Animal-Genome cDNA Clone 20030531/20030531C-0/20030531C-003954


Query

>20030531C-003954 (20030531C-003954) 20030531C-003954
CTGAGACAGA GGCCCGGCAC AACCTTCCTG TGTGGTTTCA GCAGCCAAGA
GTGACATGGA CCTGGGGAAG CCTATGAAGA AGAGCCTGCT GGTGGTGGCT
CTCCTTGTCA TTTTCCAGGT GTGCCTGTGC CAGGATGAGG TCACGGACGA
CTACATCGGC GACAACACCA CGGTGGACTA CACGCTGTAC GAGTCCGTGT
GCTTCAAGAA GGACGTGCGG ACCTTTAAGG CCTGGTTCCT CCCGGTCATG
TACTCCATCA TCTGCTTCGT GGGCCTGCTG GGCAACGGGC TGGTCATGCT
GACCTACATC TATTTCAAGA GGCTCAAGAC CATGACCGAC ACGTACCTGC
TCAACTTGGC CGTGGCCGAC ATCCTCTTCC TCCTGACCCT TCCCTTCTGG
GCATACAGCG CGGCCAAGTC CTGGGTCTTC GGAGTCCACG TCTGCAAACT
CATCTTCGGC ATCTACAAGA TCAGCTTCTT CAGCGGCATG CTTCTGCTTC
TCTGCATCAG CATCGACCGC TACGTGGCCA TCGTGCAGGC CGTGTCGGCG
CACCGCCACC GCGCCCGCGT GCTTCTCATC AGCAAGCTCT CCTGCGTGGG
CATCTGGATG CTGGCCATGG TGCTGTCCAC GCCGGAGCTG CTGTACAGCG
GCACCCAGAA GAGCAGCAGC GAGCAGGCGC TCCGGTGCTC CCTCATCACC
GAGCACGTGG AGGCCCTGAT CACCATCCAG GTGGCCCAGA TGGTGGTGGG
CTTTCTGATC CCCCTGGTGG CCATGAGCTT CTGCTACCTC GTCATCATCC
GCACCCTGCT CCAGGCGCGC AACTTCGAGC GCAACAAGGC CCATCAGGTG
ATCATCGCCG TGGTCGTGGT CTTCGTGGCC TTCCAGCTGC CCTACAACGG
CGTGGTCCTG GCCCAGACGG TGGCCAATTT CAACATCACC AGCGGCACCA
GCTG

Search to UniGene2 database

Query= 20030531C-003954 (20030531C-003954) 20030531C-003954
         (954 letters)

Database: UniGene(Bt+Hs+Ssc+Mm)
           230,285 sequences; 241,976,893 total letters

Searching..................................................done

                                                                               Score     E
            Sequences producing significant alignments:                        (bits)  Value

Alignment   gnl|UG|Ssc#S14562364 Sus scrofa CCR7 mRNA for chemokine recepto...  1737  0.0
Alignment   gnl|UG|Hs#S1726407 Homo sapiens chemokine (C-C motif) receptor ...   979  0.0
Alignment   gnl|UG|Mm#S8039354 Mus musculus chemokine (C-C motif) receptor ...   730  0.0
Alignment   gnl|UG|Hs#S1728959 Homo sapiens coagulation factor II (thrombin...    50  2e-04
Alignment   gnl|UG|Hs#S1731955 Homo sapiens galanin receptor 1 (GALR1), mRN...    46  0.003
Alignment   gnl|UG|Mm#S8041400 Mus musculus interleukin 8 receptor, beta (I...    44  0.012
Alignment   gnl|UG|Mm#S8042653 Mus musculus chemokine (C-C motif) receptor ...    44  0.012
Alignment   gnl|UG|Bt#S12073031 Bos taurus interleukin 8 receptor, beta (IL...    44  0.012
Alignment   gnl|UG|Hs#S1731688 Homo sapiens histamine receptor H3 (HRH3), m...    42  0.046
Alignment   gnl|UG|Hs#S1731944 Homo sapiens angiotensin II receptor-like 1 ...    42  0.046


