BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-013356
(1215 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001030467.1| NADPH--cytochrome P450 reductase [Bos taurus]. 700 0.0
Alignment gi|NP_001039623.2| NADPH-dependent diflavin oxidoreductase 1 [B... 99 8e-21
Alignment gi|NP_001179682.1| tRNA wybutosine-synthesizing protein 1 homol... 93 4e-19
Alignment gi|XP_002698155.1| PREDICTED: tRNA-yW synthesizing protein 1 ho... 93 4e-19
Alignment gi|XP_002694631.1| PREDICTED: nitric oxide synthase 1 (neuronal... 90 5e-18
Alignment gi|XP_872723.2| PREDICTED: nitric oxide synthase 1, neuronal is... 83 4e-16
Alignment gi|NP_001025470.1| methionine synthase reductase [Bos taurus]. 80 4e-15
Alignment gi|NP_851380.2| nitric oxide synthase, endothelial [Bos taurus]. 79 8e-15
Alignment gi|NP_001070267.1| nitric oxide synthase, inducible [Bos taurus]. 74 3e-13
>ref|NP_001030467.1| NADPH--cytochrome P450 reductase [Bos taurus].
Length = 680
Score = 700 bits (1806), Expect = 0.0
Identities = 341/385 (88%), Positives = 358/385 (92%)
Frame = +1
Query: 61 ISMGDSNVDAGTTTSEMVAEEVSLFSATDMVLFSLIVGLLTYWFIFRKKKDEVPEFTKIE 240
++M DSN+DAGTTTSEMVAEEVSLFS TD++LFSLIVG++TYWF+FRKKK+EVPEFTKI+
Sbjct: 1 MNMADSNMDAGTTTSEMVAEEVSLFSTTDVILFSLIVGVMTYWFLFRKKKEEVPEFTKIQ 60
Query: 241 TTTSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYD 420
TTTSSVKD SFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYD
Sbjct: 61 TTTSSVKDRSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYD 120
Query: 421 LSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTY 600
L+DLSSLPEIE ALA+FCMATYGEGDPTDNAQDFYDWLQE DVDLSGVKYAVF LGNKTY
Sbjct: 121 LADLSSLPEIEKALAIFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKYAVFALGNKTY 180
Query: 601 EHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGE 780
EHFNAMGKYVDKRLEQLGAQRIF FITWREQFWPAVCEHFGVEATGE
Sbjct: 181 EHFNAMGKYVDKRLEQLGAQRIFDLGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATGE 240
Query: 781 ESSIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTER 960
ESSIRQYEL+VHTDMD A VYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTER
Sbjct: 241 ESSIRQYELMVHTDMDMAKVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTER 300
Query: 961 HLMHLELGISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQR 1140
HLMHLEL ISDSKIRYESGDHVAVYPANDSALVNQLGEILG DLDI+MSLNNLDEES +
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-013356
(1215 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001171276.1| NADPH--cytochrome P450 reductase [Canis lupu... 682 0.0
Alignment gi|XP_548355.2| PREDICTED: similar to NADPH dependent diflavin ... 100 4e-21
Alignment gi|XP_536836.2| PREDICTED: similar to radical S-adenosyl methio... 94 3e-19
Alignment gi|NP_001182074.1| nitric oxide synthase, brain [Canis lupus fa... 80 4e-15
Alignment gi|XP_535797.2| PREDICTED: similar to methionine synthase reduc... 74 4e-13
Alignment gi|NP_001003186.1| nitric oxide synthase, inducible [Canis lupu... 73 6e-13
>ref|NP_001171276.1| NADPH--cytochrome P450 reductase [Canis lupus familiaris].
Length = 678
Score = 682 bits (1759), Expect = 0.0
Identities = 333/383 (86%), Positives = 347/383 (90%)
Frame = +1
Query: 67 MGDSNVDAGTTTSEMVAEEVSLFSATDMVLFSLIVGLLTYWFIFRKKKDEVPEFTKIETT 246
M DS++DA T SE V EEVSLFS TDM+LFSLIVGLLTYWF+FRKKKDE+PEFT I+
Sbjct: 1 MEDSSMDASATVSETVVEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKDEIPEFTTIQPM 60
Query: 247 TSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLS 426
TSS KDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDL+
Sbjct: 61 TSSAKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLA 120
Query: 427 DLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEH 606
DL SLPEIEN+LAVFCMATYGEGDPTDNAQDFYDWLQE DVDLSGVKYAVFGLGNKTYEH
Sbjct: 121 DLGSLPEIENSLAVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKYAVFGLGNKTYEH 180
Query: 607 FNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEES 786
FNAMGKYVDKRLEQLGAQRIF FITWREQFWPAVCEHFGVEATGEES
Sbjct: 181 FNAMGKYVDKRLEQLGAQRIFELGMGDDDGNLEEDFITWREQFWPAVCEHFGVEATGEES 240
Query: 787 SIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHL 966
SIRQYELVVHTD+D A VY GEMGRLKSYENQKPPFDAKNPFLA VTTNRKLNQGTERHL
Sbjct: 241 SIRQYELVVHTDIDMAKVYVGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHL 300
Query: 967 MHLELGISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHP 1146
MHLEL ISDSK+RYESGDHVAVYPANDSALVNQLGEILG DLD+VMSLNNLDEES ++HP
Sbjct: 301 MHLELDISDSKLRYESGDHVAVYPANDSALVNQLGEILGADLDVVMSLNNLDEESNKKHP 360
Query: 1147 FPCPTTYRTALTYYLDIHQPPRT 1215
FPCPT+YRTALTYYLDI PPRT
Sbjct: 361 FPCPTSYRTALTYYLDITNPPRT 383
>ref|XP_548355.2| PREDICTED: similar to NADPH dependent diflavin oxidoreductase 1
[Canis familiaris].
Length = 644
Score = 100 bits (248), Expect = 4e-21
Identities = 84/315 (26%), Positives = 141/315 (44%), Gaps = 12/315 (3%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMAT 483
++V +GSQTGTA++ A RL +DA R + + +
Sbjct: 301 HLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGEILGADLDIIMSLNNLDEESNKK 360
Query: 1141 HPFPCPTTYRTALTYYLDIHQPPRT 1215
HPFPCPT+YRTALTYYLDI PPRT
Sbjct: 361 HPFPCPTSYRTALTYYLDITNPPRT 385
>ref|NP_001039623.2| NADPH-dependent diflavin oxidoreductase 1 [Bos taurus].
Length = 597
Score = 99.0 bits (245), Expect = 8e-21
Identities = 84/312 (26%), Positives = 144/312 (46%), Gaps = 9/312 (2%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMAT 483
++V +GSQTGTA++ + RL ++A R R ++ EE D + +L I L +F AT
Sbjct: 6 LLVLFGSQTGTAQDVSERLGREARR---RQLSCRVEELDSYPVVNL--INEPLVIFVCAT 60
Query: 484 YGEGDPTDNAQDFYDWLQEADVDLSGVK---YAVFGLGNKTYEHFNAMGKYVDKRLEQLG 654
G+GDP DN + F+ ++ + + ++ +AV GLG+ +Y FN + K + +RL QLG
Sbjct: 61 TGQGDPPDNMKSFWRFIFRRSLPSTALRQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLG 120
Query: 655 AQRIF--XXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEESS---IRQYELVVHT 819
+ W + W V V + S + + +
Sbjct: 121 GSALLPVCLGDDQHELGPDAAIDPWLQDLWEKVLGPHPVPLNLDLSPPGVLWPSKFTLQF 180
Query: 820 DMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLELGISDS 996
DT E+ + + Q PP + + PFLA + +N+++ + + + +E IS S
Sbjct: 181 LKDTPSSGPEELCAAGT-DPQGPPSELQ-PFLAPMVSNQRVTGPSHFQDVRLIEFDISGS 238
Query: 997 KIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPTTYRTA 1176
I + +GD V + P N ++ V Q + LG D + +L + P P + R
Sbjct: 239 GISFAAGDLVLIQPENTASHVQQFCQALGLDPEQHFTLQPREPGVTCPTRLPQPCSVRRL 298
Query: 1177 LTYYLDIHQPPR 1212
++ YLDI PR
Sbjct: 299 VSQYLDIASVPR 310
>ref|NP_001179682.1| tRNA wybutosine-synthesizing protein 1 homolog [Bos taurus].
Length = 731
Score = 93.2 bits (230), Expect = 4e-19
Identities = 59/173 (34%), Positives = 90/173 (52%), Gaps = 10/173 (5%)
Frame = +1
Query: 178 LTYWFIFRKKKDEVPEFTKIETTTSSVKDSSFVEKMKKTG--RNIIVFYGSQTGTAEEFA 351
+ + + RK+ PE T + + ++ + ++ + KK + +FYGSQTGTA+ FA
Sbjct: 36 ICFQIVIRKQNKNSPEKTTPKAS-QNLMTNGYISRPKKEVFVSGVKIFYGSQTGTAKGFA 94
Query: 352 NRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDW 531
L++ + + ++YD D + F +ATY +G P ++A F W
Sbjct: 95 TILAEALSSLNLPVAMINLKDYDPDDRLIEEVTSKNVCAFLVATYTDGQPPESAAWFCKW 154
Query: 532 LQEADVD-------LSGVKYAVFGLGNKTY-EHFNAMGKYVDKRLEQLGAQRI 666
L+EA D L G++YAVFGLGN Y HFN +GK VDK L LGA R+
Sbjct: 155 LEEAANDFRFGKTYLKGMRYAVFGLGNSVYLSHFNTVGKNVDKWLWMLGAHRV 207
>ref|XP_002698155.1| PREDICTED: tRNA-yW synthesizing protein 1 homolog [Bos taurus].
