Animal-Genome cDNA 20110601C-008177

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008177
         (1006 letters)

Database: RefSeq49_BP.fasta 
           33,088 sequences; 17,681,374 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|NP_001030457.1| vitamin D-binding protein precursor [Bos tau...   573   e-165
Alignment   gi|NP_851335.1| serum albumin precursor [Bos taurus].                122   6e-28
Alignment   gi|NP_001029434.1| alpha-fetoprotein precursor [Bos taurus].         106   4e-23
Alignment   gi|NP_001179104.1| afamin [Bos taurus].                              100   2e-21
Alignment   gi|XP_002688396.1| PREDICTED: afamin [Bos taurus].                   100   2e-21

>ref|NP_001030457.1| vitamin D-binding protein precursor [Bos taurus].
          Length = 474

 Score =  573 bits (1478), Expect(2) = e-165
 Identities = 275/305 (90%), Positives = 291/305 (95%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 241
           MKRIL+FLLAVAFVHALERGRDYEKDKVCK+LASLG++DFTSLSMVLYSRKFPSGTFEQI
Sbjct: 1   MKRILVFLLAVAFVHALERGRDYEKDKVCKDLASLGREDFTSLSMVLYSRKFPSGTFEQI 60

Query: 242 SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 421
           SHLVNEVVSLT  CCAEGADPDCYDNRTSALSDKSCES+SPFPVHPGT ECCT EGLEKK
Sbjct: 61  SHLVNEVVSLTVTCCAEGADPDCYDNRTSALSDKSCESNSPFPVHPGTPECCTHEGLEKK 120

Query: 422 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 601
           LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPK FA++FMYEYSINYGQAPLTLLV YTK
Sbjct: 121 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKDFADRFMYEYSINYGQAPLTLLVGYTK 180

Query: 602 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           SYLSMVGSCCTSP+PTVCFLKERLQLKH SLLTIM+NR+CSQYAAYGK+KSRLSHLIK A
Sbjct: 181 SYLSMVGSCCTSPNPTVCFLKERLQLKHFSLLTIMTNRICSQYAAYGKEKSRLSHLIKFA 240

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QKVPTA+LEDVLPLAED+ TILSKCCDS SEDC+ KELPEY VK+CDNLS+KNSKF DCC
Sbjct: 241 QKVPTAHLEDVLPLAEDITTILSKCCDSVSEDCI-KELPEYAVKLCDNLSTKNSKFKDCC 299

Query: 962 QEKHP 976
           QEK P
Sbjct: 300 QEKTP 304



 Score = 29.3 bits (64), Expect(2) = e-165
 Identities = 10/13 (76%), Positives = 12/13 (92%)
 Frame = +1

Query: 967  KTPMDIFICTYFM 1005
            KTPM+IF+C YFM
Sbjct: 302  KTPMEIFVCAYFM 314



 Score = 71.2 bits (173), Expect = 1e-12
 Identities = 60/249 (24%), Positives = 104/249 (41%), Gaps = 8/249 (3%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           +++C + A+ GK+      ++ +++K P+   E +  L  ++ ++   CC +    DC  
Sbjct: 217 NRICSQYAAYGKEKSRLSHLIKFAQKVPTAHLEDVLPLAEDITTILSKCC-DSVSEDCIK 275

Query: 317 NRTS---ALSDKSCESDSPFPVHPGTAECCT-KEGLEKKLCMAALKHQPQ-EFPTYVEPT 481
                   L D     +S F       +CC  K  +E  +C   +   P  E P    P 
Sbjct: 276 ELPEYAVKLCDNLSTKNSKFK------DCCQEKTPMEIFVCAYFMPASPNPELPDVKLPM 329

Query: 482 NDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFL 661
           N ++C+   +      +Q+++E S    Q P   L    +S L  +  CC S S T C  
Sbjct: 330 NKDVCD---EGNTKVLDQYIFELS-RKTQIPEVFLTKILESTLKSLDECCHSESSTACLN 385

Query: 662 KERLQL-KHLSLLTIMSNRLCSQYA--AYGKDKSRLSHLIKLAQKVPTANLEDVLPLAED 832
            +  QL + LS        LC+ Y+   + + K +L+   +L  K P A   D+  L   
Sbjct: 386 AKGPQLTRELSSFIQKGQELCADYSENTFTEYKKKLAE--RLRGKFPDATETDLQELVAK 443

Query: 833 VATILSKCC 859
            +   SKCC
Sbjct: 444 RSDFASKCC 452


>ref|NP_851335.1| serum albumin precursor [Bos taurus].
          Length = 607

 Score =  122 bits (305), Expect = 6e-28
 Identities = 74/306 (24%), Positives = 136/306 (44%), Gaps = 4/306 (1%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERG---RDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTF 232
           MK +    L + F  A  RG   RD  K ++      LG++ F  L ++ +S+      F
Sbjct: 1   MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPF 60

Query: 233 EQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGL 412
           ++   LVNE+    + C A+ +   C  +  +   D+ C+  S    +   A+CC K+  
Sbjct: 61  DEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEP 120

Query: 413 EKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVS 592
           E+  C  + K    + P  ++P  + +C+ F+ D K F  +++YE +  +       L+ 
Sbjct: 121 ERNECFLSHKDDSPDLPK-LKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLY 179

Query: 593 YTKSYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRL-CSQYAAYGKDKSRLSHL 769
           Y   Y  +   CC +     C L +   ++   L +    RL C+    +G+   +   +
Sbjct: 180 YANKYNGVFQECCQAEDKGACLLPKIETMREKVLTSSARQRLRCASIQKFGERALKAWSV 239

Query: 770 IKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKF 949
            +L+QK P A   +V  L  D+  +  +CC     +C A +  +    ICDN  + +SK 
Sbjct: 240 ARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLEC-ADDRADLAKYICDNQDTISSKL 298

Query: 950 TDCCQE 967
            +CC +
Sbjct: 299 KECCDK 304



 Score = 95.9 bits (237), Expect = 5e-20
 Identities = 64/284 (22%), Positives = 128/284 (45%), Gaps = 10/284 (3%)
 Frame = +2

Query: 146  CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
            C  +   G+    + S+   S+KFP   F +++ LV ++  + + CC  G   +C D+R 
Sbjct: 223  CASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECC-HGDLLECADDRA 281

Query: 326  SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQ--PQEFPTYVEP--TNDEI 493
              L+   C++     +     ECC K  LEK  C+A ++    P+  P        + ++
Sbjct: 282  D-LAKYICDNQDT--ISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDV 338

Query: 494  CEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERL 673
            C+ +++    F   F+YEYS  + +  +++L+   K Y + +  CC    P  C+     
Sbjct: 339  CKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFD 398

Query: 674  QLKHL-SLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILS 850
            +LKHL      +  + C Q+   G+   + + +++  +KVP  +   ++ ++  +  + +
Sbjct: 399  KLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGT 458

Query: 851  KCC---DSASEDCMAKELPEYTVKIC--DNLSSKNSKFTDCCQE 967
            +CC   +S    C    L     ++C     +  + K T CC E
Sbjct: 459  RCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTE 502



 Score = 51.6 bits (122), Expect = 1e-06
 Identities = 43/184 (23%), Positives = 74/184 (40%), Gaps = 10/184 (5%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCA--EGADPDCYDN 319
           C +   LG+  F +  +V Y+RK P  +   +  +   +  +   CC   E     C ++
Sbjct: 415 CDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTED 474

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICE 499
             S + ++ C      PV     +CCT+  + ++ C +AL   P E  TYV    DE   
Sbjct: 475 YLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALT--PDE--TYVPKAFDEKLF 530

Query: 500 AFRKD-------PKGFANQF-MYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVC 655
            F  D        K    Q  + E   +  +A    L +  +++++ V  CC +     C
Sbjct: 531 TFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEAC 590

Query: 656 FLKE 667
           F  E
Sbjct: 591 FAVE 594


>ref|NP_001029434.1| alpha-fetoprotein precursor [Bos taurus].
          Length = 610

 Score =  106 bits (264), Expect = 4e-23
 Identities = 66/301 (21%), Positives = 139/301 (46%), Gaps = 4/301 (1%)
 Frame = +2

Query: 74  LIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLV 253
           L+FLL  +    + +   Y  D +          +   L+ + +++     T+E++S +V
Sbjct: 9   LLFLLNFSDSRTMHKNA-YGIDSILDSSPCSSGTNLVGLATIFFAQSVQGATYEEVSQMV 67

Query: 254 NEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLC-M 430
            +V+++ E          C +N+ SA  ++ C  +   P   G ++CC++ G E+  C +
Sbjct: 68  KDVLTIIEKPTGSKQPAGCLENQVSAFLEEICR-EKEIPEKYGLSDCCSRTGEERHDCFL 126

Query: 431 AALKHQPQEFPTY--VEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKS 604
           A  K  P   P +  +EP     C++++++ + F N+++YE +  +       ++S    
Sbjct: 127 AHKKAAPASIPPFPVLEPVTS--CKSYKENRELFINRYIYEIARRHPVLYAPTILSVANQ 184

Query: 605 YLSMVGSCCTSPSPTVCFLKERLQL-KHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           Y  ++  CC + + T CF  +   + K L   ++++  +C+    +G    R   + K++
Sbjct: 185 YNKIIPHCCKAENATECFETKVTSITKELRESSLLNQHICAVMGKFGPRTFRAITVTKVS 244

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QK P AN  ++  L  DVA I  +CC     +C+ ++       IC      + +  +CC
Sbjct: 245 QKFPKANFTEIQKLVMDVAHIHEECCKGNVLECL-QDGERVMSYICSQQDILSRQIAECC 303

Query: 962 Q 964
           +
Sbjct: 304 K 304



 Score = 67.4 bits (163), Expect = 2e-11
 Identities = 53/255 (20%), Positives = 108/255 (42%), Gaps = 7/255 (2%)
 Frame = +2

Query: 143 VCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNR 322
           +C  +   G   F ++++   S+KFP   F +I  LV +V  + E CC +G   +C  + 
Sbjct: 223 ICAVMGKFGPRTFRAITVTKVSQKFPKANFTEIQKLVMDVAHIHEECC-KGNVLECLQDG 281

Query: 323 TSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPT------N 484
              +S    + D    +    AECC K     +L    +  +  + P  + P       +
Sbjct: 282 ERVMSYICSQQDI---LSRQIAECC-KLPTTLELGHCIIHAENDDKPEGLSPNVNRFLGD 337

Query: 485 DEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLK 664
            +  +   +D      +F YEYS  + +  + +++   K Y  ++  C  S +P+ C  K
Sbjct: 338 RDFNQLSSRDKDLSMARFTYEYSRRHTKLAVPIILRVAKGYQELLEKCSQSENPSECQDK 397

Query: 665 ERLQL-KHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVAT 841
              +L K++     ++ R C  +   G+   + + L+   +K P     +++ L   +A 
Sbjct: 398 GEEELEKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSPELMALTRKMAN 457

Query: 842 ILSKCCDSASEDCMA 886
             + CC  + +  +A
Sbjct: 458 AGAICCHLSEDKQLA 472



 Score = 50.8 bits (120), Expect = 2e-06
 Identities = 38/184 (20%), Positives = 68/184 (36%), Gaps = 10/184 (5%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPD--CYDN 319
           C     LG+    +  +V Y++K P  T  ++  L  ++ +    CC    D    C + 
Sbjct: 417 CGLFQKLGEYYLQNAFLVAYTKKAPQLTSPELMALTRKMANAGAICCHLSEDKQLACGEG 476

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPT------ 481
               +    C      P++PG  +CCT     ++ C ++L        TYV P       
Sbjct: 477 VADLIIGHLCIRHEENPINPGVDQCCTSSYSNRRPCFSSLVVD----ETYVPPPFSDDKF 532

Query: 482 --NDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVC 655
             + ++C+      +    QF+        Q     L +    +  ++  CC S    VC
Sbjct: 533 IFHKDLCQVQGVPLQTMKQQFLINLVKQKPQITEEQLETVVADFSGLLEKCCQSQEQEVC 592

Query: 656 FLKE 667
           F +E
Sbjct: 593 FTEE 596


>ref|NP_001179104.1| afamin [Bos taurus].
          Length = 604

 Score =  100 bits (250), Expect = 2e-21
 Identities = 71/302 (23%), Positives = 137/302 (45%), Gaps = 10/302 (3%)
 Frame = +2

Query: 89   AVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVS 268
            A  F++ L+    Y+K+  C+ L   G+    S+++ + S+KFP   F+Q++ L+ +V S
Sbjct: 206  AEPFIYYLKALSSYQKN-ACRALMKFGRQILQSINIAILSQKFPKIGFKQLTSLLEDVSS 264

Query: 269  LTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCM--AALK 442
              + CC EG    C   R+  +S    + DS   +     +CC K+  E+  C+  +   
Sbjct: 265  KYDGCC-EGDVVQCIRGRSKVMSHICSKQDS---ISSKIKDCCEKKIPERGECIIYSNKD 320

Query: 443  HQPQEFPT----YVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYL 610
             +P +       ++E  +D +CE    D   F  +F+YEYS  + +     L+   K Y 
Sbjct: 321  DRPNDLSLREAKFIE--SDNVCEKRDADQANFMAEFLYEYSRRHPELSTPELLRIAKVYK 378

Query: 611  SMVGSCCTSPSPTVCFLKERLQLKHLSLLTI-MSNRLCSQYAAYGKDKSRLSHLIKLAQK 787
             ++  CC   +P  C+     +    +  ++ +  R C  +   GKD  +  +LI L + 
Sbjct: 379  DLLKECCNMENPPECYRHAENRFNETTEKSLKIVQRECEHFQNLGKDDLKYHYLINLTKL 438

Query: 788  VPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTV-KICDNLSSK--NSKFTDC 958
             P  + E++  L +++   L+ CC  + E      L +  + ++C    ++  N     C
Sbjct: 439  APQLSTEELTFLGKEMVMALTTCCTLSEEFACVDNLVDLVLGELCGINENRNINPAVDHC 498

Query: 959  CQ 964
            C+
Sbjct: 499  CK 500



 Score = 91.3 bits (225), Expect = 1e-18
 Identities = 61/273 (22%), Positives = 115/273 (42%), Gaps = 5/273 (1%)
 Frame = +2

