BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPL01_0058_C12
(1514 letters)
Database: RefSeq49_MP.fasta
30,036 sequences; 15,617,559 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_034100.2| granulocyte-macrophage colony-stimulating facto... 177 3e-44
Alignment gi|XP_001474061.2| PREDICTED: hypothetical protein LOC100045292... 102 8e-22
Alignment gi|NP_032396.1| interleukin-5 receptor subunit alpha precursor ... 92 1e-18
Alignment gi|NP_032382.1| interleukin-13 receptor subunit alpha-2 precurs... 65 1e-10
Alignment gi|NP_032395.1| interleukin-3 receptor subunit alpha precursor ... 64 3e-10
Alignment gi|NP_038591.1| cytokine receptor common subunit gamma precurso... 62 1e-09
>ref|NP_034100.2| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha precursor [Mus musculus].
Length = 388
Score = 177 bits (448), Expect = 3e-44
Identities = 110/354 (31%), Positives = 170/354 (48%), Gaps = 6/354 (1%)
Frame = +3
Query: 237 DQNASLHVTFDPKTMTLRWDCRE---NVTSGKCLMTTHKENSLVRKEIKGEECRCTFQDH 407
D ++L++TFDP T TL W C NVT C +T+ + + + + CRC F+
Sbjct: 37 DAGSALNLTFDPWTRTLTWACDTAAGNVTVTSCTVTSREAG--IHRRVSPFGCRCWFRRM 94
Query: 408 -SLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAAP 584
+LH GV+ V + L + N G G+ A+N +C I A F++C W +G AAP
Sbjct: 95 MALHHGVTLDVNGTVGGAAAHWRLSFVNEGAAGSGAENLTCEIRAARFLSCAWREGPAAP 154
Query: 585 NDIQYFLYIRDSKKKIERECPRYLSDSGTHV--GCHLQNISGLSFYSYFLVNGTSQKSGI 758
D++Y L + +S + R ++D G V C ++S L +Y +V G S +
Sbjct: 155 ADVRYSLRVLNSTG---HDVARCMADPGDDVITQCIANDLSLLGSEAYLVVTGRSGAGPV 211
Query: 759 QFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQSNI 938
+F D V+ K +ER PP ++T CN SHC + W P T +L+ R+FQ+ ++Q QS
Sbjct: 212 RFLDDVVATKALERLGPPRDVTASCNSSHCTVSWAPPSTWASLTARDFQF--EVQWQSAE 269
Query: 939 RRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXXXXXX 1118
S + ++V + + FPSP P H +K+R D R WG WS
Sbjct: 270 PGSTPRKVLVVEE--TRLAFPSPAPHGGHKVKVRAGDTRMKHWGEWSPAHPLEAEDTRVP 327
Query: 1119 XXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNENQHVDHESL 1280
+RF LFPPIP I+DK++++ V+ E+L
Sbjct: 328 GALLYAVTACAVLLCALALGVTCRRFEVTRRLFPPIPGIRDKVSDDVRVNPETL 381
>ref|XP_001474061.2| PREDICTED: hypothetical protein LOC100045292 [Mus musculus].
Length = 419
Score = 102 bits (254), Expect = 8e-22
Identities = 51/146 (34%), Positives = 82/146 (56%), Gaps = 2/146 (1%)
Frame = +3
Query: 492 GVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGT 671
G G+ A+N +C I A F++C W +G AAP D++Y L + +S + R ++D G
Sbjct: 228 GAAGSGAENLTCEIRAARFLSCAWREGPAAPADVRYSLRVLNSTG---HDVARCMADPGD 284
Query: 672 HV--GCHLQNISGLSFYSYFLVNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESH 845
V C ++S L +Y +V G S ++F D V+ K +ER +PP ++T CN SH
Sbjct: 285 DVITQCIANDLSLLGSEAYLVVTGRSGAGPVRFLDDVVATKALERLDPPRDVTASCNSSH 344
Query: 846 CFIQWEKPRTRKTLSDREFQYQLDIQ 923
C + W P T +L+ R+FQ+++ Q
Sbjct: 345 CTVSWAPPSTWASLTARDFQFEVQWQ 370
>ref|NP_032396.1| interleukin-5 receptor subunit alpha precursor [Mus musculus].
Length = 415
Score = 92.0 bits (227), Expect = 1e-18
Identities = 71/255 (27%), Positives = 113/255 (44%), Gaps = 26/255 (10%)
Frame = +3
Query: 411 LHGGVSFTVKV-------NIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADF------- 548
LH G + +V+ + W+S L + PG GT+ N +C +
Sbjct: 87 LHEGFAASVRTILKSSHTTLASSWVSAELK-APPGSPGTSVTNLTCTTHTVVSSHTHLRP 145
Query: 549 ----MNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDS-GTHVGCHLQN--ISGL 707
+ CTW G+ AP D QYFLY R + +C Y D+ + C I+
Sbjct: 146 YQVSLRCTWLVGKDAPEDTQYFLYYRFGV--LTEKCQEYSRDALNRNTACWFPRTFINSK 203
Query: 708 SFYSYFL-VNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKT 884
F + +NG+S+++ I+ FD + I++ NPP N+TV + +IQWEKP +
Sbjct: 204 GFEQLAVHINGSSKRAAIKPFDQLFSPLAIDQVNPPRNVTVEIESNSLYIQWEKPLS--A 261
Query: 885 LSDREFQYQLDIQRQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADA---- 1052
D F Y+L I N + +LI NK+ +T+++++R A +
Sbjct: 262 FPDHCFNYELKIYNTKNGHIQKEKLI------ANKF-ISKIDDVSTYSIQVRAAVSSPCR 314
Query: 1053 RRAEWGTWSQPVQFG 1097
WG WSQP+ G
Sbjct: 315 MPGRWGEWSQPIYVG 329
>ref|NP_032382.1| interleukin-13 receptor subunit alpha-2 precursor [Mus musculus].
Length = 383
Score = 65.1 bits (157), Expect = 1e-10
Identities = 43/179 (24%), Positives = 78/179 (43%), Gaps = 2/179 (1%)
Frame = +3
Query: 339 HKENSLVRKEIKGEECRCTFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQN 518
+K+ + K I+G+ R +H +G + WI + S+ G T Q+
Sbjct: 85 YKDGFDLNKGIEGK-IRTHLSEHCTNGS-------EVQSPWIEASYGISDEGSLETKIQD 136
Query: 519 FSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNI 698
C+ YN ++ C+W G+ +D Y ++ +C YL +VGC L N+
Sbjct: 137 MKCIYYNWQYLVCSWKPGKTVYSDTNYTMFFWYEGLDHALQCADYLQHDEKNVGCKLSNL 196
Query: 699 SGLSFYSYFL-VNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYC-NESHCFIQWEKP 869
+ +F+ VNG+S+ I+ +V L+ I + PP + + N ++W P
Sbjct: 197 DSSDYKDFFICVNGSSKLEPIRSSYTVFQLQNIVKPLPPEFLHISVENSIDIRMKWSTP 255
>ref|NP_032395.1| interleukin-3 receptor subunit alpha precursor [Mus musculus].
