Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008703
(975 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001152953.1| [Pyruvate dehydrogenase [lipoamide]] kinase ... 560 e-160
Alignment gi|NP_001069321.1| [Pyruvate dehydrogenase [lipoamide]] kinase ... 446 e-125
Alignment gi|NP_001192886.1| [Pyruvate dehydrogenase [lipoamide]] kinase ... 391 e-109
Alignment gi|XP_002685369.1| PREDICTED: pyruvate dehydrogenase kinase, is... 391 e-109
Alignment gi|NP_001095404.1| [Pyruvate dehydrogenase [lipoamide]] kinase ... 389 e-108
Alignment gi|NP_001095353.1| [Pyruvate dehydrogenase [lipoamide]] kinase ... 379 e-105
Alignment gi|NP_001039371.1| [3-methyl-2-oxobutanoate dehydrogenase [lipo... 106 4e-23
>ref|NP_001152953.1| [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2,
mitochondrial isoform 1 [Bos taurus].
Length = 407
Score = 560 bits (1442), Expect = e-160
Identities = 277/284 (97%), Positives = 281/284 (98%)
Frame = +1
Query: 124 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 303
LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK
Sbjct: 7 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 66
Query: 304 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 483
EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV
Sbjct: 67 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 126
Query: 484 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 663
VPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTL+FDG TNPAHPK
Sbjct: 127 VPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLLFDGRTNPAHPK 186
Query: 664 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY 843
HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASN+KQPIHMVYVPSHLY
Sbjct: 187 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNSKQPIHMVYVPSHLY 246
Query: 844 HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
HMLFELFKNAMRATVESHESS+TLP +KVMVALGEEDLSIKMSD
Sbjct: 247 HMLFELFKNAMRATVESHESSVTLPPVKVMVALGEEDLSIKMSD 290
>ref|NP_001069321.1| [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2,
mitochondrial isoform 2 [Bos taurus].
Length = 343
Score = 446 bits (1147), Expect = e-125
Identities = 219/226 (96%), Positives = 223/226 (98%)
Frame = +1
Query: 298 MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN 477
MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN
Sbjct: 1 MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN 60
Query: 478 DVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAH 657
DVVPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTL+FDG TNPAH
Sbjct: 61 DVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLLFDGRTNPAH 120
Query: 658 PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSH 837
PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASN+KQPIHMVYVPSH
Sbjct: 121 PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNSKQPIHMVYVPSH 180
Query: 838 LYHMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
LYHMLFELFKNAMRATVESHESS+TLP +KVMVALGEEDLSIKMSD
Sbjct: 181 LYHMLFELFKNAMRATVESHESSVTLPPVKVMVALGEEDLSIKMSD 226
>ref|NP_001192886.1| [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1,
mitochondrial [Bos taurus].
Length = 438
Score = 391 bits (1005), Expect = e-109
Identities = 184/278 (66%), Positives = 233/278 (83%), Gaps = 2/278 (0%)
Frame = +1
Query: 148 GAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDR 327
G P ++ +++FSPSPLSMKQFLDFGS NACEKTSF FLRQELPVRLANIMKEI+LLPD
Sbjct: 43 GVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDN 102
Query: 328 VLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGV 507
+L TPSVQLVQSWY+QSL +++EF DK ED +T+ FTD ++ IRNRHNDV+PTMA+GV
Sbjct: 103 LLRTPSVQLVQSWYIQSLQELLEFKDKSAEDAKTIYDFTDTVIRIRNRHNDVIPTMAEGV 162
Query: 508 LEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIF--DGSTNPAHPKHIGSID 681
+EYK+++G DPV++QN+QYFLDRFY+SRISIRML+NQH+L+F G + +H KH+GSI+
Sbjct: 163 VEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSLSHRKHVGSIN 222
Query: 682 PNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFEL 861
PNC+V EV+KD Y+ A+ LCD YY+ SP+LE++E+NA + QPI +VYVPSHLYHM+FEL
Sbjct: 223 PNCSVVEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFEL 282
Query: 862 FKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
FKNAMRAT+E H P I+V V LG+EDL++KMSD
Sbjct: 283 FKNAMRATMEHHADKGVYPPIQVHVTLGKEDLTVKMSD 320
>ref|XP_002685369.1| PREDICTED: pyruvate dehydrogenase kinase, isozyme 1 [Bos taurus].
Length = 438
Score = 391 bits (1005), Expect = e-109
Identities = 184/278 (66%), Positives = 233/278 (83%), Gaps = 2/278 (0%)
Frame = +1
Query: 148 GAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDR 327
G P ++ +++FSPSPLSMKQFLDFGS NACEKTSF FLRQELPVRLANIMKEI+LLPD
Sbjct: 43 GVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDN 102
Query: 328 VLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGV 507
+L TPSVQLVQSWY+QSL +++EF DK ED +T+ FTD ++ IRNRHNDV+PTMA+GV
Sbjct: 103 LLRTPSVQLVQSWYIQSLQELLEFKDKSAEDAKTIYDFTDTVIRIRNRHNDVIPTMAEGV 162
Query: 508 LEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIF--DGSTNPAHPKHIGSID 681
+EYK+++G DPV++QN+QYFLDRFY+SRISIRML+NQH+L+F G + +H KH+GSI+
Sbjct: 163 VEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSLSHRKHVGSIN 222
Query: 682 PNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFEL 861
PNC+V EV+KD Y+ A+ LCD YY+ SP+LE++E+NA + QPI +VYVPSHLYHM+FEL
Sbjct: 223 PNCSVVEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFEL 282
Query: 862 FKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
FKNAMRAT+E H P I+V V LG+EDL++KMSD
Sbjct: 283 FKNAMRATMEHHADKGVYPPIQVHVTLGKEDLTVKMSD 320
>ref|NP_001095404.1| [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3,
mitochondrial [Bos taurus].
Length = 415
Score = 389 bits (1000), Expect = e-108
Identities = 181/275 (65%), Positives = 227/275 (82%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLP+ +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPENLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED + L F L+ +RNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNP HPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE +E P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|NP_001095353.1| [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4,
mitochondrial [Bos taurus].
