AFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEK 235
>ref|XP_854345.1| PREDICTED: similar to phosphoglycerate kinase 1 [Canis familiaris].
Length = 184
Score = 136 bits (342), Expect = 4e-32
Identities = 79/124 (63%), Positives = 88/124 (70%), Gaps = 2/124 (1%)
Frame = +1
Query: 649 MVGVNLPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMI 828
MVGV+LP+KAGGFLMKKELNYFAKALE PERP LAILGGAKVADKIQLI+NMLDKVNEMI
Sbjct: 1 MVGVSLPQKAGGFLMKKELNYFAKALECPERPILAILGGAKVADKIQLISNMLDKVNEMI 60
Query: 829 IGGGMAFTFLKCSTHGDWHFS-FDERDPD-RQDLMSKLRRMV*RIXXXXXXXXXXXXMRM 1002
IGGGMAFTFLK + + FDE +DLMSK + ++ M M
Sbjct: 61 IGGGMAFTFLKVLNNMEIGTPLFDEEGAKIAKDLMSKAEKNGVKLPCQLTWSLLKSLMSM 120
Query: 1003 PRLA 1014
P LA
Sbjct: 121 PTLA 124
>ref|XP_860438.1| PREDICTED: similar to phosphoglycerate kinase 1 isoform 4 [Canis
familiaris].
Length = 59
Score = 115 bits (289), Expect = 5e-26
Identities = 57/59 (96%), Positives = 59/59 (100%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVV 300
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKS++
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSLL 59
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 38,641,617
Number of extensions: 968936
Number of successful extensions: 3004
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 3002
Number of HSP's successfully gapped: 14
Length of query: 338
Length of database: 18,874,504
Length adjustment: 103
Effective length of query: 235
Effective length of database: 15,440,896
Effective search space: 3628610560
Effective search space used: 3628610560
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000604
(1015 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_000282.1| phosphoglycerate kinase 1 [Homo sapiens]. 480 e-135
Alignment gi|NP_620061.2| phosphoglycerate kinase 2 [Homo sapiens]. 443 e-124
>ref|NP_000282.1| phosphoglycerate kinase 1 [Homo sapiens].
Length = 417
Score = 480 bits (1235), Expect = e-135
Identities = 249/275 (90%), Positives = 259/275 (94%), Gaps = 2/275 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAA+PSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACA+PAAGSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+PAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM
Sbjct: 181 LPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFS-FDERDPD-RQDLMSKLRR 942
AFTFLK + + S FDE +DLMSK +
Sbjct: 241 AFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEK 275
>ref|NP_620061.2| phosphoglycerate kinase 2 [Homo sapiens].
Length = 417
Score = 443 bits (1139), Expect = e-124
Identities = 228/275 (82%), Positives = 248/275 (90%), Gaps = 2/275 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLS KLTLDKLDV+GKRV+MRVDFNVPMK NQITNNQRIKA+IPSIK+CLDNGAK+VVL
Sbjct: 1 MSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSL PVAVELKSLLGKDVLFLKDCVG EVEKACA+PA GSVILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKG+D SG K+KA+P KIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP KA GFLMKKEL+YFAKALE+P RPFLAILGGAKVADKIQLI NMLDKVNEMIIGGGM
Sbjct: 181 LPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFS-FDERDPD-RQDLMSKLRR 942
A+TFLK + + S FDE +D+M+K ++
Sbjct: 241 AYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQK 275
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 37,926,441
Number of extensions: 953139
Number of successful extensions: 3064
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 3059
Number of HSP's successfully gapped: 2
Length of query: 338
Length of database: 18,297,164
Length adjustment: 103
Effective length of query: 235
Effective length of database: 14,901,872
Effective search space: 3501939920
Effective search space used: 3501939920
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000604
(1015 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_032854.2| phosphoglycerate kinase 1 [Mus musculus]. 474 e-141
Alignment gi|NP_112467.2| phosphoglycerate kinase 2 [Mus musculus]. 434 e-128
>ref|NP_032854.2| phosphoglycerate kinase 1 [Mus musculus].