Length = 396
Score = 63.9 bits (154), Expect = 3e-10
Identities = 75/278 (26%), Positives = 111/278 (39%), Gaps = 24/278 (8%)
Frame = +3
Query: 249 SLHVTFDPKTMTLRWDCRE--NVTSGKCLMTTHKENSLVRKEIKGEECRCTFQDHSLHGG 422
+LH+ DP TL WD ++T+G K + G RC+FQ SL
Sbjct: 36 NLHI--DPAHYTLSWDPAPGADITTGAFC---RKGRDIFVWADPGL-ARCSFQSLSLCHV 89
Query: 423 VSFTVKVNIDQRWISETLVY--SNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQ 596
+FTV + D R ++ ++ + + G AAQ+ C ++ ++C W +G A D+
Sbjct: 90 TNFTVFLGKD-RAVAGSIQFPPDDDGDHEAAAQDLRCWVHEGQ-LSCQWERGPKATGDVH 147
Query: 597 YFLYIRDSK--KKIERECPRYLS---------DSGTHVGCHLQ---------NISGLSFY 716
Y ++ RD + RECP Y S G H GC L N L
Sbjct: 148 YRMFWRDVRLGPAHNRECPHYHSLDVNTAGPAPHGGHEGCTLDLDTVLGSTPNSPDLVPQ 207
Query: 717 SYFLVNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDR 896
VNG+ + + D+ + L+ E PP +TV CN S +W R
Sbjct: 208 VTITVNGSGRAGPVPCMDNTVDLQRAEVLAPP-TLTVECNGSEAHARWV---ARNRFHHG 263
Query: 897 EFQYQLDIQRQSNIRRSENQLIVVSGDLGNKYNFPSPH 1010
Y L
Animal-Genome cDNA SPL01_0058_C12
Animal-Genome cDNA SPL01_0058_C12
Search to RefSeqBP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPL01_0058_C12
(1514 letters)
Database: RefSeq49_BP.fasta
33,088 sequences; 17,681,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_002686644.1| PREDICTED: colony stimulating factor 2 recep... 440 e-123
Alignment gi|XP_002686633.1| PREDICTED: colony stimulating factor 2 recep... 146 4e-35
Alignment gi|XP_002697013.1| PREDICTED: interleukin 5 receptor, alpha-lik... 76 9e-14
Alignment gi|XP_872862.4| PREDICTED: interleukin 5 receptor, alpha-like [... 76 9e-14
Alignment gi|NP_001095766.1| interleukin-13 receptor subunit alpha-2 [Bos... 65 2e-10
Alignment gi|XP_002699641.1| PREDICTED: interleukin 13 receptor, alpha 2-... 65 2e-10
Alignment gi|XP_002684050.1| PREDICTED: interleukin 3 receptor, alpha-lik... 64 3e-10
>ref|XP_002686644.1| PREDICTED: colony stimulating factor 2 receptor alpha chain-like [Bos
taurus].
Length = 348
Score = 440 bits (1132), Expect = e-123
Identities = 203/296 (68%), Positives = 252/296 (85%)
Frame = +3
Query: 210 REHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECR 389
+E QDLP + NASL+V FDP+T L WDCREN +SG+C++ HKE ++K++K EC+
Sbjct: 11 QEQQDLPTVKPNASLNVKFDPQTTKLTWDCRENASSGECVLI-HKEKGPIKKKVKDSECQ 69
Query: 390 CTFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAK 569
CTFQD+SLHGGV+ TV+V+++QR +SE LVY+NPG EGTAAQNFSC+IY+ADFMNC+WAK
Sbjct: 70 CTFQDYSLHGGVTLTVEVHVNQRRLSEMLVYTNPGREGTAAQNFSCVIYDADFMNCSWAK 129
Query: 570 GQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQK 749
G+AAP+D+QYFLYIR SKK+IERECPRYL DSGTHVGCHLQ++SGL+ Y+YFLVNGTSQ+
Sbjct: 130 GRAAPDDVQYFLYIR-SKKRIERECPRYLKDSGTHVGCHLQDLSGLTSYNYFLVNGTSQE 188
Query: 750 SGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQ 929
+GIQFFDSVL+LKEIE+YNPP+NITV CNESHC I+WEKPRTR+ +REFQYQLDIQR+
Sbjct: 189 TGIQFFDSVLLLKEIEQYNPPDNITVQCNESHCLIRWEKPRTRQPWPNREFQYQLDIQRR 248
Query: 930 SNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFG 1097
+ SE + V GD GN+YNFP P +A HT+K+RTADAR+A WG WSQPV+FG
Sbjct: 249 VSGLGSEQVISNVFGDSGNRYNFPKPGSKAKHTVKIRTADARKAHWGAWSQPVEFG 304
>ref|XP_002686633.1| PREDICTED: colony stimulating factor 2 receptor alpha chain-like [Bos
taurus].
Length = 300
Score = 146 bits (369), Expect = 4e-35
Identities = 101/279 (36%), Positives = 130/279 (46%), Gaps = 8/279 (2%)
Frame = +3
Query: 549 MNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDS-GTHVGCHLQNIS-GLSFYSY 722
MNC+W +G AAP D+QY LY S EC Y+ DS GT V + + S RSE Q +YN PH
Sbjct: 264 LLGYTLQVNQSS---RSEPQ----------EYNVSIPH 288
>ref|NP_038591.1| cytokine receptor common subunit gamma precursor [Mus musculus].
Length = 369
Score = 62.0 bits (149), Expect = 1e-09
Identities = 59/242 (24%), Positives = 104/242 (42%), Gaps = 13/242 (5%)
Frame = +3
Query: 411 LHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNF-----SCLIYNADFMNCTW---A 566
L G S V ++ I L+ ++ E +A C ++N ++MNCTW +
Sbjct: 19 LRAGWSSKVLMSSANEDIKADLILTSTAPEHLSAPTLPLPEVQCFVFNIEYMNCTWNSSS 78
Query: 567 KGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQ 746
+ QA + Y + D+ +EC YL GC +Q + Y F+V
Sbjct: 79 EPQATNLTLHYRYKVSDN--NTFQECSHYLFSKEITSGCQIQK-EDIQLYQTFVVQLQDP 135
Query: 747 KSGIQFFDSVLMLKEIERYNPPNNITV-YCNESHCFIQWEKPRTRKTLSDREFQYQLDIQ 923
+ + L L+ + P N+T+ +ES ++W+ + + +R QY +Q
Sbjct: 136 QKPQRRAVQKLNLQNLVIPRAPENLTLSNLSESQLELRWKS----RHIKERCLQYL--VQ 189
Query: 924 RQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRT----ADARRAEWGTWSQPVQ 1091
+SN RS +LIV + +++ PS +T ++R+ +W WSQPV
Sbjct: 190 YRSNRDRSWTELIV---NHEPRFSLPSVDELKRYTFRVRSRYNPICGSSQQWSKWSQPVH 246
Query: 1092 FG 1097
+G
Sbjct: 247 WG 248
Database: RefSeq49_MP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 15,617,559
Number of sequences in database: 30,036
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 30036
Number of Hits to DB: 51,487,301
Number of extensions: 1396274
Number of successful extensions: 5874
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 5835
Number of HSP's successfully gapped: 7
Length of query: 504
Length of database: 15,617,559
Length adjustment: 106
Effective length of query: 398
Effective length of database: 12,433,743
Effective search space: 4948629714
Effective search space used: 4948629714
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
GCH + G + Y
Sbjct: 1 MNCSWTRGPAAPADVQYQLYSWTSLHGNVSECSHYVLDSAGTRVGCHFDELGEGHTTDDY 60
Query: 723 F-LVNGTSQKSGIQFFDSVLML-KEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDR 896
F LVNGTS S IQF DS + +E+YNPP N+TV N SH I+W+ P R +LS+
Sbjct: 61 FFLVNGTSSDSDIQFLDSPSVNGPSLEKYNPPANVTVQDNVSHFVIRWDAPAMRYSLSNS 120
Query: 897 EFQYQLDIQRQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTW 1076
+Y LDIQ+ + S+ + + +S NKY P HT++MR W W
Sbjct: 121 VLRYALDIQKMGSF--SKREPVFLSRQYKNKYRLPRADVTGEHTVRMRVQHVLFQIWSEW 178
Query: 1077 SQPVQFGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNEN 1256
S FG KRF + LFPPIPR+K +L
Sbjct: 179 SSTHHFGVPEENFLVVLIGLVVGATVLFGMALMFLC-KRFSLKKKLFPPIPRVKQELAGA 237
Query: 1257 QHVDHESLWEKFTH-DAGKADREDVLTVEE---VAQGPA 1361
E H + D ED+ TVEE +A PA
Sbjct: 238 FLASLEETASCGGHPPSASQDPEDIFTVEETLSLASAPA 276
>ref|XP_002697013.1| PREDICTED: interleukin 5 receptor, alpha-like [Bos taurus].
