Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601S-063956
(1196 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001029772.1| D-beta-hydroxybutyrate dehydrogenase, mitoch... 308 6e-84
Alignment gi|NP_001069194.1| estradiol 17-beta-dehydrogenase 2 [Bos tauru... 105 1e-22
Alignment gi|NP_001030472.1| 17-beta-hydroxysteroid dehydrogenase type 6 ... 100 3e-21
Alignment gi|NP_777067.1| corticosteroid 11-beta-dehydrogenase isozyme 2 ... 94 2e-19
Alignment gi|NP_001069264.1| retinol dehydrogenase 16 [Bos taurus]. 92 1e-18
Alignment gi|NP_777158.1| dehydrogenase/reductase SDR family member 9 pre... 88 1e-17
Alignment gi|NP_001077206.1| short-chain dehydrogenase/reductase family 9... 81 2e-15
Alignment gi|NP_776591.2| 11-cis retinol dehydrogenase [Bos taurus]. 76 5e-14
Alignment gi|NP_776592.1| retinol dehydrogenase 8 [Bos taurus]. 52 8e-07
>ref|NP_001029772.1| D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor [Bos
taurus].
Length = 344
Score = 308 bits (789), Expect = 6e-84
Identities = 155/183 (84%), Positives = 162/183 (88%)
Frame = +2
Query: 236 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 415
RLSRPLSQLPRKTLNFS+RENG RGSLL YS FVP RRTYA SV+PVGSKAVL+TGCD
Sbjct: 5 RLSRPLSQLPRKTLNFSDRENGTRGSLLLYSAPFVPVGRRTYAASVDPVGSKAVLITGCD 64
Query: 416 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXX 595
SGFGFSLAKHLHS+GFLVFAGCLMKDKG DGVKELDS+KSDRLRTVQLN
Sbjct: 65 SGFGFSLAKHLHSEGFLVFAGCLMKDKGSDGVKELDSMKSDRLRTVQLNVCKSEEVDKAA 124
Query: 596 XTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIR 775
IRSSL+DPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTK+FLPLIR
Sbjct: 125 EVIRSSLEDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKAFLPLIR 184
Query: 776 RAK 784
RAK
Sbjct: 185 RAK 187
>ref|NP_001069194.1| estradiol 17-beta-dehydrogenase 2 [Bos taurus].
Length = 388
Score = 105 bits (261), Expect = 1e-22
Identities = 62/137 (45%), Positives = 77/137 (56%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV KAVL+TGCDSGFG LAK+L GF VFAG L D+ G +EL S L +Q
Sbjct: 80 PVDQKAVLITGCDSGFGHGLAKYLDELGFTVFAGVL--DEQGSGAEELRRTCSKNLSVLQ 137
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTF-GDVEFTSMETYKEVAEVNLW 733
LN ++ LQ+ KGLW ++NNAG+ D E M YK VN +
Sbjct: 138 LNITNTQEIKAAYSKVKEKLQN--KGLWAVINNAGVLGLPTDGELIPMTEYKRCMAVNFF 195
Query: 734 GTVRVTKSFLPLIRRAK 784
G V VTK FLPL+R++K
Sbjct: 196 GAVEVTKVFLPLLRKSK 212
>ref|NP_001030472.1| 17-beta-hydroxysteroid dehydrogenase type 6 precursor [Bos taurus].
Length = 317
Score = 100 bits (248), Expect = 3e-21
Identities = 58/139 (41%), Positives = 82/139 (58%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V AGCL + G ++L + SDRL+T
Sbjct: 24 VSHLQDKFVFITGCDSGFGNQLARQLDLRGLRVLAGCLTE----QGAEQLRNQTSDRLQT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVN 727
V L+ ++ + D +GLWGLVNNAGI + G E+ +ETY++ VN
Sbjct: 80 VILDVTKTESIAAATEWVKECVGD--RGLWGLVNNAGIFHSHGYAEWIKIETYRDTLRVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT S LPL+R+A+
Sbjct: 138 LIGVIEVTLSMLPLVRKAQ 156
>ref|NP_777067.1| corticosteroid 11-beta-dehydrogenase isozyme 2 [Bos taurus].
Length = 404
Score = 94.0 bits (232), Expect = 2e-19
Identities = 57/145 (39%), Positives = 79/145 (54%), Gaps = 1/145 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV ++AVL+TGCDSGFG + AK L + GF V A L D G EL + S RL+ +Q
Sbjct: 79 PVATRAVLITGCDSGFGNATAKKLDTMGFTVLATVL--DLNSPGALELRACCSSRLKLLQ 136
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTF-GDVEFTSMETYKEVAEVNLW 733
++ + + P GLWGLVNNAG + F D E + T++ EVN +
Sbjct: 137 MDLTKPGDISRVLEF--TKVHTPSTGLWGLVNNAGQNIFVADAELCPVATFRTCMEVNFF 194
Query: 734 GTVRVTKSFLPLIRRAKDFQETTTS 808
G + +TK LPL+RR+ T +S
Sbjct: 195 GALEMTKGLLPLLRRSSGRIVTVSS 219
>ref|NP_001069264.1| retinol dehydrogenase 16 [Bos taurus].
Length = 317
Score = 91.7 bits (226), Expect = 1e-18
Identities = 55/139 (39%), Positives = 81/139 (58%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L + SDRL+T
Sbjct: 24 VSHLRDKFVFITGCDSGFGNLLARQLDLRGLRVLAACLTE----KGAEQLRNQTSDRLQT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIST-FGDVEFTSMETYKEVAEVN 727
V L+ ++ + D +GLWGLVNNAGIST E+ + + + ++ +VN
Sbjct: 80 VILDVTKTDSVAAATEWVKERVGD--RGLWGLVNNAGISTPTAPNEWLTKQDFMKILDVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT S LPL+R+A+
Sbjct: 138 LLGVIDVTLSVLPLVRKAR 156
>ref|NP_777158.1| dehydrogenase/reductase SDR family member 9 precursor [Bos taurus].
Length = 319
Score = 88.2 bits (217), Expect = 1e-17
Identities = 51/136 (37%), Positives = 76/136 (55%), Gaps = 1/136 (0%)
Frame = +2
Query: 380 VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQL 559
+ K + +TGCD+GFG A+ KGF V A CL + G L + S+RL TV L
Sbjct: 27 ITDKYIFITGCDTGFGNLAARTFDKKGFHVIAACLTE----SGSTALKAETSERLHTVLL 82
Query: 560 NXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVNLWG 736
+ +++ Q EKGLWGL+NNAGI ++ ++E Y+E EVNL+G
Sbjct: 83 DVTDPENVKRAAQWVKN--QVGEKGLWGLINNAGILGVLAPNDWLTVEDYREPVEVNLFG 140
Query: 737 TVRVTKSFLPLIRRAK 784
+ VT + LPL+++A+
Sbjct: 141 LISVTLNMLPLVKKAR 156
>ref|NP_001077206.1| short-chain dehydrogenase/reductase family 9C member 7 [Bos
taurus].
Length = 313
Score = 81.3 bits (199), Expect = 2e-15
Identities = 50/139 (35%), Positives = 74/139 (53%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V + K V +TGCDSGFG LA+ L +G V A C K +G ++L S +L+T
Sbjct: 20 VRNLSDKYVFITGCDSGFGNLLARQLVDRGMRVLAACFTK----EGAQKLQQDTSYQLQT 75
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVN 727
+ L+ +R Q E+GLW LVNNAG+ G E+ + E + +V VN
Sbjct: 76 ILLDVTKTESIKAATQWVRD--QVGEQGLWALVNNAGVGLPSGPNEWLTKEDFVKVINVN 133
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT LP++++A+
Sbjct: 134 LVGLIEVTLHMLPMVKKAR 152
>ref|NP_776591.2| 11-cis retinol dehydrogenase [Bos taurus].