Length = 675
Score = 93.2 bits (230), Expect = 4e-19
Identities = 59/173 (34%), Positives = 90/173 (52%), Gaps = 10/173 (5%)
Frame = +1
Query: 178 LTYWFIFRKKKDEVPEFTKIETTTSSVKDSSFVEKMKKTG--RNIIVFYGSQTGTAEEFA 351
+ + + RK+ PE T + + ++ + ++ + KK + +FYGSQTGTA+ FA
Sbjct: 36 ICFQIVIRKQNKNSPEKTTPKAS-QNLMTNGYISRPKKEVFVSGVKIFYGSQTGTAKGFA 94
Query: 352 NRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDW 531
L++ + + ++YD D + F +ATY +G P ++A F W
Sbjct: 95 TILAEALSSLNLPVAMINLKDYDPDDRLIEEVTSKNVCAFLVATYTDGQPPESAAWFCKW 154
Query: 532 LQEADVD-------LSGVKYAVFGLGNKTY-EHFNAMGKYVDKRLEQLGAQRI 666
L+EA D L G++YAVFGLGN Y HFN +GK VDK L LGA R+
Sbjct: 155 LEEAANDFRFGKTYLKGMRYAVFGLGNSVYLSHFNTVGKNVDKWLWMLGAHRV 207
>ref|XP_002694631.1| PREDICTED: nitric oxide synthase 1 (neuronal) [Bos taurus].
Length = 1232
Score = 89.7 bits (221), Expect = 5e-18
Identities = 72/269 (26%), Positives = 122/269 (45%), Gaps = 12/269 (4%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENAY + +L I L VF AT
Sbjct: 53 LLVLFGSQTGTAQDVAERLGRDARRRRLDCRVQALDSYSVVNL-----INEPLVVFVCAT 107
Query: 484 YGEGDPTDNAQDFYDWLQEADV---DLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLG 654
G+G+P DN ++F+ ++ ++ L + +AV GLG+ +Y FN + K + +RL QLG
Sbjct: 108 AGQGEPPDNMKNFWRFIFRKNLPSTSLCQMDFAVLGLGDSSYAKFNFVAKKLYRRLLQLG 167
Query: 655 AQRIFXXXXXXXXXXXXXXFIT--WREQFWPAVC------EHFGVEATGEESSIRQYELV 810
+ W W V GV TG
Sbjct: 168 GSALLPLCLGDDQHELGPDAAVDPWLHDLWEKVLWLYPVPPDLGVIPTGVP---LPSTFT 224
Query: 811 VHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLELGI 987
+H ++ + E S + Q PP + + PFLA + TN+++ + + + +E I
Sbjct: 225 LHFLLEAPRTCSEEQ-HGASTDPQGPPSE-RQPFLAPMVTNQRVTGPSHFQDVRLIEFDI 282
Query: 988 SDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPTTY 1167
+ S + + +GD V + P N ++ + + + LG D D +L + P P +
Sbjct: 283 TGSGLSFAAGDVVLIQPENRASDLGRFCQALGLDPDQYFTLQPREPGVPCPAQLPQPCSV 342
Query: 1168 RTALTYYLDIHQPPR 1212
R + +YLDI PR
Sbjct: 343 RHLVAHYLDIASVPR 357
>ref|XP_536836.2| PREDICTED: similar to radical S-adenosyl methionine and flavodoxin
domains 1 [Canis familiaris].
Length = 714
Score = 93.6 bits (231), Expect = 3e-19
Identities = 62/170 (36%), Positives = 87/170 (51%), Gaps = 11/170 (6%)
Frame = +1
Query: 190 FIFRKKKDE---VPEFTKIETTTSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRL 360
FI + KK + VP+ T T + ++ + + +FYGSQTGTA+ FA L
Sbjct: 47 FILKGKKSQEKSVPKATGDLMTNGCIS----LQNKEVFVSGVKIFYGSQTGTAKGFATSL 102
Query: 361 SKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQE 540
++ + + +EYD D + F +ATY +G PT++A F WL+E
Sbjct: 103 AEAVTSLDLPVAVINLKEYDPDDQLVEEVTSKNVCAFVVATYTDGQPTESAVWFCKWLEE 162
Query: 541 ADVD-------LSGVKYAVFGLGNKTY-EHFNAMGKYVDKRLEQLGAQRI 666
A D L G++YAVFGLGN Y HFN +GK VDK L LGA R+
Sbjct: 163 ASSDFRFGKTYLKGMRYAVFGLGNSVYASHFNKVGKNVDKWLWMLGAHRV 212
>ref|NP_001182074.1| nitric oxide synthase, brain [Canis lupus familiaris].
Length = 1431
Score = 80.1 bits (196), Expect = 4e-15
Identities = 87/378 (23%), Positives = 142/378 (37%), Gaps = 78/378 (20%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYG 489
+ Y ++TG ++ +A L + ++ EEYD+ L L + +T+G
Sbjct: 759 ILYATETGKSQAYAKTLC-EIFKHAFDAKVMSMEEYDIVHLE-----HETLVLVVTSTFG 812
Query: 490 EGDPTDNAQDFYDWLQE------------------------------------------A 543
GDP +N + F L E +
Sbjct: 813 NGDPPENGEKFGCALMEMRHPNSVHEERKSYKVRFNSVSSYSDSHKSSGDGPDLRDNFES 872
Query: 544 DVDLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITW 723
L+ V+++VFGLG++ Y HF A G VD LE+LG +RI F TW
Sbjct: 873 AGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTW 932
Query: 724 REQFWPAVCEHFGVEATGEESSI-RQYELVVHTDMD--------TAVVYTGEM------- 855
++ + A C+ F V G++ +I + ++ D T V E+
Sbjct: 933 AKKVFKAACDVFCV---GDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNV 989
Query: 856 -------GRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHLMHLELGISDSKIRYES 1014
RL S +N + P +++ + TN + +++Y+
Sbjct: 990 HKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTN------------------GNQELQYQP 1031
Query: 1015 GDHVAVYPANDSALVNQLGEIL--GTDLDIVMSLNNLDEESXQ-----------RHPFPC 1155
GDH+ V+P N LVN L E L ++ ++ + L+E + R P
Sbjct: 1032 GDHLGVFPGNREDLVNALMERLEDAPPVNQLVKVELLEERNTALGVISNWTDELRLP--- 1088
Query: 1156 PTTYRTALTYYLDIHQPP 1209
P T A YYLDI PP
Sbjct: 1089 PCTIFQAFKYYLDITTPP 1106
>ref|XP_535797.2| PREDICTED: similar to methionine synthase reductase isoform 2
LAVFCMATYG 489
+ Y ++TG ++ +A L + ++ EEYD+ L L + +T+G
Sbjct: 621 ILYATETGKSQAYAKTLC-EIFKHAFDAKVMSMEEYDIVHLE-----HETLVLVVTSTFG 674
Query: 490 EGDPTDNAQDFYDWLQEA--DVDLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQR 663
GDP +N ++ L + + + G K++VFGLG++ Y HF A G VD LE+LG +R
Sbjct: 675 NGDPPENGEELKSLLMKVKEESEKVGFKFSVFGLGSRAYPHFCAFGHAVDTLLEELGGER 734
Query: 664 IFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEESSI-RQYELVVHTDMD---- 828
I F TW ++ + A C+ F V G++ +I + ++ D
Sbjct: 735 ILKMREGDELCGQEEAFRTWAKKVFKAACDVFCV---GDDVNIEKANNSLISNDRSWKRN 791
Query: 829 ----TAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHLMHLELGISDS 996
T V E+ + S ++K A+ L + T R + + L + S
Sbjct: 792 KFRLTYVAEAPELTQGLSSVHKKRVSAAR---LLSRQNLQSPKSRTSRSTIFVRLHTNGS 848
Query: 997 -KIRYESGDHVAVYPANDSALVNQLGEIL 1080
+++Y+ GDH+ V+P N LVN L E L
Sbjct: 849 QELQYQPGDHLGVFPGNHEDLVNALIERL 877
>ref|XP_872723.2| PREDICTED: nitric oxide synthase 1, neuronal isoform 2 [Bos taurus].
Length = 1430
Score = 83.2 bits (204), Expect = 4e-16
Identities = 86/363 (23%), Positives = 145/363 (39%), Gaps = 63/363 (17%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYG 489
+ Y ++TG ++ +A L + ++ EEYD+ L L + +T+G
Sbjct: 762 ILYATETGKSQAYAKTLC-EIFKHAFDAKVMSMEEYDIVHLE-----HETLVLVVTSTFG 815
Query: 490 EGDPTDNAQDFYDWLQE------------------------------------------A 543
GDP +N + F L E +
Sbjct: 816 NGDPPENGEKFGCALMEMRNPNSVHEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFES 875
Query: 544 DVDLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITW 723
L+ V+++VFGLG++ Y HF A G VD LE+LG +RI F TW
Sbjct: 876 TGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTW 935
Query: 724 REQFWPAVCEHFGVEATGEESSI-RQYELVVHTDMD--------TAVVYTGEMGRLKSYE 876
++ + A C+ F V G++ +I + ++ D T V E+ + S
Sbjct: 936 AKKVFKAACDVFCV---GDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSSV 992
Query: 877 NQKPPFDAKNPFLAVVTTNRKLNQGTERHLMHLELGISDS-KIRYESGDHVAVYPANDSA 1053
++K A+ +++ + + R + + L + S +++Y+ GDH+ V+P N
Sbjct: 993 HKKRVSAAR-----LLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHED 1047
Query: 1054 LVNQLGEIL--GTDLDIVMSLNNLDEES---------XQRHPFPCPTTYRTALTYYLDIH 1200
LVN L E L + ++ + L+E + H P P T A YYLDI
Sbjct: 1048 LVNALIERLEDAPPANQLVKVELLEERNTALGVISNWTDEHRLP-PCTIFQAFKYYLDIT 1106
Query: 1201 QPP 1209
PP
Sbjct: 1107 TPP 1109
>ref|NP_001025470.1| methionine synthase reductase [Bos taurus].