>gnl|UG|Ssc#S14562364 Sus scrofa CCR7 mRNA for chemokine receptor 7,
           complete cds /cds=(1,1143) /gb=AB090356 /gi=28557109
           /ug=Ssc.16629 /len=1143
          Length = 1143

 Score = 1737 bits (876), Expect = 0.0
 Identities = 896/900 (99%), Gaps = 2/900 (0%)
 Strand = Plus / Plus


Query: 56  atggacctggggaagcctatgaagaagagcctgctggtggtggctctccttgtcattttc 115
           ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   atggacctggggaagccgatgaagaagagcctgctggtggtggctctccttgtcattttc 60


Query: 116 caggtgtgcctgtgccaggatgaggtcacggacgactacatcggcgacaacaccacggtg 175
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  caggtgtgcctgtgccaggatgaggtcacggacgactacatcggcgacaacaccacggtg 120


Query: 176 gactacacgctgtacgagtccgtgtgcttcaagaaggacgtgcggacctttaaggcctgg 235
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 gactacacgctgtacgagtccgtgtgcttcaagaaggacgtgcggacctttaaggcctgg 180


Query: 236 ttcctcccggtcatgtactccatcatctgcttcgtgggcctgctgggcaacgggctggtc 295
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ttcctcccggtcatgtactccatcatctgcttcgtgggcctgctgggcaacgggctggtc 240


Query: 296 atgctgacctacatctatttcaagaggctcaagaccatgaccgacacgtacctgctcaac 355
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 atgctgacctacatctatttcaagaggctcaagaccatgaccgacacgtacctgctcaac 300


Query: 356 ttggccgtggccgacatcctcttcctcctgacccttcccttctgggcatacagcgcggcc 415
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 ttggccgtggccgacatcctcttcctcctgacccttcccttctgggcatacagcgcggcc 360


Query: 416 aagtcctgggtcttcggagtccacgtctgcaaactcatcttcggcatctacaagatcagc 475
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 aagtcctgggtcttcggagtccacgtctgcaaactcatcttcggcatctacaagatcagc 420


Query: 476 ttcttcagcggcatgcttctgcttctctgcatcagcatcgaccgctacgtggccatcgtg 535
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 ttcttcagcggcatgcttctgcttctctgcatcagcatcgaccgctacgtggccatcgtg 480


Query: 536 caggccgtgtcggcgcaccgccaccgcgcccgcgtgcttctcatcagcaagctctcctgc 595
           |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Sbjct: 481 caggccgtgtcggcgcaccgccaccgtgcccgcgtgcttctcatcagcaagctctcctgc 540


Query: 596 gtgggcatctggatgctggccatggtgctgtccacgccggagctgctgtacagcggcacc 655
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 541 gtgggcatctggatgctggccatggtgctgtccacgccggagctgctgtacagcggcacc 600


Query: 656 cagaagagcagcagcgagcaggcgctccggtgctccctcatcaccgagcacgtggaggcc 715
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 601 cagaagagcagcagcgagcaggcgctccggtgctccctcatcaccgagcacgtggaggcc 660


Query: 716 ctgatcaccatccaggtggcccagatggtggtgggctttctgatccccctggtggccatg 775
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 661 ctgatcaccatccaggtggcccagatggtggtgggctttctgatccccctggtggccatg 720


Query: 776 agcttctgctacctcgtcatcatccgcaccctgctccaggcgcgcaacttcgagcgcaac 835
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 721 agcttctgctacctcgtcatcatccgcaccctgctccaggcgcgcaacttcgagcgcaac 780


Query: 836 aaggcccatc-aggtgatcatcgccgtggtcgtggtcttcgtggccttccagctgcccta 894
           |||| ||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 781 aagg-ccatcaaggtgatcatcgccgtggtcgtggtcttcgtggccttccagctgcccta 839


