Animal-Genome cDNA 20110601C-008978


Search to RefSeqBP_Rel49

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008978
         (917 letters)

Database: RefSeq49_BP.fasta 
           33,088 sequences; 17,681,374 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|NP_001106791.1| immediate early response 3-interacting prote...   160   1e-39
Alignment   gi|XP_002691505.1| PREDICTED: immediate early response 3 intera...   160   1e-39
Alignment   gi|XP_002684312.1| PREDICTED: immediate early response 3 intera...   160   1e-39

>ref|NP_001106791.1| immediate early response 3-interacting protein 1 [Bos taurus].
          Length = 82

 Score =  160 bits (406), Expect = 1e-39
 Identities = 82/82 (100%), Positives = 82/82 (100%)
 Frame = +1

Query: 88  MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 267
           MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR
Sbjct: 1   MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 60

Query: 268 TVMRVPLIIVNSIAIVLLLLFG 333
           TVMRVPLIIVNSIAIVLLLLFG
Sbjct: 61  TVMRVPLIIVNSIAIVLLLLFG 82


>ref|XP_002691505.1| PREDICTED: immediate early response 3 interacting protein 1-like
           [Bos taurus].
          Length = 82

 Score =  160 bits (406), Expect = 1e-39
 Identities = 82/82 (100%), Positives = 82/82 (100%)
 Frame = +1

Query: 88  MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 267
           MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR
Sbjct: 1   MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 60

Query: 268 TVMRVPLIIVNSIAIVLLLLFG 333
           TVMRVPLIIVNSIAIVLLLLFG
Sbjct: 61  TVMRVPLIIVNSIAIVLLLLFG 82


>ref|XP_002684312.1| PREDICTED: immediate early response 3 interacting protein 1 [Bos
           taurus].
          Length = 82

 Score =  160 bits (406), Expect = 1e-39
 Identities = 82/82 (100%), Positives = 82/82 (100%)
 Frame = +1

Query: 88  MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 267
           MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR
Sbjct: 1   MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 60

Query: 268 TVMRVPLIIVNSIAIVLLLLFG 333
           TVMRVPLIIVNSIAIVLLLLFG
Sbjct: 61  TVMRVPLIIVNSIAIVLLLLFG 82


  Database: RefSeq49_BP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 17,681,374
  Number of sequences in database:  33,088
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 25,858,715
Number of extensions: 544658
Number of successful extensions: 2103
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 2099
Number of HSP's successfully gapped: 3
Length of query: 305
Length of database: 17,681,374
Length adjustment: 102
Effective length of query: 203
Effective length of database: 14,306,398
Effective search space: 2904198794
Effective search space used: 2904198794
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

Search to RefSeqCP_Rel49

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008978
         (917 letters)

Database: RefSeq49_CP.fasta 
           33,336 sequences; 18,874,504 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|XP_855203.1| PREDICTED: similar to immediate early response ...   158   6e-39

>ref|XP_855203.1| PREDICTED: similar to immediate early response 3 interacting
           protein 1 [Canis familiaris].
          Length = 82

 Score =  158 bits (400), Expect = 6e-39
 Identities = 80/82 (97%), Positives = 81/82 (98%)
 Frame = +1

Query: 88  MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 267
           MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWG DQGIGGFGEEPGIKSQLMNLIRSVR
Sbjct: 1   MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGADQGIGGFGEEPGIKSQLMNLIRSVR 60

Query: 268 TVMRVPLIIVNSIAIVLLLLFG 333
           TVMRVPLII+NSIAIVLLLLFG
Sbjct: 61  TVMRVPLIIINSIAIVLLLLFG 82


  Database: RefSeq49_CP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 18,874,504
  Number of sequences in database:  33,336
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 27,036,206
Number of extensions: 563524
Number of successful extensions: 1945
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1943
Number of HSP's successfully gapped: 1
Length of query: 305
Length of database: 18,874,504
Length adjustment: 102
Effective length of query: 203
Effective length of database: 15,474,232
Effective search space: 3141269096
Effective search space used: 3141269096
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

Search to RefSeqSP_Rel49

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008978
         (917 letters)

Database: RefSeq49_SP.fasta 
           24,897 sequences; 11,343,932 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|XP_003353762.1| PREDICTED: immediate early response 3-intera...   160   7e-40
Alignment   gi|XP_003361502.1| PREDICTED: hypothetical protein LOC100627305...   110   1e-24

>ref|XP_003353762.1| PREDICTED: immediate early response 3-interacting protein 1-like
           [Sus scrofa].
          Length = 82

 Score =  160 bits (406), Expect = 7e-40
 Identities = 82/82 (100%), Positives = 82/82 (100%)
 Frame = +1

Query: 88  MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 267
           MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR
Sbjct: 1   MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 60