Length = 556
Score = 75.9 bits (185), Expect = 9e-14
Identities = 77/289 (26%), Positives = 111/289 (38%), Gaps = 24/289 (8%)
Frame = +3
Query: 459 WISETLV--YSNPGVE--------GTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLY 608
W+S L +PG E T A N++ L ++CTW G+ AP+D QYFLY
Sbjct: 251 WVSAELKAPIGSPGTEIVNLTCTTNTKADNYTHLRPYQVSLHCTWLVGKDAPDDTQYFLY 310
Query: 609 IRDSKKKIERECPRYLSDS-GTHVGCHLQ----NISGLSFYSYFLVNGTSQKSGIQFFDS 773
R + IE EC Y D +V C N G + + + VNG+S+ + I+ FD
Sbjct: 311 YR-YRSWIE-ECQEYSKDVLQRNVACWFPRTFINSKGRDYLAVY-VNGSSKGAAIKPFDQ 367
Query: 774 VLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQ-----SNI 938
+ L I+R N P N+T + I WEKP + F Y++ I
Sbjct: 368 LFALHAIDRVNSPANVTAEIKGTCLSIHWEKPVS--AFPSHCFDYEVKIYNAKKGYFQTE 425
Query: 939 RRSENQLIVVSGDLGNKY----NFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXX 1106
+ + N LI + D+ Y SP RAT W WS+P+ G
Sbjct: 426 KMTTNTLITIIDDVSKYYIQVRAAVSPVCRAT------------GLWSEWSRPIYVGKDD 473
Query: 1107 XXXXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNE 1253
+ LFPP+P K +N+
Sbjct: 474 QKPWTEWFLIAVMMTICFILLIFSLICRICHVWTKLFPPVPGPKSNIND 522
>ref|XP_872862.4| PREDICTED: interleukin 5 receptor, alpha-like [Bos taurus].
Length = 641
Score = 75.9 bits (185), Expect = 9e-14
Identities = 77/289 (26%), Positives = 111/289 (38%), Gaps = 24/289 (8%)
Frame = +3
Query: 459 WISETLV--YSNPGVE--------GTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLY 608
W+S L +PG E T A N++ L ++CTW G+ AP+D QYFLY
Sbjct: 251 WVSAELKAPIGSPGTEIVNLTCTTNTKADNYTHLRPYQVSLHCTWLVGKDAPDDTQYFLY 310
Query: 609 IRDSKKKIERECPRYLSDS-GTHVGCHLQ----NISGLSFYSYFLVNGTSQKSGIQFFDS 773
R + IE EC Y D +V C N G + + + VNG+S+ + I+ FD
Sbjct: 311 YR-YRSWIE-ECQEYSKDVLQRNVACWFPRTFINSKGRDYLAVY-VNGSSKGAAIKPFDQ 367
Query: 774 VLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQ-----SNI 938
+ L I+R N P N+T + I WEKP + F Y++ I
Sbjct: 368 LFALHAIDRVNSPANVTAEIKGTCLSIHWEKPVS--AFPSHCFDYEVKIYNAKKGYFQTE 425
Query: 939 RRSENQLIVVSGDLGNKY----NFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXX 1106
+ + N LI + D+ Y SP RAT W WS+P+ G
Sbjct: 426 KMTTNTLITIIDDVSKYYIQVRAAVSPVCRAT------------GLWSEWSRPIYVGKDD 473
Query: 1107 XXXXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNE 1253
+ LFPP+P K +N+
Sbjct: 474 QKPWTEWFLIAVMMTICFILLIFSLICRICHVWTKLFPPVPGPKSNIND 522
>ref|NP_001095766.1| interleukin-13 receptor subunit alpha-2 [Bos taurus].
Length = 380
Score = 65.1 bits (157), Expect = 2e-10
Identities = 36/143 (25%), Positives = 63/143 (44%), Ga
Animal-Genome cDNA SPL01_0058_C12
Animal-Genome cDNA SPL01_0058_C12
Search to RefSeqHP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPL01_0058_C12
(1514 letters)
Database: RefSeq49_HP.fasta
32,964 sequences; 18,297,164 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|NP_001155001.1| granulocyte-macrophage colony-stimulating fa... 417 e-116
Alignment gi|NP_758448.1| granulocyte-macrophage colony-stimulating facto... 417 e-116
Alignment gi|NP_006131.2| granulocyte-macrophage colony-stimulating facto... 417 e-116
Alignment gi|NP_001155003.1| granulocyte-macrophage colony-stimulating fa... 389 e-108
Alignment gi|NP_001155002.1| granulocyte-macrophage colony-stimulating fa... 360 2e-99
Alignment gi|NP_758450.1| granulocyte-macrophage colony-stimulating facto... 360 2e-99
Alignment gi|NP_758449.1| granulocyte-macrophage colony-stimulating facto... 358 1e-98
Alignment gi|NP_001155004.1| granulocyte-macrophage colony-stimulating fa... 305 1e-82
Alignment gi|NP_758452.1| granulocyte-macrophage colony-stimulating facto... 236 3e-62
Alignment gi|NP_002174.1| interleukin-3 receptor subunit alpha precursor ... 140 4e-33
>ref|NP_001155001.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform a precursor [Homo sapiens].
Length = 400
Score = 417 bits (1072), Expect = e-116
Identities = 198/378 (52%), Positives = 257/378 (67%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEPRLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQS 932
GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQYQLD+ R++
Sbjct: 202 GIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKN 261
Query: 933 NIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXXXX 1112
+EN LI VSGDL N+YNFPS PRA H++K+R AD R ps = 1/143 (0%)
Frame = +3
Query: 447 IDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSKK 626
+ W T S+ G T Q+ C+ YN ++ C+W G A D Y LY
Sbjct: 119 VQSSWSEATYWISSQGNLDTKIQDMDCVYYNWQYLLCSWKPGMGAHLDSNYRLYYWYEGL 178
Query: 627 KIERECPRYLSDSGTHVGCHLQNISGLSFYSYFL-VNGTSQKSGIQFFDSVLMLKEIERY 803
EC Y+ G ++GC + + +++ VNG+S+ I+ + L+ I +
Sbjct: 179 DHVLECIDYIQAKGKNIGCRFPYLESSDYKDFYICVNGSSESQLIRPSYFIFQLQNIVKP 238
Query: 804 NPPNNITVYCNESHCFIQWEKPR 872
PP+ +++ S ++W PR
Sbjct: 239 LPPDYLSLTMKNSEVNLKWSIPR 261
>ref|XP_002699641.1| PREDICTED: interleukin 13 receptor, alpha 2-like [Bos taurus].
Length = 380
Score = 65.1 bits (157), Expect = 2e-10
Identities = 36/143 (25%), Positives = 63/143 (44%), Gaps = 1/143 (0%)
Frame = +3
Query: 447 IDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSKK 626
+ W T S+ G T Q+ C+ YN ++ C+W G A D Y LY
Sbjct: 119 VQSSWSEATYWISSQGNLDTKIQDMDCVYYNWQYLLCSWKPGMGAHLDSNYRLYYWYEGL 178
Query: 627 KIERECPRYLSDSGTHVGCHLQNISGLSFYSYFL-VNGTSQKSGIQFFDSVLMLKEIERY 803
EC Y+ G ++GC + + +++ VNG+S+ I+ + L+ I +
Sbjct: 179 DHVLECIDYIQAKGKNIGCRFPYLESSDYKDFYICVNGSSESQLIRPSYFIFQLQNIVKP 238
Query: 804 NPPNNITVYCNESHCFIQWEKPR 872
PP+ +++ S ++W PR
Sbjct: 239 LPPDYLSLTMKNSEVNLKWSIPR 261
>ref|XP_002684050.1| PREDICTED: interleukin 3 receptor, alpha-like, partial [Bos taurus].