Length = 417
Score = 474 bits (1219), Expect(2) = e-141
Identities = 242/275 (88%), Positives = 257/275 (93%), Gaps = 2/275 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAA+PSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+PMPDKYSLEPVA ELKSLLGKDVLFLKDCVGPEVE ACA+PAAG+VILLE
Sbjct: 61 MSHLGRPDGVPMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASG+KVKA+PAKI+AFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGKDASGNKVKAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM
Sbjct: 181 LPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFSFDERDPDR--QDLMSKLRR 942
AFTFLK + + S + + + +DLMSK +
Sbjct: 241 AFTFLKVLNNMEIGTSLYDEEGAKIVKDLMSKAEK 275
Score = 45.8 bits (107), Expect(2) = e-141
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = +3
Query: 930 QAEKNGVKNTLPGDFVTAEQFDENAQIG 1013
+AEKNGVK TLP DFVTA++FDENA+ G
Sbjct: 272 KAEKNGVKITLPVDFVTADKFDENAKTG 299
>ref|NP_112467.2| phosphoglycerate kinase 2 [Mus musculus].
Length = 417
Score = 434 bits (1116), Expect(2) = e-128
Identities = 216/246 (87%), Positives = 232/246 (94%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
M+LS KLTLDK+D+KGKRV+MRVDFNVPMKNNQITNNQRIKAAIPSIK CLDNGAKSVVL
Sbjct: 1 MALSAKLTLDKVDLKGKRVIMRVDFNVPMKNNQITNNQRIKAAIPSIKHCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDGIPMPDKYSLEPVA ELKSLL KDV+FLKDCVGPEVE+ACA+P GS+ILLE
Sbjct: 61 MSHLGRPDGIPMPDKYSLEPVADELKSLLNKDVIFLKDCVGPEVEQACANPDNGSIILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKD+SG K+ ADPAK+EAF+ASLSKLGDVYVNDAFGTAHRAHSS VGVN
Sbjct: 121 NLRFHVEEEGKGKDSSGKKISADPAKVEAFQASLSKLGDVYVNDAFGTAHRAHSSTVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KA GFLMKKEL+YF+KALE PERPFLAILGGAKV DKIQLI NMLDKVN MIIGGGM
Sbjct: 181 LPQKASGFLMKKELDYFSKALEKPERPFLAILGGAKVKDKIQLIKNMLDKVNFMIIGGGM 240
Query: 844 AFTFLK 861
A+TFLK
Sbjct: 241 AYTFLK 246
Score = 42.4 bits (98), Expect(2) = e-128
Identities = 18/28 (64%), Positives = 23/28 (82%)
Frame = +3
Query: 930 QAEKNGVKNTLPGDFVTAEQFDENAQIG 1013
+AEKNGVK P DFVT ++FDENA++G
Sbjct: 272 KAEKNGVKIVFPVDFVTGDKFDENAKVG 299
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 32,162,347
Number of extensions: 801775
Number of successful extensions: 2615
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 2615
Number of HSP's successfully gapped: 4
Length of query: 338
Length of database: 15,617,559
Length adjustment: 102
Effective length of query: 236
Effective length of database: 12,553,887
Effective search space: 2962717332
Effective search space used: 2962717332
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000604
(1015 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001093402.1| phosphoglycerate kinase 1 [Sus scrofa]. 484 e-137
Alignment gi|NP_998947.1| phosphoglycerate kinase 2 [Sus scrofa]. 439 e-128
Alignment gi|XP_003135255.2| PREDICTED: phosphoglycerate kinase 1-like, p... 284 6e-77
>ref|NP_001093402.1| phosphoglycerate kinase 1 [Sus scrofa].