Length = 695
Score = 80.1 bits (196), Expect = 4e-15
Identities = 52/180 (28%), Positives = 81/180 (45%), Gaps = 3/180 (1%)
Frame = +1
Query: 298 RNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
R ++ Y +Q G A+ A +S+ A YG E D DL + E A V +
Sbjct: 2 RRFLLLYATQRGQAKAIAEEISEKAVTYGFSADLHCISESDKYDLKT----ETAPLVMVV 57
Query: 478 ATYGEGDPTDNAQDFYDWLQEADVD---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
+T G GDP D A+ F +++ + L+ ++Y + GLG+ Y +F GK +DKRL++
Sbjct: 58 STTGNGDPPDTARKFVKAIKDKTLPPDFLAHLRYGLLGLGDSEYTYFCNGGKVIDKRLQE 117
Query: 649 LGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTDMD 828
LGAQR + W W A+ +HF + G E + + H D
Sbjct: 118 LGAQRFYDTGHADDCVGLELVVEPWINGLWAALEKHF-LSNRGREDTSETLTMASHASRD 176
>ref|NP_851380.2| nitric oxide synthase, endothelial [Bos taurus].
Length = 1205
Score = 79.0 bits (193), Expect = 8e-15
Identities = 100/417 (23%), Positives = 158/417 (37%), Gaps = 78/417 (18%)
Frame = +1
Query: 193 IFRKKK-DEVPEFTKIETTTSSVKDSSFVEKMKKTGRNIIVFYGS [Canis familiaris].
Length = 697
Score = 73.6 bits (179), Expect = 4e-13
Identities = 45/157 (28%), Positives = 72/157 (45%), Gaps = 3/157 (1%)
Frame = +1
Query: 298 RNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
R ++ Y +Q G A+ A + + A +G E D DL + E A V +
Sbjct: 2 RRFLLLYATQKGQAKAIAEEICEKAFAHGFSADLHCISESDKYDLKT----ETAPLVVVV 57
Query: 478 ATYGEGDPTDNAQDFYDWLQEADVDL---SGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
+T G GDP D A+ F +Q+ + + + ++Y + GLG+ Y +F GK +DKRL++
Sbjct: 58 STTGTGDPPDTARKFVKEIQDKALPIDFFAHLRYGLLGLGDSEYTYFCNGGKIIDKRLQE 117
Query: 649 LGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHF 759
LGAQ + W W A+ +HF
Sbjct: 118 LGAQHFYDTGHADDCVGLELVVEPWINGLWAALTKHF 154
>ref|NP_001003186.1| nitric oxide synthase, inducible [Canis lupus familiaris].
Length = 1154
Score = 72.8 bits (177), Expect = 6e-13
Identities = 80/329 (24%), Positives = 130/329 (39%), Gaps = 14/329 (4%)
Frame = +1
Query: 265 SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLP 444
S + K + + + + ++TG +E A L +EY LS L
Sbjct: 523 SMLMRKTMASRVRVTILFATETGKSETLARDLGA-LFSCAFHPKVLCMDEYKLSHLE--- 578
Query: 445 EIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEHFNAMGK 624
E L + +T+G GD N + L + +YAVFGL + Y F A
Sbjct: 579 --EEQLLLVVTSTFGNGDSPGNGEKLKKSLFMLKELTNNFRYAVFGLRSNMYPQFCAFAH 636
Query: 625 YVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEESSIRQYE 804
+D +L LGA ++ F W Q + A C + S +R
Sbjct: 637 DIDHKLSHLGASQLTPGGEGDELNGKEEAFRCWAVQTFKAAC---------DTSDVRGKH 687
Query: 805 LVVHTDMDTAVVYTGEMGRLKSYENQKP--------PFDAKNPFLAVVTTNRKLNQG-TE 957
+ + T+ V T + + ++ +P AKN F + + R L +
Sbjct: 688 CIQIPRLYTSNV-TWDPHHYRLLQDSQPLDLNKALSKMHAKNVFTLRLKSQRNLQSPISN 746
Query: 958 RHLMHLELGISDS-KIRYESGDHVAVYPANDSALVNQLGE-ILGTDLDI-VMSLNNLDEE 1128
R + +EL DS ++ Y G+H+ V+P N ALV + E ++ + + + L L E
Sbjct: 747 RTTLQVELSCEDSQELSYLPGEHLGVFPGNQLALVQGILERVVYSPAPLQPVHLETLSER 806
Query: 1129 SXQ--RHPFPCPTTYRTALTYYLDIHQPP 1209
R+ P + ALTY+LDI PP
Sbjct: 807 GSYWVRNNRLPPCSLSQALTYFLDITTPP 835
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.317 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 46,661,661
Number of extensions: 1270331
Number of successful extensions: 5173
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 5157
Number of HSP's successfully gapped: 6
Length of query: 405
Length of database: 18,874,504
Length adjustment: 105
Effective length of query: 300
Effective length of database: 15,374,224
Effective search space: 4612267200
Effective search space used: 4612267200
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
QTGTAEEFANRLSKD 369
I RKK EV KI ++S+ + +++K T + Y S+TG A+ +A +L +
Sbjct: 492 ITRKKTFKEVANAVKI---SASLMGTLMAKRVKAT-----ILYASETGRAQSYAQQLGRL 543
Query: 370 AHRYGMRGMAADP-----EEYDLSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWL 534
+ A DP +EYD+ L AL + +T+G GDP +N + F L
Sbjct: 544 FRK------AFDPRVLCMDEYDVVSLE-----HEALVLVVTSTFGNGDPPENGESFAAAL 592
Query: 535 QE-----------------------------------------ADVDLSG----VKYAVF 579
E ++ D +G +++ VF
Sbjct: 593 MEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVF 652
Query: 580 GLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHF 759
GLG++ Y HF A + VD RLE+LG +R+ F W + + A CE F
Sbjct: 653 GLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAKAAFQASCETF 712
Query: 760 GVEATGEESSIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVV----- 924
V GEE+ ++ +S++ Q+ + L ++
Sbjct: 713 CV---GEEAKAAAQDI---------------FSPKRSWKRQRYRLSTQAEGLQLLPGLIH 754
Query: 925 TTNRKLNQGTERHLMHLELGIS-------------DSKIRYESGDHVAVYPANDSALVNQ 1065
RK+ Q T + +L+ S ++Y+ GDH+ + P N LV
Sbjct: 755 VHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPGDHIGICPPNRPGLVEA 814
Query: 1066 LGEIL-----GTDLDIVMSLNNLD----EESXQRHPFPCPTTYRTALTYYLDIHQPP 1209
L + T+ V L S R P P T R ALT++LDI PP
Sbjct: 815 LLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCTLRQALTFFLDITSPP 871
>ref|NP_001070267.1| nitric oxide synthase, inducible [Bos taurus].
Length = 1156
Score = 73.9 bits (180), Expect = 3e-13
Identities = 83/315 (26%), Positives = 127/315 (40%), Gaps = 15/315 (4%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADP-----EEYDLSDLSSLPEIENALAVFC 474
+ + ++TG +E A L + A +P ++Y LS L E L +
Sbjct: 541 ILFATETGRSETLAQDLG------ALFSCAFNPKVLCMDQYQLSHLE-----EEQLLLVV 589
Query: 475 MATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLG 654
+T+G GD N + L + +YAVFGLG+ Y F A +D++L QLG
Sbjct: 590 TSTFGNGDSPGNGEKLKKSLLMLKELTNTFRYAVFGLGSSMYPQFCAFAHDIDQKLSQLG 649
Query: 655 AQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTD-MDT 831
A ++ F +W Q + A CE F V + Y V D
Sbjct: 650 ASQLAPTGEGDELSGQEEAFRSWAVQTFKAACETFDVSGKHHIEIPKLYTSNVTWDPQHY 709
Query: 832 AVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKL-NQGTERHLMHLELGISDSKI-R 1005
+V E L + AK+ F + + + L + + R + +EL S+
Sbjct: 710 RLVQDSEPLDLNKALSS---MHAKHVFTMRLKSQQNLQSPKSSRTTLLVELSCEGSQAPS 766
Query: 1006 YESGDHVAVYPANDSALVNQLGE--ILGTDLDIVMSLNNLDEE-----SXQRHPFPCPTT 1164
Y G+H+ V+P N ALV + E + G + L L E +R P P +
Sbjct: 767 YLPGEHLGVFPCNQPALVQGILERVVDGPAPHQPVRLETLCENGSYWVKDKRLP---PCS 823
Query: 1165 YRTALTYYLDIHQPP 1209
ALTY+LDI PP
Sbjct: 824 LSQALTYFLDITTPP 838
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.317 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 44,569,026
Number of extensions: 1220602
Number of successful extensions: 4949
Number of sequences better than 1.0e-05: 9
Number of HSP's gapped: 4924
Number of HSP's successfully gapped: 9
Length of query: 405
Length of database: 17,681,374
Length adjustment: 105
Effective length of query: 300
Effective length of database: 14,207,134
Effective search space: 4262140200
Effective search space used: 4262140200
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-013356
(1215 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001123431.1| NADPH--cytochrome P450 reductase [Sus scrofa]. 730 0.0
Alignment gi|XP_003122396.1| PREDICTED: LOW QUALITY PROTEIN: NADPH-depend... 104 1e-22
Alignment gi|NP_999460.1| nitric oxide synthase, endothelial [Sus scrofa]. 74 2e-13
Alignment gi|XP_003134196.1| PREDICTED: methionine synthase reductase [Su... 73 3e-13
Alignment gi|NP_001137162.1| nitric oxide synthase, inducible [Sus scrofa]. 65 1e-10
Alignment gi|XP_003358212.1| PREDICTED: nitric oxide synthase, inducible ... 51 1e-06
>ref|NP_001123431.1| NADPH--cytochrome P450 reductase [Sus scrofa].