Length = 427
Score = 64.3 bits (155), Expect = 3e-10
Identities = 57/223 (25%), Positives = 94/223 (42%), Gaps = 8/223 (3%)
Frame = +3
Query: 642 CPRYLSDS-GTHVGCHLQNIS---GLSFYSYFLVNGTSQKSGIQFFDSVLMLKEIERYNP 809
CP+Y+ + G +V CH ++S FLVNGTS S I L+EIE
Sbjct: 199 CPKYMQNQQGVNVQCHFDSLSLQPERQTLFRFLVNGTSGGSKIPCRQMTHRLEEIEILAA 258
Query: 810 PNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQSNIRRSENQLIVVSGDLGNK 989
PN +CNE++ F++WE ++L + +Y+L+IQ+ +++ E + V G L N
Sbjct: 259 PNITKKWCNENYSFMEWEV----RSLFTEDIKYELEIQKGTDLAYKEETELKVLG-LRN- 312
Query: 990 YNFPSPHPRATHTLKMRTADARRAE---WGTWSQPVQFGXXXXXXXXXXXXXXXXXXXXX 1160
P P +T+K++ D+ E G WS P
Sbjct: 313 ---PGP-----YTVKIKAMDSLFFESRPAGQWSAPQHLDCGRKDASFPIWLVYLLAPLGA 364
Query: 1161 XXXXXXXXF-KRFLARHSLFPPIPRIKDKLNENQHVDHESLWE 1286
+R+ LFP +P +KD + ++ + W+
Sbjct: 365 LLLVGLVFLCRRWPLMQKLFPRVPHVKDPVGDSFQNERMMAWD 407
Score = 57.0 bits (136), Expect = 4e-08
Identities = 36/120 (30%), Positives = 63/120 (52%)
Frame = +3
Query: 255 HVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRCTFQDHSLHGGVSFT 434
++ DP+ L WD NV+ C + + +++K I E+ C+ D S ++T
Sbjct: 10 NLRIDPERKRLAWDQHGNVSEIGCYVNS----KILKKAI--EKPYCSLGDLSCEVK-NYT 62
Query: 435 VKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIR 614
VKV I + S + Y G AA N +C +++ D ++C+WA G+ AP ++QY Y++
Sbjct: 63 VKV-IKGQPFSAWIQYPKQGNPLAAADNLTCRVHDVDQLSCSWAVGEEAPANVQYHFYLK 121
Database: RefSeq49_BP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 17,681,374
Number of sequences in database: 33,088
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33088
Number of Hits to DB: 59,516,980
Number of extensions: 1669822
Number of successful extensions: 7229
Number of sequences better than 1.0e-05: 7
Number of HSP's gapped: 7165
Number of HSP's successfully gapped: 12
Length of query: 504
Length of database: 17,681,374
Length adjustment: 107
Effective length of query: 397
Effective length of database: 14,140,958
Effective search space: 5613960326
Effective search space used: 5613960326
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
W +WS+ ++FG
Sbjct: 262 TQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGN 321
Query: 1113 XXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNENQHVDHESLWEKF 1292
FKRFL LFPP+P+IKDKLN+N V+ E +WE+F
Sbjct: 322 LGSVYIYVLLIVGTLVCGIVLGFLFKRFLRIQRLFPPVPQIKDKLNDNHEVEDEIIWEEF 381
Query: 1293 THDAGKADREDVLTVEEV 1346
T + GK RE+VLTV+E+
Sbjct: 382 TPEEGKGYREEVLTVKEI 399
>ref|NP_758448.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform a precursor [Homo sapiens].
Length = 400
Score = 417 bits (1072), Expect = e-116
Identities = 198/378 (52%), Positives = 257/378 (67%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEPRLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQS 932
GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQYQLD+ R++
Sbjct: 202 GIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKN 261
Query: 933 NIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXXXX 1112
+EN LI VSGDL N+YNFPS PRA H++K+R AD R W +WS+ ++FG
Sbjct: 262 TQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGN 321
Query: 1113 XXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNENQHVDHESLWEKF 1292
FKRFL LFPP+P+IKDKLN+N V+ E +WE+F
Sbjct: 322 LGSVYIYVLLIVGTLVCGIVLGFLFKRFLRIQRLFPPVPQIKDKLNDNHEVEDEIIWEEF 381
Query: 1293 THDAGKADREDVLTVEEV 1346
T + GK RE+VLTV+E+
Sbjct: 382 TPEEGKGYREEVLTVKEI 399
>ref|NP_006131.2| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform a precursor [Homo sapiens].
Length = 400
Score = 417 bits (1072), Expect = e-116
Identities = 198/378 (52%), Positives = 257/378 (67%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEPRLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQS 932
GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQYQLD+ R++
Sbjct: 202 GIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKN 261
Query: 933 NIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXXXX 1112
+EN LI VSGDL N+YNFPS PRA H++K+R AD R W +WS+ ++FG
Sbjct: 262 TQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGN 321
Query: 1113 XXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNENQHVDHESLWEKF 1292
FKRFL LFPP+P+IKDKLN+N V+ E +WE+F
Sbjct: 322 LGSVYIYVLLIVGTLVCGIVLGFLFKRFLRIQRLFPPVPQIKDKLNDNHEVEDEIIWEEF 381
Query: 1293 THDAGKADREDVLTVEEV 1346
T + GK RE+VLTV+E+
Sbjct: 382 TPEEGKGYREEVLTVKEI 399
Animal-Genome cDNA SPL01_0058_C12
Animal-Genome cDNA SPL01_0058_C12
Search to RefSeqCP_Rel49
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPL01_0058_C12
(1514 letters)
Database: RefSeq49_CP.fasta
33,336 sequences; 18,874,504 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_851588.1| PREDICTED: similar to Granulocyte-macrophage co... 435 e-122
Alignment gi|XP_851626.1| PREDICTED: similar to interleukin 3 receptor, a... 138 1e-32
Alignment gi|XP_533759.2| PREDICTED: similar to interleukin 5 receptor, a... 81 2e-15
Alignment gi|NP_001003201.1| cytokine receptor common subunit gamma precu... 61 2e-09
Alignment gi|NP_001003075.1| interleukin-13 receptor subunit alpha-2 prec... 59 9e-09
Alignment gi|XP_538150.2| PREDICTED: similar to Interleukin-13 receptor a... 56 8e-08
>ref|XP_851588.1| PREDICTED: similar to Granulocyte-macrophage colony-stimulating
factor receptor alpha chain precursor (GM-CSF-R-alpha)
(GMR) (CDw116) (CD116 antigen) [Canis familiaris].
Length = 495
Score = 435 bits (1119), Expect = e-122
Identities = 218/405 (53%), Positives = 266/405 (65%), Gaps = 30/405 (7%)
Frame = +3
Query: 222 DLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRCTFQ 401
DL ++ SL++ FD + M L WDC+EN T +C+M HKE + +K EC C F
Sbjct: 12 DLTTVEGFPSLNMKFDSRRMELSWDCKENTTYLECVMI-HKEKGPIEIMLKENECHCKFP 70
Query: 402 DHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAA 581
D+SLH GV+F VKVN QR I ETLVY+N G +GTAAQNFSC IYNA+FMNCTWAKGQAA
Sbjct: 71 DYSLHEGVTFMVKVNSIQRPIPETLVYTNSGGKGTAAQNFSCFIYNANFMNCTWAKGQAA 130
Query: 582 PNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKSGIQ 761
P+D+QYFLYIRD K + ERECP YL DSGTHVGCHL+++SGL++Y+YFLVNGTSQ++GIQ
Sbjct: 131 PDDVQYFLYIRDLKYRRERECPHYLEDSGTHVGCHLEDLSGLAYYNYFLVNGTSQRTGIQ 190
Query: 762 FFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQSNIR 941
FFDS+L K+IE Y+PPNNI+V+CN SHC IQWEKP TR TLS+REF+YQLDIQR ++
Sbjct: 191 FFDSILYWKKIEIYSPPNNISVHCNVSHCLIQWEKPVTRHTLSNREFKYQLDIQRLDSVP 250
Query: 942 RSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXXXXXXX 1121
I VSG+ NKYNFPSP PRA HT+K+RT+D R WG+WSQPV+FG
Sbjct: 251 HPHGLSIEVSGNWENKYNFPSPEPRAKHTVKIRTSDTRIQRWGSWSQPVEFGSDDPEASL 310
Query: 1122 XXXXXXXXXXXXXXXXXXXXXFKR------------------------------FLARHS 1211
KR FLA
Sbjct: 311 VHIYMLVVLGTLVSALIIGCLLKREPSLNLPVYLLTASLSTGSSLGGYHVVSDVFLASRR 370
Query: 1212 LFPPIPRIKDKLNENQHVDHESLWEKFTHDAGKADREDVLTVEEV 1346
LFP IP +KDK N+NQ DH+ +WEKF D KAD E+++TVEEV
Sbjct: 371 LFPRIPHVKDKWNDNQPSDHQVIWEKFVSDPQKADNEEIVTVEEV 415
>ref|XP_851626.1| PREDICTED: similar to interleukin 3 NAME=RefSeqHP_Rel49_3>>ref|NP_001155003.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform g precursor [Homo sapiens].