Length = 318
Score = 76.3 bits (186), Expect = 5e-14
Identities = 47/137 (34%), Positives = 69/137 (50%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
P + +TGCDSGFG LA L +GF V A CL G ++L + S RL T
Sbjct: 25 PASDAFIFITGCDSGFGRLLALRLDQRGFRVLASCLTP----SGAEDLQRVASSRLHTTL 80
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVNLW 733
L+ + + + E GL+GLVNNAG++ G + + E ++ V VN
Sbjct: 81 LDVTDPQSIRQAVKWVETHV--GEAGLFGLVNNAGVAGIIGPTPWQTREDFQRVLNVNTL 138
Query: 734 GTVRVTKSFLPLIRRAK 784
G + VT + LPL+ +A+
Sbjct: 139 GPIGVTLALLPLLLQAR 155
>ref|NP_776592.1| retinol dehydrogenase 8 [Bos taurus].
Length = 312
Score = 52.4 bits (124), Expect = 8e-07
Identities = 42/150 (28%), Positives = 62/150 (41%), Gaps = 3/150 (2%)
Frame = +2
Query: 389 KAVLVTGCDSGFGFSLAKHL-HSKGFLVFAGCLMKDKGHDGVKELDSLKS--DRLRTVQL 559
+ VL++GC SG G LA L H M+D G G E + ++ L QL
Sbjct: 6 RTVLISGCSSGIGLELAVQLAHDPRQRYQVVATMRDLGKKGTLETAAGEALGQTLTVAQL 65
Query: 560 NXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGT 739
+ I+ D LVNNAG+ G +E S+ + V + N +G
Sbjct: 66 DVCSDESVAQCLNCIQGGEVDV------LVNNAGVGLVGPLEGLSLAAMQNVFDTNFFGA 119
Query: 740 VRVTKSFLPLIRRAKDFQETTTSKCPSHQG 829
VR+ K+ LP ++R + S QG
Sbjct: 120 VRLVKAVLPSMKRRRQGHIVVVSSVMGLQG 149
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 42,444,069
Number of extensions: 1207208
Number of successful extensions: 3939
Number of sequences better than 1.0e-05: 9
Number of HSP's gapped: 3911
Number of HSP's successfully gapped: 9
Length of query: 398
Length of database: 17,681,374
Length adjustment: 104
Effective length of query: 294
Effective length of database: 14,240,222
Effective search space: 4186625268
Effective search space used: 4186625268
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601S-063956
(1196 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_545160.2| PREDICTED: similar to 3-hydroxybutyrate dehydro... 226 4e-59
Alignment gi|XP_546810.2| PREDICTED: similar to hydroxysteroid (17-beta) ... 100 5e-21
Alignment gi|XP_538239.2| PREDICTED: similar to 3-hydroxysteroid epimeras... 90 4e-18
Alignment gi|XP_858059.1| PREDICTED: similar to 3-hydroxysteroid epimeras... 90 4e-18
Alignment gi|XP_545513.2| PREDICTED: similar to NADP-dependent retinol de... 87 2e-17
Alignment gi|XP_858182.1| PREDICTED: similar to microsomal NAD+-dependent... 87 4e-17
Alignment gi|XP_531641.2| PREDICTED: similar to microsomal NAD+-dependent... 87 4e-17
Alignment gi|XP_538240.2| PREDICTED: similar to orphan short-chain dehydr... 80 3e-15
Alignment gi|XP_538220.2| PREDICTED: similar to 11-cis retinol dehydrogen... 77 3e-14
Alignment gi|XP_853935.1| PREDICTED: similar to Corticosteroid 11-beta-de... 75 9e-14
>ref|XP_545160.2| PREDICTED: similar to 3-hydroxybutyrate dehydrogenase precursor
[Canis familiaris].
Length = 299
Score = 226 bits (575), Expect = 4e-59
Identities = 110/135 (81%), Positives = 120/135 (88%)
Frame = +2
Query: 380 VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQL 559
VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKD+G DGVKELD LKSDRLRT+QL
Sbjct: 9 VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDQGGDGVKELDGLKSDRLRTIQL 68
Query: 560 NXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGT 739
N +RSSL+DPEKG+WGLVNNAGISTFG+VEFTSMETYKEVAEVNLWGT
Sbjct: 69 NVCKGEEVERVVEVVRSSLEDPEKGMWGLVNNAGISTFGEVEFTSMETYKEVAEVNLWGT 128
Query: 740 VRVTKSFLPLIRRAK 784
V++TK+FLPLIRRA+
Sbjct: 129 VQMTKAFLPLIRRAR 143
>ref|XP_546810.2| PREDICTED: similar to hydroxysteroid (17-beta) dehydrogenase 2
[Canis familiaris].
Length = 382
Score = 99.8 bits (247), Expect = 5e-21
Identities = 60/137 (43%), Positives = 78/137 (56%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV KAVL+TG DSGFG +L+K+L GF VF G L D+ G +EL S RL +Q
Sbjct: 80 PVDQKAVLITGGDSGFGHALSKYLDELGFTVFVGVL--DEKGSGAEELRRTCSKRLSVLQ 137
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTF-GDVEFTSMETYKEVAEVNLW 733
++ + LQD +GLW +VNNAGI D E M YK+ VN +
Sbjct: 138 MDITDQQQIKDAHSKVVEKLQD--RGLWAVVNNAGIICLPADGELIPMTDYKKCMAVNFF 195
Query: 734 GTVRVTKSFLPLIRRAK 784
G V VTK+FLPL+R++K
Sbjct: 196 GAVEVTKAFLPLLRKSK 212
>ref|XP_538239.2| PREDICTED: similar to 3-hydroxysteroid epimerase isoform 3 [Canis
familiaris].
Length = 317
Score = 90.1 bits (222), Expect = 4e-18
Identities = 58/139 (41%), Positives = 77/139 (55%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L S SDRL T
Sbjct: 24 VSHLHDKYVFITGCDSGFGNLLARQLDLRGLRVLAACLTE----KGAQQLRSQTSDRLDT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVN 727
V L+ + + D +GLWGLVNNAGI E+ +E Y +V +VN
Sbjct: 80 VILDVTKTESILAATQWVEERVGD--RGLWGLVNNAGIFHPHCYSEWLKIEAYVDVFKVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT S LPL+RRA+
Sbjct: 138 LTGLIEVTLSMLPLLRRAR 156
>ref|XP_858059.1| PREDICTED: similar to 3-hydroxysteroid epimerase isoform 4 [Canis
familiaris].
Length = 323
Score = 90.1 bits (222), Expect = 4e-18
Identities = 58/139 (41%), Positives = 77/139 (55%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L S SDRL T
Sbjct: 24 VSHLHDKYVFITGCDSGFGNLLARQLDLRGLRVLAACLTE----KGAQQLRSQTSDRLDT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVN 727
V L+ + + D +GLWGLVNNAGI E+ +E Y +V +VN
Sbjct: 80 VILDVTKTESILAATQWVEERVGD--RGLWGLVNNAGIFHPHCYSEWLKIEAYVDVFKVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT S LPL+RRA+
Sbjct: 138 LTGLIEVTLSMLPLLRRAR 156
>ref|XP_545513.2| PREDICTED: similar to NADP-dependent retinol
dehydrogenase/reductase [Canis familiaris].