Length = 407
Score = 379 bits (973), Expect = e-105
Identities = 178/274 (64%), Positives = 230/274 (83%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
P+ +EHFS++SPSPLSMKQ LDFGS NACE+TSF FLRQELPVRLANI+KEI +LPDR++
Sbjct: 16 PREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIYILPDRLV 75
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+T SVQLV+SWY+QSL++++EF +K PED + LS F D L+ +RNRH+DV+PTMAQG+LE
Sbjct: 76 NTSSVQLVKSWYMQSLMELVEFHEKSPEDQKALSDFVDTLIKVRNRHHDVIPTMAQGILE 135
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YKDA DPV+NQN+QYFLDRFY++RIS RML+NQH LIF G +P IGSIDPNC+
Sbjct: 136 YKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIF-GDLQTGNPSLIGSIDPNCD 194
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V+ VV+DA++ +K+LCD+YY+ SP+L + ++N QPIH+VYVPSHL+HMLFELFKNA
Sbjct: 195 VAAVVQDAFECSKMLCDQYYLTSPELNLTQVNVKFPGQPIHIVYVPSHLHHMLFELFKNA 254
Query: 874 MRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
MRATVE ++ +L I+V+V LG+EDL+IK+SD
Sbjct: 255 MRATVEHQQNWPSLTPIQVIVVLGKEDLTIKISD 288
>ref|NP_001039371.1| [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase,
mitochondrial precursor [Bos taurus].
Length = 412
Score = 106 bits (264), Expect = 4e-23
Identities = 73/244 (29%), Positives = 124/244 (50%), Gaps = 2/244 (0%)
Frame = +1
Query: 250 SFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLD-KDPEDHR 426
S +L+QELPVR+A+ +K LP + P++ V Y+++ + +F KD D
Sbjct: 90 SARYLQQELPVRIAHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEA 149
Query: 427 TLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRM 606
Q L+ + H DVV +A+G+ E + D+ + ++YFLD+ SR+ IRM
Sbjct: 150 RYCQLVRQLL---DDHKDVVTLLAEGLRESRKYIEDEKL----VRYFLDKTLTSRLGIRM 202
Query: 607 LINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEI 786
L H + + P +G I + ++++ D A+ LC+ Y +P + I
Sbjct: 203 LATHHLALHEDK-----PDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRI--- 254
Query: 787 NASNAKQPIHMVYVPSHLYHMLFELFKNAMRATVESH-ESSLTLPSIKVMVALGEEDLSI 963
N ++P L ++L EL KNAMRAT+ESH ++ +P + + +A + DL I
Sbjct: 255 ---NGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDIDLVI 311
Query: 964 KMSD 975
++SD
Sbjct: 312 RISD 315
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 33,669,253
Number of extensions: 835720
Number of successful extensions: 2770
Number of sequences better than 1.0e-05: 7
Number of HSP's gapped: 2763
Number of HSP's successfully gapped: 7
Length of query: 325
Length of database: 17,681,374
Length adjustment: 102
Effective length of query: 223
Effective length of database: 14,306,398
Effective search space: 3190326754
Effective search space used: 3190326754
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008703
(975 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_548195.2| PREDICTED: similar to pyruvate dehydrogenase ki... 556 e-158
Alignment gi|XP_859771.1| PREDICTED: similar to [Pyruvate dehydrogenase [... 392 e-109
Alignment gi|XP_537984.2| PREDICTED: similar to pyruvate dehydrogenase ki... 392 e-109
Alignment gi|XP_539427.1| PREDICTED: similar to [Pyruvate dehydrogenase [... 377 e-104
Alignment gi|XP_534032.2| PREDICTED: similar to [Pyruvate dehydrogenase [... 357 9e-99
Alignment gi|XP_535965.2| PREDICTED: similar to [Pyruvate dehydrogenase [... 198 6e-51
Alignment gi|XP_536903.1| PREDICTED: similar to [3-methyl-2-oxobutanoate ... 107 2e-23
>ref|XP_548195.2| PREDICTED: similar to pyruvate dehydrogenase kinase, isoenzyme 2
[Canis familiaris].
Length = 458
Score = 556 bits (1432), Expect = e-158
Identities = 279/285 (97%), Positives = 281/285 (98%), Gaps = 1/285 (0%)
Frame = +1
Query: 124 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 303
LLKNASLAGAPKYIEHFSKFSPSPLS+KQFLDFGSSNACEKTSFTFLRQELPVRLANIMK
Sbjct: 57 LLKNASLAGAPKYIEHFSKFSPSPLSIKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 116
Query: 304 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 483
EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV
Sbjct: 117 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 176
Query: 484 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 663
VPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK
Sbjct: 177 VPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 236
Query: 664 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEIN-ASNAKQPIHMVYVPSHL 840
HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEIN ASN+KQPIHMVYVPSHL
Sbjct: 237 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINTASNSKQPIHMVYVPSHL 296
Query: 841 YHMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
YHMLFELFKNAMRATVESHESSL LP IKVMVALGEEDLSIKMSD
Sbjct: 297 YHMLFELFKNAMRATVESHESSLVLPPIKVMVALGEEDLSIKMSD 341
>ref|XP_859771.1| PREDICTED: similar to [Pyruvate dehydrogenase [lipoamide]] kinase
isozyme 3, mitochondrial precursor (Pyruvate
dehydrogenase kinase isoform 3) isoform 2 [Canis
familiaris].
Length = 406
Score = 392 bits (1007), Expect = e-109
Identities = 183/275 (66%), Positives = 228/275 (82%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLPD +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED + L F L+ +RNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNPAHPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE +E P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|XP_537984.2| PREDICTED: similar to pyruvate dehydrogenase kinase, isoenzyme 3
isoform 1 [Canis familiaris].
Length = 415
Score = 392 bits (1007), Expect = e-109
Identities = 183/275 (66%), Positives = 228/275 (82%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLPD +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED + L F L+ +RNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNPAHPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE +E P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|XP_539427.1| PREDICTED: similar to [Pyruvate dehydrogenase [lipoamide]] kinase
isozyme 4, mitochondrial precursor (Pyruvate
dehydrogenase kinase isoform 4) [Canis familiaris].