Length = 678
Score = 730 bits (1885), Expect = 0.0
Identities = 363/383 (94%), Positives = 364/383 (95%)
Frame = +1
Query: 67 MGDSNVDAGTTTSEMVAEEVSLFSATDMVLFSLIVGLLTYWFIFRKKKDEVPEFTKIETT 246
MGDSNVD GTTTSEMVAEEVSLFSATDMVLFSLIVGLLTYWFIFRKKKDEVPEFTKIETT
Sbjct: 1 MGDSNVDTGTTTSEMVAEEVSLFSATDMVLFSLIVGLLTYWFIFRKKKDEVPEFTKIETT 60
Query: 247 TSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLS 426
TSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLS
Sbjct: 61 TSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLS 120
Query: 427 DLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEH 606
DLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEH
Sbjct: 121 DLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEH 180
Query: 607 FNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEES 786
FNAMGKYVDKRLEQLGAQRIF FITWREQFWPAVCEHFGVEATGEES
Sbjct: 181 FNAMGKYVDKRLEQLGAQRIFDLGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATGEES 240
Query: 787 SIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHL 966
SIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHL
Sbjct: 241 SIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHL 300
Query: 967 MHLELGISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHP 1146
MHLEL ISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEES +RHP
Sbjct: 301 MHLELDISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESNKRHP 360
Query: 1147 FPCPTTYRTALTYYLDIHQPPRT 1215
FPCPTTYRTALTYYLDI PPRT
Sbjct: 361 FPCPTTYRTALTYYLDITNPPRT 383
>ref|XP_003122396.1| PREDICTED: LOW QUALITY PROTEIN: NADPH-dependent diflavin
oxidoreductase 1-like [Sus scrofa].
Length = 598
Score = 104 bits (259), Expect = 1e-22
Identities = 86/315 (27%), Positives = 142/315 (45%), Gaps = 12/315 (3%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMAT 483
++V +GSQTGTA++ + RL ++A R + + Y + +L + P L +F AT
Sbjct: 6 LLVLFGSQTGTAQDVSERLGREARRRQLCCRVQALDSYPVVNLINEP-----LVIFVCAT 60
Query: 484 YGEGDPTDNAQDFYDWLQEADV---DLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLG 654
G+GDP DN + F+ ++ ++ L + +AV GLG+ +Y FN + K + +RL QLG
Sbjct: 61 TGQGDPPDNMKGFWKFIFRRNLPSTSLCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLG 120
Query: 655 AQRIF--XXXXXXXXXXXXXXFITWREQFWP------AVCEHFGVEATGEESSIRQYELV 810
+ W W AV GV A ++ L
Sbjct: 121 GSALLPVCLGDDQHELGPDATIDPWLRDLWEKVLGPHAVPLDLGVTAPAVPXLALRFTLQ 180
Query: 811 VHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLELGI 987
+ + A E + + Q PP + + PFLA + TN+++ + + + +E I
Sbjct: 181 L---LQEAPRKCSEELSVAGTDPQGPPSELQ-PFLAPMVTNQRVTGPSHFQDVRLIEFDI 236
Query: 988 SDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPTTY 1167
S S I + +GD V + P N ++ V Q ++LG D D L + P P +
Sbjct: 237 SGSGISFAAGDVVLIQPENAASRVQQFCQLLGLDPDQHFMLQPQEPGVPCPERLPQPCSV 296
Query: 1168 RTALTYYLDIHQPPR 1212
R ++ YLDI PR
Sbjct: 297 RRLVSQYLDISSVPR 311
>ref|NP_999460.1| nitric oxide synthase, endothelial [Sus scrofa].
Length = 1205
Score = 73.6 bits (179), Expect = 2e-13
Identities = 86/378 (22%), Positives = 140/378 (37%), Gaps = 78/378 (20%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADP-----EEYDLSDLSSLPEIENALAVFC 474
+ Y S+T A+ +A +L + + A DP +EYD+ L L +
Sbjct: 524 ILYASETVRAQSYAQQLGRLFRK------AFDPRVLCMDEYDVVSLE-----HETLVLVV 572
Query: 475 MATYGEGDPTDNAQDFYDWLQE-------------------------------------- 540
+T+G GDP +N + F L E
Sbjct: 573 TSTFGNGDPPENGESFAAALMEMSGPYNGSPRPEQHRSYKIRFNSVSCSDPLVSSWRRKR 632
Query: 541 ---ADVDLSG----VKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXX 699
++ D +G +++ VFGLG++ Y HF A + VD RLE+LG +R+
Sbjct: 633 KESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCG 692
Query: 700 XXXXFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTDMDTAVVYTGEMGRLKSYEN 879
F W + + A CE F V GE++ ++ +S++
Sbjct: 693 QEEAFRGWAQAAFQASCETFCV---GEDAKAAARDI---------------FSPKRSWKR 734
Query: 880 QKPPFDAKNPFLAVV-----TTNRKLNQGTERHLMHLELGIS-------------DSKIR 1005
Q+ A+ L ++ RK+ Q T + +L+ S ++
Sbjct: 735 QRYRLSAQVEGLQLLPGLVHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTEGQEGLQ 794
Query: 1006 YESGDHVAVYPANDSALVNQL----------GEILGTDLDIVMSLNNLDEESXQRHPFPC 1155
Y+ GDH+ + P N + LV L E +G + + S R P
Sbjct: 795 YQPGDHIGICPPNRTGLVEALLSRVEDPTPPTESVGVE-QLEKGSPGGPPPSWVRDPRLP 853
Query: 1156 PTTYRTALTYYLDIHQPP 1209
P T R ALT++LDI PP
Sbjct: 854 PYTLRQALTFFLDITSPP 871
>ref|XP_003134196.1| PREDICTED: methionine synthase reductase [Sus scrofa].
Length = 694
Score = 73.2 bits (178), Expect = 3e-13
Identities = 46/157 (29%), Positives = 72/157 (45%), Gaps = 3/157 (1%)
Frame = +1
Query: 298 RNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
R ++ Y +Q G A+ A + + A +G E D DL + E A V +
Sbjct: 2 RRFLLLYATQRGQAKAIAEEICEKADAHGFSADLHCISELDKYDLRT----EAAPLVVVV 57
Query: 478 ATYGEGDPTDNAQDFYDWLQEADVD---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
+T G GDP D A+ F +Q+ + L+ ++Y + GLG+ Y +F GK +DKRL++
Sbjct: 58 STTGNGDPPDTARKFVKAIQDKTLPGDLLAHLRYGLLGLGDSEYTYFCNGGKIIDKRLQE 117
Query: 649 LGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHF 759
LGAQ + W W A+ +HF
Sbjct: 118 LGAQHFYDTGHADDCVGLELVVEPWINGLWAALEKHF 154
>ref|NP_001137162.1| nitric oxide synthase, inducible [Sus scrofa].
Length = 1064
Score = 64.7 bits (156), Expect = 1e-10
Identities = 82/331 (24%), Positives = 123/331 (37%), Gaps = 31/331 (9%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADP-----EEYDLSDLSSLPEIENALAVFC 474
+ + ++TG +E A L + A +P +EY LS L E L +
Sbjct: 448 ILFATETGRSETLARDLG------ALFSCAFNPKVLCMDEYRLSRLE-----EEQLLLVV 496
Query: 475 MATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLG 654
+T+G G N + L + +YAVFGLG+ Y F A +D++L LG
Sbjct: 497 TSTFGNGGSPGNGEKLKKSLFMLKELTNKFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLG 556
Query: 655 AQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGV--------------EATGEESSI 792
A ++ F W Q + CE F V T +
Sbjct: 557 ASQLTPTGEGDELSGQEEAFRGWAVQTFKVACETFDVRGKHHIQIPKLYTSNVTWDPQLY 616
Query: 793 RQYELVVHTDMDTAV-----VYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE 957
R + D++ A+ Y M RLKS +N + P ++ L + +QG
Sbjct: 617 RLVQDSEPLDLNKALSSMHAKYVFSM-RLKSQQNLQSPQSSRTTLLVELCCEG--SQGPS 673
Query: 958 RHLMHLELGISDSKIRYESGDHVAVYPANDSALVNQLGE--ILGTDLDIVMSLNNLDEE- 1128
Y G+H+ V+PAN ALV + E + G + L L E
Sbjct: 674 ----------------YLPGEHLGVFPANQPALVQGILERVVDGPAPHQPVRLETLSENG 717
Query: 1129 ----SXQRHPFPCPTTYRTALTYYLDIHQPP 1209
+R P P + LTY+LDI PP
Sbjct: 718 SYWVKDKRLP---PCSLSQVLTYFLDITTPP 745
>ref|XP_003358212.1| PREDICTED: nitric oxide synthase, inducible [Sus scrofa].
Length = 758
Score = 51.2 bits (121), Expect = 1e-06
Identities = 42/157 (26%), Positives = 62/157 (39%), Gaps = 5/157 (3%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADP-----EEYDLSDLSSLPEIENALAVFC 474
+ + ++TG +E A L + A +P +EY LS L E L +
Sbjct: 541 ILFATETGRSETLARDLG------ALFSCAFNPKVLCMDEYRLSRLE-----EEQLLLVV 589
Query: 475 MATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLG 654
+T+G G N + L + +YAVFGLG+ Y F A VD++L LG
Sbjct: 590 TSTFGNGGSPGNGEKLKKSLFMLKELTNKFRYAVFGLGSSMYPQFCAFAHDVDQKLSHLG 649
Query: 655 AQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGV 765
A ++ F W Q + CE F V
Sbjct: 650 ASQLTPTGEGDELSGQEEAFRGWAVQTFKVACETFDV 686
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.317 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 28,769,614
Number of extensions: 789067
Number of successful extensions: 3099
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 3091
Number of HSP's successfully gapped: 7
Length of query: 405
Length of database: 11,343,932
Length adjustment: 101
Effective length of query: 304
Effective length of database: 8,829,335
Effective search space: 2684117840
Effective search space used: 2684117840
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-013356
(1215 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_032924.1| NADPH--cytochrome P450 reductase [Mus musculus]. 668 0.0
Alignment gi|NP_001075945.1| NADPH-dependent diflavin oxidoreductase 1 [M... 102 5e-22
Alignment gi|NP_001015876.1| tRNA wybutosine-synthesizing protein 1 homol... 90 4e-18
Alignment gi|NP_849228.1| tRNA wybutosine-synthesizing protein 1 homolog ... 90 4e-18
Alignment gi|NP_032738.1| nitric oxide synthase, brain [Mus musculus]. 85 1e-16
Alignment gi|NP_032739.3| nitric oxide synthase, endothelial [Mus musculu... 84 2e-16
Alignment gi|NP_766068.1| methionine synthase reductase [Mus musculus]. 74 2e-13
Alignment gi|NP_035057.1| nitric oxide synthase, inducible [Mus musculus]. 74 3e-13
>ref|NP_032924.1| NADPH--cytochrome P450 reductase [Mus musculus].