Length = 753
Score = 87.4 bits (215), Expect = 2e-17
Identities = 49/136 (36%), Positives = 76/136 (55%), Gaps = 1/136 (0%)
Frame = +2
Query: 380 VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQL 559
+ K + +TGCD+GFG A+ KGF V A CL + + L + S+RL TV L
Sbjct: 461 ITDKYIFITGCDTGFGNLAARTFDKKGFHVIAACLTESES----TALKAETSERLHTVLL 516
Query: 560 NXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVNLWG 736
+ +++ Q EKGLWGL+NNAG+ ++ ++E Y+E EVNL+G
Sbjct: 517 DVTDPENVKRTAQWVKN--QVGEKGLWGLINNAGVLGVLAPTDWLTVEDYREPIEVNLFG 574
Query: 737 TVRVTKSFLPLIRRAK 784
+ VT + LPL+++A+
Sbjct: 575 LISVTLNLLPLVKKAR 590
>ref|XP_858182.1| PREDICTED: similar to microsomal NAD+-dependent retinol
dehydrogenase 4 isoform 2 [Canis familiaris].
Length = 319
Score = 86.7 bits (213), Expect = 4e-17
Identities = 55/139 (39%), Positives = 77/139 (55%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L S SDRL T
Sbjct: 24 VSHLQDKYVFITGCDSGFGNLLARQLDLRGLRVLAACLTE----KGAQQLRSQTSDRLDT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVN 727
V L+ + + D +GLWGLVNNAGIS E+ + + + ++ +VN
Sbjct: 80 VILDVTKTESILAATQWVEEHVGD--RGLWGLVNNAGISWPTAPNEWLTKDDFMKILDVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G V VT S L L+R+A+
Sbjct: 138 LLGLVEVTLSLLSLVRKAR 156
>ref|XP_531641.2| PREDICTED: similar to microsomal NAD+-dependent retinol
dehydrogenase 4 isoform 1 [Canis familiaris].
Length = 317
Score = 86.7 bits (213), Expect = 4e-17
Identities = 55/139 (39%), Positives = 77/139 (55%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L S SDRL T
Sbjct: 24 VSHLQDKYVFITGCDSGFGNLLARQLDLRGLRVLAACLTE----KGAQQLRSQTSDRLDT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVN 727
V L+ + + D +GLWGLVNNAGIS E+ + + + ++ +VN
Sbjct: 80 VILDVTKTESILAATQWVEEHVGD--RGLWGLVNNAGISWPTAPNEWLTKDDFMKILDVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G V VT S L L+R+A+
Sbjct: 138 LLGLVEVTLSLLSLVRKAR 156
>ref|XP_538240.2| PREDICTED: similar to orphan short-chain dehydrogenase / reductase
[Canis familiaris].
Length = 313
Score = 80.5 bits (197), Expect = 3e-15
Identities = 51/139 (36%), Positives = 74/139 (53%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V + K V +TGCDSGFG LA+ L ++G V A C + +G ++L S RL+T
Sbjct: 20 VRNLSDKYVFITGCDSGFGNLLARQLVNRGMRVLAACFTE----EGAQKLQRDTSYRLQT 75
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVN 727
L+ +R Q E+GLW LVNNAGI G E+ + E + +V VN
Sbjct: 76 TLLDVTRTESIQAATQWVRD--QVGEQGLWALVNNAGIGLPSGPNEWLTKEDFVKVINVN 133
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT LP++++A+
Sbjct: 134 LVGLIEVTLHMLPMVKKAR 152
>ref|XP_538220.2| PREDICTED: similar to 11-cis retinol dehydrogenase (11-cis RDH)
[Canis familiaris].
Length = 339
Score = 77.0 bits (188), Expect = 3e-14
Identities = 47/137 (34%), Positives = 70/137 (51%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
P V +TGCDSGFG LA L +GF V A CL G ++L + S RL T
Sbjct: 46 PASDAFVFITGCDSGFGRLLALRLDQRGFRVLASCLTP----AGAEDLQRVASSRLHTTL 101
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVNLW 733
L+ + + + E GL+GLVNNAG++ G + + + + V VN
Sbjct: 102 LDVTEPQSVQRAAKWVETHV--GEAGLFGLVNNAGVAGIIGPTPWLTQDDFHRVLNVNTL 159
Query: 734 GTVRVTKSFLPLIRRAK 784
G +RVT + LPL+++++
Sbjct: 160 GPIRVTLALLPLLQQSR 176
>ref|XP_853935.1| PREDICTED: similar to Corticosteroid 11-beta-dehydrogenase, isozyme
2 (11-DH2) (11-beta-hydroxysteroid dehydrogenase type 2)
(11-beta-HSD2) (NAD-dependent 11-beta-hydroxysteroid
dehydrogenase) [Canis familiaris].
Length = 323
Score = 75.5 bits (184), Expect = 9e-14
Identities = 48/126 (38%), Positives = 66/126 (52%), Gaps = 1/126 (0%)
Frame = +2
Query: 410 CDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXX 589
CDSGFG AK L + GF V A L D G EL + S RLR +Q++
Sbjct: 8 CDSGFGKETAKKLDAMGFTVLATVLELDG--PGALELRACCSPRLRLLQMDLTKPADISR 65
Query: 590 XXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLP 766
++ GLWGLVNNAG + DVE + + T++ EVN +G + +TK LP
Sbjct: 66 ALEFTKAHTSST--GLWGLVNNAGHNDVVADVELSPVATFRSCMEVNFFGALELTKGLLP 123
Query: 767 LIRRAK 784
L+RR++
Sbjct: 124 LLRRSR 129
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 43,759,683
Number of extensions: 1129992
Number of successful extensions: 4158
Number of sequences better than 1.0e-05: 11
Number of HSP's gapped: 4130
Number of HSP's successfully gapped: 11
Length of query: 398
Length of database: 18,874,504
Length adjustment: 105
Effective length of query: 293
Effective length of database: 15,374,224
Effective search space: 4504647632
Effective search space used: 4504647632
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqSP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601S-063956
(1196 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_003132659.1| PREDICTED: d-beta-hydroxybutyrate dehydrogen... 239 2e-63
Alignment gi|XP_001926007.3| PREDICTED: d-beta-hydroxybutyrate dehydrogen... 173 2e-43
Alignment gi|NP_001161121.1| estradiol 17-beta-dehydrogenase 2 [Sus scrof... 97 1e-20
Alignment gi|NP_999078.1| corticosteroid 11-beta-dehydrogenase isozyme 2 ... 94 1e-19
Alignment gi|XP_003133511.1| PREDICTED: dehydrogenase/reductase SDR famil... 89 4e-18
Alignment gi|XP_003133510.1| PREDICTED: dehydrogenase/reductase SDR famil... 89 4e-18
Alignment gi|XP_003355508.1| PREDICTED: 17-beta-hydroxysteroid dehydrogen... 88 1e-17
Alignment gi|XP_003355507.1| PREDICTED: 17-beta-hydroxysteroid dehydrogen... 88 1e-17
Alignment gi|XP_003360960.1| PREDICTED: estradiol 17-beta-dehydrogenase 2... 87 1e-17
Alignment gi|XP_003355529.1| PREDICTED: retinol dehydrogenase 16-like [Su... 87 1e-17
>ref|XP_003132659.1| PREDICTED: d-beta-hydroxybutyrate dehydrogenase, mitochondrial-like
[Sus scrofa].
Length = 174
Score = 239 bits (610), Expect = 2e-63
Identities = 121/133 (90%), Positives = 121/133 (90%)
Frame = +2
Query: 236 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 415
RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD
Sbjct: 8 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 67
Query: 416 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXX 595
SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLN
Sbjct: 68 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNVCKSEEVEKVV 127
Query: 596 XTIRSSLQDPEKG 634
TIRSSLQDPEKG
Sbjct: 128 ETIRSSLQDPEKG 140
>ref|XP_001926007.3| PREDICTED: d-beta-hydroxybutyrate dehydrogenase, mitochondrial [Sus
scrofa].