Query: 268 TVMRVPLIIVNSIAIVLLLLFG 333
           TVMRVPLIIVNSIAIVLLLLFG
Sbjct: 61  TVMRVPLIIVNSIAIVLLLLFG 82


>ref|XP_003361502.1| PREDICTED: hypothetical protein LOC100627305, partial [Sus scrofa].
          Length = 52

 Score =  110 bits (274), Expect = 1e-24
 Identities = 52/52 (100%), Positives = 52/52 (100%)
 Frame = +3

Query: 267 NRDESAIDNSKLNCNCITFTIWVKIHRGNGDSSEEELPAEVTTSVFVGIYIS 422
           NRDESAIDNSKLNCNCITFTIWVKIHRGNGDSSEEELPAEVTTSVFVGIYIS
Sbjct: 1   NRDESAIDNSKLNCNCITFTIWVKIHRGNGDSSEEELPAEVTTSVFVGIYIS 52


  Database: RefSeq49_SP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 11,343,932
  Number of sequences in database:  24,897
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 16,556,056
Number of extensions: 348284
Number of successful extensions: 1375
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1369
Number of HSP's successfully gapped: 2
Length of query: 305
Length of database: 11,343,932
Length adjustment: 99
Effective length of query: 206
Effective length of database: 8,879,129
Effective search space: 1829100574
Effective search space used: 1829100574
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 33 (17.3 bits)

Search to RefSeqMP_Rel49

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008978
         (917 letters)

Database: RefSeq49_MP.fasta 
           30,036 sequences; 15,617,559 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|NP_079685.1| immediate early response 3-interacting protein ...   158   5e-39

>ref|NP_079685.1| immediate early response 3-interacting protein 1 [Mus musculus].
          Length = 82

 Score =  158 bits (400), Expect = 5e-39
 Identities = 80/82 (97%), Positives = 81/82 (98%)
 Frame = +1

Query: 88  MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 267
           MAFTLYSL+QAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR
Sbjct: 1   MAFTLYSLMQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 60

Query: 268 TVMRVPLIIVNSIAIVLLLLFG 333
           TVMRVPLIIVNSI IVLLLLFG
Sbjct: 61  TVMRVPLIIVNSITIVLLLLFG 82


  Database: RefSeq49_MP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 15,617,559
  Number of sequences in database:  30,036
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 22,896,978
Number of extensions: 483031
Number of successful extensions: 1946
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1928
Number of HSP's successfully gapped: 1
Length of query: 305
Length of database: 15,617,559
Length adjustment: 101
Effective length of query: 204
Effective length of database: 12,583,923
Effective search space: 2567120292
Effective search space used: 2567120292
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

Search to RefSeqHP_Rel49

BLASTX 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008978
         (917 letters)

Database: RefSeq49_HP.fasta 
           32,964 sequences; 18,297,164 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Alignment   gi|NP_057181.1| immediate early response 3-interacting protein ...   160   1e-39

>ref|NP_057181.1| immediate early response 3-interacting protein 1 [Homo sapiens].
          Length = 82

 Score =  160 bits (406), Expect = 1e-39
 Identities = 82/82 (100%), Positives = 82/82 (100%)
 Frame = +1

Query: 88  MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 267
           MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR
Sbjct: 1   MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVR 60

Query: 268 TVMRVPLIIVNSIAIVLLLLFG 333
           TVMRVPLIIVNSIAIVLLLLFG
Sbjct: 61  TVMRVPLIIVNSIAIVLLLLFG 82


  Database: RefSeq49_HP.fasta
    Posted date:  Oct 17, 2011  1:42 PM
  Number of letters in database: 18,297,164
  Number of sequences in database:  32,964
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 26,640,836
Number of extensions: 560765
Number of successful extensions: 2252
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 2249
Number of HSP's successfully gapped: 1
Length of query: 305
Length of database: 18,297,164
Length adjustment: 102
Effective length of query: 203
Effective length of database: 14,934,836
Effective search space: 3031771708
Effective search space used: 3031771708
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)

Search to Sscrofa10_2

BLASTN 2.2.24 [Aug-08-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 20110601C-008978
         (917 letters)

Database: Sscrofa_10.2.fasta 
           4582 sequences; 2,808,509,378 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Sscrofa_Chr01                                                        1166   0.0  
Sscrofa_Chr15                                                          66   4e-08

>Sscrofa_Chr01 
||          Length = 315321322

 Score = 1166 bits (588), Expect = 0.0
 Identities = 622/639 (97%)
 Strand = Plus / Plus

                                                                             
Query: 279       agtgccattgataatagtaaactcaattgcaattgtattacttttactatttgggtgaag 338
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309590964 agtgccattgataatagtaaactcaattgcaattgtattacttttactatttgggtgaag 309591023

                                                                             
Query: 339       atccacagaggaaatggagactcatcagaggaagagctgccagcagaagttactacttca 398
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591024 atccacagaggaaatggagactcatcagaggaagagctgccagcagaagttactacttca 309591083