Query: 895 caacggcgtggtcctggcccagacggtggccaatttcaacatcaccagcggcaccagctg 954
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 840 caacggcgtggtcctggcccagacggtggccaatttcaacatcaccagcggcaccagctg 899



>gnl|UG|Hs#S1726407 Homo sapiens chemokine (C-C motif) receptor 7
           (CCR7), mRNA /cds=(67,1203) /gb=NM_001838 /gi=4502640
           /ug=Hs.1652 /len=2139
          Length = 2139

 Score =  979 bits (494), Expect = 0.0
 Identities = 829/936 (88%), Gaps = 6/936 (0%)
 Strand = Plus / Plus


Query: 9   gaggcccggcacaaccttcctgtgtggtttcagcagcca-agagtgacatggacctgggg 67
           |||||| |||||| |||||||||||||||| | |  ||| |||| | |||||||||||||
Sbjct: 19  gaggccgggcacagccttcctgtgtggttttaccgcccagagagcgtcatggacctgggg 78


Query: 68  aagcctatgaagaagagcctgctggtggtggctctccttgtcattttccaggtgtgcctg 127
           || || ||   ||| ||| |||||||||||||||||||||||||||||||||| ||||||
Sbjct: 79  aaaccaat---gaaaagcgtgctggtggtggctctccttgtcattttccaggtatgcctg 135


Query: 128 tgccaggatgaggtcacggacgactacatcggcgacaacaccacggtggactacacgctg 187
           || || ||||||||||||||||| |||||||| ||||||||||| |||||||||||  ||
Sbjct: 136 tgtcaagatgaggtcacggacgattacatcggagacaacaccacagtggactacactttg 195


Query: 188 tacgagtccgtgtgcttcaagaaggacgtgcggacctttaaggcctggttcctcccggtc 247
           | ||||||  |||||| ||||||||||||||||| |||||| ||||||||||||||  ||
Sbjct: 196 ttcgagtctttgtgctccaagaaggacgtgcggaactttaaagcctggttcctccctatc 255


Query: 248 atgtactccatcatctgcttcgtgggcctgctgggcaacgggctggtcatgctgacctac 307
           |||||||||||||| || ||||||||||| |||||||| ||||||||| || |||||||
Sbjct: 256 atgtactccatcatttgtttcgtgggcctactgggcaatgggctggtcgtgttgacctat 315


Query: 308 atctatttcaagaggctcaagaccatgaccgacacgtacctgctcaacttggccgtggcc 367
           |||||||||||||||||||||||||||||||| || |||||||||||| |||| |||||
Sbjct: 316 atctatttcaagaggctcaagaccatgaccgatacctacctgctcaacctggcggtggca 375


Query: 368 gacatcctcttcctcctgacccttcccttctgggcatacagcgcggccaagtcctgggtc 427
           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct: 376 gacatcctcttcctcctgacccttcccttctgggcctacagcgcggccaagtcctgggtc 435


Query: 428 ttcggagtccacgtctgcaaactcatcttcggcatctacaagatcagcttcttcagcggc 487
           ||||| |||||| | ||||| |||||||| | |||||||||||| ||||||||||| |||
Sbjct: 436 ttcggtgtccacttttgcaagctcatctttgccatctacaagatgagcttcttcagtggc 495


Query: 488 atgcttctgcttctctgcatcagcatcgaccgctacgtggccatcgtgcaggccgtgtcg 547
           ||||| || ||||| ||||||||||| |||||||||||||||||||| ||||| || ||
Sbjct: 496 atgctcctacttctttgcatcagcattgaccgctacgtggccatcgtccaggctgtctca 555


Query: 548 gcgcaccgccaccgcgcccgcgtgcttctcatcagcaagctctcctgcgtgggcatctgg 607
           || ||||||||||| |||||||| ||||||||||||||||| ||||| ||||||||||||
Sbjct: 556 gctcaccgccaccgtgcccgcgtccttctcatcagcaagctgtcctgtgtgggcatctgg 615