Length = 410
Score = 389 bits (999), Expect = e-108
Identities = 185/354 (52%), Positives = 238/354 (67%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEPRLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQS 932
GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQYQLD+ R++
Sbjct: 202 GIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKN 261
Query: 933 NIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXXXX 1112
+EN LI VSGDL N+YNFPS PRA H++K+R AD R W +WS+ ++FG
Sbjct: 262 TQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGN 321
Query: 1113 XXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNENQHVDHE 1274
FKRFL LFPP+P+IKDKLN+N V+ E
Sbjct: 322 LGSVYIYVLLIVGTLVCGIVLGFLFKRFLRIQRLFPPVPQIKDKLNDNHEVEDE 375
>ref|NP_001155002.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform f precursor [Homo sapiens].
Length = 434
Score = 360 bits (924), Expect = 2e-99
Identities = 166/295 (56%), Positives = 215/295 (72%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEPRLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQS 932
GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQYQLD+ R++
Sbjct: 202 GIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKN 261
Query: 933 NIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFG 1097
+EN LI VSGDL N+YNFPS PRA H++K+R AD R W +WS+ ++FG
Sbjct: 262 TQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFG 316
Score = 72.8 bits (177), Expect = 8e-13
Identities = 32/54 (59%), Positives = 42/54 (77%)
Frame = +3
Query: 1185 FKRFLARHSLFPPIPRIKDKLNENQHVDHESLWEKFTHDAGKADREDVLTVEEV 1346
FKRFL LFPP+P+IKDKLN+N V+ E +WE+FT + GK RE+VLTV+E+
Sbjct: 380 FKRFLRIQRLFPPVPQIKDKLNDNHEVEDEIIWEEFTPEEGKGYREEVLTVKEI 433
>ref|NP_758450.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform c precursor [Homo sapiens].
Length = 333
Score = 360 bits (924), Expect = 2e-99
Identities = 166/295 (56%), Positives = 215/295 (72%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEP receptor, alpha precursor [Canis
familiaris].
Length = 639
Score = 138 bits (348), Expect = 1e-32
Identities = 97/290 (33%), Positives = 142/290 (48%), Gaps = 10/290 (3%)
Frame = +3
Query: 255 HVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRCTFQDHSLHGGVSFT 434
++ +P + L WD NV+ KC + + + K I C+ F L +FT
Sbjct: 227 NLRMEPGSRRLTWDLSGNVSEIKCFINS----KYITKAIDSRYCQ--FYVLPLCEVKNFT 280
Query: 435 VKVNIDQRWISETLVYSNPGVEG---TAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFL 605
+ V D + S L Y G EG AA+ C +++ DF+ C+W G+AAP D+QY L
Sbjct: 281 ISVKQDPPF-STGLQYVPRGAEGKPAAAARGLDCWVHDVDFLTCSWEVGRAAPGDVQYRL 339
Query: 606 YIRDSKKKIERECPRY-LSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKSGIQFFDSVLM 782
Y RD K E+ECPRY ++ G HV C ++S L + F VNGTS++SGI D +
Sbjct: 340 YWRDLKAYREQECPRYEANNRGVHVRCRFDDVSRLQRHIQFWVNGTSRRSGIPCSDLCVE 399
Query: 783 LKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLS--DREFQYQLDIQR----QSNIRR 944
L EIER +PP +IT CN+S+ ++W K LS + F Y+L IQ+ S +
Sbjct: 400 LPEIERLSPP-HITATCNKSYSMMEW------KVLSHFNHRFLYELQIQKGTDPASTEKL 452
Query: 945 SENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQF 1094
EN ++ YN + K++ R +W WS P F
Sbjct: 453 YENHFVI--------YN------PGNYVAKLKVQGFFRKDWSEWSAPQLF 488
>ref|XP_533759.2| PREDICTED: similar to interleukin 5 receptor, alpha isoform 1
precursor [Canis familiaris].
Length = 415
Score = 81.3 bits (199), Expect = 2e-15
Identities = 74/254 (29%), Positives = 114/254 (44%), Gaps = 25/254 (9%)
Frame = +3
Query: 411 LHGGVSFTVKVNI-------DQRWISETLVYSNPGVEGTAAQNFSCL---IYNAD----- 545
LH G S +V+ + W+S L + PG GT+ N +C ++N
Sbjct: 85 LHQGFSASVQTILWDGHSLLPSDWVSAELK-APPGSPGTSIVNLTCTTNTVFNNSNPLRP 143
Query: 546 ---FMNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDS-GTHVGCHLQNISGLSF 713
++CTW G+ AP D QYFLY R + EC Y D+ ++ C S
Sbjct: 144 YQVSLHCTWLVGKDAPEDTQYFLYYRYGS--MTEECQEYRKDTLKRNIACWFPRTFISSR 201
Query: 714 YSYFL---VNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKT 884
S L V G+S+ + I+ FD + L EI+R NPP N+T S IQW+KP +
Sbjct: 202 GSDSLAVQVKGSSKHTIIKPFDQLFALHEIDRINPPENVTAVIEGSGLSIQWKKPVSAFP 261
Query: 885 LSDREFQYQLDIQRQSNIRRSE---NQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADAR 1055
+ E++ ++ R++ + + N I + D+ +KY S RA + R +
Sbjct: 262 IHCFEYEVKIYNTRKNYFQTEKITTNAFISIIDDI-SKY---SIQVRAAVSSVCR----Q 313
Query: 1056 RAEWGTWSQPVQFG 1097
W WSQP+ G
Sbjct: 314 TGFWSEWSQPLHVG 327
>ref|NP_001003201.1| cytokine receptor common subunit gamma precursor [Canis lupus
familiaris].
Length = 373
Score = 61.2 bits (147), Expect = 2e-09
Identities = 54/221 (24%), Positives = 94/221 (42%), Gaps = 10/221 (4%)
Frame = +3
Query: 465 SETLVYSNPGVEGTAAQNFSCLIYNADFMNCTW-AKGQAAPNDIQYFLYIRDSKKKIERE 641
SETL V C ++N ++MNCTW + + P ++ + ++S +E
Sbjct: 47 SETL-----SVSSLPLPEVQCFVFNVEYMNCTWNSSSEPRPTNLTLHYWYKNSNDDKVQE 101
Query: 642 CPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKSGIQFFDSVLMLKEIERYNPPNNI 821
C YL GC LQ + Y F+V + + L L+ + P N+
Sbjct: 102 CGHYLFSREVTAGCWLQK-EEIHLYETFVVQLRDPREPRRQSTQKLKLQNLVIPWAPENL 160
Query: 822 TVY-CNESHCFIQWEKPRTRKTLSDREFQYQLD--IQRQSNIRRSENQLIVVSGDLGNKY 992
T++ +ES + W S+R + L+ +Q +S+ RS + V D N +
Sbjct: 161 TLHNLSESQLELSW---------SNRHLDHCLEHVVQYRSDWDRSWTEQSV---DHRNSF 208
Query: 993 NFPSPHPRATHTLKMRT------ADARRAEWGTWSQPVQFG 1097
+ PS + +T ++R+ A+R W WS P+ +G
Sbjct: 209 SLPSVDGQKFYTFRVRSRYNPLCGSAQR--WSEWSHPIHWG 247
>ref|NP_001003075.1| interleukin-13 receptor subunit alpha-2 precursor [Canis lupus
familiaris].
RLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQS 932
GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQYQLD+ R++
Sbjct: 202 GIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKN 261
Query: 933 NIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFG 1097
+EN LI VSGDL N+YNFPS PRA H++K+R AD R W +WS+ ++FG
Sbjct: 262 TQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFG 316
>ref|NP_758449.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform b precursor [Homo sapiens].
Length = 377
Score = 358 bits (918), Expect = 1e-98
Identities = 165/294 (56%), Positives = 214/294 (72%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEPRLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQS 932
GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQYQLD+ R++
Sbjct: 202 GIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKN 261
Query: 933 NIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQF 1094
+EN LI VSGDL N+YNFPS PRA H++K+R AD R W +WS+ ++F
Sbjct: 262 TQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEF 315
>ref|NP_001155004.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform h [Homo sapiens].