Length = 417
Score = 484 bits (1247), Expect = e-137
Identities = 251/275 (91%), Positives = 258/275 (93%), Gaps = 2/275 (0%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDGIPMPDKYSLEPVAVELKSL GKDVLFLKDCVGPEVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGIPMPDKYSLEPVAVELKSLPGKDVLFLKDCVGPEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM
Sbjct: 181 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 240
Query: 844 AFTFLKCSTHGDWHFSFDERDPDR--QDLMSKLRR 942
AFTFLK + + S + + + +DLMSK +
Sbjct: 241 AFTFLKVLNNMEIGTSLFDEEGSKIVKDLMSKAEK 275
Score = 49.3 bits (116), Expect = 3e-06
Identities = 30/53 (56%), Positives = 38/53 (71%), Gaps = 2/53 (3%)
Frame = +3
Query: 861 VLNTWRLAL-LF**EGSRSSRSDV-QAEKNGVKNTLPGDFVTAEQFDENAQIG 1013
VLN + LF EGS+ + + +AEKNGVK TLP DFVTA++FDENA+IG
Sbjct: 247 VLNNMEIGTSLFDEEGSKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKIG 299
>ref|NP_998947.1| phosphoglycerate kinase 2 [Sus scrofa].
Length = 417
Score = 439 bits (1129), Expect(2) = e-128
Identities = 217/246 (88%), Positives = 235/246 (95%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLS KLTLDKLDVKGKRV+MRVDFNVPMK NQ+TNNQRIKA++PSI++CLDNGA+SVVL
Sbjct: 1 MSLSKKLTLDKLDVKGKRVIMRVDFNVPMKRNQVTNNQRIKASLPSIRYCLDNGARSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDG+ MPDKYSLEPVA ELKSLLGKDVLFLKDCVG E E+ACA+P AGSVILLE
Sbjct: 61 MSHLGRPDGVAMPDKYSLEPVAAELKSLLGKDVLFLKDCVGSEAEQACANPPAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 663
NLRFHVEEEGKG+D SG+K+KA+P K+EAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN
Sbjct: 121 NLRFHVEEEGKGQDPSGNKLKAEPDKVEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN 180
Query: 664 LPKKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGM 843
LP+KA GFLMKKEL+YFAKALE+PERPFLAILGGAKVADKIQLI NMLDKVNEMIIGGGM
Sbjct: 181 LPQKASGFLMKKELDYFAKALENPERPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGM 240
Query: 844 AFTFLK 861
AFTFLK
Sbjct: 241 AFTFLK 246
Score = 37.7 bits (86), Expect(2) = e-128
Identities = 24/53 (45%), Positives = 34/53 (64%), Gaps = 2/53 (3%)
Frame = +3
Query: 861 VLNTWRL-ALLF**EGSRSSRSDV-QAEKNGVKNTLPGDFVTAEQFDENAQIG 1013
VLN + A LF EG+ + + +AEKN V T P DFV A++F+ENA++G
Sbjct: 247 VLNNMEIGASLFDKEGATIVKEIMAKAEKNRVNITFPVDFVIADKFEENAKVG 299
>ref|XP_003135255.2| PREDICTED: phosphoglycerate kinase 1-like, partial [Sus scrofa].