Query: 608 atgctggccatggtgctgtccacgccggagctgctgtacagcggcacccagaagagcagc 667
           || || ||||  ||||| ||||  || ||||| |||||||| | |  ||||| |||||||
Sbjct: 616 atactagccacagtgctctccatcccagagctcctgtacagtgacctccagaggagcagc 675


Query: 668 agcgagcaggcgctccggtgctccctcatcaccgagcacgtggaggccctgatcaccatc 727
           || ||||| ||| | || ||||| |||||||| ||||| ||||||||| | |||||||||
Sbjct: 676 agtgagcaagcgatgcgatgctctctcatcacagagcatgtggaggcctttatcaccatc 735


Query: 728 caggtggcccagatggtggtgggctttctgatccccctggtggccatgagcttctgctac 787
           |||||||||||||||||| | ||||||||| |||||||| |||||||||||||||| |||
Sbjct: 736 caggtggcccagatggtgatcggctttctggtccccctgctggccatgagcttctgttac 795


Query: 788 ctcgtcatcatccgcaccctgctccaggcgcgcaacttcgagcgcaacaaggcccatc-a 846
           || |||||||||||||||||||||||||| |||||||| ||||||||||||| ||||| |
Sbjct: 796 cttgtcatcatccgcaccctgctccaggcacgcaactttgagcgcaacaagg-ccatcaa 854


Query: 847 ggtgatcatcgccgtggtcgtggtcttcgtggccttccagctgccctacaacggcgtggt 906
           |||||||||||| ||||||||||||||| | | |||||||||||||||||| || |||||
Sbjct: 855 ggtgatcatcgctgtggtcgtggtcttcatagtcttccagctgccctacaatggggtggt 914


Query: 907 cctggcccagacggtggccaatttcaacatcaccag 942
           ||||||||||||||||||||| ||||||||||||||
Sbjct: 915 cctggcccagacggtggccaacttcaacatcaccag 950



>gnl|UG|Mm#S8039354 Mus musculus chemokine (C-C motif) receptor 7
            (Ccr7), mRNA /cds=(176,1312) /gb=NM_007719 /gi=6671771
            /ug=Mm.2932 /len=2072
          Length = 2072

 Score =  730 bits (368), Expect = 0.0
 Identities = 736/856 (85%), Gaps = 2/856 (0%)
 Strand = Plus / Plus


Query: 87   tgctggtggtggctctccttgtcattttccaggtgtgcctgtgccaggatgaggtcacgg 146
            |||||||||||||||||||||||||||||||||||||| | ||||| ||||||||||| |
Sbjct: 204  tgctggtggtggctctccttgtcattttccaggtgtgcttctgccaagatgaggtcaccg 263


Query: 147  acgactacatcggcgacaacaccacggtggactacacgctgtacgagtccgtgtgcttca 206
            | |||||||||||||| || ||||||||||||||||| ||||||||||| ||||||||||
Sbjct: 264  atgactacatcggcgagaataccacggtggactacaccctgtacgagtcggtgtgcttca 323


Query: 207  agaaggacgtgcggacctttaaggcctggttcctcccggtcatgtactccatcatctgct 266
            ||||||| ||||||| |||||||||||||||||| ||  ||||||| ||  |||||||||
Sbjct: 324  agaaggatgtgcggaactttaaggcctggttcctgcctctcatgtattctgtcatctgct 383


Query: 267  tcgtgggcctgctgggcaacgggctggtcatgctgacctacatctatttcaagaggctca 326
            ||||||||||||| |||||||||||||| || ||||| ||||||||||||||||||||||
Sbjct: 384  tcgtgggcctgctcggcaacgggctggtgatactgacgtacatctatttcaagaggctca 443


Query: 327  agaccatgaccgacacgtacctgctcaacttggccgtggccgacatcctcttcctcctga 386
            |||||||||| || || |||||||||||| |||||||||| |||||||| |||||||| |
Sbjct: 444  agaccatgacggatacctacctgctcaacctggccgtggcagacatccttttcctcctaa 503