Length = 412
Score = 377 bits (967), Expect = e-104
Identities = 177/274 (64%), Positives = 231/274 (84%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
P+ +E FS++SPSPLSMKQ LDFG+ NACE+TSF FLRQELPVRLANI+KEI++LPDR++
Sbjct: 21 PREVELFSRYSPSPLSMKQLLDFGTENACERTSFAFLRQELPVRLANILKEIDILPDRLV 80
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+T SVQLV+SWY+QSL+D++EF +K PED + LS FTD L+ +RNRH +VVPTMAQG++E
Sbjct: 81 NTSSVQLVKSWYIQSLMDLVEFHEKSPEDQKALSDFTDTLIKVRNRHYNVVPTMAQGIIE 140
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YKD DPV+NQN+QYFLDRFY++RIS RML+NQH LIF S +P HIGSIDP+C+
Sbjct: 141 YKDGCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDS-ETGNPTHIGSIDPSCD 199
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V+ VV+DA++ +K+LCD+YY+ SP+L++ ++N QPIH+VYVPSHL+HMLFELFKNA
Sbjct: 200 VAAVVQDAFECSKMLCDQYYLTSPELKLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNA 259
Query: 874 MRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
MRATVE E+ +L I+V+V LG+EDL+IK+SD
Sbjct: 260 MRATVEHQENWPSLTPIEVIVVLGKEDLTIKISD 293
>ref|XP_534032.2| PREDICTED: similar to [Pyruvate dehydrogenase [lipoamide]] kinase
isozyme 1, mitochondrial precursor (Pyruvate
dehydrogenase kinase isoform 1) [Canis familiaris].
Length = 369
Score = 357 bits (916), Expect = 9e-99
Identities = 168/250 (67%), Positives = 210/250 (84%), Gaps = 2/250 (0%)
Frame = +1
Query: 232 NACEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKD 411
NACEKTSF FLRQELPVRLANIMKEI+LLPD +L TPSVQLVQSWY+QSL +++EF DK
Sbjct: 2 NACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLEFKDKS 61
Query: 412 PEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSR 591
E+ +T+ FTD ++ IRNRHNDV+PTMAQGV+EYK+++G DPV++QN+QYFLDRFY+SR
Sbjct: 62 AEEAKTIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSR 121
Query: 592 ISIRMLINQHTLIF--DGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASP 765
ISIRML+NQH+L+F G +PAH KHIGSI+PNC+V EV+KD Y+ A+ LCD YY+ SP
Sbjct: 122 ISIRMLLNQHSLLFGGKGKGSPAHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYINSP 181
Query: 766 DLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLTLPSIKVMVALG 945
+LE++E+NA + QPI +VYVPSHLYHM+FELFKNAMRAT+E H P I+V + LG
Sbjct: 182 ELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHITLG 241
Query: 946 EEDLSIKMSD 975
EDL++KMSD
Sbjct: 242 NEDLTVKMSD 251
>ref|XP_535965.2| PREDICTED: similar to [Pyruvate dehydrogenase [lipoamide]] kinase
isozyme 1, mitochondrial precursor (Pyruvate
dehydrogenase kinase isoform 1) [Canis familiaris].
Length = 323
Score = 198 bits (504), Expect = 6e-51
Identities = 103/215 (47%), Positives = 137/215 (63%), Gaps = 2/215 (0%)
Frame = +1
Query: 337 TPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEY 516
TPSVQLVQSWY+QSL +++EF K E+ +T+ FTD ++ IRNRHNDV+PTMAQGV+EY
Sbjct: 35 TPSVQLVQSWYIQSLHELLEFKGKSAEEAKTIYDFTDTVIQIRNRHNDVIPTMAQGVIEY 94
Query: 517 KDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIF--DGSTNPAHPKHIGSIDPNC 690
K+++G DPV++QN H+L+F G +PAH KHIGS++PNC
Sbjct: 95 KESFGVDPVTSQN---------------------HSLLFGGKGKGSPAHRKHIGSMNPNC 133
Query: 691 NVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKN 870
+V EV+KD Y+ A+ +QPI +VYVPSHLYHM+F LF+N
Sbjct: 134 DVVEVIKDGYENAR-----------------------RQPIQVVYVPSHLYHMVFGLFRN 170
Query: 871 AMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
AMRAT+E H I+V + LG EDL++KMSD
Sbjct: 171 AMRATMEHHADKGVYAPIQVHITLGNEDLTVKMSD 205
>ref|XP_536903.1| PREDICTED: similar to [3-methyl-2-oxobutanoate dehydrogenase
[lipoamide]] kinase, mitochondrial precursor
(Branched-chain alpha-ketoacid dehydrogenase kinase)
(BCKDHKIN) (BCKD-kinase) [Canis familiaris].
Length = 510
Score = 107 bits (266), Expect = 2e-23
Identities = 72/244 (29%), Positives = 127/244 (52%), Gaps = 2/244 (0%)
Frame = +1
Query: 250 SFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEF-LDKDPEDHR 426
S +L+QELPVR+A+ +K LP + P++ V Y+++ + +F L KD D
Sbjct: 188 SARYLQQELPVRIAHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPLIKDQADE- 246
Query: 427 TLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRM 606
+Q+ + + + H DVV +A+G+ E + D+ + +++FLD+ SR+ IRM
Sbjct: 247 --AQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIQDEKL----VRFFLDKTLTSRLGIRM 300
Query: 607 LINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEI 786
L H + + P +G I + ++++ D A+ LC+ Y +P + I
Sbjct: 301 LATHHLALHEDK-----PDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRI--- 352
Query: 787 NASNAKQPIHMVYVPSHLYHMLFELFKNAMRATVESH-ESSLTLPSIKVMVALGEEDLSI 963
N ++P L ++L EL KNAMRAT+ESH ++ +P + + +A + DL I
Sbjct: 353 ---NGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDIDLVI 409
Query: 964 KMSD 975
++SD
Sbjct: 410 RISD 413
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 35,234,649
Number of extensions: 865459
Number of successful extensions: 3000
Number of sequences better than 1.0e-05: 7
Number of HSP's gapped: 2988
Number of HSP's successfully gapped: 7
Length of query: 325
Length of database: 18,874,504
Length adjustment: 103
Effective length of query: 222
Effective length of database: 15,440,896
Effective search space: 3427878912
Effective search space used: 3427878912
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008703
(975 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_003358141.1| PREDICTED: [Pyruvate dehydrogenase [lipoamid... 553 e-158
Alignment gi|XP_003131637.2| PREDICTED: [Pyruvate dehydrogenase [lipoamid... 553 e-158
Alignment gi|NP_001153080.1| [Pyruvate dehydrogenase [lipoamide]] kinase ... 394 e-110
Alignment gi|XP_003360296.1| PREDICTED: [Pyruvate dehydrogenase [lipoamid... 392 e-109
Alignment gi|XP_001927474.1| PREDICTED: [Pyruvate dehydrogenase [lipoamid... 392 e-109
Alignment gi|NP_001152778.1| [Pyruvate dehydrogenase [lipoamide]] kinase ... 379 e-105
Alignment gi|XP_003124519.1| PREDICTED: [3-methyl-2-oxobutanoate dehydrog... 102 2e-22
>ref|XP_003358141.1| PREDICTED: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2,
mitochondrial [Sus scrofa].