Length = 678
Score = 668 bits (1724), Expect = 0.0
Identities = 324/383 (84%), Positives = 345/383 (90%)
Frame = +1
Query: 67 MGDSNVDAGTTTSEMVAEEVSLFSATDMVLFSLIVGLLTYWFIFRKKKDEVPEFTKIETT 246
MGDS+ D T E VAEEVSLFS TD+VLFSLIVG+LTYWFIF+KKK+E+PEF+KI+TT
Sbjct: 1 MGDSHEDTSATVPEAVAEEVSLFSTTDIVLFSLIVGVLTYWFIFKKKKEEIPEFSKIQTT 60
Query: 247 TSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLS 426
VK+SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGM+ADPEEYDL+
Sbjct: 61 APPVKESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLA 120
Query: 427 DLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEH 606
DLSSLPEI+ +L VFCMATYGEGDPTDNAQDFYDWLQE DVDL+GVK+AVFGLGNKTYEH
Sbjct: 121 DLSSLPEIDKSLVVFCMATYGEGDPTDNAQDFYDWLQETDVDLTGVKFAVFGLGNKTYEH 180
Query: 607 FNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEES 786
FNAMGKYVD+RLEQLGAQRIF FITWREQFWPAVCE FGVEATGEES
Sbjct: 181 FNAMGKYVDQRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEFFGVEATGEES 240
Query: 787 SIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHL 966
SIRQYELVVH DMDTA VYTGEMGRLKSYENQKPPFDAKNPFLA VTTNRKLNQGTERHL
Sbjct: 241 SIRQYELVVHEDMDTAKVYTGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHL 300
Query: 967 MHLELGISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHP 1146
MHLEL ISDSKIRYESGDHVAVYPANDS LVNQ+GEILG DLD++MSLNNLDEES ++HP
Sbjct: 301 MHLELDISDSKIRYESGDHVAVYPANDSTLVNQIGEILGADLDVIMSLNNLDEESNKKHP 360
Query: 1147 FPCPTTYRTALTYYLDIHQPPRT 1215
FPCPTTYRTALTYYLDI PPRT
Sbjct: 361 FPCPTTYRTALTYYLDITNPPRT 383
>ref|NP_001075945.1| NADPH-dependent diflavin oxidoreductase 1 [Mus musculus].
Length = 598
Score = 102 bits (255), Expect = 5e-22
Identities = 86/313 (27%), Positives = 138/313 (44%), Gaps = 10/313 (3%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDA--HRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
++V +GSQTGTA++ A RL ++A R G R A D Y +++L I L +F
Sbjct: 6 LLVLFGSQTGTAQDEAERLGREARRRRLGCRVQALD--SYSVANL-----IREPLVIFVC 58
Query: 478 ATYGEGDPTDNAQDFYDWLQEADV---DLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
AT G+GDP DN ++F+ ++ + L + +AV GLG+ +Y FN + K + +RL Q
Sbjct: 59 ATTGQGDPPDNMKNFWRFIFRKSLPSSSLCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQ 118
Query: 649 LGAQRIF--XXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEE--SSIRQYELVVH 816
LG + W W + + V E + +
Sbjct: 119 LGGSALLPPCLGDDQHELGPDAAIDPWVGDLWEKIMVMYPVPLDIPEIPHGVPLPSKFIF 178
Query: 817 TDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLELGISD 993
+ E + S Q PP + + PFLA V TN+++ + + +E I+D
Sbjct: 179 QFLQEVPSIGAEELNIASSAPQTPPSELQ-PFLAPVITNQRVTGPQHFQDVRLIEFDITD 237
Query: 994 SKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPTTYRT 1173
S I + +GD V + P+N A Q ++L D + +L + P P T
Sbjct: 238 SNISFAAGDVVFILPSNSEAHTQQFCQVLCLDPNQFFTLKPREPGVPDPPGLPQPCTVWN 297
Query: 1174 ALTYYLDIHQPPR 1212
++ YLDI PR
Sbjct: 298 LVSQYLDIASVPR 310
>ref|NP_001015876.1| tRNA wybutosine-synthesizing protein 1 homolog isoform 1 [Mus
musculus].
Length = 721
Score = 89.7 bits (221), Expect = 4e-18
Identities = 61/153 (39%), Positives = 84/153 (54%), Gaps = 10/153 (6%)
Frame = +1
Query: 238 ETTTSSVKDSSFVEKMKKTG-RNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEE 414
E DSS + K+T + +FYGSQTGTA+ FA L+K + + +E
Sbjct: 48 EKRVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKE 107
Query: 415 YDLSDLSSLPEI-ENALAVFCMATYGEGDPTDNAQDFYDWLQEADVD-------LSGVKY 570
YD D S + EI + F +ATY +G PT++A+ F WL+E+ D L G++Y
Sbjct: 108 YDPDD-SLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRY 166
Query: 571 AVFGLGNKTY-EHFNAMGKYVDKRLEQLGAQRI 666
AVFGLG+ Y HFN + VDK L LGAQR+
Sbjct: 167 AVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRV 199
>ref|NP_849228.1| tRNA wybutosine-synthesizing protein 1 homolog isoform 2 [Mus
musculus].
Length = 454
Score = 89.7 bits (221), Expect = 4e-18
Identities = 61/153 (39%), Positives = 84/153 (54%), Gaps = 10/153 (6%)
Frame = +1
Query: 238 ETTTSSVKDSSFVEKMKKTG-RNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEE 414
E DSS + K+T + +FYGSQTGTA+ FA L+K + + +E
Sbjct: 48 EKRVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKE 107
Query: 415 YDLSDLSSLPEI-ENALAVFCMATYGEGDPTDNAQDFYDWLQEADVD-------LSGVKY 570
YD D S + EI + F +ATY +G PT++A+ F WL+E+ D L G++Y
Sbjct: 108 YDPDD-SLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRY 166
Query: 571 AVFGLGNKTY-EHFNAMGKYVDKRLEQLGAQRI 666
AVFGLG+ Y HFN + VDK L LGAQR+
Sbjct: 167 AVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRV 199
>ref|NP_032738.1| nitric oxide synthase, brain [Mus musculus].
Length = 1429
Score = 84.7 bits (208), Expect = 1e-16
Identities = 90/378 (23%), Positives = 143/378 (37%), Gaps = 78/378 (20%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYG 489
+ Y ++TG ++ +A L + ++ A EEYD+ L AL + +T+G
Sbjct: 757 ILYATETGKSQAYAKTLC-EIFKHAFDAKAMSMEEYDIVHLE-----HEALVLVVTSTFG 810
Query: 490 EGDPTDNAQDFYDWLQE------------------------------------------A 543
GDP +N + F L E +
Sbjct: 811 NGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFES 870
Query: 544 DVDLSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITW 723
L+ V+++VFGLG++ Y HF A G VD LE+LG +RI F TW
Sbjct: 871 TGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTW 930
Query: 724 REQFWPAVCEHFGVEATGEESSI-RQYELVVHTDMD--------TAVVYTGEM------- 855
++ + A C+ F V G++ +I + ++ D T V E+
Sbjct: 931 AKKVFKAACDVFCV---GDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNV 987
Query: 856 -------GRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTERHLMHLELGISDSKIRYES 1014
RL S +N + P +++ + TN + +++Y+
Sbjct: 988 HKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTN------------------GNQELQYQP 1029
Query: 1015 GDHVAVYPANDSALVNQLGEIL--GTDLDIVMSLNNLDE-----------ESXQRHPFPC 1155
GDH+ V+P N LVN L E L + V+ + L+E + R P
Sbjct: 1030 GDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLP--- 1086
Query: 1156 PTTYRTALTYYLDIHQPP 1209
P T A YYLDI PP
Sbjct: 1087 PCTIFQAFKYYLDITTPP 1104
>ref|NP_032739.3| nitric oxide synthase, endothelial [Mus musculus].
Length = 1202
Score = 84.0 bits (206), Expect = 2e-16
Identities = 101/412 (24%), Positives = 162/412 (39%), Gaps = 73/412 (17%)
Frame = +1
Query: 193 IFRKKK-DEVPEFTKIETTTSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKD 369
I RKK EV KI ++S+ + +++K T + YGS+TG A+ +A +L +
Sbjct: 489 ITRKKTFKEVANAVKI---SASLMGTVMAKRVKAT-----ILYGSETGRAQSYAQQLGRL 540
Query: 370 AHRYGMRGMAADP-----EEYDLSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWL 534
+ A DP +EYD+ L AL + +T+G GDP +N + F L
Sbjct: 541 FRK------AFDPRVLCMDEYDVVSLE-----HEALVLVVTSTFGNGDPPENGESFAAAL 589
Query: 535 QE-----------------------------------------ADVDLSG----VKYAVF 579
E ++ D +G +++ VF
Sbjct: 590 MEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVF 649
Query: 580 GLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHF 759
GLG++ Y HF A + VD RLE+LG +R+ F W + + A CE F
Sbjct: 650 GLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETF 709
Query: 760 GV---------EATGEESSIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPF 912
V + + S ++ + T ++ + G ++ +++ F A
Sbjct: 710 CVGEDAKAAARDIFSPKRSWKRQRYRLSTQAESLQLLPG-----LTHVHRRKMFQA---- 760
Query: 913 LAVVTTNRKLNQGTERH-LMHLELGISDSKIRYESGDHVAVYPANDSALVNQLGEILGTD 1089
+ N + ++ T L+ L+ G ++Y+ GDH+ V P N LV L L
Sbjct: 761 TILSVENLQSSKSTRATILVRLDTG-GQEGLQYQPGDHIGVCPPNRPGLVEAL---LSRV 816
Query: 1090 LDIVMSLNNLDEESXQ------------RHPFPCPTTYRTALTYYLDIHQPP 1209
D S + E + R P P T R ALTY+LDI PP
Sbjct: 817 EDPPPSTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTYFLDITSPP 868
>ref|NP_766068.1| methionine synthase reductase [Mus musculus].