Length = 263
Score = 173 bits (438), Expect = 2e-43
Identities = 88/105 (83%), Positives = 90/105 (85%)
Frame = +2
Query: 470 FAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLV 649
F G + +DKGHDGVKELDSLKSDRLRTVQLN TIRSSLQDPEKGLWGLV
Sbjct: 3 FKGIVEEDKGHDGVKELDSLKSDRLRTVQLNVCKSEEVEKVVETIRSSLQDPEKGLWGLV 62
Query: 650 NNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIRRAK 784
NNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIRRAK
Sbjct: 63 NNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIRRAK 107
>ref|NP_001161121.1| estradiol 17-beta-dehydrogenase 2 [Sus scrofa].
Length = 388
Score = 97.4 bits (241), Expect = 1e-20
Identities = 59/137 (43%), Positives = 78/137 (56%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV KAVL+TG DSG G +L+K+L GF+VFAG L D+ G +EL S L +Q
Sbjct: 80 PVDQKAVLITGGDSGIGHALSKYLDKLGFIVFAGVL--DEKGSGAEELRRTCSKHLSVLQ 137
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFG-DVEFTSMETYKEVAEVNLW 733
L+ + +Q+ +GLW +VNNAGI F D E M YK VN +
Sbjct: 138 LDITNSQQIRDAHSKVMEKVQN--RGLWAVVNNAGILGFPIDGELIPMTQYKRCMAVNFF 195
Query: 734 GTVRVTKSFLPLIRRAK 784
G V VTK+FLPL+R++K
Sbjct: 196 GAVEVTKAFLPLLRKSK 212
>ref|NP_999078.1| corticosteroid 11-beta-dehydrogenase isozyme 2 [Sus scrofa].
Length = 400
Score = 94.0 bits (232), Expect = 1e-19
Identities = 57/145 (39%), Positives = 81/145 (55%), Gaps = 1/145 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV ++AVL+TGCDSGFG + AK L + GF V A L D G +EL + S RL+ +Q
Sbjct: 79 PVATRAVLITGCDSGFGKATAKKLDAMGFTVLATVLELDS--PGAQELRACCSPRLKLLQ 136
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTF-GDVEFTSMETYKEVAEVNLW 733
++ + + GLWGLVNNAG + D E + + TY+ EVN +
Sbjct: 137 MDLTKPADISRVLEF--TKVHTASTGLWGLVNNAGHNILVADAELSPVATYRSCMEVNFF 194
Query: 734 GTVRVTKSFLPLIRRAKDFQETTTS 808
GT+ +TK+ LPL+RR+ T +S
Sbjct: 195 GTLEMTKALLPLLRRSSGRIVTVSS 219
>ref|XP_003133511.1| PREDICTED: dehydrogenase/reductase SDR family member 9-like isoform
1 [Sus scrofa].
Length = 319
Score = 89.4 bits (220), Expect = 4e-18
Identities = 51/136 (37%), Positives = 76/136 (55%), Gaps = 1/136 (0%)
Frame = +2
Query: 380 VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQL 559
+ K + +TGCD+GFG A+ KGF V A CL + G L + S+RL TV L
Sbjct: 27 ITDKYIFITGCDTGFGNLAARTFDKKGFHVIAACLTE----SGSTALKAETSERLHTVLL 82
Query: 560 NXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVNLWG 736
+ +++ Q EKGLWGL+NNAGI ++ ++E Y+E EVNL+G
Sbjct: 83 DVTDPENVKRTAQWVKN--QVGEKGLWGLINNAGILGVLAPTDWLTVEDYREPIEVNLFG 140
Query: 737 TVRVTKSFLPLIRRAK 784
+ VT + LPL+++A+
Sbjct: 141 LINVTLNMLPLVKKAR 156
>ref|XP_003133510.1| PREDICTED: dehydrogenase/reductase SDR family member 9-like,
partial [Sus scrofa].
Length = 245
Score = 89.4 bits (220), Expect = 4e-18
Identities = 51/136 (37%), Positives = 76/136 (55%), Gaps = 1/136 (0%)
Frame = +2
Query: 380 VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQL 559
+ K + +TGCD+GFG A+ KGF V A CL + G L + S+RL TV L
Sbjct: 27 ITDKYIFITGCDTGFGNLAARTFDKKGFHVIAACLTE----SGSTALKAETSERLHTVLL 82
Query: 560 NXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVNLWG 736
+ +++ Q EKGLWGL+NNAGI ++ ++E Y+E EVNL+G
Sbjct: 83 DVTDPENVKRTAQWVKN--QVGEKGLWGLINNAGILGVLAPTDWLTVEDYREPIEVNLFG 140
Query: 737 TVRVTKSFLPLIRRAK 784
+ VT + LPL+++A+
Sbjct: 141 LINVTLNMLPLVKKAR 156
>ref|XP_003355508.1| PREDICTED: 17-beta-hydroxysteroid dehydrogenase type 6-like isoform
2 [Sus scrofa].
Length = 337
Score = 87.8 bits (216), Expect = 1e-17
Identities = 56/141 (39%), Positives = 77/141 (54%), Gaps = 3/141 (2%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ +G K V +TGCDSGFG LA+ L +G V A CL + G ++L SDRL T
Sbjct: 44 VSHLGDKYVFITGCDSGFGNLLARQLDVRGLRVLAACLTE----QGAQQLRKQTSDRLET 99
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFGDV---EFTSMETYKEVAE 721
V L+ ++ + D +GLWGLVNNAG TF + E+ ET+ +
Sbjct: 100 VLLDVTKTESIAAATQWVKERVGD--RGLWGLVNNAG--TFHPLCITEWLKTETWIHTLK 155
Query: 722 VNLWGTVRVTKSFLPLIRRAK 784
VNL G VT S LPL+++A+
Sbjct: 156 VNLIGVTEVTLSMLPLVKKAQ 176
>ref|XP_003355507.1| PREDICTED: 17-beta-hydroxysteroid dehydrogenase type 6-like isoform
1 [Sus scrofa].
Length = 317
Score = 87.8 bits (216), Expect = 1e-17
Identities = 56/141 (39%), Positives = 77/141 (54%), Gaps = 3/141 (2%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ +G K V +TGCDSGFG LA+ L +G V A CL + G ++L SDRL T
Sbjct: 24 VSHLGDKYVFITGCDSGFGNLLARQLDVRGLRVLAACLTE----QGAQQLRKQTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFGDV---EFTSMETYKEVAE 721
V L+ ++ + D +GLWGLVNNAG TF + E+ ET+ +
Sbjct: 80 VLLDVTKTESIAAATQWVKERVGD--RGLWGLVNNAG--TFHPLCITEWLKTETWIHTLK 135
Query: 722 VNLWGTVRVTKSFLPLIRRAK 784
VNL G VT S LPL+++A+
Sbjct: 136 VNLIGVTEVTLSMLPLVKKAQ 156
>ref|XP_003360960.1| PREDICTED: estradiol 17-beta-dehydrogenase 2-like, partial [Sus
scrofa].
Length = 212
Score = 87.4 bits (215), Expect = 1e-17
Identities = 59/158 (37%), Positives = 81/158 (51%), Gaps = 1/158 (0%)
Frame = +2
Query: 314 LLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKD 493
L+ + P GS RT+ + + A G DSG G +L+K+L GF VFAG L D
Sbjct: 49 LIGHKPVRGTGSFRTHGPNTPSHANHAGGAQGGDSGIGHALSKYLDKLGFTVFAGVL--D 106
Query: 494 KGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTF 673
+ G +EL S L +QL+ + +Q+ +GLW +VNNAGI F
Sbjct: 107 EKGSGAEELRRTCSKHLSVLQLDITNSQQIRDAHSKVMEKVQN--RGLWAVVNNAGILGF 164
Query: 674 G-DVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIRRAK 784
D E M YK VN +G V VTK+FLPL+R++K
Sbjct: 165 PIDGELIPMTQYKRCMAVNFFGAVEVTKAFLPLLRKSK 202
>ref|XP_003355529.1| PREDICTED: retinol dehydrogenase 16-like [Sus scrofa].