Length = 267
Score = 305 bits (780), Expect = 1e-82
Identities = 141/266 (53%), Positives = 186/266 (69%)
Frame = +3
Query: 549 MNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFL 728
MNCTWA+G AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFL
Sbjct: 1 MNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFL 60
Query: 729 VNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQY 908
VNGTS++ GIQFFDS+L K+IER+NPP+N+TV CN +HC ++W++PRT + LS +FQY
Sbjct: 61 VNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQY 120
Query: 909 QLDIQRQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPV 1088
QLD+ R++ +EN LI VSGDL N+YNFPS PRA H++K+R AD R W +WS+ +
Sbjct: 121 QLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAI 180
Query: 1089 QFGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFKRFLARHSLFPPIPRIKDKLNENQHVD 1268
+FG FKRFL LFPP+P+IKDKLN+N V+
Sbjct: 181 EFGSDDGNLGSVYIYVLLIVGTLVCGIVLGFLFKRFLRIQRLFPPVPQIKDKLNDNHEVE 240
Query: 1269 HESLWEKFTHDAGKADREDVLTVEEV 1346
E +WE+FT + GK RE+VLTV+E+
Sbjct: 241 DEIIWEEFTPEEGKGYREEVLTVKEI 266
>ref|NP_758452.1| granulocyte-macrophage colony-stimulating factor receptor subunit
alpha isoform e precursor [Homo sapiens].
Length = 233
Score = 236 bits (603), Expect = 3e-62
Identities = 1 Length = 386
Score = 59.3 bits (142), Expect = 9e-09
Identities = 33/140 (23%), Positives = 61/140 (43%), Gaps = 2/140 (1%)
Frame = +3
Query: 459 WISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSKKKIER 638
W T S G T Q+ C+ YN ++ C+W G D Y L+
Sbjct: 122 WAETTYWTSPQGNRETKIQDMDCVYYNWQYLVCSWKPGMGVHFDTNYQLFYWYEGLDHSA 181
Query: 639 ECPRYLSDSGTHVGCHLQNISGLSFYSYFL-VNGTSQKSGIQFFDSVLMLKEIERYNPPN 815
EC Y+ +G ++GC + + +++ VNG+S+ I+ + L+ I + PP+
Sbjct: 182 ECTDYIKVNGKNMGCRFPYLESSDYKDFYICVNGSSESQPIRPSYFIFQLQNIVKPMPPD 241
Query: 816 NITVYC-NESHCFIQWEKPR 872
+++ N ++W P+
Sbjct: 242 YLSLTVKNSEEINLKWNMPK 261
>ref|XP_538150.2| PREDICTED: similar to Interleukin-13 receptor alpha-1 chain precursor
(IL-13R-alpha-1) (IL-13RA-1) (CD213a1 antigen) [Canis
familiaris].
Length = 450
Score = 56.2 bits (134), Expect = 8e-08
Identities = 63/306 (20%), Positives = 116/306 (37%), Gaps = 25/306 (8%)
Frame = +3
Query: 504 TAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGC 683
+A C+ +N +M CTW G+ D Y LY S +C + G H+GC
Sbjct: 150 SAVTELQCVWHNLSYMKCTWLPGRNTSPDTNYTLYYWHSSLGKILQCEDIYRE-GQHIGC 208
Query: 684 H--LQNISGLSFYSYFLVNGTSQKSG-IQFFDSVLMLKEIERYNPPNNITVYCNESHCFI 854
L N+ SF + + +G I+ +++ L + +PP+ ++ + ++
Sbjct: 209 SFALTNLKDSSFEQHSVQIVVKDNAGKIRPSFNIVPLTSHVKPDPPHIKRLFFQNGNLYV 268
Query: 855 QWEKPRTRKTLSDREFQYQLDI---QRQSN----IRRSENQLIVVSGDLGNKYNF--PSP 1007
QW+ P + R YQ+++ Q ++N + ++ Q G+L F P
Sbjct: 269 QWKNP---QNFYSRCLSYQVEVNNSQTETNDIFYVEEAKCQNSEFEGNLEGTICFMVPGV 325
Query: 1008 HPRATHTLKMRTADARRAE-----WGTWSQPVQFGXXXXXXXXXXXXXXXXXXXXXXXXX 1172
P +T+++R + W WSQ + G
Sbjct: 326 LPDTLNTVRIRVRTNKLCYEDDKLWSNWSQAMSIGENTDPTFYITMLLATPVIVAGAIIV 385
Query: 1173 XXXXFKRFLARHSLFPPIP--------RIKDKLNENQHVDHESLWEKFTHDAGKADREDV 1328
KR + +FPPIP D+ ++ H ++EK T K + + V
Sbjct: 386 LLLYLKRL--KIIIFPPIPDPGKIFKEMFGDQNDDTLHWRKYDIYEKQT----KEETDSV 439
Query: 1329 LTVEEV 1346
+ +E +
Sbjct: 440 VLIENL 445
Database: RefSeq49_CP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,874,504
Number of sequences in database: 33,336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 33336
Number of Hits to DB: 62,042,203
Number of extensions: 1705398
Number of successful extensions: 7862
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 7772
Number of HSP's successfully gapped: 7
Length of query: 504
Length of database: 18,874,504
Length adjustment: 107
Effective length of query: 397
Effective length of database: 15,307,552
Effective search space: 6077098144
Effective search space used: 6077098144
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
12/194 (57%), Positives = 139/194 (71%)
Frame = +3
Query: 213 EHQDLPALDQNASLHVTFDPKTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRC 392
E DL + +SL+V FD +TM L WDC+EN T KC +T K+N +V + EC C
Sbjct: 23 EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTD-KKNRVVEPRLSNNECSC 81
Query: 393 TFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKG 572
TF++ LH GV+F V VN QR + L+Y N G EGTAAQNFSC IYNAD MNCTWA+G
Sbjct: 82 TFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARG 141
Query: 573 QAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFLVNGTSQKS 752
AP D+QYFLYIR+SK++ E CP Y+ DSGTHVGCHL N+SGL+ +YFLVNGTS++
Sbjct: 142 PTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREI 201
Query: 753 GIQFFDSVLMLKEI 794
GIQFFDS+L K+I
Sbjct: 202 GIQFFDSLLDTKKI 215
>ref|NP_002174.1| interleukin-3 receptor subunit alpha precursor [Homo sapiens].
Length = 378
Score = 140 bits (352), Expect = 4e-33
Identities = 105/365 (28%), Positives = 165/365 (45%), Gaps = 9/365 (2%)
Frame = +3
Query: 273 KTMTLRWDCRENVTSGKCLMTTHKENSLVRKEIKGEECRCTFQDHSLHGGVSFTVKVNID 452
K L WD NVT +C+ V C F SL ++TV+V
Sbjct: 35 KAQQLTWDLNRNVTDIECVKDADYSMPAVNNSY------CQFGAISLCEVTNYTVRVANP 88
Query: 453 --QRWISETLVYSNPGVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSKK 626
WI L N G A+N +C I++ DF++C+WA G AP D+QY LY+ + +
Sbjct: 89 PFSTWI---LFPENSGKPWAGAENLTCWIHDVDFLSCSWAVGPGAPADVQYDLYLNVANR 145
Query: 627 KIERECPRYLSDS-GTHVGCHLQNISGLSF---YSYFLVNGTSQKSGIQFFDSVLMLKEI 794
+ + EC Y +D+ GT +GC +IS LS S+ LV G S GI D ++ +I
Sbjct: 146 RQQYECLHYKTDAQGTRIGCRFDDISRLSSGSQSSHILVRGRSAAFGIPCTDKFVVFSQI 205
Query: 795 ERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQYQLDIQRQSNIRRSENQLIVVSG 974
E PP N+T CN++H F+ W+ ++ +R+F+Y+L IQ++ V++
Sbjct: 206 EILTPP-NMTAKCNKTHSFMHWK----MRSHFNRKFRYELQIQKRMQ--------PVITE 252
Query: 975 DLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPVQFGXXXXXXXXXXXXXXXXXXX 1154
+ ++ +F +P T+T+++R + WS P +F
Sbjct: 253 QVRDRTSFQLLNP-GTYTVQIRARERVYEFLSAWSTPQRFECDQEEGANTRAWRTSLLIA 311
Query: 1155 XXXXXXXXXXF---KRFLARHSLFPPIPRIKDKLNENQHVDHESLWEKFTHDAGKADRED 1325
F +R+L LFP IP +KD + ++ D +WE AGKA E+
Sbjct: 312 LGTLLALVCVFVICRRYLVMQRLFPRIPHMKDPIGDSFQNDKLVVWE-----AGKAGLEE 366
Query: 1326 VLTVE 1340
L E
Sbjct: 367 CLVTE 371
Database: RefSeq49_HP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 18,297,164
Number of sequences in database: 32,964
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 32964
Number of Hits to DB: 60,858,470
Number of extensions: 1672019
Number of successful extensions: 7171
Number of sequences better than 1.0e-05: 19
Number of HSP's gapped: 7103
Number of HSP's successfully gapped: 21
Length of query: 504
Length of database: 18,297,164
Length adjustment: 107
Effective length of query: 397
Effective length of database: 14,770,016
Effective search space: 5863696352
Effective search space used: 5863696352
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 36 (18.5 bits)
BLASTX 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPL01_0058_C12
(1514 letters)
Database: RefSeq49_SP.fasta
24,897 sequences; 11,343,932 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Alignment gi|XP_003362122.1| PREDICTED: granulocyte-macrophage colony-sti... 509 e-148
Alignment gi|XP_003358548.1| PREDICTED: interleukin-5 receptor subunit al... 81 1e-15
Alignment gi|NP_001230563.1| interleukin-13 receptor subunit alpha-2 [Sus... 57 3e-08
Alignment gi|NP_999248.2| cytokine receptor common subunit gamma [Sus scr... 55 1e-07
>ref|XP_003362122.1| PREDICTED: granulocyte-macrophage colony-stimulating factor receptor
subunit alpha-like, partial [Sus scrofa].