Length = 412
Score = 553 bits (1425), Expect = e-158
Identities = 277/284 (97%), Positives = 279/284 (98%)
Frame = +1
Query: 124 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 303
LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK
Sbjct: 7 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 66
Query: 304 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 483
EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDK+PE R +FTDALVTIRNRHNDV
Sbjct: 67 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKEPEPPRACCRFTDALVTIRNRHNDV 126
Query: 484 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 663
VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK
Sbjct: 127 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 186
Query: 664 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY 843
HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY
Sbjct: 187 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY 246
Query: 844 HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD
Sbjct: 247 HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 290
>ref|XP_003131637.2| PREDICTED: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2,
mitochondrial isoform 2 [Sus scrofa].
Length = 407
Score = 553 bits (1425), Expect = e-158
Identities = 277/284 (97%), Positives = 279/284 (98%)
Frame = +1
Query: 124 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 303
LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK
Sbjct: 7 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 66
Query: 304 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 483
EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDK+PE R +FTDALVTIRNRHNDV
Sbjct: 67 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKEPEPPRACCRFTDALVTIRNRHNDV 126
Query: 484 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 663
VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK
Sbjct: 127 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 186
Query: 664 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY 843
HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY
Sbjct: 187 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY 246
Query: 844 HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD
Sbjct: 247 HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 290
>ref|NP_001153080.1| [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1,
mitochondrial [Sus scrofa].
Length = 438
Score = 394 bits (1011), Expect = e-110
Identities = 186/278 (66%), Positives = 232/278 (83%), Gaps = 2/278 (0%)
Frame = +1
Query: 148 GAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDR 327
G P ++ +++FSPSPLSMKQFLDFGS NACEKTSF FLRQELPVRLANIMKEI+LLPD
Sbjct: 43 GVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDN 102
Query: 328 VLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGV 507
+L TPSVQLVQSWY+QSL ++++F DK ED +T+ FTD ++ IRNRHNDV+PTMAQGV
Sbjct: 103 LLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYDFTDTVIRIRNRHNDVIPTMAQGV 162
Query: 508 LEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIF--DGSTNPAHPKHIGSID 681
+EYK+++G DPV++QN+QYFLDRFY+SRISIRML+NQH+L+F G + +H KHIGSI+
Sbjct: 163 VEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSLSHQKHIGSIN 222
Query: 682 PNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFEL 861
PNCNV EV+KD Y+ A+ LCD YY+ SP+LE++E+NA + QPI +VYVPSHLYHM+FEL
Sbjct: 223 PNCNVVEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFEL 282
Query: 862 FKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
FKNAMRAT+E H P I+V V LG EDL++KMSD
Sbjct: 283 FKNAMRATMEHHADKGVYPPIQVHVTLGNEDLTVKMSD 320
>ref|XP_003360296.1| PREDICTED: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3,
mitochondrial isoform 2 [Sus scrofa].
Length = 406
Score = 392 bits (1008), Expect = e-109
Identities = 183/275 (66%), Positives = 228/275 (82%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLPD +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED + L F L+ +RNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNPAHPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE +E P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|XP_001927474.1| PREDICTED: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3,
mitochondrial isoform 1 [Sus scrofa].
Length = 415
Score = 392 bits (1008), Expect = e-109
Identities = 183/275 (66%), Positives = 228/275 (82%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLPD +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED + L F L+ +RNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNPAHPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE +E P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|NP_001152778.1| [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4,
mitochondrial [Sus scrofa].
Length = 407
Score = 379 bits (972), Expect = e-105
Identities = 177/274 (64%), Positives = 232/274 (84%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
P+ +EHFS++SPSPLS+KQ LDFGS NACE+TSF FLRQELPVRLANI+KEI +LPDR++
Sbjct: 16 PREVEHFSRYSPSPLSIKQLLDFGSENACERTSFAFLRQELPVRLANILKEIYILPDRLV 75
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+T SVQLV+SWY+QSL++++EF +K PED + L+ F D L+ +RNRH++VVPTMAQG+LE
Sbjct: 76 NTSSVQLVKSWYIQSLMELVEFHEKSPEDQKVLADFVDTLIKVRNRHHNVVPTMAQGILE 135
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YKDA DPV+NQN+QYFLDRFY++RIS RML+NQH LIF A+P HIGSIDPNC+
Sbjct: 136 YKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIF-SDLQTANPSHIGSIDPNCD 194
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V+ VV+DA++ +K+LC++YY+ SP+L++ ++N QPIH+VYVPSHL+HMLFELFKNA
Sbjct: 195 VAAVVQDAFECSKMLCNQYYLTSPELKLTQVNGKLPGQPIHIVYVPSHLHHMLFELFKNA 254
Query: 874 MRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
MRATVE E+ +L I+V+V LG+EDL+IK+SD
Sbjct: 255 MRATVEHQENWPSLTPIEVIVVLGKEDLTIKISD 288
>ref|XP_003124519.1| PREDICTED: [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]]
kinase, mitochondrial [Sus scrofa].