Query: 173 DDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCE 352
           D+   ++++ +++     +FE++  LV  +    + C A+   P+C       L +  C 
Sbjct: 41  DNIGYITIIAFAQYIQEASFEEVEMLVKAMTEYRDKCLADRTLPECSKLANEVLLENICA 100

Query: 353 SDSPFPVHPGTAECCTKEGLEKKLCMAALKHQP----QEFPTYVEPTNDEICEAFRKDPK 520
            +   P     + CC K   E++LC    K          PT ++P  +E C+ ++ + +
Sbjct: 101 MEG-LPQKYNFSHCCHKVDFERRLCFFHNKKADIGLLPPLPT-LDP--EEKCQTYKNNRE 156

Query: 521 GFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LKERLQLKHLSLL 697
            F N ++YE S          L++    +  M  +CC       CF  K    + +L  L
Sbjct: 157 SFLNNYVYEVSRRNPFVFAPTLLTVAARFEEMTKTCCEEQEKANCFQTKAEPFIYYLKAL 216

Query: 698 TIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASED 877
           +      C     +G+   +  ++  L+QK P    + +  L EDV++    CC+     
Sbjct: 217 SSYQKNACRALMKFGRQILQSINIAILSQKFPKIGFKQLTSLLEDVSSKYDGCCEGDVVQ 276

Query: 878 CMAKELPEYTVKICDNLSSKNSKFTDCCQEKHP 976
           C+ +   +    IC    S +SK  DCC++K P
Sbjct: 277 CI-RGRSKVMSHICSKQDSISSKIKDCCEKKIP 308



 Score = 59.3 bits (142), Expect = 5e-09
 Identities = 39/186 (20%), Positives = 77/186 (41%), Gaps = 8/186 (4%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
           C+   +LGKDD     ++  ++  P  + E+++ L  E+V     CC    +  C DN  
Sbjct: 416 CEHFQNLGKDDLKYHYLINLTKLAPQLSTEELTFLGKEMVMALTTCCTLSEEFACVDNLV 475

Query: 326 SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTND------ 487
             +  + C  +    ++P    CC      ++ C  +L+       TYV P+        
Sbjct: 476 DLVLGELCGINENRNINPAVDHCCKTNFAFRRSCFESLEAD----KTYVPPSTSQGLFTF 531

Query: 488 --EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFL 661
             ++C+A  ++ +   ++F+        +     L S    + ++V  CC +  P  CF 
Sbjct: 532 HADLCQAHNEELQRKKDRFLVNLVKLKPELAGEELWSLLADFTNVVEKCCKAQEPEACFK 591

Query: 662 KERLQL 679
           +E  +L
Sbjct: 592 EESPKL 597


>ref|XP_002688396.1| PREDICTED: afamin [Bos taurus].
          Length = 604

 Score =  100 bits (250), Expect = 2e-21
 Identities = 71/302 (23%), Positives = 137/302 (45%), Gaps = 10/302 (3%)
 Frame = +2

Query: 89   AVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVS 268
            A  F++ L+    Y+K+  C+ L   G+    S+++ + S+KFP   F+Q++ L+ +V S
Sbjct: 206  AEPFIYYLKALSSYQKN-ACRALMKFGRQILQSINIAILSQKFPKIGFKQLTSLLEDVSS 264

Query: 269  LTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCM--AALK 442
              + CC EG    C   R+  +S    + DS   +     +CC K+  E+  C+  +   
Sbjct: 265  KYDGCC-EGDVVQCIRGRSKVMSHICSKQDS---ISSKIKDCCEKKIPERGECIIYSNKD 320

Query: 443  HQPQEFPT----YVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYL 610
             +P +       ++E  +D +CE    D   F  +F+YEYS  + +     L+   K Y 
Sbjct: 321  DRPNDLSLREAKFIE--SDNVCEKRDADQANFMAEFLYEYSRRHPELSTPELLRIAKVYK 378

Query: 611  SMVGSCCTSPSPTVCFLKERLQLKHLSLLTI-MSNRLCSQYAAYGKDKSRLSHLIKLAQK 787
             ++  CC   +P  C+     +    +  ++ +  R C  +   GKD  +  +LI L + 
Sbjct: 379  DLLKECCNMENPPECYRHAENRFNETTEKSLKIVQRECEHFQNLGKDDLKYHYLINLTKL 438

Query: 788  VPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTV-KICDNLSSK--NSKFTDC 958
             P  + E++  L +++   L+ CC  + E      L +  + ++C    ++  N     C
Sbjct: 439  APQLSTEELTFLGKEMVMALTTCCTLSEEFACVDNLVDLVLGELCGINENRNINPAVDHC 498

Query: 959  CQ 964
            C+
Sbjct: 499  CK 500



 Score = 91.3 bits (225), Expect = 1e-18
 Identities = 61/273 (22%), Positives = 115/273 (42%), Gaps = 5/273 (1%)
 Frame = +2

Query: 173 DDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCE 352
           D+   ++++ +++     +FE++  LV  +    + C A+   P+C       L +  C 
Sbjct: 41  DNIGYITIIAFAQYIQEASFEEVEMLVKAMTEYRDKCLADRTLPECSKLANEVLLENICA 100

Query: 353 SDSPFPVHPGTAECCTKEGLEKKLCMAALKHQP----QEFPTYVEPTNDEICEAFRKDPK 520
            +   P     + CC K   E++LC    K          PT ++P  +E C+ ++ + +
Sbjct: 101 MEG-LPQKYNFSHCCHKVDFERRLCFFHNKKADIGLLPPLPT-LDP--EEKCQTYKNNRE 156

Query: 521 GFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LKERLQLKHLSLL 697
            F N ++YE S          L++    +  M  +CC       CF  K    + +L  L
Sbjct: 157 SFLNNYVYEVSRRNPFVFAPTLLTVAARFEEMTKTCCEEQEKANCFQTKAEPFIYYLKAL 216

Query: 698 TIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASED 877
           +      C     +G+   +  ++  L+QK P    + +  L EDV++    CC+     
Sbjct: 217 SSYQKNACRALMKFGRQILQSINIAILSQKFPKIGFKQLTSLLEDVSSKYDGCCEGDVVQ 276

Query: 878 CMAKELPEYTVKICDNLSSKNSKFTDCCQEKHP 976
           C+ +   +    IC    S +SK  DCC++K P
Sbjct: 277 CI-RGRSKVMSHICSKQDSISSKIKDCCEKKIP 308



 Score = 59.3 bits (142), Expect = 5e-09
 Identities = 39/186 (20%), Positives = 77/186 (41%), Gaps = 8/186 (4%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
           C+   +LGKDD     ++  ++  P  + E+++ L  E+V     CC    +  C DN  
Sbjct: 416 CEHFQNLGKDDLKYHYLINLTKLAPQLSTEELTFLGKEMVMALTTCCTLSEEFACVDNLV 475

Query: 326 SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTND------ 487
             +  + C  +    ++P    CC      ++ C  +L+       TYV P+        
Sbjct: 476 DLVLGELCGINENRNINPAVDHCCKTNFAFRRSCFESLEAD----KTYVPPSTSQGLFTF 531

Query: 488 --EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFL 661
             ++C+A  ++ +   ++F+        +     L S    + ++V  CC +  P  CF 
Sbjct: 532 HADLCQAHNEELQRKKDRFLVNLVKLKPELAGEELWSLLADFTNVVEKCCKAQEPEACFK 591

Query: 662 KERLQL 679
           +E  +L
Sbjct: 592 EESPKL 597


  Database: RefSeq49_BP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 17,681,374
  Number of sequences in database:  33,088
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 37,968,189
Number of extensions: 977956
Number of successful extensions: 2925
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 2890
Number of HSP's successfully gapped: 15
Length of query: 335
Length of database: 17,681,374
Length adjustment: 103
Effective length of query: 232
Effective length of database: 14,273,310
Effective search space: 3311407920
Effective search space used: 3311407920
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008177
         (1006 letters)

Database: RefSeq49_CP.fasta 
           33,336 sequences; 18,874,504 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|XP_863089.1| PREDICTED: similar to Vitamin D-binding protein...   572   e-165
Alignment   gi|XP_532404.2| PREDICTED: similar to Vitamin D-binding protein...   572   e-165
Alignment   gi|NP_001003026.1| serum albumin precursor [Canis lupus familia...   119   5e-27
Alignment   gi|XP_855557.1| PREDICTED: serum albumin [Canis familiaris].         119   5e-27
Alignment   gi|XP_532408.2| PREDICTED: similar to Afamin precursor (Alpha-a...    97   2e-20
Alignment   gi|NP_001003027.1| alpha-fetoprotein precursor [Canis lupus fam...    95   9e-20
Alignment   gi|XP_854726.1| PREDICTED: similar to Afamin precursor (Alpha-a...    75   8e-14

>ref|XP_863089.1| PREDICTED: similar to Vitamin D-binding protein precursor (DBP)
           (Group-specific component) (Gc-globulin) (VDB) isoform 2
           [Canis familiaris].
          Length = 474

 Score =  572 bits (1473), Expect(2) = e-165
 Identities = 272/305 (89%), Positives = 289/305 (94%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 241
           MKRIL+FL AVAFVHALERGRDYEK+KVCKELA+LGKDDFTSLSMVLYSRKFPS TFEQI
Sbjct: 1   MKRILVFLFAVAFVHALERGRDYEKEKVCKELANLGKDDFTSLSMVLYSRKFPSSTFEQI 60

Query: 242 SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 421
           SHLVNEVVSLTE CCAEGADPDCYD+RTSALS +SCE DSPFPVHPGTAECCT+EGLE+K
Sbjct: 61  SHLVNEVVSLTETCCAEGADPDCYDHRTSALSARSCEKDSPFPVHPGTAECCTREGLERK 120

Query: 422 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 601
           LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPK FA +FMYEYSINYGQAPL+LLV YTK
Sbjct: 121 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKDFAEKFMYEYSINYGQAPLSLLVGYTK 180

Query: 602 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           SYLSMVGSCCTS SPT CFLKERLQ+KHLSLLT MSNR+CSQYAAYGK+KSRLSHLIKLA
Sbjct: 181 SYLSMVGSCCTSSSPTACFLKERLQMKHLSLLTTMSNRICSQYAAYGKEKSRLSHLIKLA 240

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QKVPTA LEDVLPLAE++ T+LSKCC+S SEDCMAKELPEYTVKICDNLS+KNSKF DCC
Sbjct: 241 QKVPTAELEDVLPLAEEINTVLSKCCESTSEDCMAKELPEYTVKICDNLSTKNSKFKDCC 300

Query: 962 QEKHP 976
           QEK P
Sbjct: 301 QEKTP 305



 Score = 30.8 bits (68), Expect(2) = e-165
 Identities = 11/13 (84%), Positives = 12/13 (92%)
 Frame = +1

Query: 967  KTPMDIFICTYFM 1005
            KTPMDIF+C YFM
Sbjct: 303  KTPMDIFVCAYFM 315



 Score = 68.2 bits (165), Expect = 1e-11
 Identities = 58/246 (23%), Positives = 104/246 (42%), Gaps = 5/246 (2%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           +++C + A+ GK+      ++  ++K P+   E +  L  E+ ++   CC E    DC  
Sbjct: 217 NRICSQYAAYGKEKSRLSHLIKLAQKVPTAELEDVLPLAEEINTVLSKCC-ESTSEDCMA 275

Query: 317 NRTSALSDKSCESDSPFPVHPGTAECCT-KEGLEKKLCMAALKHQPQ-EFPTYVEPTNDE 490
                 + K C++ S    +    +CC  K  ++  +C   +   P  E P    P N +
Sbjct: 276 KELPEYTVKICDNLST--KNSKFKDCCQEKTPMDIFVCAYFMPAAPTPELPAIELPANAD 333

Query: 491 ICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKER 670
           +C+   K      +Q+ +E S      P   L    +  L  +  CC S   T C   + 
Sbjct: 334 VCD---KGNTKAIDQYTFELS-RRTHLPEVFLSKILEPTLKSLAECCDSEDSTGCMNAQG 389

Query: 671 LQL-KHLSLLTIMSNRLCSQYA--AYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVAT 841
            QL K LS       +LC+ Y+   + + K +L+  +K   + P A+  D+  L +  + 
Sbjct: 390 PQLKKELSSFIDKGQKLCADYSENTFTEFKKKLAEGLK--AQFPDASATDLQGLIDKRSD 447

Query: 842 ILSKCC 859
             SKCC
Sbjct: 448 FASKCC 453


>ref|XP_532404.2| PREDICTED: similar to Vitamin D-binding protein precursor (DBP)
           (Group-specific component) (Gc-globulin) (VDB) isoform 1
           [Canis familiaris].
          Length = 474

 Score =  572 bits (1473), Expect(2) = e-165
 Identities = 272/305 (89%), Positives = 289/305 (94%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 241
           MKRIL+FL AVAFVHALERGRDYEK+KVCKELA+LGKDDFTSLSMVLYSRKFPS TFEQI
Sbjct: 1   MKRILVFLFAVAFVHALERGRDYEKEKVCKELANLGKDDFTSLSMVLYSRKFPSSTFEQI 60

Query: 242 SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 421
           SHLVNEVVSLTE CCAEGADPDCYD+RTSALS +SCE DSPFPVHPGTAECCT+EGLE+K
Sbjct: 61  SHLVNEVVSLTETCCAEGADPDCYDHRTSALSARSCEKDSPFPVHPGTAECCTREGLERK 120

Query: 422 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 601
           LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPK FA +FMYEYSINYGQAPL+LLV YTK
Sbjct: 121 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKDFAEKFMYEYSINYGQAPLSLLVGYTK 180

Query: 602 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           SYLSMVGSCCTS SPT CFLKERLQ+KHLSLLT MSNR+CSQYAAYGK+KSRLSHLIKLA
Sbjct: 181 SYLSMVGSCCTSSSPTACFLKERLQMKHLSLLTTMSNRICSQYAAYGKEKSRLSHLIKLA 240

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QKVPTA LEDVLPLAE++ T+LSKCC+S SEDCMAKELPEYTVKICDNLS+KNSKF DCC
Sbjct: 241 QKVPTAELEDVLPLAEEINTVLSKCCESTSEDCMAKELPEYTVKICDNLSTKNSKFKDCC 300

Query: 962 QEKHP 976
           QEK P
Sbjct: 301 QEKTP 305



 Score = 30.8 bits (68), Expect(2) = e-165
 Identities = 11/13 (84%), Positives = 12/13 (92%)
 Frame = +1