Length = 260
Score = 509 bits (1311), Expect(2) = e-148
Identities = 241/243 (99%), Positives = 242/243 (99%)
Frame = +3
Query: 369 IKGEECRCTFQDHSLHGGVSFTVKVNIDQRWISETLVYSNPGVEGTAAQNFSCLIYNADF 548
IKG+ECRCTFQDHSLHGGVSFTVKVNI QRWISETLVYSNPGVEGTAAQNFSCLIYNADF
Sbjct: 1 IKGKECRCTFQDHSLHGGVSFTVKVNIGQRWISETLVYSNPGVEGTAAQNFSCLIYNADF 60
Query: 549 MNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFL 728
MNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFL
Sbjct: 61 MNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFL 120
Query: 729 VNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQY 908
VNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQY
Sbjct: 121 VNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRKTLSDREFQY 180
Query: 909 QLDIQRQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPV 1088
QLDIQRQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPV
Sbjct: 181 QLDIQRQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADARRAEWGTWSQPV 240
Query: 1089 QFG 1097
QFG
Sbjct: 241 QFG 243
Score = 37.0 bits (84), Expect(2) = e-148
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +1
Query: 1192 GSSQGTAYSRRFHGSK 1239
GSSQGTAYSRRFHGSK
Sbjct: 243 GSSQGTAYSRRFHGSK 258
>ref|XP_003358548.1| PREDICTED: interleukin-5 receptor subunit alpha [Sus scrofa].
Length = 436
Score = 81.3 bits (199), Expect = 1e-15
Identities = 70/255 (27%), Positives = 110/255 (43%), Gaps = 26/255 (10%)
Frame = +3
Query: 411 LHGGVSFTVKVNI-------DQRWISETLVYSNPGVEGTAAQNFSCLIYNADF------- 548
LH G S +V+ + W+S L PG GT+ N +C +
Sbjct: 106 LHKGFSASVRTILWNNHSFPASSWVSAELKVP-PGSPGTSIVNLTCTTNTEAYNYTHERP 164
Query: 549 ----MNCTWAKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGT-HVGCHLQ----NIS 701
++CTW G+ AP D QYFLY R + EC Y D+ ++ C N
Sbjct: 165 YQVSLHCTWLVGKDAPEDTQYFLYYRYGS--VTEECREYSKDTQQRNIACRFPRTVINSK 222
Query: 702 GLSFYSYFLVNGTSQKSGIQFFDSVLMLKEIERYNPPNNITVYCNESHCFIQWEKPRTRK 881
G + + + VNG+S++ I+ FD + L I+R N P N+T + IQWEKP +
Sbjct: 223 GRDWLAVY-VNGSSKEEAIKPFDQLFALHAIDRVNSPVNVTAEIEGTCLSIQWEKPVSAF 281
Query: 882 TLSDREFQYQLDIQRQSNIRRSE---NQLIVVSGDLGNKYNFPSPHPRATHTLKMRTADA 1052
+ +++ ++ R+ + + N I + D+ +KY S RA + R
Sbjct: 282 PIHCFDYEVKICNTRKGYFQTEKMKTNTFISIIDDI-SKY---SVQVRAAVSSVCRPVGL 337
Query: 1053 RRAEWGTWSQPVQFG 1097
W WSQP+ G
Sbjct: 338 ----WSEWSQPIYVG 348
>ref|NP_001230563.1| interleukin-13 receptor subunit alpha-2 [Sus scrofa].
Length = 387
Score = 57.0 bits (136), Expect = 3e-08
Identities = 35/145 (24%), Positives = 65/145 (44%), Gaps = 3/145 (2%)
Frame = +3
Query: 447 IDQRWISETLVYSNP-GVEGTAAQNFSCLIYNADFMNCTWAKGQAAPNDIQYFLYIRDSK 623
+ W SET ++ P G T Q+ C+ YN ++ C+W G D Y L+
Sbjct: 119 VQSSW-SETTYWTLPQGNLETKIQDMDCVYYNWQYLLCSWKPGMGVHFDTNYNLFYWYEG 177
Query: 624 KKIERECPRYLSDSGTHVGCHLQNISGLSFYSYFL-VNGTSQKSGIQFFDSVLMLKEIER 800
+C Y+ +G + GC + + +++ VNG+S+ I+ + L+ I +
Sbjct: 178 LDHALQCADYIKTNGKNTGCRFPYLESSDYKDFYICVNGSSESQSIRPSYFIFQLQNIVK 237
Query: 801 YNPPNNITVYC-NESHCFIQWEKPR 872
PP+ +++ N ++W PR
Sbjct: 238 PLPPDYLSLTVKNSEEVNLKWSIPR 262
>ref|NP_999248.2| cytokine receptor common subunit gamma [Sus scrofa].