Length = 412
Score = 102 bits (255), Expect = 2e-22
Identities = 70/244 (28%), Positives = 125/244 (51%), Gaps = 2/244 (0%)
Frame = +1
Query: 250 SFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLD-KDPEDHR 426
S +L+QELPVR+A+ +K+ LP + P++ V Y+++ + +F KD D
Sbjct: 90 SARYLQQELPVRIAHRIKDFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADE- 148
Query: 427 TLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRM 606
+Q+ + + + H DVV +A+G+ E + D+ + ++ FLD+ SR+ IRM
Sbjct: 149 --AQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKL----VRSFLDKTLTSRLGIRM 202
Query: 607 LINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEI 786
L H + + P +G I + ++++ D A+ LC+ Y +P + I
Sbjct: 203 LATHHLALHEDK-----PDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRI--- 254
Query: 787 NASNAKQPIHMVYVPSHLYHMLFELFKNAMRATVESH-ESSLTLPSIKVMVALGEEDLSI 963
N ++P L ++L EL KNAMRAT+E H ++ +P + + +A + DL I
Sbjct: 255 ---NGHVAARFPFIPMPLDYILPELLKNAMRATMECHLDTPYNVPDVVITIANNDIDLVI 311
Query: 964 KMSD 975
++SD
Sbjct: 312 RISD 315
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 21,724,553
Number of extensions: 537867
Number of successful extensions: 1840
Number of sequences better than 1.0e-05: 7
Number of HSP's gapped: 1833
Number of HSP's successfully gapped: 7
Length of query: 325
Length of database: 11,343,932
Length adjustment: 99
Effective length of query: 226
Effective length of database: 8,879,129
Effective search space: 2006683154
Effective search space used: 2006683154
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008703
(975 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_598428.2| pyruvate dehydrogenase kinase, isoenzyme 2 prec... 556 e-159
Alignment gi|NP_766253.2| pyruvate dehydrogenase kinase, isozyme 1 precur... 400 e-112
Alignment gi|NP_663605.1| pyruvate dehydrogenase kinase, isozyme 3 precur... 398 e-111
Alignment gi|NP_038771.1| pyruvate dehydrogenase kinase, isozyme 4 precur... 384 e-107
Alignment gi|NP_033869.1| [3-methyl-2-oxobutanoate dehydrogenase [lipoami... 106 3e-23
>ref|NP_598428.2| pyruvate dehydrogenase kinase, isoenzyme 2 precursor [Mus
musculus].
Length = 407
Score = 556 bits (1434), Expect = e-159
Identities = 275/284 (96%), Positives = 280/284 (98%)
Frame = +1
Query: 124 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 303
LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK
Sbjct: 7 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 66
Query: 304 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 483
EINLLPDRVL TPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV
Sbjct: 67 EINLLPDRVLGTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 126
Query: 484 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 663
VPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK
Sbjct: 127 VPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 186
Query: 664 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY 843
HIGSIDPNC+VS+VVKDAYDMAKLLCDKYYMASPDLEIQE+NA+NA QPIHMVYVPSHLY
Sbjct: 187 HIGSIDPNCSVSDVVKDAYDMAKLLCDKYYMASPDLEIQEVNATNANQPIHMVYVPSHLY 246
Query: 844 HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
HMLFELFKNAMRATVESHESSLTLP IK+MVALGEEDLSIKMSD
Sbjct: 247 HMLFELFKNAMRATVESHESSLTLPPIKIMVALGEEDLSIKMSD 290
>ref|NP_766253.2| pyruvate dehydrogenase kinase, isozyme 1 precursor [Mus musculus].
Length = 434
Score = 400 bits (1029), Expect = e-112
Identities = 188/280 (67%), Positives = 235/280 (83%)
Frame = +1
Query: 136 ASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINL 315
AS G P ++ +++FSPSPLSMKQFLDFGS NACEKTSF FLRQELPVRLANIMKEI+L
Sbjct: 37 ASELGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISL 96
Query: 316 LPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTM 495
LPD +L TPSVQLVQSWY+QSL ++++F DK ED +T+ +FTD ++ IRNRHNDV+PTM
Sbjct: 97 LPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTM 156
Query: 496 AQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGS 675
AQGV EYK+++G DPV++QN+QYFLDRFY+SRISIRML+NQH+L+F G +P+H KHIGS
Sbjct: 157 AQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGS 216
Query: 676 IDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLF 855
I+PNC+V EV+KD Y+ A+ LCD YY+ SP+LE++E+NA + Q I +VYVPSHLYHM+F
Sbjct: 217 INPNCDVVEVIKDGYENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVF 276
Query: 856 ELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
ELFKNAMRAT+E H P I+V V LGEEDL++KMSD
Sbjct: 277 ELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSD 316
>ref|NP_663605.1| pyruvate dehydrogenase kinase, isozyme 3 precursor [Mus musculus].
Length = 415
Score = 398 bits (1022), Expect = e-111
Identities = 186/275 (67%), Positives = 229/275 (83%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLPD +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED R L F + L+ IRNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPRVLDNFLNVLINIRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNPAHPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE HE P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELHEDKKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|NP_038771.1| pyruvate dehydrogenase kinase, isozyme 4 precursor [Mus musculus].
Length = 412
Score = 384 bits (985), Expect = e-107
Identities = 184/283 (65%), Positives = 234/283 (82%)
Frame = +1
Query: 127 LKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKE 306
L +ASL P+ +E FS++SPSPLSMKQ LDFGS NACE+TSF FLRQELPVRLANI+KE
Sbjct: 14 LSSASLV--PREVELFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKE 71
Query: 307 INLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVV 486
I++LPDR+++TPSVQLV+SWY+QSL+D++EF +K PED + LS+F D LV +RNRH++VV
Sbjct: 72 IDILPDRLVNTPSVQLVKSWYIQSLMDLVEFHEKSPEDQKALSEFVDTLVKVRNRHHNVV 131
Query: 487 PTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKH 666
PTMAQG+LEYKD DPV+NQN+QYFLDRFY++RIS RML+NQH LIF S +P H
Sbjct: 132 PTMAQGILEYKDTCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDS-KTGNPSH 190
Query: 667 IGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYH 846
IGSIDPNC+V VV+DA++ AK+LCD+YY+ SP+L + ++N QPIH+VYVPSHL+H
Sbjct: 191 IGSIDPNCDVVAVVQDAFECAKMLCDQYYLTSPELNLTQVNGKFPGQPIHIVYVPSHLHH 250
Query: 847 MLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
MLFELFKNAMRATVE E+ +L ++ V LG+EDL+IK+SD
Sbjct: 251 MLFELFKNAMRATVEHQENRPSLTPVEATVVLGKEDLTIKISD 293
>ref|NP_033869.1| [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase,
mitochondrial precursor [Mus musculus].