Length = 696
Score = 73.9 bits (180), Expect = 2e-13
Identities = 45/160 (28%), Positives = 74/160 (46%), Gaps = 6/160 (3%)
Frame = +1
Query: 298 RNIIVFYGSQTGTAEEFANRLSKDAHRYGMRG---MAADPEEYDLSDLSSLPEIENALAV 468
R ++ Y +Q G A+ A +S+ A +G ++ E+YDL + E V
Sbjct: 2 RRFLLLYATQRGQAKAIAEEISEQAVSHGFSADLHCISESEKYDL-------KTETGPLV 54
Query: 469 FCMATYGEGDPTDNAQDFYDWLQEADVD---LSGVKYAVFGLGNKTYEHFNAMGKYVDKR 639
++T G GDP D A+ F + + + ++Y + GLG+ Y +F GK +DKR
Sbjct: 55 MVVSTTGTGDPPDTARKFVKEIHNKTLPTDYFAHLQYGLLGLGDSEYTYFCNGGKVIDKR 114
Query: 640 LEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHF 759
L++LGAQR + W + W A+ +HF
Sbjct: 115 LQELGAQRFYDTGHADDCVGLELVVEPWIDGLWAALTKHF 154
>ref|NP_035057.1| nitric oxide synthase, inducible [Mus musculus].
Length = 1144
Score = 73.6 bits (179), Expect = 3e-13
Identities = 85/341 (24%), Positives = 131/341 (38%), Gaps = 26/341 (7%)
Frame = +1
Query: 265 SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLP 444
S + K+ + V + ++TG +E A L+ Y ++Y S L
Sbjct: 520 SMLMRKVMASRVRATVLFATETGKSEALARDLAT-LFSYAFNTKVVCMDQYKASTLE--- 575
Query: 445 EIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKTYEHFNAMGK 624
E L + +T+G GD N Q L +YAVFGLG+ Y F A
Sbjct: 576 --EEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAH 633
Query: 625 YVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGV------------- 765
+D++L LGA ++ F +W Q + A CE F V
Sbjct: 634 DIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFT 693
Query: 766 -EATGEESSIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKL 942
AT E R + D++ A+ AKN F + + + L
Sbjct: 694 SNATWEPQQYRLIQSPEPLDLNRAL----------------SSIHAKNVFTMRLKSQQNL 737
Query: 943 -NQGTERHLMHLELGISDSK-IRYESGDHVAVYPANDSALVNQLGEILGTDLDI-----V 1101
++ + R + ++L S+ Y G+H+ ++P N +ALV IL +D
Sbjct: 738 QSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQ---GILERVVDCPTPHQT 794
Query: 1102 MSLNNLDEE-----SXQRHPFPCPTTYRTALTYYLDIHQPP 1209
+ L LDE +R P P + ALTY+LDI PP
Sbjct: 795 VCLEVLDESGSYWVKDKRLP---PCSLSQALTYFLDITTPP 832
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.317 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 38,490,938
Number of extensions: 1018299
Number of successful extensions: 3872
Number of sequences better than 1.0e-05: 8
Number of HSP's gapped: 3852
Number of HSP's successfully gapped: 9
Length of query: 405
Length of database: 15,617,559
Length adjustment: 104
Effective length of query: 301
Effective length of database: 12,493,815
Effective search space: 3760638315
Effective search space used: 3760638315
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-013356
(1215 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_000932.3| NADPH--cytochrome P450 reductase [Homo sapiens]. 687 0.0
Alignment gi|NP_001137500.1| NADPH-dependent diflavin oxidoreductase 1 is... 106 4e-23
Alignment gi|NP_001137498.1| NADPH-dependent diflavin oxidoreductase 1 is... 106 4e-23
Alignment gi|NP_055249.1| NADPH-dependent diflavin oxidoreductase 1 isofo... 106 4e-23
Alignment gi|NP_001137499.1| NADPH-dependent diflavin oxidoreductase 1 is... 105 1e-22
Alignment gi|NP_060734.2| tRNA wybutosine-synthesizing protein 1 homolog ... 95 1e-19
Alignment gi|NP_000594.2| nitric oxide synthase, endothelial isoform 1 [H... 82 8e-16
Alignment gi|NP_001191143.1| nitric oxide synthase, brain isoform 3 [Homo... 82 1e-15
Alignment gi|NP_001191142.1| nitric oxide synthase, brain isoform 3 [Homo... 82 1e-15
Alignment gi|NP_000611.1| nitric oxide synthase, brain isoform 1 [Homo sa... 82 1e-15
>ref|NP_000932.3| NADPH--cytochrome P450 reductase [Homo sapiens].
Length = 680
Score = 687 bits (1774), Expect = 0.0
Identities = 335/386 (86%), Positives = 355/386 (91%)
Frame = +1
Query: 58 VISMGDSNVDAGTTTSEMVAEEVSLFSATDMVLFSLIVGLLTYWFIFRKKKDEVPEFTKI 237
+I+MGDS+VD +T SE VAEEVSLFS TDM+LFSLIVGLLTYWF+FRKKK+EVPEFTKI
Sbjct: 1 MINMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKI 60
Query: 238 ETTTSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEY 417
+T TSSV++SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGM+ADPEEY
Sbjct: 61 QTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEY 120
Query: 418 DLSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWLQEADVDLSGVKYAVFGLGNKT 597
DL+DLSSLPEI+NAL VFCMATYGEGDPTDNAQDFYDWLQE DVDLSGVK+AVFGLGNKT
Sbjct: 121 DLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKT 180
Query: 598 YEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATG 777
YEHFNAMGKYVDKRLEQLGAQRIF FITWREQFWPAVCEHFGVEATG
Sbjct: 181 YEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATG 240
Query: 778 EESSIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE 957
EESSIRQYELVVHTD+D A VY GEMGRLKSYENQKPPFDAKNPFLA VTTNRKLNQGTE
Sbjct: 241 EESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTE 300
Query: 958 RHLMHLELGISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQ 1137
RHLMHLEL ISDSKIRYESGDHVAVYPANDSALVNQLG+ILG DLD+VMSLNNLDEES +
Sbjct: 301 RHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNK 360
Query: 1138 RHPFPCPTTYRTALTYYLDIHQPPRT 1215
+HPFPCPT+YRTALTYYLDI PPRT
Sbjct: 361 KHPFPCPTSYRTALTYYLDITNPPRT 386
>ref|NP_001137500.1| NADPH-dependent diflavin oxidoreductase 1 isoform c [Homo sapiens].
Length = 590
Score = 106 bits (265), Expect = 4e-23
Identities = 88/317 (27%), Positives = 150/317 (47%), Gaps = 14/317 (4%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDA--HRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
++V +GSQTGTA++ + RL ++A R G R A D Y + +L I L +F
Sbjct: 6 LLVLFGSQTGTAQDVSERLGREARRRRLGCRVQALD--SYPVVNL-----INEPLVIFVC 58
Query: 478 ATYGEGDPTDNAQDFYDWLQEADVD---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
AT G+GDP DN ++F+ ++ ++ L + +AV GLG+ +Y FN + K + +RL Q
Sbjct: 59 ATTGQGDPPDNMKNFWRFIFRKNLPSTALCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQ 118
Query: 649 LGAQRIFXXXXXXXXXXXXXXFIT--WREQFWPAVCEHFG-----VEATGEESSIRQYEL 807
LG + W W V + E ++ L
Sbjct: 119 LGGSALLPVCLGDDQHELGPDAAVDPWLRDLWDRVLGLYPPPPGLTEIPPGVPLPSKFTL 178
Query: 808 VVHTDMDTAVVYTGEMG-RLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLEL 981
+ + + TG G R+ +Q+PP ++K PFLA + +N+++ + + + +E
Sbjct: 179 LFLQEAPS----TGSEGQRVAHPGSQEPPSESK-PFLAPMISNQRVTGPSHFQDVRLIEF 233
Query: 982 GISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPT 1161
I S I + +GD V + P+N +A V + ++LG D D + L + + P P
Sbjct: 234 DILGSGISFAAGDVVLIQPSNSAAHVQRFCQVLGLDPDQLFMLQPREPDVSSPTRLPQPC 293
Query: 1162 TYRTALTYYLDIHQPPR 1212
+ R +++YLDI PR
Sbjct: 294 SMRHLVSHYLDIASVPR 310
>ref|NP_001137498.1| NADPH-dependent diflavin oxidoreductase 1 isoform a [Homo sapiens].
Length = 606
Score = 106 bits (265), Expect = 4e-23
Identities = 88/317 (27%), Positives = 150/317 (47%), Gaps = 14/317 (4%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDA--HRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
++V +GSQTGTA++ + RL ++A R G R A D Y + +L I L +F
Sbjct: 6 LLVLFGSQTGTAQDVSERLGREARRRRLGCRVQALD--SYPVVNL-----INEPLVIFVC 58
Query: 478 ATYGEGDPTDNAQDFYDWLQEADVD---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
AT G+GDP DN ++F+ ++ ++ L + +AV GLG+ +Y FN + K + +RL Q
Sbjct: 59 ATTGQGDPPDNMKNFWRFIFRKNLPSTALCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQ 118
Query: 649 LGAQRIFXXXXXXXXXXXXXXFIT--WREQFWPAVCEHFG-----VEATGEESSIRQYEL 807
LG + W W V + E ++ L
Sbjct: 119 LGGSALLPVCLGDDQHELGPDAAVDPWLRDLWDRVLGLYPPPPGLTEIPPGVPLPSKFTL 178
Query: 808 VVHTDMDTAVVYTGEMG-RLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLEL 981
+ + + TG G R+ +Q+PP ++K PFLA + +N+++ + + + +E
Sbjct: 179 LFLQEAPS----TGSEGQRVAHPGSQEPPSESK-PFLAPMISNQRVTGPSHFQDVRLIEF 233
Query: 982 GISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPT 1161
I S I + +GD V + P+N +A V + ++LG D D + L + + P P
Sbjct: 234 DILGSGISFAAGDVVLIQPSNSAAHVQRFCQVLGLDPDQLFMLQPREPDVSSPTRLPQPC 293
Query: 1162 TYRTALTYYLDIHQPPR 1212
+ R +++YLDI PR
Sbjct: 294 SMRHLVSHYLDIASVPR 310
>ref|NP_055249.1| NADPH-dependent diflavin oxidoreductase 1 isoform b [Homo sapiens].