Query: 387  cccttcccttctgggcatacagcgcggccaagtcctgggtcttcggagtccacgtctgca 446
              |||||||||||||| |||||||  |||||||||||| |||| || ||| || | || |
Sbjct: 504  ttcttcccttctgggcctacagcgaagccaagtcctggatctttggcgtctacctgtgta 563


Query: 447  aactcatcttcggcatctacaagatcagcttcttcagcggcatgcttctgcttctctgca 506
            |   |||||| |||||||| ||| | |||||||||||||| ||||| ||||| || ||||
Sbjct: 564  agggcatctttggcatctataagttaagcttcttcagcgggatgctgctgctcctatgca 623


Query: 507  tcagcatcgaccgctacgtggccatcgtgcaggccgtgtcggcgcaccgccaccgcgccc 566
            ||||||| ||||||||||| |||||||| ||||||||||||   || |||||||||||||
Sbjct: 624  tcagcattgaccgctacgtagccatcgtccaggccgtgtcgcgtcatcgccaccgcgccc 683


Query: 567  gcgtgcttctcatcagcaagctctcctgcgtgggcatctggatgctggccatggtgctgt 626
            |||||||||||||||||||||| ||||| ||||||||||||||||||||| |  | || |
Sbjct: 684  gcgtgcttctcatcagcaagctgtcctgtgtgggcatctggatgctggccctcttcctct 743


Query: 627  ccacgccggagctgctgtacagcggcacccagaagagcagcagcgagcaggcgctccggt 686
            |||  ||||||||||| |||||||||  |||||||| |||| ||||| |  ||||  | |
Sbjct: 744  ccatcccggagctgctctacagcggcctccagaagaacagcggcgaggacacgctgagat 803


Query: 687  gctccctcatcaccgagcacgtggaggccctgatcaccatccaggtggcccagatggtgg 746
            |||| ||  |||  |  || ||||||||| ||||||||||||| ||||||||||||||
Sbjct: 804  gctcactggtcagtgcccaagtggaggccttgatcaccatccaagtggcccagatggttt 863


Query: 747  tgggctttctgatccccctggtggccatgagcttctgctacctcgtcatcatccgcaccc 806
            | || || ||  | ||  || |||| ||||| |||||||||||| | |||||||| |||
Sbjct: 864  ttgggttcctagtgcctatgctggctatgagtttctgctacctcattatcatccgtacct 923


Query: 807  tgctccaggcgcgcaacttcgagcgcaacaaggcccatc-aggtgatcatcgccgtggtc 865
            |||||||||| |||||||| ||||| ||||||| ||||| |||||||||| ||||||||
Sbjct: 924  tgctccaggcacgcaactttgagcggaacaagg-ccatcaaggtgatcattgccgtggtg 982


Query: 866  gtggtcttcgtggccttccagctgccctacaacggcgtggtcctggcccagacggtggcc 925
            || |||||| | | |||||||||||||||||| || ||||||||||| ||||||||||||
Sbjct: 983  gtagtcttcatagtcttccagctgccctacaatggggtggtcctggctcagacggtggcc 1042


Query: 926  aatttcaacatcacca 941
            || |||||||||||||
Sbjct: 1043 aacttcaacatcacca 1058



>gnl|UG|Hs#S1728959 Homo sapiens coagulation factor II (thrombin)
           receptor-like 2 (F2RL2), mRNA /cds=(145,1269)
           /gb=NM_004101 /gi=4758325 /ug=Hs.194351 /len=1830
          Length = 1830

 Score = 50.1 bits (25), Expect = 2e-04
 Identities = 31/33 (93%)
 Strand = Plus / Plus


Query: 502 ctgcatcagcatcgaccgctacgtggccatcgt 534
           ||||||||||||| |||||||| ||||||||||
Sbjct: 699 ctgcatcagcatcaaccgctacctggccatcgt 731



>gnl|UG|Hs#S1731955 Homo sapiens galanin receptor 1 (GALR1), mRNA
            /cds=(773,1822) /gb=NM_001480 /gi=6031165 /ug=Hs.272191
            /len=3056
          Length = 3056