Query: 967  KTPMDIFICTYFM 1005
            KTPMDIF+C YFM
Sbjct: 303  KTPMDIFVCAYFM 315



 Score = 68.2 bits (165), Expect = 1e-11
 Identities = 58/246 (23%), Positives = 104/246 (42%), Gaps = 5/246 (2%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           +++C + A+ GK+      ++  ++K P+   E +  L  E+ ++   CC E    DC  
Sbjct: 217 NRICSQYAAYGKEKSRLSHLIKLAQKVPTAELEDVLPLAEEINTVLSKCC-ESTSEDCMA 275

Query: 317 NRTSALSDKSCESDSPFPVHPGTAECCT-KEGLEKKLCMAALKHQPQ-EFPTYVEPTNDE 490
                 + K C++ S    +    +CC  K  ++  +C   +   P  E P    P N +
Sbjct: 276 KELPEYTVKICDNLST--KNSKFKDCCQEKTPMDIFVCAYFMPAAPTPELPAIELPANAD 333

Query: 491 ICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKER 670
           +C+   K      +Q+ +E S      P   L    +  L  +  CC S   T C   + 
Sbjct: 334 VCD---KGNTKAIDQYTFELS-RRTHLPEVFLSKILEPTLKSLAECCDSEDSTGCMNAQG 389

Query: 671 LQL-KHLSLLTIMSNRLCSQYA--AYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVAT 841
            QL K LS       +LC+ Y+   + + K +L+  +K   + P A+  D+  L +  + 
Sbjct: 390 PQLKKELSSFIDKGQKLCADYSENTFTEFKKKLAEGLK--AQFPDASATDLQGLIDKRSD 447

Query: 842 ILSKCC 859
             SKCC
Sbjct: 448 FASKCC 453


>ref|NP_001003026.1| serum albumin precursor [Canis lupus familiaris].
          Length = 608

 Score =  119 bits (298), Expect = 5e-27
 Identities = 73/299 (24%), Positives = 131/299 (43%), Gaps = 1/299 (0%)
 Frame = +2

Query: 74  LIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLV 253
           L FL + A+   L R   Y K ++      LG++ F  L +V +S+      FE    L 
Sbjct: 9   LFFLFSSAYSRGLVRREAY-KSEIAHRYNDLGEEHFRGLVLVAFSQYLQQCPFEDHVKLA 67

Query: 254 NEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMA 433
            EV    +AC AE +  +C  +  +   DK C   S    +   A+CC K+  ++  C  
Sbjct: 68  KEVTEFAKACAAEESGANCDKSLHTLFGDKLCTVASLRDKYGDMADCCEKQEPDRNECFL 127

Query: 434 ALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLS 613
           A K     FP  V P  D +C AF+ + + F  +++YE +  +       L+ Y + Y  
Sbjct: 128 AHKDDNPGFPPLVAPEPDALCAAFQDNEQLFLGKYLYEIARRHPYFYAPELLYYAQQYKG 187

Query: 614 MVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRL-CSQYAAYGKDKSRLSHLIKLAQKV 790
           +   CC +     C   +   L+   LL+    R  C+    +G    +   + +L+Q+ 
Sbjct: 188 VFAECCQAADKAACLGPKIEALREKVLLSSAKERFKCASLQKFGDRAFKAWSVARLSQRF 247

Query: 791 PTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCCQE 967
           P A+  ++  +  D+  +  +CC     +C A +  +    +C+N  S ++K  +CC +
Sbjct: 248 PKADFAEISKVVTDLTKVHKECCHGDLLEC-ADDRADLAKYMCENQDSISTKLKECCDK 305



 Score = 96.3 bits (238), Expect = 4e-20
 Identities = 69/288 (23%), Positives = 131/288 (45%), Gaps = 14/288 (4%)
 Frame = +2

Query: 146  CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
            C  L   G   F + S+   S++FP   F +IS +V ++  + + CC  G   +C D+R 
Sbjct: 224  CASLQKFGDRAFKAWSVARLSQRFPKADFAEISKVVTDLTKVHKECC-HGDLLECADDRA 282

Query: 326  SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQ--PQEFPT----YVEPTND 487
              L+   CE+     +     ECC K  LEK  C+A ++    P + P+    +VE  + 
Sbjct: 283  D-LAKYMCENQDS--ISTKLKECCDKPVLEKSQCLAEVERDELPGDLPSLAADFVE--DK 337

Query: 488  EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
            E+C+ +++    F   F+YEY+  + +  ++LL+   K Y + +  CC +  P  C+ K 
Sbjct: 338  EVCKNYQEAKDVFLGTFLYEYARRHPEYSVSLLLRLAKEYEATLEKCCATDDPPTCYAKV 397

Query: 668  RLQLKHL---SLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVA 838
              + K L       + +N  C  +   G+   + + L++  +K P  +   ++ ++  + 
Sbjct: 398  LDEFKPLVDEPQNLVKTN--CELFEKLGEYGFQNALLVRYTKKAPQVSTPTLVEVSRKLG 455

Query: 839  TILSKCC---DSASEDCMAKELPEYTVKIC--DNLSSKNSKFTDCCQE 967
             + +KCC   +S    C    L     ++C     +  + + T CC E
Sbjct: 456  KVGTKCCKKPESERMSCAEDFLSVVLNRLCVLHEKTPVSERVTKCCSE 503


>ref|XP_855557.1| PREDICTED: serum albumin [Canis familiaris].
          Length = 608

 Score =  119 bits (298), Expect = 5e-27
 Identities = 73/299 (24%), Positives = 131/299 (43%), Gaps = 1/299 (0%)
 Frame = +2

Query: 74  LIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLV 253
           L FL + A+   L R   Y K ++      LG++ F  L +V +S+      FE    L 
Sbjct: 9   LFFLFSSAYSRGLVRREAY-KSEIAHRYNDLGEEHFRGLVLVAFSQYLQQCPFEDHVKLA 67

Query: 254 NEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMA 433
            EV    +AC AE +  +C  +  +   DK C   S    +   A+CC K+  ++  C  
Sbjct: 68  KEVTEFAKACAAEESGANCDKSLHTLFGDKLCTVASLRDKYGDMADCCEKQEPDRNECFL 127

Query: 434 ALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLS 613
           A K     FP  V P  D +C AF+ + + F  +++YE +  +       L+ Y + Y  
Sbjct: 128 AHKDDNPGFPPLVAPEPDALCAAFQDNEQLFLGKYLYEIARRHPYFYAPELLYYAQQYKG 187

Query: 614 MVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRL-CSQYAAYGKDKSRLSHLIKLAQKV 790
           +   CC +     C   +   L+   LL+    R  C+    +G    +   + +L+Q+ 
Sbjct: 188 VFAECCQAADKAACLGPKIEALREKVLLSSAKERFKCASLQKFGDRAFKAWSVARLSQRF 247

Query: 791 PTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCCQE 967
           P A+  ++  +  D+  +  +CC     +C A +  +    +C+N  S ++K  +CC +
Sbjct: 248 PKADFAEISKVVTDLTKVHKECCHGDLLEC-ADDRADLAKYMCENQDSISTKLKECCDK 305



 Score = 97.1 bits (240), Expect = 2e-20
 Identities = 70/288 (24%), Positives = 131/288 (45%), Gaps = 14/288 (4%)
 Frame = +2

Query: 146  CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
            C  L   G   F + S+   S++FP   F +IS +V ++  + + CC  G   +C D+R 
Sbjct: 224  CASLQKFGDRAFKAWSVARLSQRFPKADFAEISKVVTDLTKVHKECC-HGDLLECADDRA 282

Query: 326  SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQ--PQEFPT----YVEPTND 487
              L+   CE+     +     ECC K  LEK  C+A ++    P + P+    +VE  + 
Sbjct: 283  D-LAKYMCENQDS--ISTKLKECCDKPVLEKSQCLAEVERDELPGDLPSLAADFVE--DK 337

Query: 488  EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
            E+C+ +++    F   F+YEYS  + +  ++LL+   K Y + +  CC +  P  C+ K 
Sbjct: 338  EVCKNYQEAKDVFLGTFLYEYSRRHPEYSVSLLLRLAKEYEATLEKCCATDDPPTCYAKV 397

Query: 668  RLQLKHL---SLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVA 838
              + K L       + +N  C  +   G+   + + L++  +K P  +   ++ ++  + 
Sbjct: 398  LDEFKPLVDEPQNLVKTN--CELFEKLGEYGFQNALLVRYTKKAPQVSTPTLVEVSRKLG 455

Query: 839  TILSKCC---DSASEDCMAKELPEYTVKIC--DNLSSKNSKFTDCCQE 967
             + +KCC   +S    C    L     ++C     +  + + T CC E
Sbjct: 456  KVGTKCCKKPESERMSCADDFLSVVLNRLCVLHEKTPVSERVTKCCSE 503


>ref|XP_532408.2| PREDICTED: similar to Afamin precursor (Alpha-albumin) (Alpha-Alb)
           [Canis familiaris].
          Length = 604

 Score = 97.1 bits (240), Expect = 2e-20
 Identities = 59/265 (22%), Positives = 115/265 (43%), Gaps = 2/265 (0%)
 Frame = +2

Query: 188 LSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPF 367
           ++++++++     +FE++  LV  +    + C  +   P C       L +  C  +   
Sbjct: 46  ITIIVFAQYVQEASFEEVEMLVKAMTEYRDKCLVDMTLPVCSKLANDVLLENICALEG-L 104

Query: 368 PVHPGTAECCTKEGLEKKLCMAALKHQPQEF-PTYVEPTNDEICEAFRKDPKGFANQFMY 544
           P     + CC+K  LE+KLC    K     F P       +E C+ ++ + + F N ++Y
Sbjct: 105 PQKHNFSHCCSKVDLERKLCFFHNKKADAGFLPPLPTLDPEEKCQTYKSNRESFLNNYIY 164

Query: 545 EYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LKERLQLKHLSLLTIMSNRLC 721
           E S          L++    +  M  +CC       CF  K  L +++L   +     +C
Sbjct: 165 EVSRRNPFVFAPTLLTVAARFEEMTKTCCEEQDKANCFRTKAELVIQYLKASSSFQKNVC 224

Query: 722 SQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPE 901
                +G       ++  L+QK P    +++  L EDV++  + CC+  +  C+ ++  +
Sbjct: 225 GALMKFGLQVLESINVAILSQKFPKIEFKELTSLLEDVSSKYNGCCEGDAVQCI-RDRSK 283

Query: 902 YTVKICDNLSSKNSKFTDCCQEKHP 976
               IC    S +SK  +CC++K P
Sbjct: 284 VMNHICSKQDSISSKIRECCEKKMP 308



 Score = 86.7 bits (213), Expect = 3e-17
 Identities = 63/298 (21%), Positives = 134/298 (44%), Gaps = 10/298 (3%)
 Frame = +2

Query: 101  VHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEA 280
            +  L+    ++K+ VC  L   G     S+++ + S+KFP   F++++ L+ +V S    
Sbjct: 210  IQYLKASSSFQKN-VCGALMKFGLQVLESINVAILSQKFPKIEFKELTSLLEDVSSKYNG 268

Query: 281  CCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALK--HQPQ 454
            CC EG    C  +R+  ++    + DS   +     ECC K+  E+  C+ +     +P+
Sbjct: 269  CC-EGDAVQCIRDRSKVMNHICSKQDS---ISSKIRECCEKKMPERGECIISSNKDDRPK 324

Query: 455  EF----PTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVG 622
            +       + E  N  +CE    +   F  +F+YE++  + +     L+     Y  ++ 
Sbjct: 325  DLGLREAKFTESEN--VCEERDANQTIFMAEFLYEHARRHPELSTPELLRIAGVYEELLK 382

Query: 623  SCCTSPSPTVCFLKERLQLKHLSLLTI-MSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTA 799
             CC + +P  C+ +   +    +  ++ +  R C  +   GKD  +  +++KL +  P  
Sbjct: 383  ECCHTENPPDCYRRAEYKFNETTEKSLKIVQRECEHFQNLGKDDLKYYYIVKLTKIAPQL 442

Query: 800  NLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTV-KICDNLSSK--NSKFTDCCQ 964
            + E++  L +++   L+ CC  + E      L +  + ++C    ++  N     CC+
Sbjct: 443  STEELTFLGKEMVAALTTCCTLSEEFACVDNLVDLVLGELCGINENRNINPDVDHCCK 500



 Score = 60.1 bits (144), Expect = 3e-09
 Identities = 39/182 (21%), Positives = 77/182 (42%), Gaps = 8/182 (4%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
           C+   +LGKDD     +V  ++  P  + E+++ L  E+V+    CC    +  C DN  
Sbjct: 416 CEHFQNLGKDDLKYYYIVKLTKIAPQLSTEELTFLGKEMVAALTTCCTLSEEFACVDNLV 475

Query: 326 SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPT-------- 481
             +  + C  +    ++P    CC      ++ C   L+       TYV P+        
Sbjct: 476 DLVLGELCGINENRNINPDVDHCCKTNFAFRRPCFEGLEAD----KTYVPPSTSQDLFTF 531

Query: 482 NDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFL 661
           + ++C+A  ++ +   ++F+        +     L S    + ++V +CC +  P  CF 
Sbjct: 532 HTDLCQAHNEELQRKKDRFLVNLVKRKPELTEEELRSLLADFSNVVVTCCKAEGPEACFN 591

Query: 662 KE 667
           +E
Sbjct: 592 EE 593


>ref|NP_001003027.1| alpha-fetoprotein precursor [Canis lupus familiaris].
          Length = 609

 Score = 95.1 bits (235), Expect = 9e-20
 Identities = 60/267 (22%), Positives = 121/267 (45%), Gaps = 4/267 (1%)
 Frame = +2

Query: 176 DFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCES 355
           +   L+ + +++     T++++S +V +++++ E          C +N+  A  ++ C  
Sbjct: 42  NLVDLATIFFAQFVQEATYKEVSKMVKDILTVIEKSTGSEQPGGCLENQLPAFLEEICH- 100

Query: 356 DSPFPVHPGTAECCTKEGLEKKLC-MAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFAN 532
           +       G A+CC++   E+  C +A  K  P   P +        C+A+ ++   F N
Sbjct: 101 EKEISEKYGLADCCSQREEERHNCFLAHKKAAPPSIPPFQVAEPVTSCKAYEENRDMFMN 160