Length = 597
Score = 106 bits (265), Expect = 4e-23
Identities = 88/317 (27%), Positives = 150/317 (47%), Gaps = 14/317 (4%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDA--HRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
++V +GSQTGTA++ + RL ++A R G R A D Y + +L I L +F
Sbjct: 6 LLVLFGSQTGTAQDVSERLGREARRRRLGCRVQALD--SYPVVNL-----INEPLVIFVC 58
Query: 478 ATYGEGDPTDNAQDFYDWLQEADVD---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
AT G+GDP DN ++F+ ++ ++ L + +AV GLG+ +Y FN + K + +RL Q
Sbjct: 59 ATTGQGDPPDNMKNFWRFIFRKNLPSTALCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQ 118
Query: 649 LGAQRIFXXXXXXXXXXXXXXFIT--WREQFWPAVCEHFG-----VEATGEESSIRQYEL 807
LG + W W V + E ++ L
Sbjct: 119 LGGSALLPVCLGDDQHELGPDAAVDPWLRDLWDRVLGLYPPPPGLTEIPPGVPLPSKFTL 178
Query: 808 VVHTDMDTAVVYTGEMG-RLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLEL 981
+ + + TG G R+ +Q+PP ++K PFLA + +N+++ + + + +E
Sbjct: 179 LFLQEAPS----TGSEGQRVAHPGSQEPPSESK-PFLAPMISNQRVTGPSHFQDVRLIEF 233
Query: 982 GISDSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPT 1161
I S I + +GD V + P+N +A V + ++LG D D + L + + P P
Sbjct: 234 DILGSGISFAAGDVVLIQPSNSAAHVQRFCQVLGLDPDQLFMLQPREPDVSSPTRLPQPC 293
Query: 1162 TYRTALTYYLDIHQPPR 1212
+ R +++YLDI PR
Sbjct: 294 SMRHLVSHYLDIASVPR 310
>ref|NP_001137499.1| NADPH-dependent diflavin oxidoreductase 1 isoform d [Homo sapiens].
Length = 521
Score = 105 bits (261), Expect = 1e-22
Identities = 89/314 (28%), Positives = 151/314 (48%), Gaps = 11/314 (3%)
Frame = +1
Query: 304 IIVFYGSQTGTAEEFANRLSKDA--HRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCM 477
++V +GSQTGTA++ + RL ++A R G R A D Y + +L I L +F
Sbjct: 6 LLVLFGSQTGTAQDVSERLGREARRRRLGCRVQALD--SYPVVNL-----INEPLVIFVC 58
Query: 478 ATYGEGDPTDNAQDFYDWLQEADVD---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQ 648
AT G+GDP DN ++F+ ++ ++ L + +AV GLG+ +Y FN + K + +RL Q
Sbjct: 59 ATTGQGDPPDNMKNFWRFIFRKNLPSTALCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQ 118
Query: 649 LGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTDMD 828
LG + VC G++ Q+EL + +
Sbjct: 119 LGGSALL------------------------PVC-------LGDD----QHELGLPSKFT 143
Query: 829 TAVVY----TGEMG-RLKSYENQKPPFDAKNPFLAVVTTNRKLNQGTE-RHLMHLELGIS 990
+ TG G R+ +Q+PP ++K PFLA + +N+++ + + + +E I
Sbjct: 144 LLFLQEAPSTGSEGQRVAHPGSQEPPSESK-PFLAPMISNQRVTGPSHFQDVRLIEFDIL 202
Query: 991 DSKIRYESGDHVAVYPANDSALVNQLGEILGTDLDIVMSLNNLDEESXQRHPFPCPTTYR 1170
S I + +GD V + P+N +A V + ++LG D D + L + + P P + R
Sbjct: 203 GSGISFAAGDVVLIQPSNSAAHVQRFCQVLGLDPDQLFMLQPREPDVSSPTRLPQPCSMR 262
Query: 1171 TALTYYLDIHQPPR 1212
+++YLDI PR
Sbjct: 263 HLVSHYLDIASVPR 276
>ref|NP_060734.2| tRNA wybutosine-synthesizing protein 1 homolog [Homo sapiens].
Length = 732
Score = 95.1 bits (235), Expect = 1e-19
Identities = 55/127 (43%), Positives = 74/127 (58%), Gaps = 8/127 (6%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYG 489
+FYGSQTGTA+ FA L++ + + +EYD D + VF +ATY
Sbjct: 81 IFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYT 140
Query: 490 EGDPTDNAQDFYDWLQEADVD-------LSGVKYAVFGLGNKTY-EHFNAMGKYVDKRLE 645
+G PT++A+ F WL+EA +D L G++YAVFGLGN Y HFN +GK VDK L
Sbjct: 141 DGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLW 200
Query: 646 QLGAQRI 666
LGA R+
Sbjct: 201 MLGAHRV 207
>ref|NP_000594.2| nitric oxide synthase, endothelial isoform 1 [Homo sapiens].
Length = 1203
Score = 82.4 bits (202), Expect = 8e-16
Identities = 100/417 (23%), Positives = 162/417 (38%), Gaps = 78/417 (18%)
Frame = +1
Query: 193 IFRKKK-DEVPEFTKIETTTSSVKDSSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKD 369
I RKK EV KI ++S+ + +++K T + YGS+TG A+ +A +L +
Sbjct: 490 ITRKKTFKEVANAVKI---SASLMGTVMAKRVKAT-----ILYGSETGRAQSYAQQLGRL 541
Query: 370 AHRYGMRGMAADP-----EEYDLSDLSSLPEIENALAVFCMATYGEGDPTDNAQDFYDWL 534
+ A DP +EYD+ L L + +T+G GDP +N + F L
Sbjct: 542 FRK------AFDPRVLCMDEYDVVSLE-----HETLVLVVTSTFGNGDPPENGESFAAAL 590
Query: 535 QE-----------------------------------------ADVDLSG----VKYAVF 579
E ++ D +G +++ VF
Sbjct: 591 MEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVF 650
Query: 580 GLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITWREQFWPAVCEHF 759
GLG++ Y HF A + VD RLE+LG +R+ F W + + A CE F
Sbjct: 651 GLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETF 710
Query: 760 GVEATGEESSIRQYELVVHTDMDTAVVYTGEMGRLKSYENQKPPFDAKNPFLAVV----- 924
V GE++ ++ +S++ Q+ A+ L ++
Sbjct: 711 CV---GEDAKAAARDI---------------FSPKRSWKRQRYRLSAQAEGLQLLPGLIH 752
Query: 925 TTNRKLNQGTERHLMHLELGIS-------------DSKIRYESGDHVAVYPANDSALVNQ 1065
RK+ Q T R + +L+ S ++Y+ GDH+ V P N LV
Sbjct: 753 VHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEA 812
Query: 1066 LGEILGTDLDIV--MSLNNLDEESXQ-------RHPFPCPTTYRTALTYYLDIHQPP 1209
L + +++ L++ S R P P T R ALT++LDI PP
Sbjct: 813 LLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPP 869
>ref|NP_001191143.1| nitric oxide synthase, brain isoform 3 [Homo sapiens].
Length = 1098
Score = 82.0 bits (201), Expect = 1e-15
Identities = 87/365 (23%), Positives = 148/365 (40%), Gaps = 65/365 (17%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYG 489
+ Y ++TG ++ +A L + ++ EEYD+ L L + +T+G
Sbjct: 426 ILYATETGKSQAYAKTLC-EIFKHAFDAKVMSMEEYDIVHLE-----HETLVLVVTSTFG 479
Query: 490 EGDPTDNAQDF-----------------------------YDWLQEADVD---------- 552
GDP +N + F Y Q++ D
Sbjct: 480 NGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFES 539
Query: 553 ---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITW 723
L+ V+++VFGLG++ Y HF A G VD LE+LG +RI F TW
Sbjct: 540 AGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTW 599
Query: 724 REQFWPAVCEHFGVEATGEESSI-RQYELVVHTDMD--------TAVVYTGEMGRLKSYE 876
++ + A C+ F V G++ +I + ++ D T V E+ + S
Sbjct: 600 AKKVFKAACDVFCV---GDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNV 656
Query: 877 NQKPPFDAKNPFLAVVTTNRKLNQGTERHLMHLELGISDS-KIRYESGDHVAVYPANDSA 1053
++K A+ +++ + + R + + L + S +++Y+ GDH+ V+P N
Sbjct: 657 HKKRVSAAR-----LLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHED 711
Query: 1054 LVNQLGEIL--GTDLDIVMSLNNLDEESXQ-----------RHPFPCPTTYRTALTYYLD 1194
LVN L E L ++ ++ + L+E + R P P T A YYLD
Sbjct: 712 LVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLP---PCTIFQAFKYYLD 768
Query: 1195 IHQPP 1209
I PP
Sbjct: 769 ITTPP 773
>ref|NP_001191142.1| nitric oxide synthase, brain isoform 3 [Homo sapiens].