Length = 412
Score = 106 bits (264), Expect = 3e-23
Identities = 71/241 (29%), Positives = 125/241 (51%), Gaps = 2/241 (0%)
Frame = +1
Query: 259 FLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLD-KDPEDHRTLS 435
+L+QELPVR+A+ +K LP + P++ V Y+++ + +F KD D +
Sbjct: 93 YLQQELPVRIAHRIKGFRSLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADE---A 149
Query: 436 QFTDALVTIRNRHNDVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLIN 615
Q+ + + + H DVV +A+G+ E + D+ + ++YFLD+ SR+ IRML
Sbjct: 150 QYCQLVRQLLDDHKDVVTLLAEGLRESRKHIQDEKL----VRYFLDKTLTSRLGIRMLAT 205
Query: 616 QHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAS 795
H + + P +G I + ++++ D A+ LC+ Y +P + I
Sbjct: 206 HHLALHEDK-----PDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRI------ 254
Query: 796 NAKQPIHMVYVPSHLYHMLFELFKNAMRATVESH-ESSLTLPSIKVMVALGEEDLSIKMS 972
N ++P L ++L EL KNAMRAT+ESH ++ +P + + +A + DL I++S
Sbjct: 255 NGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDIDLIIRIS 314
Query: 973 D 975
D
Sbjct: 315 D 315
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 29,200,554
Number of extensions: 711865
Number of successful extensions: 2393
Number of sequences better than 1.0e-05: 5
Number of HSP's gapped: 2386
Number of HSP's successfully gapped: 5
Length of query: 325
Length of database: 15,617,559
Length adjustment: 102
Effective length of query: 223
Effective length of database: 12,553,887
Effective search space: 2799516801
Effective search space used: 2799516801
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008703
(975 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_002602.2| pyruvate dehydrogenase kinase, isozyme 2 isofor... 560 e-160
Alignment gi|NP_001186828.1| pyruvate dehydrogenase kinase, isozyme 2 iso... 447 e-126
Alignment gi|NP_001186827.1| pyruvate dehydrogenase kinase, isozyme 2 iso... 447 e-126
Alignment gi|NP_002601.1| pyruvate dehydrogenase kinase, isozyme 1 precur... 397 e-111
Alignment gi|NP_005382.1| pyruvate dehydrogenase kinase, isozyme 3 isofor... 390 e-108
Alignment gi|NP_001135858.1| pyruvate dehydrogenase kinase, isozyme 3 iso... 390 e-108
Alignment gi|NP_002603.1| pyruvate dehydrogenase kinase, isozyme 4 precur... 380 e-105
Alignment gi|NP_001186829.1| pyruvate dehydrogenase kinase, isozyme 2 iso... 332 4e-91
Alignment gi|NP_001116429.1| [3-methyl-2-oxobutanoate dehydrogenase [lipo... 107 2e-23
Alignment gi|NP_005872.2| [3-methyl-2-oxobutanoate dehydrogenase [lipoami... 107 2e-23
>ref|NP_002602.2| pyruvate dehydrogenase kinase, isozyme 2 isoform 1 precursor [Homo
sapiens].
Length = 407
Score = 560 bits (1444), Expect = e-160
Identities = 279/284 (98%), Positives = 281/284 (98%)
Frame = +1
Query: 124 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 303
LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK
Sbjct: 7 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 66
Query: 304 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 483
EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV
Sbjct: 67 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 126
Query: 484 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 663
VPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK
Sbjct: 127 VPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPK 186
Query: 664 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLY 843
HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINA+N+KQPIHMVYVPSHLY
Sbjct: 187 HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLY 246
Query: 844 HMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
HMLFELFKNAMRATVESHESSL LP IKVMVALGEEDLSIKMSD
Sbjct: 247 HMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSD 290
>ref|NP_001186828.1| pyruvate dehydrogenase kinase, isozyme 2 isoform 2 [Homo sapiens].
Length = 343
Score = 447 bits (1149), Expect = e-126
Identities = 221/226 (97%), Positives = 223/226 (98%)
Frame = +1
Query: 298 MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN 477
MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN
Sbjct: 1 MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN 60
Query: 478 DVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAH 657
DVVPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAH
Sbjct: 61 DVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAH 120
Query: 658 PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSH 837
PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINA+N+KQPIHMVYVPSH
Sbjct: 121 PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSH 180
Query: 838 LYHMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
LYHMLFELFKNAMRATVESHESSL LP IKVMVALGEEDLSIKMSD
Sbjct: 181 LYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSD 226
>ref|NP_001186827.1| pyruvate dehydrogenase kinase, isozyme 2 isoform 2 [Homo sapiens].
Length = 343
Score = 447 bits (1149), Expect = e-126
Identities = 221/226 (97%), Positives = 223/226 (98%)
Frame = +1
Query: 298 MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN 477
MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN
Sbjct: 1 MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHN 60
Query: 478 DVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAH 657
DVVPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAH
Sbjct: 61 DVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAH 120
Query: 658 PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSH 837
PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINA+N+KQPIHMVYVPSH
Sbjct: 121 PKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSH 180
Query: 838 LYHMLFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
LYHMLFELFKNAMRATVESHESSL LP IKVMVALGEEDLSIKMSD
Sbjct: 181 LYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSD 226
>ref|NP_002601.1| pyruvate dehydrogenase kinase, isozyme 1 precursor [Homo sapiens].
Length = 436
Score = 397 bits (1020), Expect = e-111
Identities = 188/282 (66%), Positives = 235/282 (83%), Gaps = 2/282 (0%)
Frame = +1
Query: 136 ASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINL 315
AS G P ++ +++FSPSPLSMKQFLDFGS NACEKTSF FLRQELPVRLANIMKEI+L
Sbjct: 37 ASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISL 96
Query: 316 LPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTM 495
LPD +L TPSVQLVQSWY+QSL ++++F DK ED + + FTD ++ IRNRHNDV+PTM
Sbjct: 97 LPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTM 156
Query: 496 AQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIF--DGSTNPAHPKHI 669
AQGV+EYK+++G DPV++QN+QYFLDRFY+SRISIRML+NQH+L+F G +P+H KHI
Sbjct: 157 AQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHI 216
Query: 670 GSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHM 849
GSI+PNCNV EV+KD Y+ A+ LCD YY+ SP+LE++E+NA + QPI +VYVPSHLYHM
Sbjct: 217 GSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHM 276
Query: 850 LFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
+FELFKNAMRAT+E H + P I+V V LG EDL++KMSD
Sbjct: 277 VFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSD 318
>ref|NP_005382.1| pyruvate dehydrogenase kinase, isozyme 3 isoform 2 precursor [Homo
sapiens].