Length = 317
Score = 87.4 bits (215), Expect = 1e-17
Identities = 54/139 (38%), Positives = 75/139 (53%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L SDRL T
Sbjct: 24 VSHLRDKYVFITGCDSGFGNLLARQLDLRGLRVLAACLTE----QGAQQLRKQTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIST-FGDVEFTSMETYKEVAEVN 727
V L+ ++ + D +GLWGLVNNAGI T E+ + + + + +VN
Sbjct: 80 VLLDVTKTESVAAAKEWVKERVGD--RGLWGLVNNAGICTPLAPSEWLTKQDFMVMLDVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G V VT LPL+R+ K
Sbjct: 138 LLGMVDVTLQLLPLVRKTK 156
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 28,469,795
Number of extensions: 949917
Number of successful extensions: 2626
Number of sequences better than 1.0e-05: 18
Number of HSP's gapped: 2593
Number of HSP's successfully gapped: 18
Length of query: 398
Length of database: 11,343,932
Length adjustment: 101
Effective length of query: 297
Effective length of database: 8,829,335
Effective search space: 2622312495
Effective search space used: 2622312495
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 34 (17.7 bits)
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601S-063956
(1196 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_976060.1| D-beta-hydroxybutyrate dehydrogenase, mitochond... 287 2e-77
Alignment gi|NP_976059.1| D-beta-hydroxybutyrate dehydrogenase, mitochond... 287 2e-77
Alignment gi|NP_004042.1| D-beta-hydroxybutyrate dehydrogenase, mitochond... 287 2e-77
Alignment gi|NP_002144.1| estradiol 17-beta-dehydrogenase 2 [Homo sapiens]. 99 1e-20
Alignment gi|NP_001135743.1| dehydrogenase/reductase SDR family member 9 ... 92 7e-19
Alignment gi|NP_954674.1| dehydrogenase/reductase SDR family member 9 pre... 92 7e-19
Alignment gi|NP_001135742.1| dehydrogenase/reductase SDR family member 9 ... 92 7e-19
Alignment gi|NP_005762.2| dehydrogenase/reductase SDR family member 9 pre... 92 7e-19
Alignment gi|NP_003699.3| retinol dehydrogenase 16 [Homo sapiens]. 92 9e-19
Alignment gi|NP_003716.2| 17-beta-hydroxysteroid dehydrogenase type 6 pre... 90 4e-18
>ref|NP_976060.1| D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor [Homo
sapiens].
Length = 343
Score = 287 bits (734), Expect = 2e-77
Identities = 144/183 (78%), Positives = 156/183 (85%)
Frame = +2
Query: 236 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 415
RLSRPLS+LP KTL+ +RENGAR LL S SF+P RRTYA + PVGSKAVLVTGCD
Sbjct: 5 RLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCD 64
Query: 416 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXX 595
SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSL SDRLRTVQLN
Sbjct: 65 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVV 124
Query: 596 XTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIR 775
+RSSL+DPEKG+WGLVNNAGISTFG+VEFTS+ETYK+VAEVNLWGTVR+TKSFLPLIR
Sbjct: 125 EIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIR 184
Query: 776 RAK 784
RAK
Sbjct: 185 RAK 187
>ref|NP_976059.1| D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor [Homo
sapiens].
Length = 343
Score = 287 bits (734), Expect = 2e-77
Identities = 144/183 (78%), Positives = 156/183 (85%)
Frame = +2
Query: 236 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 415
RLSRPLS+LP KTL+ +RENGAR LL S SF+P RRTYA + PVGSKAVLVTGCD
Sbjct: 5 RLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCD 64
Query: 416 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXX 595
SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSL SDRLRTVQLN
Sbjct: 65 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVV 124
Query: 596 XTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIR 775
+RSSL+DPEKG+WGLVNNAGISTFG+VEFTS+ETYK+VAEVNLWGTVR+TKSFLPLIR
Sbjct: 125 EIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIR 184
Query: 776 RAK 784
RAK
Sbjct: 185 RAK 187
>ref|NP_004042.1| D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor [Homo
sapiens].
Length = 343
Score = 287 bits (734), Expect = 2e-77
Identities = 144/183 (78%), Positives = 156/183 (85%)
Frame = +2
Query: 236 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 415
RLSRPLS+LP KTL+ +RENGAR LL S SF+P RRTYA + PVGSKAVLVTGCD
Sbjct: 5 RLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCD 64
Query: 416 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXX 595
SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSL SDRLRTVQLN
Sbjct: 65 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVV 124
Query: 596 XTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIR 775
+RSSL+DPEKG+WGLVNNAGISTFG+VEFTS+ETYK+VAEVNLWGTVR+TKSFLPLIR
Sbjct: 125 EIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIR 184
Query: 776 RAK 784
RAK
Sbjct: 185 RAK 187
>ref|NP_002144.1| estradiol 17-beta-dehydrogenase 2 [Homo sapiens].
Length = 387
Score = 98.6 bits (244), Expect = 1e-20
Identities = 59/137 (43%), Positives = 79/137 (57%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV KAVLVTG D G G +L K+L GF VFAG L ++ G +EL S RL +Q
Sbjct: 79 PVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVL--NENGPGAEELRRTCSPRLSVLQ 136
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTF-GDVEFTSMETYKEVAEVNLW 733
++ + + LQD +GLW ++NNAG+ F D E M YK+ VN +
Sbjct: 137 MDITKPVQIKDAYSKVAAMLQD--RGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFF 194
Query: 734 GTVRVTKSFLPLIRRAK 784
GTV VTK+FLPL+R++K
Sbjct: 195 GTVEVTKTFLPLLRKSK 211
>ref|NP_001135743.1| dehydrogenase/reductase SDR family member 9 precursor [Homo
sapiens].
Length = 319
Score = 92.4 bits (228), Expect = 7e-19
Identities = 52/139 (37%), Positives = 78/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
+ + K + +TGCDSGFG A+ KGF V A CL + G L + S+RLRT
Sbjct: 24 IEDITDKYIFITGCDSGFGNLAARTFDKKGFHVIAACLTE----SGSTALKAETSERLRT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVN 727
V L+ +++ Q EKGLWGL+NNAG+ ++ ++E Y+E EVN
Sbjct: 80 VLLDVTDPENVKRTAQWVKN--QVGEKGLWGLINNAGVPGVLAPTDWLTLEDYREPIEVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L+G + VT + LPL+++A+
Sbjct: 138 LFGLISVTLNMLPLVKKAQ 156
>ref|NP_954674.1| dehydrogenase/reductase SDR family member 9 precursor [Homo
sapiens].
Length = 319
Score = 92.4 bits (228), Expect = 7e-19
Identities = 52/139 (37%), Positives = 78/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
+ + K + +TGCDSGFG A+ KGF V A CL + G L + S+RLRT
Sbjct: 24 IEDITDKYIFITGCDSGFGNLAARTFDKKGFHVIAACLTE----SGSTALKAETSERLRT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVN 727
V L+ +++ Q EKGLWGL+NNAG+ ++ ++E Y+E EVN
Sbjct: 80 VLLDVTDPENVKRTAQWVKN--QVGEKGLWGLINNAGVPGVLAPTDWLTLEDYREPIEVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L+G + VT + LPL+++A+
Sbjct: 138 LFGLISVTLNMLPLVKKAQ 156
>ref|NP_001135742.1| dehydrogenase/reductase SDR family member 9 precursor [Homo
sapiens].