Length = 1098
Score = 82.0 bits (201), Expect = 1e-15
Identities = 87/365 (23%), Positives = 148/365 (40%), Gaps = 65/365 (17%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYG 489
+ Y ++TG ++ +A L + ++ EEYD+ L L + +T+G
Sbjct: 426 ILYATETGKSQAYAKTLC-EIFKHAFDAKVMSMEEYDIVHLE-----HETLVLVVTSTFG 479
Query: 490 EGDPTDNAQDF-----------------------------YDWLQEADVD---------- 552
GDP +N + F Y Q++ D
Sbjct: 480 NGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFES 539
Query: 553 ---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITW 723
L+ V+++VFGLG++ Y HF A G VD LE+LG +RI F TW
Sbjct: 540 AGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTW 599
Query: 724 REQFWPAVCEHFGVEATGEESSI-RQYELVVHTDMD--------TAVVYTGEMGRLKSYE 876
++ + A C+ F V G++ +I + ++ D T V E+ + S
Sbjct: 600 AKKVFKAACDVFCV---GDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNV 656
Query: 877 NQKPPFDAKNPFLAVVTTNRKLNQGTERHLMHLELGISDS-KIRYESGDHVAVYPANDSA 1053
++K A+ +++ + + R + + L + S +++Y+ GDH+ V+P N
Sbjct: 657 HKKRVSAAR-----LLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHED 711
Query: 1054 LVNQLGEIL--GTDLDIVMSLNNLDEESXQ-----------RHPFPCPTTYRTALTYYLD 1194
LVN L E L ++ ++ + L+E + R P P T A YYLD
Sbjct: 712 LVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLP---PCTIFQAFKYYLD 768
Query: 1195 IHQPP 1209
I PP
Sbjct: 769 ITTPP 773
>ref|NP_000611.1| nitric oxide synthase, brain isoform 1 [Homo sapiens].
Length = 1434
Score = 82.0 bits (201), Expect = 1e-15
Identities = 87/365 (23%), Positives = 148/365 (40%), Gaps = 65/365 (17%)
Frame = +1
Query: 310 VFYGSQTGTAEEFANRLSKDAHRYGMRGMAADPEEYDLSDLSSLPEIENALAVFCMATYG 489
+ Y ++TG ++ +A L + ++ EEYD+ L L + +T+G
Sbjct: 762 ILYATETGKSQAYAKTLC-EIFKHAFDAKVMSMEEYDIVHLE-----HETLVLVVTSTFG 815
Query: 490 EGDPTDNAQDF-----------------------------YDWLQEADVD---------- 552
GDP +N + F Y Q++ D
Sbjct: 816 NGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFES 875
Query: 553 ---LSGVKYAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFXXXXXXXXXXXXXXFITW 723
L+ V+++VFGLG++ Y HF A G VD LE+LG +RI F TW
Sbjct: 876 AGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTW 935
Query: 724 REQFWPAVCEHFGVEATGEESSI-RQYELVVHTDMD--------TAVVYTGEMGRLKSYE 876
++ + A C+ F V G++ +I + ++ D T V E+ + S
Sbjct: 936 AKKVFKAACDVFCV---GDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNV 992
Query: 877 NQKPPFDAKNPFLAVVTTNRKLNQGTERHLMHLELGISDS-KIRYESGDHVAVYPANDSA 1053
++K A+ +++ + + R + + L + S +++Y+ GDH+ V+P N
Sbjct: 993 HKKRVSAAR-----LLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHED 1047
Query: 1054 LVNQLGEIL--GTDLDIVMSLNNLDEESXQ-----------RHPFPCPTTYRTALTYYLD 1194
LVN L E L ++ ++ + L+E + R P P T A YYLD
Sbjct: 1048 LVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLP---PCTIFQAFKYYLD 1104
Query: 1195 IHQPP 1209
I PP
Sbjct: 1105 ITTPP 1109
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.317 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 45,645,887
Number of extensions: 1233242
Number of successful extensions: 4864
Number of sequences better than 1.0e-05: 15
Number of HSP's gapped: 4830
Number of HSP's successfully gapped: 19
Length of query: 405
Length of database: 18,297,164
Length adjustment: 105
Effective length of query: 300
Effective length of database: 14,835,944
Effective search space: 4450783200
Effective search space used: 4450783200
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601C-013356
(1215 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GL894019.2| Sus scrofa unplaced genomic scaffold ChrUScaf1904 303 1e-79
>gb|GL894019.2| Sus scrofa unplaced genomic scaffold ChrUScaf1904
Length = 181737
Score = 303 bits (153), Expect = 1e-79
Identities = 153/153 (100%)
Strand = Plus / Plus
Query: 423 gtctgacctgagcagcctgccagagatcgagaacgccctggcagtgttctgcatggccac 482
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 48846 gtctgacctgagcagcctgccagagatcgagaacgccctggcagtgttctgcatggccac 48905
Query: 483 ctacggcgagggggaccccactgacaatgcccaggacttctacgactggctgcaggaggc 542
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 48906 ctacggcgagggggaccccactgacaatgcccaggacttctacgactggctgcaggaggc 48965
Query: 543 agacgtggacctctctggagtcaagtatgcggt 575
|||||||||||||||||||||||||||||||||
Sbjct: 48966 agacgtggacctctctggagtcaagtatgcggt 48998
Score = 250 bits (126), Expect = 2e-63
Identities = 126/126 (100%)
Strand = Plus / Plus
Query: 121 gaagtgtcccttttcagcgccacagacatggtcctgttttctctcatcgtgggtctcctg 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 29145 gaagtgtcccttttcagcgccacagacatggtcctgttttctctcatcgtgggtctcctg 29204
Query: 181 acctactggttcatcttcagaaagaaaaaagacgaagtccccgaattcaccaaaattgag 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 29205 acctactggttcatcttcagaaagaaaaaagacgaagtccccgaattcaccaaaattgag 29264
Query: 241 accacg 246
||||||
Sbjct: 29265 accacg 29270
Score = 250 bits (126), Expect = 2e-63
Identities = 126/126 (100%)
Strand = Plus / Plus
Query: 573 ggtattcggccttgggaacaagacctacgagcacttcaatgccatgggcaagtatgtgga 632
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 49426 ggtattcggccttgggaacaagacctacgagcacttcaatgccatgggcaagtatgtgga 49485
Query: 633 caagcggctggagcagctcggggcccagcggatctttgatctggggctgggcgacgacga 692
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 49486 caagcggctggagcagctcggggcccagcggatctttgatctggggctgggcgacgacga 49545
Query: 693 cggcaa 698
||||||
Sbjct: 49546 cggcaa 49551
Score = 230 bits (116), Expect = 1e-57
Identities = 119/120 (99%)
Strand = Plus / Plus
Query: 888 cccctttgacgccaagaacccgttcttggctgtcgtcaccaccaaccggaagctgaacca 947
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51490 cccctttgacgccaagaacccgttcttggctgtcgtcaccaccaaccggaagctgaacca 51549
Query: 948 ggggaccgagcgacacctcatgcacctggaactgggcatctcagattccaaaatcaggta 1007
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct: 51550 ggggaccgagcgacacctcatgcacctggaactggacatctcagattccaaaatcaggta 51609
Score = 141 bits (71), Expect = 1e-30
Identities = 90/94 (95%), Gaps = 2/94 (2%)
Strand = Plus / Plus
Query: 1123 gaggagtccaancagaggcacccgttcccctgccccaccacctaccgcacggccctcacc 1182
||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 53914 gaggagtccaacaagaggcacccgttcccctgccccaccacctaccgcacggccctcacc 53973
Query: 1183 tactacctggacattcaccaa-ccgccgcgcacc 1215
||||||||||||| ||||||| ||||||||||||
Sbjct: 53974 tactacctggaca-tcaccaacccgccgcgcacc 54006
Score = 137 bits (69), Expect = 2e-29
Identities = 69/69 (100%)
Strand = Plus / Plus
Query: 1055 tggtcaaccaacttggcgagatcctgggcaccgatctggatatcgtcatgtccctgaaca 1114
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51811 tggtcaaccaacttggcgagatcctgggcaccgatctggatatcgtcatgtccctgaaca 51870
Query: 1115 atcttgacg 1123
|||||||||
Sbjct: 51871 atcttgacg 51879
Score = 121 bits (61), Expect = 9e-25
Identities = 61/61 (100%)
Strand = Plus / Plus
Query: 363 caaggacgcccaccgctacgggatgcggggcatggccgcggaccccgaggagtatgacct 422
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47268 caaggacgcccaccgctacgggatgcggggcatggccgcggaccccgaggagtatgacct 47327
Query: 423 g 423
|
Sbjct: 47328 g 47328
Score = 109 bits (55), Expect = 4e-21
Identities = 55/55 (100%)
Strand = Plus / Plus
Query: 294 gggcaggaacatcatcgtgttctacggctcccagacggggacggcggaggagttc 348
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 47113 gggcaggaacatcatcgtgttctacggctcccagacggggacggcggaggagttc 47167
Score = 109 bits (55), Expect = 4e-21
Identities = 55/55 (100%)
Strand = Plus / Plus
Query: 833 ccgtggtgtacacgggcgagatggggcgcctgaagagctatgagaaccagaagcc 887
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 50534 ccgtggtgtacacgggcgagatggggcgcctgaagagctatgagaaccagaagcc 50588
Score = 99.6 bits (50), Expect = 3e-18
Identities = 50/50 (100%)
Strand = Plus / Plus
Query: 246 gacctcctccgtcaaagacagcagcttcgtggaaaagatgaagaagacgg 295
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 44213 gacctcctccgtcaaagacagcagcttcgtggaaaagatgaagaagacgg 44262
Score = 91.7 bits (46), Expect = 8e-16
Identities = 46/46 (100%)
Strand = Plus / Plus
Query: 699 cctcgaggaggacttcatcacgtggcgagagcagttctggccagcc 744
||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 49933 cctcgaggaggacttcatcacgtggcgagagcagttctggccagcc 49978
Score = 83.8 bits (42), Expect = 2e-13
Identities = 45/46 (97%)
Strand = Plus / Plus
Query: 53 gtttcgtgatcagcatgggggactccaacgtggatgctggcaccac 98
||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 28999 gtttcgtgatcagcatgggggactccaacgtggatactggcaccac 29044
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 27,491,903
Number of extensions: 118
Number of successful extensions: 118
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 118
Number of HSP's successfully gapped: 12
Length of query: 1215
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 1194
Effective length of database: 2,808,413,156
Effective search space: 3353245308264
Effective search space used: 3353245308264
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)