Query: 533 QFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LKERLQLKHLSLLTIMS 709
           +++YE +  +       ++S    Y  ++  CC + +   CF  K  L  K L   ++++
Sbjct: 161 RYIYEIARRHPFLYAPTILSLAAHYGKIIPLCCKAENAVECFQTKTSLITKELRESSLLN 220

Query: 710 NRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCM-- 883
             +C+    +G    R   + KL+QK   AN  ++  L  DVA I  +CC     +C+  
Sbjct: 221 QHICAVMRNFGPRTFRAITVTKLSQKFSKANFTEIQKLVLDVAHIHEECCRGNVLECLQD 280

Query: 884 AKELPEYTVKICDNLSSKNSKFTDCCQ 964
            +++  Y   IC      +SK  DCC+
Sbjct: 281 GEKIMSY---ICSQQDILSSKIADCCK 304



 Score = 68.6 bits (166), Expect = 9e-12
 Identities = 55/252 (21%), Positives = 106/252 (42%), Gaps = 7/252 (2%)
 Frame = +2

Query: 143 VCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNR 322
           +C  + + G   F ++++   S+KF    F +I  LV +V  + E CC  G   +C  + 
Sbjct: 223 ICAVMRNFGPRTFRAITVTKLSQKFSKANFTEIQKLVLDVAHIHEECC-RGNVLECLQDG 281

Query: 323 TSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEA 502
              +S    + D    +    A+CC    LE   C+   ++  +  P  + P  +   E 
Sbjct: 282 EKIMSYICSQQDI---LSSKIADCCKLPILELGQCIIHAENDGK--PEGLSPNLNRFLEE 336

Query: 503 ------FRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLK 664
                   ++   F  +F YEYS  + +  + +++   K Y  ++  C  S +P  C  K
Sbjct: 337 RDFNQFSSREKDLFMARFTYEYSRRHTKLAVPVVLRVAKGYQELLEKCSQSENPLECQDK 396

Query: 665 ERLQL-KHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVAT 841
              +L K++     ++ R C  +   G+   + + L+   +K P     +++     +AT
Sbjct: 397 GEEELEKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTPPELMAFTRKMAT 456

Query: 842 ILSKCCDSASED 877
             + CC   SED
Sbjct: 457 AAATCC-QLSED 467


>ref|XP_854726.1| PREDICTED: similar to Afamin precursor (Alpha-albumin) (Alpha-Alb)
           [Canis familiaris].
          Length = 349

 Score = 75.5 bits (184), Expect = 8e-14
 Identities = 56/269 (20%), Positives = 111/269 (41%), Gaps = 7/269 (2%)
 Frame = +2

Query: 176 DFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCA---EGADPDCYDNRTSALSDKS 346
           D TS+ +  + +K    T+E++  +V E+V LT+ C       +  +C     +   +  
Sbjct: 38  DITSIMVAQFLQKL---TYEEVQTIVEELVDLTDKCKILKPHESPSECTHQLMTTFLEHI 94

Query: 347 CESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQP-QEFPTYVEPTNDEICEAFRKDPKG 523
           C +          ++CC      +  C    K     +   +  P  ++ICE  +++   
Sbjct: 95  CNNQGKAE-RQEFSDCCNINNKARLKCFLLYKKDDADDSDVFQIPNPEQICETHKENQVS 153

Query: 524 FANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LKERLQLKHLSLLT 700
              +++YE S  Y       +++ +  Y + + SCC   + T CF +K     K++  ++
Sbjct: 154 VKERYIYEMSRKYPFLYGPTILTMSACYETAIHSCCQEENKTECFQMKLEPIRKYIREIS 213

Query: 701 IMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDC 880
              + LC     +    ++   L+ L +K P  N  ++  LA  +  +   CC+     C
Sbjct: 214 WRHHHLCEIGIKFNHKVAKAVELVLLTKKQPKTNFSEIARLAVYIKNLHQTCCEGDVVAC 273

Query: 881 M--AKELPEYTVKICDNLSSKNSKFTDCC 961
           +    +L  YT   C + S  +SK   CC
Sbjct: 274 VLGRSQLMNYT---CSSQSIPSSKIAPCC 299


  Database: RefSeq49_CP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 18,874,504
  Number of sequences in database:  33,336
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 39,401,589
Number of extensions: 995029
Number of successful extensions: 2830
Number of sequences better than 1.0e-05: 7
Number of HSP's gapped: 2800
Number of HSP's successfully gapped: 16
Length of query: 335
Length of database: 18,874,504
Length adjustment: 103
Effective length of query: 232
Effective length of database: 15,440,896
Effective search space: 3582287872
Effective search space used: 3582287872
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008177
         (1006 letters)

Database: RefSeq49_SP.fasta 
           24,897 sequences; 11,343,932 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|XP_003357019.1| PREDICTED: vitamin D-binding protein [Sus sc...   623   0.0  
Alignment   gi|NP_001005208.1| serum albumin precursor [Sus scrofa].             114   9e-26
Alignment   gi|NP_999482.1| alpha-fetoprotein precursor [Sus scrofa].            104   7e-23
Alignment   gi|XP_003357020.1| PREDICTED: afamin [Sus scrofa].                    97   1e-20

>ref|XP_003357019.1| PREDICTED: vitamin D-binding protein [Sus scrofa].
          Length = 473

 Score =  623 bits (1606), Expect(2) = 0.0
 Identities = 304/305 (99%), Positives = 304/305 (99%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 241
           MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI
Sbjct: 1   MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 60

Query: 242 SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 421
           SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK
Sbjct: 61  SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 120

Query: 422 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 601
           LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK
Sbjct: 121 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 180

Query: 602 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA
Sbjct: 181 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 240

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC
Sbjct: 241 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 300

Query: 962 QEKHP 976
           QEK P
Sbjct: 301 QEKTP 305



 Score = 33.1 bits (74), Expect(2) = 0.0
 Identities = 13/13 (100%), Positives = 13/13 (100%)
 Frame = +1

Query: 967  KTPMDIFICTYFM 1005
            KTPMDIFICTYFM
Sbjct: 303  KTPMDIFICTYFM 315



 Score = 75.1 bits (183), Expect = 6e-14
 Identities = 61/246 (24%), Positives = 109/246 (44%), Gaps = 5/246 (2%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           +++C + A+ GKD      ++  ++K P+   E +  L  +V ++   CC + A  DC  
Sbjct: 217 NRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCC-DSASEDCMA 275

Query: 317 NRTSALSDKSCESDSPFPVHPGTAECCT-KEGLEKKLCMAALKHQPQ-EFPTYVEPTNDE 490
                 + K C++ S    +    +CC  K  ++  +C   +   P+ E P    PTN +
Sbjct: 276 KELPEYTVKICDNLSS--KNSKFTDCCQEKTPMDIFICTYFMPAAPRPELPDVKLPTNKD 333

Query: 491 ICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKER 670
           +C+  + +PK   +Q+++E S      P   L    +  L  +  CC S   T CF  + 
Sbjct: 334 VCD--KGNPK-VLDQYIFELS-RKTHIPEVFLSKILEPTLKSLDECCHSEDSTACFKAKG 389

Query: 671 LQL-KHLSLLTIMSNRLCSQYA--AYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVAT 841
            Q  K LS        LC+ Y+   + + K +L+   +L  K P A   ++  L +  + 
Sbjct: 390 PQFKKELSSFIEKGQELCADYSENTFTEYKKKLAE--RLRGKWPDATETELEELVKKRSD 447

Query: 842 ILSKCC 859
             SKCC
Sbjct: 448 FASKCC 453


>ref|NP_001005208.1| serum albumin precursor [Sus scrofa].
          Length = 607

 Score =  114 bits (285), Expect = 9e-26
 Identities = 71/299 (23%), Positives = 135/299 (45%), Gaps = 1/299 (0%)
 Frame = +2

Query: 74  LIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLV 253
           L+FL + A+   + R RD  K ++      LG+  F  L ++ +S+      +E+   LV
Sbjct: 9   LLFLFSSAYSRGVFR-RDTYKSEIAHRFKDLGEQYFKGLVLIAFSQHLQQCPYEEHVKLV 67

Query: 254 NEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMA 433
            EV    + C A+ +  +C  +  +   DK C   S    +   A+CC KE  E+  C  
Sbjct: 68  REVTEFAKTCVADESAENCDKSIHTLFGDKLCAIPSLREHYGDLADCCEKEEPERNECFL 127

Query: 434 ALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLS 613
             K+   + P  ++P    +C  F++D + F  +++YE +  +       L+ Y   Y  
Sbjct: 128 QHKNDNPDIPK-LKPDPVALCADFQEDEQKFWGKYLYEIARRHPYFYAPELLYYAIIYKD 186

Query: 614 MVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRL-CSQYAAYGKDKSRLSHLIKLAQKV 790
           +   CC +     C L +   L+   L +    RL C+    +G+   +   L +L+Q+ 
Sbjct: 187 VFSECCQAADKAACLLPKIEHLREKVLTSAAKQRLKCASIQKFGERAFKAWSLARLSQRF 246

Query: 791 PTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCCQE 967
           P A+  ++  +  D+A +  +CC     +C A +  +    IC+N  + ++K  +CC +
Sbjct: 247 PKADFTEISKIVTDLAKVHKECCHGDLLEC-ADDRADLAKYICENQDTISTKLKECCDK 304



 Score = 95.9 bits (237), Expect = 3e-20
 Identities = 70/288 (24%), Positives = 126/288 (43%), Gaps = 14/288 (4%)
 Frame = +2

Query: 146  CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
            C  +   G+  F + S+   S++FP   F +IS +V ++  + + CC  G   +C D+R 
Sbjct: 223  CASIQKFGERAFKAWSLARLSQRFPKADFTEISKIVTDLAKVHKECC-HGDLLECADDRA 281

Query: 326  SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPT------ND 487
              L+   CE+     +     ECC K  LEK  C+A  K    E P  + P       + 
Sbjct: 282  D-LAKYICENQDT--ISTKLKECCDKPLLEKSHCIAEAKRD--ELPADLNPLEHDFVEDK 336

Query: 488  EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
            E+C+ +++    F   F+YEYS  +    ++LL+   K Y + +  CC    P  C+   
Sbjct: 337  EVCKNYKEAKHVFLGTFLYEYSRRHPDYSVSLLLRIAKIYEATLEDCCAKEDPPACY--A 394

Query: 668  RLQLKHLSLLTIMSNRL---CSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVA 838
             +  K   L+    N +   C  +   G+   + + +++  +KVP  +   ++ +A  + 
Sbjct: 395  TVFDKFQPLVDEPKNLIKQNCELFEKLGEYGFQNALIVRYTKKVPQVSTPTLVEVARKLG 454

Query: 839  TILSKCCDSASED---CMAKELPEYTVKIC--DNLSSKNSKFTDCCQE 967
             + S+CC    E+   C    L     ++C     +  + K T CC E
Sbjct: 455  LVGSRCCKRPEEERLSCAEDYLSLVLNRLCVLHEKTPVSEKVTKCCTE 502



 Score = 50.8 bits (120), Expect = 1e-06
 Identities = 38/181 (20%), Positives = 76/181 (41%), Gaps = 7/181 (3%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADP--DCYDN 319
           C+    LG+  F +  +V Y++K P  +   +  +  ++  +   CC    +    C ++
Sbjct: 415 CELFEKLGEYGFQNALIVRYTKKVPQVSTPTLVEVARKLGLVGSRCCKRPEEERLSCAED 474

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALK----HQPQEFPTYVEPTND 487
             S + ++ C      PV     +CCT+  + ++ C +AL     ++P+EF       + 
Sbjct: 475 YLSLVLNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYKPKEFVEGTFTFHA 534

Query: 488 EICEAFRKDPKGFANQF-MYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLK 664
           ++C    +D K    Q  + E   +   A    L +   ++ + V  CC +P    CF  
Sbjct: 535 DLC-TLPEDEKQIKKQTALVELLKHKPHATEEQLRTVLGNFAAFVQKCCAAPDHEACFAV 593

Query: 665 E 667
           E
Sbjct: 594 E 594


>ref|NP_999482.1| alpha-fetoprotein precursor [Sus scrofa].
          Length = 610

 Score =  104 bits (260), Expect = 7e-23
 Identities = 57/265 (21%), Positives = 123/265 (46%), Gaps = 2/265 (0%)
 Frame = +2

Query: 176 DFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCES 355
           +   L+ + +++     T+++++ +V +V+++ E          C +N+ S   ++ C  
Sbjct: 42  NLVDLATIFFAQFVQEATYKEVNQMVKDVLTVIEKSTGSEQPAGCLENQVSVFLEEICHE 101

Query: 356 DSPFPVHPGTAECCTKEGLEKKLC-MAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFAN 532
           +   P   G + CC++ G E+  C +A  K  P   P +  P     C+A+ ++ + F  
Sbjct: 102 EE-IPEKYGLSHCCSQSGEERHNCFLARKKAAPASIPPFQVPEPVTSCKAYEENRELFMT 160

Query: 533 QFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERLQL-KHLSLLTIMS 709
           +++YE +  +       ++S    Y  ++  CC + +   CF  +   + K L   ++++
Sbjct: 161 RYIYEIARRHPFLYAPTILSLAAQYDKIIPPCCKAENAVECFQTKAASITKELRESSLLN 220

Query: 710 NRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAK 889
             +C+    +G    R   + KL+QK P AN  ++  L  DVA I  +CC     +C+ +
Sbjct: 221 QHMCTVMRQFGARTFRAITVTKLSQKFPKANFTEIQKLVLDVAHIHEECCRGNVLECL-Q 279

Query: 890 ELPEYTVKICDNLSSKNSKFTDCCQ 964
           +       +C    + +SK  +CC+
Sbjct: 280 DAERVVSYVCSQQDTLSSKIAECCK 304



 Score = 67.0 bits (162), Expect = 2e-11
 Identities = 55/252 (21%), Positives = 106/252 (42%), Gaps = 7/252 (2%)
 Frame = +2

Query: 143 VCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNR 322
           +C  +   G   F ++++   S+KFP   F +I  LV +V  + E CC  G   +C  + 
Sbjct: 223 MCTVMRQFGARTFRAITVTKLSQKFPKANFTEIQKLVLDVAHIHEECC-RGNVLECLQDA 281

Query: 323 TSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPT------N 484
              +S    + D+   +    AECC K     +L    +  +  + P  + P        
Sbjct: 282 ERVVSYVCSQQDT---LSSKIAECC-KLPTTLELGQCIIHAENDDKPEGLSPNLNRFLGE 337