 Score = 46.1 bits (23), Expect = 0.003
 Identities = 23/23 (100%)
 Strand = Plus / Plus


Query: 515  gaccgctacgtggccatcgtgca 537
            |||||||||||||||||||||||
Sbjct: 1166 gaccgctacgtggccatcgtgca 1188



>gnl|UG|Mm#S8041400 Mus musculus interleukin 8 receptor, beta
           (Il8rb), mRNA /cds=(142,1221) /gb=NM_009909 /gi=6753455
           /ug=Mm.11756 /len=1858
          Length = 1858

 Score = 44.1 bits (22), Expect = 0.012
 Identities = 28/30 (93%)
 Strand = Plus / Plus


Query: 502 ctgcatcagcatcgaccgctacgtggccat 531
           |||||||||||| ||||||||| |||||||
Sbjct: 552 ctgcatcagcatggaccgctacctggccat 581



>gnl|UG|Mm#S8042653 Mus musculus chemokine (C-C motif) receptor 4
           (Ccr4), mRNA /cds=(136,1218) /gb=NM_009916 /gi=6857772
           /ug=Mm.1337 /len=1531
          Length = 1531

 Score = 44.1 bits (22), Expect = 0.012
 Identities = 40/46 (86%)
 Strand = Plus / Plus


Query: 316 caagaggctcaagaccatgaccgacacgtacctgctcaacttggcc 361
           ||||||||||||| ||||||| |||  ||||||||| ||| |||||
Sbjct: 336 caagaggctcaagtccatgacggacgtgtacctgctgaacctggcc 381



>gnl|UG|Bt#S12073031 Bos taurus interleukin 8 receptor, beta
           (IL8RB), mRNA /cds=(1,1083) /gb=NM_174360 /gi=27805942
           /ug=Bt.4208 /len=1083
          Length = 1083

 Score = 44.1 bits (22), Expect = 0.012
 Identities = 28/30 (93%)
 Strand = Plus / Plus


Query: 502 ctgcatcagcatcgaccgctacgtggccat 531
           |||||||||||| ||||||||| |||||||
Sbjct: 414 ctgcatcagcatggaccgctacctggccat 443



>gnl|UG|Hs#S1731688 Homo sapiens histamine receptor H3 (HRH3), mRNA
           /cds=(299,1636) /gb=NM_007232 /gi=6005781 /ug=Hs.251399
           /len=2699
          Length = 2699

 Score = 42.1 bits (21), Expect = 0.046
 Identities = 24/25 (96%)
 Strand = Plus / Plus


Query: 276 tgctgggcaacgggctggtcatgct 300
           |||||||||||| ||||||||||||
Sbjct: 444 tgctgggcaacgcgctggtcatgct 468



>gnl|UG|Hs#S1731944 Homo sapiens angiotensin II receptor-like 1
           (AGTRL1), mRNA /cds=(1,1143) /gb=NM_005161 /gi=4885056
           /ug=Hs.9305 /len=1143
          Length = 1143

 Score = 42.1 bits (21), Expect = 0.046
 Identities = 27/29 (93%)
 Strand = Plus / Plus


Query: 507 tcagcatcgaccgctacgtggccatcgtg 535
           ||||| ||||||||||| |||||||||||
Sbjct: 368 tcagcttcgaccgctacctggccatcgtg 396


  Database: UniGene(Bt+Hs+Ssc+Mm)
    Posted date:  May 6, 2003  5:18 PM
  Number of letters in database: 241,976,893
  Number of sequences in database:  230,285

Lambda     K      H
    1.37    0.711     1.31

Gapped
Lambda     K      H
    1.37    0.711     1.31


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 146887
Number of Sequences: 230285
Number of extensions: 146887
Number of successful extensions: 13152
Number of sequences better than 10.0: 47
length of query: 954
length of database: 241,976,893
effective HSP length: 19
effective length of query: 935
effective length of database: 237,601,478
effective search space: 222157381930
effective search space used: 222157381930
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 18 (36.2 bits)