Length = 149
Score = 284 bits (726), Expect = 6e-77
Identities = 143/145 (98%), Positives = 143/145 (98%)
Frame = +1
Query: 124 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 303
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL
Sbjct: 1 MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAIPSIKFCLDNGAKSVVL 60
Query: 304 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 483
MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE
Sbjct: 61 MSHLGRPDGIPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACADPAAGSVILLE 120
Query: 484 NLRFHVEEEGKGKDASGSKVKADPA 558
NLRFHVEEEGKGKDASGSKV DPA
Sbjct: 121 NLRFHVEEEGKGKDASGSKVGPDPA 145
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 23,809,124
Number of extensions: 602873
Number of successful extensions: 1863
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 1860
Number of HSP's successfully gapped: 5
Length of query: 338
Length of database: 11,343,932
Length adjustment: 100
Effective length of query: 238
Effective length of database: 8,854,232
Effective search space: 2107307216
Effective search space used: 2107307216
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-000604
(1015 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_Chr07 543 e-152
Sscrofa_ChrX 363 1e-97
>Sscrofa_Chr07
|| Length = 134764511
Score = 543 bits (274), Expect = e-152
Identities = 648/770 (84%), Gaps = 2/770 (0%)
Strand = Plus / Minus
Query: 115 attgccaagatgtcgctttctaacaagctgactctggacaagctggacgtgaagggaaag 174
|||| ||| ||||| |||||||| ||| | ||| ||||||| ||||||||||| || ||
Sbjct: 50232950 attgtcaaaatgtctctttctaagaagttaactttggacaaactggacgtgaaagggaaa 50232891
Query: 175 agggtcgtcatgagagtggacttcaatgttcctatgaagaacaaccagataacgaacaac 234
| ||| |||||||||| |||||||||||||| ||||||| ||||| | || ||||||
Sbjct: 50232890 cgagtcatcatgagagtagacttcaatgttcccatgaagaggaaccaagttacaaacaac 50232831
Query: 235 cagaggatcaaggctgccatcccaagcatcaaattctgcctagacaacggagccaagtca 294
|| || ||||||||| || |||||||||||| || ||||||||| |||||||||| ||||
Sbjct: 50232830 caaagaatcaaggcttccctcccaagcatcagatactgcctagataacggagccaggtca 50232771
Query: 295 gttgttcttatgagccacctaggccggcctgatggtatccctatgcctgacaagtactcc 354
|| ||||| ||||| || |||| | ||||||||| | | |||||||| || ||||||
Sbjct: 50232770 gtagttctcatgagtcatttaggtagacctgatggtgttgcgatgcctgataaatactcc 50232711
Query: 355 ttggagccagttgctgtagaactcaaatctctgctgggcaaggatgttttgttcttgaag 414
|| ||||| || |||| ||||||||||| ||||||| || || ||| ||||| | ||
Sbjct: 50232710 ttagagccggtggctgctgaactcaaatccttgctggggaaagacgttctgttcctaaaa 50232651
Query: 415 gactgcgtgggcccagaagtggagaaagcttgtgctgacccagctgctggttctgtcatc 474
||||| |||||| |||||| ||| |||||||||| ||||| |||| ||||| ||||||
Sbjct: 50232650 gactgtgtgggctcagaagcagagcaagcttgtgccaacccacctgcgggttcagtcatc 50232591
Query: 475 ctgttggagaaccttcgctttcatgtggaggaagaagggaagggaaaagatgcttctggg 534
|| ||||||||||||||||||||||||| || ||||||||||| ||||| ||||||||
Sbjct: 50232590 ctactggagaaccttcgctttcatgtggaagaggaagggaagggccaagatccttctggg 50232531
Query: 535 agcaaggttaaagctgatccagccaaaatagaagccttccgagcttcactttccaagcta 594
| ||| | |||||||| |||| ||| ||||||||||||| || || || ||||||||
Sbjct: 50232530 aataagctgaaagctgagccagataaagtagaagccttccgcgcatcgctctccaagctg 50232471
Query: 595 ggggatgtctatgtcaatgatgcttttggtactgctcaccgagctcacagctccatggtg 654
|||||||| |||||||||||||||||||| || |||||||| |||||||| |||||||||
Sbjct: 50232470 ggggatgtgtatgtcaatgatgcttttggcacggctcaccgggctcacagttccatggtg 50232411
Query: 655 ggagtaaatctgccaaagaaggctggaggttttttgatgaagaaggagctgaactacttt 714
||||| |||||||| ||||||| || || ||||||| ||||||||| | ||||||