Query: 485 DEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLK 664
            +  +   ++      +F YEYS  + +  + +++   K Y  ++  C  S +P  C  K
Sbjct: 338 RDFNQLSSREKDLSMARFTYEYSRRHPKLAVPVILRVAKGYQELLEKCSQSENPLECQDK 397

Query: 665 ERLQL-KHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVAT 841
              +L K++     ++ R C  +   G+   + + L+   +K P     +++ L   +AT
Sbjct: 398 GEEELEKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTPPELMALTRKMAT 457

Query: 842 ILSKCCDSASED 877
             + CC   SED
Sbjct: 458 TGAACC-HLSED 468



 Score = 55.1 bits (131), Expect = 6e-08
 Identities = 39/184 (21%), Positives = 71/184 (38%), Gaps = 10/184 (5%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACC--AEGADPDCYDN 319
           C     LG+    +  +V Y++K P  T  ++  L  ++ +   ACC  +E     C + 
Sbjct: 417 CGLFQKLGEYYLQNAFLVAYTKKAPQLTPPELMALTRKMATTGAACCHLSEDRQLACGEG 476

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPT------ 481
               +  + C      P++PG  +CCT     ++ C ++L        TYV P       
Sbjct: 477 AADLIIGQLCIRHEEMPINPGVGQCCTSSYANRRPCFSSLVLD----ETYVPPPFSDDKF 532

Query: 482 --NDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVC 655
             + ++C+A     +    QF+        Q     L +    +  ++  CC      VC
Sbjct: 533 IFHKDLCQAQGVALQTMKQQFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVC 592

Query: 656 FLKE 667
           F +E
Sbjct: 593 FAEE 596


>ref|XP_003357020.1| PREDICTED: afamin [Sus scrofa].
          Length = 604

 Score = 97.4 bits (241), Expect = 1e-20
 Identities = 70/302 (23%), Positives = 134/302 (44%), Gaps = 10/302 (3%)
 Frame = +2

Query: 89   AVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVS 268
            A  F+  L+    Y+K+ VC  L   G     S+++ + S+KFP   F+Q++ L+ ++ S
Sbjct: 206  AEPFIQYLKALSSYQKN-VCGALLKFGPQILQSINIAVLSQKFPKIEFKQLTSLLEDISS 264

Query: 269  LTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQ 448
              + CC  G    C   R+  +S    + DS   +     ECC K+  E+  C+  +   
Sbjct: 265  KYDGCCG-GDVVHCIRGRSKVMSHICSKQDS---ISSKIKECCEKKIPERGECI--IYSN 318

Query: 449  PQEFPTYVEPT------NDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYL 610
              + P  + P       ++ +CE    DP+ F  +F+YEYS  + +     L+     Y 
Sbjct: 319  KDDRPNDLSPREAKFTESENVCEERDADPETFLAEFLYEYSRRHPELSTPELLRIAGVYE 378

Query: 611  SMVGSCCTSPSPTVCFLKERLQLKHLSLLTI-MSNRLCSQYAAYGKDKSRLSHLIKLAQK 787
             ++  CC S +P  C+     +    +  ++ +  R C  +   GKD  +  +L +L + 
Sbjct: 379  DLLRECCNSQTPPDCYKDAEKKFNETTEKSLKIVQRECEHFQNLGKDDLKYHYLARLTKI 438

Query: 788  VPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTV-KIC--DNLSSKNSKFTDC 958
             P  + +++  L +++   L+ CC  + E      L +  + ++C  +   S N     C
Sbjct: 439  APQLSTDELTFLGKEMMIALATCCTLSEEFACVDNLMDLVLGELCGINKNRSINPTVDHC 498

Query: 959  CQ 964
            C+
Sbjct: 499  CK 500



 Score = 91.3 bits (225), Expect = 8e-19
 Identities = 58/271 (21%), Positives = 113/271 (41%), Gaps = 2/271 (0%)
 Frame = +2

Query: 170 KDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSC 349
           +D+   ++++ +++     +FE++  LV  +    + C A+   P+C       L +  C
Sbjct: 40  EDNVVYITIIAFAQYIQEASFEEVEMLVKAMKEYRDRCLADMTLPECSKLANDVLLENIC 99

Query: 350 ESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEF-PTYVEPTNDEICEAFRKDPKGF 526
             +   P     + CC K   E++LC    K     F P       +E C+ ++ + + F
Sbjct: 100 AMEG-LPQKYNFSHCCHKVDFERRLCFLHNKKAEVGFLPPLPTLDPEEKCQTYKTNKESF 158

Query: 527 ANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LKERLQLKHLSLLTI 703
            N ++YE S          L++    +  M  +CC       CF  K    +++L  L+ 
Sbjct: 159 LNNYIYEVSRRNPFVFAPTLLTVAARFEEMAETCCEEQEKANCFRSKAEPFIQYLKALSS 218

Query: 704 MSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCM 883
               +C     +G    +  ++  L+QK P    + +  L ED+++    CC      C+
Sbjct: 219 YQKNVCGALLKFGPQILQSINIAVLSQKFPKIEFKQLTSLLEDISSKYDGCCGGDVVHCI 278

Query: 884 AKELPEYTVKICDNLSSKNSKFTDCCQEKHP 976
            +   +    IC    S +SK  +CC++K P
Sbjct: 279 -RGRSKVMSHICSKQDSISSKIKECCEKKIP 308



 Score = 49.7 bits (117), Expect = 3e-06
 Identities = 34/180 (18%), Positives = 72/180 (40%), Gaps = 6/180 (3%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
           C+   +LGKDD     +   ++  P  + ++++ L  E++     CC    +  C DN  
Sbjct: 416 CEHFQNLGKDDLKYHYLARLTKIAPQLSTDELTFLGKEMMIALATCCTLSEEFACVDNLM 475

Query: 326 SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTN------D 487
             +  + C  +    ++P    CC      ++ C   L+      P  + P+        
Sbjct: 476 DLVLGELCGINKNRSINPTVDHCCKTNFAFRRSCFEGLEADKTYVP--LPPSQGLFTFPP 533

Query: 488 EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
           ++C+A  ++ +   ++F+        +     L S    + ++V  CC +  P  CF +E
Sbjct: 534 DLCQAHNEELQRKKDRFLVNLVKLKPELTEEALRSSLIEFTNVVVKCCQAEGPEACFEEE 593


  Database: RefSeq49_SP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 11,343,932
  Number of sequences in database:  24,897
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 24,344,106
Number of extensions: 622599
Number of successful extensions: 1915
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 1891
Number of HSP's successfully gapped: 12
Length of query: 335
Length of database: 11,343,932
Length adjustment: 99
Effective length of query: 236
Effective length of database: 8,879,129
Effective search space: 2095474444
Effective search space used: 2095474444
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008177
         (1006 letters)

Database: RefSeq49_MP.fasta 
           30,036 sequences; 15,617,559 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|NP_032122.1| vitamin D-binding protein precursor [Mus muscul...   528   e-152
Alignment   gi|NP_033784.2| serum albumin precursor [Mus musculus].              117   1e-26
Alignment   gi|NP_031449.3| alpha-fetoprotein precursor [Mus musculus].          109   3e-24
Alignment   gi|NP_660128.2| afamin precursor [Mus musculus].                     105   7e-23
Alignment   gi|XP_891828.4| PREDICTED: alpha-fetoprotein, partial [Mus musc...    80   3e-15
Alignment   gi|XP_979506.2| PREDICTED: alpha-fetoprotein, partial [Mus musc...    80   3e-15

>ref|NP_032122.1| vitamin D-binding protein precursor [Mus musculus].
          Length = 476

 Score =  528 bits (1360), Expect(2) = e-152
 Identities = 249/305 (81%), Positives = 275/305 (90%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 241
           MKR+L+ LLA+AF HALERGRDYEKDKVC ELA LGK+DF SLS++LYSRKF S TFEQ+
Sbjct: 1   MKRVLVLLLALAFGHALERGRDYEKDKVCNELAMLGKEDFRSLSLILYSRKFSSSTFEQV 60

Query: 242 SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 421
           + LV EVVSLTE CCAEGADP CYD RTS LS KSCESD+PFPVHPGT ECCTKEGLE+K
Sbjct: 61  NQLVKEVVSLTEECCAEGADPTCYDTRTSELSVKSCESDAPFPVHPGTPECCTKEGLERK 120

Query: 422 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 601
           LCMAAL HQPQEFPTYVEPTNDEICEAFR+DPKGFA+QF+YEYS NYGQAPL LLV+YTK
Sbjct: 121 LCMAALSHQPQEFPTYVEPTNDEICEAFRRDPKGFADQFLYEYSSNYGQAPLPLLVAYTK 180

Query: 602 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           +YLSMVGSCCTS +PTVCF+KERLQ+KHLSLLT MSNR+CSQYAAYGK+KSRLSHLIKLA
Sbjct: 181 NYLSMVGSCCTSANPTVCFVKERLQMKHLSLLTTMSNRVCSQYAAYGKEKSRLSHLIKLA 240

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QKVPTANLE+VLPLAED   ILS+CC+S SEDCMA ELPE+T+KIC NLS KNSKF +CC
Sbjct: 241 QKVPTANLENVLPLAEDFTEILSRCCESTSEDCMASELPEHTIKICQNLSKKNSKFEECC 300

Query: 962 QEKHP 976
           QE  P
Sbjct: 301 QENTP 305



 Score = 28.1 bits (61), Expect(2) = e-152
 Identities = 10/12 (83%), Positives = 12/12 (100%)
 Frame = +1

Query: 970  TPMDIFICTYFM 1005
            TPM+IF+CTYFM
Sbjct: 304  TPMNIFMCTYFM 315



 Score = 62.4 bits (150), Expect = 5e-10
 Identities = 53/247 (21%), Positives = 99/247 (40%), Gaps = 6/247 (2%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           ++VC + A+ GK+      ++  ++K P+   E +  L  +   +   CC E    DC  
Sbjct: 217 NRVCSQYAAYGKEKSRLSHLIKLAQKVPTANLENVLPLAEDFTEILSRCC-ESTSEDCMA 275

Query: 317 NRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAAL---KHQPQEFPTYVEPTND 487
           +     + K C++ S    +    ECC +E     + M        +P + P    PT  
Sbjct: 276 SELPEHTIKICQNLSK--KNSKFEECC-QENTPMNIFMCTYFMPAAEPLQLPAIKLPTGK 332

Query: 488 EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
           ++C    +      +Q+ +E S    Q P   L    +  L  +  CC +     CF  +
Sbjct: 333 DLCG---QSTTQAMDQYTFELS-RRTQVPEVFLSKVLEPTLKTLRECCDTQDSVACFSTQ 388

Query: 668 RLQLK-HLSLLTIMSNRLCSQYA--AYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVA 838
              LK  L+        +C+ Y+   + + K +L+   +L  K P  +  ++  + E  +
Sbjct: 389 SPLLKRQLTSFIEKGQEMCADYSENTFTEYKKKLAE--RLRTKTPNTSPAELKDMVEKHS 446

Query: 839 TILSKCC 859
              SKCC
Sbjct: 447 DFASKCC 453


>ref|NP_033784.2| serum albumin precursor [Mus musculus].
          Length = 608

 Score =  117 bits (294), Expect = 1e-26
 Identities = 67/300 (22%), Positives = 138/300 (46%), Gaps = 1/300 (0%)
 Frame = +2

Query: 71  ILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHL 250
           +L+F+   AF   + R R+  K ++      LG+  F  L ++ +S+     ++++ + L
Sbjct: 8   LLLFVSGSAFSRGVFR-REAHKSEIAHRYNDLGEQHFKGLVLIAFSQYLQKCSYDEHAKL 66

Query: 251 VNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCM 430
           V EV    + C A+ +  +C  +  +   DK C   +    +   A+CCTK+  E+  C 
Sbjct: 67  VQEVTDFAKTCVADESAANCDKSLHTLFGDKLCAIPNLRENYGELADCCTKQEPERNECF 126

Query: 431 AALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYL 610
              K      P +  P  + +C +F+++P  F   +++E +  +       L+ Y + Y 
Sbjct: 127 LQHKDDNPSLPPFERPEAEAMCTSFKENPTTFMGHYLHEVARRHPYFYAPELLYYAEQYN 186

Query: 611 SMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRL-CSQYAAYGKDKSRLSHLIKLAQK 787
            ++  CC       C   +   +K  +L++ +  R+ CS    +G+   +   + +L+Q 
Sbjct: 187 EILTQCCAEADKESCLTPKLDGVKEKALVSSVRQRMKCSSMQKFGERAFKAWAVARLSQT 246

Query: 788 VPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCCQE 967
            P A+  ++  LA D+  +  +CC     +C A +  E    +C+N ++ +SK   CC +
Sbjct: 247 FPNADFAEITKLATDLTKVNKECCHGDLLEC-ADDRAELAKYMCENQATISSKLQTCCDK 305



 Score = 91.3 bits (225), Expect = 1e-18
 Identities = 67/286 (23%), Positives = 126/286 (44%), Gaps = 14/286 (4%)
 Frame = +2

Query: 146  CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
            C  +   G+  F + ++   S+ FP+  F +I+ L  ++  + + CC  G   +C D+R 
Sbjct: 224  CSSMQKFGERAFKAWAVARLSQTFPNADFAEITKLATDLTKVNKECC-HGDLLECADDRA 282

Query: 326  SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQ--PQEFPT----YVEPTND 487
              L+   CE+ +       T  CC K  L+K  C++ ++H   P + P     +VE  + 
Sbjct: 283  E-LAKYMCENQATISSKLQT--CCDKPLLKKAHCLSEVEHDTMPADLPAIAADFVE--DQ 337

Query: 488  EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF--- 658
            E+C+ + +    F   F+YEYS  +    ++LL+   K Y + +  CC   +P  C+   
Sbjct: 338  EVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEANPPACYGTV 397

Query: 659  LKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVA 838
            L E   L       + +N  C  Y   G+   + + L++  QK P  +   ++  A ++ 
Sbjct: 398  LAEFQPLVEEPKNLVKTN--CDLYEKLGEYGFQNAILVRYTQKAPQVSTPTLVEAARNLG 455

Query: 839  TILSKCCDSASED---CMAKELPEYTVKIC--DNLSSKNSKFTDCC 961
             + +KCC    +    C+   L     ++C     +  +   T CC
Sbjct: 456  RVGTKCCTLPEDQRLPCVEDYLSAILNRVCLLHEKTPVSEHVTKCC 501