Length = 406
Score = 390 bits (1002), Expect = e-108
Identities = 182/275 (66%), Positives = 227/275 (82%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLPD +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED + L F L+ +RNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNP HPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE +E P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|NP_001135858.1| pyruvate dehydrogenase kinase, isozyme 3 isoform 1 precursor [Homo
sapiens].
Length = 415
Score = 390 bits (1002), Expect = e-108
Identities = 182/275 (66%), Positives = 227/275 (82%), Gaps = 1/275 (0%)
Frame = +1
Query: 154 PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVL 333
PK IE +S+FSPSPLS+KQFLDFG NACEKTS+ FLR+ELPVRLAN M+E+NLLPD +L
Sbjct: 13 PKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLL 72
Query: 334 STPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLE 513
+ PSV LVQSWY+QS L+++E+ +K PED + L F L+ +RNRHNDVVPTMAQGV+E
Sbjct: 73 NRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIE 132
Query: 514 YKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCN 693
YK+ +G DP + NIQYFLDRFY +RIS RMLINQHTL+F G TNP HPKHIGSIDP CN
Sbjct: 133 YKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCN 192
Query: 694 VSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHMLFELFKNA 873
V++VVKDAY+ AK+LC++YY+ +P+LE++E NA +PI +VYVPSHL+HMLFELFKN+
Sbjct: 193 VADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNS 252
Query: 874 MRATVESHESSLT-LPSIKVMVALGEEDLSIKMSD 975
MRATVE +E P++K +V LG+EDLSIK+SD
Sbjct: 253 MRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISD 287
>ref|NP_002603.1| pyruvate dehydrogenase kinase, isozyme 4 precursor [Homo sapiens].
Length = 411
Score = 380 bits (976), Expect = e-105
Identities = 180/282 (63%), Positives = 236/282 (83%), Gaps = 3/282 (1%)
Frame = +1
Query: 139 SLAGA---PKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEI 309
SL GA P+ +EHFS++SPSPLSMKQ LDFGS NACE+TSF FLRQELPVRLANI+KEI
Sbjct: 13 SLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEI 72
Query: 310 NLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVP 489
++LP ++++T SVQLV+SWY+QSL+D++EF +K P+D + LS F D L+ +RNRH++VVP
Sbjct: 73 DILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVP 132
Query: 490 TMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHI 669
TMAQG++EYKDA DPV+NQN+QYFLDRFY++RIS RML+NQH LIF S +P HI
Sbjct: 133 TMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDS-QTGNPSHI 191
Query: 670 GSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINASNAKQPIHMVYVPSHLYHM 849
GSIDPNC+V VV+DA++ +++LCD+YY++SP+L++ ++N QPIH+VYVPSHL+HM
Sbjct: 192 GSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHM 251
Query: 850 LFELFKNAMRATVESHESSLTLPSIKVMVALGEEDLSIKMSD 975
LFELFKNAMRATVE E+ +L I+V+V LG+EDL+IK+SD
Sbjct: 252 LFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISD 293
>ref|NP_001186829.1| pyruvate dehydrogenase kinase, isozyme 2 isoform 3 precursor [Homo
sapiens].
Length = 199
Score = 332 bits (850), Expect = 4e-91
Identities = 165/167 (98%), Positives = 166/167 (99%)
Frame = +1
Query: 124 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 303
LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK
Sbjct: 7 LLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMK 66
Query: 304 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 483
EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV
Sbjct: 67 EINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDV 126
Query: 484 VPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHT 624
VPTMAQGVLEYKD YGDDPVSNQNIQYFLDRFYLSRISIRMLINQH+
Sbjct: 127 VPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHS 173
>ref|NP_001116429.1| [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase,
mitochondrial isoform b [Homo sapiens].
Length = 365
Score = 107 bits (267), Expect = 2e-23
Identities = 72/244 (29%), Positives = 126/244 (51%), Gaps = 2/244 (0%)
Frame = +1
Query: 250 SFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLD-KDPEDHR 426
S +L+QELPVR+A+ +K LP + P++ V Y+++ + +F KD D
Sbjct: 90 SARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADE- 148
Query: 427 TLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRM 606
+Q+ + + + H DVV +A+G+ E + D+ + ++YFLD+ SR+ IRM
Sbjct: 149 --AQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKL----VRYFLDKTLTSRLGIRM 202
Query: 607 LINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEI 786
L H + + P +G I + ++++ D A+ LC+ Y +P + I
Sbjct: 203 LATHHLALHEDK-----PDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRI--- 254
Query: 787 NASNAKQPIHMVYVPSHLYHMLFELFKNAMRATVESH-ESSLTLPSIKVMVALGEEDLSI 963
N ++P L ++L EL KNAMRAT+ESH ++ +P + + +A + DL I
Sbjct: 255 ---NGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLII 311
Query: 964 KMSD 975
++SD
Sbjct: 312 RISD 315
>ref|NP_005872.2| [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase,
mitochondrial isoform a [Homo sapiens].