Sbjct: 50232410 ggagtgaatctgccgcagaaggcatctggattcctgatgaaaaaggagctggattacttt 50232351
Query: 715 gccaaggccttggagagcccagagcgacccttcctggccatcctgggcggagctaaagtt 774
||||| |||||||| | ||||||| ||||||| ||||| ||||| || |||||||||||
Sbjct: 50232350 gccaaagccttggaaaacccagagagaccctttctggctatccttggtggagctaaagtg 50232291
Query: 775 gcagacaagatccagctaatcaataatatgctggacaaagtcaatgagatgattattggt 834
|||||||||||||| || ||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 50232290 gcagacaagatccaactcatcaaaaatatgctggacaaagtcaatgagatgattattggt 50232231
Query: 835 ggtggaatggcctttaccttccttaa-gtgctcaac-acatggagattgg 882
|| ||||||||||||||||||||||| || |||||| |||||||||||||
Sbjct: 50232230 ggcggaatggcctttaccttccttaaggtactcaacaacatggagattgg 50232181
>Sscrofa_ChrX
|| Length = 144288218
Score = 363 bits (183), Expect = 1e-97
Identities = 186/187 (99%)
Strand = Plus / Plus
Query: 2 gggaggagggacggcggggccagcggtgtgctcggcagtttccacgcgttcggattgcga 61
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71106630 gggaggagggacggcggggccagcggtgtgctcggcagtttccacgcgttcggattgcga 71106689
Query: 62 attggttgtcgtttctgcggcagtctcatctcccggtctctcgccgccactgaattgcca 121
||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct: 71106690 attggttgtcgtttctgcggcagtctcatctccgggtctctcgccgccactgaattgcca 71106749
Query: 122 agatgtcgctttctaacaagctgactctggacaagctggacgtgaagggaaagagggtcg 181
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71106750 agatgtcgctttctaacaagctgactctggacaagctggacgtgaagggaaagagggtcg 71106809
Query: 182 tcatgag 188
|||||||
Sbjct: 71106810 tcatgag 71106816
Score = 315 bits (159), Expect = 3e-83
Identities = 159/159 (100%)
Strand = Plus / Plus
Query: 238 aggatcaaggctgccatcccaagcatcaaattctgcctagacaacggagccaagtcagtt 297
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71117503 aggatcaaggctgccatcccaagcatcaaattctgcctagacaacggagccaagtcagtt 71117562
Query: 298 gttcttatgagccacctaggccggcctgatggtatccctatgcctgacaagtactccttg 357
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71117563 gttcttatgagccacctaggccggcctgatggtatccctatgcctgacaagtactccttg 71117622
Query: 358 gagccagttgctgtagaactcaaatctctgctgggcaag 396
|||||||||||||||||||||||||||||||||||||||
Sbjct: 71117623 gagccagttgctgtagaactcaaatctctgctgggcaag 71117661
Score = 315 bits (159), Expect = 3e-83
Identities = 159/159 (100%)
Strand = Plus / Minus
Query: 238 aggatcaaggctgccatcccaagcatcaaattctgcctagacaacggagccaagtcagtt 297
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71351768 aggatcaaggctgccatcccaagcatcaaattctgcctagacaacggagccaagtcagtt 71351709
Query: 298 gttcttatgagccacctaggccggcctgatggtatccctatgcctgacaagtactccttg 357
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71351708 gttcttatgagccacctaggccggcctgatggtatccctatgcctgacaagtactccttg 71351649
Query: 358 gagccagttgctgtagaactcaaatctctgctgggcaag 396
|||||||||||||||||||||||||||||||||||||||
Sbjct: 71351648 gagccagttgctgtagaactcaaatctctgctgggcaag 71351610
Score = 291 bits (147), Expect = 4e-76
Identities = 147/147 (100%)
Strand = Plus / Plus
Query: 396 ggatgttttgttcttgaaggactgcgtgggcccagaagtggagaaagcttgtgctgaccc 455
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71117777 ggatgttttgttcttgaaggactgcgtgggcccagaagtggagaaagcttgtgctgaccc 71117836
Query: 456 agctgctggttctgtcatcctgttggagaaccttcgctttcatgtggaggaagaagggaa 515
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71117837 agctgctggttctgtcatcctgttggagaaccttcgctttcatgtggaggaagaagggaa 71117896
Query: 516 gggaaaagatgcttctgggagcaaggt 542
|||||||||||||||||||||||||||
Sbjct: 71117897 gggaaaagatgcttctgggagcaaggt 71117923
Score = 291 bits (147), Expect = 4e-76
Identities = 147/147 (100%)