 Score = 50.8 bits (120), Expect = 2e-06
 Identities = 37/180 (20%), Positives = 71/180 (39%), Gaps = 6/180 (3%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADP--DCYDN 319
           C     LG+  F +  +V Y++K P  +   +      +  +   CC    D    C ++
Sbjct: 416 CDLYEKLGEYGFQNAILVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEDQRLPCVED 475

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALK----HQPQEFPTYVEPTND 487
             SA+ ++ C      PV     +CC+   +E++ C +AL     + P+EF       + 
Sbjct: 476 YLSAILNRVCLLHEKTPVSEHVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHS 535

Query: 488 EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
           +IC    K+ +      + E   +  +A    L +    +   + +CC +     CF  E
Sbjct: 536 DICTLPEKEKQIKKQTALAELVKHKPKATAEQLKTVMDDFAQFLDTCCKAADKDTCFSTE 595


>ref|NP_031449.3| alpha-fetoprotein precursor [Mus musculus].
          Length = 605

 Score =  109 bits (273), Expect = 3e-24
 Identities = 70/266 (26%), Positives = 133/266 (50%), Gaps = 6/266 (2%)
 Frame = +2

Query: 185 SLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSP 364
           S++ + +++  P  T E+++ + ++V++  +    +G    C +++ S   D+ C  ++ 
Sbjct: 45  SIATITFTQFVPEATEEEVNKMTSDVLAAMKKNSGDG----CLESQLSVFLDEICH-ETE 99

Query: 365 FPVHPGTAECCTKEGLEKKLCMAALKHQ-PQEFPTYVEPTNDEICEAFRKDPKGFANQFM 541
                G + CC++ G+E+  C+ A K   P   P +  P   E C+A  ++   F N+F+
Sbjct: 100 LSNKYGLSGCCSQSGVERHQCLLARKKTAPASVPPFQFPEPAESCKAHEENRAVFMNRFI 159

Query: 542 YEYS----INYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERLQL-KHLSLLTIM 706
           YE S      Y  A L+L   Y K  L+    CC + +   CF  +R  + K L   +++
Sbjct: 160 YEVSRRNPFMYAPAILSLAAQYDKVVLA----CCKADNKEECFQTKRASIAKELREGSML 215

Query: 707 SNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMA 886
           +  +CS    +G    + + +IKL+QK+  AN  ++  LA DVA I  +CC   S +C+ 
Sbjct: 216 NEHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECCQGNSLECL- 274

Query: 887 KELPEYTVKICDNLSSKNSKFTDCCQ 964
           ++  +    IC   +  +SK  +CC+
Sbjct: 275 QDGEKVMTYICSQQNILSSKIAECCK 300



 Score = 73.6 bits (179), Expect = 2e-13
 Identities = 62/290 (21%), Positives = 120/290 (41%), Gaps = 11/290 (3%)
 Frame = +2

Query: 137  DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
            + VC  +   G  +  + +++  S+K     F +I  L  +V  + E CC +G   +C  
Sbjct: 217  EHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECC-QGNSLECLQ 275

Query: 317  NRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTN---D 487
            +    ++    + +    +    AECC    ++   C+   ++  +     + P+    D
Sbjct: 276  DGEKVMTYICSQQNI---LSSKIAECCKLPMIQLGFCIIHAENGVKPEGLSLNPSQFLGD 332

Query: 488  EICEAFRKDPK-GFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-- 658
                 F  + K  F   F++EYS  +   P+++++   K+Y  ++  C  S +   C   
Sbjct: 333  RNFAQFSSEEKIMFMASFLHEYSRTHPNLPVSVILRIAKTYQEILEKCSQSGNLPGCQDN 392

Query: 659  LKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVA 838
            L+E LQ KH+     +S + C+ Y   G  K +   LI   +K P     +++ L   + 
Sbjct: 393  LEEELQ-KHIEESQALSKQSCALYQTLGDYKLQNLFLIGYTRKAPQLTSAELIDLTGKMV 451

Query: 839  TILSKCCDSASE---DCMAKELPEYTVKIC--DNLSSKNSKFTDCCQEKH 973
            +I S CC  + E    C       +   +C  +  S  NS  + CC   +
Sbjct: 452  SIASTCCQLSEEKWSGCGEGMADIFIGHLCIRNEASPVNSGISHCCNSSY 501



 Score = 57.8 bits (138), Expect = 1e-08
 Identities = 47/184 (25%), Positives = 74/184 (40%), Gaps = 10/184 (5%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACC--AEGADPDCYDN 319
           C    +LG     +L ++ Y+RK P  T  ++  L  ++VS+   CC  +E     C + 
Sbjct: 412 CALYQTLGDYKLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASTCCQLSEEKWSGCGEG 471

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICE 499
                    C  +   PV+ G + CC      ++LC+ +         TY  P   E   
Sbjct: 472 MADIFIGHLCIRNEASPVNSGISHCCNSSYSNRRLCITSFLRD----ETYAPPPFSEDKF 527

Query: 500 AFRKD---PKGFANQFM-YEYSINYGQAPLTL----LVSYTKSYLSMVGSCCTSPSPTVC 655
            F KD    +G A Q M  E  IN  +    L    L + T  +  ++  CC +    VC
Sbjct: 528 IFHKDLCQAQGKALQTMKQELLINLVKQKPELTEEQLAAVTADFSGLLEKCCKAQDQEVC 587

Query: 656 FLKE 667
           F +E
Sbjct: 588 FTEE 591


>ref|NP_660128.2| afamin precursor [Mus musculus].
          Length = 608

 Score =  105 bits (261), Expect = 7e-23
 Identities = 71/300 (23%), Positives = 139/300 (46%), Gaps = 8/300 (2%)
 Frame = +2

Query: 89   AVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVS 268
            A      L+    Y+++ VC  L   G     S+++ ++S+KFP   F+ ++ L+ +V S
Sbjct: 206  AAPITQYLKASSSYQRN-VCGALIKFGPKVLNSINVAVFSKKFPKIGFKDLTTLLEDVSS 264

Query: 269  LTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMA-ALKH 445
            + E CC EG    C  +++  ++    + DS   +      CC K+ LE++ C+  A K 
Sbjct: 265  MYEGCC-EGDVVHCIRSQSQVVNHICSKQDS---ISSKIKVCCEKKTLEREACIINANKD 320

Query: 446  QPQEFPTYVEPT---NDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSM 616
               E  +  E     ++ +C+    DP  F  +F+YEYS  +       L+  TK Y+  
Sbjct: 321  DRPEGLSLREAKFTESENVCQERDSDPDKFFAEFIYEYSRRHPDLSTPELLRITKVYMDF 380

Query: 617  VGSCCTSPSPTVCFLKERLQLKHLSLLTI-MSNRLCSQYAAYGKDKSRLSHLIKLAQKVP 793
            +  CC+  +P  C+     +    +  ++ M  + C Q+   GKD  +   L+K  +  P
Sbjct: 381  LEDCCSRENPAGCYRHVEDKFNETTQRSLAMVQQECKQFQELGKDTLQRHFLVKFTKAAP 440

Query: 794  TANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTV-KICDNLSSK--NSKFTDCCQ 964
               +E+++ L++++   L+ CC  + E      L +  + ++C   +++  N     CC+
Sbjct: 441  QLPMEELVSLSKEMVAALTTCCTLSDEFACVDNLADLVLGELCGVNTNRTINPAVDHCCK 500



 Score = 95.1 bits (235), Expect = 7e-20
 Identities = 56/268 (20%), Positives = 116/268 (43%), Gaps = 2/268 (0%)
 Frame = +2

Query: 173 DDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCE 352
           ++ T L+++ +S+     +F+++  LV  ++   + C A+   P+C      A+ D  C+
Sbjct: 41  ENTTYLAIIAFSQYVQEASFDEVETLVKVMLDYRDRCWADNTLPECSKTANDAIQDMLCD 100

Query: 353 SDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEF-PTYVEPTNDEICEAFRKDPKGFA 529
            +   P     + CC K G  ++LC    K     F P +     +E C+A++ + + F 
Sbjct: 101 MEG-LPQKHNFSHCCGKAGFPRRLCFFYNKKANVGFLPPFPTLDPEEKCQAYKNNSESFL 159

Query: 530 NQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERLQL-KHLSLLTIM 706
           + +MYE +         +L++    +     +CC       CF  +   + ++L   +  
Sbjct: 160 HLYMYEVARRNPFVFAPVLLAVAAWFEEAATTCCEQQQKATCFQAKAAPITQYLKASSSY 219

Query: 707 SNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMA 886
              +C     +G       ++   ++K P    +D+  L EDV+++   CC+     C+ 
Sbjct: 220 QRNVCGALIKFGPKVLNSINVAVFSKKFPKIGFKDLTTLLEDVSSMYEGCCEGDVVHCIR 279

Query: 887 KELPEYTVKICDNLSSKNSKFTDCCQEK 970
            +  +    IC    S +SK   CC++K
Sbjct: 280 SQ-SQVVNHICSKQDSISSKIKVCCEKK 306



 Score = 49.3 bits (116), Expect = 5e-06
 Identities = 29/123 (23%), Positives = 50/123 (40%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
           CK+   LGKD      +V +++  P    E++  L  E+V+    CC    +  C DN  
Sbjct: 416 CKQFQELGKDTLQRHFLVKFTKAAPQLPMEELVSLSKEMVAALTTCCTLSDEFACVDNLA 475

Query: 326 SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAF 505
             +  + C  ++   ++P    CC  +   ++ C   LK       TY  P+   +  A 
Sbjct: 476 DLVLGELCGVNTNRTINPAVDHCCKTDFAFRRHCFEHLKAD----TTYELPSVSALVSAL 531

Query: 506 RKD 514
             D
Sbjct: 532 HTD 534


>ref|XP_891828.4| PREDICTED: alpha-fetoprotein, partial [Mus musculus].
          Length = 363

 Score = 79.7 bits (195), Expect = 3e-15
 Identities = 58/267 (21%), Positives = 111/267 (41%), Gaps = 6/267 (2%)
 Frame = +2

Query: 197 VLYSRKFPSGTFEQISHLVNEVVSLTEAC--CAEGADPDCYDNRTSALSDKSCESDSPFP 370
           ++ ++      ++Q+  +  E++ L E C      +  +C     +A     C +     
Sbjct: 57  IMVAQFLQDAAYDQVQTIAKELLKLAEKCKRLKPESPSECAHQLVAAFLIHICNNQGLVD 116

Query: 371 VHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEY 550
            H  T  C  K     +  ++  K       T + P  +  CE   ++      +F YE 
Sbjct: 117 SHVFTDCCKMKTAARLRCFLSYKKDDADSHDTPLIPRPELTCEVAAENNVSLKERFSYEI 176

Query: 551 SINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERLQ--LKHLSLLTIMSNRLCS 724
           S  +       ++S +  Y + V SCC   + T C L+ +L+   K++  ++   + LC 
Sbjct: 177 SRRHPFLYGPTILSMSACYDTAVQSCCQEENETEC-LRTKLEPIRKYIRGISARHHHLCE 235

Query: 725 QYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMA--KELP 898
               + +  +    LI L +K P AN  ++  L  DV  +   CC+  +  C    ++L 
Sbjct: 236 IGTKFNEKVASAVELILLTKKQPKANFSEIAKLTMDVKNLHETCCEGNTLVCALGRRQLM 295

Query: 899 EYTVKICDNLSSKNSKFTDCCQEKHPW 979
            YT   C N +  +SKF +CC++  P+
Sbjct: 296 NYT---CSNQAILSSKFAECCEQPEPF 319


>ref|XP_979506.2| PREDICTED: alpha-fetoprotein, partial [Mus musculus].
          Length = 363

 Score = 79.7 bits (195), Expect = 3e-15
 Identities = 58/267 (21%), Positives = 111/267 (41%), Gaps = 6/267 (2%)
 Frame = +2

Query: 197 VLYSRKFPSGTFEQISHLVNEVVSLTEAC--CAEGADPDCYDNRTSALSDKSCESDSPFP 370
           ++ ++      ++Q+  +  E++ L E C      +  +C     +A     C +     
Sbjct: 57  IMVAQFLQDAAYDQVQTIAKELLKLAEKCKRLKPESPSECAHQLVAAFLIHICNNQGLVD 116

Query: 371 VHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEY 550
            H  T  C  K     +  ++  K       T + P  +  CE   ++      +F YE 
Sbjct: 117 SHVFTDCCKMKTAARLRCFLSYKKDDADSHDTPLIPRPELTCEVAAENNVSLKERFSYEI 176

Query: 551 SINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERLQ--LKHLSLLTIMSNRLCS 724
           S  +       ++S +  Y + V SCC   + T C L+ +L+   K++  ++   + LC 
Sbjct: 177 SRRHPFLYGPTILSMSACYDTAVQSCCQEENETEC-LRTKLEPIRKYIRGISARHHHLCE 235

Query: 725 QYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMA--KELP 898
               + +  +    LI L +K P AN  ++  L  DV  +   CC+  +  C    ++L 
Sbjct: 236 IGTKFNEKVASAVELILLTKKQPKANFSEIAKLTMDVKNLHETCCEGNTLVCALGRRQLM 295

Query: 899 EYTVKICDNLSSKNSKFTDCCQEKHPW 979
            YT   C N +  +SKF +CC++  P+
Sbjct: 296 NYT---CSNQAILSSKFAECCEQPEPF 319


  Database: RefSeq49_MP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 15,617,559
  Number of sequences in database:  30,036
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 33,483,083
Number of extensions: 861280
Number of successful extensions: 2596
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 2565
Number of HSP's successfully gapped: 14
Length of query: 335
Length of database: 15,617,559
Length adjustment: 102
Effective length of query: 233
Effective length of database: 12,553,887
Effective search space: 2925055671
Effective search space used: 2925055671
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008177
         (1006 letters)

Database: RefSeq49_HP.fasta 
           32,964 sequences; 18,297,164 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|NP_001191236.1| vitamin D-binding protein isoform 3 precurso...   568   e-164
Alignment   gi|NP_001191235.1| vitamin D-binding protein isoform 1 precurso...   554   e-160
Alignment   gi|NP_000574.2| vitamin D-binding protein isoform 1 precursor [...   554   e-160
Alignment   gi|NP_000468.1| serum albumin preproprotein [Homo sapiens].          124   2e-28
Alignment   gi|NP_001124.1| afamin precursor [Homo sapiens].                     100   2e-21
Alignment   gi|NP_001125.1| alpha-fetoprotein precursor [Homo sapiens].           98   1e-20