Length = 319
Score = 92.4 bits (228), Expect = 7e-19
Identities = 52/139 (37%), Positives = 78/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
+ + K + +TGCDSGFG A+ KGF V A CL + G L + S+RLRT
Sbjct: 24 IEDITDKYIFITGCDSGFGNLAARTFDKKGFHVIAACLTE----SGSTALKAETSERLRT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVN 727
V L+ +++ Q EKGLWGL+NNAG+ ++ ++E Y+E EVN
Sbjct: 80 VLLDVTDPENVKRTAQWVKN--QVGEKGLWGLINNAGVPGVLAPTDWLTLEDYREPIEVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L+G + VT + LPL+++A+
Sbjct: 138 LFGLISVTLNMLPLVKKAQ 156
>ref|NP_005762.2| dehydrogenase/reductase SDR family member 9 precursor [Homo
sapiens].
Length = 319
Score = 92.4 bits (228), Expect = 7e-19
Identities = 52/139 (37%), Positives = 78/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
+ + K + +TGCDSGFG A+ KGF V A CL + G L + S+RLRT
Sbjct: 24 IEDITDKYIFITGCDSGFGNLAARTFDKKGFHVIAACLTE----SGSTALKAETSERLRT 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVN 727
V L+ +++ Q EKGLWGL+NNAG+ ++ ++E Y+E EVN
Sbjct: 80 VLLDVTDPENVKRTAQWVKN--QVGEKGLWGLINNAGVPGVLAPTDWLTLEDYREPIEVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L+G + VT + LPL+++A+
Sbjct: 138 LFGLISVTLNMLPLVKKAQ 156
>ref|NP_003699.3| retinol dehydrogenase 16 [Homo sapiens].
Length = 317
Score = 92.0 bits (227), Expect = 9e-19
Identities = 55/133 (41%), Positives = 75/133 (56%), Gaps = 1/133 (0%)
Frame = +2
Query: 389 KAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXX 568
K V +TGCDSGFG LA+ L ++G V A CL + G ++L SDRL TV L+
Sbjct: 30 KYVFITGCDSGFGKLLARQLDARGLRVLAACLTE----KGAEQLRGQTSDRLETVTLDVT 85
Query: 569 XXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVNLWGTVR 745
++ ++D KGLWGLVNNAGIS E + + + + +VNL G +
Sbjct: 86 KTESVAAAAQWVKECVRD--KGLWGLVNNAGISLPTAPNELLTKQDFVTILDVNLLGVID 143
Query: 746 VTKSFLPLIRRAK 784
VT S LPL+RRA+
Sbjct: 144 VTLSLLPLVRRAR 156
>ref|NP_003716.2| 17-beta-hydroxysteroid dehydrogenase type 6 precursor [Homo
sapiens].
Length = 317
Score = 90.1 bits (222), Expect = 4e-18
Identities = 56/139 (40%), Positives = 78/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L ++G V A CL + G ++L SDRL T
Sbjct: 24 VSHLQDKYVFITGCDSGFGNLLARQLDARGLRVLAACLTE----KGAEQLRGQTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIST-FGDVEFTSMETYKEVAEVN 727
V L+ ++ + D +GLWGLVNNAGI T E+ + E + +VN
Sbjct: 80 VTLDVTKMESIAAATQWVKEHVGD--RGLWGLVNNAGILTPITLCEWLNTEDSMNMLKVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G ++VT S LPL+RRA+
Sbjct: 138 LIGVIQVTLSMLPLVRRAR 156
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 42,780,894
Number of extensions: 1103705
Number of successful extensions: 3861
Number of sequences better than 1.0e-05: 14
Number of HSP's gapped: 3837
Number of HSP's successfully gapped: 14
Length of query: 398
Length of database: 18,297,164
Length adjustment: 105
Effective length of query: 293
Effective length of database: 14,835,944
Effective search space: 4346931592
Effective search space used: 4346931592
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to RefSeqMP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601S-063956
(1196 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001116155.1| D-beta-hydroxybutyrate dehydrogenase, mitoch... 278 6e-75
Alignment gi|NP_780386.3| D-beta-hydroxybutyrate dehydrogenase, mitochond... 278 6e-75
Alignment gi|NP_032316.2| estradiol 17-beta-dehydrogenase 2 [Mus musculus]. 108 8e-24
Alignment gi|NP_059501.1| retinol dehydrogenase 7 [Mus musculus]. 94 2e-19
Alignment gi|NP_001144221.1| retinol dehydrogenase 7 [Mus musculus]. 94 2e-19
Alignment gi|NP_033066.1| retinol dehydrogenase 16 [Mus musculus]. 94 2e-19
Alignment gi|NP_536684.2| retinol dehydrogenase 1 (all trans) [Mus muscul... 94 3e-19
Alignment gi|NP_032315.2| corticosteroid 11-beta-dehydrogenase isozyme 2 ... 92 8e-19
Alignment gi|NP_694773.1| retinol dehydrogenase 9 [Mus musculus]. 91 2e-18
Alignment gi|NP_780721.1| dehydrogenase/reductase SDR family member 9 pre... 90 3e-18
>ref|NP_001116155.1| D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor [Mus
musculus].
Length = 343
Score = 278 bits (711), Expect = 6e-75
Identities = 138/183 (75%), Positives = 150/183 (81%)
Frame = +2
Query: 236 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 415
RLSRPLSQLP K L+ +RENG R +LLFY SF P +RRTYA + KA+L+TGCD
Sbjct: 5 RLSRPLSQLPGKALSVRDRENGTRHTLLFYPASFSPDTRRTYASQADAASGKAILITGCD 64
Query: 416 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXX 595
SGFGFSLAKHLHSKGFLVFAGCLMKDKG GVKELDSLKSDRLRT+QLN
Sbjct: 65 SGFGFSLAKHLHSKGFLVFAGCLMKDKGDAGVKELDSLKSDRLRTIQLNVCNSEEVEKAV 124
Query: 596 XTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIR 775
TIRS L+DPEKG+WGLVNNAGISTFG+VEFTSMETYKEVAEVNLWGTVR TKSFLPL+R
Sbjct: 125 ETIRSGLKDPEKGMWGLVNNAGISTFGEVEFTSMETYKEVAEVNLWGTVRTTKSFLPLLR 184
Query: 776 RAK 784
RAK
Sbjct: 185 RAK 187
>ref|NP_780386.3| D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor [Mus
musculus].
Length = 343
Score = 278 bits (711), Expect = 6e-75
Identities = 138/183 (75%), Positives = 150/183 (81%)
Frame = +2
Query: 236 RLSRPLSQLPRKTLNFSERENGARGSLLFYSPSFVPGSRRTYADSVNPVGSKAVLVTGCD 415
RLSRPLSQLP K L+ +RENG R +LLFY SF P +RRTYA + KA+L+TGCD
Sbjct: 5 RLSRPLSQLPGKALSVRDRENGTRHTLLFYPASFSPDTRRTYASQADAASGKAILITGCD 64
Query: 416 SGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQLNXXXXXXXXXXX 595
SGFGFSLAKHLHSKGFLVFAGCLMKDKG GVKELDSLKSDRLRT+QLN
Sbjct: 65 SGFGFSLAKHLHSKGFLVFAGCLMKDKGDAGVKELDSLKSDRLRTIQLNVCNSEEVEKAV 124
Query: 596 XTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNLWGTVRVTKSFLPLIR 775
TIRS L+DPEKG+WGLVNNAGISTFG+VEFTSMETYKEVAEVNLWGTVR TKSFLPL+R
Sbjct: 125 ETIRSGLKDPEKGMWGLVNNAGISTFGEVEFTSMETYKEVAEVNLWGTVRTTKSFLPLLR 184
Query: 776 RAK 784
RAK
Sbjct: 185 RAK 187
>ref|NP_032316.2| estradiol 17-beta-dehydrogenase 2 [Mus musculus].