Strand = Plus / Minus
Query: 396 ggatgttttgttcttgaaggactgcgtgggcccagaagtggagaaagcttgtgctgaccc 455
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71351494 ggatgttttgttcttgaaggactgcgtgggcccagaagtggagaaagcttgtgctgaccc 71351435
Query: 456 agctgctggttctgtcatcctgttggagaaccttcgctttcatgtggaggaagaagggaa 515
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71351434 agctgctggttctgtcatcctgttggagaaccttcgctttcatgtggaggaagaagggaa 71351375
Query: 516 gggaaaagatgcttctgggagcaaggt 542
|||||||||||||||||||||||||||
Sbjct: 71351374 gggaaaagatgcttctgggagcaaggt 71351348
Score = 242 bits (122), Expect = 3e-61
Identities = 122/122 (100%)
Strand = Plus / Minus
Query: 643 agctccatggtgggagtaaatctgccaaagaaggctggaggttttttgatgaagaaggag 702
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71346633 agctccatggtgggagtaaatctgccaaagaaggctggaggttttttgatgaagaaggag 71346574
Query: 703 ctgaactactttgccaaggccttggagagcccagagcgacccttcctggccatcctgggc 762
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71346573 ctgaactactttgccaaggccttggagagcccagagcgacccttcctggccatcctgggc 71346514
Query: 763 gg 764
||
Sbjct: 71346513 gg 71346512
Score = 210 bits (106), Expect = 1e-51
Identities = 106/106 (100%)
Strand = Plus / Minus
Query: 539 aggttaaagctgatccagccaaaatagaagccttccgagcttcactttccaagctagggg 598
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71347630 aggttaaagctgatccagccaaaatagaagccttccgagcttcactttccaagctagggg 71347571
Query: 599 atgtctatgtcaatgatgcttttggtactgctcaccgagctcacag 644
||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71347570 atgtctatgtcaatgatgcttttggtactgctcaccgagctcacag 71347525
Score = 198 bits (100), Expect = 4e-48
Identities = 107/108 (99%), Gaps = 1/108 (0%)
Strand = Plus / Minus
Query: 764 gagctaaagttgcagacaagatccagctaatcaataatatgctggacaaagtcaatgaga 823
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71343311 gagctaaagttgcagacaagatccagctaatcaataatatgctggacaaagtcaatgaga 71343252
Query: 824 tgattattggtggtggaatggcctttaccttccttaa-gtgctcaaca 870
||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 71343251 tgattattggtggtggaatggcctttaccttccttaaggtgctcaaca 71343204
Score = 168 bits (85), Expect = 4e-39
Identities = 135/145 (93%), Gaps = 5/145 (3%)
Strand = Plus / Minus
Query: 876 agattggcacttctctttt-gatga-gagggatcca-gatcgtcaa-gatctgatgtcca 931
||||||||||||||||||| ||||| |||||||||| ||||||||| |||||||||||||
Sbjct: 71342591 agattggcacttctctttttgatgaagagggatccaagatcgtcaaagatctgatgtcca 71342532
Query: 932 a-gctgagaagaatggtgtgaagaatactttgcctggtgactttgtcactgctgaacagt 990
| |||||||||||||||||||||| ||||||||||| |||||||||||||||||| |||
Sbjct: 71342531 aagctgagaagaatggtgtgaagattactttgcctgttgactttgtcactgctgacaagt 71342472
Query: 991 ttgatgagaatgcccagattggcca 1015
|||||||||||||| ||||||||||
Sbjct: 71342471 ttgatgagaatgccaagattggcca 71342447
Score = 107 bits (54), Expect = 1e-20
Identities = 54/54 (100%)
Strand = Plus / Plus
Query: 187 agagtggacttcaatgttcctatgaagaacaaccagataacgaacaaccagagg 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 71112990 agagtggacttcaatgttcctatgaagaacaaccagataacgaacaaccagagg 71113043
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 29,273,302
Number of extensions: 211
Number of successful extensions: 211
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 209
Number of HSP's successfully gapped: 12
Length of query: 1015
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 994
Effective length of database: 2,808,413,156
Effective search space: 2791562677064
Effective search space used: 2791562677064
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 29 (58.0 bits)