>ref|NP_001191236.1| vitamin D-binding protein isoform 3 precursor [Homo sapiens].
          Length = 493

 Score =  568 bits (1463), Expect(2) = e-164
 Identities = 272/316 (86%), Positives = 296/316 (93%)
 Frame = +2

Query: 23  QETGGTAGLASRKMKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVL 202
           +E GG A L+ RKMKR+L+ LLAVAF HALERGRDYEK+KVCKE + LGK+DFTSLS+VL
Sbjct: 7   EERGGAARLSGRKMKRVLVLLLAVAFGHALERGRDYEKNKVCKEFSHLGKEDFTSLSLVL 66

Query: 203 YSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPG 382
           YSRKFPSGTFEQ+S LV EVVSLTEACCAEGADPDCYD RTSALS KSCES+SPFPVHPG
Sbjct: 67  YSRKFPSGTFEQVSQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPG 126

Query: 383 TAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINY 562
           TAECCTKEGLE+KLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPK +ANQFM+EYS NY
Sbjct: 127 TAECCTKEGLERKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNY 186

Query: 563 GQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYG 742
           GQAPL+LLVSYTKSYLSMVGSCCTS SPTVCFLKERLQLKHLSLLT +SNR+CSQYAAYG
Sbjct: 187 GQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYG 246

Query: 743 KDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICD 922
           + KSRLS+LIKLAQKVPTA+LEDVLPLAED+  ILSKCC+SASEDCMAKELPE+TVK+CD
Sbjct: 247 EKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCD 306

Query: 923 NLSSKNSKFTDCCQEK 970
           NLS+KNSKF DCCQEK
Sbjct: 307 NLSTKNSKFEDCCQEK 322



 Score = 29.3 bits (64), Expect(2) = e-164
 Identities = 10/13 (76%), Positives = 12/13 (92%)
 Frame = +1

Query: 967  KTPMDIFICTYFM 1005
            KT MD+F+CTYFM
Sbjct: 322  KTAMDVFVCTYFM 334



 Score = 69.3 bits (168), Expect = 5e-12
 Identities = 65/267 (24%), Positives = 111/267 (41%), Gaps = 4/267 (1%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           ++VC + A+ G+      +++  ++K P+   E +  L  ++ ++   CC E A  DC  
Sbjct: 236 NRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCC-ESASEDCMA 294

Query: 317 NRTSALSDKSCESDSPFPVHPGTAECCT-KEGLEKKLCMAALKH-QPQEFPTYVEPTNDE 490
                 + K C++ S    +    +CC  K  ++  +C   +   Q  E P    PTN +
Sbjct: 295 KELPEHTVKLCDNLST--KNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKD 352

Query: 491 ICEAFRKDPKGFANQFMYEYSINYG-QAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LK 664
           +C     DP        Y + ++     P   L    +  L  +G CC     T CF  K
Sbjct: 353 VC-----DPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAK 407

Query: 665 ERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATI 844
             L  K LS        LC+ Y+    + +   +  KLA+++  A L D  P   ++A +
Sbjct: 408 GPLLKKELSSFIDKGQELCADYS----ENTFTEYKKKLAERL-KAKLPDATP--TELAKL 460

Query: 845 LSKCCDSASEDCMAKELPEYTVKICDN 925
           ++K  D AS  C     P Y    CD+
Sbjct: 461 VNKHSDFASNCCSINSPPLY----CDS 483


>ref|NP_001191235.1| vitamin D-binding protein isoform 1 precursor [Homo sapiens].
          Length = 474

 Score =  554 bits (1428), Expect(2) = e-160
 Identities = 265/303 (87%), Positives = 287/303 (94%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 241
           MKR+L+ LLAVAF HALERGRDYEK+KVCKE + LGK+DFTSLS+VLYSRKFPSGTFEQ+
Sbjct: 1   MKRVLVLLLAVAFGHALERGRDYEKNKVCKEFSHLGKEDFTSLSLVLYSRKFPSGTFEQV 60

Query: 242 SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 421
           S LV EVVSLTEACCAEGADPDCYD RTSALS KSCES+SPFPVHPGTAECCTKEGLE+K
Sbjct: 61  SQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPGTAECCTKEGLERK 120

Query: 422 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 601
           LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPK +ANQFM+EYS NYGQAPL+LLVSYTK
Sbjct: 121 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTK 180

Query: 602 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           SYLSMVGSCCTS SPTVCFLKERLQLKHLSLLT +SNR+CSQYAAYG+ KSRLS+LIKLA
Sbjct: 181 SYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLA 240

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QKVPTA+LEDVLPLAED+  ILSKCC+SASEDCMAKELPE+TVK+CDNLS+KNSKF DCC
Sbjct: 241 QKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCC 300

Query: 962 QEK 970
           QEK
Sbjct: 301 QEK 303



 Score = 29.3 bits (64), Expect(2) = e-160
 Identities = 10/13 (76%), Positives = 12/13 (92%)
 Frame = +1

Query: 967  KTPMDIFICTYFM 1005
            KT MD+F+CTYFM
Sbjct: 303  KTAMDVFVCTYFM 315



 Score = 69.3 bits (168), Expect = 5e-12
 Identities = 65/267 (24%), Positives = 111/267 (41%), Gaps = 4/267 (1%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           ++VC + A+ G+      +++  ++K P+   E +  L  ++ ++   CC E A  DC  
Sbjct: 217 NRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCC-ESASEDCMA 275

Query: 317 NRTSALSDKSCESDSPFPVHPGTAECCT-KEGLEKKLCMAALKH-QPQEFPTYVEPTNDE 490
                 + K C++ S    +    +CC  K  ++  +C   +   Q  E P    PTN +
Sbjct: 276 KELPEHTVKLCDNLST--KNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKD 333

Query: 491 ICEAFRKDPKGFANQFMYEYSINYG-QAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LK 664
           +C     DP        Y + ++     P   L    +  L  +G CC     T CF  K
Sbjct: 334 VC-----DPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAK 388

Query: 665 ERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATI 844
             L  K LS        LC+ Y+    + +   +  KLA+++  A L D  P   ++A +
Sbjct: 389 GPLLKKELSSFIDKGQELCADYS----ENTFTEYKKKLAERL-KAKLPDATP--TELAKL 441

Query: 845 LSKCCDSASEDCMAKELPEYTVKICDN 925
           ++K  D AS  C     P Y    CD+
Sbjct: 442 VNKHSDFASNCCSINSPPLY----CDS 464


>ref|NP_000574.2| vitamin D-binding protein isoform 1 precursor [Homo sapiens].
          Length = 474

 Score =  554 bits (1428), Expect(2) = e-160
 Identities = 265/303 (87%), Positives = 287/303 (94%)
 Frame = +2

Query: 62  MKRILIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQI 241
           MKR+L+ LLAVAF HALERGRDYEK+KVCKE + LGK+DFTSLS+VLYSRKFPSGTFEQ+
Sbjct: 1   MKRVLVLLLAVAFGHALERGRDYEKNKVCKEFSHLGKEDFTSLSLVLYSRKFPSGTFEQV 60

Query: 242 SHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKK 421
           S LV EVVSLTEACCAEGADPDCYD RTSALS KSCES+SPFPVHPGTAECCTKEGLE+K
Sbjct: 61  SQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPGTAECCTKEGLERK 120

Query: 422 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTK 601
           LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPK +ANQFM+EYS NYGQAPL+LLVSYTK
Sbjct: 121 LCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTK 180

Query: 602 SYLSMVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLA 781
           SYLSMVGSCCTS SPTVCFLKERLQLKHLSLLT +SNR+CSQYAAYG+ KSRLS+LIKLA
Sbjct: 181 SYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLA 240

Query: 782 QKVPTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCC 961
           QKVPTA+LEDVLPLAED+  ILSKCC+SASEDCMAKELPE+TVK+CDNLS+KNSKF DCC
Sbjct: 241 QKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCC 300

Query: 962 QEK 970
           QEK
Sbjct: 301 QEK 303



 Score = 29.3 bits (64), Expect(2) = e-160
 Identities = 10/13 (76%), Positives = 12/13 (92%)
 Frame = +1

Query: 967  KTPMDIFICTYFM 1005
            KT MD+F+CTYFM
Sbjct: 303  KTAMDVFVCTYFM 315



 Score = 69.3 bits (168), Expect = 5e-12
 Identities = 65/267 (24%), Positives = 111/267 (41%), Gaps = 4/267 (1%)
 Frame = +2

Query: 137 DKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYD 316
           ++VC + A+ G+      +++  ++K P+   E +  L  ++ ++   CC E A  DC  
Sbjct: 217 NRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCC-ESASEDCMA 275

Query: 317 NRTSALSDKSCESDSPFPVHPGTAECCT-KEGLEKKLCMAALKH-QPQEFPTYVEPTNDE 490
                 + K C++ S    +    +CC  K  ++  +C   +   Q  E P    PTN +
Sbjct: 276 KELPEHTVKLCDNLST--KNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKD 333

Query: 491 ICEAFRKDPKGFANQFMYEYSINYG-QAPLTLLVSYTKSYLSMVGSCCTSPSPTVCF-LK 664
           +C     DP        Y + ++     P   L    +  L  +G CC     T CF  K
Sbjct: 334 VC-----DPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAK 388

Query: 665 ERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATI 844
             L  K LS        LC+ Y+    + +   +  KLA+++  A L D  P   ++A +
Sbjct: 389 GPLLKKELSSFIDKGQELCADYS----ENTFTEYKKKLAERL-KAKLPDATP--TELAKL 441

Query: 845 LSKCCDSASEDCMAKELPEYTVKICDN 925
           ++K  D AS  C     P Y    CD+
Sbjct: 442 VNKHSDFASNCCSINSPPLY----CDS 464


>ref|NP_000468.1| serum albumin preproprotein [Homo sapiens].
          Length = 609

 Score =  124 bits (310), Expect = 2e-28
 Identities = 74/299 (24%), Positives = 136/299 (45%), Gaps = 1/299 (0%)
 Frame = +2

Query: 74  LIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLV 253
           L+FL + A+   + R RD  K +V      LG+++F +L ++ +++      FE    LV
Sbjct: 9   LLFLFSSAYSRGVFR-RDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLV 67

Query: 254 NEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMA 433
           NEV    + C A+ +  +C  +  +   DK C   +    +   A+CC K+  E+  C  
Sbjct: 68  NEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFL 127

Query: 434 ALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLS 613
             K      P  V P  D +C AF  + + F  +++YE +  +       L+ + K Y +
Sbjct: 128 QHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKA 187

Query: 614 MVGSCCTSPSPTVCFLKERLQLKHLSLLTIMSNRL-CSQYAAYGKDKSRLSHLIKLAQKV 790
               CC +     C L +  +L+     +    RL C+    +G+   +   + +L+Q+ 
Sbjct: 188 AFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRF 247

Query: 791 PTANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTVKICDNLSSKNSKFTDCCQE 967
           P A   +V  L  D+  + ++CC     +C A +  +    IC+N  S +SK  +CC++
Sbjct: 248 PKAEFAEVSKLVTDLTKVHTECCHGDLLEC-ADDRADLAKYICENQDSISSKLKECCEK 305



 Score = 95.9 bits (237), Expect = 5e-20
 Identities = 68/286 (23%), Positives = 132/286 (46%), Gaps = 12/286 (4%)
 Frame = +2

Query: 146  CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
            C  L   G+  F + ++   S++FP   F ++S LV ++  +   CC  G   +C D+R 
Sbjct: 224  CASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECC-HGDLLECADDRA 282

Query: 326  SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQ--PQEFPT----YVEPTND 487
              L+   CE+     +     ECC K  LEK  C+A +++   P + P+    +VE  + 
Sbjct: 283  D-LAKYICENQDS--ISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVE--SK 337

Query: 488  EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
            ++C+ + +    F   F+YEY+  +    + LL+   K+Y + +  CC +  P  C+ K 
Sbjct: 338  DVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKV 397

Query: 668  RLQLKHL-SLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATI 844
              + K L      +  + C  +   G+ K + + L++  +KVP  +   ++ ++ ++  +
Sbjct: 398  FDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKV 457

Query: 845  LSKCC---DSASEDCMAKELPEYTVKIC--DNLSSKNSKFTDCCQE 967
             SKCC   ++    C    L     ++C     +  + + T CC E
Sbjct: 458  GSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTE 503



 Score = 54.3 bits (129), Expect = 2e-07
 Identities = 38/184 (20%), Positives = 76/184 (41%), Gaps = 6/184 (3%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCA--EGADPDCYDN 319
           C+    LG+  F +  +V Y++K P  +   +  +   +  +   CC   E     C ++
Sbjct: 416 CELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAED 475

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALK----HQPQEFPTYVEPTND 487
             S + ++ C      PV     +CCT+  + ++ C +AL+    + P+EF       + 
Sbjct: 476 YLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHA 535

Query: 488 EICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
           +IC    K+ +      + E   +  +A    L +    + + V  CC +     CF +E
Sbjct: 536 DICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEE 595

Query: 668 RLQL 679
             +L
Sbjct: 596 GKKL 599


>ref|NP_001124.1| afamin precursor [Homo sapiens].
          Length = 599

 Score =  100 bits (250), Expect = 2e-21
 Identities = 58/271 (21%), Positives = 120/271 (44%), Gaps = 2/271 (0%)
 Frame = +2

Query: 170 KDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRTSALSDKSC 349
           +D+   ++++ +++     TFE++  LV ++V   + C A+   P+C     + L +K C
Sbjct: 40  EDNIEYITIIAFAQYVQEATFEEMEKLVKDMVEYKDRCMADKTLPECSKLPNNVLQEKIC 99

Query: 350 ESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEF-PTYVEPTNDEICEAFRKDPKGF 526
             +   P     + CC+K   +++LC    K     F P +     +E C+A+  + +  
Sbjct: 100 AMEG-LPQKHNFSHCCSKVDAQRRLCFFYNKKSDVGFLPPFPTLDPEEKCQAYESNRESL 158