Length = 381
Score = 108 bits (270), Expect = 8e-24
Identities = 64/137 (46%), Positives = 80/137 (58%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV KAVLVTG DSGFG LAKHL GF VFAG L DK G +EL S+RL +Q
Sbjct: 80 PVDQKAVLVTGADSGFGHGLAKHLDKLGFTVFAGVL--DKEGPGAEELRKHCSERLSVLQ 137
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFG-DVEFTSMETYKEVAEVNLW 733
++ + +QD KGLW +VNNAG+ D E M Y++ VN +
Sbjct: 138 MDVTKPEQIKDAHSKVTEKIQD--KGLWAVVNNAGVFHLPIDGELIPMSIYRKCMAVNFF 195
Query: 734 GTVRVTKSFLPLIRRAK 784
GTV VTK+FLPL+R++K
Sbjct: 196 GTVEVTKAFLPLLRKSK 212
>ref|NP_059501.1| retinol dehydrogenase 7 [Mus musculus].
Length = 316
Score = 94.0 bits (232), Expect = 2e-19
Identities = 54/138 (39%), Positives = 77/138 (55%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L + SDRL T
Sbjct: 24 VSHLQDKYVFITGCDSGFGNLLARQLDRRGMRVLAACLTEK----GAEQLRNKTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNL 730
V L+ ++ + + +GLWGLVNNAGI F E+ E + + +VNL
Sbjct: 80 VILDVTKTESIVAATQWVKERVGN--RGLWGLVNNAGICVFAINEWLKKEDFANILDVNL 137
Query: 731 WGTVRVTKSFLPLIRRAK 784
G + VT S LPL+R+A+
Sbjct: 138 LGMIEVTLSMLPLVRKAR 155
>ref|NP_001144221.1| retinol dehydrogenase 7 [Mus musculus].
Length = 316
Score = 94.0 bits (232), Expect = 2e-19
Identities = 54/138 (39%), Positives = 77/138 (55%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G ++L + SDRL T
Sbjct: 24 VSHLQDKYVFITGCDSGFGNLLARQLDRRGMRVLAACLTEK----GAEQLRNKTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGISTFGDVEFTSMETYKEVAEVNL 730
V L+ ++ + + +GLWGLVNNAGI F E+ E + + +VNL
Sbjct: 80 VILDVTKTESIVAATQWVKERVGN--RGLWGLVNNAGICVFAINEWLKKEDFANILDVNL 137
Query: 731 WGTVRVTKSFLPLIRRAK 784
G + VT S LPL+R+A+
Sbjct: 138 LGMIEVTLSMLPLVRKAR 155
>ref|NP_033066.1| retinol dehydrogenase 16 [Mus musculus].
Length = 317
Score = 94.0 bits (232), Expect = 2e-19
Identities = 57/139 (41%), Positives = 79/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G +EL + SDRL T
Sbjct: 24 VSHLQDKYVFITGCDSGFGTLLARQLDRRGMRVLAACLTE----KGAEELRNKTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIST-FGDVEFTSMETYKEVAEVN 727
V L+ ++ + + +GLWGLVNNAGIST G E+ + + V +VN
Sbjct: 80 VILDVTKTESIVTATQWVKEHVGN--RGLWGLVNNAGISTPSGPNEWMKKQDFAHVLDVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT S LPL+R+A+
Sbjct: 138 LLGMIEVTLSMLPLVRKAR 156
>ref|NP_536684.2| retinol dehydrogenase 1 (all trans) [Mus musculus].
Length = 317
Score = 93.6 bits (231), Expect = 3e-19
Identities = 57/139 (41%), Positives = 79/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G +EL + SDRL T
Sbjct: 24 VSHLQDKYVFITGCDSGFGNLLARQLDRRGMRVLAACLTE----KGAEELRNKTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIST-FGDVEFTSMETYKEVAEVN 727
V L+ ++ + + +GLWGLVNNAGIST G E+ + + V +VN
Sbjct: 80 VILDVTKTESIVAATQWVKERVGN--RGLWGLVNNAGISTPSGPNEWMKKQDFARVLDVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT S LPL+R+A+
Sbjct: 138 LLGMIEVTLSMLPLVRKAR 156
>ref|NP_032315.2| corticosteroid 11-beta-dehydrogenase isozyme 2 [Mus musculus].
Length = 386
Score = 92.0 bits (227), Expect = 8e-19
Identities = 53/137 (38%), Positives = 77/137 (56%), Gaps = 1/137 (0%)
Frame = +2
Query: 377 PVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQ 556
PV ++AVL+TGCD+GFG AK L + GF V A L D G EL L S RL+ +Q
Sbjct: 79 PVATRAVLITGCDTGFGKETAKKLDAMGFTVLATVL--DLNSPGALELRDLCSPRLKLLQ 136
Query: 557 LNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVNLW 733
++ ++ GLWGLVNNAG++ DVE + + T+++ EVN +
Sbjct: 137 MDLTKAEDISRVLEITKA--HTASTGLWGLVNNAGLNIVVADVELSPVATFRKCMEVNFF 194
Query: 734 GTVRVTKSFLPLIRRAK 784
G + +TK LPL+R ++
Sbjct: 195 GALELTKGLLPLLRHSR 211
>ref|NP_694773.1| retinol dehydrogenase 9 [Mus musculus].
Length = 317
Score = 90.9 bits (224), Expect = 2e-18
Identities = 56/139 (40%), Positives = 78/139 (56%), Gaps = 1/139 (0%)
Frame = +2
Query: 371 VNPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRT 550
V+ + K V +TGCDSGFG LA+ L +G V A CL + G +EL + SDRL T
Sbjct: 24 VSYLQDKYVFITGCDSGFGNLLARQLDRRGMRVLAACLTE----KGAEELRNKTSDRLET 79
Query: 551 VQLNXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGIS-TFGDVEFTSMETYKEVAEVN 727
V L+ ++ + + +GLWGLVNNAGIS G E+ + + V +VN
Sbjct: 80 VILDVTKTESIVTATQWVKERVGN--RGLWGLVNNAGISIPSGPNEWMKKQDFAHVLDVN 137
Query: 728 LWGTVRVTKSFLPLIRRAK 784
L G + VT S LPL+R+A+
Sbjct: 138 LLGLIEVTLSMLPLVRKAR 156
>ref|NP_780721.1| dehydrogenase/reductase SDR family member 9 precursor [Mus
musculus].