Length = 412
Score = 107 bits (267), Expect = 2e-23
Identities = 72/244 (29%), Positives = 126/244 (51%), Gaps = 2/244 (0%)
Frame = +1
Query: 250 SFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLD-KDPEDHR 426
S +L+QELPVR+A+ +K LP + P++ V Y+++ + +F KD D
Sbjct: 90 SARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADE- 148
Query: 427 TLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDAYGDDPVSNQNIQYFLDRFYLSRISIRM 606
+Q+ + + + H DVV +A+G+ E + D+ + ++YFLD+ SR+ IRM
Sbjct: 149 --AQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKL----VRYFLDKTLTSRLGIRM 202
Query: 607 LINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEI 786
L H + + P +G I + ++++ D A+ LC+ Y +P + I
Sbjct: 203 LATHHLALHEDK-----PDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRI--- 254
Query: 787 NASNAKQPIHMVYVPSHLYHMLFELFKNAMRATVESH-ESSLTLPSIKVMVALGEEDLSI 963
N ++P L ++L EL KNAMRAT+ESH ++ +P + + +A + DL I
Sbjct: 255 ---NGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLII 311
Query: 964 KMSD 975
++SD
Sbjct: 312 RISD 315
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 34,544,355
Number of extensions: 855600
Number of successful extensions: 2883
Number of sequences better than 1.0e-05: 10
Number of HSP's gapped: 2873
Number of HSP's successfully gapped: 10
Length of query: 325
Length of database: 18,297,164
Length adjustment: 103
Effective length of query: 222
Effective length of database: 14,901,872
Effective search space: 3308215584
Effective search space used: 3308215584
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-008703
(975 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_Chr12 428 e-117
>Sscrofa_Chr12
|| Length = 63588571
Score = 428 bits (216), Expect = e-117
Identities = 222/224 (99%)
Strand = Plus / Plus
Query: 1 ctgggcggcgcgggaccctattgggccaagccgagcgccaacgctgcccgcgcggggtgc 60
|||||||||||||||||| | |||||||||||||||||||||||||||||||||||||||
Sbjct: 26313941 ctgggcggcgcgggacccgagtgggccaagccgagcgccaacgctgcccgcgcggggtgc 26314000
Query: 61 ggagcccgagcccgagcccgagcccgaggcgcggccgccgctgccatgcgctgggtccgc 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26314001 ggagcccgagcccgagcccgagcccgaggcgcggccgccgctgccatgcgctgggtccgc 26314060
Query: 121 gcgctactgaagaatgcgtccctggcaggggcgcccaagtacatcgagcacttcagcaag 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26314061 gcgctactgaagaatgcgtccctggcaggggcgcccaagtacatcgagcacttcagcaag 26314120
Query: 181 ttctccccgtccccgttgtccatgaagcagtttctggacttcgg 224
||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26314121 ttctccccgtccccgttgtccatgaagcagtttctggacttcgg 26314164
Score = 367 bits (185), Expect = 8e-99
Identities = 185/185 (100%)
Strand = Plus / Plus
Query: 438 gttcaccgacgccctggtcaccatccggaaccgccacaatgacgtggtacccaccatggc 497
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26326847 gttcaccgacgccctggtcaccatccggaaccgccacaatgacgtggtacccaccatggc 26326906
Query: 498 gcagggcgtgctggagtacaaggacgcctacggcgacgacccggtctccaaccagaacat 557
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26326907 gcagggcgtgctggagtacaaggacgcctacggcgacgacccggtctccaaccagaacat 26326966
Query: 558 ccagtacttcctggaccgcttctacctcagccgcatctccatccgcatgctcatcaacca 617
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26326967 ccagtacttcctggaccgcttctacctcagccgcatctccatccgcatgctcatcaacca 26327026
Query: 618 gcaca 622
|||||
Sbjct: 26327027 gcaca 26327031
Score = 287 bits (145), Expect = 6e-75
Identities = 145/145 (100%)
Strand = Plus / Plus
Query: 223 ggatccagcaatgcctgtgagaaaacctcgttcaccttcctcaggcaggagctgccagtg 282
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26316106 ggatccagcaatgcctgtgagaaaacctcgttcaccttcctcaggcaggagctgccagtg 26316165
Query: 283 cgcctggccaacatcatgaaagagatcaacctgcttcccgaccgggtgctgagcacaccc 342
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26316166 cgcctggccaacatcatgaaagagatcaacctgcttcccgaccgggtgctgagcacaccc 26316225
Query: 343 tcggtgcagctggtacagagctggt 367
|||||||||||||||||||||||||
Sbjct: 26316226 tcggtgcagctggtacagagctggt 26316250
Score = 200 bits (101), Expect = 1e-48
Identities = 101/101 (100%)
Strand = Plus / Plus
Query: 866 agaacgccatgcgagcaactgtggaaagccatgaatccagcctcactctcccatctatca 925
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26332243 agaacgccatgcgagcaactgtggaaagccatgaatccagcctcactctcccatctatca 26332302
Query: 926 aggtcatggtggccttgggcgaggaagatctttccatcaaa 966
|||||||||||||||||||||||||||||||||||||||||
Sbjct: 26332303 aggtcatggtggccttgggcgaggaagatctttccatcaaa 26332343
Score = 178 bits (90), Expect = 4e-42
Identities = 90/90 (100%)
Strand = Plus / Plus
Query: 623 ccctgatctttgatggcagcacgaacccagcccaccctaaacacattggcagcattgacc 682
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26328613 ccctgatctttgatggcagcacgaacccagcccaccctaaacacattggcagcattgacc 26328672
Query: 683 ccaactgcaacgtctctgaggtggtgaaag 712
||||||||||||||||||||||||||||||
Sbjct: 26328673 ccaactgcaacgtctctgaggtggtgaaag 26328702
Score = 159 bits (80), Expect = 3e-36
Identities = 80/80 (100%)
Strand = Plus / Plus
Query: 711 agatgcctacgacatggccaagcttctgtgtgacaagtattacatggcctcacctgacct 770
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26328904 agatgcctacgacatggccaagcttctgtgtgacaagtattacatggcctcacctgacct 26328963
Query: 771 ggagatccaggagatcaatg 790
||||||||||||||||||||
Sbjct: 26328964 ggagatccaggagatcaatg 26328983
Score = 155 bits (78), Expect = 5e-35
Identities = 78/78 (100%)
Strand = Plus / Plus
Query: 790 gcatccaacgccaaacagccaattcacatggtctatgtcccctcccacctctaccacatg 849
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 26332077 gcatccaacgccaaacagccaattcacatggtctatgtcccctcccacctctaccacatg 26332136
Query: 850 ctgtttgaactcttcaag 867
||||||||||||||||||
Sbjct: 26332137 ctgtttgaactcttcaag 26332154
Score = 93.7 bits (47), Expect = 2e-16
Identities = 50/51 (98%)
Strand = Plus / Plus
Query: 366 gtatgtccagagtctcctggacatcatggagttcctggacaaggaccctga 416
||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct: 26326378 gtatgtccagagtctcctggacatcatggagttcctggacaaggaacctga 26326428
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 22,628,403
Number of extensions: 214
Number of successful extensions: 214
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 212
Number of HSP's successfully gapped: 8
Length of query: 975
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 954
Effective length of database: 2,808,413,156
Effective search space: 2679226150824
Effective search space used: 2679226150824
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 29 (58.0 bits)