Query: 527 ANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKERLQL-KHLSLLTI 703
            N F+YE +          L++    +  +  SCC   +   C     + + ++L   + 
Sbjct: 159 LNHFLYEVARRNPFVFAPTLLTVAVHFEEVAKSCCEEQNKVNCLQTRAIPVTQYLKAFSS 218

Query: 704 MSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVATILSKCCDSASEDCM 883
               +C     +G       ++  L+QK P    ++++ L EDV++    CC+     C+
Sbjct: 219 YQKHVCGALLKFGTKVVHFIYIAILSQKFPKIEFKELISLVEDVSSNYDGCCEGDVVQCI 278

Query: 884 AKELPEYTVKICDNLSSKNSKFTDCCQEKHP 976
            ++  +    IC    S +SK  +CC++K P
Sbjct: 279 -RDTSKVMNHICSKQDSISSKIKECCEKKIP 308



 Score = 86.7 bits (213), Expect = 3e-17
 Identities = 67/300 (22%), Positives = 129/300 (43%), Gaps = 8/300 (2%)
 Frame = +2

Query: 89   AVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVS 268
            A+     L+    Y+K  VC  L   G      + + + S+KFP   F+++  LV +V S
Sbjct: 206  AIPVTQYLKAFSSYQKH-VCGALLKFGTKVVHFIYIAILSQKFPKIEFKELISLVEDVSS 264

Query: 269  LTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCM--AALK 442
              + CC EG    C  + +  ++    + DS   +     ECC K+  E+  C+  +   
Sbjct: 265  NYDGCC-EGDVVQCIRDTSKVMNHICSKQDS---ISSKIKECCEKKIPERGQCIINSNKD 320

Query: 443  HQPQEFPTYVEPTNDE--ICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSM 616
             +P++         D   +C+    DP  F  +F +EYS  +    +  L+   + Y  +
Sbjct: 321  DRPKDLSLREGKFTDSENVCQERDADPDTFFAKFTFEYSRRHPDLSIPELLRIVQIYKDL 380

Query: 617  VGSCCTSPSPTVCFLKERLQLKHLSLLTI-MSNRLCSQYAAYGKDKSRLSHLIKLAQKVP 793
            + +CC + +P  C+     +    +  ++ M  + C  +   GKD  +  +LI+L +  P
Sbjct: 381  LRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQNLGKDGLKYHYLIRLTKIAP 440

Query: 794  TANLEDVLPLAEDVATILSKCCDSASEDCMAKELPEYTV-KICDNLSSK--NSKFTDCCQ 964
              + E+++ L E + T  + CC  + E      L +    ++C    ++  N     CC+
Sbjct: 441  QLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRTINPAVDHCCK 500



 Score = 58.2 bits (139), Expect = 1e-08
 Identities = 37/178 (20%), Positives = 77/178 (43%), Gaps = 4/178 (2%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
           CK   +LGKD      ++  ++  P  + E++  L  ++V+    CC    +  C DN  
Sbjct: 416 CKHFQNLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLA 475

Query: 326 SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALK----HQPQEFPTYVEPTNDEI 493
             +  + C  +    ++P    CC      ++ C  +LK    + P  F   +   + ++
Sbjct: 476 DLVFGELCGVNENRTINPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADM 535

Query: 494 CEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLKE 667
           C++  ++ +   ++F+        +     L S   ++ ++V  CC + SP VCF +E
Sbjct: 536 CQSQNEELQRKTDRFLVNLVKLKHELTDEELQSLFTNFANVVDKCCKAESPEVCFNEE 593


>ref|NP_001125.1| alpha-fetoprotein precursor [Homo sapiens].
          Length = 609

 Score = 97.8 bits (242), Expect = 1e-20
 Identities = 66/301 (21%), Positives = 130/301 (43%), Gaps = 4/301 (1%)
 Frame = +2

Query: 74  LIFLLAVAFVHALERGRDYEKDKVCKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLV 253
           LIFLL       L R  +Y    +        +     L+ + +++     T++++S +V
Sbjct: 9   LIFLLNFTESRTLHRN-EYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMV 67

Query: 254 NEVVSLTEACCAEGADPDCYDNRTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLC-M 430
            + ++  E    +     C +N+  A  ++ C        + G ++CC++    +  C +
Sbjct: 68  KDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKY-GHSDCCSQSEEGRHNCFL 126

Query: 431 AALKHQPQEFPTYVEPTNDEICEAFRKDPKGFANQFMYEYSINYGQAPLTLLVSYTKSYL 610
           A  K  P   P +  P     CEA+ +D + F N+F+YE +  +       ++ +   Y 
Sbjct: 127 AHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYD 186

Query: 611 SMVGSCCTSPSPTVCF-LKERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQK 787
            ++ SCC + +   CF  K     K L   ++++   C+    +G    +   + KL+QK
Sbjct: 187 KIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQK 246

Query: 788 VPTANLEDVLPLAEDVATILSKCCDSASEDCM--AKELPEYTVKICDNLSSKNSKFTDCC 961
               N  ++  L  DVA +   CC     DC+   +++  Y   IC    + ++K T+CC
Sbjct: 247 FTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSY---ICSQQDTLSNKITECC 303

Query: 962 Q 964
           +
Sbjct: 304 K 304



 Score = 72.0 bits (175), Expect = 8e-13
 Identities = 57/255 (22%), Positives = 108/255 (42%), Gaps = 8/255 (3%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADPDCYDNRT 325
           C  + + G   F ++++   S+KF    F +I  LV +V  + E CC  G   DC  +  
Sbjct: 224 CAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCC-RGDVLDCLQDGE 282

Query: 326 SALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPT-----NDE 490
             +S    + D+   +     ECC    LE+  C+  +  +  E P  + P       D 
Sbjct: 283 KIMSYICSQQDT---LSNKITECCKLTTLERGQCI--IHAENDEKPEGLSPNLNRFLGDR 337

Query: 491 ICEAFRKDPKG-FANQFMYEYSINYGQAPLTLLVSYTKSYLSMVGSCCTSPSPTVCFLK- 664
               F    K  F   F++EYS  + Q  +++++   K Y  ++  C  + +P  C  K 
Sbjct: 338 DFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKG 397

Query: 665 -ERLQLKHLSLLTIMSNRLCSQYAAYGKDKSRLSHLIKLAQKVPTANLEDVLPLAEDVAT 841
            E LQ K++     ++ R C  +   G+   + + L+   +K P     +++ +   +A 
Sbjct: 398 EEELQ-KYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAA 456

Query: 842 ILSKCCDSASEDCMA 886
             + CC  + +  +A
Sbjct: 457 TAATCCQLSEDKLLA 471



 Score = 54.7 bits (130), Expect = 1e-07
 Identities = 45/188 (23%), Positives = 73/188 (38%), Gaps = 10/188 (5%)
 Frame = +2

Query: 146 CKELASLGKDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTEACCAEGADP--DCYDN 319
           C     LG+    +  +V Y++K P  T  ++  +  ++ +    CC    D    C + 
Sbjct: 416 CGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEG 475

Query: 320 RTSALSDKSCESDSPFPVHPGTAECCTKEGLEKKLCMAALKHQPQEFPTYVEPTNDEICE 499
               +    C      PV+PG  +CCT     ++ C ++L        TYV P   +   
Sbjct: 476 AADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVD----ETYVPPAFSDDKF 531

Query: 500 AFRKD---PKGFANQFM-YEYSINY----GQAPLTLLVSYTKSYLSMVGSCCTSPSPTVC 655
            F KD    +G A Q M  E+ IN      Q     L +    +  ++  CC      VC
Sbjct: 532 IFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVC 591

Query: 656 FLKERLQL 679
           F +E  +L
Sbjct: 592 FAEEGQKL 599


  Database: RefSeq49_HP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 18,297,164
  Number of sequences in database:  32,964
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 38,995,332
Number of extensions: 998305
Number of successful extensions: 2949
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 2915
Number of HSP's successfully gapped: 18
Length of query: 335
Length of database: 18,297,164
Length adjustment: 103
Effective length of query: 232
Effective length of database: 14,901,872
Effective search space: 3457234304
Effective search space used: 3457234304
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

BLASTN 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008177
         (1006 letters)

Database: Sscrofa_10.2.fasta 
           4582 sequences; 2,808,509,378 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Sscrofa_Chr08                                                         422   e-115

>Sscrofa_Chr08 
||          Length = 148491826

 Score =  422 bits (213), Expect = e-115
 Identities = 213/213 (100%)
 Strand = Plus / Minus

                                                                            
Query: 322      gacttcagcactgtcagataagtcctgtgaaagcgactccccattcccagtacacccagg 381
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72248243 gacttcagcactgtcagataagtcctgtgaaagcgactccccattcccagtacacccagg 72248184

                                                                            
Query: 382      aactgctgagtgctgcaccaaagagggccttgaaaagaaactctgtatggctgccctgaa 441
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72248183 aactgctgagtgctgcaccaaagagggccttgaaaagaaactctgtatggctgccctgaa 72248124

                                                                            
Query: 442      gcatcagccccaagaattccctacctacgtggagccaacaaatgacgagatctgtgaggc 501
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72248123 gcatcagccccaagaattccctacctacgtggagccaacaaatgacgagatctgtgaggc 72248064

                                                 
Query: 502      attcaggaaagaccccaagggctttgccaatca 534
                |||||||||||||||||||||||||||||||||
Sbjct: 72248063 attcaggaaagaccccaagggctttgccaatca 72248031



 Score =  266 bits (134), Expect = 2e-68
 Identities = 134/134 (100%)
 Strand = Plus / Minus

                                                                            
Query: 759      tcagccatctcataaaattagcccaaaaagtgcctactgcgaatttggaggatgttttgc 818
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72242447 tcagccatctcataaaattagcccaaaaagtgcctactgcgaatttggaggatgttttgc 72242388

                                                                            
Query: 819      cactggctgaagatgttgccacaatcctctccaaatgctgtgactctgcctctgaggact 878
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72242387 cactggctgaagatgttgccacaatcctctccaaatgctgtgactctgcctctgaggact 72242328

                              
Query: 879      gcatggccaaggag 892
                ||||||||||||||
Sbjct: 72242327 gcatggccaaggag 72242314



 Score =  264 bits (133), Expect = 8e-68
 Identities = 133/133 (100%)
 Strand = Plus / Minus

                                                                            
Query: 535      atttatgtatgaatattccattaattatggacaagctcctctgacacttttagtcagtta 594
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72246846 atttatgtatgaatattccattaattatggacaagctcctctgacacttttagtcagtta 72246787

                                                                            
Query: 595      caccaagagttacctctctatggtagggtcctgctgtacttcaccaagcccaacagtatg 654
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72246786 caccaagagttacctctctatggtagggtcctgctgtacttcaccaagcccaacagtatg 72246727

                             
Query: 655      ctttttaaaagag 667
                |||||||||||||
Sbjct: 72246726 ctttttaaaagag 72246714



 Score =  264 bits (133), Expect = 8e-68
 Identities = 133/133 (100%)
 Strand = Plus / Minus

                                                                            
Query: 190      gtcgatggtcctatacagcaggaaatttcccagtggcacctttgaacagatcagtcatct 249
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72250522 gtcgatggtcctatacagcaggaaatttcccagtggcacctttgaacagatcagtcatct 72250463

                                                                            
Query: 250      tgtgaatgaagttgtgtccttgacagaagcctgctgtgctgagggggccgaccctgactg 309
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72250462 tgtgaatgaagttgtgtccttgacagaagcctgctgtgctgagggggccgaccctgactg 72250403

                             
Query: 310      ctatgacaacagg 322
                |||||||||||||
Sbjct: 72250402 ctatgacaacagg 72250390



 Score =  222 bits (112), Expect = 3e-55
 Identities = 112/112 (100%)
 Strand = Plus / Minus

                                                                            
Query: 10       actctgtaatctacaggagacagggggtactgcaggacttgccagcagaaaaatgaagag 69
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72261723 actctgtaatctacaggagacagggggtactgcaggacttgccagcagaaaaatgaagag 72261664

                                                                    
Query: 70       gattctgattttcctgcttgctgtagcatttgtgcatgctttagaaagaggt 121
                ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72261663 gattctgattttcctgcttgctgtagcatttgtgcatgctttagaaagaggt 72261612



 Score =  216 bits (109), Expect = 2e-53
 Identities = 116/117 (99%), Gaps = 1/117 (0%)
 Strand = Plus / Minus

                                                                            
Query: 891      agctgcccgaatacacagtaaaaatctgtgacaacttatcctcaaagaactctaagttta 950
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72241284 agctgcccgaatacacagtaaaaatctgtgacaacttatcctcaaagaactctaagttta 72241225

                                                                         
Query: 951      cggactgttgtcaaga-aaaacacccatggacatttttatatgcacttacttcatgc 1006
                |||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct: 72241224 cggactgttgtcaagaaaaaacacccatggacatttttatatgcacttacttcatgc 72241168



 Score =  192 bits (97), Expect = 3e-46
 Identities = 97/97 (100%)
 Strand = Plus / Minus

                                                                            
Query: 666      agagacttcagcttaaacatctatcacttctcaccattatgtccaatagactctgttcac 725
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72246119 agagacttcagcttaaacatctatcacttctcaccattatgtccaatagactctgttcac 72246060

                                                     
Query: 726      aatatgctgcttatgggaaggataaatcaaggctcag 762
                |||||||||||||||||||||||||||||||||||||
Sbjct: 72246059 aatatgctgcttatgggaaggataaatcaaggctcag 72246023



 Score =  147 bits (74), Expect = 1e-32
 Identities = 74/74 (100%)
 Strand = Plus / Minus

                                                                            
Query: 118      aggtcgagattatgagaaggataaagtctgcaaggaacttgccagtctgggaaaagatga 177
                ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 72251156 aggtcgagattatgagaaggataaagtctgcaaggaacttgccagtctgggaaaagatga 72251097

                              
Query: 178      cttcacatcgttgt 191
                ||||||||||||||
Sbjct: 72251096 cttcacatcgttgt 72251083


  Database: Sscrofa_10.2.fasta
    Posted date:  Nov 16, 2011 10:34 AM
  Number of letters in database: 2,808,509,378
  Number of sequences in database:  4582
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 33,404,746
Number of extensions: 167
Number of successful extensions: 167
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 166
Number of HSP's successfully gapped: 8
Length of query: 1006
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 985
Effective length of database: 2,808,413,156
Effective search space: 2766286958660
Effective search space used: 2766286958660
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 29 (58.0 bits)