Length = 319
Score = 90.1 bits (222), Expect = 3e-18
Identities = 50/136 (36%), Positives = 76/136 (55%), Gaps = 1/136 (0%)
Frame = +2
Query: 380 VGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLKSDRLRTVQL 559
+ K V +TGCD+GFG A+ KGF V A CL + G L + S+RL TV L
Sbjct: 27 IADKYVFITGCDTGFGNLAARTFDKKGFRVIAACLTE----SGSAALKAKTSERLHTVLL 82
Query: 560 NXXXXXXXXXXXXTIRSSLQDPEKGLWGLVNNAGI-STFGDVEFTSMETYKEVAEVNLWG 736
+ ++S + EKGLWGL+NNAG+ ++ +++ Y+E EVNL+G
Sbjct: 83 DVTDPENVKKTAQWVKSHV--GEKGLWGLINNAGVLGVLAPTDWLTVDDYREPIEVNLFG 140
Query: 737 TVRVTKSFLPLIRRAK 784
+ VT + LPL+++A+
Sbjct: 141 LINVTLNMLPLVKKAR 156
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 36,186,562
Number of extensions: 920475
Number of successful extensions: 3197
Number of sequences better than 1.0e-05: 16
Number of HSP's gapped: 3154
Number of HSP's successfully gapped: 16
Length of query: 398
Length of database: 15,617,559
Length adjustment: 103
Effective length of query: 295
Effective length of database: 12,523,851
Effective search space: 3694536045
Effective search space used: 3694536045
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
Search to Sscrofa10_2
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 20110601S-063956
(1196 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Sscrofa_Chr13 377 e-101
>Sscrofa_Chr13
|| Length = 218635234
Score = 377 bits (190), Expect = e-101
Identities = 190/190 (100%)
Strand = Plus / Plus
Query: 1 gtgttcgtgtaggggaggctggaagccgcaggagcgggagacggtggaggggattttgag 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142184282 gtgttcgtgtaggggaggctggaagccgcaggagcgggagacggtggaggggattttgag 142184341
Query: 61 aagaaaactgcggtagcgacctcactcggtggtacaagaggggaacgctagtccctccga 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142184342 aagaaaactgcggtagcgacctcactcggtggtacaagaggggaacgctagtccctccga 142184401
Query: 121 aagccgggttactgcgaacgcaccagctgagcccttagtactgcaacccccaccctcctt 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142184402 aagccgggttactgcgaacgcaccagctgagcccttagtactgcaacccccaccctcctt 142184461
Query: 181 tggtttgtgg 190
||||||||||
Sbjct: 142184462 tggtttgtgg 142184471
Score = 307 bits (155), Expect = 7e-81
Identities = 155/155 (100%)
Strand = Plus / Plus
Query: 631 aggcttgtggggcctggttaacaatgcaggcatctcaacgttcggggacgtggaattcac 690
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142136388 aggcttgtggggcctggttaacaatgcaggcatctcaacgttcggggacgtggaattcac 142136447
Query: 691 cagcatggagacctacaaggaggtggcagaagtgaacctctggggcacggtgcgggtgac 750
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142136448 cagcatggagacctacaaggaggtggcagaagtgaacctctggggcacggtgcgggtgac 142136507
Query: 751 aaaatcctttctccctctcatccggagggccaaag 785
|||||||||||||||||||||||||||||||||||
Sbjct: 142136508 aaaatcctttctccctctcatccggagggccaaag 142136542
Score = 307 bits (155), Expect = 7e-81
Identities = 155/155 (100%)
Strand = Plus / Minus
Query: 631 aggcttgtggggcctggttaacaatgcaggcatctcaacgttcggggacgtggaattcac 690
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142252403 aggcttgtggggcctggttaacaatgcaggcatctcaacgttcggggacgtggaattcac 142252344
Query: 691 cagcatggagacctacaaggaggtggcagaagtgaacctctggggcacggtgcgggtgac 750
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142252343 cagcatggagacctacaaggaggtggcagaagtgaacctctggggcacggtgcgggtgac 142252284
Query: 751 aaaatcctttctccctctcatccggagggccaaag 785
|||||||||||||||||||||||||||||||||||
Sbjct: 142252283 aaaatcctttctccctctcatccggagggccaaag 142252249
Score = 287 bits (145), Expect = 7e-75
Identities = 145/145 (100%)
Strand = Plus / Minus
Query: 489 aggacaaagggcatgacggggtcaaggaactggacagcttgaagagtgatcgattaagaa 548
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142260673 aggacaaagggcatgacggggtcaaggaactggacagcttgaagagtgatcgattaagaa 142260614
Query: 549 ccgtccagctcaatgtctgcaaaagtgaagaagtagagaaagtggtggagaccatccgct 608
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142260613 ccgtccagctcaatgtctgcaaaagtgaagaagtagagaaagtggtggagaccatccgct 142260554
Query: 609 cgagcttgcaggaccctgagaaagg 633
|||||||||||||||||||||||||
Sbjct: 142260553 cgagcttgcaggaccctgagaaagg 142260529
Score = 234 bits (118), Expect = 9e-59
Identities = 118/118 (100%)
Strand = Plus / Plus
Query: 189 ggaggagcctgaccactctgcctcttatggccacaatgctgaccgcccgcctctccagac 248
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142193213 ggaggagcctgaccactctgcctcttatggccacaatgctgaccgcccgcctctccagac 142193272
Query: 249 ccctgtcgcagctcccaaggaaaaccctgaatttctctgagagagaaaatggagcaag 306
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142193273 ccctgtcgcagctcccaaggaaaaccctgaatttctctgagagagaaaatggagcaag 142193330
Score = 224 bits (113), Expect = 9e-56
Identities = 113/113 (100%)
Strand = Plus / Plus
Query: 379 ggttggcagcaaagctgtcctggtcacaggctgtgactctggatttgggttctcactggc 438
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142203751 ggttggcagcaaagctgtcctggtcacaggctgtgactctggatttgggttctcactggc 142203810
Query: 439 caagcatctgcattcaaaaggcttccttgtatttgctggctgcttgatgaagg 491
|||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142203811 caagcatctgcattcaaaaggcttccttgtatttgctggctgcttgatgaagg 142203863
Score = 153 bits (77), Expect = 3e-34
Identities = 77/77 (100%)
Strand = Plus / Plus
Query: 305 agaggctcgctgctgttttactctccctcctttgtccctggcagccgtcggacttatgct 364
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142202698 agaggctcgctgctgttttactctccctcctttgtccctggcagccgtcggacttatgct 142202757
Query: 365 gattcggtgaacccggt 381
|||||||||||||||||
Sbjct: 142202758 gattcggtgaacccggt 142202774
Score = 129 bits (65), Expect = 4e-27
Identities = 65/65 (100%)
Strand = Plus / Plus
Query: 941 agcagtaatgacaacaatcataaccattcctaacacgctgtgacccaggcagaacatgct 1000
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142150298 agcagtaatgacaacaatcataaccattcctaacacgctgtgacccaggcagaacatgct 142150357
Query: 1001 ctaaa 1005
|||||
Sbjct: 142150358 ctaaa 142150362
Score = 129 bits (65), Expect = 4e-27
Identities = 65/65 (100%)
Strand = Plus / Minus
Query: 941 agcagtaatgacaacaatcataaccattcctaacacgctgtgacccaggcagaacatgct 1000
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142238493 agcagtaatgacaacaatcataaccattcctaacacgctgtgacccaggcagaacatgct 142238434
Query: 1001 ctaaa 1005
|||||
Sbjct: 142238433 ctaaa 142238429
Score = 113 bits (57), Expect = 2e-22
Identities = 57/57 (100%)
Strand = Plus / Plus
Query: 784 agatttccaagaaacaacaacctcaaaatgtccaagtcatcaaggaccccatctgga 840
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142150141 agatttccaagaaacaacaacctcaaaatgtccaagtcatcaaggaccccatctgga 142150197
Score = 113 bits (57), Expect = 2e-22
Identities = 57/57 (100%)
Strand = Plus / Minus
Query: 784 agatttccaagaaacaacaacctcaaaatgtccaagtcatcaaggaccccatctgga 840
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 142238650 agatttccaagaaacaacaacctcaaaatgtccaagtcatcaaggaccccatctgga 142238594
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 31,377,927
Number of extensions: 217
Number of successful extensions: 217
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 217
Number of HSP's successfully gapped: 11
Length of query: 1196
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 1175
Effective length of database: 2,808,413,156
Effective search space: 3299885458300
Effective search space used: 3299885458300
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)