Length = 368
Score = 54.7 bits (130), Expect = 1e-07
Identities = 47/201 (23%), Positives = 86/201 (42%), Gaps = 10/201 (4%)
Frame = +3
Query: 525 CLIYNADFMNCTW-AKGQAAPNDIQYFLYIRDSKKKIERECPRYLSDSGTHVGCHLQNIS 701
C ++N ++MNCTW + + P ++ + + S +EC YL G GC
Sbjct: 62 CFVFNVEYMNCTWNSSSELQPTNLTLHYWYKTSNDDKVQECGHYLFSEGITSGCWFGK-E 120
Query: 702 GLSFYSYFLVNGTSQKSGIQFFDSVLMLKEIERYNPPNNITV-YCNESHCFIQWEKPRTR 878
+ Y F+V + + L L+++ P N+T+ +ES + W
Sbjct: 121 EIRLYQTFVVQLQDPREPRRQDPQTLKLQDLVIPWAPANLTLRTLSESQLELNW------ 174
Query: 879 KTLSDREFQYQLD--IQRQSNIRRSENQLIVVSGDLGNKYNFPSPHPRATHTLKMRT--- 1043
S+R + L+ +Q +S+ RS + V D ++ PS + +T ++R+
Sbjct: 175 ---SNRYLDHCLEHLVQYRSDRDRSWTEQSV---DHRQSFSLPSVDAQKLYTFRVRSRYN 228
Query: 1044 ---ADARRAEWGTWSQPVQFG 1097
A+R W WS P+ +G
Sbjct: 229 PLCGSAQR--WSDWSHPIHWG 247
Database: RefSeq49_SP.fasta
Posted date: Oct 17, 2011 1:42 PM
Number of letters in database: 11,343,932
Number of sequences in database: 24,897
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 24897
Number of Hits to DB: 38,525,115
Number of extensions: 1094902
Number of successful extensions: 4544
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 4521
Number of HSP's successfully gapped: 5
Length of query: 504
Length of database: 11,343,932
Length adjustment: 103
Effective length of query: 401
Effective length of database: 8,779,541
Effective search space: 3520595941
Effective search space used: 3520595941
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 35 (18.1 bits)
BLASTN 2.2.24 [Aug-08-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPL01_0058_C12
(1514 letters)
Database: Sscrofa_10.2.fasta
4582 sequences; 2,808,509,378 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GL896316.1| Sus scrofa unplaced genomic scaffold chrU_scaffol... 343 2e-91
gb|GL896511.1| Sus scrofa unplaced genomic scaffold chrU_scaffol... 341 7e-91
gb|GL896464.1| Sus scrofa unplaced genomic scaffold chrU_scaffol... 214 1e-52
>gb|GL896316.1| Sus scrofa unplaced genomic scaffold chrU_scaffold669
Length = 12820
Score = 343 bits (173), Expect = 2e-91
Identities = 173/173 (100%)
Strand = Plus / Plus
Query: 623 gaagaaaatcgagagagaatgtcctcggtacttatcagactcgggaacccacgtgggatg 682
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3443 gaagaaaatcgagagagaatgtcctcggtacttatcagactcgggaacccacgtgggatg 3502
Query: 683 tcacctccaaaacatctcgggattaagtttttacagttacttcctggttaacggtaccag 742
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3503 tcacctccaaaacatctcgggattaagtttttacagttacttcctggttaacggtaccag 3562
Query: 743 ccaaaagtcaggaatccagttctttgactcggttttgatgttaaaggaaatag 795
|||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3563 ccaaaagtcaggaatccagttctttgactcggttttgatgttaaaggaaatag 3615
Score = 274 bits (138), Expect = 1e-70
Identities = 138/138 (100%)
Strand = Plus / Plus
Query: 959 gattgtggtgtctggtgatttgggaaataagtacaactttccgagcccgcaccccagggc 1018
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 7604 gattgtggtgtctggtgatttgggaaataagtacaactttccgagcccgcaccccagggc 7663
Query: 1019 cacgcacaccctgaagatgagaacagcagacgcccggcgcgctgaatggggaacctggag 1078
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 7664 cacgcacaccctgaagatgagaacagcagacgcccggcgcgctgaatggggaacctggag 7723
Query: 1079 ccagcccgtccagttcgg 1096
||||||||||||||||||
Sbjct: 7724 ccagcccgtccagttcgg 7741
Score = 270 bits (136), Expect = 2e-69
Identities = 136/136 (100%)
Strand = Plus / Plus
Query: 488 cccaggtgtggagggcaccgccgcccaaaacttctcctgtcttatctacaacgcggattt 547
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3126 cccaggtgtggagggcaccgccgcccaaaacttctcctgtcttatctacaacgcggattt 3185
Query: 548 catgaactgcacctgggcaaagggccaagcagccccaaatgacatccaatattttttgta 607
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 3186 catgaactgcacctgggcaaagggccaagcagccccaaatgacatccaatattttttgta 3245
Query: 608 tatacgagactcaaag 623
||||||||||||||||
Sbjct: 3246 tatacgagactcaaag 3261
Score = 270 bits (136), Expect = 2e-69
Identities = 136/136 (100%)
Strand = Plus / Plus
Query: 794 agagagatacaaccctcccaacaacatcaccgtgtactgcaatgaatcccactgcttcat 853
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 5616 agagagatacaaccctcccaacaacatcaccgtgtactgcaatgaatcccactgcttcat 5675
Query: 854 ccagtgggaaaagcccaggacccgaaaaacactgtctgacagggagttccagtaccagct 913
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 5676 ccagtgggaaaagcccaggacccgaaaaacactgtctgacagggagttccagtaccagct 5735
Query: 914 ggacatccagagacag 929
||||||||||||||||
Sbjct: 5736 ggacatccagagacag 5751
Score = 238 bits (120), Expect = 7e-60
Identities = 126/128 (98%)
Strand = Plus / Plus
Query: 367 agatcaaaggcgaggaatgtcggtgtaccttccaagaccattctctccacgggggcgttt 426
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 485 agatcaaaggcaaggaatgtcggtgtaccttccaagaccattctctccacgggggcgttt 544
Query: 427 cgttcacggttaaagtaaacatcgaccaaagatggatttcagaaacgctggtctacagca 486
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
Sbjct: 545 cgttcacggttaaagtaaacatcggccaaagatggatttcagaaacgctggtctacagca 604
Query: 487 acccaggt 494
||||||||
Sbjct: 605 acccaggt 612
Score = 167 bits (84), Expect = 2e-38
Identities = 84/84 (100%)
Strand = Plus / Plus
Query: 1191 aggttcctcgcaaggcacagcttattcccgccgattccacggatcaaagacaaactgaac 1250
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 12289 aggttcctcgcaaggcacagcttattcccgccgattccacggatcaaagacaaactgaac 12348
Query: 1251 gagaaccaacacgtagaccacgag 1274
||||||||||||||||||||||||
Sbjct: 12349 gagaaccaacacgtagaccacgag 12372
Score = 63.9 bits (32), Expect = 2e-07
Identities = 32/32 (100%)
Strand = Plus / Plus
Query: 928 agagcaatatcagacgcagtgaaaatcaactg 959
||||||||||||||||||||||||||||||||
Sbjct: 6288 agagcaatatcagacgcagtgaaaatcaactg 6319
>gb|GL896511.1| Sus scrofa unplaced genomic scaffold chrU_scaffold844
Length = 5573
Score = 341 bits (172), Expect = 7e-91
Identities = 172/172 (100%)
Strand = Plus / Minus
Query: 1273 agagcctctgggagaagttcacacacgacgcagggaaggctgacagggaggacgtcttaa 1332
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 5479 agagcctctgggagaagttcacacacgacgcagggaaggctgacagggaggacgtcttaa 5420
Query: 1333 cggtggaagaagtggcccaaggccccgccagcgggtgaatttcagatgcctccacgcccc 1392
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 5419 cggtggaagaagtggcccaaggccccgccagcgggtgaatttcagatgcctccacgcccc 5360
Query: 1393 ggagtttcctggaaatcaattttccatttttcttcaatgtttccatcggttg 1444
||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 5359 ggagtttcctggaaatcaattttccatttttcttcaatgtttccatcggttg 5308
>gb|GL896464.1| Sus scrofa unplaced genomic scaffold chrU_scaffold801
Length = 11266
Score = 214 bits (108), Expect = 1e-52
Identities = 108/108 (100%)
Strand = Plus / Plus
Query: 1 ggaagataccgagctgaaatgtccttgtcatgcaatccgtgcggcagctctcagacgagg 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 5923 ggaagataccgagctgaaatgtccttgtcatgcaatccgtgcggcagctctcagacgagg 5982
Query: 61 aagagacctgtcagagggctcagatgtggggacagcgggacacagccc 108
||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 5983 aagagacctgtcagagggctcagatgtggggacagcgggacacagccc 6030
Score = 196 bits (99), Expect = 2e-47
Identities = 108/111 (97%)
Strand = Plus / Plus
Query: 222 gatcttcccgccctggaccagaacgccagtctccatgtgacgtttgacccaaagaccatg 281
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 10182 gatcttcccgccctggaccagaacgccagtctccatgtgacgtttgacccaaagaccatg 10241
Query: 282 acactaaggtgggactgcagggaaaatgtcacctctgggaagtgcctgatg 332
|||||||||||||||||||||||||| ||||||||||| |||||||||||
Sbjct: 10242 acactaaggtgggactgcagggaaaacatcacctctggggagtgcctgatg 10292
Score = 89.7 bits (45), Expect = 4e-15
Identities = 45/45 (100%)
Strand = Plus / Plus
Query: 118 cagtgcttgctcatcccgcaggctgccccaggcgtctcctaacca 162
|||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 9453 cagtgcttgctcatcccgcaggctgccccaggcgtctcctaacca 9497
Database: Sscrofa_10.2.fasta
Posted date: Nov 16, 2011 10:34 AM
Number of letters in database: 2,808,509,378
Number of sequences in database: 4582
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4582
Number of Hits to DB: 43,144,582
Number of extensions: 393
Number of successful extensions: 393
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 393
Number of HSP's successfully gapped: 11
Length of query: 1514
Length of database: 2,808,509,378
Length adjustment: 21
Effective length of query: 1493
Effective length of database: 2,808,413,156
Effective search space: 4192960841908
Effective search space used: 4192960841908
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 50 (99.1 bits)
S1: 18 (36.2 bits)
S2: 30 (60.0 bits)