                                                                             
Query: 399       gtctttgttggaatatacatatcttagctggctctccttggacttgacaaagtgtaaacc 458
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591084 gtctttgttggaatatacatatcttagctggctctccttggacttgacaaagtgtaaacc 309591143

                                                                             
Query: 459       tgagagtaaaaccagagtccctatttatctgattttttaaaatattgcacttatggtttc 518
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591144 tgagagtaaaaccagagtccctatttatctgattttttaaaatattgcacttatggtttc 309591203

                                                                             
Query: 519       attacaaaaaggtcagatcatcagaggcagtaactatgcaggattggaatacatttgatt 578
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591204 attacaaaaaggtcagatcatcagaggcagtaactatgcaggattggaatacatttgatt 309591263

                                                                             
Query: 579       gctaacttttaataaaagttcacgctatggtaaagattgtttaatgggtacaggactgtc 638
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591264 gctaacttttaataaaagttcacgctatggtaaagattgtttaatgggtacaggactgtc 309591323

                                                                             
Query: 639       acttatgtgttattggagatttttctctttcacttctcagggatgaannnnnnnnnncaa 698
                 |||||||||||||||||||||||||||||||||||||||||||||||          |||
Sbjct: 309591324 acttatgtgttattggagatttttctctttcacttctcagggatgaattttttttttcaa 309591383

                                                                             
Query: 699       agttttgaagttccttgtgacttagttatgatgtgagaatgattatccttctagacaaaa 758
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591384 agttttgaagttccttgtgacttagttatgatgtgagaatgattatccttctagacaaaa 309591443

                                                                             
Query: 759       tttaaacannnnnnnaaaaagttattactacatataaagtgattttattttgagctcttt 818
                 ||||||||       |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591444 tttaaacatttttttaaaaagttattactacatataaagtgattttattttgagctcttt 309591503

                                                                             
Query: 819       ggttaaatattttgtctgtatactgtctcatatgaaaaatcaaatttttgctaactattg 878
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309591504 ggttaaatattttgtctgtatactgtctcatatgaaaaatcaaatttttgctaactattg 309591563

                                                        
Query: 879       aggagtaatactctctgatttcagggagaattctaattt 917
                 |||||||||||||||||||||||||||||||||||||||
Sbjct: 309591564 aggagtaatactctctgatttcagggagaattctaattt 309591602



 Score =  353 bits (178), Expect = 1e-94
 Identities = 178/178 (100%)
 Strand = Plus / Plus

                                                                             
Query: 1         ggctgccgtaggaagtgccggcggtggtggcgccacgtcccctgccgcggcgggagaggg 60
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309569725 ggctgccgtaggaagtgccggcggtggtggcgccacgtcccctgccgcggcgggagaggg 309569784

                                                                             
Query: 61        atcggctgggccttggggcggttggctatggcctttacgctgtactcgctgctgcaggca 120
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309569785 atcggctgggccttggggcggttggctatggcctttacgctgtactcgctgctgcaggca 309569844

                                                                           
Query: 121       gccctgctctgcgtcaatgccatcgccgtgctgcacgaggagcgcttcctcaagaaca 178
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309569845 gccctgctctgcgtcaatgccatcgccgtgctgcacgaggagcgcttcctcaagaaca 309569902



 Score =  202 bits (102), Expect = 2e-49
 Identities = 102/102 (100%)
 Strand = Plus / Plus

                                                                             
Query: 179       ttggctggggaacagaccagggaattggtggattcggagaagagccaggaattaaatctc 238
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309589291 ttggctggggaacagaccagggaattggtggattcggagaagagccaggaattaaatctc 309589350

                                                           
Query: 239       aactaatgaatctgattcgatctgtaagaaccgtgatgagag 280
                 ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 309589351 aactaatgaatctgattcgatctgtaagaaccgtgatgagag 309589392


>Sscrofa_Chr15 
||          Length = 157681621

 Score = 65.9 bits (33), Expect = 4e-08
 Identities = 48/53 (90%)
 Strand = Plus / Minus

                                                                      
Query: 289       ataatagtaaactcaattgcaattgtattacttttactatttgggtgaagatc 341
                 |||| |||||||||||||| |||||| |||||||||  |||||||||||||||
Sbjct: 123378490 ataacagtaaactcaattgaaattgtgttacttttatgatttgggtgaagatc 123378438


  Database: Sscrofa_10.2.fasta
    Posted date:  Nov 16, 2011 10:34 AM
  Number of letters in database: 2,808,509,378
  Number of sequences in database:  4582
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 31,850,715
Number of extensions: 410
Number of successful extensions: 410
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 409
Number of HSP's successfully gapped: 5
Length of query: 917
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 896
Effective length of database: 2,808,413,156
Effective search space: 2516338187776
Effective search space used: 2516338187776
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 29 (58.0 bits)